SitesBLAST
Comparing 351332 FitnessBrowser__Btheta:351332 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 14 hits to proteins with known functional sites (download)
6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
41% identity, 87% coverage: 3:253/290 of query aligns to 1:225/262 of 6fahB
- binding flavin-adenine dinucleotide: C6 (≠ L8), A7 (= A9), K8 (= K10), D38 (= D42), T61 (= T65), M62 (= M66), A93 (= A97), D94 (= D98), T95 (= T99), T98 (= T102), L102 (= L106), G118 (= G122), Q120 (= Q124), G124 (= G128), T126 (= T130), A127 (= A131), V129 (= V133), T223 (= T251)
5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
42% identity, 93% coverage: 3:273/290 of query aligns to 1:242/260 of 5ol2B
- binding flavin-adenine dinucleotide: C6 (≠ L8), I7 (≠ A9), K8 (= K10), D38 (= D42), M61 (= M66), A92 (= A97), D93 (= D98), T94 (= T99), T97 (= T102), A115 (≠ G121), G116 (= G122), A119 (= A125), G122 (= G128), T124 (= T130), A125 (= A131), V127 (= V133)
7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
37% identity, 87% coverage: 4:256/290 of query aligns to 3:231/265 of 7qh2B
- binding flavin-adenine dinucleotide: C7 (≠ L8), K9 (= K10), D39 (= D42), M62 (= M66), D94 (= D98), V95 (≠ T99), L102 (= L106), G118 (= G122), Q120 (= Q124), T121 (≠ A125), T126 (= T130), A127 (= A131), V129 (= V133)
4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
48% identity, 63% coverage: 3:186/290 of query aligns to 1:180/263 of 4l2iB
- binding flavin-adenine dinucleotide: C6 (≠ L8), V7 (≠ A9), K8 (= K10), Y37 (≠ E41), D38 (= D42), Y40 (≠ N44), T60 (= T65), M61 (= M66), A92 (= A97), D93 (= D98), T94 (= T99), T97 (= T102), M101 (≠ L106), C116 (≠ G121), G117 (= G122), Q119 (= Q124), A120 (= A125), G123 (= G128), T125 (= T130), A126 (= A131), V128 (= V133)
- binding nicotinamide-adenine-dinucleotide: M61 (= M66), G62 (= G67), P63 (= P68), S85 (≠ T90), D86 (= D91), G91 (= G96)
4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
48% identity, 63% coverage: 3:186/290 of query aligns to 1:180/263 of 4kpuB
- binding flavin-adenine dinucleotide: C6 (≠ L8), V7 (≠ A9), K8 (= K10), Y37 (≠ E41), D38 (= D42), Y40 (≠ N44), M61 (= M66), A92 (= A97), D93 (= D98), T94 (= T99), T97 (= T102), C116 (≠ G121), G117 (= G122), Q119 (= Q124), A120 (= A125), G123 (= G128), T125 (= T130), A126 (= A131), V128 (= V133)
7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
36% identity, 86% coverage: 5:253/290 of query aligns to 4:232/282 of 7koeA
- binding flavin-adenine dinucleotide: C7 (≠ L8), I8 (≠ A9), K9 (= K10), D39 (= D42), M62 (= M66), A93 (= A97), D94 (= D98), T95 (= T99), T98 (= T102), L102 (= L106), T121 (≠ G121), G122 (= G122), A125 (= A125), G128 (= G128), T130 (= T130), G131 (≠ A131), V133 (= V133)
1efpB Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
35% identity, 63% coverage: 3:184/290 of query aligns to 1:181/246 of 1efpB
- binding adenosine monophosphate: P6 (≠ L8), V7 (≠ A9), D39 (= D42), I63 (≠ M66), V101 (≠ T102), A119 (≠ G121), G120 (= G122), Q122 (= Q124), A123 (= A125), N126 (≠ G128), M128 (≠ T130), N129 (≠ A131), T131 (≠ V133)
- binding flavin-adenine dinucleotide: Y13 (≠ T15)
Sites not aligning to the query:
Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
37% identity, 51% coverage: 38:184/290 of query aligns to 38:184/255 of Q2TBV3
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 200 modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- 203 modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
32% identity, 67% coverage: 38:231/290 of query aligns to 38:229/255 of Q9DCW4
- K200 (= K202) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (= K205) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
P38117 Electron transfer flavoprotein subunit beta; Beta-ETF from Homo sapiens (Human) (see 10 papers)
36% identity, 51% coverage: 38:184/290 of query aligns to 38:184/255 of P38117
- NPFC 39:42 (≠ NPED 39:42) binding
- C66 (≠ M66) binding
- 123:134 (vs. 122:133, 58% identical) binding
- D128 (= D127) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
- T154 (≠ L153) to M: in dbSNP:rs1130426
- R164 (= R164) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
- E165 (≠ H165) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
Sites not aligning to the query:
- 9 binding
- 183:205 Recognition loop
- 195 L→A: Severely impaired in complex formation with ACADM.
- 200 modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; K→R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- 200:202 KAK→RAR: Does not abolish methylation by ETFBKMT.
- 200:203 KAKK→RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- 203 modified: N6,N6,N6-trimethyllysine; by ETFBKMT; K→R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
36% identity, 51% coverage: 38:184/290 of query aligns to 35:181/252 of 2a1uB
- binding adenosine monophosphate: C39 (≠ D42), C63 (≠ M66), V101 (≠ L106), L119 (≠ G121), G120 (= G122), Q122 (= Q124), A123 (= A125), D126 (≠ G128), C128 (≠ T130), N129 (≠ A131), Q130 (= Q132), T131 (≠ V133)
- binding flavin-adenine dinucleotide: P37 (= P40)
Sites not aligning to the query:
1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
27% identity, 67% coverage: 3:197/290 of query aligns to 1:193/261 of 1o96A
- binding adenosine monophosphate: A6 (≠ L8), V7 (≠ A9), K8 (= K10), D38 (= D42), V63 (≠ M66), V99 (≠ T102), A117 (≠ G121), G118 (= G122), Q120 (= Q124), S121 (≠ A125), Y126 (≠ T130), A127 (= A131), T129 (≠ V133)
- binding flavin-adenine dinucleotide: Q120 (= Q124), Q182 (≠ N184)
P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
27% identity, 67% coverage: 3:197/290 of query aligns to 1:194/264 of P53570
5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
29% identity, 58% coverage: 3:169/290 of query aligns to 1:161/251 of 5ow0B
- binding adenosine monophosphate: L6 (= L8), A7 (= A9), K8 (= K10), D39 (= D42), Y62 (≠ M66), I95 (≠ T102), A114 (≠ G121), G115 (= G122), Q117 (= Q124), S118 (≠ A125), R123 (≠ T130), G124 (≠ A131), V125 (≠ Q132), V126 (= V133), P127 (≠ G134)
- binding flavin-adenine dinucleotide: L37 (≠ P40), Y38 (≠ E41)
Query Sequence
>351332 FitnessBrowser__Btheta:351332
MSLKIVVLAKQVPDTRNVGKDAMKADGTINRAALPAIFNPEDLNALEQALRLKDAHPGST
VTILTMGPGRAADIIREGLFRGADNGYLLTDRAFAGADTLATSYALATAIRKIGECDIII
GGRQAIDGDTAQVGPQVAEKLGLTQITYAEEILEVGDGKIKVKRHIDGGVETVEGPLPIV
ITVNGSAAPCRPRNAKLVQKYKHAKTVTEKQQGNLDYTDLYDKRDYLNLPEWSVADVNGD
LAQCGLSGSPTKVKAIQNIVFQAKESKTISGSDRDVEELIVELLANHTIG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory