SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 351389 FitnessBrowser__Btheta:351389 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9JZG1 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Neisseria meningitidis serogroup B (strain MC58) (see 2 papers)
44% identity, 99% coverage: 3:497/498 of query aligns to 5:503/517 of Q9JZG1

query
sites
Q9JZG1

N

N

R

R

L

V

F

I

I

I

F

F

D

D

T

T

L

L

R

R

D
|
D

G

G

E

E

Q

Q

V

S

P

P

G

G

C

A

Q

A

L

M

N

T

T

K

V

E

E

E

K

K

I

I

Q

R

V

V

A

A

K

R

A

Q

L

L

E

E

A

K

L

L

G

G

V

V

D

D

V

I

I

I

E

E

A

A

G

G

F

F

P

A

I

A

S

A

S

S

P

P

G

G

D

D

F

F

N

E

S

A

V

V

I

N

E

A

I

I

S

A

K

K

A

T

V

I

T

T

W

K

P

S

T

T

I

V

C

C

A

S

L

L

T

S

R

R

A

A

V

I

Q

E

K

R

D

D

I

I

D

R

V

Q

A

A

V

G

D

E

A

A

L

V

K

A

F

P

A

A

K

P

H

K

K

K

R

R

I

I

H

H

T

T

G

F

I

I

G

A

T

T

S

S

D

P

S

I

H

H

I

M

K

E

Y

Y

K

K

F

L

N

K

S

M

N

K

R

P

E

K

E

Q

I

V

I

I

E

E

R

A

A

A

V

V

A

K

A

A

V

V

K

K

Y

I

A

A

R

R

R

E

F

Y

V

T

D

D

V

V

E

E

F

F

Y

S

A

C

E

E

D

D

A

A

G

L

R

R

T

S

D

E

N

I

E

D

Y

F

L

L

A

A

R

E

V

I

V

C

E

G

A

A

V

V

I

I

K

E

A

A

G

G

A

A

T

T

V

T

V

I

N

N

I

I

P

P

D

D

T

T

T

V

G

G

Y

Y

C

S

L

I

P

P

S

Y

E

K

Y

T

G

E

A

E

K

F

I

F

K

R

Y

E

L

L

I

I

D

A

H

K

V

T

D

P

G

N

I

G

D

G

N

K

A

V

I

V

L

W

S

S

T

A

H
|
H

C

C

H
|
H

N

N

D

D

L

L

G

G

M

L

A

A

T

V

A

A

N

N

T

S

I

L

A

A

G

A

V

L

L

K

N

G

G

G

A

A

R

R

Q

Q

V

V

E

E

V

C

T

T

I

V

N

N

G

G

I

L

G

G

E

E

R

R

A

A

G

G

N
|
N

T

A

A

S

L

V

E

E

I

I

A

V

M

M

I

A

I

L

K

K

S

V

H

R

H

H

E

D

I

L

-

F

D

G

I

L

Q

E

T

T

N

G

I

I

N

D

T

T

Q

T

K

Q

I

I

Y

V

P

P

T

S

S

S

R

K

M

L

V

V

S

S

S

T

L

I

M

T

N

G

M

Y

P

P

V

V

Q

Q

P

P

N

N

K

K

A

A

I

I

V

V

G

G

R

A

N

N

A

A

F

F

A

S

H

H

S

E

S

S

G

G

I

I

H

H

Q

Q

D

D

G

G

V

V

L

L

K

K

N

H

V

R

E

E

T

T

Y

Y

E

E

I

I

I

M

D

S

P

A

H

E

D

S

V

V

G

G

I

W

D

A

D

T

N

N

S

R

I

L

V

S

L

L

T

G

A

K

R

L

S

S

G

G

R

R

A

N

A

A

L

F

K

K

N

T

R

K

L

L

S

A

L

D

L

L

G

G

V

I

N

E

L

L

D

E

-

S

Q

E

E

E

K

A

L

L

D

N

K

A

V

A

Y

F

E

A

E

R

F

F

L

K

K

E

L
|
L

A
|
A

D
|
D

K
|
K

K
x
R

D
x
E

I
|
I

N
x
F

D
|
D

D
x
E

D
|
D

V
x
L

L
x
H

V
x
A

L
|
L

A
x
V

G
x
S

A
x
D

D
x
E

R
x
M

S
x
G

Q
x
S

N
x
M

H
x
N

R
x
A

I
x
E

K
x
S

L
x
Y

E
x
K

Y
x
F

L
x
I

-
x
S

-
x
Q

Q
x
K

V
x
I

T
x
S

S
x
T

G
x
E

V
x
T

G
|
G

V
x
E

R
x
E

S
x
P

V
x
R

A
|
A

S
x
D

L
x
I

G
x
V

L
x
F

N
x
S

I
|
I

S
x
K

G
|
G

E
|
E

K
x
E

F
x
K

E
x
R

A
|
A

C
x
S

A
|
A

S
x
T

G
|
G

N
x
S

G
|
G

P
|
P

V
|
V

D
|
D

A
|
A

A
x
I

I
x
F

K
|
K

A
|
A

L
x
I

K
x
E

K
x
S

I
x
V

V
x
A

D
x
Q

R
x
S

H
x
G

M
x
A

T
x
A

L
|
L

K
x
Q

E
x
I

F
x
Y

T
x
S

I
x
V

Q
x
N

A
|
A

I
x
V

S
x
T

K
x
Q

G
|
G

S
x
T

D
x
E

D
x
S

V
x
Q

G
|
G

K
x
E

V
x
T

H
x
S

M
x
V

Q
x
R

V
x
L

E
x
A

Y
x
R

D
x
G

N
|
N

Q
x
R

I
x
V

Y
x
V

Y
x
N

G
|
G

F
x
Q

G
|
G

A
|
A

N
x
D

T
|
T

D
|
D

I
x
V

I
x
L

A
x
V

A
|
A

S
x
T

V
x
A

E
x
K

A
|
A

Y
|
Y

I
x
L

D
x
S

C
x
A

I
x
L

N
x
S

K
|
K

F
x
L

K
x
E

D16 (= D14) binding Mn(2+)
H204 (= H202) binding Mn(2+)
H206 (= H204) binding Mn(2+)
N240 (= N238) binding Mn(2+)

Sites not aligning to the query:

366:517 Required for the condensation reaction. Not required to bind substrate

6e1jA Crystal structure of methylthioalkylmalate synthase (bjumam1.1) from brassica juncea (see paper)
47% identity, 75% coverage: 3:377/498 of query aligns to 16:406/409 of 6e1jA

query
sites
6e1jA

N

N

R

Y

L

V

F

R

I

V

F

F

D

D

T

T

L

L

R

R

D
|
D

G

G

E

E

Q
|
Q

V

S

P

P

G

G

C

A

Q

A

L

L

N

T

T

P

V

P

E

Q

K

K

I

I

Q

E

V

I

A

A

K

R

A

Q

L

L

E

A

A

K

L

L

G

R

V

V

D

D

V

I

I

M

E

E

A

V

G

G

F
|
F

P

P

I

V

S
|
S

S

S

P

E

G

E

D

E

F

F

N

E

S

T

V

I

I

Q

E

T

I

I

S

A

K

K

A

T

V

V

-

G

-

N

-

E

-
x
V

-

D

-
x
E

-

E

-

T

-

G

T

Y

W

I

P

P

T

V

I

I

C

C

A

V

L
x
I

T

A

R
|
R

A

S

V

K

Q

E

K

R

D

D

I

I

D

K

V

A

A

A

V

W

D

E

A

S

L

V

K

K

F

Y

A

A

K

K

H

R

K

P

R

R

I

I

H

V

T

I

G
x
F

I

T

G

S

T

T

S

S

D

D

S

I

H

H

I

L

K

K

Y

Y

K
|
K

F
x
L

N

K

S

M

N

T

R

R

E

E

I

V

E

D

R

M

A

V

V

A

A

S

V

I

K

R

Y

F

A

A

R

K

R

S

F

L

-

G

V

F

D

E

D

D

V

I

E

E

F

F

Y

G

A

C

E

E

D

D

A

G

G

G

R

R

T

S

D

D

N

K

E

D

Y

Y

L

I

A

C

R

T

V

V

V

F

E

E

A

E

V

A

I

I

K

K

A

A

G

G

A

A

T

T

V

T

V

L

N

A

I

C

P
|
P

D

D

T
|
T

T

V

G

G

Y

I

C

N

L

M

P

P

S

H

E

E

Y

Y

G

G

A

K

K

L

I

V

K

R

Y

Y

L

I

I

K

D

A

H

N

V

T

D

P

G

G

I

I

D

D

N

D

A

V

I

I

L

F

S

S

T

A

H
|
H

C

C

H
|
H

N

N

D

D

L

L

G

G

M

V

A

A

T

T

A

A

N

N

T

T

I

I

A

A

G

G

V

I

L

C

N

A

G

G

A

A

R

R

Q

Q

V

V

E

E

V

V

T

T

I

I

N

N

G

G

I

I

G

G

E

E

R

R

A

S

G

G

N

N

T

A

A

P

L

L

E

E

I

V

A

V

M

M

I

A

I

L

K

K

S

C

H

R

H

G

E

A

I

F

-

V

-

M

-

G

D

G

I

V

Q

Y

T

T

N

R

I

I

N

D

T

T

Q

R

K

Q

I

I

Y

M

P

A

T

T

S

S

R

K

M

M

V

V

S

Q

S

E

L

Y

M

T

N

G

M

L

P

Y

V

V

Q

Q

P

P

N

H

K

K

A

P

I

I

V

V

G

G

R

A

N

N

A

C

F

F

A

V

H

H

S

E

S
|
S

G
|
G

I
|
I

H

H

Q

Q

D
|
D

G

G

V

I

L

L

K
|
K

N

N

V

R

E

S

T

T

Y

Y

E

E

I

I

D

S

P

P

H

E

D

D

V

V

G

G

I

V

-

V

-

K

-

S

D

Q

D

N

N

S

S

G

I

I

V

V

L

L

T

G

A

K

R
x
L

S

S

G

G

R
|
R

A
x
H

A

A

L

V

K

K

N

G

R

R

L

L

S

K

L

E

L

L

G

G

V

Y

N

E

L

I

D

S

Q

D

E

E

K

K

L

L

D

N

K

E

V

V

Y

F

E

S

E

R

F

F

L

R

K

D

L

L

A

T

D

K

K

Q

K

K

D

R

I

V

N

T

D

D

V

L

L

K

V

A

L

L

binding coenzyme a: Q30 (= Q17), F60 (= F47), S63 (= S50), I95 (≠ L73), R97 (= R75), F121 (≠ G99), K132 (= K110), L133 (≠ F111), S322 (= S296), G323 (= G297), I324 (= I298), D327 (= D301), K331 (= K305), L359 (≠ R330), R362 (= R333), H363 (≠ A334)
binding 4-(methylsulfanyl)-2-oxobutanoic acid: P192 (= P169), T194 (= T171), H225 (= H202), H227 (= H204)
binding manganese (ii) ion: D27 (= D14), V82 (vs. gap), E84 (vs. gap), H225 (= H202), H227 (= H204)

Q9FG67 Methylthioalkylmalate synthase 1, chloroplastic; 2-isopropylmalate synthase 3; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
45% identity, 80% coverage: 3:399/498 of query aligns to 83:491/506 of Q9FG67

query
sites
Q9FG67

N

N

R

Y

L

V

F

R

I

V

F

F

D

D

T

T

L

L

R

R

D

D

G

G

E

E

Q

Q

V

S

P

P

G

G

C

G

Q
x
S

L

L

N

T

T

P

V

P

E

Q

K

K

I

L

Q

E

V

I

A

A

K

R

A

Q

L

L

E

A

A

K

L

L

G

R

V

V

D

D

V

I

I

M

E

E

A

V

G

G

F

F

P

P

I

G

S

S

P

E

G

E

D

E

F

L

N

E

S

T

V

I

I

K

E

T

I

I

S

A

K

K

A

T

V

V

-

G

-

N

-

E

-

V

-

D

-

E

-

T

-

G

T

Y

W

V

P

P

T

V

I

I

C

C

A

A

L

I

T

A

R

R

A

C

V

K

Q

H

K

R

D

D

I

I

D

E

V

A

A

T

V

W

D

E

A

A

L

L

K

K

F

Y

A

A

K

K

H

R

K

P

R

R

I

I

H

L

T

V

G

F

I

T

G

S

T

T

S

S

D

D

S

I

H

H

I

M

K

K

Y

Y

K

K

F

L

N

K

S

K

N

T

R

Q

E

E

I

V

I

I

E

E

R

M

A

A

V

V

A

S

V

I

K

R

Y

F

A

A

R

K

R

S

F

L

-

G

V

F

D

N

D

D

V

I

E

Q

F

F

Y

G

A

C

E

E

D

D

A

G

G

G

R

R

T

S

D

D

N

K

E

D

Y

F

L

L

A

C

R

K

V

I

V

L

E

G

A

E

V

A

I

I

K

K

A

A

G

G

A

V

T

T

V

V

N

T

I

I

P

G

D

D

T

T

T

V

G

G

Y

I

C

N

L

M

P

P

S

H

E

E

Y

Y

G

G

A

E

K

L

I

V

K

T

Y

Y

L

L

I

K

D

A

H

N

V

T

D

P

G

G

I

I

D

D

N

D

A

V

I

V

L

V

S
x
A

T

V

H

H

C

C

H

H

N

N

D

D

L

L

G

G

M

L

A

A

T

T

A

A

N

N

T

S

I

I

A

A

G

G

V

I

L

R

N

A

G

G

A

A

R

R

Q

Q

V

V

E

E

V

V

T

T

I

I

N

N

G

G

I

I

G

G

E

E

R

R

A

S

G

G

N

N

T

A

A

S

L

L

E

E

I

V

A

V

M

M

I

A

I

L

K

K

S

C

H

R

H

G

E

A

I

Y

D

V

I

I

Q

N

-

G

-

V

-

Y

T

T

N

K

I

I

N

D

T

T

Q

R

K

Q

I

I

Y

M

P

A

T

T

S

S

R

K

M

M

V

V

S

Q

S

E

L

Y

M

T

N

G

M

L

P

Y

V

V

Q

Q

P

A

N

H

K

K

A

P

I

I

V

V

G

G

R

A

N

N

A

C

F

F

A

V

H

H

S

E

S

S

G

G

I

I

H

H

Q

Q

D

D

G

G

V

I

L

L

K

K

N

N

V

R

E

S

T

T

Y

Y

E

E

I

I

I

L

D

S

P

P

H

E

D

D

V

I

G

G

I

I

-

V

-

K

-

S

D

Q

D

N

N

S

S

G

I

L

V

V

L

L

T

G

A

K

R

L

S

S

G

G

R

R

A

H

A

A

L

V

K

K

N

D

R

R

L

L

S

K

L

E

L

L

G

G

V

Y

N

E

L

L

D

D

Q

D

E

E

K

K

L

L

D

N

K

A

V

V

Y

F

E

S

E

L

F

F

L

R

K

D

L

L

A

T

D

K

K

N

K

K

D

R

I

I

N

T

D

D

D

A

D

D

V

L

L

K

V

A

L

L

A

V

G

-

A

-

D

-

R

-

S

T

Q

S

N

S

H

D

R

E

I

I

K

S

L

L

E

E

Y

K

L

L

Q

N

V

G

T

A

S

N

G

G

V

L

S102 (≠ Q22) mutation to F: In gsm1-1; loss of conversion of C3 to C4 glucosinolates.
A290 (≠ S200) mutation to T: In gsm1-2; loss of conversion of C3 to C4 glucosinolates.

Q9FN52 Methylthioalkylmalate synthase 3, chloroplastic; 2-isopropylmalate synthase 2; Methylthioalkylmalate synthase-like; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
45% identity, 77% coverage: 3:383/498 of query aligns to 83:481/503 of Q9FN52

query
sites
Q9FN52

N

N

R

Y

L

V

F

R

I

V

F

L

D

D

T

T

L

L

R

R

D

D

G

G

E

E

Q

Q

V

S

P

P

G

G

C

A

Q

A

L

L

N

T

T

P

V

P

E

Q

K

K

I

L

Q

E

V

I

A

A

K

R

A

Q

L

L

E

A

A

K

L

L

G

R

V

V

D

D

V

I

I

M

E

E

A

V

G

G

F

F

P

P

I

V

S

S

P

E

G

E

D

E

F

F

N

E

S

A

V

I

I

K

E

T

I

I

S

A

K

K

A

T

V

V

-

G

-

N

-

E

-

V

-

D

-

E

-

T

-

G

T

Y

W

V

P

P

T

V

I

I

C

C

A

G

L

I

T

A

R

R

A

C

V

K

Q

K

K

R

D

D

I

I

D

E

V

A

A

T

V

W

D

E

A

A

L

L

K

K

F

Y

A

A

K

K

H

R

K

P

R

R

I

V

H

M

T

L

G

F

I

T

G

S

T

T

S

S

D

E

S

I

H

H

I

M

K

K

Y

Y

K

K

F

L

N

K

S

K

N

T

R

K

E

E

I

V

I

I

E

E

R

M

A

A

V

V

A

N

A

S

V

V

K

K

Y

Y

A

A

R

K

R

S

F

L

-

G

V

F

D

K

D

D

V

I

E

Q

F

F

Y

G

A

C

E

E

D

D

A

G

G

G

R

R

T

T

D

E

N

K

E

D

Y

F

L

I

A

C

R

K

V

I

V

L

E

G

A

E

V

S

I

I

K

K

A

A

G

G

A

A

T

T

V

T

V

V

N

G

I

F

P

A

D

D

T

T

T

V

G
|
G

Y

I

C

N

L

M

P

P

S

Q

E

E

Y

F

G

G

A

E

K

L

I

V

K

A

Y

Y

L

V

I

I

D

E

H

N

V

T

D

P

G

G

I

A

D

D

N

D

A

I

I

V

L

F

S

A

T

I

H

H

C

C

H

H

N

N

D

D

L

L

G

G

M

V

A

A

T

T

A

A

N

N

T

T

I

I

A

S

G

G

V

I

L

C

N

A

G

G

A

A

R

R

Q

Q

V

V

E

E

V

V

T

T

I

I

N

N

G

G

I

I

G

G

E

E

R

R

A

S

G

G

N

N

T

A

A

P

L

L

E

E

I

V

A

V

M

M

I

A

I

L

K

K

S

C

H

R

H

G

E

E

-

S

-

L

I

M

D

D

-

G

I

V

Q

Y

T

T

N

K

I

I

N

D

T

S

Q

R

K

Q

I

I

Y

M

P

A

T

T

S

S

R

K

M

M

V

V

S

Q

S

E

L

H

M

T

N

G

M

M

P

Y

V

V

Q

Q

P

P

N

H

K

K

A

P

I

I

V

V

G

G

R

D

N

N

A

C

F

F

A

V

H

H

S

E

S

S

G

G

I

I

H

H

Q

Q

D

D

G

G

V

I

L

L

K

K

N

N

V

R

E

S

T

T

Y

Y

E

E

I

I

I

L

D

S

P

P

H

E

D

D

V

V

G

G

I

I

-

V

-

K

-

S

D

E

D

N

N

S

S

G

I

I

V

V

L

L

T

G

A

K

R

L

S

S

G

G

R

R

A

H

A

A

L

V

K

K

N

D

R

R

L

L

S

K

L

E

L

L

G

G

V

Y

N

E

L

I

D

S

Q

D

E

E

K

K

L

F

D

N

K

D

V

I

Y

F

E

S

E

R

F

Y

L

R

K

E

L

L

A

T

D

K

K

D

K

K

D

R

I

I

N

T

D

D

D

A

D

D

V

L

-

K

-

A

L

L

V

V

L

V

A

N

G

G

A

A

D

E

R

I

S

S

G263 (= G173) mutation to E: In gsm2-1; loss of activity and lack of C6, C7 and C8 aliphatic glucosinolates.

3rmjB Crystal structure of truncated alpha-isopropylmalate synthase from neisseria meningitidis (see paper)
50% identity, 64% coverage: 3:319/498 of query aligns to 2:306/308 of 3rmjB

query
sites
3rmjB

N

N

R

R

L

V

F

I

I

I

F

F

D

D

T

T

L

L

R

R

D
|
D

G

G

E

E

Q
|
Q

V

S

P

P

G

G

C

A

Q

A

L

M

N

T

T

K

V

E

E

E

K

K

I

I

Q

R

V

V

A

A

K

R

A

Q

L

L

E

E

A

K

L

L

G

G

V

V

D

D

V

I

I

I

E

E

A

A

G

G

F

F

P

A

I

A

S

A

S

S

P

P

G

G

D

D

F

F

N

E

S

A

V

V

I

N

E

A

I

I

S

A

K

K

A

T

V

I

T

T

W

K

P

S

T

T

I

V

C

C

A

S

L

L

T

S

R

R

A

A

V

I

Q

E

K

R

D

D

I

I

D

R

V

Q

A

A

V

G

D

E

A

A

L

V

K

A

F

P

A

A

K

P

H

K

K

K

R

R

I

I

H

H

T

T

G

F

I

I

G

A

T

T

S

S

D

P

S

I

H

H

I

M

K

E

Y

Y

K

K

F

L

N

K

S

M

N

K

R

P

E

K

E

Q

I

V

I

I

E

E

R

A

A

A

V

V

A

K

A

A

V

V

K

K

Y

I

A

A

R

R

R

E

F

Y

V

T

D

D

V

V

E

E

F

F

Y

S

A

C

E

E

D

D

A

A

G

L

R

R

T

S

D

E

N

I

E

D

Y

F

L

L

A

A

R

E

V

I

V

C

E

G

A

A

V

V

I

I

K

E

A

A

G

G

A

A

T

T

V

T

V

I

N

N

I

I

P

P

D

D

T

T

T

V

G

G

Y

Y

C

S

L

I

P

P

S

Y

E

K

Y

T

G

E

A

E

K

F

I

F

K

R

Y

E

L

L

I

I

D

A

H

K

V

T

D

P

G

N

I

G

D

G

N

K

A

V

I

V

L

W

S

S

T

A

H
|
H

C

C

H
|
H

N

N

D

D

L

L

G

G

M

L

A

A

T

V

A

A

N

N

T

S

I

L

A

A

G

A

V

L

L

K

N

G

G

G

A

A

R

R

Q

Q

V

V

E

E

V

C

T

T

I

V

N

N

G

G

I

L

G

G

E

E

R

R

A

A

G

G

N
|
N

T

A

A

S

L

V

E

E

I

I

A

V

M

M

I

A

I

L

K

K

S

V

H

R

H

H

E

D

I

L

-

F

D

G

I

L

Q

E

T

T

N

G

I

I

N

D

T

T

Q

T

K

Q

I

I

Y

V

P

P

T

S

S

S

R

K

M

L

V

V

S

S

S

T

L

I

M

T

N

G

M

Y

P

P

V

V

Q

Q

P

P

N

N

K

K

A

A

I

I

V

V

G

G

R

A

N

N

A

A

F

F

A

S

H

H

S

-

G

-

I

-

H

-

Q

-

D

-

G

-

V

-

L

-

K

-

N

-

V

-

E

E

T

T

Y

Y

E

E

I

I

I

M

D

S

P

A

H

E

D

S

V

V

G

G

active site: Q16 (= Q17)
binding manganese (ii) ion: D13 (= D14), H201 (= H202), H203 (= H204), N237 (= N238)

6ktqA Crystal structure of catalytic domain of homocitrate synthase from sulfolobus acidocaldarius (sahcs(dram)) in complex with alpha- ketoglutarate/zn2+/coa (see paper)
36% identity, 70% coverage: 7:355/498 of query aligns to 24:365/399 of 6ktqA

query
sites
6ktqA

I

I

F

L

D

D

T

S

T

T

L

L

R
|
R

D
x
E

G

G

E

E

Q

Q

V

T

P

P

G

G

C

V

Q

V

L

F

N

T

T

T

V

D

E

Q

K

R

I

V

Q

E

V

I

A

A

K

K

A

A

L

L

E

S

A

D

L

I

G

G

V

V

D

Q

V

M

I

I

E

E

A

A

G

G

F

H

P

P

I

A

S
x
V

S

S

P

P

G

D

D

I

F

Y

N

E

S

G

V

I

I

R

E

R

I

I

S

I

K

K

-

L

-

K

-

R

-

E

A

G

V

V

T

I

W

K

P

S

T

E

I

I

C

V

A

A

L

H

T

S

R
|
R

A
|
A

V

V

Q

K

K

R

D

D

I

I

D

E

V

V

A

G

V

A

D

E

A

I

L

-

K

-

F

-

A

-

K

E

H

A

K

D

R

R

I

I

H

A

T

I

G
x
F

I

Y

G

G

T

I

S

S

D

D

S

T

H

H

I

L

K

K

Y

A

K

K

F
x
H

N

H

S

T

N

T

R

R

E

D

E

E

I

A

I

L

E

R

R

S

A

I

V

A

A

E

A

T

V

V

K

S

Y

Y

A

A

R

K

R

S

F

H

V

G

D

V

D

K

V

V

E
x
R

F

F

Y
x
T

A

A

E
|
E

D

D

A

A

G

T

R

R

T

A

D

D

N

Y

E

Q

Y

Y

L

L

A

L

R

E

V

V

V

I

E

K

A

T

V

V

I

R

K

D

A

A

G

G

A

A

T

D

V

R

V

V

N
x
S

I

I

P

A

D

D

T
|
T

T

V

G

G

Y

V

C

L

L

Y

P

P

S

S

E

R

Y

T

G

R

A

E

K

L

I

F

K

K

Y

D

L

L

I

T

D

S

H

R

V

F

D

P

G

D

I

I

D

E

N

-

A

-

I

-

L

F

S

D

T

I

H
|
H

C

A

H
|
H

N

N

D

D

L

L

G

G

M

M

A

A

T

V

A

A

N

N

T

V

I

L

A

A

G

A

V

A

L

E

N

G

G

G

A

A

R

T

Q

I

V

I

E

H

V

T

T

T

I

L

N

N

G

G

I

L

G

G

E

E

R

R

A

V

G

G

N

I

T

A

A

P

L

L

E

Q

E

V

I

V

A

A

M

A

I

A

I

L

K

K

S

Y

H

H

H

F

E

G

I

I

D

E

I

V

Q

-

T

-

N

-

I

V

N

D

T

L

Q

K

K

K

I

L

Y

S

P

E

T

V

S

A

R

S

M

L

V

V

S

E

S

K

L

Y

M

S

N

G

M

I

P

A

V

L

Q

P

P

P

N

N

K

F

A

P

I

I

V

T

G

G

R

D

N

Y

A

A

F

F

A

V

H

H

S

K

S

A

G

G

I

V

H

H

Q

V

D

A

G

G

V

V

L

L

K

N

N

D

V

P

E

K

T

T

Y

Y

E

E

I

F

I

L

D

P

P

P

H

E

D

T

V

F

G

G

I

-

D

R

D

S

N

R

S

D

I

Y

V

V

L

I

T

D

A

K

R

Y

S

T

G

G

R

K

A

H

A

A

L

V

K

K

N

D

R

R

L

F

S

D

L

R

L

L

G

G

V

V

N

K

L

L

D

T

Q

D

E

S

K

E

L

I

D

D

K

Q

V

V

binding 2-oxoglutaric acid: R30 (= R13), R154 (≠ E137), T156 (≠ Y139), E158 (= E141), S184 (≠ N167), T188 (= T171), H216 (= H202), H218 (= H204)
binding coenzyme a: V67 (≠ S50), R96 (= R75), A97 (= A76), F116 (≠ G99), H128 (≠ F111), E158 (= E141)
binding zinc ion: E31 (≠ D14), H216 (= H202), H218 (= H204)

4ov4A Isopropylmalate synthase binding with ketoisovalerate (see paper)
34% identity, 75% coverage: 5:378/498 of query aligns to 4:378/379 of 4ov4A

query
sites
4ov4A

L

I

F

H

I

I

F

Q

D

D

T

V

T

T

L

L

R
|
R

D
|
D

G

G

E

N

Q
|
Q

V

A

P

L

G

K

C

R

Q

P

L

W

N

T

T

I

V

D

E

E

K

K

I

I

Q

E

V

V

A

F

K

D

A

L

L

L

E

V

A

E

L

L

G

N

V

V

D

D

V

G

I

I

E

E

A

V

G

G

F

F

P

P

I

S

S

S

S

N

P

E

G

T

D

E

F

F

N

H

S

T

V

C

I

Q

E

V

I

L

S

S

K

K

-

R

A

A

V

P

T

K

W

G

P

K

T

P

I

I

C

A

A

A

L

L

T

S

R

R

A

A

V

N

Q

Q

K

N

D

E

I

I

D

A

V

V

A

T

V

W

D

E

A

A

L

I

K

Q

F

K

A

A

K

D

H

C

K

P

R

R

I

M

H

H

T

I

G

V

I

Y

G

P

T

V

S

S

D

D

S

F

H

S

I

I

K

K

Y

H

K

V

F

L

N

K

S

I

N

S

R

E

E

K

E

E

I

V

I

L

E

Q

R

K

A

I

V

R

A

N

A

S

V

I

K

S

Y

F

A

A

R

R

R

S

F

I

V

V

D

G

-

P

-

G

-

I

D

E

V

I

E

Q

F

F

Y

S

A

G

E

E

D

H

A

F

G

G

R

D

T

A

D

I

N

-

E

E

Y

N

L

F

A

A

R

F

V

T

V

K

E

E

A

A

V

F

-

L

-

T

-

A

I

I

K

E

A

A

G

G

A

A

T

N

V

I

N

N

I

L

P
|
P

D

N

T
|
T

T

V

G

E

Y

R

C

Y

L

R

P

P

S

M

E

V

Y

F

G

V

A

N

K

M

I

V

K

K

Y

E

L

I

I

K

D

D

H

V

V

V

D

K

G

-

I

-

D

D

N

K

A

A

I

I

L

I

S

S

T

I

H
|
H

C

T

H
|
H

N

N

D

D

L

L

G

G

M

M

A

A

T

T

A

A

N

T

T

S

I

V

A

E

G

S

V

V

L

Y

N

V

G

G

A

A

R

E

Q

Q

V

I

E

E

V

V

T

A

I

L

N

N

G

G

I

L

G

G

E

E

R

R

A

A

G

G

N

N

T

T

A

N

L

L

E

Y

E

E

I

T

A

A

M

I

I

A

I

L

K

H

S

Q

H

N

H

G

E

E

I

-

D

-

I

-

Q

N

T

L

N

N

I

I

N

N

T

F

Q

Q

K

R

I

I

Y

Y

P

P

T

T

S

A

R

K

M

R

V

I

S

S

S

E

L

L

M

T

N

G

M

I

P

P

V

I

Q

G

P

E

N

K

K

T

A

P

I

I

V

I

G

G

R

E

N

D

A

I

F

F

A

S

H

H

S

R

S

S

G

G

I

I

H

H

Q

Q

D

H

G

-

V

-

L

-

K

Q

N

S

V

K

E

G

T

A

Y

Y

E

R

I

T

I

F

D

S

P

P

H

E

D

F

V

V

G

G

-

R

I

M

D

D

D

K

N

E

S

T

I

I

V

S

L

F

T

T

A

N

R

Q

S

S

G

G

R

H

A

K

A

A

L

I

K

E

N

F

R

L

L

L

S

H

L

Q

L

R

G

G

V

I

N

Q

L

V

D

S

Q

K

E

E

K

G

L

I

D

H

K

H

V

L

Y

F

E

S

E

L

F

A

L

K

K

S

L

I

A

S

D

S

K

R

K

E

-

N

K

R

D

E

I

I

N

T

D

E

D

A

D

E

V

L

L

V

V

A

L

L

A

S

active site: Q16 (= Q17)
binding 3-methyl-2-oxobutanoic acid: R12 (= R13), P174 (= P169), T176 (= T171), H205 (= H202), H207 (= H204)
binding zinc ion: D13 (= D14), H205 (= H202), H207 (= H204)

4ov9A Structure of isopropylmalate synthase binding with alpha- isopropylmalate (see paper)
34% identity, 75% coverage: 5:378/498 of query aligns to 4:380/380 of 4ov9A

query
sites
4ov9A

L

I

F

H

I

I

F

Q

D

D

T

V

T

T

L

L

R
|
R

D
|
D

G

G

E

N

Q
|
Q

V

A

P

L

G

K

C

R

Q

P

L

W

N

T

T

I

V

D

E

E

K

K

I

I

Q

E

V

V

A

F

K

D

A

L

L

L

E

V

A

E

L

L

G

N

V

V

D

D

V

G

I

I

E

E

A

V

G

G

F

F

P

P

I

S

S

S

S

N

P

E

G

T

D

E

F

F

N

H

S

T

V

C

I

Q

E

V

I

L

S

S

K

K

-

R

A

A

V

P

T

K

W

G

P

K

T

P

I

I

C

A

A

A

L
|
L

T

S

R

R

A

A

V

N

Q

Q

K

N

D

E

I

I

D

A

V

V

A

T

V

W

D

E

A

A

L

I

K

Q

F

K

A

A

K

D

H

C

K

P

R

R

I

M

H

H

T

I

G

V

I

Y

G

P

T

V

S

S

D

D

S

F

H

S

I

I

K

K

Y

H

K

V

F

L

N

K

S

I

N

S

R

E

E

K

E

E

I

V

I

L

E

Q

R

K

A

I

V

R

A

N

A

S

V

I

K

S

Y

F

A

A

R

R

R

S

F

I

V

V

D

G

-

P

-

G

-

I

D

E

V

I

E

Q

F

F

Y

S

A

G

E

E

D

H

A

F

G

G

R

D

T

A

D

I

N

-

E

E

Y

N

L

F

A

A

R

F

V

T

V

K

E

E

A

A

V

F

-

L

-

T

-

A

I

I

K

E

A

A

G

G

A

A

T

N

V

I

N

N

I

L

P

P

D

N

T
|
T

T

V

G

E

Y

R

C

Y

L

R

P

P

S

M

E

V

Y

F

G

V

A

N

K

M

I

V

K

K

Y

E

L

I

I

K

D

D

H

V

V

V

D

K

G

-

I

-

D

D

N

K

A

A

I

I

L

I

S

S

T

I

H
|
H

C

T

H
|
H

N

N

D

D

L

L

G

G

M

M

A

A

T

T

A

A

N

T

T

S

I

V

A

E

G

S

V

V

L

Y

N

V

G

G

A

A

R

E

Q

Q

V

I

E

E

V

V

T

A

I

L

N

N

G

G

I

L

G

G

E

E

R

R

A

A

G

G

N

N

T

T

A

N

L

L

E

Y

E

E

I

T

A

A

M

I

I

A

I

L

K

H

S

Q

H

N

H

G

E

E

I

-

D

-

I

-

Q

N

T

L

N

N

I

I

N

N

T

F

Q

Q

K

R

I

I

Y

Y

P

P

T

T

S

A

R

K

M

R

V

I

S

S

S

E

L

L

M

T

N

G

M

I

P

P

V

I

Q

G

P

E

N

K

K

T

A

P

I

I

V

I

G

G

R

E

N

D

A

I

F

F

A

S

H

H

S

R

S

S

G

G

I

I

H

H

Q

Q

D

T

G

-

V

L

L

H

K

Q

N

S

V

K

E

G

T

A

Y

Y

E

R

I

T

I

F

D

S

P

P

H

E

D

F

V

V

G

G

-

R

I

M

D

D

D

K

N

E

S

T

I

I

V

S

L

F

T

T

A

N

R

Q

S

S

G

G

R

H

A

K

A

A

L

I

K

E

N

F

R

L

L

L

S

H

L

Q

L

R

G

G

V

I

N

Q

L

V

D

S

Q

K

E

E

K

G

L

I

D

H

K

H

V

L

Y

F

E

S

E

L

F

A

L

K

K

S

L

I

A

S

D

S

K

R

K

E

-

N

K

R

D

E

I

I

N

T

D

E

D

A

D

E

V

L

L

V

V

A

L

L

A

S

active site: Q16 (= Q17)
binding (2S)-2-hydroxy-2-(propan-2-yl)butanedioic acid: R12 (= R13), L73 (= L73), T176 (= T171), H205 (= H202), H207 (= H204)
binding zinc ion: D13 (= D14), H205 (= H202), H207 (= H204)

Q8F3Q1 (R)-citramalate synthase CimA; LiCMS; EC 2.3.3.21 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see 2 papers)
29% identity, 88% coverage: 1:439/498 of query aligns to 4:443/516 of Q8F3Q1

query
sites
Q8F3Q1

M

V

D

E

N

T

R

R

L

L

F

E

I

I

F

L

D

D

T

V

T

T

L

L

R
|
R

D
|
D

G

G

E

E

Q

Q

V

T

P

R

G

G

C

V

Q

S

L

F

N

S

T

T

V

S

E

E

K

K

I

L

Q

N

V

I

A

A

K

K

-

F

A

L

L

L

E

Q

A

K

L

L

G

N

V

V

D

D

V

R

I

V

E

E

A

I

G

A

F

S

P

A

I

R

S

V

S

S

P

K

G

G

D

E

F

L

N

E

S

T

V

V

I

-

E

-

I

-

S

Q

K

K

A

I

V

M

T

E

W

W

P

A

T

A

I

T

C

E

A

Q

L

L

T

T

R

E

A

R

V

I

Q

E

-

I

-
x
L

-

G

-
x
F

K

V

D

D

I

G

D

N

V

K

A

T

V

V

D

D

A

W

L

I

K

K

F

D

A

S

K

G

H

A

K

K

R

V

I

L

H

N

T

L

G
x
L

I

T

G

K

T

G

S

S

D

L

S

H

H

H

I

L

K

E

Y

K

K

Q

F

L

N

G

S

K

N

T

R

P

E

K

E

E

I

F

E

T

R

D

A

V

V

S

A

F

A

V

V

I

K

E

Y

Y

A

A

R

I

R

K

F

S

V

G

D

L

D

K

V

I

E

N

F

V

Y
|
Y

A

L

E
|
E

D

D

A

W

G

S

-

N

-

G

-

F

R

R

T

N

D

S

N

P

E

D

Y

Y

L

V

A

K

R

S

V

L

V

V

E

E

A

H

V

L

I

S

K

K

A

E

G

H

A

I

T

E

V

R

V

I

N

F

I

L

P

P

D

D

T
|
T

T

L

G

G

Y

V

C

L

L

S

P

P

S

E

E

E

Y

T

G

F

A

Q

K

G

I

V

K

D

Y

S

L

L

I

I

D

Q

H

K

V

Y

D

P

G

D

I

I

D

H

N

-

A

-

I

-

L

F

S

E

T

F

H

H

C

G

H

H

N

N

D

D

L

Y

G

D

M

L

A

S

T

V

A

A

N

N

T

S

I

L

A

Q

G

A

V

I

L

R

N

A

G

G

A

V

R

K

Q

G

V

L

E

H

V

A

T

S

I

I

N

N

G

G

I

L

G

G

E

E

R

R

A

A

G

G

N

N

T

T

A

P

L

L

E

E

E

-

I

-

A

A

M

L

I

V

I

T

K

T

S

I

H

H

H

D

E

K

I

S

D

N

I

S

Q

K

T

T

N

N

I

I

N

N

T

E

Q

I

K

A

I

I

Y

T

P

E

T

A

S

S

R

R

M

L

V

V

S

E

S

V

L

F

M

S

N

G

M

K

P

R

V

I

Q

S

P

A

N

N

K

R

A

P

I

I

V

V

G

G

R

E

N

D

A

V

F

F

A

T

H

Q

S

T

S

A

G

G

I

V

H
|
H

Q

A

D
|
D

G

G

V

D

L

K

K

K

N

G

V
x
N

E
x
L

T
x
Y

Y

A

E

N

I

P

I

I

D

L

P

P

H

E

D

R

V

F

G

G

I

-

D

R

D

K

N

R

S

S

I

Y

V

A

L

L

T

G

A

K

R

L

S

A

G

G

R

K

A

A

A

S

L

I

K

S

N

E

R

N

L

V

S

K

L

Q

L

L

G

G

V

M

N

V

L

L

D

S

Q

E

E

V

K

V

L

L

D

Q

K

K

V

V

Y

L

E

E

E

R

F

V

L

I

K

E

L

L

A

G

D

D

K

Q

K

N

K

K

D

L

I

V

N

T

D

P

D

E

D

D

V

L

L

P

V

F

L

I

A

I

G

A

-

D

-

V

A

S

D

G

R

R

S

T

Q

G

N

E

H

K

R

V

I

L

K

T

L

I

E

K

Y

S

L

C

Q

N

V

I

T

H

S

S

G

G

V

I

G

G

V

I

R

R

S

P

V

H

A

A

S

Q

L

I

G

E

L

L

N

E

I

Y

S

Q

G

G

E

K

K

I

F

H

E

K

A

E

C

I

A

S

S

E

G

G

N

D

G

G

P

G

V
x
Y

D
|
D

A

A

A

F

I

M

K

N

A

A

L

L

K

T

K

K

I

I

V

T

D

N

R

R

R16 (= R13) mutation R->K,Q: Loss of activity.
RD 16:17 (= RD 13:14) binding pyruvate
D17 (= D14) mutation to A: 34-fold increase in Km for pyruvate and 315-fold decrease in kcat.; mutation to N: 4.4-fold increase in Km for pyruvate and 480-fold decrease in kcat.
L81 (vs. gap) mutation to A: 4.7-fold increase in Km for pyruvate and 15.7-fold decrease in kcat.; mutation to V: 3.3-fold increase in Km for pyruvate and 10.1-fold decrease in kcat.
F83 (vs. gap) mutation to A: 5-fold increase in Km for acetyl-CoA and 120-fold decrease in kcat.
L104 (≠ G99) mutation to V: 1.8-fold increase in Km for pyruvate and 3.4-fold decrease in kcat.
Y144 (= Y139) binding pyruvate; mutation to L: 259-fold increase in Km for pyruvate and 76-fold decrease in kcat.; mutation to V: 114-fold increase in Km for pyruvate and 5.3-fold decrease in kcat.
E146 (= E141) mutation E->D,Q: Minor effects on the binding of acetyl-CoA, but causes a strong decrease in kcat.
T179 (= T171) binding pyruvate; mutation to A: 16.4-fold increase in Km for pyruvate and 186-fold decrease in kcat.
H302 (= H299) mutation H->A,N: Loss of activity.
D304 (= D301) mutation to A: 5.2-fold increase in Km for acetyl-CoA and 16.6-fold decrease in kcat.
N310 (≠ V307) mutation to A: 2.2-fold increase in Km for acetyl-CoA and 1.7-fold decrease in kcat.
L311 (≠ E308) mutation to A: 8-fold increase in Km for acetyl-CoA and 6-fold decrease in kcat.
Y312 (≠ T309) mutation to A: Loss of activity.
Y430 (≠ V426) mutation to L: No change in Km for acetyl-CoA and 2.3-fold decrease in kcat. Severely impairs inhibition by isoleucine.
D431 (= D427) mutation to A: 1.8-fold decrease in Km for acetyl-CoA and 5-fold decrease in kcat.

Sites not aligning to the query:

451 L→V: 1.5-fold increase in Km for acetyl-CoA and 4.3 decrease in kcat.
454 Y→A: 1.4 decrease in Km for acetyl-CoA and 17-fold decrease in kcat. Still inhibited by isoleucine and weakly inhibited by leucine.
458 I→A: 1.3-fold decrease in Km for acetyl-CoA and 14-fold decrease in kcat. Abolishes inhibition by isoleucine.
464 T→A: 1.8-fold decrease in Km for acetyl-CoA and 4.3-fold decrease in kcat.
468 V→A: No change in Km for acetyl-CoA and 2-fold decrease in kcat. Increases inhibition by isoleucine and leucine becomes an effective inhibitor.
493 P→A: 1.5-fold decrease in Km for acetyl-CoA and 2.6-fold decrease in kcat.
495 Q→A: 1.6-fold decrease in Km for acetyl-CoA and 2.8-fold decrease in kcat.

Q9Y823 Homocitrate synthase, mitochondrial; HCS; EC 2.3.3.14 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
31% identity, 78% coverage: 3:391/498 of query aligns to 33:415/418 of Q9Y823

query
sites
Q9Y823

N

N

R

N

L

F

F

S

I

I

F

I

D

E

T

S

T

T

L

L

R
|
R

D
x
E

G

G

E

E

Q
|
Q

V

F

P

A

G

N

C

A

Q

F

L

F

N

D

T

T

V

E

E

K

K

K

I

I

Q

Q

V

I

A

A

K

K

A

A

L

L

E

D

A

N

L

F

G

G

V

V

D

D

V

Y

I

I

E
|
E

A

L

G

T

F

S

P

P

I

V

S

A

S

S

P

E

G

Q

D

S

F

R

N

Q

S

D

V

C

I

E

E

A

I

I

S

C

K

K

A

L

V

G

T

L

W

K

P

C

T

K

I

I

C

L

A

T

L
x
H

T

I

R

R

A

C

V

H

Q

M

K

D

D

D

I

A

D

R

V

V

A

A

V

V

D

E

A

T

L

-

K

-

F

-

A

-

K

G

H

V

K

D

R

G

I

V

H
x
D

T

V

G

V

I

I

G

G

T

T

S

S

D

Q

S

Y

H

L

I

R

K

K

Y

Y

K

S

F

H

N

G

S

K

N

D

R

M

E

T

E

Y

I

I

E

D

R

S

A

A

V

T

A

E

A

V

V

I

K

N

Y

F

A

V

R

K

R

S

F

K

V

G

D

I

D

E

V

V

E
x
R

F

F

Y
x
S

A

S

E
|
E

D

D

A

S

G

F

R

R

T

S

D

D

N

L

E

V

Y

D

L

L

A

L

R

S

V

L

V

Y

E

K

A

A

V

V

I

D

K

K

A

I

G

G

A

V

T

N

V

R

V

V

N

G

I

I

P

A

D

D

T
|
T

T

V

G

G

Y

C

C

A

L

T

P

P

S

R

E

Q

Y

-

G

-

A

-

K

-

I

V

K

Y

Y

D

L

L

I

I

D

R

H

T

V

L

D

R

G

G

I

V

D

V

N

S

A

C

I

D

L

I

S
x
E

T

C

H
|
H

C

F

H
|
H

N

N

D

D

L

T

G

G

M

M

A

A

T

I

A

A

N

N

T

A

I

Y

A

C

G

A

V

L

L

E

N

A

G

G

A

A

R

T

Q

H

V

I

E

D

V

T

T

S

I

I

N

L

G

G

I

I

G

G

E

E

R

R

A

N

G

G

N

I

T

T

A

P

L

L

E

G

E

A

I

L

A

L

-

A

-

R

M

M

I

Y

I

V

K

T

S

D

H

R

H

E

E

Y

I

I

D

T

I

H

Q

K

T

Y

N

K

I

L

N

N

T

-

Q

-

K

Q

I

L

Y
x
R

P

E

T

L

S

E

R

N

M

L

V

V

S

A

S

D

L

A

M

V

N

E

M

V

P

Q

V

I

Q

P

P

F

N

N

K

N

A

Y

I

I

V

T

G

G

R

M

N

C

A

A

F

F

A

T

H

H

S

K

S

A

G

G

I

I

H

H

Q

A

D

K

G

A

V

I

L

L

K

A

N

N

V

P

E

S

T

T

Y
|
Y

E

E

I

I

I

L

D

K

P

P

H

E

D

D

V

F

G

G

I

M

D

S

D

R

N

Y

S

V

I

H

V

V

L

G

T

S

A

R

R

L

S

T

G

G

R

W

A

N

A

A

L

I

K

K

N

S

R

R

L

A

S

E

L
x
Q

L

L

G

N

V

L

N

H

L

L

D

T

Q

D

E

A

K

Q

L

A

D

K

K

E

V

L

Y

T

E

V

E

R

F

I

L

K

K

K

L

L

A

A

D

D

K

V

K

R

K

T

D

L

I

A

N

M

D

D

-

D

-

V

D

D

D

R

V

V

L

L

V

R

L

E

A

Y

G

H

A

A

D

D

R

L

S

S

Q

D

N

A

H

D

R

R

I

I

K

T

L

K

E

E

R43 (= R13) binding 2-oxoglutarate; mutation R->A,K,Q: Abolishes the catalytic activity.
E44 (≠ D14) binding 2-oxoglutarate; binding L-lysine; binding Zn(2+)
Q47 (= Q17) mutation to A: Abolishes the catalytic activity.
E74 (= E44) mutation to A: Abolishes the catalytic activity.; mutation to Q: Results in a moderate decrease in the turnover number and a slight increase in the Km value for each substrate.
H103 (≠ L73) binding 2-oxoglutarate; mutation to A: Substantially impairs catalytic efficiency.
D123 (≠ H97) binding L-lysine; mutation to N: Does not affect the catalytic activity but impairs L-lysine inhibition.
R163 (≠ E137) binding 2-oxoglutarate; mutation R->A,Q: Abolishes the catalytic activity.; mutation to K: Severely diminishes affinity for 2-oxoglutarate and substantially impairs catalytic efficiency.
S165 (≠ Y139) binding 2-oxoglutarate; mutation to A: Results in a moderate decrease in catalytic efficiency.
E167 (= E141) mutation E->A,Q: Abolishes the catalytic activity.
T197 (= T171) binding 2-oxoglutarate; binding L-lysine; mutation to A: Exhibits a 25-fold decrease in catalytic efficiency.; mutation to S: Results in a modest decrease in catalytic efficiency.; mutation to V: Abolishes the catalytic activity.
E222 (≠ S200) mutation to Q: Does not affect the catalytic activity but impairs L-lysine inhibition.
H224 (= H202) binding 2-oxoglutarate; binding Zn(2+)
H226 (= H204) binding 2-oxoglutarate; binding Zn(2+)
R288 (≠ Y266) mutation to K: Does not affect the catalytic activity but impairs L-lysine inhibition.
Y332 (= Y310) mutation to A: Abolishes the catalytic activity.; mutation to F: Results in a decrease in catalytic efficiency.
Q364 (≠ L342) mutation to R: Does not affect the catalytic activity but impairs L-lysine inhibition.

3ivtB Homocitrate synthase lys4 bound to 2-og (see paper)
31% identity, 74% coverage: 3:372/498 of query aligns to 28:389/400 of 3ivtB

query
sites
3ivtB

N

N

R

N

L

F

F

S

I

I

F

I

D

E

T

S

T

T

L

L

R
|
R

D
x
E

G

G

E

E

Q
|
Q

V

F

P

A

G

N

C

A

Q

F

L

F

N

D

T

T

V

E

E

K

K

K

I

I

Q

Q

V

I

A

A

K

K

A

A

L

L

E

D

A

N

L

F

G

G

V

V

D

D

V

Y

I

I

E

E

A

L

G

T

F

S

P

P

I

V

S

A

S

S

P

E

G

Q

D

S

F

R

N

Q

S

D

V

C

I

E

E

A

I

I

S

C

K

K

A

L

V

G

T

L

W

K

P

C

T

K

I

I

C

L

A

T

L
x
H

T

I

R

R

A

C

V

H

Q

M

K

D

D

D

I

A

D

R

V

V

A

A

V

V

D

E

A

T

L

-

K

-

F

-

A

-

K

G

H

V

K

D

R

G

I

V

H

D

T

V

G

V

I

I

G

G

T

T

S

S

D

Q

S

Y

H

L

I

R

K

K

Y

Y

K

S

F

H

N

G

S

K

N

D

R

M

E

T

E

Y

I

I

E

D

R

S

A

A

V

T

A

E

A

V

V

I

K

N

Y

F

A

V

R

K

R

S

F

K

V

G

D

I

D

E

V

V

E
x
R

F

F

Y
x
S

A

S

E

E

D

D

A

S

G

F

R

R

T

S

D

D

N

L

E

V

Y

D

L

L

A

L

R

S

V

L

V

Y

E

K

A

A

V

V

I

D

K

K

A

I

G

G

A

V

T

N

V

R

V

V

N

G

I

I

P

A

D

D

T
|
T

T

V

G

G

Y

C

C

A

L

T

P

P

S

R

E

Q

Y

-

G

-

A

-

K

-

I

V

K

Y

Y

D

L

L

I

I

D

R

H

T

V

L

D

R

G

G

I

V

D

V

N

S

A

C

I

D

L

I

S

E

T

C

H
|
H

C

F

H
|
H

N

N

D

D

L

T

G

G

M

M

A

A

T

I

A

A

N

N

T

A

I

Y

A

C

G

A

V

L

L

E

N

A

G

G

A

A

R

T

Q

H

V

I

E

D

V

T

T

S

I

I

N

L

G

G

I

I

G

G

E

E

R

R

A

N

G

G

N

I

T

T

A

P

L

L

E

G

E

A

I

L

A

L

-

A

-

R

M

M

I

Y

I

V

K

T

S

D

H

R

H

E

E

Y

I

I

D

T

I

H

Q

K

T

Y

N

K

I

L

N

N

T

-

Q

-

K

Q

I

L

Y

R

P

E

T

L

S

E

R

N

M

L

V

V

S

A

S

D

L

A

M

V

N

E

M

V

P

Q

V

I

Q

P

P

F

N

N

K

N

A

Y

I

I

V

T

G

G

R

M

N

C

A

A

F

F

A

T

H

H

S

K

S

A

G

G

I

I

H

H

Q

A

D

K

G

A

V

I

L

L

K

A

N

N

V

P

E

S

T

T

Y

Y

E

E

I

I

I

L

D

K

P

P

H

E

D

D

V

F

G

G

I

M

D

S

D

R

N

Y

S

V

I

H

V

V

L

G

T

S

A

R

R

L

S

T

G

G

R

W

A

N

A

A

L

I

K

K

N

S

R

R

L

A

S

E

L

Q

L

L

G

N

V

L

N

H

L

L

D

T

Q

D

E

A

K

Q

L

A

D

K

K

E

V

L

Y

T

E

V

E

R

F

I

L

K

K

K

L

L

A

A

D

D

K

V

K

R

K

T

D

L

I

A

N

M

D

D

active site: Q42 (= Q17)