SitesBLAST
Comparing 351409 FitnessBrowser__Btheta:351409 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8swtA Structure of bacteroides fragilis pnp bound to transition state analog immucillin h and sulfate (see paper)
97% identity, 100% coverage: 1:269/269 of query aligns to 10:278/278 of 8swtA
- binding 1,4-dideoxy-4-aza-1-(s)-(9-deazahypoxanthin-9-yl)-d-ribitol: H90 (= H81), Y92 (= Y83), A120 (= A111), S121 (= S112), G122 (= G113), F198 (= F189), E199 (= E190), Y204 (= Y195), G216 (= G207), M217 (= M208), D241 (= D232), H253 (= H244)
P46354 Purine nucleoside phosphorylase 1; PNP 1; Inosine phosphorylase; Inosine-guanosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; Pu-NPase I; EC 2.4.2.1 from Bacillus subtilis (strain 168) (see paper)
51% identity, 99% coverage: 1:266/269 of query aligns to 1:267/271 of P46354
- S28 (≠ T28) modified: Phosphoserine
4lnaA Crystal structure of purine nucleoside phosphorylase i from spirosoma linguale dsm 74, nysgrc target 029362
53% identity, 100% coverage: 1:268/269 of query aligns to 2:268/269 of 4lnaA
- active site: T29 (= T28), H79 (= H81), Y81 (= Y83), E82 (= E84), A109 (= A111), M208 (= M208), S209 (= S209), D232 (= D232), C234 (≠ G234), L244 (≠ H244)
- binding adenine: A110 (≠ S112), G111 (= G113), E190 (= E190), M208 (= M208), D232 (= D232)
8swuA Structure of clostridium perfringens pnp bound to transition state analog immucillin h and sulfate (see paper)
51% identity, 98% coverage: 4:266/269 of query aligns to 7:266/269 of 8swuA
- binding 1,4-dideoxy-4-aza-1-(s)-(9-deazahypoxanthin-9-yl)-d-ribitol: Y82 (= Y83), A110 (= A111), A111 (≠ S112), G112 (= G113), Y189 (≠ F189), E190 (= E190), M208 (= M208), N232 (≠ D232), H244 (= H244), V247 (= V247)
4nsnA Crystal structure of purine nucleoside phosphorylase from porphyromonas gingivalis atcc 33277, nysgrc target 030972, orthorhombic symmetry
56% identity, 97% coverage: 6:266/269 of query aligns to 9:270/273 of 4nsnA
- active site: S31 (≠ T28), H62 (= H59), H84 (= H81), Y86 (= Y83), E87 (= E84), A114 (= A111), M211 (= M208), S212 (= S209), N235 (≠ D232), E248 (= E245)
- binding adenine: A114 (= A111), A115 (≠ S112), G116 (= G113), Y192 (≠ F189), E193 (= E190), Y198 (= Y195), G210 (= G207), M211 (= M208), T234 (= T231), N235 (≠ D232)
4m1eB Crystal structure of purine nucleoside phosphorylase i from planctomyces limnophilus dsm 3776, nysgrc target 029364.
47% identity, 99% coverage: 4:268/269 of query aligns to 6:271/273 of 4m1eB
- active site: T30 (= T28), H61 (= H59), H83 (= H81), Y85 (= Y83), E86 (= E84), A113 (= A111), M211 (= M208), S212 (= S209), D235 (= D232), C237 (≠ G234), L247 (≠ H244)
- binding pyridine-2-carboxylic acid: N118 (= N116), Q120 (≠ A118), R169 (≠ D166), H178 (≠ K175), P180 (≠ Q177), P180 (≠ Q177)
- binding adenine: A114 (≠ S112), G115 (= G113), E193 (= E190), V209 (= V206), D235 (= D232)
4ns1A Crystal structure of purine nucleoside phosphorylase from porphyromonas gingivalis atcc 33277, nysgrc target 30972
54% identity, 97% coverage: 6:266/269 of query aligns to 9:265/268 of 4ns1A
- active site: S31 (≠ T28), H79 (= H81), Y81 (= Y83), E82 (= E84), A109 (= A111), M206 (= M208), S207 (= S209), N230 (≠ D232), E243 (= E245)
- binding 2'-deoxyadenosine-5'-monophosphate: A109 (= A111), G111 (= G113), E188 (= E190), Y193 (= Y195), I204 (≠ V206), G205 (= G207), M206 (= M208), T229 (= T231), N230 (≠ D232)
1a9sA Bovine purine nucleoside phosphorylase complexed with inosine and sulfate (see paper)
49% identity, 98% coverage: 3:266/269 of query aligns to 8:279/282 of 1a9sA
- active site: S33 (≠ T28), H64 (= H59), H86 (= H81), Y88 (= Y83), E89 (= E84), A116 (= A111), M219 (= M208), S220 (= S209), N243 (vs. gap), V245 (vs. gap), H257 (= H244)
- binding inosine: Y88 (= Y83), A116 (= A111), G118 (= G113), F200 (= F189), E201 (= E190), M219 (= M208), T242 (= T231), N243 (vs. gap), H257 (= H244), V260 (= V247)
1a9qA Bovine purine nucleoside phosphorylase complexed with inosine (see paper)
49% identity, 98% coverage: 3:266/269 of query aligns to 8:279/282 of 1a9qA
- active site: S33 (≠ T28), H64 (= H59), H86 (= H81), Y88 (= Y83), E89 (= E84), A116 (= A111), M219 (= M208), S220 (= S209), N243 (vs. gap), V245 (vs. gap), H257 (= H244)
- binding hypoxanthine: A117 (≠ S112), G118 (= G113), F200 (= F189), E201 (= E190), M219 (= M208), N243 (vs. gap)
1a9tA Bovine purine nucleoside phosphorylase complexed with 9-deazainosine and phosphate (see paper)
49% identity, 98% coverage: 3:266/269 of query aligns to 8:279/284 of 1a9tA
- active site: S33 (≠ T28), H64 (= H59), H86 (= H81), Y88 (= Y83), E89 (= E84), A116 (= A111), M219 (= M208), S220 (= S209), N243 (vs. gap), V245 (vs. gap), H257 (= H244)
- binding hypoxanthine: A116 (= A111), A117 (≠ S112), G118 (= G113), F200 (= F189), E201 (= E190), V217 (= V206), N243 (vs. gap)
- binding 1-O-phosphono-alpha-D-ribofuranose: S33 (≠ T28), R84 (= R79), H86 (= H81), Y88 (= Y83), N115 (= N110), A116 (= A111), F200 (= F189), M219 (= M208), S220 (= S209), V260 (= V247)
1b8oA Purine nucleoside phosphorylase (see paper)
49% identity, 98% coverage: 3:266/269 of query aligns to 6:277/280 of 1b8oA
- active site: S31 (≠ T28), H62 (= H59), H84 (= H81), Y86 (= Y83), E87 (= E84), A114 (= A111), M217 (= M208), S218 (= S209), N241 (vs. gap), V243 (vs. gap), H255 (= H244)
- binding 1,4-dideoxy-4-aza-1-(s)-(9-deazahypoxanthin-9-yl)-d-ribitol: H84 (= H81), Y86 (= Y83), A114 (= A111), G116 (= G113), F198 (= F189), E199 (= E190), M217 (= M208), N241 (vs. gap), H255 (= H244), V258 (= V247)
- binding phosphate ion: G30 (= G27), S31 (≠ T28), R82 (= R79), H84 (= H81), N113 (= N110), S218 (= S209)
1b8nA Purine nucleoside phosphorylase (see paper)
49% identity, 98% coverage: 3:266/269 of query aligns to 7:278/278 of 1b8nA
- active site: S32 (≠ T28), H63 (= H59), H85 (= H81), Y87 (= Y83), E88 (= E84), A115 (= A111), M218 (= M208), S219 (= S209), N242 (vs. gap), V244 (vs. gap), H256 (= H244)
- binding 1,4-dideoxy-1,4-imino-1-(s)-(9-deazaguanin-9-yl)-d-ribitol: H85 (= H81), Y87 (= Y83), A115 (= A111), G117 (= G113), F199 (= F189), E200 (= E190), V216 (= V206), G217 (= G207), M218 (= M208), N242 (vs. gap), H256 (= H244), V259 (= V247)
1a9pA Bovine purine nucleoside phosphorylase complexed with 9-deazainosine and phosphate (see paper)
49% identity, 98% coverage: 3:266/269 of query aligns to 8:279/289 of 1a9pA
- active site: S33 (≠ T28), H64 (= H59), H86 (= H81), Y88 (= Y83), E89 (= E84), A116 (= A111), M219 (= M208), S220 (= S209), N243 (vs. gap), V245 (vs. gap), H257 (= H244)
- binding 9-deazainosine: H86 (= H81), Y88 (= Y83), A116 (= A111), G118 (= G113), F200 (= F189), E201 (= E190), M219 (= M208), N243 (vs. gap), H257 (= H244), E259 (= E246)
- binding phosphate ion: G32 (= G27), S33 (≠ T28), H64 (= H59), R84 (= R79), H86 (= H81), N115 (= N110), S220 (= S209)
1a9oA Bovine purine nucleoside phosphorylase complexed with phosphate (see paper)
49% identity, 98% coverage: 3:266/269 of query aligns to 8:279/289 of 1a9oA
- active site: S33 (≠ T28), H64 (= H59), H86 (= H81), Y88 (= Y83), E89 (= E84), A116 (= A111), M219 (= M208), S220 (= S209), N243 (vs. gap), V245 (vs. gap), H257 (= H244)
- binding phosphate ion: G32 (= G27), R84 (= R79), N115 (= N110), S220 (= S209)
P55859 Purine nucleoside phosphorylase; PNP; Inosine phosphorylase; Inosine-guanosine phosphorylase; EC 2.4.2.1 from Bos taurus (Bovine) (see paper)
49% identity, 98% coverage: 3:266/269 of query aligns to 8:279/289 of P55859
- S33 (≠ T28) binding
- H64 (= H59) binding
- RFH 84:86 (= RFH 79:81) binding
- Y88 (= Y83) binding
- A116 (= A111) binding
- E201 (= E190) binding
- M219 (= M208) binding
- S220 (= S209) binding
- N243 (vs. gap) binding
- H257 (= H244) binding
1fxuA Purine nucleoside phosphorylase from calf spleen in complex with n(7)- acycloguanosine inhibitor and a phosphate ion (see paper)
48% identity, 98% coverage: 3:266/269 of query aligns to 5:273/278 of 1fxuA
- active site: S30 (≠ T28), H61 (= H59), H83 (= H81), Y85 (= Y83), E86 (= E84), A113 (= A111), M216 (= M208), S217 (= S209), N240 (≠ D232), V242 (≠ I239), H251 (= H244)
- binding 2-amino-7-[2-(2-hydroxy-1-hydroxymethyl-ethylamino)-ethyl]-1,7-dihydro-purin-6-one: Y85 (= Y83), A113 (= A111), A114 (≠ S112), G115 (= G113), F197 (= F189), E198 (= E190), M216 (= M208), T239 (= T231), N240 (≠ D232), H251 (= H244), V254 (= V247)
- binding phosphate ion: G29 (= G27), S30 (≠ T28), R81 (= R79), H83 (= H81), N112 (= N110), S217 (= S209)
1lv8A Crystal structure of calf spleen purine nucleoside phosphorylase in a new space group with full trimer in the asymmetric unit (see paper)
52% identity, 86% coverage: 3:232/269 of query aligns to 6:241/265 of 1lv8A
- active site: S31 (≠ T28), H62 (= H59), Y86 (= Y83), A114 (= A111), M217 (= M208), S218 (= S209)
- binding 2,6-diamino-(s)-9-[2-(phosphonomethoxy)propyl]purine: G30 (= G27), S31 (≠ T28), H84 (= H81), N113 (= N110), A114 (= A111), A115 (≠ S112), G116 (= G113), F198 (= F189), E199 (= E190), M217 (= M208), S218 (= S209), N241 (≠ D232)
- binding calcium ion: S49 (= S46), E56 (≠ V53)
3inyA Crystal structure of human purine nucleoside phosphorylase in complex with 7-deazaguanine (see paper)
48% identity, 95% coverage: 3:258/269 of query aligns to 7:270/288 of 3inyA
- active site: S32 (≠ T28), H63 (= H59), H85 (= H81), Y87 (= Y83), E88 (= E84), A115 (= A111), M218 (= M208), S219 (= S209), N242 (≠ D232), V244 (≠ G234), H256 (= H244)
- binding 7-deazaguanine: A115 (= A111), A116 (≠ S112), G117 (= G113), F199 (= F189), E200 (= E190), V216 (= V206), T241 (= T231), N242 (≠ D232)
3d1vA Crystal structure of human pnp complexed with 2-mercapto(3h) quinazolinone (see paper)
48% identity, 95% coverage: 3:258/269 of query aligns to 7:270/288 of 3d1vA
- active site: S32 (≠ T28), H63 (= H59), H85 (= H81), Y87 (= Y83), E88 (= E84), A115 (= A111), M218 (= M208), S219 (= S209), N242 (≠ D232), V244 (≠ G234), H256 (= H244)
- binding 2-mercapto(3H)quinazolinone: A115 (= A111), G117 (= G113), F199 (= F189), E200 (= E190), V216 (= V206), T241 (= T231), N242 (≠ D232)
1yryE Crystal structure of human pnp complexed with mesg (see paper)
48% identity, 95% coverage: 3:258/269 of query aligns to 7:270/288 of 1yryE
- active site: S32 (≠ T28), H63 (= H59), H85 (= H81), Y87 (= Y83), E88 (= E84), A115 (= A111), M218 (= M208), S219 (= S209), N242 (≠ D232), H256 (= H244)
- binding 7-methyl-6-thio-guanosine: Y87 (= Y83), A115 (= A111), A116 (≠ S112), G117 (= G113), E200 (= E190), V216 (= V206), G217 (= G207), M218 (= M208), T241 (= T231), N242 (≠ D232), H256 (= H244)
Query Sequence
>351409 FitnessBrowser__Btheta:351409
MLEKIQETAAFLKGKMHTSPETAIILGTGLGSLADEITEKYEIKYSDIPNFPVSTVEGHS
GKLIFGKLGNKDIMAMQGRFHYYEGYSMKEVTFPVRVMRELGIKTLFVSNASGGTNEAFE
IGDLMIITDHINYFPEHPLRGKNIPYGPRFPDMSEAYDKELIRKADEIAQEKGIKVQHGI
YIGTQGPTFETPAEYKLFHILGADAVGMSTVPEVIVANHCGIKVFGISVITDLGVEGKIV
EVSHEEVQKAADAAQPKMTTIMRELINRA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory