SitesBLAST
Comparing 351600 FitnessBrowser__Btheta:351600 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P19414 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
57% identity, 98% coverage: 15:745/747 of query aligns to 41:770/778 of P19414
- R604 (= R579) mutation to K: Strongly diminishes the catalytic activity towards both known substrates, aconitate and homoaconitate.
Sites not aligning to the query:
- 1:16 modified: transit peptide, Mitochondrion
P20004 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Bos taurus (Bovine) (see 2 papers)
58% identity, 98% coverage: 12:745/747 of query aligns to 45:773/780 of P20004
- Q99 (= Q67) binding substrate
- DSH 192:194 (= DSH 160:162) binding substrate
- C385 (= C359) binding [4Fe-4S] cluster
- C448 (= C422) binding [4Fe-4S] cluster
- C451 (= C425) binding [4Fe-4S] cluster
- R474 (= R448) binding substrate
- R479 (= R453) binding substrate
- R607 (= R579) binding substrate
- SR 670:671 (= SR 642:643) binding substrate
8acnA Crystal structures of aconitase with isocitrate and nitroisocitrate bound (see paper)
57% identity, 98% coverage: 12:745/747 of query aligns to 17:745/753 of 8acnA
- active site: D99 (= D95), H100 (= H96), D164 (= D160), R446 (= R448), S641 (= S641), R643 (= R643)
- binding nitroisocitric acid: Q71 (= Q67), T74 (= T70), H100 (= H96), D164 (= D160), S165 (= S161), R446 (= R448), R451 (= R453), R579 (= R579), S641 (= S641), S642 (= S642), R643 (= R643)
- binding iron/sulfur cluster: H100 (= H96), D164 (= D160), H166 (= H162), S356 (= S358), C357 (= C359), C420 (= C422), C423 (= C425), I424 (= I426)
1fghA Complex with 4-hydroxy-trans-aconitate (see paper)
57% identity, 98% coverage: 12:745/747 of query aligns to 17:745/753 of 1fghA
- active site: D99 (= D95), H100 (= H96), D164 (= D160), R446 (= R448), S641 (= S641), R643 (= R643)
- binding 4-hydroxy-aconitate ion: Q71 (= Q67), T74 (= T70), H100 (= H96), D164 (= D160), S165 (= S161), R446 (= R448), R451 (= R453), R579 (= R579), S641 (= S641), S642 (= S642), R643 (= R643)
- binding iron/sulfur cluster: H100 (= H96), D164 (= D160), H166 (= H162), S356 (= S358), C357 (= C359), C420 (= C422), C423 (= C425), I424 (= I426), R451 (= R453)
1amjA Steric and conformational features of the aconitase mechanism (see paper)
57% identity, 98% coverage: 12:745/747 of query aligns to 17:745/753 of 1amjA
- active site: D99 (= D95), H100 (= H96), D164 (= D160), R446 (= R448), S641 (= S641), R643 (= R643)
- binding iron/sulfur cluster: I144 (= I140), H166 (= H162), C357 (= C359), C420 (= C422), C423 (= C425)
- binding sulfate ion: Q71 (= Q67), R579 (= R579), R643 (= R643)
1amiA Steric and conformational features of the aconitase mechanism (see paper)
57% identity, 98% coverage: 12:745/747 of query aligns to 17:745/753 of 1amiA
- active site: D99 (= D95), H100 (= H96), D164 (= D160), R446 (= R448), S641 (= S641), R643 (= R643)
- binding alpha-methylisocitric acid: Q71 (= Q67), T74 (= T70), H100 (= H96), D164 (= D160), S165 (= S161), R446 (= R448), R451 (= R453), R579 (= R579), S641 (= S641), S642 (= S642), R643 (= R643)
- binding iron/sulfur cluster: H100 (= H96), I144 (= I140), D164 (= D160), H166 (= H162), S356 (= S358), C357 (= C359), C420 (= C422), C423 (= C425), N445 (= N447)
1acoA Crystal structure of aconitase with transaconitate bound (see paper)
57% identity, 98% coverage: 12:745/747 of query aligns to 17:745/753 of 1acoA