SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 351604 FitnessBrowser__Btheta:351604 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

P9WKJ3 Putative acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
30% identity, 86% coverage: 1:161/187 of query aligns to 1:158/168 of P9WKJ3

query
sites
P9WKJ3

M

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K44 (= K45) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

A0QUX7 Acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
30% identity, 86% coverage: 1:161/187 of query aligns to 1:160/170 of A0QUX7

query
sites
A0QUX7

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K46 (= K45) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Q9FFF4 Acetolactate synthase small subunit 2, chloroplastic; ALS-interacting protein 3; Acetohydroxy-acid synthase small subunit 2; Protein valine-tolerant 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 82% coverage: 7:160/187 of query aligns to 308:459/477 of Q9FFF4

query
sites
Q9FFF4

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R453 (= R154) mutation to Q: In vat1-3; resistance to valine inhibition.

Sites not aligning to the query:

88 G→D: Resistance to valine inhibition.
119 T→I: In vat1-1; resistance to valine inhibition.

Q93YZ7 Acetolactate synthase small subunit 1, chloroplastic; ALS-interacting protein 1; Acetohydroxyacid synthase small subunit 1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
27% identity, 82% coverage: 7:160/187 of query aligns to 320:471/491 of Q93YZ7

query
sites
Q93YZ7

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D328 (≠ N15) binding L-valine
G331 (= G18) mutation to D: Decreased feedback inhibition by leucine and valine/isoleucine.
V332 (≠ L19) binding L-valine
L333 (= L20) binding L-valine
F340 (= F27) mutation to A: Decreased feedback inhibition by valine/isoleucine.
Y345 (≠ I32) mutation to A: No effect.
N346 (= N33) binding L-valine; mutation N->A,H: Decreased feedback inhibition by leucine and increased feedback inhibition by valine/isoleucine.
I347 (= I34) binding L-valine

Sites not aligning to the query:

95 binding L-valine
98 G→E: Increased feedback inhibition by valine/isoleucine.
99 binding L-valine
100 binding L-valine
107 F→A: Decreased feedback inhibition by leucine and valine/isoleucine.
112 Y→A: Increased feedback inhibition by leucine.
113 binding L-valine; N→A: Decreased feedback inhibition by valine/isoleucine.
114 binding L-valine

6vz8G Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
28% identity, 82% coverage: 7:160/187 of query aligns to 4:155/159 of 6vz8G

query
sites
6vz8G

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binding valine: D12 (≠ N15), G15 (= G18), V16 (≠ L19), L17 (= L20), N30 (= N33), I31 (= I34), V36 (= V39)

6vz8F Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
24% identity, 82% coverage: 7:160/187 of query aligns to 5:154/159 of 6vz8F

query
sites
6vz8F

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binding valine: D13 (≠ N15), E14 (≠ I16), G16 (= G18), M17 (≠ L19), I18 (≠ L20), N31 (= N33), I32 (= I34)

6wo1B Hybrid acetohydroxyacid synthase complex structure with cryptococcus neoformans ahas catalytic subunit and saccharomyces cerevisiae ahas regulatory subunit (see paper)
23% identity, 79% coverage: 14:160/187 of query aligns to 12:193/197 of 6wo1B

query
sites
6wo1B

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binding valine: E14 (≠ I16), G16 (= G18), L18 (= L20)

6u9dL Saccharomyces cerevisiae acetohydroxyacid synthase (see paper)
29% identity, 44% coverage: 14:96/187 of query aligns to 45:127/255 of 6u9dL

query
sites
6u9dL

E

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Sites not aligning to the query:

binding adenosine-5'-triphosphate: 191, 192, 197, 198, 199, 211, 214, 214, 240

O60086 Probable acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
28% identity, 44% coverage: 14:96/187 of query aligns to 78:160/289 of O60086

query
sites
O60086

E

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Sites not aligning to the query:

34 modified: Phosphoserine

Query Sequence

>351604 FitnessBrowser__Btheta:351604
MSDKTLYTIIVHSENIAGLLNQVTAVFTRRQINIESLNVSASSIKGVHKYTITAWTDKDT
IEKVVKQIEKKIDVIQAHYFTEDEIYFHEIALYKVSTPAFQETPEASKVIRRYNARIVEV
NPVFSIVEKNGMSEEITSLYEELRALKCVLQFVRSGRVAITTSCFERVNEFLDGQEAKYK
QSKKEQE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory