SitesBLAST

E67 (= E70) binding Mg(2+)
D84 (= D86) binding Mg(2+)
L86 (= L88) binding Mg(2+)
D87 (= D89) mutation to N: Loss of IMPase activity, still complements dnaB121 helicase mutation.
R139 (≠ E141) mutation to C: In suhB10; decreases polypeptide chain elongation rate, grows at 30 but not 25 degrees Celsius; when associated with A-250.
G173 (≠ D175) mutation to V: No growth at 30 degrees Celsius, IMPase activity not inhibited by RNA polymerase (RNAP).
R183 (= R185) mutation to A: Some growth at 30 degrees Celsius, greater IMPase activity, partially inhibited by RNAP.
R184 (≠ M186) mutation to A: Grows at 30 degrees Celsius, makes more dimer, partially inhibited by RNAP, crystallizes.; mutation to I: No growth at 30 degrees Celsius, IMPase activity not inhibited by RNAP.

Sites not aligning to the query:

250 V→A: In suhB10; decreases polypeptide chain elongation rate, grows at 30 but not 25 degrees Celsius; when associated with C-139.
251:254 KAML→AAMA: 3-fold decreased affinity for NusA, less efficient in delaying or suppressing Rho-dependent transcription termination.

2qflA Structure of suhb: inositol monophosphatase and extragenic suppressor from e. Coli (see paper)
34% identity, 89% coverage: 12:248/267 of query aligns to 9:246/262 of 2qflA

query
sites
2qflA

C

V

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A

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A

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A

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N

active site: D44 (= D47), E67 (= E70), D84 (= D86), L86 (= L88), D87 (= D89), T89 (= T91), D212 (= D214)
binding ethyl acetate: D87 (= D89), G186 (= G188), S187 (= S189), A188 (= A190)

6ib8B Structure of a complex of suhb and nusa ar2 domain (see paper)
34% identity, 89% coverage: 12:248/267 of query aligns to 13:250/270 of 6ib8B

query
sites
6ib8B

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N

binding magnesium ion: D88 (= D86), L90 (= L88)

6tqoT Rrn anti-termination complex (see paper)
35% identity, 89% coverage: 12:248/267 of query aligns to 9:238/255 of 6tqoT

query
sites
6tqoT

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N

N

binding magnesium ion: E59 (= E70), D76 (= D86), L78 (= L88)
binding : Q126 (≠ F136), R133 (≠ H143)

Q9M8S8 Inositol-phosphate phosphatase; L-galactose 1-phosphate phosphatase; Myo-inositol monophosphatase; EC 3.1.3.25; EC 3.1.3.93 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 88% coverage: 26:259/267 of query aligns to 26:266/271 of Q9M8S8

query
sites
Q9M8S8

R

R

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P

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D

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G

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L

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D

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L

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L

A

L

L

P92 (= P87) mutation to L: In vtc4-1; low levels of ascorbate.

4as5A Structure of mouse inositol monophosphatase 1 (see paper)
32% identity, 94% coverage: 14:265/267 of query aligns to 13:269/274 of 4as5A

query
sites
4as5A

I

L

A

A

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L

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G

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A

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C

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L

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D

A

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A

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C

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W

D
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D

Y

M

S

A

A

G

A

A

A

G

L

I

I

I

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E

A

A

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G

R

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D

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G

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D

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F

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I

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P

L

L

active site: E68 (= E70), D88 (= D86), I90 (≠ L88), D91 (= D89), T93 (= T91), D218 (= D214)
binding magnesium ion: E68 (= E70), D88 (= D86), D88 (= D86), I90 (≠ L88), D91 (= D89), D91 (= D89), D218 (= D214)
binding phosphate ion: E68 (= E70), D88 (= D86), D91 (= D89), G92 (= G90)

3lv0A Crystal structure of extragenic suppressor protein suhb from bartonella henselae, native
33% identity, 91% coverage: 11:252/267 of query aligns to 7:247/258 of 3lv0A

query
sites
3lv0A

V

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active site: E66 (= E70), D83 (= D86), L85 (= L88), D86 (= D89), T88 (= T91), D209 (= D214)
binding magnesium ion: E66 (= E70), D83 (= D86), L85 (= L88)

O55023 Inositol monophosphatase 1; IMP 1; IMPase 1; D-galactose 1-phosphate phosphatase; Inositol-1(or 4)-monophosphatase 1; Lithium-sensitive myo-inositol monophosphatase A1; EC 3.1.3.25; EC 3.1.3.94 from Mus musculus (Mouse) (see paper)
31% identity, 94% coverage: 14:265/267 of query aligns to 15:271/277 of O55023

query
sites
O55023

I

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L

E70 (= E70) binding Mg(2+)
D90 (= D86) binding Mg(2+); binding Mg(2+)
I92 (≠ L88) binding Mg(2+)
D93 (= D89) binding Mg(2+)
D220 (= D214) binding Mg(2+)

3luzA Crystal structure of extragenic suppressor protein suhb from bartonella henselae, via combined iodide sad molecular replacement (see paper)
34% identity, 82% coverage: 34:252/267 of query aligns to 18:229/238 of 3luzA

query
sites
3luzA

V

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x
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x
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active site: E54 (= E70), D71 (= D86), L73 (= L88), D74 (= D89), T76 (= T91), D191 (= D214)
binding iodide ion: F49 (≠ A65), L197 (= L220), R200 (≠ L223)
binding magnesium ion: E54 (= E70), D71 (= D86), L73 (= L88)

Sites not aligning to the query:

binding iodide ion: 3

2hhmA Structure of inositol monophosphatase, the putative target of lithium therapy (see paper)
31% identity, 95% coverage: 14:266/267 of query aligns to 11:268/272 of 2hhmA

query
sites
2hhmA

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active site: D43 (= D47), E66 (= E70), D86 (= D86), I88 (≠ L88), D89 (= D89), T91 (= T91), D216 (= D214)
binding sulfate ion: E66 (= E70), D86 (= D86), D89 (= D89), G90 (= G90), T91 (= T91)

1imbA Structural analysis of inositol monophosphatase complexes with substrates (see paper)
31% identity, 95% coverage: 14:266/267 of query aligns to 11:268/272 of 1imbA

query
sites
1imbA

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x
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A

A

A

A

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L

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E

M

G

S

H

R

I

V

I

I

A

A

T

A

N

N

G

N

Y

R

L

I

H

L

P

A

L

E

F

R

L

I

K

A

L

K

L

E

A

I

E

Q

V

V

P

I

P

P

L

L

D

Q

active site: D43 (= D47), E66 (= E70), D86 (= D86), I88 (≠ L88), D89 (= D89), T91 (= T91), D216 (= D214)
binding l-myo-inositol-1-phosphate: D89 (= D89), G90 (= G90), S161 (≠ Y163), T191 (≠ S189), A192 (= A190), E209 (= E207), D216 (= D214)

1awbA Human myo-inositol monophosphatase in complex with d-inositol-1- phosphate and calcium
31% identity, 95% coverage: 14:266/267 of query aligns to 11:268/272 of 1awbA

query
sites
1awbA

I

L

A

A

T

R

E

Q

A

A

G

G

H

E

F

V

L

V

K

C

E

E

E

A

R

I

K

K

N

N

F

-

R

E

R

M

E

N

R

V

V

M

M

L

E

K

K

S

H

S

A

P

H

V

D

D

Y

L

V

V

S

T

Y

A

V

T

D
|
D

K

Q

E

K

S

V

E

E

V

K

R

M

L

L

V

I

K

S

A

S

L

I

S

K

A

E

L

K

L

Y

P

P

E

S

A

H

G

S

F

F

I

I

T

G

E
|
E

E

E

G

S

S

V

A

A

T

A

Y

G

Q

E

-

K

-

S

-

I

-

L

-

T

D

D

E

N

P

P

Y

T

C

-

W

W

V

I

I

I

D
|
D

P

P

L
x
I

D
|
D

G
|
G

T
|
T

T

T

N

N

Y

F

I

V

H

H

D

R

E

F

A

P

P

F

Y

V

C

A

V

V

C

S

I

I

A

G

L

F

R

A

S

V

R

N

T

K

E

K

L

I

L

E

L

F

G

G

V

V

Y

Y

E

S

V

C

C

V

R

E

N

G

E

K

C

M

F

Y

Y

T

A

A

W

R

K

K

G

G

G

K

K

G

A

A

F

F

M

C

N

N

G

G

E

Q

E

K

I

L

H

Q

V

V

S

S

D

Q

V

Q

R

E

D

D

I

I

K

T

N

K

A

S

F

L

V

L

F

V

T

T

E
|
E

L

L

P

-

Y

-

N

G

Y

S

D

S

Q

R

Y

T

K

P

P

E

T

T

A

V

L

R

H

M

L

V

I

L

D

S

N

N

L

M

Y

E

G

K

-

L

-

F

-

C

-

I

A

P

V

V

G

H

G

G

I

I

R

R

M

S

N

V

G
|
G

S
x
T

A
|
A

A

A

A

V

A

N

I

M

C

C

Y

L

I

V

A

A

N

T

G

G

R

G

F

A

D

D

A

A

W

Y

A

Y

E
|
E

A

M

F

G

I

I

G

H

K

C

W

W

D
|
D

Y

V

S

A

A

G

A

A

A

G

L

I

I

I

V

V

L

T

E

E

A

A

G

G

R

V

V

L

T

M

N

D

F

V

Y

T

G

G

D

G

D

P

H

F

F

D

I

L

E

M

G

S

H

R

I

V

I

I

A

A

T

A

N

N

G

N

Y

R

L

I

H

L

P

A

L

E

F

R

L

I

K

A

L

K

L

E

A

I

E

Q

V

V

P

I

P

P

L

L

D

Q

active site: D43 (= D47), E66 (= E70), D86 (= D86), I88 (≠ L88), D89 (= D89), T91 (= T91), D216 (= D214)
binding calcium ion: E66 (= E70), D86 (= D86), D86 (= D86), I88 (≠ L88), D89 (= D89), D216 (= D214)
binding d-myo-inositol-1-phosphate: D86 (= D86), D89 (= D89), G90 (= G90), E158 (= E158), G190 (= G188), T191 (≠ S189), A192 (= A190), E209 (= E207)

P29218 Inositol monophosphatase 1; IMP 1; IMPase 1; D-galactose 1-phosphate phosphatase; Inositol-1(or 4)-monophosphatase 1; Lithium-sensitive myo-inositol monophosphatase A1; EC 3.1.3.25; EC 3.1.3.94 from Homo sapiens (Human) (see 5 papers)
31% identity, 94% coverage: 14:265/267 of query aligns to 15:271/277 of P29218

query
sites
P29218

I

L

A

A

T

R

E

Q

A

A

G

G

H

E

F

V

L

V

K

C

E

E

E

A

R

I

K

K

N

N

F

-

R

E

R

M

E

N

R

V

V

M

M

L

E
x
K

K

S

H

S

A

P

H

V

D

D

Y

L

V

V

S

T

Y

A

V

T

D

D

K

Q

E

K

S

V

E

E

V

K

R

M

L

L

V

I

K

S

A

S

L

I

S

K

A

E

L

K

L

Y

P

P

E

S

A

H

G

S

F

F

I

I

T

G

E
|
E

E

E

G

S

S

V

A

A

T

A

Y

G

Q

E

-

K

-

S

-

I

-

L

-

T

D

D

E

N

P

P

Y

T

C

-

W

W

V

I

I

I

D
|
D

P

P

L
x
I

D
|
D

G
|
G

T
|
T

T

T

N

N

Y

F

I

V

H

H

D

R

E

F

A

P

P

F

Y

V

C

A

V

V

C

S

I

I

A

G

L

F

R

A

S

V

R

N

T

K

E

K

L

I

L

E

L

F

G

G

V

V

Y

Y

E

S

V

C

C

V

R

E

N

G

E

K

C

M

F

Y

Y

T

A

A

W

R

K

K

G

G

G

K

K

G

A

A

F

F

M

C

N

N

G

G

E

Q

E

K

I

L

H

Q

V

V

S

S

D

Q

V

Q

R

E

D

D

I

I

K

T

N

K

A

S

F

L

V

L

F

V

T

T

E

E

L

L

P

-

Y

-

N

G

Y
x
S

D

S

Q

R

Y

T

K

P

P

E

T

T

A

V

L

R

H

M

L

V

I

L

D

S

N

N

L

M

Y

E

G

K

-

L

-

F

-

C

-

I

A

P

V

V

G

H

G

G

I

I

R

R

M

S

N

V

G
|
G

S
x
T

A
|
A

A

A

A

V

A

N

I

M

C

C

Y

L

I

V

A

A

N

T

G

G

R

G

F

A

D

D

A

A

W

Y

A

Y

E
|
E

A

M

F

G

I

I

G

H

K

C

W

W

D
|
D

Y

V

S

A

A

G

A

A

A

G

L

I

I

I

V

V

L

T

E

E

A

A

G

G

R

V

V

L

T

M

N

D

F

V

Y

T

G

G

D

G

D

P

H

F

F

D

I

L

E

M

G

S

H

R

I

V

I

I

A

A

T

A

N

N

G

N

Y

R

L

I

H

L

P

A

L

E

F

R

L

I

K

A

L

K

L

E

A

I

E

Q

V

V

P

I

P

P

L

L

K36 (≠ E36) mutation to Q: 50-fold reduction in activity.
E70 (= E70) binding Mg(2+); binding substrate
D90 (= D86) binding Mg(2+); binding Mg(2+)
I92 (≠ L88) binding Mg(2+)
IDGT 92:95 (≠ LDGT 88:91) binding substrate
D93 (= D89) binding Mg(2+); mutation to N: Loss of activity.
S165 (≠ Y163) mutation S->A,I: Reduced enzyme activity with myo-inositol 1-phosphate.
GTA 194:196 (≠ GSA 188:190) binding substrate
E213 (= E207) binding substrate; mutation to Q: Strongly reduced affinity for myo-inositol 1-phosphate and strongly reduced enzyme activity with myo-inositol 1-phosphate.
D220 (= D214) binding Mg(2+); binding substrate

6zk0AAA human impase with ebselen (see paper)
31% identity, 95% coverage: 14:266/267 of query aligns to 12:269/274 of 6zk0AAA

query
sites
6zk0AAA

I

L

A

A

T

R

E

Q

A

A

G

G

H

E

F

V

L

V

K

C

E

E

E

A

R

I

K

K

N

N

F

-

R

E

R

M

E

N

R

V

V

M

M

L

E

K

K

S

H

S

A

P

H

V

D

D

Y

L

V

V

S

T

Y

A

V

T

D

D

K

Q

E

K

S

V

E

E

V

K

R

M

L

L

V

I

K

S

A

S

L

I

S

K

A

E

L

K

L

Y

P

P

E

S

A

H

G

S

F

F

I

I

T

G

E
|
E

E

E

G

S

S

V

A

A

T

A

Y

G

Q

E

-

K

-

S

-

I

-

L

-

T

D

D

E

N

P

P

Y

T

C

-

W

W

V

I

I

I

D
|
D

P

P

L
x
I

D
|
D

G

G

T

T

N

N

Y

F

I

V

H

H

D

R

E

F

A

P

P

F

Y

V

C

A

V

V

C

S

I

I

A

G

L

F

R

A

S

V

R

N

T

K

E

K

L

I

L

E

L

F

G

G

V

V

Y

Y

E

S

V

C

C

V

R

E

N

G

E
x
K

C

M

F

Y

Y

T

A

A

W

R

K

K

G

G

G

K

K

G

A

A

F

F

M
x
C

N
|
N

G

G

E
x
Q

E

K

I

L

H

Q

V

V

S

S

D

Q

V

Q

R

E

D

D

I

I

K

T

N

K

A

S

F

L

V

L

F

V

T

T

E

E

L

L

P

-

Y

-

N

G

Y

S

D

S

Q

R

Y

T

K

P

P

E

T

T

A

V

L

R

H

M

L

V

I

L

D

S

N

N

L

M

Y

E

G

K

-

L

-

F

-

C

-

I

A

P

V

V

G

H

G

G

I

I

R

R

M

S

N

V

G

G

S

T

A

A

A

V

A

N

I

M

C

C

Y

L

I

V

A

A

N

T

G

G

R

G

F

A

D

D

A

A

W

Y

A

Y

E

E

A

M

F

G

I

I

G

H

K

C

W

W

D

D

Y

V

S

A

A

G

A

A

A

G

L

I

I

I

V

V

L

T

E

E

A

A

G

G

R

V

V

L

T

M

N

D

F

V

Y

T

G

G

D

G

D

P

H

F

F

D

I

L

E

M

G

S

H

R

I

V

I

I

A

A

T

A

N

N

G

N

Y

R

L

I

H

L

P

A

L

E

F

R

L

I

K

A

L

K

L

E

A

I

E

Q

V

V

P

I

P

P

L

L

D

Q

binding N-phenyl-2-selanylbenzamide: K126 (≠ E125), C138 (≠ M137), N139 (= N138), Q141 (≠ E140)
binding manganese (ii) ion: E67 (= E70), D87 (= D86), I89 (≠ L88), D90 (= D89)

4as4A Structure of human inositol monophosphatase 1 (see paper)
31% identity, 95% coverage: 14:266/267 of query aligns to 13:270/274 of 4as4A

query
sites
4as4A

I

L

A

A

T

R

E

Q

A

A

G

G

H

E

F

V

L

V

K

C

E

E

E

A

R

I

K

K

N

N

F

-

R

E

R

M

E

N

R

V

V

M

M

L

E

K

K

S

H

S

A

P

H

V

D

D

Y

L

V

V

S

T

Y

A

V

T

D
|
D

K

Q

E

K

S

V

E

E

V

K

R

M

L

L

V

I

K

S

A

S

L

I

S

K

A

E

L

K

L

Y

P

P

E

S

A

H

G

S

F

F

I

I

T

G

E
|
E

E

E

G

S

S

V

A

A

T

A

Y

G

Q

E

-

K

-

S

-

I

-

L

-

T

D

D

E

N

P

P

Y

T

C

-

W

W

V

I

I

I

D
|
D

P

P

L
x
I

D
|
D

G
|
G

T
|
T

T

T

N

N

Y

F

I

V

H

H

D

R

E

F

A

P

P

F

Y

V

C

A

V

V

C

S

I

I

A

G

L

F

R

A

S

V

R

N

T

K

E

K

L

I

L

E

L

F

G

G

V

V

Y

Y

E

S

V

C

C

V

R

E

N

G

E

K

C

M

F

Y

Y

T

A

A

W

R

K

K

G

G

G

K

K

G

A

A

F

F

M

C

N

N

G

G

E

Q

E

K

I

L

H

Q

V

V

S

S

D

Q

V

Q

R

E

D

D

I

I

K

T

N

K

A

S

F

L

V

L

F

V

T

T

E

E

L

L

P

-

Y

-

N

G

Y

S

D

S

Q

R

Y

T

K

P

P

E

T

T

A

V

L

R

H

M

L

V

I

L

D

S

N

N

L

M

Y

E

G

K

-

L

-

F

-

C

-

I

A

P

V

V

G

H

G

G

I

I

R

R

M

S

N

V

G

G

S

T

A

A

A

V

A

N

I

M

C

C

Y

L

I

V

A

A

N

T

G

G

R

G

F

A

D

D

A

A

W

Y

A

Y

E

E

A

M

F

G

I

I

G

H

K

C

W

W

D
|
D

Y

V

S

A

A

G

A

A

A

G

L

I

I

I

V

V

L

T

E

E

A

A

G

G

R

V

V

L

T

M

N

D

F

V

Y

T

G

G

D

G

D

P

H

F

F

D

I

L

E

M

G

S

H

R

I

V

I

I

A

A

T

A

N

N

G

N

Y

R

L

I

H

L

P

A

L

E

F

R

L

I

K

A

L

K

L

E

A

I

E

Q

V

V

P

I

P

P

L

L

D

Q

active site: D45 (= D47), E68 (= E70), D88 (= D86), I90 (≠ L88), D91 (= D89), T93 (= T91), D218 (= D214)
binding magnesium ion: E68 (= E70), D88 (= D86), D88 (= D86), I90 (≠ L88), D91 (= D89), D218 (= D214)
binding phosphate ion: E68 (= E70), D88 (= D86), I90 (≠ L88), D91 (= D89), G92 (= G90), T93 (= T91)

6giuA Human impase with l-690330 (see paper)
31% identity, 94% coverage: 14:265/267 of query aligns to 13:269/275 of 6giuA

query
sites
6giuA

I

L

A

A

T

R

E

Q

A

A

G

G

H

E

F

V

L

V

K

C

E

E

E

A

R

I

K

K

N

N

F

-

R

E

R

M

E

N

R

V

V

M

M

L

E

K

K

S

H

S

A

P

H

V

D

D

Y

L

V

V

S

T

Y

A

V

T

D

D

K

Q

E

K

S

V

E

E

V

K

R

M

L

L

V

I

K

S

A

S

L

I

S

K

A

E

L

K

L

Y

P

P

E

S

A

H

G

S

F

F

I

I

T

G

E
|
E

E

E

G

S

S

V

A

A

T

A

Y

G

Q

E

-

K

-

S

-

I

-

L

-

T

D

D

E

N

P

P

Y

T

C

-

W

W

V

I

I

I

D
|
D

P

P

L
x
I

D
|
D

G
|
G

T
|
T

T

T

N

N

Y

F

I

V

H

H

D

R

E

F

A

P

P

F

Y

V

C

A

V

V

C

S

I

I

A

G

L

F

R

A

S

V

R

N

T

K

E

K

L

I

L

E

L

F

G

G

V

V

Y

Y

E

S

V

C

C

V

R

E

N

G

E

K

C

M

F

Y

Y

T

A

A

W

R

K

K

G

G

G

K

K

G

A

A

F

F

M

C

N

N

G

G

E

Q

E

K

I

L

H

Q

V

V

S

S

D

Q

V

Q

R

E

D

D

I

I

K

T

N

K

A

S

F

L

V

L

F

V

T

T

E
|
E

L

L

P

-

Y

-

N

G

Y

S

D

S

Q

R

Y

T

K

P

P

E

T

T

A

V

L

R

H

M

L

V

I

L

D

S

N

N

L

M

Y

E

G

K

-

L

-

F

-

C

-

I

A

P

V

V

G

H

G

G

I

I

R

R

M

S

N

V

G
|
G

S

T

A
|
A

A

A

A

V

A

N

I

M

C

C

Y

L

I

V

A

A

N

T

G

G

R

G

F

A

D

D

A

A

W

Y

A

Y

E
|
E

A

M

F

G

I

I

G

H

K

C

W
|
W

D
|
D

Y

V

S

A

A

G

A

A

A

G

L

I

I

I

V

V

L

T

E

E

A

A

G

G

R

V

V

L

T

M

N

D

F

V

Y

T

G

G

D

G

D

P

H

F

F

D

I

L

E

M

G

S

H

R

I

V

I

I

A

A

T

A

N

N

G

N

Y

R

L

I

H

L

P

A

L

E

F

R

L

I

K

A

L

K

L

E

A

I

E

Q

V

V

P

I

P

P

L

L

active site: E68 (= E70), D88 (= D86), I90 (≠ L88), D91 (= D89), T93 (= T91), D218 (= D214)
binding [1-(4-oxidanylphenoxy)-1-phosphono-ethyl]phosphonic acid: E68 (= E70), D88 (= D86), D91 (= D89), G92 (= G90), T93 (= T91), E160 (= E158), G192 (= G188), A194 (= A190), E211 (= E207), W217 (= W213), D218 (= D214)
binding manganese (ii) ion: E68 (= E70), D88 (= D86), D88 (= D86), I90 (≠ L88), D91 (= D89), D91 (= D89), D218 (= D214)

1imdA Structural studies of metal binding by inositol monophosphatase: evidence for two-metal ion catalysis (see paper)
32% identity, 89% coverage: 14:251/267 of query aligns to 11:253/266 of 1imdA

query
sites
1imdA

I

L

A

A

T

R

E

Q

A

A

G

G

H

E

F

V

L

V

K

C

E

E

E

A

R

I

K

K

N

N

F

-

R

E

R

M

E

N

R

V

V

M

M

L

E

K

K

S

H

S

A

P

H

V

D

D

Y

L

V

V

S

T

Y

A

V

T

D
|
D

K

Q

E

K

S

V

E

E

V

K

R

M

L

L

V

I

K

S

A

S

L

I

S

K

A

E

L

K

L

Y

P

P

E

S

A

H

G

S

F

F

I

I

T

G

E
|
E

E

E

G

S

S

V

A

A

T

A

Y

G

Q

E

-

K

-

S

-

I

-

L

-

T

D

D

E

N

P

P

Y

T

C

-

W

W

V

I

I

I

D
|
D

P

P

L
x
I

D
|
D

G
|
G

T
|
T

T

T

N

N

Y

F

I

V

H

H

D

R

E

F

A

P

P

F

Y

V

C

A

V

V

C

S

I

I

A

G

L

F

R

A

S

V

R

N

T

K

E

K

L

I

L

E

L

F