SitesBLAST
Comparing 352330 FitnessBrowser__Btheta:352330 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
45% identity, 92% coverage: 24:319/321 of query aligns to 3:298/306 of 4xckA
- active site: A249 (≠ G270), A250 (= A271), G251 (= G272), D252 (= D273)
- binding adenosine-5'-diphosphate: T220 (= T241), G222 (= G243), S223 (≠ K244), V242 (= V263), T247 (= T268), A250 (= A271), F254 (= F275), H276 (≠ N297), A279 (= A300), V283 (= V304)
- binding alpha-D-ribofuranose: N11 (= N32), D13 (= D34), G39 (= G60), K40 (= K61), N43 (= N64), A95 (= A116), I107 (= I128), I109 (≠ V130), E140 (= E161), T248 (= T269), D252 (= D273)
8cqxA Ribokinase from t.Sp mutant a92g
42% identity, 92% coverage: 25:319/321 of query aligns to 2:294/300 of 8cqxA
- binding adenosine-5'-diphosphate: N179 (= N205), T217 (= T241), G219 (= G243), A220 (≠ K244), G222 (= G246), F250 (= F275), N272 (= N297), G275 (≠ A300), A276 (= A301), T279 (≠ V304)
- binding magnesium ion: D242 (= D267), T244 (= T269), A278 (= A303), S287 (≠ A312)
P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 3 papers)
42% identity, 92% coverage: 25:319/321 of query aligns to 7:301/309 of P0A9J6
1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121 (see paper)
42% identity, 92% coverage: 25:319/321 of query aligns to 4:298/305 of 1rk2A
- active site: A249 (≠ G270), A250 (= A271), G251 (= G272), D252 (= D273)
- binding adenosine-5'-diphosphate: T220 (= T241), G222 (= G243), S223 (≠ K244), A250 (= A271), G251 (= G272), H276 (≠ N297), A279 (= A300)
- binding tetrafluoroaluminate ion: G213 (= G234), R215 (≠ E236)
- binding magnesium ion: D246 (= D267), A282 (= A303), R285 (= R306), S291 (≠ A312)
- binding alpha-D-ribofuranose: N11 (= N32), D13 (= D34), G38 (= G59), G39 (= G60), K40 (= K61), N43 (= N64), E140 (= E161), D252 (= D273)
1gqtB Activation of ribokinase by monovalent cations (see paper)
42% identity, 92% coverage: 25:319/321 of query aligns to 6:300/307 of 1gqtB
- active site: A251 (≠ G270), A252 (= A271), G253 (= G272), D254 (= D273)
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N205), T222 (= T241), G224 (= G243), S225 (≠ K244), A252 (= A271), G253 (= G272), H278 (≠ N297), A281 (= A300)
- binding cesium ion: D248 (= D267), I250 (≠ T269), A284 (= A303), R287 (= R306), S293 (≠ A312)
- binding alpha-D-ribofuranose: N13 (= N32), D15 (= D34), G41 (= G60), N45 (= N64), E142 (= E161), D254 (= D273)
2fv7A Crystal structure of human ribokinase
39% identity, 93% coverage: 25:321/321 of query aligns to 4:305/308 of 2fv7A
- active site: G252 (= G270), A253 (= A271), G254 (= G272), D255 (= D273)
- binding adenosine-5'-diphosphate: N185 (= N205), T221 (= T241), G223 (= G243), G226 (= G246), T242 (≠ A260), V245 (= V263), A253 (= A271), G254 (= G272), N281 (= N297), A284 (= A300), A285 (= A301), V288 (= V304)
6wjzA Crystal structure of human ribokinase in complex with ampcp
39% identity, 93% coverage: 25:321/321 of query aligns to 5:306/315 of 6wjzA
- binding phosphomethylphosphonic acid adenosyl ester: N186 (= N205), T222 (= T241), G224 (= G243), A225 (≠ K244), G227 (= G246), T243 (≠ A260), V246 (= V263), A254 (= A271), G255 (= G272), N282 (= N297), A285 (= A300), A286 (= A301), V289 (= V304)
5c3yA Structure of human ribokinase crystallized with amppnp
39% identity, 93% coverage: 25:321/321 of query aligns to 4:305/306 of 5c3yA
- active site: G252 (= G270), A253 (= A271), G254 (= G272), D255 (= D273)
- binding amp phosphoramidate: T221 (= T241), G223 (= G243), V245 (= V263), T250 (= T268), G254 (= G272), N281 (= N297), A284 (= A300), A285 (= A301)
6wk0B Crystal structure of human ribokinase in complex with amppcp and ribose
39% identity, 93% coverage: 25:321/321 of query aligns to 5:306/311 of 6wk0B
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N205), T222 (= T241), G224 (= G243), A225 (≠ K244), G227 (= G246), T243 (≠ A260), V246 (= V263), A254 (= A271), G255 (= G272), N282 (= N297), A285 (= A300), A286 (= A301), V289 (= V304)
- binding alpha-D-ribofuranose: D14 (= D34), G40 (= G60), K41 (= K61), N44 (= N64), A96 (= A116), E141 (= E161), D256 (= D273)
5c41A Crystal structure of human ribokinase in complex with amppcp in p21 spacegroup and with 4 protomers
39% identity, 93% coverage: 25:321/321 of query aligns to 5:306/317 of 5c41A
- active site: G253 (= G270), A254 (= A271), G255 (= G272), D256 (= D273)
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N205), T222 (= T241), G224 (= G243), A225 (≠ K244), G227 (= G246), V246 (= V263), G255 (= G272), N282 (= N297), A285 (= A300), A286 (= A301)
5byfA Crystal structure of human ribokinase in complex with amp
39% identity, 91% coverage: 29:321/321 of query aligns to 10:307/313 of 5byfA
Q9H477 Ribokinase; RK; EC 2.7.1.15 from Homo sapiens (Human)
39% identity, 91% coverage: 29:321/321 of query aligns to 22:319/322 of Q9H477
6znxC Ribokinase from thermus species
37% identity, 92% coverage: 25:319/321 of query aligns to 2:259/265 of 6znxC
6a8cA Ribokinase from leishmania donovani with adp (see paper)
36% identity, 93% coverage: 23:319/321 of query aligns to 14:322/327 of 6a8cA
- binding adenosine-5'-diphosphate: G245 (= G243), A246 (≠ K244), T271 (= T268), A274 (= A271), G275 (= G272), N300 (= N297), A303 (= A300)
- binding glycerol: D25 (= D34), S42 (≠ G51), S44 (≠ T53), G50 (= G59), G51 (= G60), N55 (= N64)
6a8bA Ribokinase from leishmania donovani with amppcp (see paper)
36% identity, 93% coverage: 23:319/321 of query aligns to 14:322/327 of 6a8bA
- binding phosphomethylphosphonic acid adenylate ester: G245 (= G243), A246 (≠ K244), T271 (= T268), A274 (= A271), G275 (= G272), N300 (= N297), A303 (= A300), V307 (= V304)
- binding glycerol: D25 (= D34), G50 (= G59), G51 (= G60), N55 (= N64), N157 (≠ L160), I159 (= I162), E190 (vs. gap)
6a8aA Ribokinase from leishmania donovani with atp (see paper)
36% identity, 93% coverage: 23:319/321 of query aligns to 14:322/327 of 6a8aA
- binding adenosine-5'-triphosphate: N207 (= N205), T243 (= T241), G245 (= G243), A246 (≠ K244), G248 (= G246), T271 (= T268), G273 (= G270), A274 (= A271), G275 (= G272), N300 (= N297), A303 (= A300), V307 (= V304)
- binding glycerol: D25 (= D34), G50 (= G59), G51 (= G60), N55 (= N64)
A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 96% coverage: 13:320/321 of query aligns to 59:372/379 of A1A6H3
Sites not aligning to the query:
- 1:74 mutation Missing: Loss of ribokinase activity.
6ilsB Structure of arabidopsis thaliana ribokinase complexed with ribose and atp (see paper)
33% identity, 93% coverage: 23:320/321 of query aligns to 3:306/313 of 6ilsB
- binding adenosine-5'-triphosphate: N189 (= N205), K225 (≠ T241), G227 (= G243), I246 (= I258), A248 (= A260), A257 (= A271), G258 (= G272), F261 (= F275), A286 (= A300), S287 (≠ A301)
- binding alpha-D-ribofuranose: N12 (= N32), D14 (= D34), G40 (= G60), K41 (= K61), N44 (= N64), E144 (= E161), D259 (= D273)
3go6B Crystal structure of m. Tuberculosis ribokinase (rv2436) in complex with ribose and amp-pnp
35% identity, 80% coverage: 23:280/321 of query aligns to 2:237/287 of 3go6B
- active site: G227 (= G270), A228 (= A271), G229 (= G272), D230 (= D273)
- binding adenosine-5'-diphosphate: G200 (= G243), V201 (≠ K244), G203 (= G246), V220 (= V263), T225 (= T268), A228 (= A271), G229 (= G272)
- binding magnesium ion: D224 (= D267), A226 (≠ T269)
Sites not aligning to the query:
3go6A Crystal structure of m. Tuberculosis ribokinase (rv2436) in complex with ribose and amp-pnp
35% identity, 80% coverage: 23:280/321 of query aligns to 2:237/287 of 3go6A
- active site: G227 (= G270), A228 (= A271), G229 (= G272), D230 (= D273)
- binding magnesium ion: D224 (= D267)
- binding alpha-D-ribofuranose: N11 (= N32), D13 (= D34), G39 (= G60), K40 (= K61), N43 (= N64), A94 (= A116), I96 (= I118), V106 (≠ I128), E134 (= E161), D230 (= D273)
Sites not aligning to the query:
Query Sequence
>352330 FitnessBrowser__Btheta:352330
MIIAFICIVLIFNTMETISIHRPKIVVIGSCNTDMVVKANRLPVPGETILGGTFYMNPGG
KGANQAIAAARLGAEVTFISKIGYDLFGLQALEIYRSEKINTEYIFTDQKSPSGVALISV
DSFGENSIIVAPGASRSLSIEDINKAEEKIKEADIILMQLEIPIDTVEYAATIACKYGKK
VILNPAPASSLSNSFLMNVHTILPNRIEAEMLSGIKVMNIESAHRAAQAIGEKGIENVVI
TLGKDGAYVKEKDEYTMIPAKEVETIDTTGAGDVFCGAFSVCLSEGHSLAKSVKFANAAA
AIAVTRIGAQSAIPYKREVVL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory