SitesBLAST

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
44% identity, 63% coverage: 18:228/336 of query aligns to 11:218/304 of Q96XT4

query
sites
Q96XT4

W

W

C
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C

P

P

G

G

C
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C

G

G

D

D

H

F

A

G

F

I

L

L

N

R

S

A

L

E

H

E

K

M

A

A

M

I

A

R

E

E

L

L

G

G

V

I

A

N

P

P

H

K

N

S

I

V

A

V

V

I

I

V

S

S

G

G

I
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I

G
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G

C
|
C

S
|
S

S

G

R

K

L

I

P

P

Y

H

Y

F

V

M

N

N

-

L

-

P

T

I

Y

S

G

G

F

V

H

H

T

T

I

L

H

H

G

G

R

R

A

S

A

I

A

A

V

F

A

A

T

T

G

G

A

I

K

K

V

L

A

S

N

N

P

P

D

S

L

L

T

E

I

V

W

I

Q

V

I

N

S

V

G

G

D
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D

G
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G

D
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D

G

G

L

L

A

G

I

I

G

G

G

M

N

G

H

H

F

F

I

V

H

H

A

L

V

G

R

R

N

N

I

I

D

D

L

I

N

A

M

V

I

L

L

V

L

H

N

N

N
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N

R

G

I
x
V

Y

Y

G
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G

L
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L

T

T

K

K

G

G

Q

Q

Y

A

S

S

P

P

T

T

S

L

E

H

R

R

G

G

F

E

V

K

S

T

K

K

S

S

S

L

P

P

Y

K

G

P

T

N

V

I

E

M

D

D

P

A

F

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H

N

P

P

A

L

E

A

L

V

A

A

F

L

G

A

A

A

R

G

G

Y

R

T

F

F

F

V

A

A

R

R

C

G

I

Y

A

A

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Y

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D

G

V

A

M

A

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S

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E

E

V

L

L

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K

A

K

A

A

A

I

N

L

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H

K

K

G

G

A

S

S

A

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L

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V

E

D

V

I

L

L

Q

Q

N

P

C
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C

V

P

I

T

F

Y

N

N

D

D

G

-

T

-

H

-

A

-

S

-

V

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A

N

T

T

K

K

E

E

G

W

R

Y

A

D

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K

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Y

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L

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D

H

N

C12 (= C19) binding [4Fe-4S] cluster
C15 (= C22) binding [4Fe-4S] cluster
IGCS 44:47 (= IGCS 51:54) binding thiamine diphosphate
C46 (= C53) binding [4Fe-4S] cluster
D90 (= D95) binding Mg(2+)
GD 91:92 (= GD 96:97) binding thiamine diphosphate
N118 (= N123) binding Mg(2+)
V120 (≠ I125) binding Mg(2+)
GL 122:123 (= GL 127:128) binding thiamine diphosphate
C197 (= C202) binding [4Fe-4S] cluster

5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
44% identity, 63% coverage: 18:228/336 of query aligns to 8:215/301 of 5b46B

query
sites
5b46B

W
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W

C
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C

P

P

G

G

C
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C

G

G

D

D

H

F

A

G

F

I

L

L

N

R

S

A

L

E

H

E

K

M

A

A

M

I

A

R

E

E

L

L

G

G

V

I

A

N

P

P

H

K

N

S

I

V

A

V

V

I

I

V

S

S

G

G

I
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I

G
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G

C
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C

S
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S

S

G

R

K

L

I

P

P

Y

H

Y

F

V

M

N

N

-

L

-

P

T

I

Y

S

G

G

F

V

H

H

T

T

I

L

H
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H

G

G

R

R

A

S

A

I

A

A

V

F

A

A

T

T

G

G

A

I

K

K

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A

S

N

N

P

P

D

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L

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I

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W

I

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G

D
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D

G
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G

D
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D

G

G

L

L

A

G

I

I

G

G

G

M

N

G

H

H

F

F

I

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H

H

A

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G

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R

N

N

I

I

D

D

L

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N

A

M

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I

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L

V

L

H

N

N

N
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N

R

G

I
x
V

Y
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Y

G
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G

L
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L

T
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T

K

K

G

G

Q

Q

Y

A

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S

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P

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T

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R

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G

F

E

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K

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S

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P

Y

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D

P

A

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N

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P

A

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A

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A

A

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A

A

A

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G

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F

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A

A

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C

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A

A

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D

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L

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K

A

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K

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G

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S

A

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E

D

V

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L

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Q

N

P

C
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C

V

P

I
x
T

F
x
Y

N

N

D

D

G

-

T

-

H

-

A

-

S

-

V

I

A

N

T

T

K

K

E

E

G

W

R

Y

A

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N

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Y

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H

N

binding magnesium ion: D87 (= D95), N115 (= N123), V117 (≠ I125)
binding iron/sulfur cluster: W8 (= W18), C9 (= C19), C12 (= C22), C43 (= C53), C194 (= C202), T196 (≠ I204), Y197 (≠ F205)
binding thiamine diphosphate: I41 (= I51), G42 (= G52), C43 (= C53), S44 (= S54), H62 (= H70), G86 (= G94), G88 (= G96), D89 (= D97), N115 (= N123), V117 (≠ I125), Y118 (= Y126), G119 (= G127), L120 (= L128), T121 (= T129)

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
46% identity, 63% coverage: 18:227/336 of query aligns to 11:217/305 of P72579

query
sites
P72579

W

W

C

C

P

P

G

G

C

C

G

G

D

N

H

F

A

G

F

I

L

L

N

N

S

A

L

E

H

Q

K

Q

A

A

M

I

A

V

E

E

L

L

G

G

V

V

A

D

P

T

H

K

N

N

I

V

A

V

V

V

I

V

S

S

G

G

I

I

G

G

C

C

S

S

S

G

R
x
K

L

I

P

P

Y

H

Y

F

V

F

N

R

T

T

-

P

-

I

Y

S

G

G

F

V

H

H

T

T

I

L

H

H

G

G

R

R

A

A

A

I

A

A

V

F

A

A

T

T

G

G

A

I

K

K

V

L

A

S

N

N

P

P

D

D

L

L

T

V

I

V

W

I

Q

V

I

N

S

G

G

G

D

D

G

G

D

D

G

L

L

L

A

G

I

I

G

G

G

A

N

G

H

H

F

F

I

V

H

A

A

A

V

G

R

R

N

N

I

V

D

D

L

M

N

V

M

V

I

I

L

L

L

H

N

D

N

N

R

G

I

V

Y

Y

G

G

L
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L

T

T

K

K

G

G

Q

Q

Y

A

S

S

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P

T

T

S

L

E

K

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R

G

G

F

E

V

K

S

P

K

K

S

S

S

L

P

P

Y

R

G

P

T

N

V

I

E

N

D

D

P

A

F

V

H

N

P

P

A

I

E

A

L

L

A

A

F

I

G

S

A

S

R

G

G

Y

R

T

F

F

F

V

A

A

R

R

C

G

I

Y

A

A

V

Y

D

D

G

V

A

K

A

H

S

L

V

K

E

E

V

L

L

I

K

K

A

S

A

A

A

I

N

K

H

H

K

K

G

G

A

L

S

A

V

L

V

I

E

D

V

V

L

L

Q

Q

N

P

C

C

V

P

I

T

F

Y

N

N

D

D

G

-

T

-

H

-

A

-

S

-

V

I

A

N

T

T

K

K

E

E

G

W

R

Y

A

D

K

K

N

R

A

I

I

Y

Y

K

L

L

E

D

K49 (≠ R56) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (= L128) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
46% identity, 63% coverage: 18:227/336 of query aligns to 11:217/305 of Q96Y68

query
sites
Q96Y68

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

N

H

F

A

G

F

I

L

L

N

N

S

A

L

E

H

Q

K

Q

A

A

M

I

A

V

E

E

L

L

G

G

V

V

A

D

P

T

H

K

N

N

I

V

A

V

V

V

I

V

S

S

G

G

I

I

G

G

C
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C

S

S

S

G

R
x
K

L

I

P

P

Y

H

Y

F

V

F

N

R

T

T

-

P

-

I

Y

S

G

G

F

V

H

H

T

T

I

L

H

H

G

G

R

R

A

A

A

I

A

A

V

F

A

A

T

T

G

G

A

I

K

K

V

L

A

S

N

N

P

P

D

D

L

L

T

V

I

V

W

I

Q

V

I

N

S

G

G

G

D
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D

G
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G

D
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D

G

L

L

L

A

G

I

I

G

G

G

A

N

G

H

H

F

F

I

V

H

A

A

A

V

G

R

R

N

N

I

V

D

D

L

M

N

V

M

V

I

I

L

L

L

H

N

D

N
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N

R

G

I
x
V

Y

Y

G
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G

L
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L

T

T

K
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K

G

G

Q

Q

Y

A

S

S

P

P

T

T

S

L

E

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R

R

G

G

F

E

V

K

S

P

K

K

S

S

S

L

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Y

R

G

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T

N

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N

D

D

P

A

F

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N

P

P

A

I

E

A

L

L

A

A

F

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G

S

A

S

R

G

G

Y

R

T

F

F

F

V

A

A

R

R

C

G

I

Y

A

A

V

Y

D

D

G

V

A
x
K

A

H

S

L

V

K

E

E

V

L

L

I

K

K

A

S

A

A

A

I

N

K

H

H

K

K

G

G

A

L

S

A

V

L

V

I

E

D

V

V

L

L

Q

Q

N

P

C
|
C

V

P

I

T

F

Y

N

N

D

D

G

-

T

-

H

-

A

-

S

-

V

I

A

N

T

T

K

K

E

E

G

W

R

Y

A

D

K

K

N

R

A

I

I

Y

Y

K

L

L

E

D

C12 (= C19) binding [4Fe-4S] cluster
C15 (= C22) binding [4Fe-4S] cluster
C46 (= C53) binding [4Fe-4S] cluster
K49 (≠ R56) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D95) binding Mg(2+)
GD 91:92 (= GD 96:97) binding thiamine diphosphate
N118 (= N123) binding Mg(2+)
V120 (≠ I125) binding Mg(2+)
GL 122:123 (= GL 127:128) binding thiamine diphosphate
K125 (= K130) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (≠ A178) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C202) binding [4Fe-4S] cluster

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
44% identity, 64% coverage: 18:233/336 of query aligns to 7:209/286 of 5b48B

query
sites
5b48B

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

N

H

F

A

G

F

I

L

L

N

N

S

A

L

E

H

Q

K

Q

A

A

M

I

A

V

E

E

L

L

G

G

V

V

A

D

P

T

H

K

N

N

I

V

A

V

V

V

I

V

S

S

G

G

I
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I

G
|
G

C
|
C

S
|
S

S

G

R

K

L

I

P

P

Y

H

Y

F

V

T

N

P

T

I

Y

S

G

G

F

V

H

H

T

T

I

L

H
|
H

G

G

R

R

A

A

A

I

A

A

V

F

A

A

T

T

G

G

A

I

K

K

V

L

A

S

N

N

P

P

D

D

L

L

T

V

I

V

W

I

Q

V

I

N

S

G

G

G

D
|
D

G
|
G

D
|
D

G

L

L

L

A

G

I

I

G

G

G

A

N

G

H

H

F

F

I

V

H

A

A

A

V

G

R

R

N

N

I

V

D

D

L

M

N

V

M

V

I

I

L

L

L

H

N

D

N
|
N

R

G

I
x
V

Y
|
Y

G
|
G

L
|
L

T

T

K

K

G

G

Q

Q

Y

A

S

S

P

P

T

T

S

L

E

K

R

R

G

G

F

-

V

-

S

-

K

-

S

-

P

-

Y

-

G

E

T

N

V

I

E

N

D

D

P

A

F

V

H

N

P

P

A

I

E

A

L

L

A

A

F

I

G

S

A

S

R

G

G

Y

R

T

F

F

F

V

A

A

R

R

C

G

I

Y

A

A

V

Y

D

D

G

V

A

K

A

H

S

L

V

K

E

E

V

L

L

I

K

K

A

S

A

A

A

I

N

K

H

H

K

K

G

G

A

L

S

A

V

L

V

I

E

D

V

V

L

L

Q

Q

N

P

C
|
C

V

P

I
x
T

F

Y

N

N

D

D

G

-

T

-

H

-

A

-

S

-

V

I

A

N

T

T

K

K

E

E

G

W

R

R

A

I

K

Y

N

K

A

L

I

D

Y

T

L

L

E

P

H

D

G

W

K

D

P

P

M

V

L

V

binding magnesium ion: D84 (= D95), N112 (= N123), V114 (≠ I125)
binding iron/sulfur cluster: C8 (= C19), C11 (= C22), C42 (= C53), C183 (= C202), T185 (≠ I204)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I51), G41 (= G52), C42 (= C53), S43 (= S54), H59 (= H70), G85 (= G96), D86 (= D97), N112 (= N123), V114 (≠ I125), Y115 (= Y126), G116 (= G127), L117 (= L128)

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
43% identity, 55% coverage: 18:202/336 of query aligns to 4:179/275 of 5b47B

query
sites
5b47B

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

D

H

F

A

G

F

I

L

L

N

R

S

A

L

E

H

E

K

M

A

A

M

I

A

R

E

E

L

L

G

G

V

I

A

N

P

P

H

K

N

S

I

V

A

V

V

I

I

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

S

G

R

K

L

I

P

P

Y

H

Y

F

V

M

N

N

-

L

-

P

T

I

Y

S

G

G

F

V

H

H

T

T

I

L

H
|
H

G

G

R

R

A

S

A

I

A

A

V

F

A

A

T

T

G

G

A

I

K

K

V

L

A

S

N

N

P

P

D

S

L

L

T

E

I

V

W

I

Q

V

I

N

S

V

G

G

D
|
D

G
|
G

D
|
D

G

G

L

L

A

G

I

I

G

G

G

M

N

G

H

H

F

F

I

V

H

H

A

L

V

G

R

R

N

N

I

I

D

D

L

I

N

A

M

V

I

L

L

V

L

H

N

N

N
|
N

R

G

I
x
V

Y
|
Y

G

G

L
|
L

T

-

K

-

G

-

Q

Q

Y

A

S

S

P

P

T

T

S

L

E

H

R

R

G

G

F

-

V

-

S

-

K

-

S

-

P

-

Y

-

G

E

T

N

V

I

E

M

D

D

P

A

F

V

H

N

P

P

A

L

E

A

L

V

A

A

F

L

G

A

A

A

R

G

G

Y

R

T

F

F

F

V

A

A

R

R

C

G

I

Y

A

A

V

Y

D

D

G

V

A

M

A

H

S

L

V

K

E

E

V

L

L

I

K

K

A

K

A

A

A

I

N

L

H

H

K

K

G

G

A

S

S

A

V

L

V

V

E

D

V

I

L

L

Q

Q

N

P

C
|
C

binding magnesium ion: D83 (= D95), N111 (= N123)
binding iron/sulfur cluster: C5 (= C19), C8 (= C22), C39 (= C53), C179 (= C202)
binding thiamine diphosphate: I37 (= I51), G38 (= G52), C39 (= C53), S40 (= S54), H58 (= H70), G84 (= G96), D85 (= D97), N111 (= N123), V113 (≠ I125), Y114 (= Y126), L116 (= L128)

6cioA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with lactyl-tpp bound (see paper)
27% identity, 47% coverage: 88:246/336 of query aligns to 959:1110/1164 of 6cioA

query
sites
6cioA

T

S

I

I

W

W

Q

I

I

I

S

G

G
|
G

D
|
D

G
|
G

D

W

G

A

L

Y

A

D

I

I

G

G

G

Y

N

G

H

G

F

L

I

D

H

H

A

V

V

L

R

A

R

S

N

G

I

A

D

N

L

V

N

N

M

V

I

L

L

V

L

L

N

D

N
x
T

R

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

Y

S

S

S

P

K

T

A

S

T

E

Q

R

T

G

G

F

A

V

V

S

A

K

R

S

F

S

A

P

A

Y

G

G

G

T

K

V

F

E

T

D

K

P

K

F

K

H

D

P

L

A

G

E

L

L

M

A

A

F

M

G

-

A

S

R

Y

G

G

R

Y

F

V

F

Y

A

V

R

A

C

S

I

V

A

A

V

M

D

-

G

G

A

A

A

S

S

H

V

S

E

Q

V

L

L

M

K

K

A

A

-

L

-

I

-

E

A

A

A

E

N

K

H

Y

K

D

G

G

A

P

S

S

V

L

V

I

E

I

V

A

L

Y

Q

A

N

P

C
|
C

V
x
I

I

-

F

-

N

N

D

H

G

G

T

I

H

N

A

M

S

T

V

Y

A

S

T

Q

K

R

E

E

G

-

R

-

A

A

K

K

N

K

A

A

I

V

Y

-

L

-

E

E

H

A

G

G

K

Y

P

W

M

P

L

L

F

Y

G

R

E

Y

N

N

K

P

E

Q

F

-

G

-

L

L

M

A

Q

Q

E

E

G

G

active site: N999 (≠ L128)
binding magnesium ion: D966 (= D95), T994 (≠ N123), V996 (≠ I125)
binding iron/sulfur cluster: C1074 (= C202), I1075 (≠ V203)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: G965 (= G94), D966 (= D95), G967 (= G96), V996 (≠ I125), Y997 (= Y126), S998 (≠ G127), N999 (≠ L128), T1000 (= T129)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 26, 27, 28, 61, 85, 111, 813, 834, 835, 836, 865

Q2RMD6 Pyruvate:ferredoxin oxidoreductase; PFOR; Pyruvate synthase; EC 1.2.7.1 from Moorella thermoacetica (strain ATCC 39073 / JCM 9320) (see paper)
27% identity, 47% coverage: 88:246/336 of query aligns to 960:1111/1171 of Q2RMD6

query
sites
Q2RMD6

T

S

I

I

W

W

Q

I

I

I

S

G

G

G

D
|
D

G
|
G

D
x
W

G

A

L

Y

A

D

I

I

G

G

G

Y

N

G

H

G

F

L

I

D

H

H

A

V

V

L

R

A

R

S

N

G

I

A

D

N

L

V

N

N

M

V

I

L

L

V

L

L

N

D

N
x
T

R
x
E

I
x
V

Y
|
Y

G
x
S

L
x
N

T

T

K

G

G

G

Q

Q

Y

S

S

S

P

K

T

A

S

T

E

Q

R

T

G

G

F

A

V

V

S

A

K

R

S

F

S

A

P

A

Y

G

G

G

T

K

V

F

E

T

D

K

P

K

F

K

H

D

P

L

A

G

E

L

L

M

A

A

F

M

G

-

A

S

R

Y

G

G

R

Y

F

V

F

Y

A

V

R

A

C

S

I

V

A

A

V

M

D

-

G

G

A

A

A

S

S

H

V

S

E

Q

V

L

L

M

K

K

A

A

-

L

-

I

-

E

A

A

A

E

N

K

H

Y

K

D

G

G

A

P

S

S

V

L

V

I

E

I

V

A

L

Y

Q

A

N

P

C
|
C

V

I

I

-

F

-

N

N

D

H

G

G

T

I

H

N

A

M

S

T

V

Y

A

S

T

Q

K

R

E

E

G

-

R

-

A

A

K

K

N

K

A

A

I

V

Y

-

L

-

E

E

H

A

G

G

K

Y

P

W

M

P

L

L

F

Y

G

R

E

Y

N

N

K

P

E

Q

F

-

G

-

L

L

M

A

Q

Q

E

E

G

G

D967 (= D95) binding Mg(2+)
DGW 967:969 (≠ DGD 95:97) binding thiamine diphosphate
T995 (≠ N123) binding Mg(2+)
TEVYSN 995:1000 (≠ NRIYGL 123:128) binding thiamine diphosphate
V997 (≠ I125) binding Mg(2+)
C1075 (= C202) binding [4Fe-4S] cluster

Sites not aligning to the query:

424:428 binding CoA
456 binding CoA
556 binding CoA
598 binding CoA
686 binding [4Fe-4S] cluster
689 binding [4Fe-4S] cluster
692 binding [4Fe-4S] cluster
696 binding [4Fe-4S] cluster
742 binding [4Fe-4S] cluster
745 binding [4Fe-4S] cluster
748 binding [4Fe-4S] cluster
752 binding [4Fe-4S] cluster
809 binding [4Fe-4S] cluster
812 binding [4Fe-4S] cluster
814 binding thiamine diphosphate
837 binding [4Fe-4S] cluster; binding thiamine diphosphate

6ciqA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with coenzyme a bound (see paper)
27% identity, 47% coverage: 88:246/336 of query aligns to 959:1110/1169 of 6ciqA

query
sites
6ciqA

T

S

I

I

W

W

Q

I

I

I

S

G

G
|
G

D
|
D

G
|
G

D

W

G

A

L

Y

A

D

I

I

G

G

G

Y

N

G

H

G

F

L

I

D

H

H

A

V

V

L

R

A

R

S

N

G

I

A

D

N

L

V

N

N

M

V

I

L

L

V

L

L

N

D

N
x
T

R

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

Y

S

S

S

P

K

T

A

S

T

E

Q

R

T

G

G

F

A

V

V

S

A

K

R

S

F

S

A

P

A

Y

G

G

G

T

K

V

F

E

T

D

K

P

K

F

K

H

D

P

L

A

G

E

L

L

M

A

A

F

M

G

-

A

S

R

Y

G

G

R

Y

F

V

F

Y

A

V

R

A

C

S

I

V

A

A

V

M

D

-

G

G

A

A

A

S

S

H

V

S

E

Q

V

L

L

M

K

K

A

A

-

L

-

I

-

E

A

A

A

E

N

K

H

Y

K

D

G

G

A

P

S

S

V

L

V

I

E

I

V

A

L

Y

Q

A

N

P

C
|
C

V
x
I

I

-

F

-

N

N

D

H

G

G

T

I

H

N

A

M

S

T

V

Y

A

S

T

Q

K

R

E

E

G

-

R

-

A

A

K

K

N

K

A

A

I

V

Y

-

L

-

E

E

H

A

G

G

K

Y

P

W

M

P

L

L

F

Y

G

R

E

Y

N

N

K

P

E

Q

F

-

G

-

L

L

M

A

Q

Q

E

E

G

G

active site: N999 (≠ L128)
binding coenzyme a: N999 (≠ L128)
binding magnesium ion: D966 (= D95), T994 (≠ N123), V996 (≠ I125)
binding iron/sulfur cluster: S998 (≠ G127), C1074 (= C202), I1075 (≠ V203)
binding thiamine diphosphate: G965 (= G94), D966 (= D95), G967 (= G96), V996 (≠ I125), Y997 (= Y126), S998 (≠ G127), N999 (≠ L128), T1000 (= T129)

Sites not aligning to the query:

active site: 28, 61, 111
binding coenzyme a: 28, 423, 424, 427, 428, 553, 586
binding iron/sulfur cluster: 678, 680, 685, 686, 688, 689, 691, 695, 696, 700, 734, 741, 742, 743, 744, 745, 747, 751, 757, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 85, 813, 835, 836, 865

6cipA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with acetyl-tpp bound (see paper)
27% identity, 47% coverage: 88:246/336 of query aligns to 959:1110/1165 of 6cipA

query
sites
6cipA

T

S

I

I

W

W

Q

I

I

I

S

G

G
|
G

D
|
D

G
|
G

D

W

G

A

L

Y

A

D

I

I

G

G

G

Y

N

G

H

G

F

L

I

D

H

H

A

V

V

L

R

A

R

S

N

G

I

A

D

N

L

V

N

N

M

V

I

L

L

V

L

L

N

D

N
x
T

R

E

I
x
V

Y

Y

G
x
S

L
x
N

T

T

K

G

G

G

Q

Q

Y

S

S

S

P

K

T

A

S

T

E

Q

R

T

G

G

F

A

V

V

S

A

K

R

S

F

S

A

P

A

Y

G

G

G

T

K

V

F

E

T

D

K

P

K

F

K

H

D

P

L

A

G

E

L

L

M

A

A

F

M

G

-

A

S

R

Y

G

G

R

Y

F

V

F

Y

A

V

R

A

C

S

I

V

A

A

V

M

D

-

G

G

A

A

A

S

S

H

V

S

E

Q

V

L

L

M

K

K

A

A

-

L

-

I

-

E

A

A

A

E

N

K

H

Y

K

D

G

G

A

P

S

S

V

L

V

I

E

I

V

A

L

Y

Q

A

N

P

C
|
C

V

I

I

-

F

-

N

N

D

H

G

G

T

I

H

N

A

M

S

T

V

Y

A

S

T

Q

K

R

E

E

G

-

R

-

A

A

K

K

N

K

A

A

I

V

Y

-

L

-

E

E

H

A

G

G

K

Y

P

W

M

P

L

L

F

Y

G

R

E

Y

N

N

K

P

E

Q

F

-

G

-

L

L

M

A

Q

Q

E

E

G

G

active site: N999 (≠ L128)
binding 2-acetyl-thiamine diphosphate: G965 (= G94), D966 (= D95), G967 (= G96), V996 (≠ I125), S998 (≠ G127), N999 (≠ L128)
binding magnesium ion: D966 (= D95), T994 (≠ N123), V996 (≠ I125)
binding iron/sulfur cluster: S998 (≠ G127), C1074 (= C202)

Sites not aligning to the query:

active site: 28, 61, 111
binding 2-acetyl-thiamine diphosphate: 27, 61, 85, 111, 813, 834, 835, 836, 865
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836

6cinB Crystal structure of pyruvate:ferredoxin oxidoreductase from moorella thermoacetica (see paper)
27% identity, 47% coverage: 88:246/336 of query aligns to 959:1110/1169 of 6cinB

query
sites
6cinB

T

S

I

I

W

W

Q

I

I

I

S

G

G
|
G

D
|
D

G
|
G

D

W

G

A

L

Y

A

D

I

I

G

G

G

Y

N

G

H

G

F

L

I

D

H

H

A

V

V

L

R

A

R

S

N

G

I

A

D

N

L

V

N

N

M

V

I

L

L

V

L

L

N

D

N
x
T

R

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

Y

S

S

S

P

K

T

A

S

T

E

Q

R

T

G

G

F

A

V

V

S

A

K

R

S

F

S

A

P

A

Y

G

G

G

T

K

V

F

E

T

D

K

P

K

F

K

H

D

P

L

A

G

E

L

L

M

A

A

F

M

G

-

A

S

R

Y

G

G

R

Y

F

V

F

Y

A

V

R

A

C

S

I

V

A

A

V

M

D

-

G

G

A

A

A

S

S

H

V

S

E

Q

V

L

L

M

K

K

A

A

-

L

-

I

-

E

A

A

A

E

N

K

H

Y

K

D

G

G

A

P

S

S

V

L

V

I

E

I

V

A

L

Y

Q

A

N

P

C
|
C

V
x
I

I

-

F

-

N

N

D

H

G

G

T

I

H

N

A

M

S

T

V

Y

A

S

T

Q

K

R

E

E

G

-

R

-

A

A

K

K

N

K

A

A

I

V

Y

-

L

-

E

E

H

A

G

G

K

Y

P

W

M

P

L

L

F

Y

G

R

E

Y

N

N

K

P

E

Q

F

-

G

-

L

L

M

A

Q

Q

E

E

G

G

active site: N999 (≠ L128)
binding magnesium ion: D966 (= D95), T994 (≠ N123), V996 (≠ I125)
binding iron/sulfur cluster: S998 (≠ G127), C1074 (= C202), I1075 (≠ V203)
binding thiamine diphosphate: G965 (= G94), D966 (= D95), G967 (= G96), V996 (≠ I125), Y997 (= Y126), S998 (≠ G127), N999 (≠ L128), T1000 (= T129)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 685, 686, 687, 688, 689, 691, 695, 700, 741, 742, 743, 744, 745, 746, 747, 751, 752, 757, 758, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 834, 835, 836, 865

Query Sequence

>352364 FitnessBrowser__Btheta:352364
MSDKVYTVQDYKSGQPRWCPGCGDHAFLNSLHKAMAELGVAPHNIAVISGIGCSSRLPYY
VNTYGFHTIHGRAAAVATGAKVANPDLTIWQISGDGDGLAIGGNHFIHAVRRNIDLNMIL
LNNRIYGLTKGQYSPTSERGFVSKSSPYGTVEDPFHPAELAFGARGRFFARCIAVDGAAS
VEVLKAAANHKGASVVEVLQNCVIFNDGTHASVATKEGRAKNAIYLEHGKPMLFGENKEF
GLMQEGFGLKVVKLGENGITEKDILIHDAHCQDNTLQLKLALMEGPDFPIALGVIREVEA
PTYNDAVAEQIEEVKGKKKYHNFQELLMTNDTWEVK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory