SitesBLAST

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
44% identity, 63% coverage: 18:228/336 of query aligns to 11:218/304 of Q96XT4

query
sites
Q96XT4

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

D

H

F

A

G

F

I

L

L

N

R

S

A

L

E

H

E

K

M

A

A

M

I

A

R

E

E

L

L

G

G

V

I

A

N

P

P

H

K

N

S

I

V

A

V

V

I

I

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

S

G

R

K

L

I

P

P

Y

H

Y

F

V

M

N

N

-

L

-

P

T

I

Y

S

G

G

F

V

H

H

T

T

I

L

H

H

G

G

R

R

A

S

A

I

A

A

V

F

A

A

T

T

G

G

A

I

K

K

V

L

A

S

N

N

P

P

D

S

L

L

T

E

I

V

W

I

Q

V

I

N

S

V

G

G

D
|
D

G
|
G

D
|
D

G

G

L

L

A

G

I

I

G

G

G

M

N

G

H

H

F

F

I

V

H

H

A

L

V

G

R

R

N

N

I

I

D

D

L

I

N

A

M

V

I

L

L

V

L

H

N

N

N
|
N

R

G

I
x
V

Y

Y

G
|
G

L
|
L

T

T

K

K

G

G

Q

Q

Y

A

S

S

P

P

T

T

S

L

E

H

R

R

G

G

F

E

V

K

S

T

K

K

S

S

S

L

P

P

Y

K

G

P

T

N

V

I

E

M

D

D

P

A

F

V

H

N

P

P

A

L

E

A

L

V

A

A

F

L

G

A

A

A

R

G

G

Y

R

T

F

F

F

V

A

A

R

R

C

G

I

Y

A

A

V

Y

D

D

G

V

A

M

A

H

S

L

V

K

E

E

V

L

L

I

K

K

A

K

A

A

A

I

N

L

H

H

K

K

G

G

A

S

S

A

V

L

V

V

E

D

V

I

L

L

Q

Q

N

P

C
|
C

V

P

I

T

F

Y

N

N

D

D

G

-

T

-

H

-

A

-

S

-

V

I

A

N

T

T

K

K

E

E

G

W

R

Y

A

D

K

K

N

R

A

V

I

Y

Y

K

L

L

E

D

H

N

C12 (= C19) binding
C15 (= C22) binding
IGCS 44:47 (= IGCS 51:54) binding
C46 (= C53) binding
D90 (= D95) binding
GD 91:92 (= GD 96:97) binding
N118 (= N123) binding
V120 (≠ I125) binding
GL 122:123 (= GL 127:128) binding
C197 (= C202) binding

5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
44% identity, 63% coverage: 18:228/336 of query aligns to 8:215/301 of 5b46B

query
sites
5b46B

W
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W

C
|
C

P

P

G

G

C
|
C

G

G

D

D

H

F

A

G

F

I

L

L

N

R

S

A

L

E

H

E

K

M

A

A

M

I

A

R

E

E

L

L

G

G

V

I

A

N

P

P

H

K

N

S

I

V

A

V

V

I

I

V

S

S

G

G

I
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I

G
|
G

C
|
C

S
|
S

S

G

R

K

L

I

P

P

Y

H

Y

F

V

M

N

N

-

L

-

P

T

I

Y

S

G

G

F

V

H

H

T

T

I

L

H
|
H

G

G

R

R

A

S

A

I

A

A

V

F

A

A

T

T

G

G

A

I

K

K

V

L

A

S

N

N

P

P

D

S

L

L

T

E

I

V

W

I

Q

V

I

N

S

V

G
|
G

D
|
D

G
|
G

D
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D

G

G

L

L

A

G

I

I

G

G

G

M

N

G

H

H

F

F

I

V

H

H

A

L

V

G

R

R

N

N

I

I

D

D

L

I

N

A

M

V

I

L

L

V

L

H

N

N

N
|
N

R

G

I
x
V

Y
|
Y

G
|
G

L
|
L

T
|
T

K

K

G

G

Q

Q

Y

A

S

S

P

P

T

T

S

L

E

H

R

R

G

G

F

E

V

K

S

T

K

K

S

S

S

L

P

P

Y

K

G

P

T

N

V

I

E

M

D

D

P

A

F

V

H

N

P

P

A

L

E

A

L

V

A

A

F

L

G

A

A

A

R

G

G

Y

R

T

F

F

F

V

A

A

R

R

C

G

I

Y

A

A

V

Y

D

D

G

V

A

M

A

H

S

L

V

K

E

E

V

L

L

I

K

K

A

K

A

A

A

I

N

L

H

H

K

K

G

G

A

S

S

A

V

L

V

V

E

D

V

I

L

L

Q

Q

N

P

C
|
C

V

P

I
x
T

F
x
Y

N

N

D

D

G

-

T

-

H

-

A

-

S

-

V

I

A

N

T

T

K

K

E

E

G

W

R

Y

A

D

K

K

N

R

A

V

I

Y

Y

K

L

L

E

D

H

N

binding magnesium ion: D87 (= D95), N115 (= N123), V117 (≠ I125)
binding iron/sulfur cluster: W8 (= W18), C9 (= C19), C12 (= C22), C43 (= C53), C194 (= C202), T196 (≠ I204), Y197 (≠ F205)
binding thiamine diphosphate: I41 (= I51), G42 (= G52), C43 (= C53), S44 (= S54), H62 (= H70), G86 (= G94), G88 (= G96), D89 (= D97), N115 (= N123), V117 (≠ I125), Y118 (= Y126), G119 (= G127), L120 (= L128), T121 (= T129)

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
46% identity, 63% coverage: 18:227/336 of query aligns to 11:217/305 of P72579

query
sites
P72579

W

W

C

C

P

P

G

G

C

C

G

G

D

N

H

F

A

G

F

I

L

L

N

N

S

A

L

E

H

Q

K

Q

A

A

M

I

A

V

E

E

L

L

G

G

V

V

A

D

P

T

H

K

N

N

I

V

A

V

V

V

I

V

S

S

G

G

I

I

G

G

C

C

S

S

S

G

R
x
K

L

I

P

P

Y

H

Y

F

V

F

N

R

T

T

-

P

-

I

Y

S

G

G

F

V

H

H

T

T

I

L

H

H

G

G

R

R

A

A

A

I

A

A

V

F

A

A

T

T

G

G

A

I

K

K

V

L

A

S

N

N

P

P

D

D

L

L

T

V

I

V

W

I

Q

V

I

N

S

G

G

G

D

D

G

G

D

D

G

L

L

L

A

G

I

I

G

G

G

A

N

G

H

H

F

F

I

V

H

A

A

A

V

G

R

R

N

N

I

V

D

D

L

M

N

V

M

V

I

I

L

L

L

H

N

D

N

N

R

G

I

V

Y

Y

G

G

L
|
L

T

T

K

K

G

G

Q

Q

Y

A

S

S

P

P

T

T

S

L

E

K

R

R

G

G

F

E

V

K

S

P

K

K

S

S

S

L

P

P

Y

R

G

P

T

N

V

I

E

N

D

D

P

A

F

V

H

N

P

P

A

I

E

A

L

L

A

A

F

I

G

S

A

S

R

G

G

Y

R

T

F

F

F

V

A

A

R

R

C

G

I

Y

A

A

V

Y

D

D

G

V

A

K

A

H

S

L

V

K

E

E

V

L

L

I

K

K

A

S

A

A

A

I

N

K

H

H

K

K

G

G

A

L

S

A

V

L

V

I

E

D

V

V

L

L

Q

Q

N

P

C

C

V

P

I

T

F

Y

N

N

D

D

G

-

T

-

H

-

A

-

S

-

V

I

A

N

T

T

K

K

E

E

G

W

R

Y

A

D

K

K

N

R

A

I

I

Y

Y

K

L

L

E

D

K49 (≠ R56) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (= L128) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
46% identity, 63% coverage: 18:227/336 of query aligns to 11:217/305 of Q96Y68

query
sites
Q96Y68

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

N

H

F

A

G

F

I

L

L

N

N

S

A

L

E

H

Q

K

Q

A

A

M

I

A

V

E

E

L

L

G

G

V

V

A

D

P

T

H

K

N

N

I

V

A

V

V

V

I

V

S

S

G

G

I

I

G

G

C
|
C

S

S

S

G

R
x
K

L

I

P

P

Y

H

Y

F

V

F

N

R

T

T

-

P

-

I

Y

S

G

G

F

V

H

H

T

T

I

L

H

H

G

G

R

R

A

A

A

I

A

A

V

F

A

A

T

T

G

G

A

I

K

K

V

L

A

S

N

N

P

P

D

D

L

L

T

V

I

V

W

I

Q

V

I

N

S

G

G

G

D
|
D

G
|
G

D
|
D

G

L

L

L

A

G

I

I

G

G

G

A

N

G

H

H

F

F

I

V

H

A

A

A

V

G

R

R

N

N

I

V

D

D

L

M

N

V

M

V

I

I

L

L

L

H

N

D

N
|
N

R

G

I
x
V

Y

Y

G
|
G

L
|
L

T

T

K
|
K

G

G

Q

Q

Y

A

S

S

P

P

T

T

S

L

E

K

R

R

G

G

F

E

V

K

S

P

K

K

S

S

S

L

P

P

Y

R

G

P

T

N

V

I

E

N

D

D

P

A

F

V

H

N

P

P

A

I

E

A

L

L

A

A

F

I

G

S

A

S

R

G

G

Y

R

T

F

F

F

V

A

A

R

R

C

G

I

Y

A

A

V

Y

D

D

G

V

A
x
K

A

H

S

L

V

K

E

E

V

L

L

I

K

K

A

S

A

A

A

I

N

K

H

H

K

K

G

G

A

L

S

A

V

L

V

I

E

D

V

V

L

L

Q

Q

N

P

C
|
C

V

P

I

T

F

Y

N

N

D

D

G

-

T

-

H

-

A

-

S

-

V

I

A

N

T

T

K

K

E

E

G

W

R

Y

A

D

K

K

N

R

A

I

I

Y

Y

K

L

L

E

D

C12 (= C19) binding
C15 (= C22) binding
C46 (= C53) binding
K49 (≠ R56) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D95) binding
GD 91:92 (= GD 96:97) binding
N118 (= N123) binding
V120 (≠ I125) binding
GL 122:123 (= GL 127:128) binding
K125 (= K130) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (≠ A178) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C202) binding

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
44% identity, 64% coverage: 18:233/336 of query aligns to 7:209/286 of 5b48B

query
sites
5b48B

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

N

H

F

A

G

F

I

L

L

N

N

S

A

L

E

H

Q

K

Q

A

A

M

I

A

V

E

E

L

L

G

G

V

V

A

D

P

T

H

K

N

N

I

V

A

V

V

V

I

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

S

G

R

K

L

I

P

P

Y

H

Y

F

V

T

N

P

T

I

Y

S

G

G

F

V

H

H

T

T

I

L

H
|
H

G

G

R

R

A

A

A

I

A

A

V

F

A

A

T

T

G

G

A

I

K

K

V

L

A

S

N

N

P

P

D

D

L

L

T

V

I

V

W

I

Q

V

I

N

S

G

G

G

D
|
D

G
|
G

D
|
D

G

L

L

L

A

G

I

I

G

G

G

A

N

G

H

H

F

F

I

V

H

A

A

A

V

G

R

R

N

N

I

V

D

D

L

M

N

V

M

V

I

I

L

L

L

H

N

D

N
|
N

R

G

I
x
V

Y
|
Y

G
|
G

L
|
L

T

T

K

K

G

G

Q

Q

Y

A

S

S

P

P

T

T

S

L

E

K

R

R

G

G

F

-

V

-

S

-

K

-

S

-

P

-

Y

-

G

E

T

N

V

I

E

N

D

D

P

A

F

V

H

N

P

P

A

I

E

A

L

L

A

A

F

I

G

S

A

S

R

G

G

Y

R

T

F

F

F

V

A

A

R

R

C

G

I

Y

A

A

V

Y

D

D

G

V

A

K

A

H

S

L

V

K

E

E

V

L

L

I

K

K

A

S

A

A

A

I

N

K

H

H

K

K

G

G

A

L

S

A

V

L

V

I

E

D

V

V

L

L

Q

Q

N

P

C
|
C

V

P

I
x
T

F

Y

N

N

D

D

G

-

T

-

H

-

A

-

S

-

V

I

A

N

T

T

K

K

E

E

G

W

R

R

A

I

K

Y

N

K

A

L

I

D

Y

T

L

L

E

P

H

D

G

W

K

D

P

P

M

V

L

V

binding magnesium ion: D84 (= D95), N112 (= N123), V114 (≠ I125)
binding iron/sulfur cluster: C8 (= C19), C11 (= C22), C42 (= C53), C183 (= C202), T185 (≠ I204)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I51), G41 (= G52), C42 (= C53), S43 (= S54), H59 (= H70), G85 (= G96), D86 (= D97), N112 (= N123), V114 (≠ I125), Y115 (= Y126), G116 (= G127), L117 (= L128)

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
43% identity, 55% coverage: 18:202/336 of query aligns to 4:179/275 of 5b47B

query
sites
5b47B

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

D

H

F

A

G

F

I

L

L

N

R

S

A

L

E

H

E

K

M

A

A

M

I

A

R

E

E

L

L

G

G

V

I

A

N

P

P

H

K

N

S

I

V

A

V

V

I

I

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

S

G

R

K

L

I

P

P

Y

H

Y

F

V

M

N

N

-

L

-

P

T

I

Y

S

G

G

F

V

H

H

T

T

I

L

H
|
H

G

G

R

R

A

S

A

I

A

A

V

F

A

A

T

T

G

G

A

I

K

K

V

L

A

S

N

N

P

P

D

S

L

L

T

E

I

V

W

I

Q

V

I

N

S

V

G

G

D
|
D

G
|
G

D
|
D

G

G

L

L

A

G

I

I

G

G

G

M

N

G

H

H

F

F

I

V

H

H

A

L

V

G

R

R

N

N

I

I

D

D

L

I

N

A

M

V

I

L

L

V

L

H

N

N

N
|
N

R

G

I
x
V

Y
|
Y

G

G

L
|
L

T

-

K

-

G

-

Q

Q

Y

A

S

S

P

P

T

T

S

L

E

H

R

R

G

G

F

-

V

-

S

-

K

-

S

-

P

-

Y

-

G

E

T

N

V

I

E

M

D

D

P

A

F

V

H

N

P

P

A

L

E

A

L

V

A

A

F

L

G

A

A

A

R

G

G

Y

R

T

F

F

F

V

A

A

R

R

C

G

I

Y

A

A

V

Y

D

D

G

V

A

M

A

H

S

L

V

K

E

E

V

L

L

I

K

K

A

K

A

A

A

I

N

L

H

H

K

K

G

G

A

S

S

A

V

L

V

V

E

D

V

I

L

L

Q

Q

N

P

C
|
C

binding magnesium ion: D83 (= D95), N111 (= N123)
binding iron/sulfur cluster: C5 (= C19), C8 (= C22), C39 (= C53), C179 (= C202)
binding thiamine diphosphate: I37 (= I51), G38 (= G52), C39 (= C53), S40 (= S54), H58 (= H70), G84 (= G96), D85 (= D97), N111 (= N123), V113 (≠ I125), Y114 (= Y126), L116 (= L128)

6cioA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with lactyl-tpp bound (see paper)
27% identity, 47% coverage: 88:246/336 of query aligns to 959:1110/1164 of 6cioA

query
sites
6cioA

T

S

I

I

W

W

Q

I

I

I

S

G

G
|
G

D
|
D

G
|
G

D

W

G

A

L

Y

A

D

I

I

G

G

G

Y

N

G

H

G

F

L

I

D

H

H

A

V

V

L

R

A

R

S

N

G

I

A

D

N

L

V

N

N

M

V

I

L

L

V

L

L

N

D

N
x
T

R

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

Y

S

S

S

P

K

T

A

S

T

E

Q

R

T

G

G

F

A

V

V

S

A

K

R

S

F

S

A

P

A

Y

G

G

G

T

K

V

F

E

T

D

K

P

K

F

K

H

D

P

L

A

G

E

L

L

M

A

A

F

M

G

-

A

S

R

Y

G

G

R

Y

F

V

F

Y

A

V

R

A

C

S

I

V

A

A

V

M

D

-

G

G

A

A

A

S

S

H

V

S

E

Q

V

L

L

M

K

K

A

A

-

L

-

I

-

E

A

A

A

E

N

K

H

Y

K

D

G

G

A

P

S

S

V

L

V

I

E

I

V

A

L

Y

Q

A

N

P

C
|
C

V
x
I

I

-

F

-

N

N

D

H

G

G

T

I

H

N

A

M

S

T

V

Y

A

S

T

Q

K

R

E

E

G

-

R

-

A

A

K

K

N

K

A

A

I

V

Y

-

L

-

E

E

H

A

G

G

K

Y

P

W

M

P

L

L

F

Y

G

R

E

Y

N

N

K

P

E

Q

F

-

G

-

L

L

M

A

Q

Q

E

E

G

G

active site: N999 (≠ L128)
binding magnesium ion: D966 (= D95), T994 (≠ N123), V996 (≠ I125)
binding iron/sulfur cluster: C1074 (= C202), I1075 (≠ V203)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: G965 (= G94), D966 (= D95), G967 (= G96), V996 (≠ I125), Y997 (= Y126), S998 (≠ G127), N999 (≠ L128), T1000 (= T129)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 26, 27, 28, 61, 85, 111, 813, 834, 835, 836, 865

Q2RMD6 Pyruvate:ferredoxin oxidoreductase; PFOR; Pyruvate synthase; EC 1.2.7.1 from Moorella thermoacetica (strain ATCC 39073 / JCM 9320) (see paper)
27% identity, 47% coverage: 88:246/336 of query aligns to 960:1111/1171 of Q2RMD6

query
sites
Q2RMD6

T

S

I

I

W

W

Q

I

I

I

S

G

G

G

D
|
D

G
|
G

D
x
W

G

A

L

Y

A

D

I

I

G

G

G

Y

N

G

H

G

F

L

I

D

H

H

A

V

V

L

R

A

R

S

N

G

I

A

D

N

L

V

N

N

M

V

I

L

L

V

L

L

N

D

N
x
T

R
x
E

I
x
V

Y
|
Y

G
x
S

L
x
N

T

T

K

G

G

G

Q

Q

Y

S

S

S

P

K

T

A

S

T

E

Q

R

T

G

G

F

A

V

V

S

A

K

R

S

F

S

A

P

A

Y

G

G

G

T

K

V

F

E

T

D

K

P

K

F

K

H

D

P

L

A

G

E

L

L

M

A

A

F

M

G

-

A

S

R

Y

G

G

R

Y

F

V

F

Y

A

V

R

A

C

S

I

V

A

A

V

M

D

-

G

G

A

A

A

S

S

H

V

S

E

Q

V

L

L

M

K

K

A

A

-

L

-

I

-

E

A

A

A

E

N

K

H

Y

K

D

G

G

A

P

S

S

V

L

V

I

E

I

V

A

L

Y

Q

A

N

P

C
|
C

V

I

I

-

F

-

N

N

D

H

G

G

T

I

H

N

A

M

S

T

V

Y

A

S

T

Q

K

R

E

E

G

-

R

-

A

A

K

K

N

K

A

A

I

V

Y

-

L

-

E

E

H

A

G

G

K

Y

P

W

M

P

L

L

F

Y

G

R

E

Y

N

N

K

P

E

Q

F

-

G

-

L

L

M

A

Q

Q

E

E

G

G

D967 (= D95) binding
DGW 967:969 (≠ DGD 95:97) binding
T995 (≠ N123) binding
TEVYSN 995:1000 (≠ NRIYGL 123:128) binding
V997 (≠ I125) binding
C1075 (= C202) binding

Sites not aligning to the query:

424:428 binding
456 binding
556 binding
598 binding
686 binding
689 binding
692 binding
696 binding
742 binding
745 binding
748 binding
752 binding
809 binding
812 binding
814 binding
837 binding ; binding

6ciqA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with coenzyme a bound (see paper)
27% identity, 47% coverage: 88:246/336 of query aligns to 959:1110/1169 of 6ciqA

query
sites
6ciqA

T

S

I

I

W

W

Q

I

I

I

S

G

G
|
G

D
|
D

G
|
G

D

W

G

A

L

Y

A

D

I

I

G

G

G

Y

N

G

H

G

F

L

I

D

H

H

A

V

V

L

R

A

R

S

N

G

I

A

D

N

L

V

N

N

M

V

I

L

L

V

L

L

N

D

N
x
T

R

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

Y

S

S

S

P

K

T

A

S

T

E

Q

R

T

G

G

F

A

V

V

S

A

K

R

S

F

S

A

P

A

Y

G

G

G

T

K

V

F

E

T

D

K

P

K

F

K

H

D

P

L

A

G

E

L

L

M

A

A

F

M

G

-

A

S

R

Y

G

G

R

Y

F

V

F

Y

A

V

R

A

C

S

I

V

A

A

V

M

D

-

G

G

A

A

A

S

S

H

V

S

E

Q

V

L

L

M

K

K

A

A

-

L

-

I

-

E

A

A

A

E

N

K

H

Y

K

D

G

G

A

P

S

S

V

L

V

I

E

I

V

A

L

Y

Q

A

N

P

C
|
C

V
x
I

I

-

F

-

N

N

D

H

G

G

T

I

H

N

A

M

S

T

V

Y

A

S

T

Q

K

R

E

E

G

-

R

-

A

A

K

K

N

K

A

A

I

V

Y

-

L

-

E

E

H

A

G

G

K

Y

P

W

M

P

L

L

F

Y

G

R

E

Y

N

N

K

P

E

Q

F

-

G

-

L

L

M

A

Q

Q

E

E

G

G

active site: N999 (≠ L128)
binding coenzyme a: N999 (≠ L128)
binding magnesium ion: D966 (= D95), T994 (≠ N123), V996 (≠ I125)
binding iron/sulfur cluster: S998 (≠ G127), C1074 (= C202), I1075 (≠ V203)
binding thiamine diphosphate: G965 (= G94), D966 (= D95), G967 (= G96), V996 (≠ I125), Y997 (= Y126), S998 (≠ G127), N999 (≠ L128), T1000 (= T129)

Sites not aligning to the query:

active site: 28, 61, 111
binding coenzyme a: 28, 423, 424, 427, 428, 553, 586
binding iron/sulfur cluster: 678, 680, 685, 686, 688, 689, 691, 695, 696, 700, 734, 741, 742, 743, 744, 745, 747, 751, 757, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 85, 813, 835, 836, 865

6cipA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with acetyl-tpp bound (see paper)
27% identity, 47% coverage: 88:246/336 of query aligns to 959:1110/1165 of 6cipA

query
sites
6cipA

T

S

I

I

W

W

Q

I

I

I

S

G

G
|
G

D
|
D

G
|
G

D

W

G

A

L

Y

A

D

I

I

G

G

G

Y

N

G

H

G

F

L

I

D

H

H

A

V

V

L

R

A

R

S

N

G

I

A

D

N

L

V

N

N

M

V

I

L

L

V

L

L

N

D

N
x
T

R

E

I
x
V

Y

Y

G
x
S

L
x
N

T

T

K

G

G

G

Q

Q

Y

S

S

S

P

K

T

A

S

T

E

Q

R

T

G

G

F

A

V

V

S

A

K

R

S

F

S

A

P

A

Y

G

G

G

T

K

V

F

E

T

D

K

P

K

F

K

H

D

P

L

A

G

E

L

L

M

A

A

F

M

G

-

A

S

R

Y

G

G

R

Y

F

V

F

Y

A

V

R

A

C

S

I

V

A

A

V

M

D

-

G

G

A

A

A

S

S

H

V

S

E

Q

V

L

L

M

K

K

A

A

-

L

-

I

-

E

A

A

A

E

N

K

H

Y

K

D

G

G

A

P

S

S

V

L

V

I

E

I

V

A

L

Y

Q

A

N

P

C
|
C

V

I

I

-

F

-

N

N

D

H

G

G

T

I

H

N

A

M

S

T

V

Y

A

S

T

Q

K

R

E

E

G

-

R

-

A

A

K

K

N

K

A

A

I

V

Y

-

L

-

E

E

H

A

G

G

K

Y

P

W

M

P

L

L

F

Y

G

R

E

Y

N

N

K

P

E

Q

F

-

G

-

L

L

M

A

Q

Q

E

E

G

G

active site: N999 (≠ L128)
binding 2-acetyl-thiamine diphosphate: G965 (= G94), D966 (= D95), G967 (= G96), V996 (≠ I125), S998 (≠ G127), N999 (≠ L128)
binding magnesium ion: D966 (= D95), T994 (≠ N123), V996 (≠ I125)
binding iron/sulfur cluster: S998 (≠ G127), C1074 (= C202)

Sites not aligning to the query:

active site: 28, 61, 111
binding 2-acetyl-thiamine diphosphate: 27, 61, 85, 111, 813, 834, 835, 836, 865
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836

6cinB Crystal structure of pyruvate:ferredoxin oxidoreductase from moorella thermoacetica (see paper)
27% identity, 47% coverage: 88:246/336 of query aligns to 959:1110/1169 of 6cinB

query
sites
6cinB

T

S

I

I

W

W

Q

I

I

I

S

G

G
|
G

D
|
D

G
|
G

D

W

G

A

L

Y

A

D

I

I

G

G

G

Y

N

G

H

G

F

L

I

D

H

H

A

V

V

L

R

A

R

S

N

G

I

A

D

N

L

V

N

N

M

V

I

L

L

V

L

L

N

D

N
x
T

R

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

Y

S

S

S

P

K

T

A

S

T

E

Q

R

T

G

G

F

A

V

V

S

A

K

R

S

F

S

A

P

A

Y

G

G

G

T

K

V

F

E

T

D

K

P

K

F

K

H

D

P

L

A

G

E

L

L

M

A

A

F

M

G

-

A

S

R

Y

G

G

R

Y

F

V

F

Y

A

V

R

A

C

S

I

V

A

A

V

M

D

-

G

G

A

A

A

S

S

H

V

S

E

Q

V

L

L

M

K

K

A

A

-

L

-

I

-

E

A

A

A

E

N

K

H

Y

K

D

G

G

A

P

S

S

V

L

V

I

E

I

V

A

L

Y

Q

A

N

P

C
|
C

V
x
I

I

-

F

-

N

N

D

H

G

G

T

I

H

N

A

M

S

T

V

Y

A

S

T

Q

K

R

E

E

G

-

R

-

A

A

K

K

N

K

A

A

I

V

Y

-

L

-

E

E

H

A

G

G

K

Y

P

W

M

P

L

L

F

Y

G

R

E

Y

N

N

K

P

E

Q

F

-

G

-

L

L

M

A

Q

Q

E

E

G

G

active site: N999 (≠ L128)
binding magnesium ion: D966 (= D95), T994 (≠ N123), V996 (≠ I125)
binding iron/sulfur cluster: S998 (≠ G127), C1074 (= C202), I1075 (≠ V203)
binding thiamine diphosphate: G965 (= G94), D966 (= D95), G967 (= G96), V996 (≠ I125), Y997 (= Y126), S998 (≠ G127), N999 (≠ L128), T1000 (= T129)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 685, 686, 687, 688, 689, 691, 695, 700, 741, 742, 743, 744, 745, 746, 747, 751, 752, 757, 758, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 834, 835, 836, 865

Query Sequence

>352364 FitnessBrowser__Btheta:352364
MSDKVYTVQDYKSGQPRWCPGCGDHAFLNSLHKAMAELGVAPHNIAVISGIGCSSRLPYY
VNTYGFHTIHGRAAAVATGAKVANPDLTIWQISGDGDGLAIGGNHFIHAVRRNIDLNMIL
LNNRIYGLTKGQYSPTSERGFVSKSSPYGTVEDPFHPAELAFGARGRFFARCIAVDGAAS
VEVLKAAANHKGASVVEVLQNCVIFNDGTHASVATKEGRAKNAIYLEHGKPMLFGENKEF
GLMQEGFGLKVVKLGENGITEKDILIHDAHCQDNTLQLKLALMEGPDFPIALGVIREVEA
PTYNDAVAEQIEEVKGKKKYHNFQELLMTNDTWEVK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory