SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 352404 FitnessBrowser__Btheta:352404 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 63% coverage: 70:195/201 of query aligns to 48:184/203 of P07464

query
sites
P07464

E

E

L

S

I

L

V

I

K

K

G

E

N

M

F

F

D

A

I

T

I

V

G

G

S

E

T

N

V

A

V

W

V

V

L

E

P

P

D

P

A

V

K

Y

L

F

I
x
S

L

Y

G

G

S

S

G

N

-

I

-

H

-

I

-

G

-

R

-

N

-

F

Y

Y

I

A

N
|
N

F

F

H

N

S

L

K

T

L

I

H

V

C

D

F
x
D

N

Y

H

T

I

V

E

T

I

I

G

G

E

D

N

N

V

V

I

L

I

I

S

A

E

P

N

N

V

V

I

T

I

L

R

S

D

V

S

T

D

G

N
x
H

-

P

-

V

-

H

H

H

Q

E

I

L

T

R

G

K

G

N

N

G

S

E

M

M

F

Y

A

S

-

F

P

P

V

I

I

T

I

I

K

G

D

N

N

N

A

V

W

W

I

I

G

G

M
x
S

S

H

A

V

I

V

I

I

L

N

K

P

G

G

V

V

T

T

V

I

G

G

E

D

G

N

A

S

I

V

V

I

A

G

A
|
A

G

G

S

S

V

I

V

V

T
|
T

K
|
K

D

D

V

I

P

P

H

N

T

V

I

V

V

A

A

A

G

G

V

V

P

P

A

C

R
|
R

V

V

I

I

K
x
R

K

E

S71 (≠ I93) binding
N85 (= N100) binding in other chain; binding in other chain
D93 (≠ F108) binding
H115 (≠ N130) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ M153) binding in other chain
A160 (= A171) binding in other chain
TK 165:166 (= TK 176:177) binding
R180 (= R191) binding
R183 (≠ K194) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
34% identity, 63% coverage: 70:195/201 of query aligns to 47:183/201 of 1krvA

query
sites
1krvA

E

E

L

S

I

L

V

I

K

K

G

E

N

M

F

F

D

A

I

T

I

V

G

G

S

E

T

N

V

A

V

W

V

V

L

E

P

P

D

P

A

V

K

Y

L

F

I
x
S

L

Y

G

G

S

S

G

N

-

I

-

H

-

I

-

G

-

R

-

N

-

F

Y
|
Y

I

A

N
|
N

F

F

H

N

S

L

K

T

L

I

H
x
V

C

D

F
x
D

N

Y

H

T

I

V

E

T

I

I

G

G

E

D

N

N

V

V

I

L

I

I

S
x
A

E

P

N

N

V

V

I

T

I

L

R
x
S

D

V

S
x
T

D

G

N

H

-

P

-

V

-

H

H

H

Q

E

I

L

T

R

G

K

G

N

N

G

S

E

M
|
M

F

Y

A

S

-

F

P

P

V

I

I

T

I

I

K

G

D

N

N

N

A

V

W
|
W

I

I

G
|
G

M
x
S

S

H

A

V

I

V

I

I

L
x
N

K

P

G

G

V

V

T

T

V

I

G

G

E

D

G

N

A

S

I

V

V

I

A

G

A
|
A

G

G

S

S

V

I

V

V

T

T

K

K

D

D

V

I

P

P

H

N

T

V

I

V

V

A

A

A

G

G

V

V

P
|
P

A

C

R
|
R

V

V

I

I

K
x
R

K

E

binding 4-nitrophenyl beta-D-galactopyranoside: S70 (≠ I93), Y82 (= Y98), N84 (= N100), V90 (≠ H106), D92 (≠ F108), M126 (= M139)
binding coenzyme a: A104 (≠ S120), S110 (≠ R126), T112 (≠ S128), W138 (= W150), G140 (= G152), S141 (≠ M153), N146 (≠ L158), A159 (= A171), P177 (= P189), R179 (= R191), R182 (≠ K194)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
34% identity, 63% coverage: 70:195/201 of query aligns to 47:183/201 of 1kruA

query
sites
1kruA

E

E

L

S

I

L

V

I

K

K

G

E

N

M

F

F

D

A

I

T

I

V

G

G

S

E

T

N

V

A

V
x
W

V

V

L

E

P

P

D

P

A

V

K

Y

L

F

I
x
S

L

Y

G

G

S

S

G

N

-

I

-

H

-

I

-

G

-

R

-

N

-

F

Y
|
Y

I

A

N
|
N

F

F

H

N

S

L

K

T

L

I

H
x
V

C

D

F
x
D

N

Y

H

T

I

V

E

T

I

I

G

G

E

D

N

N

V

V

I
x
L

I

I

S

A

E

P

N

N

V

V

I

T

I

L

R

S

D

V

S

T

D

G

N
x
H

-

P

-

V

-

H

H

H

Q

E

I

L

T

R

G

K

G

N

N

G

S

E

M
|
M

F

Y

A

S

-

F

P

P

V

I

I

T

I

I

K

G

D

N

N

N

A

V

W
|
W

I

I

G
|
G

M
x
S

S

H

A

V

I

V

I

I

L

N

K

P

G

G

V

V

T

T

V

I

G

G

E

D

G

N

A

S

I

V

V

I

A
x
G

A
|
A

G

G

S

S

V

I

V

V

T

T

K

K

D

D

V

I

P

P

H

N

T

V

I

V

V

A

A

A

G

G

V

V

P
|
P

A

C

R

R

V

V

I

I

K
x
R

K

E

binding coenzyme a: H114 (≠ N130), W138 (= W150), G140 (= G152), S141 (≠ M153), G158 (≠ A170), A159 (= A171), P177 (= P189), R182 (≠ K194)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: W62 (≠ V85), S70 (≠ I93), Y82 (= Y98), Y82 (= Y98), N84 (= N100), V90 (≠ H106), D92 (≠ F108), L102 (≠ I118), H114 (≠ N130), M126 (= M139)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
34% identity, 63% coverage: 70:195/201 of query aligns to 47:183/200 of 1krrA

query
sites
1krrA

E

E

L

S

I

L

V

I

K

K

G

E

N

M

F

F

D

A

I

T

I

V

G

G

S

E

T

N

V

A

V

W

V

V

L

E

P

P

D

P

A

V

K

Y

L

F

I

S

L

Y

G

G

S

S

G

N

-

I

-

H

-

I

-

G

-

R

-

N

-

F

Y

Y

I

A

N
|
N

F

F

H

N

S

L

K

T

L

I

H

V

C

D

F

D

N

Y

H

T

I

V

E

T

I

I

G

G

E

D

N

N

V

V

I

L

I
|
I

S
x
A

E
x
P

N

N

V

V

I

T

I

L

R

S

D

V

S
x
T

D

G

N
x
H

-

P

-

V

-

H

H

H

Q

E

I

L

T

R

G

K

G

N

N

G

S

E

M

M

F

Y

A

S

-

F

P

P

V

I

I

T

I

I

K

G

D

N

N

N

A

V

W

W

I

I

G
|
G

M
x
S

S

H

A

V

I

V

I

I

L
x
N

K

P

G

G

V

V

T

T

V

I

G

G

E

D

G

N

A

S

I

V

V

I

A
x
G

A
|
A

G

G

S

S

V

I

V

V

T

T

K

K

D

D

V

I

P

P

H

N

T

V

I

V

V

A

A
|
A

G

G

V

V

P
|
P

A

C

R

R

V

V

I

I

K
x
R

K

E

binding acetyl coenzyme *a: N84 (= N100), I103 (= I119), A104 (≠ S120), P105 (≠ E121), T112 (≠ S128), H114 (≠ N130), G140 (= G152), S141 (≠ M153), N146 (≠ L158), G158 (≠ A170), A159 (= A171), A174 (= A186), P177 (= P189), R182 (≠ K194)

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
43% identity, 48% coverage: 100:195/201 of query aligns to 80:183/186 of 4isxA

query
sites
4isxA

N

N

F

F

H

F

S

A

K
x
N

L

Y

H

D

C

C

F

I

-

F

-

L

-

D

-

V

N

C

H

K

I

I

E

E

I

I

G

G

E

D

N

N

V

V

I

M

I

L

S
x
A

E

P

N

N

V

V

I

Q

I

I

R

Y

D

T

S

A

-

Y

-

H

-

P

-

I

D

D

N

A

H

Q

Q

L

I

R

T

N

G

S

G

G

N

I

S

E

M

Y

F

G

A

S

P

P

V

V

I

K

I

I

K

G

D

D

N

N

A

V

W
|
W

I

I

G
|
G

M
x
G

S

G

A

V

I

I

L

T

K

P

G

G

V

I

T

T

V

I

G

G

E

D

G

N

A

V

I

V

V

I

A

G

A
|
A

G

G

S

S

V

V

T

T

K

K

D

D

V

I

P

P

H

N

T

T

I

V

V

A

A
x
V

G

G

V

N

P

P

A

C

R

R

V

V

I

I

K
|
K

K

K

binding acetyl coenzyme *a: N84 (≠ K104), A104 (≠ S120), W138 (= W150), G140 (= G152), G141 (≠ M153), A159 (= A171), V174 (≠ A186), K182 (= K194)

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
37% identity, 49% coverage: 98:195/201 of query aligns to 82:183/190 of 5u2kA

query
sites
5u2kA

Y

Y

I

V

N

N

F

T

H

N

S

C

K

Y

L

F

H
x
M

C

D

F

G

N

G

H

Q

I

I

E

T

I

I

G

G

E

D

N

N

V

V

I

F

I

I

S

G

E

P

N

N

V

C

I

G

I

F

R
x
Y

D

T

S
x
A

D

T

N
x
H

-

P

-

L

-

N

-

F

H

H

Q

H

I

R

T

N

G

E

G

G

N

F

S

E

M

K

F

A

A

G

P

P

V

I

I

H

I

I

K

G

D

S

N

N

A

T

W

W

I

F

G

G

M

G

S

H

A

V

I

A

I

V

L
|
L

K

P

G

G

V

V

T

T

V

I

G

G

E

E

G

G

A

S

I

V

V

I

A

G

A

A

G

G

S

S

V

V

T
|
T

K
|
K

D

D

V

I

P

P

H

H

T

S

I

L

V

A

A

V

G

G

V

N

P

P

A

C

R

K

V

V

I

V

K

R

K

K

binding coenzyme a: M90 (≠ H106), Y110 (≠ R126), A112 (≠ S128), H114 (≠ N130), L146 (= L158), T164 (= T176), K165 (= K177)

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
34% identity, 67% coverage: 65:199/201 of query aligns to 151:283/307 of A1ADJ6

query
sites
A1ADJ6

L

I

H

H

A

K

N

N

S

S

E

K

L

-

I

-

V

I

K

K

G

G

N

-

F

-

D

D

I

I

I

V

-

A

-

T

-

K

G

G

S

S

T

K

V

V

V

I

L

-

P

-

D

G

A

R

K

R

L

T

I

T

L

I

G

G

S

A

G

G

Y

F

I

E

N

V

F

V

H

T

S

D

K

K

L

C

H

N

C

-

F

-

N

-

H

-

I

V

E

T

I

I

G

G

E

H

N

D

V

C

I

M

I

I

S

A

E

R

N

D

V

V

I

I

I

L

R

R

D

A

S

S

D

D

N

G

H
|
H

Q

P

I

I

T

F

G

D

G

I

N

H

S

S

M

K

-

K

-

R

-

I

-

N

-

W

F

A

A

K

P

D

V

I

I

I

K

S

D

S

N

Y

A

V

W
|
W

I

V

G

G

M

R

S

N

A

V

I

S

I

I

L

M

K

K

G

G

V

V

T

S

V

V

G

G

E

S

G

G

A

S

I

V

V

I

A

G

A

Y

G

G

S

S

V

I

V

V

T

T

K

K

D

D

V

V

P

P

P

S

H

M

T

C

I

A

V

A

A

A

G

G

V

N

P

P

A

A

R

K

V

I

I

I

K

K

K

R

D

N

V

I

Y

I

Y

W

H210 (= H131) mutation to A: Loss of activity.
W234 (= W150) mutation to A: Loss of activity.

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
34% identity, 45% coverage: 105:194/201 of query aligns to 67:182/185 of 3nz2J

query
sites
3nz2J

L

F

H

H

C

C

F

E

-

F

-

G

N

K

H

T

I

I

E

R

I

I

G

G

E

D

N

H

V

T

I

F

I

I

S

N

E

M

N

N

V

V

I

V

I

M

R

L

D

D

S

G

-

A

-

P

-

I

-

T

-

I

D

G

N

D

H

H

Q

V

I

L

T

I

G
|
G

G

P

N

S

S

T

M

Q

F

F

-
x
Y

-

T

-

A

-

S

-
x
H

-

S

-

L

-

D

-

Y

-

R

-

Q

-

A

-

W

-

E

-

T

-

I

-

C

A

K

P

P

V

I

I

V

I

I

K

E

D

D

N

D

A

V

W
|
W

I

I

G
|
G

M

G

S

N

A

V

I

V

I

I

L

N

K

Q

G

G

V

V

T

T

V

I

G

G

E

A

G

R

A

S

I

V

V

V

A
|
A

G

N

S

S

V

V

T
x
N

K

Q

D

D

V

V

P

P

H

D

T

T

I

L

V

V

A
x
G

G

G

V
x
T

P
|
P

A

A

R

R

V

I

I

L

K
x
R

binding acetyl coenzyme *a: G104 (= G135), Y110 (vs. gap), H114 (vs. gap), W138 (= W150), G140 (= G152), A158 (= A170), A159 (= A171), N164 (≠ T176), G174 (≠ A186), T176 (≠ V188), P177 (= P189), R182 (≠ K194)

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
34% identity, 45% coverage: 105:194/201 of query aligns to 57:172/176 of 3ectA

query
sites
3ectA

L

F

H

H

C

C

F

E

-

F

-

G

N

K

H

T

I

I

E

R

I

I

G

G

E

D

N

H

V

T

I

F

I

I

S

N

E

M

N

N

V

V

I

V

I

M

R

L

D

D

S

G

-

A

-

P

-

I

-

T

-

I

D

G

N

D

H

H

Q

V

I

L

T

I

G

G

G

P

N

S

S

T

M

Q

F

F

-

Y

-

T

-

A

-

S

-

H

-

S

-

L

-

D

-

Y

-

R

-

Q

-

A

-

W

-

E

-

T

-

I

-

C

A

K

P

P

V

I

I

V

I

I

K

E

D

D

N

D

A

V

W

W

I

I

G

G

M

G

S

N

A

V

I

V

I

I

L

N

K

Q

G
|
G

V

V

T

T

V

I

G

G

E

A

G

R

A

S

I

V

V

V

A

A

G

N

S

S

V

V

T

N

K

Q

D
|
D

V

V

P

P

H

D

T

T

I

L

V

V

A

G

G

G

V

T

P

P

A

A

R

R

V

I

I

L

K

R

binding calcium ion: G138 (= G160), D156 (= D178)

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
34% identity, 45% coverage: 105:194/201 of query aligns to 64:179/183 of 3nz2C

query
sites
3nz2C

L

F

H

H

C

C

F

E

-

F

-

G

N

K

H

T

I

I

E

R

I

I

G

G

E

D

N

H

V

T

I

F

I

I

S

N

E

M

N

N

V

V

I

V

I

M

R

L

D

D

S

G

-

A

-

P

-

I

-

T

-

I

D

G

N

D

H

H

Q

V

I

L

T

I

G

G

G

P

N

S

S

T

M

Q

F

F

-
x
Y

-

T

-

A

-

S

-
x
H

-

S

-

L

-

D

-

Y

-

R

-

Q

-

A

-

W

-

E

-

T

-

I

-

C

A

K

P

P

V

I

I

V

I

I

K

E

D

D

N

D

A

V

W

W

I

I

G
|
G

M

G

S

N

A

V

I

V

I

I

L

N

K

Q

G

G

V

V

T

T

V

I

G

G

E

A

G

R

A

S

I

V

V

V

A
|
A

G
x
N

S

S

V

V

T
x
N

K

Q

D

D

V

V

P

P

H

D

T

T

I

L

V

V

A
x
G

G

G

V
x
T

P
|
P

A

A

R

R

V

I

I
x
L

K
x
R

binding acetyl coenzyme *a: Y107 (vs. gap), H111 (vs. gap), G137 (= G152), A155 (= A170), A156 (= A171), N161 (≠ T176), G171 (≠ A186), T173 (≠ V188), P174 (= P189), L178 (≠ I193), R179 (≠ K194)
binding magnesium ion: N157 (≠ G172), T173 (≠ V188)

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
36% identity, 49% coverage: 96:194/201 of query aligns to 82:184/188 of 3igjC

query
sites
3igjC

S

S

G

F

Y
x
F

I

A

N

N

F

F

H

N

S

C

K

V

L

I

H

L

C

D

F

V

N

C

H

E

I

V

E

R

I

I

G

G

E

D

N

H

V

C

I

M

I

F

S
x
A

E

P

N

G

V

V

I

H

I

I

R
x
Y

D

T

S
x
A

D

T

N
x
H

H

P

-

L

-

H

-

P

-

V

Q

E

I

R

T

N

G

S

G

G

N

K

S

E

M

Y

F

G

A

K

P

P

V

V

I

K

I

I

K

G

D

N

N

N

A

V

W

W

I

V

G
|
G

M

G

S

G

A

A

I

I

L
x
N

K

P

G

G

V

V

T

S

V

I

G

G

E

D

G

N

A

A

I

V

V

I

A
|
A

A
x
S

G

G

S

A

V

V

T
|
T

K

K

D

D

V

V

P

P

P

N

H

N

T

V

I

V

V

V

A

G

G

G

V
x
N

P

P

A

A

R

K

V

V

I

I

K

K

binding acetyl coenzyme *a: F84 (≠ Y98), A106 (≠ S120), Y112 (≠ R126), A114 (≠ S128), H116 (≠ N130), G142 (= G152), N148 (≠ L158), A160 (= A170), S161 (≠ A171), T166 (= T176), N178 (≠ V188)

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
44% identity, 42% coverage: 111:195/201 of query aligns to 158:242/258 of 8i04A

query
sites
8i04A

I

I

E

V

I

V

G

G

E

E

N

T

V

A

I

V

I

I

S

E

E

D

N

D

V

V

I

S

I

I

R

L

D

Q

S

S

D

V

N

T

H

L

Q

G

I
x
G

T

T

G

G

G

K

N

T

S

S

M

G

F

D

A
x
R

P
x
H

V

P

I

K

I

I

K

R

D

E

N

G

A

V

W

M

I

I

G

G

M

A

S

G

A

A

I

K

I

I

L

L

K

G

G

N

V

I

T

E

V

V

G

G

E

R

G

G

A

A

I

K

V

I

A

G

A

A

G

G

S

S

V

V

T

L

K

Q

D

P

V

V

P

P

H

H

T

T

I

T

V

A

A

A

G

G

V

V

P

P

A

A

R

R

V

I

I

V

K

G

K

K

binding serine: G180 (≠ I133), R188 (≠ A141), H189 (≠ P142)

Sites not aligning to the query:

binding serine: 88, 89, 153, 154

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
44% identity, 42% coverage: 111:195/201 of query aligns to 161:245/246 of 8i09A

query
sites
8i09A

I

I

E

V

I

V

G
|
G

E

E

N

T

V

A

I

V

I

I

S

E

E

D

N

D

V

V

I

S

I

I

R

L

D

Q

S

S

D

V

N

T

H

L

Q

G

I
x
G

T
|
T

G

G

G

K

N

T

S

S

M

G

F

D

A
x
R

P
x
H

V

P

I

K

I

I

K

R

D

E

N

G

A

V

W

M

I

I

G
|
G

M

A

S

G

A

A

I

K

I

I

L

L

K

G

G

N

V

I

T

E

V

V

G

G

E

R

G

G

A

A

I
x
K

V
x
I

A
x
G

A

A

G

G

S

S

V

V

T
x
L

K

Q

D

P

V

V

P

P

H

H

T

T

I

T

V

A

A
|
A

G

G

V

V

P

P

A

A

R

R

V

I

I

V

K

G

K

K

binding cysteine: G164 (= G114), R191 (≠ A141), H192 (≠ P142)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (≠ I133), T184 (= T134), G202 (= G152), K218 (≠ I168), I219 (≠ V169), G220 (≠ A170), L226 (≠ T176), A236 (= A186)

Sites not aligning to the query:

binding cysteine: 91, 156, 157

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
43% identity, 41% coverage: 111:193/201 of query aligns to 162:244/244 of 8i06A

query
sites
8i06A

I

I

E

V

I

V

G

G

E

E

N

T

V

A

I

V

I

I

S

E

E

D

N

D

V

V

I

S

I

I

R
x
L

D
x
Q

S

S

D

V

N

T

H

L

Q

G

I

G

T

T

G

G

G

K

N

T

S

S

M

G

F

D

A

R

P

H

V

P

I

K

I

I

K

R

D

E

N

G

A

V

W

M

I

I

G
|
G

M
x
A

S

G

A

A

I

K

I

I

L

L

K

G

G

N

V

I

T

E

V

V

G

G

E

R

G

G

A

A

I

K

V

I

A
x
G

A
|
A

G

G

S

S

V

V

T

L

K

Q

D

P

V

V

P

P

H

H

T

T

I
x
T

V

A

A
|
A

G

G

V

V

P

P

A

A

R

R

V

I

I

V

binding coenzyme a: L177 (≠ R126), Q178 (≠ D127), G203 (= G152), A204 (≠ M153), G221 (≠ A170), A222 (= A171), T235 (≠ I184), A237 (= A186)

Sites not aligning to the query:

7s45A Crystal structure of an n-acetyltransferase, c80t mutant, from helicobacter pullorum in the presence of acetyl coenzyme a and dtdp (see paper)
40% identity, 49% coverage: 84:182/201 of query aligns to 25:134/141 of 7s45A

query
sites
7s45A

V

C

V

V

L

L

P

P

D

S

A

A

K

-

L

-

I

I

L

I

G

G

S

E

G

-

Y

-

I

-

N

N

F

C

H

N

S

I

K

C

L

S

H

H

C

C

F
|
F

-

I

-
x
E

N

N

H

D

I

V

E

K

I

I

G

G

E

N

N

N

V

V

I

T

I

I

S

K

E

C

N

G

V

V

I

Q

I

I

R
x
W

D

D

S

G

-

I

-

E

-

I

D

E

N

D

H

D

Q

V

I

F

T

I

G

G

G

P

N

N

-

V

-

T

-

F

-
x
T

-
x
N

-
x
D

-
x
K

-
x
Y

-
x
P

-

R

-

S

S

K

M

Q

F

F

A

S

P

K

V

T

I

I

K

K

D

K

N

G

A

A

W

S

I

I

G

G

M

A

S

N

A

A

I

T

I

I

L
|
L

K

P

G

G

V

I

T

T

V

I

G

G

E

E

G

N

A

A

I

M

V

I

A

G

A

A

G

G

S

A

V

I

V

V

T
|
T

K
|
K

D

D

V

V

P

L

P

P

H

H

binding acetyl coenzyme *a: W64 (≠ R126), T82 (vs. gap), N83 (vs. gap), D84 (vs. gap), K85 (vs. gap), Y86 (vs. gap), P87 (vs. gap), L110 (= L158), T128 (= T176), K129 (= K177)
binding thymidine-5'-diphosphate: F44 (= F108), E46 (vs. gap)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
41% identity, 42% coverage: 111:195/201 of query aligns to 158:242/258 of 4h7oA

query
sites
4h7oA

I

I

E

V

I

I

G

G

E

E

N

T

V

A

I

V

S

E

E

D

N

D

V

V

I

S

I

I

R

L

D

Q

S

D

D

V

N

T

H

L

Q

G

I
x
G

T

T

G

G

G

K

N

E

S

C

M

G

F

D

A
x
R

P
x
H

V

P

I

K

I

I

K

R

D

E

N

G

A

V

W

M

I

I

G

G

M

A

S

G

A

A

I

K

I

I

L

L

K

G

G

N

V

I

T

E

V

V

G

G

E

E

G

G

A

A

I

K

V

I

A

G

A

S

G

G

S

S

V

V

T

L

K

Q

D

A

V

V

P

P

H

H

T

T

I

T

V

V

A

A

G

G

V

V

P

P

A

A

R

R

V

I

I

V

K

G

K

R

binding cysteine: G180 (≠ I133), R188 (≠ A141), H189 (≠ P142)

Sites not aligning to the query:

binding arginine: 51, 53, 127, 131
binding cysteine: 88, 89, 153, 154

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
42% identity, 42% coverage: 111:195/201 of query aligns to 162:246/262 of 1t3dA

query
sites
1t3dA

I

I

E

V

I

V

G

G

E

E

N

T

V

A

I

V

I

I

S

E

E

N

N

D

V

V

I

S

I

I

R

L

D

Q

S

S

D

V

N

T

H

L

Q

G

I
x
G

T

T

G

G

G

K

N

S

S

G

M

G

F

D

A
x
R

P
x
H

V

P

I

K

I

I

K

R

D

E

N

G

A

V

W

M

I

I

G

G

M

A

S

G

A

A

I

K

I

I

L

L

K

G

G

N

V

I

T

E

V

V

G

G

E

R

G

G

A

A

I

K

V

I

A

G

A

A

G

G

S

S

V

V

T

L

K

Q

D

P

V

V

P

P

H

H

T

T

I

T

V

A

A

A

G

G

V

V

P

P

A

A

R

R

V

I

I

V

K

G

K

K

binding cysteine: G184 (≠ I133), R192 (≠ A141), H193 (≠ P142)

Sites not aligning to the query:

binding cysteine: 92, 93, 157

Q5KW03 Carbonic anhydrase; Gamma-carbonic anhydrase; Cag; EC 4.2.1.1 from Geobacillus kaustophilus (strain HTA426) (see paper)
37% identity, 41% coverage: 113:195/201 of query aligns to 51:142/182 of Q5KW03

query
sites
Q5KW03

I

I

G

G

E

N

N

R

V

V

I
x
N

I

I

S
x
Q

E

D

N

N

V

S

I

I

I

L

R
x
H

D

Q

S

S

D

P

N

N

H

N

Q

P

I

L

-

I

-

E

-

D

-

G

-

V

-

T

T

V

G

G

G
x
H

N

Q

S

V

M

I

F

L

A
x
H

P

S

V

A

I

I

I

V

K

R

D

K

N

N

A

A

W

L

I

I

G

G

M

M

S

G

A

S

I

I

L

L

K

D

G

R

V

A

T

E

V

I

G

G

E

E

G

G

A

A

I

F

V

I

A

G

A

A

G

G

S

S

V

L

V

V

T

P

-

P

-

G

K

K

D

K

V

I

P

P

H

N

T

T

I

L

V

A

A

L

G

G

V

R

P

P

A

A

R

K

V

V

I

V

K

R

K

E

N56 (≠ I118) mutation to A: Retains 65% of wild-type activity.
Q58 (≠ S120) mutation to A: Retains 20% of wild-type activity.
H64 (≠ R126) binding ; mutation to A: Retains 20% of wild-type activity.
H81 (≠ G136) binding
H86 (≠ A141) binding

Sites not aligning to the query:

43 R→A: Retains 45% of wild-type activity.
158 Y→A: Retains 10% of wild-type activity.

3vnpA Crystal structure of hypothetical protein (gk2848) from geobacillus kaustophilus
37% identity, 41% coverage: 113:195/201 of query aligns to 52:143/171 of 3vnpA

query
sites
3vnpA

I

I

G

G

E

N

N

R

V

V

I

N

I

I

S

Q

E

D

N

N

V

S

I

I

I

L

R
x
H

D

Q

S

S

D

P

N

N

H

N

Q

P

I

L

-

I

-

E

-

D

-

G

-

V

-

T

T

V

G

G

G

H

N

Q

S

V

M

I

F

L

A
x
H

P

S

V

A

I

I

I

V

K

R

D

K

N

N

A

A

W

L

I

I

G

G

M

M

S

G

A

S

I

I

L

L

K

D

G

R

V

A

T

E

V

I

G

G

E

E

G

G

A

A

I

F

V

I

A

G

A

A

G

G

S

S

V

L

V

V

T

P

-

P

-

G

K

K

D

K

V

I

P

P

H

N

T

T

I

L

V

A

A

L

G

G

V

R

P

P

A

A

R

K

V

V

I

V

K

R

K

E

binding magnesium ion: H65 (≠ R126), H87 (≠ A141)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
42% identity, 42% coverage: 111:195/201 of query aligns to 165:249/272 of 3gvdI

query
sites
3gvdI

I

I

E

V

I

I

G

G

E

E

N

T

V

A

I

V

S

E

E

N

N

D

V

V

I

S

I

I

R

L

D

Q

S

S

D

V

N

T

H

L

Q
x
G

I
x
G

T

T

G

G

G

K

N

T

S

S

M

G

F

D

A
x
R

P

H

V

P

I

K

I

I

K

R

D

E

N

G

A

V

W

M

I

I

G

G

M

A

S

G

A

A

I

K

I

I

L

L

K

G

G

N

V

I

T

E

V

V

G

G

E

R

G

G

A

A

I

K

V

I

A

G

A

A

G

G

S

S

V

V

T

L

K

Q

D

S

V

V

P

P

P

A

H

H

T

T

I

T

V

A

A

A

G

G

V

V

P

P

A

A

R

R

V

I

I

V

K

G

K

K

binding cysteine: G186 (≠ Q132), G187 (≠ I133), R195 (≠ A141)

Sites not aligning to the query:

binding cysteine: 95, 96, 160

Query Sequence

>352404 FitnessBrowser__Btheta:352404
MIFGFIAKIYRKYQEYIHPGIYVTRHGILYREKDCRYNVYPLQRLIVGKVWVGNIPSQEK
GRLILHANSELIVKGNFDIIGSTVVVLPDAKLILGSGYINFHSKLHCFNHIEIGENVIIS
ENVIIRDSDNHQITGGNSMFAPVIIKDNAWIGMSAIILKGVTVGEGAIVAAGSVVTKDVP
PHTIVAGVPARVIKKDVYYTI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory