SitesBLAST

P0A796 ATP-dependent 6-phosphofructokinase isozyme 1; ATP-PFK 1; Phosphofructokinase 1; 6-phosphofructokinase isozyme I; Phosphohexokinase 1; Sedoheptulose-7-phosphate kinase; EC 2.7.1.11 from Escherichia coli (strain K12) (see 2 papers)
41% identity, 90% coverage: 2:303/336 of query aligns to 4:292/320 of P0A796

query
sites
P0A796

R

K

I

I

G

G

I

V

L

L

T

T

S

S

G

G

G
|
G

D

D

C

A

P

P

G

G

I

M

N

N

A

A

T

A

I

I

R
|
R

G
|
G

V
|
V

C
x
V

K
x
R

T

S

A

A

I

L

N

T

H

E

Y

-

G

G

M

L

E

E

V

V

I

M

G

G

I

I

H

Y

S

D

G

G

F

Y

Q

L

G

G

L

L

L

Y

T

E

K

D

D

R

I

M

E

V

S

Q

F

L

T

D

D
x
R

K
x
Y

S
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S

L
x
V

S
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S

G
x
D

L

M

L

I

N

N

Q

R

G

G

T

T

M

F

L

L

G

G

T

S

S

A

R
|
R

E
x
F

K

P

P

E

F

F

K

R

K

-

G

-

V

-

S

-

D

D

V

E

D

N

K

I

P

R

A

A

L

V

I

A

L

I

Q

E

N

N

I

L

Q

K

E

K

M

R

G

G

L

I

D

D

C

A

V

L

V

V

C

V

I

I

G

G

G
|
G

N
x
D

G
|
G

T
x
S

Q

Y

K

M

T

G

A

A

A

M

K

R

F

L

A

T

A

E

M

M

G

G

V

F

N

P

I

C

V

I

S

G

V

L

P

P

K

G

T
|
T

I
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I

D
|
D

N

N

D

D

I

I

W

K

G

G

T

T

D

D

I

Y

S

T

F

I

G

G

F

F

D

F

S

T

A

A

V

L

S

S

I

T

A

V

T

V

D

E

A

A

I

I

D

D

R

R

L

L

H
x
R

S

D

T

T

A

S

S

S

H

H

K

Q

R
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R

V

I

M

S

V

V

I

V

E

E

V

V

M

M

G

G

H
x
R

K

Y

A

C

G

G

W

D

I

L

A

T

L

L

Y

A

S

A

G

A

M

I

A

A

G

G

G
|
G

G
x
C

D
x
E

V

F

I

V

L

V

V

V

P

P

E

E

I

V

P

E

Y

F

N

S

I

R

K

E

N

D

I

L

G

V

N

N

T

E

I

I

L

K

E

A

R

G

L

I

K

A

K

K

G

G

K
|
K

P
x
K

Y
x
H

S

A

I

I

V

V

V

A

V

I

A

T

E
|
E

G

H

I

M

L

C

T

D

D

-

G

-

R

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K

-

R

-

A

-

A

V

E

D

Y

E

I

L

A

A

Q

H

E

F

I

I

E

E

Y

K

E

E

T

T

G

G

I

R

E

E

T

T

R
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R

E

A

T

T

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V

L

L

G

G

Y
x
H

I
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I

Q
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Q

R
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R

G

G

S

S

P

P

T

V

P

P

F

Y

D

D

R

R

N

I

L

L

S

A

T

S

R

R

M

M

G

G

G

A

H

Y

A

A

T

I

E

D

L

L

I

L

A

L

N

A

G

G

Q

Y

F

G

G

G

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R

M

C

I

V

A

G

L

I

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Q

G

N

D

E

D

Q

I

L

G12 (= G10) binding
RGVVR 22:26 (≠ RGVCK 20:24) binding
RYSVSD 55:60 (≠ DKSLSG 54:59) binding
RF 73:74 (≠ RE 72:73) binding
GDGS 103:106 (≠ GNGT 108:111) binding
D104 (≠ N109) binding
TID 126:128 (= TID 131:133) binding in other chain
D128 (= D133) active site, Proton acceptor; mutation to S: 18000-fold reduction of catalytic rate.
R155 (≠ H160) binding in other chain
R163 (= R168) binding
R172 (≠ H177) mutation to S: 3.4-fold reduction in turnover numbers.
GCE 186:188 (≠ GGD 191:193) binding in other chain
KKH 214:216 (≠ KPY 219:221) binding in other chain
E223 (= E228) binding in other chain
R244 (= R255) binding
HIQR 250:253 (≠ YIQR 261:264) binding in other chain

6pfkA Phosphofructokinase, inhibited t-state (see paper)
42% identity, 92% coverage: 2:310/336 of query aligns to 3:298/319 of 6pfkA

query
sites
6pfkA

R

R

I

I

G

G

I

V

L

L

T

T

S

S

G

G

G
|
G

D

D

C

S

P

P

G

G

I

M

N

N

A

A

T

A

I

I

R
|
R

G

S

V

V

C

V

K
x
R

T

K

A

A

I

I

N

Y

H

H

Y

-

G

G

M

V

E

E

V

V

I

Y

G

G

I

V

H

Y

S

H

G

G

F

Y

Q

A

G

G

L

L

L

I

T

A

K

G

D

N

I

I

E

K

S

K

F

L

T

E

D

V

K

G

S

D

L

V

S
x
G

G
x
D

L

I

N

H

Q

R

G

G

T

T

M

I

L

L

G

Y

T

T

S

A

R
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R

E
x
C

K

P

P

E

F

F

K

K

K

-

G

-

V

-

V

T

S

E

D

E

V

G

D

Q

K

K

P

K

A

G

L

I

I

-

L

-

Q

E

N

Q

I

L

Q

K

E

K

M

H

G

G

L

I

D

E

C

G

V

L

V

V

C

V

I

I

G

G

N
x
D

G
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G

T

S

Q

Y

K

Q

T

G

A

A

A

K

K

K

F

L

A

T

A

E

M

H

G

G

V

F

N

P

I

C

V

V

S

G

V

V

P

P

K
x
G

T
|
T

I

I

D
|
D

N

N

D
|
D

I

I

W

P

G

G

T

T

D

D

I

F

S

T

F

I

G

G

F

F

D

D

S

T

A

A

V

L

S

N

I

T

A

V

T

I

D

D

A

A

I

I

D

D

R

K

L

I

H
x
R

S

D

T

T

A

A

S

T

S

S

H

H

K

E

R

R

V

T

M

Y

V

V

I

I

E

E

V

V

M

M

G

G

H
x
R

K

H

A

A

G

G

W

D

I

I

A

A

L

L

Y

W

S

S

G

G

M

L

A

A

G

G

G

A

D

E

V

T

I

I

L

L

V

I

P

P

E

E

I

A

P

D

Y

Y

N

D

I

M

K

-

N

-

I

-

G

-

N

N

T

D

I

V

L

I

E

A

R

R

L

L

K

K

-

R

-

G

-

H

-

E

K
x
R

G

G

K
|
K

P

K

Y

H

S

S

I

I

V

I

V

V

A

A

E

E

G

G

I

V

L

-

T

-

D

-

G

-

R

-

K

-

R

G

A

S

A

G

E

V

Y

D

I

F

A

G

Q

R

E

Q

I

I

E

Q

Y

E

E

A

T

T

G

G

I

F

E

E

T

T

R

R

E

V

T

T

V

V

L

L

G

G

Y

H

I

V

Q

Q

R

R

G

G

S

S

P

P

T

T

P

A

F

F

D

D

R

R

N

V

L

L

S

A

T

S

R

R

M

L

G

G

G

A

H

R

A

A

T

V

E

E

L

L

I

L

A

L

N

E

G

G

Q

K

F

G

G

G

R

R

M

C

I

V

A

G

L

I

K

Q

G

N

D

N

D

Q

I

L

S

V

S

D

I

H

P

D

L

I

E

A

E

E

active site: G11 (= G10), R72 (= R72), C73 (≠ E73), D103 (≠ N109), G104 (= G110), G124 (≠ K130), T125 (= T131), D127 (= D133), D129 (= D135), R171 (≠ H177)
binding 2-phosphoglycolic acid: R21 (= R20), R25 (≠ K24), G58 (≠ S58), D59 (≠ G59), R154 (≠ H160), R211 (≠ K217), K213 (= K219)

P00512 ATP-dependent 6-phosphofructokinase; ATP-PFK; Phosphofructokinase; Phosphohexokinase; EC 2.7.1.11 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see 2 papers)
42% identity, 92% coverage: 2:310/336 of query aligns to 3:298/319 of P00512

query
sites
P00512

R

R

I

I

G

G

I

V

L

L

T

T

S

S

G

G

D

D

C

S

P

P

G

G

I

M

N

N

A

A

T

A

I

I

R
|
R

G
x
S

V
|
V

C
x
V

K
x
R

T

K

A

A

I

I

N

Y

H

H

Y

-

G

G

M

V

E

E

V

V

I

Y

G

G

I

V

H

Y

S

H

G

G

F

Y

Q

A

G

G

L

L

L

I

T

A

K

G

D

N

I

I

E

K

S

K

F

L

T

E

D

V

K

G

S

D

L

V

S

G

G
x
D

L

I

N

H

Q

R

G

G

T

T

M

I

L

L

G

Y

T

T

S

A

R
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R

E
x
C

K

P

P

E

F

F

K

K

K

-

G

-

V

-

V

T

S

E

D

E

V

G

D

Q

K

K

P

K

A

G

L

I

I

-

L

-

Q

E

N

Q

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E

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M

H

G

G

L

I

D

E

C

G

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L

V

V

C

V

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I

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G

N
x
D

G

G

T

S

Q

Y

K

Q

T

G

A

A

A

K

K

K

F

L

A

T

A

E

M

H

G

G

V

F

N

P

I

C

V

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S

G

V

V

P

P

K

G

T
|
T

I
|
I

D
|
D

N

N

D

D

I

I

W

P

G

G

T

T

D

D

I

F

S

T

F

I

G

G

F

F

D

D

S

T

A

A

V

L

S

N

I

T

A

V

T

I

D

D

A

A

I

I

D

D

R

K

L

I

H
x
R

S

D

T

T

A

A

S

T

S

S

H

H

K

E

R
|
R

V

T

M

Y

V

V

I

I

E

E

V

V

M
|
M

G
|
G

H
x
R

K

H

A

A

G

G

W

D

I

I

A

A

L

L

Y

W

S

S

G

G

M

L

A

A

G

G

G
|
G

G
x
A

D
x
E

V

T

I

I

L

L

V

I

P

P

E

E

I

A

P

D

Y

Y

N

D

I

M

K

-

N

-

I

-

G

-

N

N

T

D

I

V

L

I

E

A

R

R

L

L

K

K

-

R

-

G

-

H

-

E

K
x
R

G

G

K
|
K

P
x
K

Y
x
H

S

S

I

I

V

I

V

V

A

A

E
|
E

G

G

I

V

L

-

T

-

D

-

G

-

R

-

K

-

R

G

A

S

A

G

E

V

Y

D

I

F

A

G

Q

R

E

Q

I

I

E

Q

Y

E

E

A

T

T

G

G

I

F

E

E

T

T

R
|
R

E

V

T

T

V

V

L

L

G

G

Y
x
H

I
x
V

Q
|
Q

R
|
R

G

G

S

S

P

P

T

T

P

A

F

F

D

D

R

R

N

V

L

L

S

A

T

S

R

R

M

L

G

G

G

A

H

R

A

A

T

V

E

E

L

L

I

L

A

L

N

E

G

G

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F

G

G

G

R

R

M

C

I

V

A

G

L

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K

Q

G

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N

D

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S

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S

D

I

H

P

D

L

I

E

A

E

E

RSVVR 21:25 (≠ RGVCK 20:24) binding
D59 (≠ G59) binding
RC 72:73 (≠ RE 72:73) binding
D103 (≠ N109) binding
TID 125:127 (= TID 131:133) binding in other chain
R154 (≠ H160) binding in other chain
R162 (= R168) binding
MGR 169:171 (≠ MGH 175:177) binding in other chain
GAE 185:187 (≠ GGD 191:193) binding in other chain
R211 (≠ K217) binding in other chain
KKH 213:215 (≠ KPY 219:221) binding in other chain
E222 (= E228) binding in other chain
R243 (= R255) binding
HVQR 249:252 (≠ YIQR 261:264) binding in other chain

4pfkA Phosphofructokinase. Structure and control (see paper)
42% identity, 92% coverage: 2:310/336 of query aligns to 3:298/319 of 4pfkA

query
sites
4pfkA

R

R

I

I

G

G

I

V

L

L

T

T

S
|
S

G

G

G
|
G

D

D

C

S

P

P

G

G

I

M

N

N

A

A

T

A

I

I

R

R

G

S

V

V

C

V

K

R

T

K

A

A

I

I

N

Y

H

H

Y

-

G

G

M

V

E

E

V

V

I

Y

G

G

I

V

H

Y

S

H

G

G

F
x
Y

Q

A

G

G

L

L

L

I

T

A

K

G

D

N

I

I

E

K

S

K

F

L

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E

D

V

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S

D

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S

G

G

D

L

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G

Y

T

T

S

A

R
|
R

E
x
C

K

P

P

E

F
|
F

K
|
K

K

-

G

-

V

-

V

T

S

E

D

E

V

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D

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K

K

P

K

A

G

L

I

I

-

L

-

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E

N

Q

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K

E

K

M

H

G

G

L

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D

Q

C

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V

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C

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I

I

G

G

N
x
D

G
|
G

T

S

Q

Y

K

Q

T
x
G

A

A

A

K

K

K

F

L

A

T

A

E

M

H

G

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I

C

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V

S

G

V

V

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P

K
x
G

T
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T

I

I

D
|
D

N

N

D
|
D

I

I

W

P

G

G

T

T

D

D

I

F

S

T

F

I

G

G

F

F

D

D

S

T

A

A

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N

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T

A

V

T

I

D

D

A

A

I

I

D

D

R

K

L

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H
x
R

S

D

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T

A

A

S

T

S

S

H

H

K

E

R

R

V

T

M

Y

V

V

I

I

E

E

V

V

M
|
M

G

G

H
x
R

K

H

A

A

G

G

W

D

I

I

A

A

L

L

Y

W

S

S

G

G

M

L

A

A

G

G

G
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G

G

A

D
x
E

V

T

I

I

L

L

V

I

P

P

E

E

I

A

P

D

Y

Y

N

D

I

M

K

-

N

-

I

-

G

-

N

N

T

D

I

V

L

I

E

A

R

R

L

L

K

K

-

R

-

G

-

H

-

E

K
x
R

G
|
G

K
|
K

P

K

Y
x
H

S

S

I

I

V

I

V

V

A

A

E
|
E

G

G

I

V

L

-

T

-

D

-

G

-

R

-

K

-

R

G

A

S

A

G

E

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Y

D

I

F

A

G

Q

R

E

Q

I

I

E

Q

Y

E

E

A

T

T

G

G

I

F

E

E

T

T

R

R

E

V

T

T

V

V

L

L

G

G

Y
x
H

I

V

Q

Q

R
|
R

G

G

S

S

P

P

T

T

P

A

F

F

D

D

R

R

N

V

L

L

S

A

T

S

R

R

M

L

G

G

G

A

H

R

A

A

T

V

E

E

L

L

I

L

A

L

N

E

G

G

Q

K

F

G

G

G

R

R

M

C

I

V

A

G

L

I

K

Q

G

N

D

N

D

Q

I

L

S

V

S

D

I

H

P

D

L

I

E

A

E

E

active site: G11 (= G10), R72 (= R72), C73 (≠ E73), D103 (≠ N109), G104 (= G110), G124 (≠ K130), T125 (= T131), D127 (= D133), D129 (= D135), R171 (≠ H177)
binding adenosine-5'-diphosphate: S9 (= S8), Y41 (≠ F41), R72 (= R72), C73 (≠ E73), F76 (= F76), K77 (= K77), G104 (= G110), G108 (≠ T114), R154 (≠ H160), G185 (= G191), R211 (≠ K217), G212 (= G218), K213 (= K219), H215 (≠ Y221)
binding 6-O-phosphono-beta-D-fructofuranose: D127 (= D133), M169 (= M175), E222 (= E228), H249 (≠ Y261), R252 (= R264)
binding magnesium ion: G185 (= G191), E187 (≠ D193)

3pfkA Phosphofructokinase. Structure and control (see paper)
42% identity, 92% coverage: 2:310/336 of query aligns to 3:298/319 of 3pfkA

query
sites
3pfkA

R

R

I

I

G

G

I

V

L

L

T

T

S

S

G

G

G
|
G

D

D

C

S

P

P

G

G

I

M

N

N

A

A

T

A

I

I

R

R

G

S

V

V

C

V

K

R

T

K

A

A

I

I

N

Y

H

H

Y

-

G

G

M

V

E

E

V

V

I

Y

G

G

I

V

H

Y

S

H

G

G

F

Y

Q

A

G

G

L

L

L

I

T

A

K

G

D

N

I

I

E

K

S

K

F

L

T

E

D

V

K

G

S

D

L

V

S

G

G

D

L

I

N

H

Q

R

G

G

T

T

M

I

L

L

G

Y

T

T

S

A

R
|
R

E
x
C

K

P

P

E

F

F

K

K

K

-

G

-

V

-

V

T

S

E

D

E

V

G

D

Q

K

K

P

K

A

G

L

I

I

-

L

-

Q

E

N

Q

I

L

Q

K

E

K

M

H

G

G

L

I

D

Q

C

G

V

L

V

V

C

V

I

I

G

G

N
x
D

G
|
G

T

S

Q

Y

K

Q

T

G

A

A

A

K

K

K

F

L

A

T

A

E

M

H

G

G

V

F

N

P

I

C

V

V

S

G

V

V

P

P

K
x
G

T
|
T

I

I

D
|
D

N

N

D
|
D

I

I

W

P

G

G

T

T

D

D

I

F

S

T

F

I

G

G

F

F

D

D

S

T

A

A

V

L

S

N

I

T

A

V

T

I

D

D

A

A

I

I

D

D

R

K

L

I

H
x
R

S

D

T

T

A

A

S

T

S

S

H

H

K

E

R

R

V

T

M

Y

V

V

I

I

E

E

V

V

M

M

G

G

H
x
R

K

H

A

A

G

G

W

D

I

I

A

A

L

L

Y

W

S

S

G

G

M

L

A

A

G

G

G

A

D

E

V

T

I

I

L

L

V

I

P

P

E

E

I

A

P

D

Y

Y

N

D

I

M

K

-

N

-

I

-

G

-

N

N

T

D

I

V

L

I

E

A

R

R

L

L

K

K

-

R

-

G

-

H

-

E

K

R

G

G

K
|
K

P

K

Y

H

S

S

I

I

V

I

V

V

A

A

E

E

G

G

I

V

L

-

T

-

D

-

G

-

R

-

K

-

R

G

A

S

A

G

E

V

Y

D

I

F

A

G

Q

R

E

Q

I

I

E

Q

Y

E

E

A

T

T

G

G

I

F

E

E

T

T

R

R

E

V

T

T

V

V

L

L

G

G

Y
x
H

I

V

Q

Q

R
|
R

G

G

S

S

P

P

T

T

P

A

F

F

D

D

R

R

N

V

L

L

S

A

T

S

R

R

M

L

G

G

G

A

H

R

A

A

T

V

E

E

L

L

I

L

A

L

N

E

G

G

Q

K

F

G

G

G

R

R

M

C

I

V

A

G

L

I

K

Q

G

N

D

N

D

Q

I

L

S

V

S

D

I

H

P

D

L

I

E

A

E

E

active site: G11 (= G10), R72 (= R72), C73 (≠ E73), D103 (≠ N109), G104 (= G110), G124 (≠ K130), T125 (= T131), D127 (= D133), D129 (= D135), R171 (≠ H177)
binding phosphate ion: R154 (≠ H160), K213 (= K219), H249 (≠ Y261), R252 (= R264)

4i4iA Crystal structure of bacillus stearothermophilus phosphofructokinase mutant t156a bound to pep (see paper)
42% identity, 92% coverage: 2:310/336 of query aligns to 3:298/319 of 4i4iA

query
sites
4i4iA

R

R

I

I

G

G

I

V

L

L

T

T

S

S

G

G

G
|
G

D

D

C

S

P

P

G

G

I

M

N

N

A

A

T

A

I

I

R
|
R

G

S

V

V

C

V

K
x
R

T

K

A

A

I

I

N

Y

H

H

Y

-

G

G

M

V

E

E

V

V

I

Y

G

G

I

V

H

Y

S

H

G

G

F

Y

Q

A

G

G

L

L

L

I

T

A

K

G

D

N

I

I

E

K

S

K

F

L

T

E

D

V

K

G

S

D

L

V

S
x
G

G

D

L

I

N

H

Q

R

G

G

T

T

M

I

L

L

G

Y

T

T

S

A

R
|
R

E
x
C

K

P

P

E

F

F

K

K

K

-

G

-

V

-

V

T

S

E

D

E

V

G

D

Q

K

K

P

K

A

G

L

I

I

-

L

-

Q

E

N

Q

I

L

Q

K

E

K

M

H

G

G

L

I

D

E

C

G

V

L

V

V

C

V

I

I

G

G

N
x
D

G
|
G

T

S

Q

Y

K

Q

T

G

A

A

A

K

K

K

F

L

A

T

A

E

M

H

G

G

V

F

N

P

I

C

V

V

S

G

V

V

P

P

K
x
G

T
|
T

I

I

D
|
D

N

N

D
|
D

I

I

W

P

G

G

T

T

D

D

I

F

S

T

F

I

G

G

F

F

D

D

S

T

A

A

V

L

S

N

I

T

A

V

T

I

D

D

A

A

I

I

D

D

R

K

L

I

H
x
R

S

D

T

A

A

A

S

T

S

S

H

H

K

E

R

R

V

T

M

Y

V

V

I

I

E

E

V

V

M

M

G

G

H
x
R

K

H

A

A

G

G

W

D

I

I

A

A

L

L

Y

W

S

S

G

G

M

L

A

A

G

G

G

A

D

E

V

T

I

I

L

L

V

I

P

P

E

E

I

A

P

D

Y

Y

N

D

I

M

K

-

N

-

I

-

G

-

N

N

T

D

I

V

L

I

E

A

R

R

L

L

K

K

-

R

-

G

-

H

-

E

K
x
R

G

G

K
|
K

P

K

Y
x
H

S

S

I

I

V

I

V

V

A

A

E

E

G

G

I

V

L

-

T

-

D

-

G

-

R

-

K

-

R

G

A

S

A

G

E

V

Y

D

I

F

A

G

Q

R

E

Q

I

I

E

Q

Y

E

E

A

T

T

G

G

I

F

E

E

T

T

R

R

E

V

T

T

V

V

L

L

G

G

Y

H

I

V

Q

Q

R

R

G

G

S

S

P

P

T

T

P

A

F

F

D

D

R

R

N

V

L

L

S

A

T

S

R

R

M

L

G

G

G

A

H

R

A

A

T

V

E

E

L

L

I

L

A

L

N

E

G

G

Q

K

F

G

G

G

R

R

M

C

I

V

A

G

L

I

K

Q

G

N

D

N

D

Q

I

L

S

V

S

D

I

H

P

D

L

I

E

A

E

E

active site: G11 (= G10), R72 (= R72), C73 (≠ E73), D103 (≠ N109), G104 (= G110), G124 (≠ K130), T125 (= T131), D127 (= D133), D129 (= D135), R171 (≠ H177)
binding phosphoenolpyruvate: R21 (= R20), R25 (≠ K24), G58 (≠ S58), R154 (≠ H160), R211 (≠ K217), K213 (= K219), H215 (≠ Y221)

5xz7A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
38% identity, 93% coverage: 2:315/336 of query aligns to 3:305/322 of 5xz7A

query
sites
5xz7A

R

K

I

I

G

A

I

V

L

L

T

T

S

S

G

G

D

D

C

S

P

P

G

G

I

M

N

N

A

A

T

A

I

V

R

R

G

A

V

V

C

V

K

R

T

T

A

A

I

I

N

-

H

Y

Y

N

G

E

M

I

E

E

V

V

I

Y

G

G

I

V

H

Y

S

H

G

G

F

Y

Q

Q

G

G

L

L

T

N

K

D

D

D

I

I

E

H

S

K

F

L

T

E

D

L

K

G

S

S

L

V

S

G

G

D

L

T

L

I

N

Q

Q

R

G

G

T

T

M

F

L

L

G

Y

T

S

S

A

R
|
R

E

C

K

P

P

E

F

F

K

K

K

E

G

-

V

-

S

Q

D

E

V

V

D

R

K

K

P

V

A

A

L

I

I

-

L

-

Q

E

N

N

I

L

Q

R

E

K

M

R

G

G

L

I

D

E

C

G

V

L

V

V

C

V

I

I

G

G

N

D

G

G

T

S

Q

Y

K

R

T

G

A

A

A

Q

K

R

F

I

A

S

-

E

A

C

M

K

G

E

V

I

N

Q

I

T

V

I

S

G

V

I

P

P

K

G

T

T

I

I

D
|
D

N

N

D

D

I

I

W

N

G

G

T

T

D

D

I

F

S

T

F

I

G

G

F

F

D

D

S

T

A

A

V

L

S

N

I

T

A

I

T

I

D

G

A

L

I

V

D

D

R

K

L

I

H

R

S

D

T

T

A

A

S

S

H

H

K

A

R

R

V

T

M

F

V

I

I

I

E

E

V

A

M
|
M

G

G

H
x
R

K

D

A

C

G

G

W

D

I

L

A

A

L

L

Y

W

S

A

G

G

M

L

A

S

G

V

G

G

G

A

D

E

V

T

I

I

L

V

V

V

P

P

E

E

I

V

P

K

Y

T

N

D

I

I

K

K

N

E

I

I

G

A

N

D

T

K

I

I

L

E

E

Q

R

G

L

I

K

K

K

R

G

G

K

K

P

K

Y

H

S

S

I

I

V

V

V

L

V

V

A

A

E
|
E

G

G

I

C

L

M

T

T

-

A

-

Q

D

D

G

C

R

Q

K

K

R

E

A

L

A

S

E

Q

Y

Y

I

I

A

-

Q

-

E

-

I

-

E

-

Y

-

E

-

T

-

G

N

I

V

E

D

T

N

R

R

E

V

T

S

V

V

L

L

G

G

Y
x
H

I

V

Q

Q

R
|
R

G

G

S

S

P

P

T

T

P

G

F

A

D

D

R

R

N

V

L

L

S

A

T

S

R

R

M

L

G

G

H

Y

A

A

T

V

E

D

L

L

I

L

A

M

N

Q

G

G

Q

E

F

T

G

A

R

K

M

G

I

V

A

G

L

I

K

K

G

N

D

N

D

K

I

I

S

V

S

A

I

T

P

S

L

F

E

D

E

E

V

I

A

F

G

D

K

K

L

F

binding 6-O-phosphono-beta-D-fructofuranose: R72 (= R72), D129 (= D133), M171 (= M175), R173 (≠ H177), E224 (= E228), H251 (≠ Y261), R254 (= R264)

5xz6A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
38% identity, 93% coverage: 2:315/336 of query aligns to 3:305/318 of 5xz6A

query
sites
5xz6A

R

K

I

I

G

A

I

V

L

L

T

T

S

S

G

G

D

D

C

S

P

P

G

G

I

M

N

N

A

A

T

A

I

V

R

R

G

A

V

V

C

V

K

R

T

T

A

A

I

I

N

-

H

Y

Y

N

G

E

M

I

E

E

V

V

I

Y

G

G

I

V

H

Y

S

H

G

G

F
x
Y

Q

Q

G

G

L

L

T

N

K

D

D

D

I

I

E

H

S

K

F

L

T

E

D

L

K

G

S

S

L

V

S

G

G

D

L

T

L

I

N

Q

Q

R

G

G

T

T

M

F

L

L

G

Y

T

S

S

A

R
|
R

E
x
C

K

P

P

E

F

F

K
|
K

K

E

G

-

V

-

S

Q

D

E

V

V

D

R

K

K

P

V

A

A

L

I

I

-

L

-

Q

E

N

N

I

L

Q

R

E

K

M

R

G

G

L

I

D

E

C

G

V

L

V

V

C

V

I

I

G

G

G
|
G

N

D

G
|
G

T
x
S

Q

Y

K
x
R

T

G

A

A

A

Q

K

R

F

I

A

S

-

E

A

C

M

K

G

E

V

I

N

Q

I

T

V

I

S

G

V

I

P

P

K

G

T

T

I

I

D

D

N

N

D

D

I

I

W

N

G

G

T

T

D

D

I

F

S

T

F

I

G

G

F

F

D

D

S

T

A

A

V

L

S

N

I

T

A

I

T

I

D

G

A

L

I

V

D

D

R

K

L

I

H

R

S

D

T

T

A

A

S

S

H

H

K

A

R

R

V

T

M

F

V

I

I

I

E

E

V

A

M

M

G

G

H
x
R

K

D

A

C

G

G

W

D

I

L

A

A

L

L

Y

W

S

A

G

G

M

L

A

S

G

V

G

G

G

A

D

E

V

T

I

I

L

V

V

V

P

P

E

E

I

V

P

K

Y

T

N

D

I

I

K

K

N

E

I

I

G

A

N

D

T

K

I

I

L

E

E

Q

R

G

L

I

K

K

K

R

G

G

K

K

P

K

Y

H

S

S

I

I

V

V

V

L

V

V

A

A

E

E

G

G

I

C

L

M

T

T

-

A

-

Q

D

D

G

C

R

Q

K

K

R

E

A

L

A

S

E

Q

Y

Y

I

I

A

-

Q

-

E

-

I

-

E

-

Y

-

E

-

T

-

G

N

I

V

E

D

T

N

R

R

E

V

T

S

V

V

L

L

G

G

Y

H

I

V

Q

Q

R

R

G

G

S

S

P

P

T

T

P

G

F

A

D

D

R

R

N

V

L

L

S

A

T

S

R

R

M

L

G

G

H

Y

A

A

T

V

E

D

L

L

I

L

A

M

N

Q

G

G

Q

E

F

T

G

A

R

K

M

G

I

V

A

G

L

I

K

K

G

N

D

N

D

K

I

I

S

V

S

A

I

T

P

S

L

F

E

D

E

E

V

I

A

F

G

D

K

K

L

F

binding phosphoaminophosphonic acid-adenylate ester: Y41 (≠ F41), R72 (= R72), C73 (≠ E73), K77 (= K77), G102 (= G108), G104 (= G110), S105 (≠ T111), R107 (≠ K113), R173 (≠ H177)

5xzaA Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adp (see paper)
39% identity, 92% coverage: 2:311/336 of query aligns to 3:301/322 of 5xzaA

query
sites
5xzaA

R

K

I

I

G

A

I

V

L

L

T

T

S
|
S

G

G

D

D

C

S

P

P

G

G

I

M

N

N

A

A

T

A

I

V

R

R

G

A

V

V

C

V

K

R

T

T

A

A

I

I

N

-

H

Y

Y

N

G

E

M

I

E

E

V

V

I

Y

G

G

I

V

H

Y

S

H

G

G

F
x
Y

Q

Q

G

G

L

L

T

N

K

D

D

D

I

I

E

H

S

K

F

L

T

E

D

L

K

G

S

S

L

V

S

G

G

D

L

T

L

I

N

Q

Q

R

G

G

T

T

M

F

L

L

G

Y

T

S

S

A

R
|
R

E
x
C

K

P

P

E

F
|
F

K

K

K

E

G

-

V

-

S

Q

D

E

V

V

D

R

K

K

P

V

A

A

L

I

I

-

L

-

Q

E

N

N

I

L

Q

R

E

K

M

R

G

G

L

I

D

E

C

G

V

L

V

V

C

V

I

I

G

G

G
|
G

N

D

G
|
G

T
x
S

Q

Y

K
x
R

T
x
G

A

A

A

Q

K

R

F

I

A

S

-

E

A

C

M

K

G

E

V

I

N

Q

I

T

V

I

S

G

V

I

P

P

K

G

T

T

I

I

D

D

N

N

D

D

I

I

W

N

G

G

T

T

D

D

I

F

S

T

F

I

G

G

F

F

D

D

S

T

A

A

V

L

S

N

I

T

A

I

T

I

D

G

A

L

I

V

D

D

R

K

L

I

H

R

S

D

T

T

A

A

S

S

H

H

K

A

R

R

V

T

M

F

V

I

I

I

E

E

V

A

M

M

G

G

H

R

K

D

A

C

G

G

W

D

I

L

A

A

L

L

Y

W

S

A

G

G

M

L

A

S

G

V

G

G

G

A

D

E

V

T

I

I

L

V

V

V

P

P

E

E

I

V

P

K

Y

T

N

D

I

I

K

K

N

E

I

I

G

A

N

D

T

K

I

I

L

E

E

Q

R

G

L

I

K

K

K

R

G

G

K

K

P

K

Y

H

S

S

I

I

V

V

V

L

V

V

A

A

E

E

G

G

I

C

L

M

T

T

-

A

-

Q

D

D

G

C

R

Q

K

K

R

E

A

L

A

S

E

Q

Y

Y

I

I

A

-

Q

-

E

-

I

-

E

-

Y

-

E

-

T

-

G

N

I

V

E

D

T

N

R

R

E

V

T

S

V

V

L

L

G

G

Y

H

I

V

Q

Q

R

R

G

G

S

S

P

P

T

T

P

G

F

A

D

D

R

R

N

V

L

L

S

A

T

S

R

R

M

L

G

G

H

Y

A

A

T

V

E

D

L

L

I

L

A

M

N

Q

G

G

Q

E

F

T

G

A

R

K

M

G

I

V

A

G

L

I

K

K

G

N

D

N

D

K

I

I

S

V

S

A

I

T

P

S

L

F

E

D

E

E

V

I

binding adenosine-5'-diphosphate: S9 (= S8), Y41 (≠ F41), R72 (= R72), C73 (≠ E73), F76 (= F76), G102 (= G108), G104 (= G110), S105 (≠ T111), R107 (≠ K113), G108 (≠ T114)

Q2FXM8 ATP-dependent 6-phosphofructokinase; ATP-PFK; Phosphofructokinase; Phosphohexokinase; EC 2.7.1.11 from Staphylococcus aureus (strain NCTC 8325 / PS 47) (see paper)
39% identity, 92% coverage: 2:311/336 of query aligns to 3:301/322 of Q2FXM8

query
sites
Q2FXM8

R

K

I

I

G

A

I

V

L

L

T

T

S

S

G

G

D

D

C

S

P

P

G

G

I

M

N

N

A

A

T

A

I

V

R

R

G

A

V

V

C

V

K

R

T

T

A

A

I

I

N

-

H

Y

Y

N

G

E

M

I

E

E

V

V

I

Y

G

G

I

V

H

Y

S

H

G

G

F

Y

Q

Q

G

G

L

L

T

N

K

D

D

D

I

I

E

H

S

K

F

L

T

E

D

L

K

G

S

S

L

V

S

G

G

D

L

T

L

I

N

Q

Q

R

G

G

T

T

M

F

L

L

G

Y

T

S

S

A

R
|
R

E
x
C

K

P

P

E

F

F

K

K

K

E

G

-

V

-

S

Q

D

E

V

V

D

R

K

K

P

V

A

A

L

I

I

-

L

-

Q

E

N

N

I

L

Q

R

E

K

M

R

G

G

L

I

D

E

C

G

V

L

V

V

C

V

I

I

G

G

G
|
G

N
x
D

G
|
G

T
x
S

Q

Y

K

R

T

G

A

A

A

Q

K

R

F

I

A

S

-

E

A

C

M

K

G

E

V

I

N

Q

I

T

V

I

S

G

V

I

P

P

K

G

T
|
T

I
|
I

D
|
D

N

N

D

D

I

I

W

N

G

G

T

T

D

D

I

F

S

T

F

I

G

G

F

F

D

D

S

T

A

A

V

L

S

N

I

T

A

I

T

I

D
x
G

A
x
L

I

V

D

D

R

K

L

I

H

R

S

D

T

T

A

A

S

S

H

H

K

A

R
|
R

V

T

M

F

V

I

I

I

E

E

V

A

M
|
M

G
|
G

H
x
R

K

D

A

C

G

G

W

D

I

L

A

A

L

L

Y

W

S

A

G

G

M

L

A

S

G

V

G

G

G

A

D

E

V

T

I

I

L

V

V

V

P

P

E

E

I

V

P

K

Y

T

N

D

I

I

K

K

N

E

I

I

G

A

N

D

T

K

I

I

L

E

E

Q

R

G

L

I

K

K

K

R

G

G

K

K

P

K

Y

H

S

S

I

I

V

V

V

L

V

V

A

A

E
|
E

G

G

I

C

L

M

T

T

-

A

-

Q

D

D

G

C

R

Q

K

K

R

E

A

L

A

S

E

Q

Y

Y

I

I

A

-

Q

-

E

-

I

-

E

-

Y

-

E

-

T

-

G

N

I

V

E

D

T

N

R
|
R

E

V

T

S

V

V

L

L

G

G

Y
x
H

I
x
V

Q
|
Q

R
|
R

G

G

S

S

P

P

T

T

P

G

F

A

D

D

R

R

N

V

L

L

S

A

T

S

R

R

M

L

G

G

H

Y

A

A

T

V

E

D

L

L

I

L

A

M

N

Q

G

G

Q

E

F

T

G

A

R

K

M

G

I

V

A

G

L

I

K

K

G

N

D

N

D

K

I

I

S

V

S

A

I

T

P

S

L

F

E

D

E

E

V

I

RC 72:73 (≠ RE 72:73) binding
GDGS 102:105 (≠ GNGT 108:111) binding
TID 127:129 (= TID 131:133) binding in other chain
G150 (≠ D154) mutation to D: Exhibits higher affinity for fructose 6-phosphate and higher catalytic activity with a loss of dimer conversion; in association with A-151.
L151 (≠ A155) mutation to A: Exhibits higher affinity for fructose 6-phosphate and higher catalytic activity with a loss of tetramer-dimer conversion; in association with D-150.
R164 (= R168) mutation to A: Complete loss of fructose 6-phosphate binding.
MGR 171:173 (≠ MGH 175:177) binding in other chain
E224 (= E228) binding in other chain
R245 (= R255) mutation to A: Complete loss of fructose 6-phosphate binding.
HVQR 251:254 (≠ YIQR 261:264) binding in other chain

5xz9A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
39% identity, 92% coverage: 2:311/336 of query aligns to 3:301/321 of 5xz9A

query
sites
5xz9A

R

K

I

I

G

A

I

V

L

L

T

T

S
|
S

G

G

D

D

C

S

P

P

G

G

I

M

N

N

A

A

T

A

I

V

R

R

G

A

V

V

C

V

K

R

T

T

A

A

I

I

N

-

H

Y

Y

N

G

E

M

I

E

E

V

V

I

Y

G

G

I

V

H

Y

S

H

G

G

F
x
Y

Q

Q

G

G

L

L

T

N

K

D

D

D

I

I

E

H

S

K

F

L

T

E

D

L

K

G

S

S

L

V

S

G

G

D

L

T

L

I

N

Q

Q

R

G

G

T

T

M

F

L

L

G

Y

T

S

S

A

R

R

E
x
C

K

P

P

E

F
|
F

K
|
K

K

E

G

-

V

-

S

Q

D

E

V

V

D

R

K

K

P

V

A

A

L

I

I

-

L

-

Q

E

N

N

I

L

Q

R

E

K

M

R

G

G

L

I

D

E

C

G

V

L

V

V

C

V

I

I

G

G

G
|
G

N
x
D

G
|
G

T
x
S

Q

Y

K
x
R

T
x
G

A

A

A

Q

K

R

F

I

A

S

-

E

A

C

M

K

G

E

V

I

N

Q

I

T

V

I

S

G

V

I

P

P

K

G

T

T

I

I

D

D

N

N

D

D

I

I

W

N

G

G

T

T

D

D

I

F

S

T

F

I

G

G

F

F

D

D

S

T

A

A

V

L

S

N

I

T

A

I

T

I

D

G

A

L

I

V

D

D

R

K

L

I

H

R

S

D

T

T

A

A

S

S

H

H

K

A

R

R

V

T

M

F

V

I

I

I

E

E

V

A

M

M

G

G

H

R

K

D

A

C

G

G

W

D

I

L

A

A

L

L

Y

W

S

A

G

G

M

L

A

S

G

V

G

G

G

A

D

E

V

T

I

I

L

V

V

V

P

P

E

E

I

V

P

K

Y

T

N

D

I

I

K

K

N

E

I

I

G

A

N

D

T

K

I

I

L

E

E

Q

R

G

L

I

K

K

K

R

G

G

K

K

P

K

Y

H

S

S

I

I

V

V

V

L

V

V

A

A

E

E

G

G

I

C

L

M

T

T

-

A

-

Q

D

D

G

C

R

Q

K

K

R

E

A

L

A

S

E

Q

Y

Y

I

I

A

-

Q

-

E

-

I

-

E

-

Y

-

E

-

T

-

G

N

I

V

E

D

T

N

R

R

E

V

T

S

V

V

L

L

G

G

Y

H

I

V

Q

Q

R

R

G

G

S

S

P

P

T

T

P

G

F

A

D

D

R

R

N

V

L

L

S

A

T

S

R

R

M

L

G

G

H

Y

A

A

T

V

E

D

L

L

I

L

A

M

N

Q

G

G

Q

E

F

T

G

A

R

K

M

G

I

V

A

G

L

I

K

K

G

N

D

N

D

K

I

I

S

V

S

A

I

T

P

S

L

F

E

D

E

E

V

I

binding adenosine-5'-triphosphate: S9 (= S8), Y41 (≠ F41), C73 (≠ E73), F76 (= F76), K77 (= K77), G102 (= G108), D103 (≠ N109), G104 (= G110), S105 (≠ T111), R107 (≠ K113), G108 (≠ T114)

3o8lA Structure of phosphofructokinase from rabbit skeletal muscle (see paper)
33% identity, 96% coverage: 3:323/336 of query aligns to 10:355/748 of 3o8lA

query
sites
3o8lA

I

I

G

A

I

V

L

L

T

T

S

S

G
|
G

D

D

C

A

P

Q

G

G

I

M

N

N

A

A

T

A

I

V

R

R

G

A

V

V

C

V

K

R

T

V

A

G

I

I

N

-

H

F

Y

T

G

G

M

A

E

R

V

V

I

F

G

F

I

V

H

H

S

E

G

G

F
x
Y

Q

Q

G

G

L

L

L

V

T

D

-

G

K

D

D

H

I

I

E

R

S

E

F

A

T

T

D

W

K

E

S

S

L

V

S

S

G

M

L

M

L

L

N

Q

Q

L

G

G

T

T

M

V

L

I

G

G

T

S

S

A

R
|
R

E
x
C

K

K

P

D

F

F

K
x
R

K

-

G

-

V

-

S

-

D

E

V

R

D

E

K

G

P

R

A

L

L

R

I

A

L

A

Q

H

N

N

I

L

Q

V

E

K

M

R

G

G

L

I

D

T

C

N

V

L

V

C

C

V

I

I

G

G

G
|
G

N
x
D

G
|
G

T
x
S

Q

L

K
x
T

T
x
G

A

A

A

D

K

T

F

F

A

R

A

S

M

E

G

W

-

S

-

D

-

L

-

S

-

D

-

L

-

Q

-

K

-

A

-

G

-

K

-

I

-

T

-

A

-

E

-

A

-

T

-

R

-

S

-

Y

V

L

N

N

I

I

V

V

S

G

V

L

P

V

K

G

T
x
S

I

I

D
|
D

N

N

D
|
D

I

F

W

C

G

G

T

T

D
|
D

I
x
M

S

T

F

I

G

G

F

T

D

D

S

S

A

A

V

L

S

H

I

R

A

I

T

T

D

E

A

I

I

V

D

D

R

A

L

I

H

T

S

T

T

T

A

A

S

Q

S

S

H

H

K

Q

R

R

V

T

M

F

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x
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x
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x
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D

active site: G17 (= G10), R80 (= R72), D111 (≠ N109), S156 (≠ T131), D158 (= D133), D160 (= D135), R202 (≠ H177)
binding adenosine-5'-diphosphate: D165 (= D140), M166 (≠ I141), F300 (= F271)
binding adenosine-5'-triphosphate: G16 (= G9), Y47 (≠ F41), R80 (= R72), C81 (≠ E73), R85 (≠ K77), G110 (= G108), G112 (= G110), S113 (≠ T111), T115 (≠ K113), G116 (≠ T114), W219 (≠ V194), H234 (≠ T210), R238 (= R214)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 530, 663
binding adenosine-5'-triphosphate: 377, 382, 383
binding phosphate ion: 416, 449, 450, 463, 522, 524, 727

P00511 ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; PFK-M; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase; EC 2.7.1.11 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
33% identity, 96% coverage: 3:323/336 of query aligns to 18:363/780 of P00511

query
sites
P00511

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylthreonine
775 modified: Phosphoserine; by PKA

P08237 ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; PFK-M; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase; EC 2.7.1.11 from Homo sapiens (Human) (see 6 papers)
32% identity, 97% coverage: 3:329/336 of query aligns to 18:376/780 of P08237

query
sites
P08237

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x
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R39 (≠ K24) to P: in GSD7; Italian; dbSNP:rs121918193
G57 (= G43) to V: in GSD7; Italian
R100 (≠ L90) to Q: in dbSNP:rs2228500
S180 (= S147) to C: in GSD7; Italian
G209 (= G176) to D: in GSD7; loss of activity shown by complementation assays in yeast; dbSNP:rs767265360
D309 (= D272) to G: in GSD7; Spanish; complete loss of enzyme activity; dbSNP:rs1169383137

Sites not aligning to the query:

543 D → A: in GSD7; Italian; dbSNP:rs121918194
557 modified: N6-(2-hydroxyisobutyryl)lysine
591 D → A: in GSD7; Italian
686 W → C: in GSD7; Japanese; dbSNP:rs121918196
696 R → H: in dbSNP:rs41291971

P17858 ATP-dependent 6-phosphofructokinase, liver type; ATP-PFK; PFK-L; 6-phosphofructokinase type B; Phosphofructo-1-kinase isozyme B; PFK-B; Phosphohexokinase; EC 2.7.1.11 from Homo sapiens (Human) (see 3 papers)
32% identity, 96% coverage: 3:323/336 of query aligns to 18:363/780 of P17858

query
sites
P17858

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G81 (= G65) natural variant: G -> A
R151 (≠ A116) to W: in dbSNP:rs755851304

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
527 T→A: Does not affect GlcNAcylation.
529 modified: carbohydrate, O-linked (GlcNAc) serine; S→A: Prevents GlcNAcylation and enhance enzyme activity.

7lw1A Human phosphofructokinase-1 liver type bound to activator na-11 (see paper)
32% identity, 96% coverage: 3:323/336 of query aligns to 8:353/744 of 7lw1A

query
sites
7lw1A

I

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binding adenosine-5'-diphosphate: R78 (= R72), C79 (≠ E73), T83 (≠ S83), G108 (= G108), G110 (= G110), S111 (≠ T111), G114 (vs. gap), I117 (vs. gap)
binding 6-O-phosphono-beta-D-fructofuranose: I155 (= I132), D156 (= D133), R191 (= R168), M198 (= M175), R200 (≠ H177), E254 (= E228), R282 (= R255), H288 (≠ Y261), R291 (= R264)
binding N-{(11S)-2-[2-(5-hydroxypent-1-yn-1-yl)phenyl]-4H,10H-pyrazolo[5,1-c][1,4]benzoxazepin-7-yl}acetamide: M164 (≠ I141), F298 (= F271), I301 (≠ N274), K305 (≠ R278), N331 (≠ D301)

Sites not aligning to the query:

Q92448 ATP-dependent 6-phosphofructokinase subunit alpha; ATP-dependent 6-phosphofructokinase 1; ATP-PFK 1; Phosphofructokinase 1; Glucose-induced selective autophagy 1 protein; Phosphohexokinase 1; EC 2.7.1.11 from Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris) (see paper)
32% identity, 95% coverage: 2:320/336 of query aligns to 212:555/989 of Q92448

query
sites
Q92448

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D

D361 (= D133) mutation to S: Abolishes catalytic activity, but not the ability to modulate glucose-induced microautophagy of peroxisomes.

Q8TGA0 ATP-dependent 6-phosphofructokinase subunit beta; ATP-dependent 6-phosphofructokinase 2; ATP-PFK 2; Phosphofructokinase 2; Phosphohexokinase 2; EC 2.7.1.11 from Komagataella pastoris (Yeast) (Pichia pastoris) (see 2 papers)
32% identity, 91% coverage: 3:308/336 of query aligns to 184:516/941 of Q8TGA0

query
sites
Q8TGA0

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N

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I395 (= I195) binding
KPASSR 400:405 (≠ IPYNIK 200:205) binding
Q410 (≠ T210) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
557:558 binding

Query Sequence

>352883 FitnessBrowser__Btheta:352883
MRIGILTSGGDCPGINATIRGVCKTAINHYGMEVIGIHSGFQGLLTKDIESFTDKSLSGL
LNQGGTMLGTSREKPFKKGGVVSDVDKPALILQNIQEMGLDCVVCIGGNGTQKTAAKFAA
MGVNIVSVPKTIDNDIWGTDISFGFDSAVSIATDAIDRLHSTASSHKRVMVIEVMGHKAG
WIALYSGMAGGGDVILVPEIPYNIKNIGNTILERLKKGKPYSIVVVAEGILTDGRKRAAE
YIAQEIEYETGIETRETVLGYIQRGGSPTPFDRNLSTRMGGHATELIANGQFGRMIALKG
DDISSIPLEEVAGKLKLVTEDHDLVIQGRRMGICFG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory