SitesBLAST
Comparing 353219 FitnessBrowser__Btheta:353219 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 19 hits to proteins with known functional sites (download)
P38502 Acetate kinase; Acetokinase; EC 2.7.2.1 from Methanosarcina thermophila (see 5 papers)
53% identity, 100% coverage: 1:398/399 of query aligns to 1:397/408 of P38502
- N7 (= N7) mutation to A: Almost abolishes catalytic activity. Requires increased magnesium levels for activity. Strongly decreases affinity for acetate.; mutation to D: Almost abolishes catalytic activity. Strongly decreases affinity for acetate.
- S10 (= S10) mutation S->A,T: Strongly decreases catalytic activity. Strongly decreases affinity for acetate.
- S12 (= S12) mutation to A: Decreases catalytic activity. Strongly decreases affinity for acetate. Requires increased magnesium levels for enzyme activity.; mutation to T: Decreases catalytic activity. Strongly decreases affinity for acetate.
- K14 (= K14) mutation to A: Strongly decreases enzyme activity.; mutation to R: Reduces enzyme activity.
- R91 (= R91) mutation R->A,L: Decreases catalytic activity. Decreases affinity for acetate.
- V93 (= V93) mutation to A: Decreases affinity for acetate.
- L122 (= L122) mutation to A: Decreases affinity for acetate.
- D148 (= D148) active site, Proton donor/acceptor; mutation D->A,E,N: Abolishes catalytic activity. Decreases affinity for acetate, but not for ATP.
- F179 (= F179) mutation to A: Decreases affinity for acetate.
- N211 (= N211) mutation to A: Slightly reduced enzyme activity.
- P232 (= P232) mutation to A: Decreases affinity for acetate.
- R241 (= R241) mutation R->K,L: Decreases catalytic activity. Strongly reduced affinity for ATP.
- E384 (= E385) mutation to A: Almost abolishes catalytic activity. Strongly decreases affinity for acetate. Requires strongly increased magnesium levels for enzyme activity.
1tuuA Acetate kinase crystallized with atpgs (see paper)
53% identity, 100% coverage: 1:398/399 of query aligns to 1:397/399 of 1tuuA
- active site: N7 (= N7), R91 (= R91), H180 (= H180), R241 (= R241), E384 (= E385)
- binding adenosine-5'-diphosphate: K14 (= K14), G210 (= G210), D283 (= D283), F284 (≠ M284), R285 (= R285), G331 (= G331), I332 (≠ V332), N335 (= N335)
- binding sulfate ion: R91 (= R91), H180 (= H180), G212 (= G212)
1tuuB Acetate kinase crystallized with atpgs (see paper)
53% identity, 100% coverage: 1:398/399 of query aligns to 1:397/398 of 1tuuB
- active site: N7 (= N7), R91 (= R91), H180 (= H180), R241 (= R241), E384 (= E385)
- binding adenosine monophosphate: D283 (= D283), R285 (= R285), G331 (= G331), I332 (≠ V332), N335 (= N335), S336 (≠ Q336)
- binding trihydrogen thiodiphosphate: H180 (= H180), G212 (= G212), R241 (= R241)
7fj9A Kpacka (pduw) with amppnp complex structure
44% identity, 99% coverage: 2:396/399 of query aligns to 3:391/395 of 7fj9A
7fj8A Kpacka (pduw) with amp complex structure
44% identity, 99% coverage: 2:396/399 of query aligns to 3:391/395 of 7fj8A
4fwsA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with ctp (see paper)
43% identity, 99% coverage: 3:396/399 of query aligns to 4:389/394 of 4fwsA
- active site: N8 (= N7), R83 (= R91), H172 (= H180), R233 (= R241), E378 (= E385)
- binding cytidine-5'-triphosphate: G202 (= G210), N203 (= N211), G204 (= G212), D275 (= D283), L276 (≠ M284), R277 (= R285), G323 (= G331), I324 (≠ V332), N327 (= N335)
- binding 1,2-ethanediol: V21 (≠ M20), C24 (≠ K23), H115 (= H123), N203 (= N211), T232 (= T240), R233 (= R241), K262 (= K270)
4fwrA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with cmp (see paper)
43% identity, 99% coverage: 3:396/399 of query aligns to 4:389/394 of 4fwrA
- active site: N8 (= N7), R83 (= R91), H172 (= H180), R233 (= R241), E378 (= E385)
- binding cytidine-5'-monophosphate: G202 (= G210), N203 (= N211), D275 (= D283), L276 (≠ M284), R277 (= R285), G323 (= G331), I324 (≠ V332), N327 (= N335)
4fwqA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gtp (see paper)
43% identity, 99% coverage: 3:396/399 of query aligns to 4:389/394 of 4fwqA
- active site: N8 (= N7), R83 (= R91), H172 (= H180), R233 (= R241), E378 (= E385)
- binding guanosine-5'-triphosphate: H172 (= H180), N203 (= N211), G204 (= G212), D275 (= D283), L276 (≠ M284), R277 (= R285), E280 (≠ L288), G323 (= G331), I324 (≠ V332), N327 (= N335)
4fwpA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gdp (see paper)
43% identity, 99% coverage: 3:396/399 of query aligns to 4:389/394 of 4fwpA
- active site: N8 (= N7), R83 (= R91), H172 (= H180), R233 (= R241), E378 (= E385)
- binding 1,2-ethanediol: S11 (= S10), H115 (= H123), K262 (= K270)
- binding guanosine-5'-diphosphate: N203 (= N211), D275 (= D283), L276 (≠ M284), R277 (= R285), E280 (≠ L288), G323 (= G331), I324 (≠ V332), N327 (= N335), S328 (≠ Q336)
4fwoA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gmp (see paper)
43% identity, 99% coverage: 3:396/399 of query aligns to 4:389/394 of 4fwoA
- active site: N8 (= N7), R83 (= R91), H172 (= H180), R233 (= R241), E378 (= E385)
- binding guanosine-5'-monophosphate: G202 (= G210), N203 (= N211), D275 (= D283), L276 (≠ M284), R277 (= R285), E280 (≠ L288), G323 (= G331), I324 (≠ V332), N327 (= N335)
- binding 1,2-ethanediol: E100 (= E108), N104 (≠ A112)
4fwnA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with adenosine tetraphosphate (ap4) (see paper)
43% identity, 99% coverage: 3:396/399 of query aligns to 4:389/394 of 4fwnA
- active site: N8 (= N7), R83 (= R91), H172 (= H180), R233 (= R241), E378 (= E385)
- binding adenosine-5'-tetraphosphate: H172 (= H180), H200 (= H208), N203 (= N211), G204 (= G212), D275 (= D283), L276 (≠ M284), R277 (= R285), G323 (= G331), I324 (≠ V332), N327 (= N335)
4fwmA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with atp (see paper)
43% identity, 99% coverage: 3:396/399 of query aligns to 4:389/394 of 4fwmA
- active site: N8 (= N7), R83 (= R91), H172 (= H180), R233 (= R241), E378 (= E385)
- binding adenosine-5'-triphosphate: H172 (= H180), H200 (= H208), N203 (= N211), G204 (= G212), D275 (= D283), L276 (≠ M284), R277 (= R285), G323 (= G331), I324 (≠ V332), N327 (= N335)
- binding 1,2-ethanediol: H172 (= H180), R233 (= R241)
4fwkA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with amp (see paper)
43% identity, 99% coverage: 3:396/399 of query aligns to 4:389/394 of 4fwkA
- active site: N8 (= N7), R83 (= R91), H172 (= H180), R233 (= R241), E378 (= E385)
- binding adenosine monophosphate: G202 (= G210), N203 (= N211), D275 (= D283), L276 (≠ M284), R277 (= R285), G323 (= G331), I324 (≠ V332), N327 (= N335)
- binding 1,2-ethanediol: D103 (≠ E111), N104 (≠ A112), R107 (≠ A115)
2e1zA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with diadenosine tetraphosphate (ap4a) obtained after co- crystallization with atp (see paper)
43% identity, 99% coverage: 3:396/399 of query aligns to 4:389/394 of 2e1zA
- active site: N8 (= N7), R83 (= R91), H172 (= H180), R233 (= R241), E378 (= E385)
- binding bis(adenosine)-5'-tetraphosphate: N8 (= N7), R83 (= R91), H115 (= H123), G202 (= G210), N203 (= N211), G204 (= G212), P224 (= P232), R233 (= R241), D275 (= D283), L276 (≠ M284), R277 (= R285), G323 (= G331), I324 (≠ V332), N327 (= N335)
1x3nA Crystal structure of amppnp bound propionate kinase (tdcd) from salmonella typhimurium (see paper)
43% identity, 99% coverage: 3:396/399 of query aligns to 4:389/394 of 1x3nA
- active site: N8 (= N7), R83 (= R91), H172 (= H180), R233 (= R241), E378 (= E385)
- binding phosphoaminophosphonic acid-adenylate ester: G202 (= G210), N203 (= N211), G204 (= G212), D275 (= D283), L276 (≠ M284), R277 (= R285), G323 (= G331), I324 (≠ V332), N327 (= N335)
1x3mA Crystal structure of adp bound propionate kinase (tdcd) from salmonella typhimurium (see paper)
43% identity, 99% coverage: 3:396/399 of query aligns to 4:389/394 of 1x3mA
- active site: N8 (= N7), R83 (= R91), H172 (= H180), R233 (= R241), E378 (= E385)
- binding adenosine-5'-diphosphate: G202 (= G210), N203 (= N211), D275 (= D283), L276 (≠ M284), R277 (= R285), G322 (= G330), G323 (= G331), I324 (≠ V332), N327 (= N335)
4ijnA Crystal structure of an acetate kinase from mycobacterium smegmatis bound to amp and sulfate (see paper)
41% identity, 98% coverage: 1:390/399 of query aligns to 2:370/376 of 4ijnA
- active site: N8 (= N7), R72 (= R91), H161 (= H180), R222 (= R241), E365 (= E385)
- binding adenosine monophosphate: G191 (= G210), N192 (= N211), D263 (= D283), F264 (≠ M284), R265 (= R285), G311 (= G331), V312 (= V332), N315 (= N335), V316 (≠ Q336)
4iz9A Crystal structure of an acetate kinase from mycobacterium avium bound to an unknown acid-apcpp conjugate and manganese (see paper)
40% identity, 99% coverage: 2:397/399 of query aligns to 5:379/381 of 4iz9A
- active site: N10 (= N7), R74 (= R91), H163 (= H180), R224 (= R241), E367 (= E385)
- binding diphosphomethylphosphonic acid adenosyl ester: K17 (= K14), G193 (= G210), N194 (= N211), D265 (= D283), F266 (≠ M284), R267 (= R285), G313 (= G331), I314 (≠ V332), N317 (= N335), D318 (≠ Q336)
1sazA Membership in the askha superfamily: enzymological properties and crystal structure of butyrate kinase 2 from thermotoga maritima (see paper)
28% identity, 44% coverage: 175:351/399 of query aligns to 149:324/375 of 1sazA
- active site: R214 (= R241)
- binding phosphomethylphosphonic acid adenylate ester: H154 (= H180), G184 (= G210), G185 (≠ N211), G186 (= G212), S254 (= S282), D255 (= D283), A256 (≠ M284), R257 (= R285), G304 (= G331), L305 (≠ V332), H307 (≠ E334)
Query Sequence
>353219 FitnessBrowser__Btheta:353219
MKILVLNCGSSSIKYKLFDMTTKEVIAQGGIEKIGLKGSFLKLTLPNGEKKVLEKDIPEH
TIGVEFILNTLISPEYGAIKSLDEINAVGHRMVHGGERFSESVLLNKEVLEAFAACNDLA
PLHNPANLKGVNAVSAILPNIPQVGVFDTAFHQTMPDYAYMYAIPYEMYEKYGVRRYGFH
GTSHRYVSKRVCEFLGVNPVGQKIITCHIGNGGSIAAIKDGKCIDTTMGLTPLEGLMMGT
RSGDIDAGAVTFIMEKEGLNTTGISNLLNKKSGVLGISGVSSDMRELLAACANGNERAIL
AEKMYYYRIKKYIGAYAAALGGVDIILFTGGVGENQFECRESVCKDMEFMGIKLDNNVNA
KVRGEEAIISTADSKVKVVVIPTDEELLIASDTMDILKK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory