SitesBLAST
Comparing 353265 FitnessBrowser__Btheta:353265 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 13 hits to proteins with known functional sites (download)
7t9gA Structure of vcindy-na+ (see paper)
31% identity, 85% coverage: 66:495/504 of query aligns to 32:442/445 of 7t9gA
6wtxA Structure of vcindy in complex with terephthalate (see paper)
31% identity, 85% coverage: 66:495/504 of query aligns to 32:442/445 of 6wtxA
- binding sodium ion: S129 (= S182), S133 (= S186), N134 (= N187), G176 (= G227), G182 (= G233), T356 (≠ A405), A359 (≠ I408), S360 (= S409), N361 (≠ H410), A403 (≠ S458)
- binding terephthalic acid: N134 (= N187), S183 (≠ T234), S360 (= S409), N361 (≠ H410), T362 (= T411), T404 (= T459)
6okzB Structure of vcindy bound to fumarate
31% identity, 85% coverage: 66:495/504 of query aligns to 31:441/444 of 6okzB
8r34A Cryoem structure of the symmetric pho90 dimer from yeast with substrates. (see paper)
27% identity, 87% coverage: 62:499/504 of query aligns to 47:475/485 of 8r34A
8w6nB Nas1 with sulfate in in/out state (see paper)
28% identity, 73% coverage: 122:488/504 of query aligns to 68:455/481 of 8w6nB
8w6nA Nas1 with sulfate in in/out state (see paper)
28% identity, 73% coverage: 122:488/504 of query aligns to 72:467/494 of 8w6nA
8w6hA Nas1 with sulfate - in/in state (see paper)
29% identity, 73% coverage: 122:488/504 of query aligns to 67:436/462 of 8w6hA
7jsjA Structure of the nact-pf2 complex (see paper)
27% identity, 84% coverage: 64:488/504 of query aligns to 25:443/468 of 7jsjA
- binding sodium ion: S124 (= S182), I127 (≠ L185), S128 (= S186), N129 (= N187), G172 (= G233), T366 (≠ A405), T369 (≠ I408), S370 (= S409), N371 (≠ H410), A413 (≠ S458), T414 (= T459)
- binding (2R)-2-[2-(4-tert-butylphenyl)ethyl]-2-hydroxybutanedioic acid: N129 (= N187), T130 (= T188), T173 (= T234), G315 (≠ S355), I316 (≠ V356), N371 (≠ H410)
4f35B Crystal structure of a bacterial dicarboxylate/sodium symporter (see paper)
30% identity, 85% coverage: 66:495/504 of query aligns to 31:411/414 of 4f35B
Q86YT5 Na(+)/citrate cotransporter; NaCT; Sodium-coupled citrate transporter; Sodium-dependent citrate transporter; Solute carrier family 13 member 5 from Homo sapiens (Human) (see 5 papers)
25% identity, 84% coverage: 64:488/504 of query aligns to 37:537/568 of Q86YT5
- T142 (= T188) to M: in DEE25; no loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs761917087
- G219 (= G226) to E: loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs150024888; to R: in DEE25; loss of function in citrate transport; loss of localization to plasma membrane; dbSNP:rs144332569
- T227 (= T234) to M: in DEE25; loss of function in citrate transport; no effect on localization to plasma membrane; dbSNP:rs587777577
- D243 (≠ E248) to N: no effect on localization to plasma membrane; no effect on its function in citrate transport; dbSNP:rs142262032
- G409 (≠ S355) mutation to Q: No effect on its function in citrate transport.
- I410 (≠ V356) mutation I->A,F: Significant loss of function in citrate transport.; mutation to V: No effect on its function in citrate transport.
- L420 (= L366) to P: loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs150738356
- S427 (≠ T373) to L: in DEE25; loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs548065551
- L485 (= L432) to R: no effect on localization to plasma membrane; reduced function in citrate transport; increased Km and Vmax values compared with that of wild type with citrate as substrate; dbSNP:rs148049520
- L488 (= L435) to P: in DEE25; loss of function in citrate transport; loss of localization to plasma membrane; dbSNP:rs587777578
- D524 (= D475) to H: in DEE25; loss of function in citrate transport; no effect on localization to plasma membrane; dbSNP:rs863225448
Sites not aligning to the query:
- 341:568 natural variant: Missing (in DEE25; loss of localization to plasma membrane; loss of function in citrate transport)
- 562 modified: carbohydrate, N-linked (GlcNAc...) asparagine
8w6cA Cryoem structure of nadc1 with citrate (see paper)
26% identity, 71% coverage: 134:490/504 of query aligns to 88:502/530 of 8w6cA
Sites not aligning to the query:
8w6gA Nadc1 with inhibitor aca (see paper)
26% identity, 71% coverage: 134:490/504 of query aligns to 88:499/527 of 8w6gA
Sites not aligning to the query:
Q28615 Solute carrier family 13 member 2; Na(+)/dicarboxylate cotransporter 1; NaDC-1; Renal sodium/dicarboxylate cotransporter from Oryctolagus cuniculus (Rabbit) (see paper)
25% identity, 71% coverage: 134:490/504 of query aligns to 88:553/593 of Q28615
- Y228 (≠ V221) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity.
- Y432 (≠ F364) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity. Decreases Km value for succinate. More sensitive to inhibition by lithium.
- T474 (≠ I408) mutation to C: Does not affect cell membrane localization. Abolishes succinate transport activity.
- N525 (= N462) mutation to C: Decreases cell membrane expression. Decreases succinate transport activity.
- M539 (= M476) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity. Insensitive to inhibition by lithium.
Sites not aligning to the query:
- 83 L→C: Decreases cell membrane expression. Decreases succinate transport activity. Decreases Km value for succinate.
- 86 T→C: Does not affect cell membrane localization. Decreases succinate transport activity.
Query Sequence
>353265 FitnessBrowser__Btheta:353265
MYKIFHGFHLVEAYQDLKKAKRLAKNQTVARCIKLTIAITLSLILWFLPIDTFGIEGLTV
IEQRLISIFIFATLMWVFEAVPAWTTSVLIVVLLLLTVSDSSLWFLTQNTPAEELGQTVK
YKSILHCFADPIIMLFIGGFILAIAATKSGLDVLLARVMLRPFGTQSRYVLLGFILVTAV
FSMFLSNTATAAMMLTFLTPVLKALPADGKGKIGLAMAIPVAANVGGMGTPIGTPPNAIA
LKYLNDPEGLNLNIGFGEWMSFMLPYTIIVLFIAWFILLRLFPFKQKSIELQIEGEAKKD
WRSIVVYITFAITVLLWMFDKFTGVNSNVVAMIPVAVFCITGVITKRDLEEISWSVLWMV
AGGFALGVALQETGLAKHMIEAIPFSTWPPVLMIVGSGLICYAMANFISHTATAALLVPI
LAIAGISMRENLSSLGGVETLLIGVAIGSSLAMILPISTPPNALAHATGMIQQKDMEKVG
IIMGIIGLILGYTMLIILGSNKLL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory