SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 353284 BT3758 acetylornithine aminotransferase (NCBI ptt file) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5SHH5 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
38% identity, 99% coverage: 6:373/373 of query aligns to 23:394/395 of Q5SHH5

query
sites
Q5SHH5

V

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GT 113:114 (≠ GA 96:97) binding
K254 (= K237) modified: N6-(pyridoxal phosphate)lysine
T283 (= T264) binding

1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
38% identity, 99% coverage: 6:373/373 of query aligns to 15:386/387 of 1wkhA

query
sites
1wkhA

V

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active site: F132 (= F123), E184 (= E175), D217 (= D208), Q220 (= Q211), K246 (= K237), T275 (= T264), R363 (= R350)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (≠ H37), S104 (= S95), G105 (= G96), T106 (≠ A97), N109 (= N100), F132 (= F123), S133 (≠ H124), G134 (= G125), E184 (= E175), E189 (≠ V180), D217 (= D208), I219 (= I210), Q220 (= Q211), K246 (= K237), T274 (= T263), T275 (= T264), R363 (= R350)

Sites not aligning to the query:

active site: 13

1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
38% identity, 99% coverage: 6:373/373 of query aligns to 15:386/387 of 1wkgA

query
sites
1wkgA

V

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active site: F132 (= F123), E184 (= E175), D217 (= D208), Q220 (= Q211), K246 (= K237), T275 (= T264), R363 (= R350)
binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y16 (= Y7), Y46 (≠ H37), S104 (= S95), G105 (= G96), T106 (≠ A97), N109 (= N100), F132 (= F123), S133 (≠ H124), G134 (= G125), R135 (= R126), E184 (= E175), E189 (≠ V180), D217 (= D208), I219 (= I210), Q220 (= Q211), K246 (= K237), G273 (= G262), T274 (= T263), T275 (= T264)

Sites not aligning to the query:

active site: 13

1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
38% identity, 99% coverage: 6:373/373 of query aligns to 15:386/387 of 1vefA

query
sites
1vefA

V

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L

G

P

Y

P

E

E

D

L

Y

N

S

R

L

V

F

F

L

P

I

V

N

N

S
|
S

G
|
G

A
x
T

E

E

A

A

N
|
N

E

E

N

A

A

A

L

L

K

K

L

F

A

A

S

R

F

A

Y

H

N

T

G

G

R

R

T

K

K

K

V

F

I

V

S

A

F

A

S

M

K

R

A

G

F
|
F

H
x
S

G
|
G

R

R

T

T

S

M

L

G

A

S

V

L

E

S

A

V

T

T

N

W

N

E

P

P

T

K

I

Y

I

R

A

E

P

P

-

F

I

L

N

P

N

L

N

V

G

E

H

P

V

V

T

E

Y

F

L

I

P

P

L

Y

N

N

D

D

I

V

E

E

A

A

M

L

K

K

Q

R

E

A

L

V

A

D

K

E

G

-

D

E

V

T

C

A

A

A

V

V

I

I

I

L

E
|
E

G

P

I

V

Q

Q

G

G

V

E

G

G

G

G

I

V

K

R

I

P

P

A

T

T

T

P

E

E

F

F

M

L

Q

R

E

A

L

A

R

R

K

E

V

I

C

T

T

Q

E

E

T

K

G

G

T

A

I

L

L

L

I

I

L

L

D
|
D

E

E

I
|
I

Q
|
Q

S

T

G

G

Y

M

G

G

R

R

S

T

G

G

K

K

F

R

F

F

A

A

H

F

Q

E

Y

H

N

F

H

G

I

I

Q

V

P

P

D

D

I

I

I

L

T

T

V

L

A

A

K
|
K

G

A

I

L

G

G

N

G

G

G

F

V

P

P

M

L

A

G

-

V

G

A

V

V

L

M

I

R

S

E

P

E

M

V

F

A

K

R

P

S

V

M

-

P

Y

K

G

G

Q

G

L

H

G

G

T
|
T

T
|
T

F

F

G

G

G

G

N

N

H

P

L

L

A

A

C

M

S

A

A

A

A

G

L

V

A

A

V

A

M

I

D

R

V

Y

I

L

E

E

Q

R

D

T

N

R

L

L

V

W

E

E

N

R

A

A

K

A

A

E

V

L

G

G

D

P

Y

W

L

F

L

M

E

E

E

K

L

L

K

R

K

A

F

I

-

P

-

S

P

P

Q

K

I

I

K

R

E

E

V

V

R

R

G

G

R

M

G

G

L

L

M

M

I

V

G

G

L

L

E

E

F

L

E

K

E

E

P

K

I

A

K

A

E

P

L

Y

R

I

S

A

R

R

L

L

I

E

Y

K

D

E

E

H

H

R

V

V

F

L

T

A

G

L

A

Q

S

A

G

G

T

P

N

T

V

V

L

I

R
|
R

L

F

L

L

P

P

P

P

L

L

C

V

L

I

S

E

M

K

E

E

E

D

A

L

D

E

E

R

F

V

L

V

A

E

R

A

F

V

K

R

R

A

V

V

L

L

active site: F132 (= F123), D217 (= D208), K246 (= K237), T275 (= T264), R363 (= R350)
binding pyridoxal-5'-phosphate: S104 (= S95), G105 (= G96), T106 (≠ A97), N109 (= N100), F132 (= F123), S133 (≠ H124), G134 (= G125), E184 (= E175), D217 (= D208), I219 (= I210), Q220 (= Q211), K246 (= K237), T274 (= T263), T275 (= T264)

Sites not aligning to the query:

active site: 13

2ordA Crystal structure of acetylornithine aminotransferase (ec 2.6.1.11) (acoat) (tm1785) from thermotoga maritima at 1.40 a resolution
36% identity, 100% coverage: 1:373/373 of query aligns to 9:390/393 of 2ordA

query
sites
2ordA

M

M

K

Y

L

L

F

M

D

N

V

T

Y

Y

P

S

L

R

Y

F

D

P

I

A

N

T

I

F

V

V

K

Y

G

G

Q

K

G

G

C

S

K

W

V

I

W

Y

D

D

E

E

N

K

G

G

T

N

E

A

Y

Y

L

L

D

D

L

F

Y

T

G

S

G

G

H

I

A

A

V

V

I

N

S

V

I

L

G

G

H

H

A

S

H

H

P

P

H

R

Y

L

V

V

E

E

M

A

I

I

S

K

N

D

Q

Q

V

A

A

E

T

K

L

L

G

I

F

H

Y

C

S

S

N

N

S

L

V

F

I

W

N

N

K

R

L

P

Q

Q

Q

M

Q

E

V

L

A

A

E

E

R

L

L

L

G

S

K

K

-

N

-

T

I

F

S

G

G

G

Y

-

E

-

D

-

Y

-

S

K

L

V

F

F

L

F

I

A

N

N

S
x
T

G
|
G

A
x
T

E

E

A

A

N
|
N

E

E

N

A

A

A

L

I

K

K

L

I

A

A

S

R

F

K

Y

Y

N

G

G

K

R

K

T

K

-

S

-

E

-

K

-

K

-

Y

K

R

V

I

I

L

S

S

F

A

S

H

K

N

A

S

F
|
F

H
|
H

G
|
G

R

R

T

T

S

L

L

G

A

S

V

L

E

T

A

A

T

T

N

G

N

Q

P

P

T

K

I

Y

I

Q

A

K

P

P

I

F

N

E

N

P

-

L

N

V

G

P

H

G

V

F

T

E

Y

Y

L

F

P

E

L

F

N

N

D

N

I

V

E

E

A

D

M

L

K

R

Q

R

E

K

L

M

A

S

K

E

G

-

D

D

V

V

C

C

A

A

V

V

I

F

I

L

E
|
E

G

P

I

I

Q

Q

G

G

V
x
E

G

S

G

G

I

I

K

V

I

P

P

A

T

T

T

K

E

E

F

F

M

L

Q

E

E

E

L

A

R

R

K

K

V

L

C

C

T

D

E

E

T

Y

G

D

T

A

I

L

L

L

I

V

L

F

D
|
D

E

E

I
x
V

Q
|
Q

S

C

G

G

Y

M

G

G

R

R

S

T

G

G

K

K

F

L

F

F

A

A

H

Y

Q

Q

Y

K

N

Y

H

G

I

V

Q

V

P

P

D

D

I

V

I

L

T

T

V

T

A

A

K
|
K

G

G

I

L

G

G

N

G

G

G

F

V

P

P

M

I

A

G

G

A

V

V

L

I

I

V

S

N

P

E

-

R

-

A

-

N

M

V

F

L

K

E

P

P

V

-

Y

-

G

G

Q

D

L

H

G

G

T
|
T

T
|
T

F

F

G

G

G

G

N

N

H

P

L

L

A

A

C

C

S

R

A

A

A

G

L

V

A

T

V

V

M

I

D

K

V

E

I

L

E

T

Q

K

D

E

N

G

L

F

V

L

E

E

N

E

A

V

K

E

A

E

V

K

G

G

D

N

Y

Y

L

L

L

M

E

K

E

K

L

L

K

Q

K

E

F

M

P

K

Q

E

-

E

-

Y

-

D

-

V

I

V

K

A

E

D

V

V

R

R

G

G

R

M

G

G

L

L

M

M

I

I

G

G

L

I

E

Q

F

F

E

R

E

E

P

E

I

V

-

S

-

N

K

R

E

E

L

V

R

A

S

T

R

K

L

-

I

C

Y

F

D

E

E

N

H

K

V

L

F

L

T

V

G

V

A

P

S

A

G

G

T

N

N

N

V

T

L

I

R
|
R

L

F

L

L

P

P

P

P

L

L

C

T

L

V

S

E

M

Y

E

G

E

E

A

I

D

D

E

L

F

A

L

V

A

E

R

T

F

L

K

K

R

K

V

V

L

L

active site: F134 (= F123), E186 (= E175), D219 (= D208), Q222 (= Q211), K248 (= K237), T276 (= T264), R367 (= R350)
binding pyridoxal-5'-phosphate: T101 (≠ S95), G102 (= G96), T103 (≠ A97), N106 (= N100), F134 (= F123), H135 (= H124), G136 (= G125), E186 (= E175), E191 (≠ V180), D219 (= D208), V221 (≠ I210), Q222 (= Q211), K248 (= K237), T275 (= T263), T276 (= T264)

Q9X2A5 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
36% identity, 100% coverage: 1:373/373 of query aligns to 1:382/385 of Q9X2A5

query
sites
Q9X2A5

M

M

K

Y

L

L

F

M

D

N

V

T

Y

Y

P

S

L

R

Y

F

D

P

I

A

N

T

I

F

V

V

K

Y

G

G

Q

K

G

G

C

S

K

W

V

I

W

Y

D

D

E

E

N

K

G

G

T

N

E

A

Y

Y

L

L

D

D

L

F

Y

T

G

S

G

G

H

I

A

A

V

V

I

N

S

V

I

L

G

G

H

H

A

S

H

H

P

P

H

R

Y

L

V

V

E

E

M

A

I

I

S

K

N

D

Q

Q

V

A

A

E

T

K

L

L

G

I

F

H

Y

C

S

S

N

N

S

L

V

F

I

W

N

N

K

R

L

P

Q

Q

Q

M

Q

E

V

L

A

A

E

E

R

L

L

L

G

S

K

K

-

N

-

T

I

F

S

G

G

G

Y

-

E

-

D

-

Y

-

S

K

L

V

F

F

L

F

I

A

N

N

S

T

G
|
G

A
x
T

E

E

A

A

N

N

E

E

N

A

A

A

L

I

K

K

L

I

A

A

S

R

F

K

Y

Y

N

G

G

K

R

K

T

K

-

S

-

E

-

K

-

K

-

Y

K

R

V

I

I

L

S

S

F

A

S

H

K

N

A

S

F

F

H

H

G

G

R

R

T

T

S

L

L

G

A

S

V

L

E

T

A

A

T

T

N

G

N

Q

P

P

T

K

I

Y

I

Q

A

K

P

P

I

F

N

E

N

P

-

L

N

V

G

P

H

G

V

F

T

E

Y

Y

L

F

P

E

L

F

N

N

D

N

I

V

E

E

A

D

M

L

K

R

Q

R

E

K

L

M

A

S

K

E

G

-

D

D

V

V

C

C

A

A

V

V

I

F

I

L

E

E

G

P

I

I

Q

Q

G

G

V

E

G

S

G

G

I

I

K

V

I

P

P

A

T

T

T

K

E

E

F

F

M

L

Q

E

E

E

L

A

R

R

K

K

V

L

C

C

T

D

E

E

T

Y

G

D

T

A

I

L

L

L

I

V

L

F

D

D

E

E

I

V

Q

Q

S

C

G

G

Y

M

G

G

R

R

S

T

G

G

K

K

F

L

F

F

A

A

H

Y

Q

Q

Y

K

N

Y

H

G

I

V

Q

V

P

P

D

D

I

V

I

L

T

T

V

T

A

A

K

K

G

G

I

L

G

G

N

G

G

G

F

V

P

P

M

I

A

G

G

A

V

V

L

I

I

V

S

N

P

E

-

R

-

A

-

N

M

V

F

L

K

E

P

P

V

-

Y

-

G

G

Q

D

L

H

G

G

T

T

T
|
T

F

F

G

G

G

G

N

N

H

P

L

L

A

A

C

C

S

R

A

A

A

G

L

V

A

T

V

V

M

I

D

K

V

E

I

L

E

T

Q

K

D

E

N

G

L

F

V

L

E

E

N

E

A

V

K

E

A

E

V

K

G

G

D

N

Y

Y

L

L

L

M

E

K

E

K

L

L

K

Q

K

E

F

M

P

K

Q

E

-

E

-

Y

-

D

-

V

I

V

K

A

E

D

V

V

R

R

G

G

R

M

G

G

L

L

M

M

I

I

G

G

L

I

E

Q

F

F

E

R

E

E

P

E

I

V

-

S

-

N

K

R

E

E

L

V

R

A

S

T

R

K

L

-

I

C

Y

F

D

E

E

N

H

K

V

L

F

L

T

V

G

V

A

P

S

A

G

G

T

N

N

N

V

T

L

I

R

R

L

F

L

L

P

P

P

P

L

L

C

T

L

V

S

E

M

Y

E

G

E

E

A

I

D

D

E

L

F

A

L

V

A

E

R

T

F

L

K

K

R

K

V

V

L

L

GT 94:95 (≠ GA 96:97) binding
T268 (= T264) binding

O66442 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Aquifex aeolicus (strain VF5)
36% identity, 99% coverage: 3:373/373 of query aligns to 4:376/376 of O66442

query
sites
O66442

L

L

F

M

D

N

V

N

Y

Y

P

A

L

R

Y

L

D

P

I

V

N

K

I

F

V

V

K

R

G

G

Q

K

G

G

C

V

K

Y

V

L

W

Y

D

D

E

E

N

E

G

G

T

K

E

E

Y

Y

L

L

D

D

L

F

Y

V

G

S

G

G

H

I

A

G

V

V

I

N

S

S

I

L

G

G

H

H

A

A

H

Y

P

P

H

K

Y

L

V

T

E

E

M

A

I

L

S

K

N

E

Q

Q

V

V

A

E

T

K

L

L

G

L

F

H

Y

V

S

S

N

N

S

L

V

Y

I

E

N

N

K

P

L

W

Q

Q

Q

E

Q

E

V

L

A

A

E

H

R

K

L

L

G

V

K

K

I

-

S

H

G

F

Y

W

E

T

D

E

Y

G

S

K

L

V

F

F

L

F

I

A

N

N

S

S

G
|
G

A
x
T

E

E

A

S

N

V

E

E

N

A

A

A

L

I

K

K

L

L

A

A

S

R

F

K

Y

Y

-

W

-

R

-

D

-

K

-

G

N

K

G

N

R

K

T

W

K

K

V

F

I

I

S

S

F

F

S

E

K

N

A

S

F

F

H

H

G

G

R

R

T

T

S

Y

L

G

A

S

V

L

E

S

A

A

T

T

N

G

N

Q

P

P

T

K

I

F

-

H

-

K

-

G

I

F

A

E

P

P

I

L

N

V

N

P

N

G

G

-

H

-

V

F

T

S

Y

Y

L

A

P

K

L

L

N

N

D

D

I

I

E

D

A

S

M

V

K

Y

Q

K

E

L

L

L

A

D

K

E

G

-

D

E

V

T

C

A

A

G

V

I

I

I

I

I

E

E

G

V

I

I

Q

Q

G

G

V

E

G

G

G

G

I

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K

N

I

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P

A

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T

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E

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F

F

M

L

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L

L

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C

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L

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D

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G

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R

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F

F

Y

A

A

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Y

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Q

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F

H

N

I

L

Q

K

P

P

D

D

I

V

I

I

T

A

V

L

A

A

K
|
K

G

G

I

L

G

G

N

G

G

G

F

V

P

P

M

I

A

G

G

A

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I

L

L

-

A

-

R

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-

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I

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F

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Y

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G

G

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T
|
T

F

F

G

G

G

G

N

N

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P

L

L

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A

C

C

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A

A

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L

T

A

V

V

V

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D

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N

-

L

L

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A

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A

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G

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Y

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K

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L

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L

L

M

M

I

L

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G

L

L

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F

L

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P

E

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C

K

K

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L

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L

R

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L

A

I

L

Y

-

D

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H

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V

L

F

L

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N

A

C

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A

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G

N

K

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V

L

L

R

R

L

F

L

L

P

P

P

P

L

L

C

I

L

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M

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E

E

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A

I

D

D

E

R

F

A

L

I

A

S

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V

F

L

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R

R

E

V

I

L

L

GT 96:97 (≠ GA 96:97) binding
K242 (= K237) modified: N6-(pyridoxal phosphate)lysine
T271 (= T264) binding

2eh6A Crystal structure of acetylornithine aminotransferase from aquifex aeolicus vf5
36% identity, 99% coverage: 3:373/373 of query aligns to 3:375/375 of 2eh6A

query
sites
2eh6A

L

L

F

M

D

N

V

N

Y

Y

P

A

L

R

Y

L

D

P

I

V

N

K

I

F

V

V

K

R

G

G

Q

K

G

G

C

V

K

Y

V

L

W

Y

D

D

E

E

N

E

G

G

T

K

E

E

Y

Y

L

L

D

D

L

F

Y

V

G

S

G

G

H

I

A

G

V

V

I

N

S

S

I

L

G

G

H

H

A

A

H

Y

P

P

H

K

Y

L

V

T

E

E

M

A

I

L

S

K

N

E

Q

Q

V

V

A

E

T

K

L

L

G

L

F

H

Y

V

S

S

N

N

S

L

V

Y

I

E

N

N

K

P

L

W

Q

Q

Q

E

Q

E

V

L

A

A

E

H

R

K

L

L

G

V

K

K

I

-

S

H

G

F

Y

W

E

T

D

E

Y

G

S

K

L

V

F

F

L

F

I

A

N

N

S
|
S

G
|
G

A
x
T

E

E

A

S

N

V

E

E

N

A

A

A

L

I

K

K

L

L

A

A

S

R

F

K

Y

Y

-

W

-

R

-

D

-

K

-

G

N

K

G

N

R

K

T

W

K

K

V

F

I

I

S

S

F

F

S

E

K

N

A

S

F
|
F

H
|
H

G
|
G

R

R

T

T

S

Y

L

G

A

S

V

L

E

S

A

A

T

T

N

G

N

Q

P

P

T

K

I

F

-

H

-

K

-

G

I

F

A

E

P

P

I

L

N

V

N

P

N

G

G

-

H

-

V

F

T

S

Y

Y

L

A

P

K

L

L

N

N

D

D

I

I

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D

A

S

M

V

K

Y

Q

K

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L

L

L

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-

D

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C

A

A

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V

I

I

I

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|
E

G

V

I

I

Q

Q

G

G

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E

G

G

G

G

I

V

K

N

I

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P

A

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S

T

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D

F

F

M

L

Q

S

E

K

L

L

R

Q

K

E

V

I

C

C

T

K

E

E

T

K

G

D

T

V

I

L

L

L

I

I

L

I

D
|
D

E

E

I
x
V

Q
|
Q

S

T

G

G

Y

I

G

G

R

R

S

T

G

G

K

E

F

F

F

Y

A

A

H

Y

Q

Q

Y

H

N

F

H

N

I

L

Q

K

P

P

D

D

I

V

I

I

T

A

V

L

A

A

K
|
K

G

G

I

L

G

G

N

G

G

G

F

V

P

P

M

I

A

G

G

A

V

I

L

L

-

A

-

R

-

E

-

E

I

V

S

A

P

Q

M

S

F

F

K

T

P

P

V

-

Y

-

G

G

Q

S

L

H

G

G

T
x
S

T
|
T

F
|
F

G

G

G

G

N

N

H

P

L

L

A

A

C

C

S

R

A

A

A

G

L

T

A

V

V

V

M

V

D

D

V

E

I

V

E

E

Q

K

D

-

N

-

L

L

V

L

E

P

N

H

A

V

K

R

A

E

V

V

G

G

D

N

Y

Y

L

F

L

K

E

E

E

K

L

L

K

K

K

E

F

L

P

G

Q

K

I

G

K

K

E

-

V

V

R

K

G

G

R

R

G

G

L

L

M

M

I

L

G

G

L

L

E

E

F

L

E

E

E

R

P

E

I

C

K

K

E

D

L

Y

R

V

S

L

R

K

L

A

I

L

Y

-

D

E

E

K

H

G

V

L

F

L

T

I

G

N

A

C

S

T

G

A

T

G

N

K

V

V

L

L

R
|
R

L

F

L

L

P

P

P

P

L

L

C

I

L

I

S

Q

M

K

E

E

E

H

A

I

D

D

E

R

F

A

L

I

A

S

R

V

F

L

K

R

R

E

V

I

L

L

active site: F127 (= F123), E179 (= E175), D212 (= D208), Q215 (= Q211), K241 (= K237), T270 (= T264), R352 (= R350)
binding pyridoxal-5'-phosphate: S94 (= S95), G95 (= G96), T96 (≠ A97), F127 (= F123), H128 (= H124), G129 (= G125), E179 (= E175), D212 (= D208), V214 (≠ I210), Q215 (= Q211), K241 (= K237), S269 (≠ T263), T270 (= T264), F271 (= F265)

P40732 Acetylornithine/succinyldiaminopimelate aminotransferase; ACOAT; DapATase; Succinyldiaminopimelate transferase; EC 2.6.1.11; EC 2.6.1.17 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
34% identity, 99% coverage: 3:373/373 of query aligns to 17:400/405 of P40732

query
sites
P40732

L

I

F

L

D

P

V

V

Y

Y

P

A

L

P

Y

A

D

D

I

F

N

I

I

P

V

V

K

K

G

G

Q

K

G

G

C

S

K

R

V

V

W

W

D

D

E

Q

N

Q

G

G

T

K

E

E

Y

Y

L

I

D

D

L

F

Y

A

G

G

G

G

H

I

A

A

V

V

I

T

S

A

I

L

G

G

H

H

A

C

H

H

P

P

H

A

Y

L

V

V

E

E

M

A

I

L

S

K

N

S

Q

Q

V

G

A

E

T

T

L

L

G

W

F

H

Y

T

S

S

N

N

S

V

V

F

I

T

N

N

K

-

L

-

Q

-

Q

-

Q

E

V

P

A

A

E

L

R

R

L

L

G

G

K

R

-

K

-

L

I

I

S

D

G

A

Y

T

E

F

D

A

Y

E

S

R

L

V

F

L

L

F

I

M

N

N

S

S

G
|
G

A
x
T

E

E

A

A

N

N

E

E

N

T

A

A

L

F

K

K

L

L

A

A

S

R

F

H

Y

Y

N

A

G

C

-

V

-

R

-

H

-

S

-

P

-

F

R

K

T

T

K

K

V

I

I

I

S

A

F

F

S

H

K

N

A

A

F

F

H

H

G

G

R

R

T

S

S

L

L

F

A

T

V

V

E

S

A

V

T

G

N

G

N

Q

P

P

T

K

I

Y

I

S

A

D

P

G

I

F

N

G

N

P

N

K

-

P

G

A

H

D

V

I

T

I

Y

H

L

V

P

P

L

F

N

N

D

D

I

L

E

H

A

A

M

V

K

K

Q

A

E

V

L

M

A

-

K

D

G

D

D

H

V

T

C

C

A

A

V

V

I

V

I

V

E

E

G

P

I

I

Q

Q

G

G

V

E

G

G

G

G

I

V

K

Q

I

A

P

A

T

T

T

P

E

E

F

F

M

L

Q

K

E

G

L

L

R

R

K

D

V

L

C

C

T

D

E

E

T

H

G

Q

T

A

I

L

L

L

I

V

L

F

D

D

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E

I

V

Q

Q

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C

G

G

Y

M

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G

R

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S

T

G

G

K

D

F

L

F

F

A

A

H

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Q

M

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H

N

Y

H

G

I

V

Q

T

P

P

D

D

I

I

I

L

T

T

V

S

A

A

K
|
K

G

A

I

L

G

G

N

G

G

G

F

F

P

P

M

V

A

S

G

A

V

M

L

L

I

T

S

T

P

Q

M

E

F

I

K

A

P

S

V

A

Y

F

-

H

-

V

G

G

Q

S

L

H

G

G

T

S

T
|
T

F

Y

G

G

G

G

N

N

H

P

L

L

A

A

C

C

S

A

A

V

A

A

L

G

A

A

V

A

M

F

D

D

V

I

I

I

E

N

Q

T

D

P

N

E

L

V

V

L

E

Q

N

G

A

I

K

H

A

T

V

K

G

R

D

Q

Y

Q

L

F

L

V

E

Q

E

H

L

L

K

Q

-

A

-

I

-

D

-

E

K

Q

F

F

P

D

Q

I

I

F

K

S

E

D

V

I

R

R

G

G

R

M

G

G

L

L

M

L

I

I

G

G

L

A

E

E

F

L

E

K

E

P

P

K

I

Y

K

K

E

G

L

R

R

A

S

R

R

D

L

F

I

L

Y

Y

-

A

-

G

-

A

D

E

E

A

H

G

V

V

F

M

T

V

G

L

A

N

S

A

G

G

T

A

N

D

V

V

L

M

R

R

L

F

L

A

P

P

P

S

L

L

C

V

L

V

S

E

M

E

E

A

E

D

A

I

D

H

E

E

F

G

L

M

A

Q

R

R

F

F

K

A

R

Q

V

A

L

V

GT 108:109 (≠ GA 96:97) binding
K255 (= K237) modified: N6-(pyridoxal phosphate)lysine
T284 (= T264) binding

4jevB N-acetylornithine aminotransferase from s. Typhimurium complexed with gabaculine
34% identity, 99% coverage: 3:373/373 of query aligns to 12:395/402 of 4jevB

query
sites
4jevB

L

I

F

L

D

P

V

V

Y

Y

P

A

L

P

Y

A

D

D

I

F

N

I

I

P

V

V

K

K

G

G

Q

K

G

G

C

S

K

R

V

V

W

W

D

D

E

Q

N

Q

G

G

T

K

E

E

Y

Y

L

I

D

D

L

F

Y

A

G

G

G

G

H
x
I

A

A

V

V

I

T

S

A

I

L

G

G

H

H

A

C

H

H

P

P

H

A

Y

L

V

V

E

E

M

A

I

L

S

K

N

S

Q

Q

V

G

A

E

T

T

L

L

G

W

F

H

Y

T

S

S

N

N

S

V

V

F

I

T

N

N

K

-

L

-

Q

-

Q

-

Q

E

V

P

A

A

E

L

R

R

L

L

G

G

K

R

-

K

-

L

I

I

S

D

G

A

Y

T

E

F

D

A

Y

E

S

R

L

V

F

L

L

F

I

M

N

N

S
|
S

G
|
G

A
x
T

E

E

A

A

N
|
N

E

E

N

T

A

A

L

F

K

K

L

L

A

A

S

R

F

H

Y

Y

N

A

G

C

-

V

-

R

-

H

-

S

-

P

-

F

R

K

T

T

K

K

V

I

I

I

S

A

F

F

S

H

K

N

A

A

F
|
F

H
|
H

G
|
G

R

R

T

S

S

L

L

F

A

T

V

V

E

S

A

V

T

G

N

G

N

Q

P

P

T

K

I

Y

I

S

A

D

P

G

I

F

N

G

N

P

N

K

-

P

G

A

H

D

V

I

T

I

Y

H

L

V

P

P

L

F

N

N

D

D

I

L

E

H

A

A

M

V

K

K

Q

A

E

V

L

M

A

-

K

D

G

D

D

H

V

T

C

C

A

A

V

V

I

V

I

V

E
|
E

G

P

I

I

Q

Q

G

G

V
x
E

G

G

G

G

I

V

K

Q

I

A

P

A

T

T

T

P

E

E

F

F

M

L

Q

K

E

G

L

L

R

R

K

D

V

L

C

C

T

D

E

E

T

H

G

Q

T

A

I

L

L

L

I

V

L

F

D
|
D

E

E

I
x
V

Q
|
Q

S

C

G

G

Y

M

G

G

R

R

S

T

G

G

K

D

F

L

F

F

A

A

H

Y

Q

M

Y

H

N

Y

H

G

I

V

Q

T

P

P

D

D

I

I

I

L

T

T

V

S

A

A

K
|
K

G

A

I

L

G

G

N

G

G

G

F

F

P

P

M

V

A

S

G

A

V

M

L

L

I

T

S

T

P

Q

M

E

F

I

K

A

P

S

V

A

Y

F

-

H

-

V

G

G

Q

S

L

H

G

G

T
x
S

T
|
T

F

Y

G

G

G

G

N

N

H

P

L

L

A

A

C

C

S

A

A

V

A

A

L

G

A

A

V

T

M

F

D

D

V

I

I

I

E

N

Q

T

D

P

N

E

L

V

V

L

E

Q

N

G

A

I

K

H

A

T

V

K

G

R

D

Q

Y

Q

L

F

L

V

E

Q

E

H

L

L

K

Q

-

A

-

I

-

D

-

E

K

Q

F

F

P

D

Q

I

I

F

K

S

E

D

V

I

R

R

G

G

R

M

G

G

L

L

M

L

I

I

G

G

L

A

E

E

F

L

E

K

E

P

P

K

I

Y

K

K

E

G

L

R

R

A

S

R

R

D

L

F

I

L

Y

Y

-

A

-

G

-

A

D

E

E

A

H

G

V

V

F

M

T

V

G

L

A

N

S

A

G

G

T

A

N

D

V

V

L

M

R
|
R

L

F

L

A

P

P

P

S

L

L

C

V

L

V

S

E

M

E

E

A

E

D

A

I

D

H

E

E

F

G

L

M

A

Q

R

R

F

F

K

A

R

Q

V

A

L

V

active site: F136 (= F123), E188 (= E175), D221 (= D208), Q224 (= Q211), K250 (= K237), T279 (= T264), R372 (= R350)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: I46 (≠ H37), S102 (= S95), G103 (= G96), T104 (≠ A97), N107 (= N100), F136 (= F123), H137 (= H124), G138 (= G125), E188 (= E175), E193 (≠ V180), D221 (= D208), V223 (≠ I210), Q224 (= Q211), K250 (= K237), S278 (≠ T263), T279 (= T264), R372 (= R350)

4jewA N-acetylornithine aminotransferase from s. Typhimurium complexed with l-canaline
34% identity, 99% coverage: 3:373/373 of query aligns to 12:390/397 of 4jewA

query
sites
4jewA

L

I

F

L

D

P

V

V

Y

Y

P

A

L

P

Y

A

D

D

I

F

N

I

I

P

V

V

K
|
K

G

G

Q
x
K

G

G

C

S

K

R

V

V

W
|
W

D

D

E

Q

N

Q

G
|
G

T

K

E
|
E

Y

Y

L

I

D

D

L

F

Y

A

G

G

G

G

H
x
I

A

A

V

V

I

T

S

A

I

L

G

G

H

H

A

C

H

H

P

P

H

A

Y

L

V

V

E

E

M

A

I

L

S

K

N

S

Q

Q

V

G

A

E

T

T

L

L

G

W

F

H

Y

T

S

S

N

N

S

V

V

F

I

T

N

N

K

-

L

-

Q

-

Q

-

Q

E

V

P

A

A

E

L

R

R

L

L

G

G

K

R

-

K

-

L

I

I

S

D

G

A

Y

T

E

F

D

A

Y

E

S

R

L

V

F

L

L

F

I

M

N

N

S
|
S

G
|
G

A
x
T

E

E

A

A

N
|
N

E

E

N

T

A

A

L

F

K

K

L

L

A

A

S

R

F

H

Y

Y

N

A

G

C

-

V

-

R

-

H

-

S

-

P

-

F

R

K

T

T

K

K

V

I

I

I

S

A

F

F

S

H

K

N

A

A

F
|
F

H
|
H

G
|
G

R
|
R

T

S

S

L

L

F

A

T

V

V

E

S

A

V

T

G

N

G

N

Q

P

P

T

K

I

Y

I

S

A

D

P

G

I

F

N

G

N

P

N

K

-

P

G

A

H

D

V

I

T

I

Y

H

L

V

P

P

L

F

N

N

D

D

I

L

E

H

A

A

M

V

K

K

Q

A

E

V

L

M

A

-

K

D

G

D

D

H

V

T

C

C

A

A

V

V

I

V

I

V

E
|
E

G

P

I

I

Q

Q

G

G

V
x
E

G

G

G

G

I

V

K

Q

I

A

P

A

T

T

T

P

E

E

F

F

M

L

Q

K

E

G

L

L

R

R

K

D

V

L

C

C

T

D

E

E

T

H

G

Q

T

A

I

L

L

L

I

V

L

F

D
|
D

E

E

I
x
V

Q
|
Q

S

C

G

G

Y

M

G

G

R

R

S

T

G

G

K

D

F

L

F

F

A

A

H

Y

Q

M

Y

H

N

Y

H

G

I

V

Q

T

P

P

D

D

I

I

I

L

T

T

V

S

A

A

K
|
K

G

A

I

L

G

G

N

G

G

G

F

F

P

P

M

V

A

S

G

A

V

M

L

L

I

T

S

T

P

-

M

-

F

-

K

Q

P

E

V

I

Y

A

G

S

Q

A

L

F

G

G

T

S

T
|
T

F

Y

G

G

G

G

N

N

H

P

L

L

A

A

C

C

S

A

A

V

A

A

L

G

A

A

V

T

M

F

D

D

V

I

I

I

E

N

Q

T

D

P

N

E

L

V

V

L

E

Q

N

G

A

I

K

H

A

T

V

K

G

R

D

Q

Y

Q

L

F

L

V

E

Q

E

H

L

L

K

Q

-

A

-

I

-

D

-

E

K

Q

F

F

P

D

Q

I

I

F

K

S

E

D

V

I

R

R

G

G

R

M

G

G

L

L

M

L

I

I

G

G

L

A

E

E

F

L

E

K

E

P

P

K

I

Y

K

K

E

G

L

R

R

A

S

R

R

D

L

F

I

L

Y

Y

-

A

-

G

-

A

D

E

E

A

H

G

V

V

F

M

T

V

G

L

A

N

S

A

G

G

T

A

N

D

V

V

L

M

R
|
R

L

F

L

A

P

P

P

S

L

L

C

V

L

V

S

E

M

E

E

A

E

D

A

I

D

H

E

E

F

G

L

M

A

Q

R

R

F

F

K

A

R

Q

V

A

L

V

active site: F136 (= F123), E188 (= E175), D221 (= D208), Q224 (= Q211), K250 (= K237), T274 (= T264), R367 (= R350)
binding (2S)-2-azanyl-4-[(E)-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]oxy-butanoic acid: I46 (≠ H37), S102 (= S95), G103 (= G96), T104 (≠ A97), N107 (= N100), F136 (= F123), H137 (= H124), G138 (= G125), R139 (= R126), E188 (= E175), E193 (≠ V180), D221 (= D208), V223 (≠ I210), Q224 (= Q211), K250 (= K237)
binding picric acid: K25 (= K16), K27 (≠ Q18), W32 (= W23), G36 (= G27), E38 (= E29)

2pb0A Structure of biosynthetic n-acetylornithine aminotransferase from salmonella typhimurium: studies on substrate specificity and inhibitor binding (see paper)
34% identity, 99% coverage: 3:373/373 of query aligns to 6:384/389 of 2pb0A

query
sites
2pb0A

L

I

F

L

D

P

V

V

Y

Y

P

A

L

P

Y

A

D

D

I

F

N

I

I

P

V

V

K

K

G

G

Q

K

G

G

C

S

K

R

V

V

W

W

D

D

E

Q

N

Q

G

G

T

K

E

E

Y

Y

L

I

D

D

L

F

Y

A

G

G

G

G

H

I

A

A

V

V

I

T

S

A

I

L

G

G

H

H

A

C

H

H

P

P

H

A

Y

L

V

V

E

E

M

A

I

L

S

K

N

S

Q

Q

V

G

A

E

T

T

L

L

G

W

F

H

Y

T

S

S

N

N

S

V

V

F

I

T

N

N

K

-

L

-

Q

-

Q

-

Q

E

V

P

A

A

E

L

R

R

L

L

G

G

K

R

-

K

-

L

I

I

S

D

G

A

Y

T

E

F

D

A

Y

E

S

R

L

V

F

L

L

F

I

M

N

N

S
|
S

G
|
G

A
x
T

E

E

A

A

N
|
N

E

E

N

T

A

A

L

F

K

K

L

L

A

A

S

R

F

H

Y

Y

N

A

G

C

-

V

-

R

-

H

-

S

-

P

-

F

R

K

T

T

K

K

V

I

I

I

S

A

F

F

S

H

K

N

A

A

F
|
F

H
|
H

G
|
G

R

R

T

S

S

L

L

F

A

T

V

V

E

S

A

V

T

G

N

G

N

Q

P

P

T

K

I

Y

I

S

A

D

P

G

I

F

N

G

N

P

N

K

-

P

G

A

H

D

V

I

T

I

Y

H

L

V

P

P

L

F

N

N

D

D

I

L

E

H

A

A

M

V

K

K

Q

A

E

V

L

M

A

-

K

D

G

D

D

H

V

T

C

C

A

A

V

V

I

V

I

V

E
|
E

G

P

I

I

Q

Q

G

G

V

E

G

G

G

G

I

V

K

Q

I

A

P

A

T

T

T

P

E

E

F

F

M

L

Q

K

E

G

L

L

R

R

K

D

V

L

C

C

T

D

E

E

T

H

G

Q

T

A

I

L

L

L

I

V

L

F

D
|
D

E

E

I
x
V

Q
|
Q

S

C

G

G

Y

M

G

G

R

R

S

T

G

G

K

D

F

L

F

F

A

A

H

Y

Q

M

Y

H

N

Y

H

G

I

V

Q

T

P