SitesBLAST
Comparing 353418 FitnessBrowser__Btheta:353418 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3uyyB Crystal structures of branched-chain aminotransferase from deinococcus radiodurans complexes with alpha-ketoisocaproate and l-glutamate suggest its radio-resistance for catalysis (see paper)
47% identity, 99% coverage: 3:338/339 of query aligns to 2:337/337 of 3uyyB
- active site: K181 (= K183)
- binding pyridoxal-5'-phosphate: R79 (= R80), R171 (= R173), K181 (= K183), Y186 (= Y188), E217 (= E219), G219 (= G221), A220 (= A222), A221 (= A223), S244 (= S244), I245 (= I245), T246 (= T246), G282 (= G281), T283 (= T282)
3uzoA Crystal structures of branched-chain aminotransferase from deinococcus radiodurans complexes with alpha-ketoisocaproate and l-glutamate suggest its radio-resistance for catalysis (see paper)
48% identity, 99% coverage: 4:338/339 of query aligns to 1:335/335 of 3uzoA
- active site: K179 (= K183)
- binding glutamic acid: Y48 (= Y51), F53 (= F56), R122 (= R125), V134 (= V137), Y152 (= Y155), T281 (= T282), A282 (= A283)
- binding pyridoxal-5'-phosphate: R77 (= R80), K179 (= K183), Y184 (= Y188), E215 (= E219), G217 (= G221), A218 (= A222), A219 (= A223), N220 (= N224), L240 (= L242), S242 (= S244), I243 (= I245), T244 (= T246), G280 (= G281), T281 (= T282)
3uzbA Crystal structures of branched-chain aminotransferase from deinococcus radiodurans complexes with alpha-ketoisocaproate and l-glutamate suggest its radio-resistance for catalysis (see paper)
48% identity, 99% coverage: 4:338/339 of query aligns to 1:335/335 of 3uzbA
- active site: K179 (= K183)
- binding 2-oxo-4-methylpentanoic acid: Y120 (= Y123), K179 (= K183), T281 (= T282), A282 (= A283)
- binding pyridoxal-5'-phosphate: R77 (= R80), K179 (= K183), Y184 (= Y188), E215 (= E219), G217 (= G221), A218 (= A222), A219 (= A223), N220 (= N224), S242 (= S244), I243 (= I245), T244 (= T246), G280 (= G281), T281 (= T282)
6jifB Crystal structures of branched-chain aminotransferase from pseudomonas sp. Uw4 (see paper)
47% identity, 99% coverage: 4:338/339 of query aligns to 1:334/334 of 6jifB
- binding pyridoxal-5'-phosphate: R77 (= R80), R169 (= R173), K179 (= K183), Y184 (= Y188), E215 (= E219), S218 (≠ A222), A219 (= A223), N220 (= N224), L239 (= L242), G241 (≠ S244), I242 (= I245), T243 (= T246), G279 (= G281), T280 (= T282)
A0R066 Branched-chain-amino-acid aminotransferase; BCAT; EC 2.6.1.42 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see 2 papers)
41% identity, 99% coverage: 1:335/339 of query aligns to 21:364/368 of A0R066
- R101 (= R80) binding
- Y209 (= Y188) binding
- K299 (≠ T273) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- T314 (= T282) binding
3dtgA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design
41% identity, 99% coverage: 1:335/339 of query aligns to 16:359/363 of 3dtgA
- active site: K199 (= K183)
- binding O-benzylhydroxylamine: F72 (= F56), Y171 (= Y155), Y204 (= Y188)
- binding pyridoxal-5'-phosphate: R96 (= R80), R189 (= R173), K199 (= K183), Y204 (= Y188), E235 (= E219), G238 (≠ A222), L263 (= L242), I266 (= I245), T267 (= T246), T309 (= T282)
3dtfA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design (see paper)
41% identity, 99% coverage: 1:335/339 of query aligns to 16:359/363 of 3dtfA
3ht5A Crystal structure of ilve a branched chain amino acid transaminase from mycobacterium tuberculosis (see paper)
40% identity, 95% coverage: 16:336/339 of query aligns to 3:332/335 of 3ht5A
- active site: K171 (= K183)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R68 (= R80), K171 (= K183), Y176 (= Y188), E207 (= E219), G210 (≠ A222), M211 (≠ A223), N212 (= N224), L235 (= L242), G237 (≠ S244), I238 (= I245), T239 (= T246), T281 (= T282)
5u3fA Structure of mycobacterium tuberculosis ilve, a branched-chain amino acid transaminase, in complex with d-cycloserine derivative (see paper)
38% identity, 95% coverage: 16:336/339 of query aligns to 2:323/326 of 5u3fA
- active site: K162 (= K183)
- binding (5-hydroxy-6-methyl-4-{[(3-oxo-2,3-dihydro-1,2-oxazol-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: G45 (= G58), R67 (= R80), Y110 (= Y123), K162 (= K183), Y167 (= Y188), E198 (= E219), G201 (≠ A222), M202 (≠ A223), N203 (= N224), L226 (= L242), G228 (≠ S244), I229 (= I245), T230 (= T246), T272 (= T282)
7nwmA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 12 (see paper)
33% identity, 95% coverage: 8:328/339 of query aligns to 25:356/365 of 7nwmA
- binding pyridoxal-5'-phosphate: R98 (= R80), R191 (= R173), K201 (= K183), Y206 (= Y188), E236 (= E219), T239 (≠ A222), M240 (≠ A223), L265 (= L242), G267 (≠ S244), V268 (≠ I245), T269 (= T246), G311 (= G281), T312 (= T282)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-methoxy-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F11), Y69 (= Y51), F74 (= F56), Y140 (= Y123), G153 (= G136), V154 (= V137), Y172 (= Y155), Q213 (≠ N195), Q223 (≠ C205), T239 (≠ A222), A313 (= A283)
7nweA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 10 (see paper)
33% identity, 95% coverage: 8:328/339 of query aligns to 25:356/365 of 7nweA
- binding pyridoxal-5'-phosphate: R98 (= R80), K201 (= K183), Y206 (= Y188), E236 (= E219), T239 (≠ A222), M240 (≠ A223), L265 (= L242), G267 (≠ S244), V268 (≠ I245), T269 (= T246), G311 (= G281), T312 (= T282)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-methyl-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F11), Y69 (= Y51), F74 (= F56), Y140 (= Y123), G153 (= G136), V154 (= V137), Y172 (= Y155), Q213 (≠ N195), Q223 (≠ C205), T239 (≠ A222), A313 (= A283)
7nwcA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 2 (see paper)
33% identity, 95% coverage: 8:328/339 of query aligns to 25:356/365 of 7nwcA
- binding pyridoxal-5'-phosphate: R98 (= R80), R191 (= R173), K201 (= K183), Y206 (= Y188), E236 (= E219), T239 (≠ A222), M240 (≠ A223), N241 (= N224), L265 (= L242), G267 (≠ S244), V268 (≠ I245), T269 (= T246), G311 (= G281), T312 (= T282)
- binding 3-(5-chloranyl-2,4-dimethoxy-phenyl)-6-(trifluoromethyl)-1H-pyrimidine-2,4-dione: F28 (= F11), Y172 (= Y155), Q223 (≠ C205), T239 (≠ A222), A313 (= A283)
7nwbA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 1 (see paper)
33% identity, 95% coverage: 8:328/339 of query aligns to 25:356/365 of 7nwbA
- binding pyridoxal-5'-phosphate: R98 (= R80), R191 (= R173), K201 (= K183), Y206 (= Y188), E236 (= E219), T239 (≠ A222), M240 (≠ A223), L265 (= L242), G267 (≠ S244), V268 (≠ I245), T269 (= T246), G311 (= G281), T312 (= T282)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-fluoranyl-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F11), Y69 (= Y51), F74 (= F56), Y140 (= Y123), G153 (= G136), V154 (= V137), Y172 (= Y155), Q213 (≠ N195), Q223 (≠ C205), A313 (= A283)
7nwaA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and compound a (see paper)
33% identity, 95% coverage: 8:328/339 of query aligns to 25:356/365 of 7nwaA
- binding pyridoxal-5'-phosphate: R98 (= R80), K201 (= K183), Y206 (= Y188), E236 (= E219), T239 (≠ A222), N241 (= N224), L265 (= L242), G267 (≠ S244), V268 (≠ I245), T269 (= T246), T312 (= T282)
- binding 2-[[4-chloranyl-2,6-bis(fluoranyl)phenyl]methylamino]-7-oxidanylidene-5-propyl-4H-pyrazolo[1,5-a]pyrimidine-3-carbonitrile: R142 (= R125), G153 (= G136), V154 (= V137), Y172 (= Y155), F173 (= F156), V181 (≠ Y165), Q223 (≠ C205), V237 (≠ C220), T239 (≠ A222), M240 (≠ A223), G311 (= G281), A313 (= A283), C317 (≠ S287)
7nyaA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor bay-069 (compound 36) (see paper)
33% identity, 95% coverage: 8:328/339 of query aligns to 24:355/364 of 7nyaA
- binding pyridoxal-5'-phosphate: R97 (= R80), R190 (= R173), K200 (= K183), Y205 (= Y188), E235 (= E219), T238 (≠ A222), M239 (≠ A223), L264 (= L242), G266 (≠ S244), V267 (≠ I245), T268 (= T246), G310 (= G281), T311 (= T282)
- binding 3-[4-chloranyl-3-(2-methylphenoxy)naphthalen-1-yl]-6-(trifluoromethyl)-5~{H}-pyrimidine-2,4-dione: F27 (= F11), Y68 (= Y51), F73 (= F56), Y139 (= Y123), G152 (= G136), V153 (= V137), Y171 (= Y155), Q212 (≠ N195), Q222 (≠ C205), T238 (≠ A222), A312 (= A283)
7ny9A Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 38 (see paper)
33% identity, 95% coverage: 8:328/339 of query aligns to 24:355/364 of 7ny9A
- binding pyridoxal-5'-phosphate: R97 (= R80), R190 (= R173), K200 (= K183), Y205 (= Y188), E235 (= E219), T238 (≠ A222), M239 (≠ A223), N240 (= N224), L264 (= L242), G266 (≠ S244), V267 (≠ I245), T268 (= T246), T311 (= T282)
- binding 3-[2,6-bis(oxidanylidene)-4-(trifluoromethyl)-5~{H}-pyrimidin-1-yl]-~{N}-methyl-2-pyridin-2-yl-imidazo[1,2-a]pyridine-7-carboxamide: F27 (= F11), Y139 (= Y123), G152 (= G136), V153 (= V137), Y171 (= Y155), Q212 (≠ N195), Q221 (≠ S204), Q222 (≠ C205), V223 (≠ E206), T238 (≠ A222)
7ny2A Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 35 (see paper)
33% identity, 95% coverage: 8:328/339 of query aligns to 24:355/364 of 7ny2A
- binding pyridoxal-5'-phosphate: R97 (= R80), K200 (= K183), Y205 (= Y188), E235 (= E219), T238 (≠ A222), M239 (≠ A223), L264 (= L242), G266 (≠ S244), V267 (≠ I245), T268 (= T246), T311 (= T282)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-2-(2-methylphenoxy)naphthalene-1-carbonitrile: F27 (= F11), Y139 (= Y123), G152 (= G136), V153 (= V137), Y171 (= Y155), Q212 (≠ N195), Q222 (≠ C205), A312 (= A283)
2abjA Crystal structure of human branched chain amino acid transaminase in a complex with an inhibitor, c16h10n2o4f3scl, and pyridoxal 5' phosphate. (see paper)
33% identity, 95% coverage: 8:328/339 of query aligns to 26:357/366 of 2abjA
- active site: K202 (= K183)
- binding n'-(5-chlorobenzofuran-2-carbonyl)-2-(trifluoromethyl)benzenesulfonohydrazide: F29 (= F11), F75 (= F56), Y141 (= Y123), Y173 (= Y155), K202 (= K183), Q224 (≠ C205), T240 (≠ A222), M241 (≠ A223), G312 (= G281), A314 (= A283), C315 (≠ A284)
- binding pyridoxal-5'-phosphate: R99 (= R80), R192 (= R173), K202 (= K183), Y207 (= Y188), E237 (= E219), T240 (≠ A222), M241 (≠ A223), N242 (= N224), L266 (= L242), G268 (≠ S244), V269 (≠ I245), T270 (= T246), T313 (= T282)
7nxnA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 21(5-f) (see paper)
33% identity, 95% coverage: 8:328/339 of query aligns to 25:353/362 of 7nxnA
- binding pyridoxal-5'-phosphate: R98 (= R80), R188 (= R173), K198 (= K183), Y203 (= Y188), E233 (= E219), T236 (≠ A222), M237 (≠ A223), N238 (= N224), L262 (= L242), G264 (≠ S244), V265 (≠ I245), T266 (= T246), T309 (= T282)
- binding 4-[6-[bis(fluoranyl)-phenyl-methyl]-2,4-bis(oxidanylidene)-1~{H}-pyrimidin-3-yl]-5-fluoranyl-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F11), Y69 (= Y51), F74 (= F56), G153 (= G136), V154 (= V137), Y172 (= Y155), Q210 (≠ N195), Q219 (≠ S204), Q220 (≠ C205), T236 (≠ A222)
7ntrA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and substrate mimic 3-phenylpropionate (see paper)
33% identity, 95% coverage: 8:328/339 of query aligns to 25:353/362 of 7ntrA
- binding hydrocinnamic acid: F28 (= F11), Y69 (= Y51), V154 (= V137), T236 (≠ A222), G308 (= G281), T309 (= T282), A310 (= A283)
- binding pyridoxal-5'-phosphate: R98 (= R80), R188 (= R173), K198 (= K183), Y203 (= Y188), E233 (= E219), T236 (≠ A222), M237 (≠ A223), L262 (= L242), G264 (≠ S244), V265 (≠ I245), T266 (= T246), G308 (= G281), T309 (= T282)
Query Sequence
>353418 FitnessBrowser__Btheta:353418
MKEIDWANLSFGYMKTDYNVRINFRNGAWGELEVSSDEHLNLHMAATCLHYGQEAFEGLK
AFRGKDGKVRIFRLEENAARLQSTCQGILMAELPTERFKEAILKVVKLNERFIPPYETGA
SLYIRPLLIGTSAQVGVHPAEEYMFVVFVTPVGPYFKGGFSTNPYVIIREFDRAAPHGTG
IYKVGGNYAASLRANKKAHDLGYSCEFYLDAKEKKYIDECGAANFFGIKDNTYITPKSSS
ILPSITNKSLMQLAEDMGIKVERRPIPEEELETFEEAGACGTAAVISPIQRIDDLENGKS
YVISKDGKPGPICTKLYNKLRGIQYGDEPDTHGWVTIVE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory