SitesBLAST

F136 (≠ K56) mutation to A: Decreases association with PtsG EIIB domain.
H247 (= H168) binding
C257 (= C178) binding ; mutation to A: Strongly reduced activity; when associated with A-259.; mutation to S: Strongly reduced activity; when associated with S-259.
C259 (= C180) binding ; mutation to A: Strongly reduced activity; when associated with A-257.; mutation to S: Strongly reduced activity; when associated with S-257.
C264 (= C185) binding
R306 (≠ Q226) mutation to G: Forms dimers but not tetramers; when associated with G-310.
L310 (≠ I230) mutation to G: Forms dimers but not tetramers; when associated with G-306.

Sites not aligning to the query:

52 R→H: Shows increased expression and forms larger colonies.
86 H→R: Can be bound and inactivated by MtfA.

1z6rA Crystal structure of mlc from escherichia coli (see paper)
25% identity, 84% coverage: 44:308/317 of query aligns to 100:364/382 of 1z6rA

query
sites
1z6rA

V

L

I

L

G

D

Q

R

L

I

V

I

K

S

A

H

V

I

N

D

E

Q

V

F

K

F

T

I

F

R

A

H

E

Q

A

K

K

K

R

L

Y

E

T

R

I

L

A

T

G

S

I

I

G

A

I

I

G

T

T

L

P

P

G

G

I

I

V

I

D

D

C

T

T

E

N

N

R

G

I

I

V

V

L

-

G

-

A

-

E

H

N

R

I

M

Q

P

G

F

W

Y

E

E

N

D

L

V

K

K

-

E

-

M

-

P

L

L

A

G

D

E

R

A

M

L

E

E

K

Q

E

H

T

T

G

G

L

V

P

P

T

V

Q

Y

L

I

G

Q

N

H

D

D

A

I

N

S

L

A

M

W

G

T

L

M

G

A

E

E

T

A

M

L

Y

F

G

G

A

A

G

S

N

R

G

G

A

A

T

R

H

D

V

V

V

I

F

Q

L

V

T

V

V

I

G

D

T

H

G

N

I

V

G

G

G

A

A

G

V

V

I

I

I

T

D

D

G

G

K

H

L

L

F

L

N

H

G

A

Y

G

A

S

N

S

R

S

G

L

T

V

E

E

L

I

G

G

H
|
H

V

T

P

Q

L

V

I

D

A

P

N

Y

G

G

E

K

P

R

C
|
C

A

Y

C
|
C

G

G

S

N

I

H

G

G

C
|
C

L

L

E

E

H

T

Y

I

A

A

S

S

T

V

A

D

A

S

L

I

V

L

R

E

R

L

F

A

S

Q

K

L

R

R

I

L

A

N

E

Q

A

S

G

M

I

S

S

S

Y

M

P

L

N

H

-

G

E

Q

E

P

I

L

N

T

G

V

E

D

L

S

I

L

V

C

R

Q

L

A

Y

A

K

L

Q

R

G

G

D

D

K

L

I

L

A

A

A

K

E

D

S

I

L

I

N

T

E

G

H

V

C

G

D

A

F

H

L

V

G

G

H

R

G

I

I

L

A

A

G

I

F

M

I

V

N

N

I

L

F

F

S

N

P

P

Q

Q

R

K

V

I

V

L

I

I

G

G

G

S

G

P

L

L

S

S

E

K

A

A

G

A

D

D

F

I

Y

L

I

F

Q

P

K

V

V

I

S

S

E

D

K

S

A

I

L

R

R

Q

Y

Q

A

A

I

L

P

P

D

A

C

Y

A

S

V

Q

N

H

T

I

E

S

I

V

M

E

A

S

A

T

S

Q

L

F

G

S

N

N

K

Q

A

G

G

T

S

M

I

A

G

G

A

A

binding zinc ion: H223 (= H168), C233 (= C178), C235 (= C180), C240 (= C185)

7p7wBBB Ubiquitin-like protein SMT3,N-acetyl-D-glucosamine kinase
28% identity, 97% coverage: 8:313/317 of query aligns to 8:305/306 of 7p7wBBB

query
sites
7p7wBBB

G

G

I

F

D

D

L

I

G

G

T

T

S

K

V

I

K

E

Y

F

A

G

L

A

I

F

D

D

N

A

N

D

G

L

V

V

F

R

H

V

F

A

Q

R

G

E

K

R

L

V

P

A

S

T

N

P

A

T

D

E

V

-

S

S

A

Y

E

A

A

A

V

F

I

L

G

D

Q

A

L

I

V

V

K

T

A

L

V

V

N

N

E

N

V

-

K

-

T

-

F

-

A

A

E

D

A

A

K

E

R

F

Y

G

T

V

I

K

A

G

G

T

I

V

G

G

I

I

G

G

T

I

P

P

G

G

I

I

V

A

D

D

C

V

T

E

N

T

R

G

I

K

V

L

L

L

G

-

A

T

E

S

N

N

I

I

Q

P

G

A

W

A

E

M

N

G

L

H

K

T

L

L

A

Q

D

R

R

D

M

L

E

E

K

E

E

R

T

L

G

Q

L

R

P

P

T

V

Q

K

L

I

G

E

N

N

D

D

A

A

N

N

L

C

M

F

G

A

L

L

G

S

E

E

T

A

M

W

Y

D

G

E

A

D

G

L

N

R

G

G

A

E

T

P

H

S

V

V

V

L

F

G

L

L

T

I

V

L

G
|
G

T
|
T

G

G

I

V

G

G

A

G

V

L

I

I

I

F

D

N

G

G

K

K

L

V

F

H

N

S

G

G

Y

R

A

A

N

N

R

I

G

A

T

G

E

E

L

I

G

G

H
|
H

V

T

P

R

L

L

I

P

A

Y

N

D

G

A

E

L

-

K

-

L

-

G

-

M

-

E

-

N

-

A

-

P

-

I

-

F

P

P

C
|
C

A

G

C
|
C

G

K

S

N

I

S

G

G

C
|
C

L

I

E

D

H

N

Y

Y

A

L

S

S

T
x
G

A

R

A

G

L

F

V
x
E

R

Q

R

L

F

Y

S

D

K

H

R

-

I

-

A

-

E

-

A

-

G

-

I

-

S

-

Y

Y

P

F

N

S

E

E

E

K

I

L

N

S

G

A

E

P

L

E

I

I

V

I

R

A

L

H

Y

Y

K

E

Q

Q

G

G

D

E

K

R

I

R

A

A

A

V

E

Q

S

H

L

V

N

E

E

R

H

F

C

M

D

E

F

L

L

L

G

A

H

I

G

C

I

L

A

A

G

N

F

I

I

F

N

T

I

C

F

L

S

D

P

P

Q

H

R

V

V

V

I

L

G

G

G
|
G

L

L

S

S

E
x
N

A

F

G

E

D

L

F

I

Y

Y

I

-

Q

Q

K

E

V

L

S

P

E

-

K

K

A

R

L

L

R

P

Y

A

A

H

I

L

P

L

D

H

C

V

A

A

V

K

N

L

T

P

E

K

I

I

M

I

A

K

A

A

S

R

L

H

G

G

N

D

K

A

A

G

G

G

S

V

I

R

G

G

A

A

S

-

L

-

F

F

L

L

N

N

binding adenosine-5'-diphosphate: G135 (= G142), T136 (= T143), G196 (≠ T192), E200 (≠ V196), G258 (= G262), G259 (= G263), N262 (≠ E266)
binding zinc ion: H161 (= H168), C182 (= C178), C184 (= C180), C189 (= C185)

7p9lAAA Ubiquitin-like protein SMT3,N-acetyl-D-glucosamine kinase
28% identity, 97% coverage: 8:313/317 of query aligns to 5:302/303 of 7p9lAAA

query
sites
7p9lAAA

G

G

I

F

D

D

L

I

G

G

T

T

S

K

V

I

K

E

Y

F

A

G

L

A

I

F

D

D

N

A

N

D

G

L

V

V

F

R

H

V

F

A

Q

R

G

E

K

R

L

V

P

A

S

T

N

P

A

T

D

E

V

-

S

S

A

Y

E

A

A

A

V

F

I

L

G

D

Q

A

L

I

V

V

K

T

A

L

V

V

N

N

E

N

V

-

K

-

T

-

F

-

A

A

E

D

A

A

K

E

R

F

Y

G

T

V

I

K

A

G

G

T

I

V

G

G

I

I

G

G

T

I

P
|
P

G
|
G

I

I

V

A

D

D

C

V

T

E

N

T

R

G

I

K

V

L

L

L

G

-

A

T

E
x
S

N

N

I

I

Q

P

G

A

W

A

E

M

N

G

L

H

K

T

L

L

A

Q

D

R

R

D

M

L

E

E

K

E

E

R

T

L

G

Q

L

R

P

P

T

V

Q

K

L

I

G

E

N
|
N

D
|
D

A

A

N

N

L

C

M

F

G

A

L

L

G

S

E

E

T

A

M

W

Y

D

G

E

A

D

G

L

N

R

G

G

A

E

T

P

H

S

V

V

V

L

F

G

L

L

T

I

V

L

G
|
G

T
|
T

G
|
G

I
x
V

G
|
G

G

G

A

G

V

L

I

I

I

F

D

N

G

G

K

K

L

V

F

H

N

S

G

G

Y

R

A

A

N

N

R

I

G

A

T

G

E
|
E

L

I

G

G

H
|
H

V

T

P

R

L

L

I

P

A

Y

N

D

G

A

E

L

-

K

-

L

-

G

-

M

-

E

-

N

-

A

-

P

-

I

-

F

P

P

C
|
C

A

G

C
|
C

G

K

S

N

I

S

G

G

C
|
C

L

I

E
x
D

H

N

Y

Y

A

L

S

S

T

G

A

R

A

G

L

F

V

E

R

Q

R

L

F

Y

S

D

K

H

R

-

I

-

A

-

E

-

A

-

G

-

I

-

S

-

Y

Y

P

F

N

S

E

E

E

K

I

L

N

S

G

A

E

P

L
x
E

I

I

V

I

R

A

L
x
H

Y

Y

K

E

Q

Q

G

G

D

E

K

R

I

R

A

A

A

V

E

Q

S

H

L

V

N

E

E

R

H

F

C

M

D

E

F

L

L

L

G

A

H

I

G

C

I

L

A

A

G

N

F

I

I

F

N

T

I

C

F

L

S

D

P

P

Q

H

R

V

V

V

I

L

G

G

L

L

S

S

E

N

A

F

G

E

D

L

F

I

Y

Y

I

-

Q

Q

K

E

V

L

S

P

E

-

K

K

A

R

L

L

R

P

Y

A

A

H

I

L

P

L

D

H

C

V

A

A

V

K

N

L

T

P

E

K

I

I

M

I

A

K

A

A

S

R

L

H

G

G

N

D

K

A

A

G

G

G

S

V

I

R

G

G

A

A

S

-

L

-

F

F

L

L

N

N

binding 2-acetamido-2-deoxy-6-O-phosphono-beta-D-glucopyranose: P66 (= P74), G67 (= G75), S79 (≠ E89), N105 (= N115), D106 (= D116), G132 (= G142), T133 (= T143), G134 (= G144), V135 (≠ I145), G136 (= G146), E155 (= E165), H158 (= H168), D188 (≠ E187)
binding zinc ion: H158 (= H168), C179 (= C178), C181 (= C180), C186 (= C185), E212 (≠ L219), H216 (≠ L223)

7p9pAAA Ubiquitin-like protein SMT3,N-acetyl-D-glucosamine kinase
28% identity, 97% coverage: 8:313/317 of query aligns to 6:303/304 of 7p9pAAA

query
sites
7p9pAAA

G

G

I

F

D

D

L

I

G

G

G
|
G

T
|
T

S
x
K

V

I

K

E

Y

F

A

G

L

A

I

F

D

D

N

A

N

D

G

L

V

V

F

R

H

V

F

A

Q

R

G

E

K

R

L

V

P

A

S

T

N

P

A

T

D

E

V

-

S

S

A

Y

E

A

A

A

V

F

I

L

G

D

Q

A

L

I

V

V

K

T

A

L

V

V

N

N

E

N

V

-

K

-

T

-

F

-

A

A

E

D

A

A

K

E

R

F

Y

G

T

V

I

K

A

G

G

T

I

V

G

G

I

I

G

G

T

I

P

P

G

G

I

I

V

A

D

D

C

V

T

E

N

T

R

G

I

K

V

L

L

L

G

-

A

T

E

S

N

N

I

I

Q

P

G

A

W

A

E

M

N

G

L

H

K

T

L

L

A

Q

D

R

R

D

M

L

E

E

K

E

E

R

T

L

G

Q

L

R

P

P

T

V

Q

K

L

I

G

E

N

N

D

D

A

A

N

N

L

C

M

F

G

A

L

L

G

S

E

E

T

A

M

W

Y

D

G

E

A

D

G

L

N

R

G

G

A

E

T

P

H

S

V

V

V

L

F

G

L

L

T

I

V

L

G
|
G

T
|
T

G

G

I

V

G

G

A

G

V

L

I

I

I

F

D

N

G

G

K

K

L

V

F

H

N

S

G

G

Y

R

A

A

N

N

R

I

G

A

T

G

E

E

L

I

G

G

H
|
H

V

T

P

R

L

L

I

P

A

Y

N

D

G

A

E

L

-

K

-

L

-

G

-

M

-

E

-

N

-

A

-

P

-

I

-

F

P

P

C
|
C

A

G

C
|
C

G

K

S

N

I

S

G

G

C
|
C

L

I

E

D

H

N

Y

Y

A

L

S

S

T
x
G

A

R

A

G

L

F

V
x
E

R

Q

R

L

F

Y

S

D

K

H

R

-

I

-

A

-

E

-

A

-

G

-

I

-

S

-

Y

Y

P

F

N

S

E

E

E

K

I

L

N

S

G
x
A

E

P

L
x
E

I

I

V

I

R

A

L
x
H

Y

Y

K

E

Q

Q

G

G

D

E

K

R

I

R

A

A

A

V

E

Q

S

H

L

V

N

E

E

R

H

F

C

M

D

E

F

L

L

L

G

A

H

I

G

C

I

L

A

A

G

N

F

I

I

F

N

T

I

C

F

L

S

D

P

P

Q

H

R

V

V

V

I

L

G

G

G
|
G

L

L

S

S

E
x
N

A

F

G

E

D

L

F

I

Y

Y

I

-

Q

Q

K

E

V

L

S

P

E

-

K

K

A

R

L

L

R

P

Y

A

A

H

I

L

P

L

D

H

C

V

A

A

V

K

N

L

T

P

E

K

I

I

M

I

A

K

A

A

S

R

L

H

G

G

N

D

K

A

A

G

G

G

S

V

I

R

G

G

A

A

S

-

L

-

F

F

L

L

N

N

binding phosphoaminophosphonic acid-adenylate ester: G11 (= G13), T12 (= T14), K13 (≠ S15), G133 (= G142), T134 (= T143), G194 (≠ T192), E198 (≠ V196), A211 (≠ G217), G256 (= G262), G257 (= G263), N260 (≠ E266)
binding zinc ion: H159 (= H168), C180 (= C178), C182 (= C180), C187 (= C185), E213 (≠ L219), H217 (≠ L223)

Q9Y223 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Homo sapiens (Human) (see 18 papers)
32% identity, 87% coverage: 1:277/317 of query aligns to 404:681/722 of Q9Y223

query
sites
Q9Y223

M

L

E

E

K

T

E

L

Y

S

A

A

I

L

G

A

I

V

D
|
D

L

L

G

G

G
|
G

T
|
T

S
x
N

V

L

K
x
R

Y

V

A

A

L

I

I

V

D

S

N

M

N

K

G

G

-

E

-

I

V

V

F

K

H

K

F

Y

Q

T

G

Q

K

F

L

N

P

P

S

K

N

T

A

Y

D

E

V

E

S

R

A

I

E

N

A

L

V

I

I

L

G

Q

Q

M

L

C

V

V

K

E

A

A

V

A

N

A

E

E

V

-

K

-

T

-

F

-

A

A

E

V

A

K

K

L

R

N

Y

C

T

R

I

I

A

L

G

G

I

V

G

G

I
|
I

G

S

T

T

P

G

G
|
G

I
x
R

V

V

D

N

C

P

T

R

N

E

R

G

I

I

V

V

L

L

G

H

G

S

A
x
T

E

K

N

L

I

I

Q

Q

G

E

W

W

E

N

N

S

L

V

K

D

L

L

A

R

D

T

R

P

M

L

E

S

K

D

E

T

T

L

G

H

L

L

P

P

T

V

Q

W

L

V

G

D

N
|
N

D
|
D

A

G

N
|
N

L

C

M

A

G

A

L

L

G
x
A

E

E

T

R

M

K

Y
x
F

G

G

A

Q

G

G

N

K

G

G

A

L

T

E

H

N

V

F

V

V

F

T

L

L

T

I

V

T

G

G

T

T

G
|
G

I

I

G

G

A

G

V

I

I

I

I

H

D

Q

G

H

K

E

L

L

F

I

N

H

G

G

Y

S

A

S

N

F

R

C

G

A

T

A

E
|
E

L

L

G

G

H
|
H

V

L

P

V

L
x
V

I

S

A

L

N

D

G
|
G

E

P

P

D

C
|
C

A

S

C
|
C

G

G

S

S

I

H

G

G

C
|
C

L
x
I

E
|
E

H

A

Y

Y

A

A

S

S

T

G

A

M

A

A

L

L

V

Q

R

R

R

E

F

A

S

K

K

K

R

L

I

H

A

D

E

E

-

D

-

L

-

V

A

E

G

G

I

M

S

S

Y

V

P

P

N

K

E

D

E

E

I

A

N

V

G

G

E

A

L

L

-

H

I

L

V

I

R

Q

L
x
A

Y
x
A

K

K

Q

L

G

G

D

N

K

A

I

K

A

A

A

Q

E

S

S

I

L

L

N

R

E

T

H

A

C

G

D

T

F

A

L

L

G

G

H

L

G

G

I

V

A

V

G

N

F

I

I

L

N

H

I

T

F

M

S

N

P

P

Q

S

R

L

V

V

V

I

I

L

G

S

G

G

G

V

L

L

S

A

E

S

A

-

G

-

D

-

F

H

Y

Y

I

I

Q

H

K

I

V

V

S

K

E

D

D413 (= D10) binding
G416 (= G13) binding
T417 (= T14) binding
N418 (≠ S15) binding
R420 (≠ K17) binding
I472 (= I71) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 50% of the wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to less than 10% of wild-type activity
G476 (= G75) binding ; binding
R477 (≠ I76) binding ; binding
T489 (≠ A88) binding ; binding
N516 (= N115) binding ; binding
D517 (= D116) active site; binding ; binding ; mutation D->A,N: Loss of N-acylmannosamine kinase activity. Decreased affinity for N-acyl-D-mannosamine. No effect on structure.
N519 (= N118) to S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs1554658910; mutation to S: Decreased N-acylmannosamine kinase activity.
A524 (≠ G123) to V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 10% of wild-type activity; decreased N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs764698870
F528 (≠ Y127) to C: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70% of wild-type activity; decreased N-acylmannosamine kinase activity; dbSNP:rs986773986; mutation to C: Decreased N-acylmannosamine kinase activity.
G545 (= G144) binding
E566 (= E165) binding
H569 (= H168) binding ; binding ; binding
V572 (≠ L171) to L: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70-80% of wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to less than 10% of wild-type activity; does not affect homohexamers formation; dbSNP:rs121908632
G576 (= G175) to E: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs121908625
C579 (= C178) binding
C581 (= C180) binding
C586 (= C185) binding
I587 (≠ L186) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs748949603; mutation to T: Decreased N-acylmannosamine kinase activity.
E588 (= E187) binding ; binding
A630 (≠ L223) to T: in NM; decreased N-acylmannosamine kinase activity; does not affect homohexamers formation; dbSNP:rs1382191649
A631 (≠ Y224) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 80% of wild-type activity; decreased N-acylmannosamine kinase activity; retains 75% of wild-type activity; dbSNP:rs121908626; to V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70% of wild-type activity; decreased N-acylmannosamine kinase activity; does not affect homohexamers formation; dbSNP:rs62541771; mutation A->V,T: Decreased N-acylmannosamine kinase activity.

Sites not aligning to the query:

13 C → S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; dbSNP:rs1209266607
19 binding
23 binding
113 binding
132 H → Q: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 10% of wild-type activity; impaired homohexamers formation
176 D → V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs139425890
177 R → C: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs539332585
200 I → F: in NM; uncertain significance; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 90% of wild-type activity; decreased N-acylmannosamine kinase activity; retains 75% of wild-type activity; dbSNP:rs369328625
206 G → S: in NM; moderate phenotype with unusual involvement of quadriceps; dbSNP:rs766266918
220 binding
253 binding
259 binding
263 R → L: in SIALURIA; strong reduction of feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908623
266 R → Q: in SIALURIA; abolishes feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908622; R → W: in sialuria; dbSNP:rs121908621
271 binding
280 binding
281 binding
282 binding
301 binding
302 binding
303 C → V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 80% of wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to 60% of wild-type activity; requires 2 nucleotide substitutions; dbSNP:rs121908633
307 binding
321 binding
331 V → A: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation
378 D → Y: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 10-30% of wild-type activity; decreased N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs199877522
708 G → S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; severely decreased; dbSNP:rs1554657922
712 M→T: Decreased N-acylmannosamine kinase activity.

O35826 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Rattus norvegicus (Rat) (see paper)
31% identity, 87% coverage: 1:277/317 of query aligns to 404:681/722 of O35826

query
sites
O35826

M

L

E

E

K
x
T

E
x
L

Y
x
S

A
|
A

I
x
L

G
x
A

I
x
V

D
|
D

L
|
L

G
|
G

T
|
T

S
x
N

V
x
L

K
x
R

Y
x
V

A
|
A

L
x
I

I
x
V

D
x
S

N
x
M

N
x
K

G
|
G

-
x
E

-
x
I

V
|
V

F
x
K

H
x
K

F
x
Y

Q
x
T

G
x
Q

K
x
F

L
x
N

P
|
P

S
x
K

N
x
T

A
x
Y

D
x
E

V
x
E

S
x
R

A
x
I

E
x
S

A
x
L

V
x
I

I
x
L

G
x
Q

Q
x
M

L
x
C

V
|
V

K
x
E

A
|
A

V
x
A

N
x
A

E
|
E

V

-

K

-

T

-

F

-

A
|
A

E
x
V

A
x
K

K
x
L

R
x
N

Y
x
C

T
x
R

I
|
I

A
x
L

G
|
G

I
x
V

G
|
G

I
|
I

G
x
S

T
|
T

P
x
G

G
|
G

I
x
R

V
|
V

D
x
N

C
x
P

T
x
Q

N
x
E

R
x
G

I
x
V

V
|
V

L
|
L

G
x
H

G
x
S

A
x
T

E
x
K

N
x
L

I
|
I

Q
|
Q

G
x
E

W
|
W

E
x
N

N
x
S

L
x
V

K
x
D

L
|
L

A
x
R

D
x
T

R
x
P

M
x
L

E
x
S

K
x
D

E
x
T

T
x
L

G
x
H

L
|
L

P
|
P

T
x
V

Q
x
W

L
x
V

G
x
D

N
|
N

D
|
D

A
x
G

N
|
N

L
x
C

M
x
A

G
x
A

L
x
M

G
x
A

E
|
E

T
x
R

M
x
K

Y
x
F

G
|
G

A
x
Q

G
|
G

N
x
K

G
|
G

A
x
Q

T
x
E

H
x
N

V
x
F

V
|
V

F
x
T

L
|
L

T
x
I

V
x
T

G
|
G

T
|
T

G
|
G

I
|
I

G
|
G

A
x
G

V
x
I

I
|
I

I
x
H

D
x
Q

G
x
H

K
x
E

L
|
L

F
x
I

N
x
H

G
|
G

Y
x
S

A
x
S

N
x
F

R
x
C

G
x
A

T
x
A

E
|
E

L
|
L

G
|
G

H
|
H

V
x
L

P
x
V

L
x
V

I
x
S

A
x
L

N
x
D

G
|
G

E
x
P

P
x
D

C
|
C

A
x
S

C
|
C

G
|
G

S
|
S

I
x
H

G
|
G

C
|
C

L
x
I

E
|
E

H
x
A

Y
|
Y

A
|
A

S
|
S

T
x
G

A
x
M

A
|
A

L
|
L

V
x
Q

R
|
R

R
x
E

F
x
A

S
x
K

K
|
K

R
x
L

I
x
H

A
x
D

E
|
E

-
x
D

-
x
L

-
x
V

A
x
E

G
|
G

I
x
M

S
|
S

Y
x
V

P
|
P

N
x
K

E
x
D

E
|
E

I
x
A

N
x
V

G
|
G

E
x
A

L
|
L

-
x
H

I
x
L

V
x
I

R
x
Q

L
x
A

Y
x
A

K
|
K

Q
x
L

G
|
G

D
x
N

K
x
V

I
x
K

A
|
A

A
x
Q

E
x
S

S
x
I

L
|
L

N
x
R

E
x
T

H
x
A

C
x
G

D
x
T

F
x
A

L
|
L

G
|
G

H
x
L

G
|
G

I
x
V

A
x
V

G
x
N

F
x
I

I
x
L

N
x
H

I
x
T

F
x
M

S
x
N

P
|
P

Q
x
S

R
x
L

V
|
V

V
x
I

I
x
L

G
x
S

G
|
G

G
x
V

L
|
L

S
x
A

E
x
S

A

-

G

-

D

-

F
x
H

Y
|
Y

I
|
I

Q
x
H

K
x
I

V
|
V

S
x
R

E
x
D

D413 (= D10) mutation D->K,N: No effect on UDP-N-acetylglucosamine 2-epimerase activity. Does not affect feedback inhibition by CMP-Neu5Ac. Loss of N-acylmannosamine kinase activity. Does not interfere with oligomerization.
R420 (≠ K17) mutation to M: No effect on UDP-N-acetylglucosamine 2-epimerase activity. Does not affect feedback inhibition by CMP-Neu5Ac. Loss of N-acylmannosamine kinase activity. Does not interfere with oligomerization.

Sites not aligning to the query:

1 UDP-N-acetylglucosamine 2-epimerase
49 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Does not interfere with enzyme oligomerization.
110 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Partial reduction of the dimerization process.
132 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Partial reduction of the dimerization process.
155 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Strong reduction of the dimerization process.
157 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Strong reduction of the dimerization process.
406:722 N-acetylmannosamine kinase

3vovB Crystal structure of rok hexokinase from thermus thermophilus (see paper)
31% identity, 95% coverage: 7:308/317 of query aligns to 4:288/298 of 3vovB

query
sites
3vovB

I

V

G

G

I

L

D

D

L

L

G

G

T

T

S

K

V

I

K

A

Y

A

A

G

L

V

I

F

D

D

N

G

N

K

G

-

V

-

F

-

H

-

F

-

Q

-

G

-

K

R

L

L

P

L

S

S

N

K

A

V

D

V

V

V

S

P

A

T

E

P

A

K

V

E

I

G

G

G

Q

E

L

R

V

V

K

A

A

E

V

A

N

L

E

A

V

E

K

A

T

A

F

E

A

R

E

A

A

E

K

R

R

E

Y

A

T

G

I

V

A

R

G

G

-

E

-

A

I

I

G

G

I

L

G

G

T

T

P

P

G

G

I

P

V

L

D

D

C

F

T

R

N

R

R

G

I

V

V

I

L

-

G

-

A

R

E

P

N

N

I

I

Q

P

G

G

W

V

E

Q

N

D

L

F

K

P

L

I

A

R

D

R

R

I

M

L

E

E

K

E

E

A

T

T

G

G

L

R

P

P

T

V

Q

F

L

L

G

E

N

N

D

D

A

A

N

N

L

A

M

A

G

A

L

L

G

A

E

E

T

H

M

H

Y

L

G

G

A

A

G

A

N

Q

G

G

A

E

T

E

H

S

V

S

V

L

F

Y

L

L

T

T

V

V

G

S

T

T

G

G

I

I

G

G

A

G

V

V

I

V

I

L

D

G

G

G

K

R

L

V

F

L

N

R

G

G

Y

E

A

R

N

G

R

Q

G

G

T

G

E

E

L

L

G

G

H
|
H

V

L

P

T

L

L

I

L

A

P

N

G

G

G

E

P

P

A

C
|
C

A

G

C
|
C

G

G

S

L

I

E

G

G

C
|
C

L

L

E

E

H

A

Y

L

A

A

S

A

T

G

A

R

A

A

L

L

V

-

R

-

F

-

S

-

K

E

R

R

I

D

A

A

E

T

A

Y

G

A

I

F

S

Q

Y

R

P

P

N

-

E

-

I

V

N

D

G

T

E

R

L

E

I

L

V

F

R

R

L

L

Y

F

K

Q

Q

A

G

G

D

D

K

P

I

K

A

A

A

E

E

R

S

L

L

V

N

L

E

Q

H

A

C

A

D

R

F

Y

L

V

G

G

H

I

G

G

I

L

A

A

G

S

F

L

I

V

N

K

I

A

F

F

S

D

P

P

Q

G

R

V

V

V

I

L

G

G

L

V

S

A

-

L

E

N

A

A

G

P

D

E

F

G

Y

Y

I

W

Q

E

K

A

V

L

S

L

E

E

K

A

A

Y

L

R

R

R

Y

Y

A

L

I

-

P

-

D

Q

C

G

A

W

V

E

N

A

T

P

E

P

I

L

M

R

A

R

A

A

S

R

L

L

G

G

N

A

K

E

A

A

G

G

S

L

I

L

G

G

A

A

binding zinc ion: H157 (= H168), C167 (= C178), C169 (= C180), C174 (= C185)

2yi1A Crystal structure of n-acetylmannosamine kinase in complex with n- acetyl mannosamine 6-phosphate and adp. (see paper)
30% identity, 88% coverage: 6:284/317 of query aligns to 5:279/308 of 2yi1A

query
sites
2yi1A

A

A

I

L

G

A

I

V

D

D

L

L

G
|
G

T
|
T

S
x
N

V

L

K
x
R

Y

V

A

A

L

I

I

V

D

S

N

M

N

K

G

G

-

E

-

I

V

V

F

K

H

K

F

Y

Q

T

G

Q

K

F

L

N

P

P

S

K

N

T

A

Y

D

E

V

E

S

R

A

I

E

N

A

L

V

I

I

L

G

Q

Q

M

L

C

V

V

K

E

A

A

V

A

N

A

E

E

V

-

K

-

T

-

F

-

A

A

E

V

A

K

K

L

R

N

Y

C

T

R

I

I

A

L

G

G

I

V

G

G

I

I

G

S

T

T

P
x
G

G
|
G

I
x
R

V

V

D

N

C

P

T

R

N

E

R

G

I

I

V

V

L

L

G

H

G
x
S

A
x
T

E

K

N
x
L

I

I

Q

Q

G

E

W

W

E

N

N

S

L

V

K

D

L

L

A

R

D

T

R

P

M

L

E

S

K

D

E

T

T

L

G

H

L

L

P

P

T

V

Q

W

L

V

G

D

N
|
N

D
|
D

A

G

N
|
N

L

C

M

A

G

A

L

L

G

A

E

E

T

R

M

K

Y

F

G

G

A

Q

G

G

N

K

G

G

A

L

T

E

H

N

V

F

V

V

F

T

L

L

T

I

V

T

G
|
G

T
|
T

G
|
G

I
|
I

G

G

G
|
G

A

G

V

I

I

I

I

H

D

Q

G

H

K

E

L

L

F

I

N

H

G

G

Y

S

A

S

N

F

R

C

G

A

T
x
A

E
|
E

L

L

G

G

H
|
H

V

L

P

V

L

V

I

S

A

L

N

D

G

G

E

P

P

D

C
|
C

A

S

C
|
C

G

G

S

S

I

H

G

G

C
|
C

L

I

E
|
E

H

A

Y

Y

A

A

S

S

T
x
G

A

M

A

A

L

L

V

Q

R

R

R

E

F

A

S

K

K

K

R

L

I

H

A

D

E

E

A

D

G

L

I

L

S

L

Y

V

P

E

N

G

E

M

E

S

I

A

N

V

G

G

E

A

L
|
L

-

H

I

L

V

I

R

Q

L

A

Y

A

K

K

Q

L

G

G

D

N

K

A

I

K

A

A

A

Q

E

S

S

I

L

L

N

R

E

T

H

A

C

G

D

T

F

A

L

L

G

G

H

L

G

G

I

V

A

V

G

N

F

I

I

L

N

H

I

T

F

M

S

N

P

P

Q

S

R

L

V

V

V

I

I

L

G

S

G

G

G
x
V

L

L

S

A

E

-

A

-

G

-

D

S

F

H

Y

Y

I

I

Q

H

K

I

V

V

S

K

E

D

K

V

A

I

L

R

R

Q

Y

Q

A

A

I

L

binding adenosine-5'-diphosphate: G11 (= G12), T13 (= T14), N14 (≠ S15), R16 (≠ K17), T140 (= T143), G189 (≠ T192), L216 (= L219), V261 (≠ G263)
binding 2-acetamido-2-deoxy-6-O-phosphono-alpha-D-mannopyranose: G12 (= G13), G71 (≠ P74), G72 (= G75), R73 (≠ I76), S84 (≠ G87), T85 (≠ A88), L87 (≠ N90), N112 (= N115), D113 (= D116), G139 (= G142), T140 (= T143), G141 (= G144), I142 (= I145), E162 (= E165), H165 (= H168), E184 (= E187)
binding calcium ion: N112 (= N115), N115 (= N118), G144 (= G147), A161 (≠ T164)
binding zinc ion: H165 (= H168), C175 (= C178), C177 (= C180), C182 (= C185)

2yhyA Structure of n-acetylmannosamine kinase in complex with n- acetylmannosamine and adp (see paper)
30% identity, 88% coverage: 6:284/317 of query aligns to 5:279/308 of 2yhyA

query
sites
2yhyA

A

A

I

L

G

A

I

V

D

D

L

L

G
|
G

T
|
T

S
x
N

V

L

K
x
R

Y

V

A

A

L

I

I

V

D

S

N

M

N

K

G

G

-

E

-

I

V

V

F

K

H

K

F

Y

Q

T

G

Q

K

F

L

N

P

P

S

K

N

T

A

Y

D

E

V

E

S

R

A

I

E

N

A

L

V

I

I

L

G

Q

Q

M

L

C

V

V

K

E

A

A

V

A

N

A

E

E

V

-

K

-

T

-

F

-

A

A

E

V

A

K

K

L

R

N

Y

C

T

R

I

I

A

L

G

G

I

V

G

G

I

I

G

S

T

T

P

G

G

G

I

R

V

V

D

N

C

P

T

R

N

E

R

G

I

I

V

V

L

L

G

H

G

S

A

T

E

K

N

L

I

I

Q

Q

G

E

W

W

E

N

N

S

L

V

K

D

L

L

A

R

D

T

R

P

M

L

E

S

K

D

E

T

T

L

G

H

L

L

P

P

T

V

Q

W

L

V

G

D

N
|
N

D

D

A

G

N
|
N

L

C

M

A

G

A

L

L

G

A

E

E

T

R

M

K

Y

F

G

G

A

Q

G

G

N

K

G

G

A

L

T

E

H

N

V

F

V

V

F

T

L

L

T

I

V

T

G

G

T
|
T

G

G

I

I

G

G

G
|
G

A

G

V

I

I

I

I

H

D

Q

G

H

K

E

L

L

F

I

N

H

G

G

Y

S

A

S

N

F

R

C

G

A

T
x
A

E

E

L

L

G

G

H
|
H

V

L

P

V

L

V

I

S

A

L

N

D

G

G

E

P

P

D

C
|
C

A

S

C
|
C

G

G

S

S

I

H

G

G

C
|
C

L

I

E

E

H

A

Y

Y

A

A

S

S

T
x
G

A

M

A

A

L

L

V

Q

R

R

R

E

F

A

S

K

K

K

R

L

I

H

A

D

E

E

A

D

G

L

I

L

S

L

Y

V

P

E

N

G

E

M

E

S

I

A

N

V

G

G

E

A

L
|
L

-

H

I

L

V

I

R

Q

L

A

Y

A

K

K

Q

L

G

G

D

N

K

A

I

K

A

A

A

Q

E

S

S

I

L

L

N

R

E

T

H

A

C

G

D

T

F

A

L

L

G

G

H

L

G

G

I

V

A

V

G

N

F

I

I

L

N

H

I

T

F

M

S

N

P

P

Q

S

R

L

V

V

V

I

I

L

G

S

G

G

G
x
V

L

L

S

A

E

-

A

-

G

-

D

S

F

H

Y

Y

I

I

Q

H

K

I

V

V

S

K

E

D

K

V

A

I

L

R

R

Q

Y

Q

A

A

I

L

binding adenosine-5'-diphosphate: G11 (= G12), G12 (= G13), T13 (= T14), N14 (≠ S15), R16 (≠ K17), T140 (= T143), G189 (≠ T192), L216 (= L219), V261 (≠ G263)
binding calcium ion: N112 (= N115), N115 (= N118), G144 (= G147), A161 (≠ T164)
binding zinc ion: H165 (= H168), C175 (= C178), C177 (= C180), C182 (= C185)

P32718 D-allose kinase; Allokinase; EC 2.7.1.55 from Escherichia coli (strain K12) (see paper)
27% identity, 99% coverage: 1:314/317 of query aligns to 1:299/309 of P32718

query
sites
P32718

M

M

E

Q

K

K

E

Q

Y

H

A

N

I

V

-

V

-

A

G

G

I

V

D

D

L

M

G

G

G

A

T

T

S

H

V

I

K

R

Y

F

A

C

L

L

I

R

D

T

N

A

N

E

G

G

-

E

V

T

F

L

H

H

F

C

Q

E

G

K

K

K

L

-

P

-

S

R

N

T

A

A

D

E

V

V

S

I

A

A

E

P

A

G

V

L

I

V

G

S

Q

G

L

I

V

G

K

E

A

M

V

I

N

D

E

E

V

-

K

-

T

-

F

-

A

-

E

Q

A

L

K

R

R

R

Y

F

T

N

-

A

-

R

I

C

A

H

G

G

I

L

G

V

I

M

G

G

T

F

P

P

G
x
A

I

L

V

V

D

S

C

K

T

D

N

K

R

R

I

T

V

I

L

I

G

S

G

T

A

P

E

N

-

L

N

P

I

L

Q

T

G

A

W

A

E

D

N

L

L

Y

K

D

L

L

A

A

D

D

R

K

M

L

E

E

K

N

E

T

T

L

G

N

L

C

P

P

T

V

Q

E

L

F

G

S

N

R

D

D

A

V

N

N

L

L

M

Q

G

L

L

S

G

W

E

D

T

V

M

V

Y

E

G

-

A

-

G

-

N

N

G

R

A

L

T

T

H

Q

V

Q

V

L

F

V

L

L

T

A

-

A

-

Y

V

L

G

G

T

T

G

G

I

M

G

G

G
x
F

A

A

V

V

I

W

I

M

D

N

G

G

K

A

L

P

F

W

N

T

G

G

Y

A

A

H

N

G

R

V

G

A

T

G

E

E

L

L

G

G

H

H

V

I

P

P

L

L

I

G

A

D

N

M

G

T

E

Q

P

H

C

C

A

A

C

C

G

G

S

N

I

P

G

G

C

C

L

L

E

E

H

T

Y

N

A

C

S

S

T

G

A

M

A

A

L

L

V

R

R

R

R

W

F

Y

S

E

K

Q

R

Q

I

P

A

R

E

-

A

-

G

-

I

-

S

N

Y

Y

P

P

N

L

E

R

E

D

I

L

N

-

G

-

E

-

L

-

I

-

V

-

R

-

L

F

Y

V

K

H

Q

A

G

E

D

N

K

A

I

P

A

F

A

V

E

Q

S

S

L

L

N

L

E

E

H

N

C

A

D

-

F

-

L

-

G

A

H

R

G

A

I

I

A

A

G

T

F

S

I

I

N

N

I

L

F

F

S

D

P

P

Q

D

R

A

V

V

V

I

I

L

G

G

L

V

S

M

E

D

A

M

G

P

D

A

F

F

-

P

-

R

-

E

Y

T

I

L

Q

V

K

A

V

M

S

T

E

Q

K

K

A

Y

L

L

R

R

Y

R

A

P

I

L

P

P

D

H

C

Q

A

V

V

V

N

-

T

-

E

R

I

F

M

I

A

A

S

S

L

S

G

S

N

D

K

F

A

N

G

G

S

A

I

Q

G

G

A

A

S

I

L

L

F

A

L

H

N

Q

R

R

A73 (≠ G75) mutation to G: 60-fold increase in catalytic efficiency for glucose phosphorylation. 45-fold increase in D-glucose affinity. No change in catalytic efficiency for D-allose phosphorylation.
F145 (≠ G147) mutation to L: 10-fold increase in catalytic efficiency for glucose phosphorylation. Slight increase in catalytic efficiency for D-allose phosphorylation.

2yhwA High-resolution crystal structures of n-acetylmannosamine kinase: insights about substrate specificity, activity and inhibitor modelling. (see paper)
31% identity, 88% coverage: 6:284/317 of query aligns to 5:280/309 of 2yhwA

query
sites
2yhwA

A

A

I

L

G

A

I

V

D

D

L

L

G

G

T

T

S

N

V

L

K

R

Y

V

A

A

L

I

I

V

D

S

N

M

N

K

G

G

-

E

-

I

V

V

F

K

H

K

F

Y

Q

T

G

Q

K

F

L

N

P

P

S

K

N

T

A

Y

D

E

V

E

S

R

A

I

E

N

A

L

V

I

I

L

G

Q

Q

M

L

C

V

V

K

E

A

A

V

A

N

A

E

E

V

-

K

-

T

-

F

-

A

A

E

V

A

K

K

L

R

N

Y

C

T

R

I

I

A

L

G

G

I

V

G

G

I

I

G

S

T

T

P

G

G

G

I

R

V

V

D

N

C

P

T

R

N

E

R

G

I

I

V

V

L

L

G

H

G

S

A

T

E

K

N

L

I

I

Q

Q

G

E

W

W

E

N

N

S

L

V

K

D

L

L

A

R

D

T

R

P

M

L

E

S

K

D

E

T

T

L

G

H

L

L

P

P

T

V

Q

W

L

V

G

D

N
|
N

D

D

A

G

N
|
N

L

C

M

A

G

A

L

L

G

A

E

E

T

R

M

K

Y

F

G

G

A

Q

G

G

N

K

G

G

A

L

T

E

H

N

V

F

V

V

F

T

L

L

T

I

V

T

G

G

T

T

G

G

I

I

G

G

G
|
G

A

G

V

I

I

I

I

H

D

Q

G

H

K

E

L

L

F

I

N

H

G

G

Y

S

A

S

N

F

R

C

G

A

T
x
A

E

E

L

L

G

G

H
|
H

V

L

P

V

L

V

I

S

A

L

N

N

G

G

E

P

P

D

C
|
C

A

S

C
|
C

G

G

S

S

I

H

G

G

C
|
C

L

I

E

E

H

A

Y

Y

A

A

S

S

T

G

A

M

A

A

L

L

V

Q

R

R

R

E

F

A

S

K

K

K

R

L

I

H

A

D

E

E

A

D

G

L

I

L

S

L

Y

V

P

E

N

G

E

M

E

S

I

V

N

A

-

V

G

G

E

A

L

L

-

H

I

L

V

I

R

Q

L

A

Y

A

K

K

Q

L

G

G

D

N

K

A

I

K

A

A

A

Q

E

S

S

I

L

L

N

R

E

T

H

A

C

G

D

T

F

A

L

L

G

G

H

L

G

G

I

V

A

V

G

N

F

I

I

L

N

H

I

T

F

M

S

N

P

P

Q

S

R

L

V

V

V

I

I

L

G

S

G

G

G

V

L

L

S

A

E

-

A

-

G

-

D

S

F

H

Y

Y

I

I

Q

H

K

I

V

V

S

K

E

D

K

V

A

I

L

R

R

Q

Y

Q

A

A

I

L

binding calcium ion: N112 (= N115), N115 (= N118), G144 (= G147), A161 (≠ T164)
binding zinc ion: H165 (= H168), C175 (= C178), C177 (= C180), C182 (= C185)

4db3A 1.95 angstrom resolution crystal structure of n-acetyl-d-glucosamine kinase from vibrio vulnificus.
30% identity, 97% coverage: 8:315/317 of query aligns to 12:310/311 of 4db3A

query
sites
4db3A

G

G

I

F

D

D

L

V

G

G

T

T

S

K

V

I

K

E

Y

F

A

G

L

A

I

F

D

N

N

E

N

K

G

L

V

E

F

R

H

V

F

A

Q

T

G

E

K

R

L

V

P

P

S

T

N

P

A

T

D

D

-

D

-

Y

-

P

V

L

S

L

A

L

E

E

A

T

V

I

I

A

G

G

Q

L

L

V

V

A

K

K

A

Y

V

D

N

Q

E

E

V

F

K

A

T

C

F

-

A

-

E

E

A

G

K

K

R

-

Y

-

T

-

I

-

A

-

G

-

I

I

G

G

I

L

G

G

T

L

P

P

G

G

I

M

V

E

D

D

C

A

T

D

N

D

R

A

I

T

V

V

L

L

G

-

A

T

E

V

N

N

I

V

Q

P

G

A

W

A

E

K

N

G

L

K

K

P

L

L

A

R

D

A

R

D

M

L

E

E

K

A

E

K

T

I

G

G

L

R

P

S

T

V

Q

K

L

I

G

E

N

N

D

D

A

A

N

N

L

C

M

F

G

A

L

L

G

S

E

E

T

A

M

W

Y

D

G

E

A

E

G

L

N

Q

G

D

A

A

T

P

H

S

V

V

V

M

F

G

L

L

T

I

V

L

G

G

T

T

G

G

I

F

G

G

A

G

V

L

I

I

I

Y

D

E

G

G

K

K

L

V

F

F

N

S

G

G

Y

R

A

N

N

N

R

V

G

A

T

G

E

E

L

L

G

G

H
|
H

V

M

P

R

L

L

-

P

-

L

-

D

-

A

-

W

-

F

-

H

I

L

A

G

N

D

G

N

E

A

P

P

-

L

-

G

C
|
C

A

G

C
|
C

G

G

S

K

I

K

G

G

C
|
C

L

L

E

D

H

S

Y

Y

A

L

S

S

T

G

A

-

L

-

V

-

R

R

R

G

F

F

S

E

K

L

R

L

I

Y

A

A

E

H

A

-

G

-

I

-

S

-

Y

Y

P

Y

N

G

E

E

I

K

N

K

G

A

E

I

L

D

I

I

V

I

R

K

L

A

Y

N

K

A

Q

A

G

G

D

D

K

E

I

K

A

A

E

E

S

H

L

V

N

E

E

R

H

F

C

M

D

E

F

L

L

L

G

A

H

-

G

-

I

-

A

I

G

C

F

F

I

G

N

N

I

I

F

F

S

T

-

A

-

N

-

D

P

P

Q

H

R

V

V

V

V

A

I

L

G

G

L

L

S

S

E

N

A

F

G

E

D

L

F

I

Y

Y

I

-

Q

E

K

E

V

M

S

P

E

K

K

R

A

V

L

P

R

K

Y

Y

A

L

I

L

P

-

D

S

C

V

A

A

V

K

N

C

T

P

E

K

I

I

M

I

A

K

A

A

S

K

L

H

G

G

N

D

K

S

A

G

G

G

S

V

I

R

G

G

A

A

S

-

L

-

F

F

L

L

N

N

R

I

K

K

binding zinc ion: H165 (= H168), C185 (= C178), C187 (= C180), C192 (= C185)

Query Sequence

>354180 FitnessBrowser__Btheta:354180
MEKEYAIGIDLGGTSVKYALIDNNGVFHFQGKLPSNADVSAEAVIGQLVKAVNEVKTFAE
AKRYTIAGIGIGTPGIVDCTNRIVLGGAENIQGWENLKLADRMEKETGLPTQLGNDANLM
GLGETMYGAGNGATHVVFLTVGTGIGGAVIIDGKLFNGYANRGTELGHVPLIANGEPCAC
GSIGCLEHYASTAALVRRFSKRIAEAGISYPNEEINGELIVRLYKQGDKIAAESLNEHCD
FLGHGIAGFINIFSPQRVVIGGGLSEAGDFYIQKVSEKALRYAIPDCAVNTEIMAASLGN
KAGSIGAASLFLNRKPN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory