SitesBLAST

C108 (≠ I112) mutation to S: No change in activity.
C143 (≠ S142) mutation to S: 30% of wild-type sugar phosphate transport activity.
C265 (≠ A256) mutation to S: No change in activity.
C331 (≠ L323) mutation to S: No change in activity.

Sites not aligning to the query:

436 C→S: No change in activity.
438 C→S: No change in activity.

P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
21% identity, 62% coverage: 30:311/452 of query aligns to 12:301/430 of P0AA76

query
sites
P0AA76

R

R

I

R

R

R

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Y

A

L

T

T

F

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L

V

S

M

A

I

T

F

T

I

G

T

Y

V

G

V

I

I

Y

C

Y
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Y

V

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C
x
D

R
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R

L

A

S

N

M

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N

A

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V

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K

A

P

H

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I

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Q

E

E

D

E

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I

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Y
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Y

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N

G

G

G

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L

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D

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N

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Y

S

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T

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A

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A

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T

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A

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G

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F

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x
E

S

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A

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N

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A

Y

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Q

N

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P

R

Q

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S

T

Q

K

G

G

-

I

-

S

-

K

-

A

-

E

-

L

-

D

F

Y

L

I

L

R

D

D

R

G

G

G

T

L

S

V

D

D

A

G

H

D

S

A

V

P

S

V

G

K

K

K

Q

E

T

A

E

R

E

Q

F

P

N

L

K

T

A

A

Q

K

K

D

A

-

V

-

L

-

K

-

N

-

P

-

A

-

I

-

W

W

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L

A

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-

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-

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N

A

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S

-

T

-

L

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-

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-

L

-

T

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N

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Y

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A

D

D

Y29 (= Y47) binding
D31 (≠ C49) mutation to N: Loss of galactonate transport activity.
R32 (= R50) binding
Y64 (= Y82) binding
E118 (≠ Q136) mutation to Q: Loss of galactonate transport activity.

Sites not aligning to the query:

358 binding

6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
21% identity, 62% coverage: 30:311/452 of query aligns to 1:282/409 of 6e9nA

query
sites
6e9nA

R

R

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x
Q

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N

G

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x
F

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N

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G

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-

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N

A

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-

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M

-

Y

Y

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N

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-

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-

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-

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G

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N

Y

Y

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Q

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K

K

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A

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K

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A

D

D

binding D-gluconic acid: Q57 (≠ K86), F111 (≠ A140)

Sites not aligning to the query:

binding D-gluconic acid: 359

6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
20% identity, 62% coverage: 30:311/452 of query aligns to 4:266/393 of 6e9oA

query
sites
6e9oA

R

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R

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Y

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R

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x
F

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x
Q

N

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D

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D

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H

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S

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S

K

K

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A

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I

K

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K

G

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W

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A

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Y

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x
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x
A

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A

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K

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T

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K

A

A

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D

D

binding D-galactonic acid: Y21 (= Y47), R24 (= R50), Y56 (= Y82), F114 (≠ A140), Q141 (≠ H167), Q228 (≠ R266), A232 (≠ N270)

Sites not aligning to the query:

binding D-galactonic acid: 320, 343

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
19% identity, 55% coverage: 68:315/452 of query aligns to 106:354/495 of Q9NRA2

query
sites
Q9NRA2

E

E

T

T

Q

Q

L

G

G

W

I

I

I

L

G

G

S

S

C

-

L

F

F

F

F

Y

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G

Y

Y

A

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G

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K

Q

L

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P

N

G

G

G

F

Y

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A

A

D

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K

S

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N

G

V

G

K
|
K

R

M

F

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M

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F

G

G

L

I

L

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C

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A

A

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A

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D

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-

G

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A

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E

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x
H

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E

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R

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K

F
x
L

Y
x
L

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A

A

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A

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S

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x
R

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x
N

K

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K

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K

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W

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A

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A

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Y

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Y

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Y

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K

A

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K

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Y

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T

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D

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A

N

S
x
G

F

F

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S

S

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S
x
P

S

Y

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C

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M

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D

D

K

N

F

L

F

R

A

A

K136 (= K99) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ V144) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ FY 159:160) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ DA 227:228) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ HSVSG 229:233) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (≠ S289) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (≠ S295) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794
371 G → V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Q9DC37 Major facilitator superfamily domain-containing protein 1 from Mus musculus (Mouse) (see 2 papers)
23% identity, 71% coverage: 90:408/452 of query aligns to 99:414/464 of Q9DC37

query
sites
Q9DC37

G

G

F

F

L

L

A

I

D

D

R

R

S

I

N

F

V

G

K

I

R

R

F

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M

G

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-

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-

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Sites not aligning to the query:

11:12 Dileucine internalization motif; LL→AA: Mislocalization to the plasma membrane. Localizes to lysosomes when expressed with wild-type Glmp.
76 N→A: No change in molecular weight, indicating lack of N-glycosylation.
449 N→A: No change in molecular weight, indicating lack of N-glycosylation.

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
24% identity, 60% coverage: 90:360/452 of query aligns to 78:357/452 of Q5EXK5

query
sites
Q5EXK5

G

G

F

P

L

L

A

S

D
|
D

R

R

S

F

N

G

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K

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T

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x
V

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D82 (= D94) mutation to A: Loss of activity.
V311 (≠ I308) mutation to W: Loss of activity.
D314 (= D311) mutation to A: Loss of activity.

Query Sequence

>354254 FitnessBrowser__Btheta:354254
MGFKLLDFYKISPPVSGGETDSERSVRFKRIRWATFLSATTGYGIYYVCRLSMNVIRKPI
VEDGVFTETQLGIIGSCLFFVYAVGKLTNGFLADRSNVKRFMSTGLLCSALINLCLGFTN
SFFAFVLLWGLNGWFQSMGAASGVVSLTRWYSSKERGTFYGFWSASHNLGEALTFISIAL
LVSWMGWRYGMIGAGVIGLLGFLMMLAFMRDTPQSQGFLLDRRGTSDAHSVSGKQTEEFN
KAQKAVLKNPAIWILALSSAFMYISRYAVNSWGVFYLEAQKGYSTLDASFIISISSVCGI
VGTVFSGIISDKFFAGSRNVPALIFGLMNVSALCLFLLVPGVHFWVDALAMVLFGLGIGV
LICFLGGLMAVDIAPCNASGAALGVVGIASYIGAGLQDVMSGILIEGQKTVQNGVEVYDF
TYINWFWIGAALLSVVFALLVWNAGEKRSEIN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory