SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3607131 FitnessBrowser__Dino:3607131 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3uf0A Crystal structure of a putative NAD(p) dependent gluconate 5- dehydrogenase from beutenbergia cavernae(efi target efi-502044) with bound NADP (low occupancy)
39% identity, 96% coverage: 7:252/255 of query aligns to 3:246/249 of 3uf0A

query
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3uf0A

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active site: G18 (= G22), S141 (= S147), V151 (≠ H157), Y154 (= Y160), K158 (= K164)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G18), S17 (= S21), G18 (= G22), I19 (= I23), R39 (= R43), D63 (= D68), L64 (= L69), N89 (≠ S95), G91 (= G97), I92 (= I98), I139 (= I145), A140 (≠ S146), S141 (= S147), Y154 (= Y160), K158 (= K164), P184 (= P190), G185 (≠ T191), V187 (= V193), T189 (= T195), N191 (≠ M197), T192 (≠ G198)

3o03A Quaternary complex structure of gluconate 5-dehydrogenase from streptococcus suis type 2 (see paper)
40% identity, 97% coverage: 5:251/255 of query aligns to 5:245/254 of 3o03A

query
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3o03A

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active site: G22 (= G22), S147 (= S147), V157 (≠ H157), Y160 (= Y160), K164 (= K164)
binding calcium ion: S147 (= S147), M148 (≠ Q148), P190 (= P190)
binding D-gluconic acid: I99 (≠ A99), R101 (≠ T101), S147 (= S147), M149 (≠ T149), R154 (≠ L154), Y160 (= Y160)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G18), Y21 (≠ S21), G22 (= G22), I23 (= I23), D42 (= D44), I43 (≠ L45), L47 (= L48), D68 (= D68), V69 (≠ L69), N95 (≠ S95), A96 (= A96), G97 (= G97), I145 (= I145), Y160 (= Y160), K164 (= K164), P190 (= P190)

5u9pB Crystal structure of a gluconate 5-dehydrogenase from burkholderia cenocepacia j2315 in complex with NADP and tartrate
37% identity, 98% coverage: 5:254/255 of query aligns to 10:259/261 of 5u9pB

query
sites
5u9pB

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active site: G27 (= G22), S152 (= S147), Y165 (= Y160), K169 (= K164)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G23 (= G18), R26 (≠ S21), G27 (= G22), I28 (= I23), R48 (= R43), D73 (= D68), V74 (≠ L69), N100 (≠ S95), A101 (= A96), I150 (= I145), Y165 (= Y160), K169 (= K164), P195 (= P190), F198 (≠ V193), T200 (= T195), L202 (≠ M197), N203 (≠ G198)

5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
34% identity, 97% coverage: 7:254/255 of query aligns to 3:254/255 of 5itvA

query
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5itvA

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active site: G18 (= G22), S141 (= S147), Y154 (= Y160), K158 (= K164)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G18), S17 (= S21), G18 (= G22), I19 (= I23), D38 (= D44), I39 (≠ L45), T61 (≠ G67), I63 (≠ L69), N89 (≠ S95), G91 (= G97), T139 (≠ I145), S141 (= S147), Y154 (= Y160), K158 (= K164), P184 (= P190), G185 (≠ T191), I186 (≠ V192), I187 (≠ V193)

3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
36% identity, 98% coverage: 7:255/255 of query aligns to 4:247/247 of 3op4A

query
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3op4A

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S

K

K

A

A

V

V

M

L

P

R

G

G

M

M

Q

K

R

K

K

R

Q

Q

G

G

K

R

I

I

N

N

I
x
V

S

G

S
|
S

Q

V

T

V

G

G

V

T

I

M

A

G

L

N

K

A

D

G

H

Q

A

A

A

N

Y
|
Y

A

A

T

A

S

A

K
|
K

G

A

G

G

L

V

N

I

A

G

L

F

T

T

K

K

S

S

L

M

M

A

T

R

E

E

A

V

A

A

P

S

H

R

N

G

V

V

Q

T

V

V

N

N

A

T

I

V

C

A

P
|
P

T
x
G

V

F

V
x
I

L

E

T
|
T

E

D

M
|
M

G

T

K

K

E

A

L

L

W

-

S

-

A

N

P

D

E

E

R

Q

K

R

D

T

P

A

F

T

I

L

A

A

R

Q

T

V

P

P

L

A

G

G

R

R

F

L

G

G

E

D

P

P

I

R

E

E

I

I

A

A

D

S

M

A

A

V

L

A

Y

F

L

L

A

A

S

S

P

P

A

E

S

A

D

A

L

Y

V

I

N

T

G

G

A

E

V

T

M

L

M

H

I

V

E

N

G

G

Y

M

S

Y

S

M

I

I

binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G18), S17 (= S20), R18 (≠ S21), I20 (= I23), T40 (≠ R43), N62 (≠ D68), V63 (≠ L69), N89 (≠ S95), A90 (= A96), I92 (= I98), V139 (≠ I145), S141 (= S147), Y154 (= Y160), K158 (= K164), P184 (= P190), G185 (≠ T191), I187 (≠ V193), T189 (= T195), M191 (= M197)

4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
36% identity, 98% coverage: 7:255/255 of query aligns to 4:243/243 of 4i08A

query
sites
4i08A

F

M

S

N

L

L

Q

E

D

G

K

K

T

V

A

A

L

L

V

V

T

T

G
|
G

A

A

S
|
S

S
x
R

G
|
G

I
|
I

G

G

R

K

A

A

I

I

A

A

E

E

V

L

F

L

A

A

D

E

A

R

G

G

A

A

D

K

I

V

V

I

G

G

Q

T

G

A

R
x
T

D

S

L

E

D

S

R

G

L

A

T

Q

D

A

L

I

G

S

A

D

Q

Y

I

L

K

G

T

D

T

N

G

G

R

K

Q

G

F

M

A

A

A

L

I

-

T

-

G

-

D
x
N

L
x
V

A

T

D

N

P

P

D

E

Q

S

T

I

Q

E

N

A

V

V

A

L

D

K

R

A

A

I

L

T

A

D

A

E

F

F

G

G

K

G

I

V

D

D

I

I

L

L

V

V

N

N

S
x
N

A
|
A

G

G

I

I

A

T

V

R

T

D

G

N

P

L

V

L

T

M

N

R

Y

M

D

K

L

E

D

E

W

W

Q

S

R

D

T

I

L

M

A

E

V

T

N
|
N

L

L

T

T

A

S

P

I

F

F

I

R

L

L

S

S

K

K

A

A

V

V

M

L

P

R

G

G

M

M

Q

K

R

K

K

R

Q

Q

G

G

K

R

I

I

N

N

I

V

S
x
G

S
|
S

Q

V

T

V

G

G

V

T

I

M

A

G

L

N

K

A

D

G

H
x
Q

A

A

A

N

Y
|
Y

A

A

T

A

S

A

K
|
K

G

A

G

G

L

V

N

I

A

G

L

F

T

T

K

K

S

S

L

M

M

A

T

R

E

E

A

V

A

A

P

S

H

R

N

G

V

V

Q

T

V

V

N

N

A

T

I

V

C

A

P
|
P

T
x
G

V

F

V

I

L

E

T
|
T

E

D

M

M

G

N

K

D

E

E

L

Q

W

R

S

T

A

A

P

T

E

-

R

-

K

-

D

-

P

-

F

-

I

L

A

A

R

Q

T

V

P

P

L

A

G

G

R

R

F

L

G

G

E

D

P

P

I

R

E

E

I

I

A

A

D

S

M

A

A

V

L

A

Y

F

L

L

A

A

S

S

P

P

A

E

S

A

D

A

L

Y

V

I

N

T

G

G

A

E

V

T

M

L

M

H

I

V

E

N

G

G

Y

M

S

Y

S

M

I

I

active site: G19 (= G22), N113 (= N119), S141 (= S147), Q151 (≠ H157), Y154 (= Y160), K158 (= K164)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G18), S17 (= S20), R18 (≠ S21), I20 (= I23), T40 (≠ R43), N62 (≠ D68), V63 (≠ L69), N89 (≠ S95), A90 (= A96), G140 (≠ S146), S141 (= S147), Y154 (= Y160), K158 (= K164), P184 (= P190), G185 (≠ T191), T189 (= T195)

3d3wA Structure of l-xylulose reductase with bound coenzyme, phosphate and hydroxide. (see paper)
35% identity, 96% coverage: 9:252/255 of query aligns to 5:241/244 of 3d3wA

query
sites
3d3wA

L

L

Q

A

D

G

K

R

T

R

A

V

L

L

V

V

T

T

G
|
G

A

A

S
x
G

S
x
K

G
|
G

I
|
I

G

G

R

R

A

G

I

T

A

V

E

Q

V

A

F

L

A

H

D

A

A

T

G

G

A

A

D

R

I

V

V

V

G

A

Q

V

G
x
S

R
|
R

-
x
T

-

Q

-

A

D

D

L

L

D

D

R

S

L

L

T

V

D

R

L

E

G

C

A

P

Q

G

I

I

K

E

T

-

G

-

R

-

Q

-

F

-

A

-

A

P

I

V

T

C

G
x
V

D

D

L
|
L

A

G

D

D

P

W

D

E

Q

A

T

T

Q

-

N

-

V

-

A

-

D

E

R

R

A

A

L

L

A

G

A

S

F

V

G

G

K

P

I

V

D

D

I

L

L

L

V

V

N

N

S
x
N

A
|
A

G
x
A

I

V

A

A

V

L

T

L

G

Q

P

P

V

F

T

L

N

E

Y

V

D

T

L

K

D

E

D

A

W

F

Q

D

R

R

T

S

L

F

A

E

V
|
V

N

N

L

L

T

R

A

A

P

V

F

I

I

Q

L

V

S

S

K

Q

A

I

V

V

M

A

P

R

G

G

M

L

M

I

Q

A

R

R

K

G

-

V

Q

P

G

G

K

A

I

I

I

V

N

N

I
x
V

S
|
S

Q

Q

T

C

G

S

V

Q

I

R

A

A

L

V

K

T

D

N

H
|
H

A

S

A

V

Y
|
Y

A

C

T

S

S

T

K
|
K

G

G

G

A

L

L

N

D

A

M

L

L

T

T

K

K

S

V

L

M

M

A

T

L

E

E

A

L

A

G

P

P

H

H

N

K

V

I

Q

R

V

V

N

N

A

A

I

V

C

N

P
|
P

T
|
T

V

V

V
|
V

L

M

T
|
T

E
x
S

M
|
M

G
|
G

K

Q

E

A

L

T

W

W

S

S

A

D

P

P

E

H

R

K

K

A

D

K

P

T

F

M

I

L

A

N

R

R

T

I

P

P

L

L

G

G

R

K

F

F

G

A

E

E

P

V

I

E

E

H

I

V

A

V

D

N

M

A

A

I

L

L

Y

F

L

L

A

L

S

S

P

D

A

R

S

S

D

G

L

M

V

T

N

T

G

G

A

S

V

T

M

L

M

P

I

V

E

E

G

G

Y

F

active site: G18 (= G22), S136 (= S147), H146 (= H157), Y149 (= Y160), K153 (= K164)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G18), G16 (≠ S20), K17 (≠ S21), G18 (= G22), I19 (= I23), S38 (≠ G42), R39 (= R43), T40 (vs. gap), V59 (≠ G67), L61 (= L69), N83 (≠ S95), A84 (= A96), A85 (≠ G97), V106 (= V118), V134 (≠ I145), S135 (= S146), S136 (= S147), Y149 (= Y160), K153 (= K164), P179 (= P190), T180 (= T191), V182 (= V193), T184 (= T195), S185 (≠ E196), M186 (= M197), G187 (= G198)

1pr9A Human l-xylulose reductase holoenzyme (see paper)
35% identity, 96% coverage: 9:252/255 of query aligns to 5:241/244 of 1pr9A

query
sites
1pr9A

L

L

Q

A

D

G

K

R

T

R

A

V

L

L

V

V

T

T

G
|
G

A

A

S
x
G

S
x
K

G
|
G

I
|
I

G

G

R

R

A

G

I

T

A

V

E

Q

V

A

F

L

A

H

D

A

A

T

G

G

A

A

D

R

I

V

V

V

G

A

Q

V

G
x
S

R
|
R

-
x
T

-

Q

-

A

D

D

L

L

D

D

R

S

L

L

T

V

D

R

L

E

G

C

A

P

Q

G

I

I

K

E

T

-

G

-

R

-

Q

-

F

-

A

-

A

P

I

V

T

C

G

V

D

D

L
|
L

A

G

D

D

P

W

D

E

Q

A

T

T

Q

-

N

-

V

-

A

-

D

E

R

R

A

A

L

L

A

G

A

S

F

V

G

G

K

P

I

V

D

D

I

L

L

L

V

V

N

N

S
x
N

A

A

G
x
A

I

V

A

A

V

L

T

L

G

Q

P

P

V

F

T

L

N

E

Y

V

D

T

L

K

D

E

D

A

W

F

Q

D

R

R

T

S

L

F

A

E

V
|
V

N

N

L

L

T

R

A

A

P

V

F

I

I

Q

L

V

S

S

K

Q

A

I

V

V

M

A

P

R

G

G

M

L

M

I

Q

A

R

R

K

G

-

V

Q

P

G

G

K

A

I

I

I

V

N

N

I
x
V

S

S

S
|
S

Q

Q

T
x
C

G

S

V

Q

I

R

A

A

L

V

K

T

D

N

H
|
H

A

S

A

V

Y
|
Y

A

C

T

S

S

T

K
|
K

G

G

G

A

L

L

N

D

A

M

L

L

T

T

K

K

S

V

L

M

M

A

T

L

E

E

A

L

A

G

P

P

H

H

N

K

V

I

Q

R

V

V

N

N

A

A

I

V

C

N

P

P

T
|
T

V

V

V
|
V

L

M

T
|
T

E

S

M
|
M

G

G

K

Q

E

A

L

T

W

W

S

S

A

D

P

P

E

H

R

K

K

A

D

K

P

T

F

M

I

L

A

N

R

R

T

I

P

P

L

L

G

G

R

K

F

F

G

A

E

E

P

V

I

E

E

H

I

V

A

V

D

N

M

A

A

I

L

L

Y

F

L

L

A

L

S

S

P

D

A

R

S

S

D

G

L

M

V

T

N

T

G

G

A

S

V

T

M

L

M

P

I

V

E

E

G

G

Y

F

active site: G18 (= G22), S136 (= S147), H146 (= H157), Y149 (= Y160), K153 (= K164)
binding dihydrogenphosphate ion: S136 (= S147), C138 (≠ T149)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G18), G16 (≠ S20), K17 (≠ S21), G18 (= G22), I19 (= I23), S38 (≠ G42), R39 (= R43), T40 (vs. gap), L61 (= L69), N83 (≠ S95), A85 (≠ G97), V106 (= V118), V134 (≠ I145), Y149 (= Y160), K153 (= K164), T180 (= T191), V182 (= V193), T184 (= T195), M186 (= M197)

Q7Z4W1 L-xylulose reductase; XR; Carbonyl reductase II; Dicarbonyl/L-xylulose reductase; Kidney dicarbonyl reductase; kiDCR; Short chain dehydrogenase/reductase family 20C member 1; Sperm surface protein P34H; EC 1.1.1.10 from Homo sapiens (Human) (see 2 papers)
35% identity, 96% coverage: 9:252/255 of query aligns to 5:241/244 of Q7Z4W1

query
sites
Q7Z4W1

L

L

Q

A

D

G

K

R

T

R

A

V

L
|
L

V
|
V

T
|
T

G
|
G

A
|
A

S
x
G

S
x
K

G
|
G

I
|
I

G
|
G

R
|
R

A
x
G

I
x
T

A
x
V

E
x
Q

V
x
A

F
x
L

A
x
H

D
x
A

A
x
T

G
|
G

A
|
A

D
x
R

I
x
V

V
|
V

G
x
A

Q
x
V

G
x
S

R
|
R

-
x
T

-

Q

-

A

D

D

L

L

D

D

R

S

L

L

T

V

D

R

L

E

G

C

A

P

Q

G

I

I

K

E

T

-

G

-

R

-

Q

-

F

-

A

-

A

P

I

V

T

C

G

V

D

D

L

L

A

G

D

D

P

W

D

E

Q

A

T

T

Q

-

N

-

V

-

A

-

D

E

R

R

A

A

L

L

A

G

A

S

F

V

G

G

K

P

I

V

D

D

I

L

L

L

V

V

N

N

S

N

A

A

G

A

I

V

A

A

V

L

T

L

G

Q

P

P

V

F

T

L

N

E

Y

V

D

T

L

K

D

E

D

A

W

F

Q

D

R

R

T

S

L

F

A

E

V

V

N
|
N

L

L

T

R

A

A

P

V

F

I

I

Q

L

V

S

S

K

Q

A

I

V

V

M

A

P

R

G

G

M

L

M

I

Q

A

R

R

K

G

-

V

Q

P

G

G

K

A

I

I

I

V

N

N

I

V

S

S

S
|
S

Q

Q

T

C

G

S

V

Q

I

R

A

A

L

V

K

T

D

N

H

H

A

S

A

V

Y

Y

A

C

T

S

S

T

K
|
K

G

G

G

A

L

L

N

D

A

M

L

L

T

T

K

K

S

V

L

M

M

A

T

L

E

E

A

L

A

G

P

P

H

H

N

K

V

I

Q

R

V

V

N

N

A

A

I

V

C

N

P

P

T

T

V

V

L

M

T

T

E

S

M

M

G

G

K

Q

E

A

L

T

W

W

S

S

A

D

P

P

E

H

R

K

K

A

D

K

P

T

F

M

I

L

A

N

R

R

T

I

P

P

L

L

G

G

R

K

F

F

G

A

E

E

P

V

I

E

E

H

I

V

A

V

D

N

M

A

A

I

L

L

Y

F

L

L

A

L

S

S

P

D

A

R

S

S

D

G

L

M

V

T

N

T

G

G

A

S

V

T

M

L

M

P

I

V

E

E

G

G

Y

F

11:40 (vs. 15:43, 43% identical) binding
N107 (= N119) mutation N->L,D: Loss of function. Probably due to defects in formation of the active site and binding of coenzyme.
S136 (= S147) binding
K153 (= K164) binding

Sites not aligning to the query:

1 modified: N-acetylmethionine

P50163 Tropinone reductase 2; Tropinone reductase II; TR-II; EC 1.1.1.236 from Datura stramonium (Jimsonweed) (Common thornapple) (see paper)
34% identity, 96% coverage: 6:251/255 of query aligns to 4:253/260 of P50163

query
sites
P50163

R

R

F

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N

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L

Q

E

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G

K

C

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T

A

A

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L

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V

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T

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x
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G
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I
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Y

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x
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I
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I

A
x
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E
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E

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E

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F

F

-

H

G

G

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I

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N

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A

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C

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A

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A

A

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D

N

N

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N

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C

G

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L

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P

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-

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G

18:41 (vs. 20:43, 42% identical) binding
S146 (= S147) binding
IATSL 192:196 (≠ VLTEM 193:197) binding

2ae2A Tropinone reductase-ii complexed with NADP+ and pseudotropine (see paper)
34% identity, 96% coverage: 6:251/255 of query aligns to 3:252/259 of 2ae2A

query
sites
2ae2A

R

R

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I

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x
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F

-

H

G

G

K

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I

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N

N

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x
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G

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E

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I

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E

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C

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D

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N

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x
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x
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x
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M
x
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x
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K

E

E

M

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I

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Q

A

D

P

P

E

E

R

Q

K

K

D

E

-

N

-

L

-

N

P

K

F

L

I

I

A

D

R

R

T

C

P

A

L

L

G

R

R

R

F

M

G

G

E

E

P

P

I

K

E

E

I

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A

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A

M

M

A

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A

Y

F

L

L

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P

P

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G

active site: G19 (= G22), S145 (= S147), Y158 (= Y160), K162 (= K164)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G18), S17 (= S20), R18 (≠ S21), G19 (= G22), I20 (= I23), S39 (≠ G42), R40 (= R43), C64 (≠ G67), L66 (= L69), N93 (≠ S95), G95 (= G97), I116 (≠ V118), I143 (= I145), S145 (= S147), Y158 (= Y160), K162 (= K164), P188 (= P190), G189 (≠ T191), V190 (= V192), I191 (≠ V193), T193 (= T195), S194 (≠ E196), L195 (≠ M197), V196 (≠ G198)
binding pseudotropine: S145 (= S147), E155 (≠ H157), Y158 (= Y160), L195 (≠ M197)

1ipfA Tropinone reductase-ii complexed with NADPH and tropinone (see paper)
34% identity, 96% coverage: 6:251/255 of query aligns to 3:252/259 of 1ipfA

query
sites
1ipfA

R

R

F

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N

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L

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x
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G

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H

G

G

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x
N

A

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x
S

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G

active site: G19 (= G22), S145 (= S147), Y158 (= Y160), K162 (= K164)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G18), R18 (≠ S21), G19 (= G22), I20 (= I23), S39 (≠ G42), R40 (= R43), C64 (≠ G67), D65 (= D68), L66 (= L69), N93 (≠ S95), S145 (= S147), Y158 (= Y160), K162 (= K164), P188 (= P190), V190 (= V192), I191 (≠ V193), T193 (= T195), S194 (≠ E196), L195 (≠ M197), V196 (≠ G198)
binding 8-methyl-8-azabicyclo[3,2,1]octan-3-one: S147 (≠ T149), E155 (≠ H157), Y158 (= Y160)

1ipeA Tropinone reductase-ii complexed with NADPH (see paper)
34% identity, 96% coverage: 6:251/255 of query aligns to 3:252/259 of 1ipeA

query
sites
1ipeA

R

R

F

W

S

N

L

L

Q

E

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C

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T

A

A

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G

A

G

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S

S
x
R

G
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G

I
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I

G

G

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Y

A

G

I

I

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E

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A

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x
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L

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L

A

A

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A

H

F

F

-

H

G

G

K

K

I

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N

I

I

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V

N

N

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x
N

A

A

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G

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I

N

N

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A

A

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A

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I

H

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K

V

A

L

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M

H

P

P

G

F

M

L

M

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A

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S

K

E

Q

R

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G

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N

I

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N

F

I

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S
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S

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S

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G

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A

I

L

A

A

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A

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A

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Y
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Y

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K

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A

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C

L

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E

A

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A

A

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K

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D

N

N

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N

A

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C

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P

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G

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x
I

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x
S

M
x
L

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x
V

K

E

E

M

L

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W

I

S

Q

A

D

P

P

E

E

R

Q

K

K

D

E

-

N

-

L

-

N

P

K

F

L

I

I

A

D

R

R

T

C

P

A

L

L

G

R

R

R

F

M

G

G

E

E

P

P

I

K

E

E

I

L

A

A

D

A

M

M

A

V

L

A

Y

F

L

L

A

C

S

F

P

P

A

A

S

A

D

S

L

Y

V

V

N

T

G

G

A

Q

V

I

M

I

M

Y

I

V

E

D

G

G

active site: G19 (= G22), S145 (= S147), Y158 (= Y160), K162 (= K164)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G18), S17 (= S20), R18 (≠ S21), G19 (= G22), I20 (= I23), S39 (≠ G42), R40 (= R43), C64 (≠ G67), D65 (= D68), L66 (= L69), N93 (≠ S95), I116 (≠ V118), S145 (= S147), Y158 (= Y160), K162 (= K164), P188 (= P190), I191 (≠ V193), T193 (= T195), S194 (≠ E196), L195 (≠ M197), V196 (≠ G198)

5yssB Crystal structure of aminocaproic acid cyclase in complex with NAD (+) (see paper)
37% identity, 96% coverage: 8:252/255 of query aligns to 1:252/255 of 5yssB

query
sites
5yssB

S

N

L

L

Q

T

D

G

K

K

T

T

A

A

L

L

V

V

T

T

G
|
G

A

S

S
x
T

S
|
S

G
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G

I
|
I

G

G

R

L

A

G

I

I

A

A

E

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V

V

F

L

A

A

D

Q

A

A

G

G

A

A

D

T

I

L

V

I

G

L

Q

N

G
|
G

R
x
F

-

G

D

D

L

V

D

D

R

A

L

A

T

K

D

D

L

A

G

V

A

A

Q

Q

I

Y

-

G

K

K

T

T

T

P

G

G

R

Y

Q

H

F

G

A

A

A

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I

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G

-

D

D

L
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L

A

S

D

D

P

E

D

A

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Q

T

I

Q

A

N

D

V

M

A

M

D

R

R

Y

A

A

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E

A

S

A

E

F

F

G

G

K

G

I

V

D

D

I

I

L

L

V

I

N

N

S
x
N

A

A

G
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G

I
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I

A

Q

V

H

T

V

G

S

P

P

V

I

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E

N

T

Y

F

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P

L

V

D

D

D

K

W

W

Q

N

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A

T

I

L

I

A

A

V

I

N

N

L

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S

A

S

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F

F

I

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S

T

K

R

A

L

V

A

M

L

P

P

G

G

M

M

M

R

Q

A

R

R

K

N

Q

W

G

G

K

R

I

I

N

N

I

I

S
x
A

S

S

Q

V

T

H

G

G

V

L

I

V

A

A

L

S

K

K

D

E

H

K

A

S

A

A

Y
|
Y

A

V

T

A

S

A

K
|
K

G

H

G

G

L

V

N

V

A

G

L

L

T

T

K

K

S

T

L

I

M

A

T

L

E

E

A

T

A

A

P

Q

H

T

N

E

V

I

Q

T

V

C

N

N

A

A

I

L

C

C

P
|
P

T
x
G

V

W

V
|
V

L

L

T
|
T

-

P

-

L

-

V

-

Q

E

Q

M

I

G

D

K

K

E

R

L

I

W

A

S

E

A

G

P

A

E

E

-

P

-

E

-

A

R

A

K

R

D

D

P

A

F

L

I

L

A

A

-

E

R

K

T

Q

P

P

L

S

G

R

R

E

F

F

G

V

E

T

P

P

I

E

E

Q

I

L

A

G

D

N

M

L

A

A

L

L

Y

F

L

L

A

C

S

S

P

D

A

G

S

A

D

A

L

Q

V

V

N

R

G

G

A

V

V

A

M

W

M

N

I

M

E

D

G

G

Y

W

binding nicotinamide-adenine-dinucleotide: G11 (= G18), T13 (≠ S20), S14 (= S21), G15 (= G22), I16 (= I23), G35 (= G42), F36 (≠ R43), L60 (= L69), N86 (≠ S95), G88 (= G97), I89 (= I98), A137 (≠ S146), Y151 (= Y160), K155 (= K164), P181 (= P190), G182 (≠ T191), V184 (= V193), T186 (= T195)

6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
36% identity, 96% coverage: 7:251/255 of query aligns to 1:240/244 of 6t77A

query
sites
6t77A

F

M

S

S

L

F

Q

E

D

G

K

K

T

I

A

A

L

L

V

V

T

T

G
|
G

A

A

S
|
S

S
x
R

G
|
G

I

I

G

G

R

R

A

A

I

I

A

A

E

E

V

T

F

L

A

V

D

A

A

R

G

G

A

A

D

K

I

V

V

I

G

G

Q

T

G

A

R

-

D

-

L

-

D

-

R

-

L
x
T

T

S

D

E

L

S

G

G

A

A

Q

Q

I

A

K

I

T

S

-

D

-

Y

T

L

G

G

R

A

Q

N

F

G

A

K

A

G

I

L

T

M

G
x
L

D
x
N

L
x
V

A

T

D

D

P

P

D

A

Q

S

T

I

Q

E

N

S

V

V

A

L

D

E

R

N

A

V

L

R

A

A

A

E

F

F

G

G

K

E

I

V

D

D

I

I

L

L

V

V

N

N

S

N

A
|
A

G
|
G

I
|
I

A

T

V

R

T

D

G

N

P

L

V

L

T

M

N

R

Y

M

D

K

L

D

D

D

D

E

W

W

Q

N

R

D

T

I

L

I

A

E

V

T

N

N

L

L

T

S

A

S

P

V

F

F

I

R

L

L

S

S

K

K

A

A

V

V

M

M

P

R

G

A

M

M

Q

K

R

K

K

R

Q

H

G

G

K

R

I

I

N

T

I

I

S

G

S
|
S

Q

V

T

V

G

G

V

T

I

M

A

G

L

N

K

A

D

G

H

Q

A

A

A

N

Y
|
Y

A

A

T

A

S

A

K

K

G

A

G

G

L

L

N

I

A

G

L

F

T

S

K

K

S

S

L

L

M

A

T

R

E

E

A

V

A

A

P

S

H

R

N

G

V

I

Q

T

V

V

N

N

A

V

I

V

C

A

P

P

T

G

V

F

V

I

L

E

T

T

E

D

M

M

G

T

K

R

E

A

L

L

W

-

S

-

A

T

P

D

E

E

R

Q

K

R

D

A

P

G

F

T

I

L

A

A

R

A

T

V

P

P

L

A

G

G

R

R

F

L

G

G

E

T

P

P

I

N

E

E

I

I

A

A

D

S

M

A

A

V

L

A

Y

F

L

L

A

A

S

S

P

D

A

E

S

A

D

S

L

Y

V

I

N

T

G

G

A

E

V

T

M

L

M

H

I

V

E

N

G

G

active site: G16 (= G22), S138 (= S147), Y151 (= Y160)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G18), S14 (= S20), R15 (≠ S21), T37 (≠ L48), L58 (≠ G67), N59 (≠ D68), V60 (≠ L69), A87 (= A96), G88 (= G97), I89 (= I98)

6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
31% identity, 97% coverage: 9:255/255 of query aligns to 3:248/248 of 6ixmC

query
sites
6ixmC

L

L

Q

D

D

N

K

K

T

V

A

A

L

L

V

V

T

T

G
|
G

A

A

S

G

S
|
S

G
|
G

I
|
I

G

G

R

L

A

A

I

V

A

A

E

H

V

S

F

Y

A

A

D

K

A

E

G

G

A

A

D

K

I

V

V

I

G

V

Q

S

G
x
D

R
x
I

D

N

L

E

D

D

R

H

L

G

T

N

D

K

L

A

G

V

A

E

Q

D

I

I

K

K

T

A

T

Q

G

G

R

G

Q

E

F

A

A

S

A

F

I

V

T

K

G
x
A

D
|
D

L
x
T

A

S

D

N

P

P

D

E

Q

E

T

V

Q

E

N

A

V

L

A

V

D

K

R

R

A

T

L

V

A

E

A

I

F

Y

G

G

K

R

I

L

D

D

I

I

L

A

V

C

N

N

S
x
N

A
|
A

G

G

I

I

A

G

V

G

T

E

G

Q

P

A

V

L

T

A

-

G

N

D

Y

Y

D

G

L

L

D

D

D

S

W

W

Q

R

R

K

T

V

L

L

A

S

V

I

N

N

L

L

T

D

A

G

P

V

F

F

I

Y

L

G

S

C

K

K

A

Y

V

E

M

L

P

E

G

Q

M

M

M

E

Q

K

R

N

K

G

Q

G

G

G

K

V

I

I

I

V

N

N

I
x
M

S

A

S
|
S

Q

I

T

H

G

G

V

I

I

V

A

A

L

A

K

P

D

L

H

S

A

S

A

A

Y
|
Y

A

T

T

S

S

A

K
|
K

G

H

G

A

L

V

N

V

A

G

L

L

T

T

K

K

S

N

L

I

M

G

T

A

E

E

A

Y

A

G

P

Q

H

K

N

N

V

I

Q

R

V

C

N

N

A

A

I

V

C

G

P
|
P

T
x
A

V
x
Y

V
x
I

L

E

T

T

E

P

M
x
L

G

L

K

E

E

S

L

L

W

-

S

-

A

T

P

K

E

E

R

M

K

K

D

E

P

A

F

L

I

I

A

S

R

K

T

H

P

P

L

M

G

G

R

R

F

L

G

G

E

K

P

P

I

E

E

E

I

V

A

A

D

E

M

L

A

V

L

L

Y

F

L

L

A

S

S

S

P

E

A

K

S

S

D

S

L

F

V

M

N

T

G

G

A

G

V

Y

M

Y

M

L

I

V

E

D

G

G

Y

Y

S

T

S

A

I

V

active site: G16 (= G22), S142 (= S147), Y155 (= Y160), K159 (= K164)
binding nicotinamide-adenine-dinucleotide: G12 (= G18), S15 (= S21), G16 (= G22), I17 (= I23), D36 (≠ G42), I37 (≠ R43), A61 (≠ G67), D62 (= D68), T63 (≠ L69), N89 (≠ S95), A90 (= A96), M140 (≠ I145), S142 (= S147), Y155 (= Y160), K159 (= K164), P185 (= P190), A186 (≠ T191), Y187 (≠ V192), I188 (≠ V193), L192 (≠ M197)

4nbuB Crystal structure of fabg from bacillus sp (see paper)
38% identity, 95% coverage: 9:251/255 of query aligns to 5:241/244 of 4nbuB

query
sites
4nbuB

L

L

Q

Q

D

D

K

K

T

V

A

A

L

I

V

I

T

T

G
|
G

A

A

S

A

S
x
N

G
|
G

I
|
I

G

G

R

L

A

E

I

A

A

A

E

R

V

V

F

F

A

M

D

K

A

E

G

G

A

A

D

K

I

V

V

V

G

I

Q

A

G

-

R

-

D
|
D

L
x
F

D

N

R

E

L

A

T

A

D

G

L

K

G

E

A

A

Q

V

I

E

K

A

T

N

T

P

G

G

R

V

Q

V

F

F

A

-

I

I

T

R

G
x
V

D
|
D

L
x
V

A

S

D

D

P

R

D

E

Q

S

T

V

Q

H

N

R

V

L

A

V

D

E

R

N

A

V

L

A

A

E

A

R

F

F

G

G

K

K

I

I

D

D

I

I

L

L

V

I

N

N

S
x
N

A
|
A

G
|
G

I
|
I

A

T

V

R

T
x
D

G

S

P

M

V

L

T

S

N
x
K

Y

M

D

T

L

V

D

D

D

Q

W

F

Q

Q

R

Q

T

V

L

I

A

N

V

V

N
|
N

L

L

T

T

A

G

P

V

F

F

I

H

L

C

S

T

K

Q

A

A

V

V

M

L

P

P

G

Y

M

M

M

A

Q

E

R

Q

K

G

Q

K

G

G

K

K

I

I

N

N

I
x
T

S

S

S
|
S

Q

V

T

T

G

G

V

T

I

Y

A

G

L
x
N

K
x
V

D

G

H
x
Q

A

T

A

N

Y
|
Y

A

A

T

A

S

A

K
|
K

G

A

G

G

L

V

N

I

A

G

L

M

T

T

K

K

S

T

L

W

M

A

T

K

E

E

A

L

A

A

P

R

H

K

N

G

V

I

Q

N

V

V

N

N

A

A

I

V

C

A

P
|
P

T

G

V
x
F

V
x
T

L

E

T
|
T

E

A

M
|
M

G

V

K

A

E

E

L

V

W

-

S

-

A

-

P

P

E

E

R

K

K

V

-

I

D

E

P
x
K

F

M

I

K

A

A

R

Q

T

V

P

P

L

M

G

G

R

R

F

L

G

G

E

K

P

P

I

E

E

D

I

I

A

A

D

N

M

A

A

Y

L

L

Y

F

L

L

A

A

S

S

P

H

A

E

S

S

D

D

L

Y

V

V

N

N

G

G

A

H

V

V

M

L

M

H

I

V

E

D

G

G

active site: G18 (= G22), N111 (= N119), S139 (= S147), Q149 (≠ H157), Y152 (= Y160), K156 (= K164)
binding acetoacetyl-coenzyme a: D93 (≠ T101), K98 (≠ N106), S139 (= S147), N146 (≠ L154), V147 (≠ K155), Q149 (≠ H157), Y152 (= Y160), F184 (≠ V192), M189 (= M197), K200 (≠ P210)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G18), N17 (≠ S21), G18 (= G22), I19 (= I23), D38 (= D44), F39 (≠ L45), V59 (≠ G67), D60 (= D68), V61 (≠ L69), N87 (≠ S95), A88 (= A96), G89 (= G97), I90 (= I98), T137 (≠ I145), S139 (= S147), Y152 (= Y160), K156 (= K164), P182 (= P190), F184 (≠ V192), T185 (≠ V193), T187 (= T195), M189 (= M197)

5itvD Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
33% identity, 97% coverage: 7:254/255 of query aligns to 3:226/227 of 5itvD

query
sites
5itvD

F

M

S

N

L

L

Q

T

D

D

K

K

T

T

A

V

L

L

V

I

T

T

G
|
G

A

G

S

A

S
|
S

G
|
G

I
|
I

G

G

R

Y

A

A

I

A

A

V

E

Q

V

A

F

F

A

L

D

G

A

Q

G

Q

A

A

D

N

I

V

V

V

G

V

Q

-

G

-

R

A

D
|
D

L
x
I

D

D

R

E

L

A

T

Q

D

G

L

E

G

A

A

M

Q

V

I

R

K

K

T

E

T

N

G

N

R

D

Q

R

F

L

A

H

A

F

I

V

T

Q

G
x
T

D
|
D

L
x
I

A

T

D

D

P

E

D

A

Q

A

T

C

Q

Q

N

H

V

A

A

V

D

E

R

S

A

A

L

V

A

H

A

T

F

F

G

G

K

G

I

L

D

D

I

V

L

L

V

I

N

N

S
x
N

A

A

G

G

I

I

A

E

V

I

T

V

G

A

P

P

V

I

T

H

N

E

Y

M

D

E

L

L

D

S

D

D

W

W

Q

N

R

K

T

V

L

L

A

Q

V

V

N

N

L

L

T

T

A

G

P

M

F

F

I

L

L

M

S

S

K

K

A

H

V

A

M

L

P

K

G

H

M

M

M

L

Q

A

R

A

K

G

Q

K

G

G

K

N

I

I

N

N

I
x
T

S

C

S
|
S

Q

V

T

G

G

G

V

L

I

V

A

A

L

W

K

P

D

D

H

I

A

P

A

A

Y
|
Y

A

N

T

A

S

S

K
|
K

G

G

L

V

N

L

A

Q

L

L

T

T

K

K

S

S

L

M

M

A

T

V

E

D

A

Y

A

A

P

K

H

H

N

Q

V

I

Q

R

V

V

N

N

A

C

I

V

C

C

P
|
P

T
x
G

V

I

V
x
I

L

D

T

T

E

L

M

-

G

-

K

-

E

-

L

-

W

-

S

-

A

-

P

-

E

-

R

-

K

-

D

-

P

-

F

-

I

-

A

-

R

-

T

-

P

-

L

-

G

-

R

R

F

L

G

G

E

K

P

P

I

E

E

E

I

I

A

A

D

N

M

V

A

M

L

L

Y

F

L

L

A

A

S

S

P

D

A

L

S

S

D

S

L

Y

V

M

N

T

G

G

A

S

V

A

M

I

M

T

I

A

E

D

G

G

Y

Y

S

T

S

A

active site: G18 (= G22), S141 (= S147), Y154 (= Y160), K158 (= K164)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G18), S17 (= S21), G18 (= G22), I19 (= I23), D38 (= D44), I39 (≠ L45), T61 (≠ G67), D62 (= D68), I63 (≠ L69), N89 (≠ S95), T139 (≠ I145), S141 (= S147), Y154 (= Y160), K158 (= K164), P184 (= P190), G185 (≠ T191), I187 (≠ V193)

Q9ZW19 Tropinone reductase homolog At2g29360; EC 1.1.1.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 97% coverage: 6:253/255 of query aligns to 13:261/271 of Q9ZW19

query
sites
Q9ZW19

R

R

F

W

S

S

L

L

Q

V

D

G

K

M

T

T

A

A

L

L

V

V

T

T

G

G

A

G

S

S

S

K

G

G

I

I

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S209 (≠ L201) mutation to Y: Loss of activity with quinuclidinone and decreased activity with cyclohexanones.

1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
32% identity, 97% coverage: 7:254/255 of query aligns to 2:251/252 of 1vl8B

query
sites
1vl8B

F

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active site: G17 (= G22), S143 (= S147), I154 (≠ H157), Y157 (= Y160), K161 (= K164)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G18), R16 (≠ S21), G17 (= G22), L18 (≠ I23), S37 (≠ G42), R38 (= R43), C63 (≠ G67), D64 (= D68), V65 (≠ L69), A91 (≠ S95), A92 (= A96), G93 (= G97), I94 (= I98), V114 (= V118), I141 (= I145), S143 (= S147), Y157 (= Y160), K161 (= K164), P187 (= P190), G188 (≠ T191), Y190 (≠ V193), T192 (= T195), M194 (= M197), T195 (≠ G198)

Query Sequence

>3607131 FitnessBrowser__Dino:3607131
MPWQDRFSLQDKTALVTGASSGIGRAIAEVFADAGADIVGQGRDLDRLTDLGAQIKTTGR
QFAAITGDLADPDQTQNVADRALAAFGKIDILVNSAGIAVTGPVTNYDLDDWQRTLAVNL
TAPFILSKAVMPGMMQRKQGKIINISSQTGVIALKDHAAYATSKGGLNALTKSLMTEAAP
HNVQVNAICPTVVLTEMGKELWSAPERKDPFIARTPLGRFGEPIEIADMALYLASPASDL
VNGAVMMIEGGYSSI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory