SitesBLAST
Comparing 3607199 FitnessBrowser__Dino:3607199 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
35% identity, 95% coverage: 1:238/250 of query aligns to 5:242/244 of 4nbuB
- active site: G18 (= G14), N111 (= N107), S139 (= S134), Q149 (≠ R144), Y152 (= Y147), K156 (= K151)
- binding acetoacetyl-coenzyme a: D93 (≠ F89), K98 (≠ E94), S139 (= S134), N146 (≠ S141), V147 (≠ T142), Q149 (≠ R144), Y152 (= Y147), F184 (≠ P179), M189 (≠ L184), K200 (≠ A196)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G10), N17 (≠ R13), G18 (= G14), I19 (= I15), D38 (= D34), F39 (≠ R35), V59 (≠ C55), D60 (= D56), V61 (= V57), N87 (= N83), A88 (= A84), G89 (= G85), I90 (≠ V86), T137 (≠ I132), S139 (= S134), Y152 (= Y147), K156 (= K151), P182 (= P177), F184 (≠ P179), T185 (≠ V180), T187 (= T182), M189 (≠ L184)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
37% identity, 94% coverage: 3:238/250 of query aligns to 4:244/246 of 3osuA
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
37% identity, 96% coverage: 1:240/250 of query aligns to 5:254/255 of 5itvA
- active site: G18 (= G14), S141 (= S134), Y154 (= Y147), K158 (= K151)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G10), S17 (≠ R13), G18 (= G14), I19 (= I15), D38 (= D34), I39 (≠ R35), T61 (≠ C55), I63 (≠ V57), N89 (= N83), G91 (= G85), T139 (≠ I132), S141 (= S134), Y154 (= Y147), K158 (= K151), P184 (= P177), G185 (= G178), I186 (vs. gap), I187 (vs. gap)
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
36% identity, 94% coverage: 3:238/250 of query aligns to 1:237/239 of 3sj7A
- active site: G12 (= G14), S138 (= S134), Q148 (≠ R144), Y151 (= Y147), K155 (= K151)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), S10 (≠ A12), R11 (= R13), I13 (= I15), N31 (≠ I33), Y32 (≠ D34), A33 (≠ R35), G34 (≠ D36), S35 (≠ G37), A58 (≠ C55), N59 (≠ D56), V60 (= V57), N86 (= N83), A87 (= A84), T109 (= T106), S138 (= S134), Y151 (= Y147), K155 (= K151), P181 (= P177), G182 (= G178)
5itvD Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
36% identity, 96% coverage: 1:240/250 of query aligns to 5:226/227 of 5itvD
- active site: G18 (= G14), S141 (= S134), Y154 (= Y147), K158 (= K151)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G10), S17 (≠ R13), G18 (= G14), I19 (= I15), D38 (= D34), I39 (≠ R35), T61 (≠ C55), D62 (= D56), I63 (≠ V57), N89 (= N83), T139 (≠ I132), S141 (= S134), Y154 (= Y147), K158 (= K151), P184 (= P177), G185 (= G178), I187 (= I194)
6vspB Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
37% identity, 96% coverage: 3:241/250 of query aligns to 7:246/252 of 6vspB
6vspA Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
37% identity, 96% coverage: 3:241/250 of query aligns to 5:244/251 of 6vspA
- active site: G16 (= G14), S138 (= S134), Y151 (= Y147)
- binding nicotinamide-adenine-dinucleotide: G12 (= G10), N15 (≠ R13), G16 (= G14), M17 (≠ I15), D36 (= D34), W37 (≠ R35), W37 (≠ R35), A38 (≠ D36), I59 (≠ C55), D60 (= D56), V61 (= V57), N87 (= N83), A88 (= A84), G89 (= G85), V90 (= V86), V110 (≠ T106), T136 (≠ I132), S138 (= S134), Y151 (= Y147), K155 (= K151), P181 (= P177), S182 (≠ G178), L183 (≠ P179), V184 (= V180), T186 (= T182), N187 (≠ K183), M188 (≠ L184), T189 (≠ A185)
6xewA Structure of serratia marcescens 2,3-butanediol dehydrogenase (see paper)
37% identity, 96% coverage: 3:241/250 of query aligns to 5:244/251 of 6xewA
- active site: G16 (= G14), S138 (= S134), Y151 (= Y147)
- binding r,3-hydroxybutan-2-one: S138 (= S134), S140 (= S136), Y151 (= Y147)
- binding s,3-hydroxybutan-2-one: S138 (= S134), Y151 (= Y147), S182 (≠ G178)
- binding nicotinamide-adenine-dinucleotide: G12 (= G10), N15 (≠ R13), G16 (= G14), M17 (≠ I15), D36 (= D34), W37 (≠ R35), W37 (≠ R35), A38 (≠ D36), I59 (≠ C55), D60 (= D56), V61 (= V57), N87 (= N83), A88 (= A84), G89 (= G85), V110 (≠ T106), T136 (≠ I132), S138 (= S134), Y151 (= Y147), K155 (= K151), S182 (≠ G178), L183 (≠ P179), V184 (= V180), T186 (= T182), N187 (≠ K183), M188 (≠ L184), T189 (≠ A185)
H9XP47 Meso-2,3-butanediol dehydrogenase; BDH; meso-2,3-BDH; (R,S)-butane-2,3-diol dehydrogenase; NAD(H)-dependent meso-2,3-BDH; SmBdh; EC 1.1.1.- from Serratia marcescens (see paper)
37% identity, 96% coverage: 3:241/250 of query aligns to 5:244/251 of H9XP47
- N15 (≠ R13) binding
- M17 (≠ I15) binding
- D36 (= D34) binding
- D60 (= D56) binding
- V61 (= V57) binding
- N87 (= N83) binding
- S138 (= S134) binding ; binding
- V139 (≠ I135) mutation to Q: Retains 50% of activity with acetoin as substrate; when associated with A-247.
- S140 (= S136) binding
- Y151 (= Y147) binding ; binding ; binding
- K155 (= K151) binding
- V184 (= V180) binding
- T186 (= T182) binding
- RDK 197:199 (≠ IDA 194:196) mutation to SEAAGKPLGYGTET: Mimics longer alpha6 helix. Retains 3% of activity with acetoin as substrate.
Sites not aligning to the query:
- 247 Q→A: Retains 10% of activity with acetoin as substrate. Retains 50% of activity with acetoin as substrate; when associated with Q-139.
2cfcA Structural basis for stereo selectivity in the (r)- and (s)-hydroxypropylethane thiosulfonate dehydrogenases (see paper)
38% identity, 95% coverage: 3:240/250 of query aligns to 2:249/250 of 2cfcA
- active site: G13 (= G14), S142 (= S134), Y155 (= Y147), K159 (= K151)
- binding (2-[2-ketopropylthio]ethanesulfonate: F149 (≠ S141), R152 (= R144), Y155 (= Y147), W195 (≠ A187), R196 (≠ V188)
- binding nicotinamide-adenine-dinucleotide: G9 (= G10), S12 (≠ R13), G13 (= G14), N14 (≠ I15), D33 (= D34), L34 (≠ R35), A59 (≠ C55), D60 (= D56), V61 (= V57), N87 (= N83), A88 (= A84), G89 (= G85), I140 (= I132), P185 (= P177), G186 (= G178), M187 (≠ P179), I188 (≠ V180), T190 (= T182), P191 (≠ K183), M192 (≠ L184), T193 (≠ A185)
Q56840 2-(R)-hydroxypropyl-CoM dehydrogenase; R-HPCDH; 2-[(R)-2-hydroxypropylthio]ethanesulfonate dehydrogenase; Aliphatic epoxide carboxylation component III; Epoxide carboxylase component III; RHPCDH1; EC 1.1.1.268 from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) (see 4 papers)
38% identity, 95% coverage: 3:240/250 of query aligns to 2:249/250 of Q56840
- SGN 12:14 (≠ RGI 13:15) binding
- D33 (= D34) binding
- DV 60:61 (= DV 56:57) binding
- N87 (= N83) binding
- S142 (= S134) mutation to A: Retains weak activity. 120-fold decrease in kcat.; mutation to C: Loss of activity.
- R152 (= R144) binding ; mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- Y155 (= Y147) mutation Y->E,F: Loss of activity.
- K159 (= K151) mutation to A: Loss of activity.
- R179 (= R171) mutation to A: Loss of activity.
- IETPM 188:192 (≠ VRTKL 180:184) binding
- WR 195:196 (≠ AV 187:188) binding
- R196 (≠ V188) mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- R203 (≠ I194) mutation to A: Slight decrease in catalytic efficiency.
- R209 (≠ A200) mutation to A: Does not affect catalytic efficiency.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
5ha5D Crystal structure of an NAD-bound oxidoreductase from brucella ovis
38% identity, 96% coverage: 1:240/250 of query aligns to 4:243/244 of 5ha5D
- active site: G17 (= G14), S142 (= S134), Y155 (= Y147), K159 (= K151)
- binding nicotinamide-adenine-dinucleotide: G13 (= G10), R16 (= R13), G17 (= G14), L18 (≠ I15), D37 (= D34), I38 (≠ R35), L62 (≠ C55), D63 (= D56), V64 (= V57), N90 (= N83), A91 (= A84), S142 (= S134), Y155 (= Y147), K159 (= K151), G186 (= G178), M188 (≠ V180), S190 (≠ T182)
4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
36% identity, 96% coverage: 1:240/250 of query aligns to 8:252/258 of 4wecA
- active site: G21 (= G14), S143 (= S134), Q154 (≠ R144), Y157 (= Y147), K161 (= K151)
- binding nicotinamide-adenine-dinucleotide: G17 (= G10), A19 (= A12), S20 (≠ R13), G21 (= G14), I22 (= I15), D41 (= D34), I42 (≠ R35), V61 (≠ C55), D62 (= D56), V63 (= V57), N89 (= N83), T141 (≠ I132), Y157 (= Y147), K161 (= K151), P187 (= P177), P189 (= P179), V190 (= V180)
1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
34% identity, 96% coverage: 1:241/250 of query aligns to 4:252/252 of 1vl8B
- active site: G17 (= G14), S143 (= S134), I154 (≠ V145), Y157 (= Y147), K161 (= K151)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G10), R16 (= R13), G17 (= G14), L18 (≠ I15), S37 (≠ D34), R38 (= R35), C63 (= C55), D64 (= D56), V65 (= V57), A91 (≠ N83), A92 (= A84), G93 (= G85), I94 (≠ V86), V114 (≠ T106), I141 (= I132), S143 (= S134), Y157 (= Y147), K161 (= K151), P187 (= P177), G188 (= G178), Y190 (≠ V180), T192 (= T182), M194 (≠ L184), T195 (≠ A185)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
35% identity, 96% coverage: 1:240/250 of query aligns to 3:247/248 of 6ixmC
- active site: G16 (= G14), S142 (= S134), Y155 (= Y147), K159 (= K151)
- binding nicotinamide-adenine-dinucleotide: G12 (= G10), S15 (≠ R13), G16 (= G14), I17 (= I15), D36 (= D34), I37 (≠ R35), A61 (≠ C55), D62 (= D56), T63 (≠ V57), N89 (= N83), A90 (= A84), M140 (≠ I132), S142 (= S134), Y155 (= Y147), K159 (= K151), P185 (= P177), A186 (≠ G178), Y187 (≠ P179), I188 (≠ V180), L192 (= L184)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
35% identity, 95% coverage: 1:238/250 of query aligns to 3:245/247 of 4jroC
- active site: G16 (= G14), S142 (= S134), Q152 (≠ R144), Y155 (= Y147), K159 (= K151)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G10), S14 (≠ A12), R15 (= R13), G16 (= G14), I17 (= I15), N35 (≠ I33), Y36 (≠ D34), N37 (≠ R35), G38 (≠ D36), S39 (≠ G37), N63 (≠ D56), V64 (= V57), N90 (= N83), A91 (= A84), I93 (≠ V86), I113 (≠ T106), S142 (= S134), Y155 (= Y147), K159 (= K151), P185 (= P177), I188 (≠ V180), T190 (= T182)
2dtxA Structure of thermoplasma acidophilum aldohexose dehydrogenase (aldt) in complex with d-mannose (see paper)
34% identity, 95% coverage: 1:238/250 of query aligns to 5:245/255 of 2dtxA
2dteA Structure of thermoplasma acidophilum aldohexose dehydrogenase (aldt) in complex with nadh (see paper)
34% identity, 95% coverage: 1:238/250 of query aligns to 5:245/255 of 2dteA
- active site: G18 (= G14), S132 (= S134), Y145 (= Y147), S148 (= S150), K149 (= K151)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G10), S16 (≠ A12), M17 (≠ R13), G18 (= G14), I19 (= I15), S38 (≠ D34), I39 (≠ R35), C52 (= C55), D53 (= D56), V54 (= V57), N80 (= N83), A81 (= A84), I130 (= I132), S132 (= S134), Y145 (= Y147), K149 (= K151), P174 (= P177), A175 (≠ G178), T176 (≠ P179), I177 (≠ V180), T179 (= T182), P180 (≠ K183), L181 (= L184), V182 (≠ A185)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
35% identity, 95% coverage: 1:238/250 of query aligns to 6:245/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G10), R18 (= R13), G19 (= G14), I20 (= I15), D39 (= D34), R40 (= R35), C63 (= C55), I65 (≠ V57), N91 (= N83), G93 (= G85), I94 (≠ V86), V114 (≠ T106), Y155 (= Y147), K159 (= K151), I188 (≠ V180), T190 (= T182), T193 (≠ A185)
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
35% identity, 96% coverage: 1:240/250 of query aligns to 5:260/261 of 6zzsD
- active site: G18 (= G14), S143 (= S134), Y156 (= Y147)
- binding nicotinamide-adenine-dinucleotide: G14 (= G10), S17 (≠ R13), I19 (= I15), D38 (= D34), M39 (≠ R35), D64 (= D56), V65 (= V57), N91 (= N83), A92 (= A84), G93 (= G85), M141 (≠ I132), A142 (= A133), S143 (= S134), Y156 (= Y147), K160 (= K151), P186 (= P177), G187 (= G178), V189 (= V180), T191 (= T182), L193 (= L184)
- binding 3-oxidanylidenepentanoic acid: Q95 (≠ A87), S143 (= S134), N145 (≠ S136), K153 (≠ R144), Y156 (= Y147), Q197 (vs. gap)
Query Sequence
>3607199 FitnessBrowser__Dino:3607199
MTSKTAVVTGAARGIGLATTKLLLERGWKVAMIDRDGPALAEALQGLDGVHGIDCDVSDP
EAVEGMIAETLREFGQIDALVNNAGVADFGPIEETSFARWKTVMETNLDGPFLCVQAATP
ALKATKGAVVNIASISGLRASTLRVAYGTSKAAVIQLTLQQAVELGEHGIRANCVCPGPV
RTKLAMAVHSQEIIDAYHDAIPLNRYGSEQEIAEAILFLCSERASFITGQVLAADGGFEA
TGIGLPALRG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory