SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3607281 FitnessBrowser__Dino:3607281 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
38% identity, 95% coverage: 11:218/219 of query aligns to 545:748/758 of O14289

query
sites
O14289

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Sites not aligning to the query:

486 modified: Phosphoserine
488 modified: Phosphoserine

P9WK95 3-isopropylmalate dehydratase small subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
37% identity, 87% coverage: 9:199/219 of query aligns to 7:192/198 of P9WK95

query
sites
P9WK95

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K154 (≠ T161) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

2pkpA Crystal structure of 3-isopropylmalate dehydratase (leud)from methhanocaldococcus jannaschii dsm2661 (mj1271) (see paper)
30% identity, 69% coverage: 21:172/219 of query aligns to 14:163/167 of 2pkpA

query
sites
2pkpA

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binding zinc ion: C63 (≠ S81)

Sites not aligning to the query:

binding zinc ion: 13

Q58667 Methanogen homoaconitase small subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
32% identity, 69% coverage: 21:172/219 of query aligns to 14:163/170 of Q58667

query
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Q58667

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R26 (≠ S33) mutation R->K,V: Allows the recognition of substrates of IPMI enzymes since it becomes able to efficiently catalyze citraconate hydration and 3-isopropylmalate dehydration. Largely decreases substrate affinity for cis-homo(1-3)-aconitate.
T27 (= T34) mutation to A: Largely decreases substrate affinity for cis-homo(1-3)-aconitate while slightly increases activity on these substrates, and also decreases substrate affinity for maleate. Gains the ability to hydrate citraconate, an IPMI substrate.

P21399 Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1; EC 4.2.1.3 from Homo sapiens (Human) (see 2 papers)
39% identity, 35% coverage: 61:137/219 of query aligns to 755:834/889 of P21399

query
sites
P21399

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S778 (= S83) mutation to A: No effect on iron-regulated RNA binding. Loss of aconitase activity.
R780 (= R85) mutation to Q: Nearly abolishes RNA binding.

Sites not aligning to the query:

300 C→S: No effect on aconitase activity or on RNA binding.
318 T → M: in dbSNP:rs150373174
437 C→S: Loss of aconitase activity. Leads to constitutive RNA binding, irrespective of iron levels.
503 C→S: Loss of aconitase activity. Leads to constitutive RNA binding, irrespective of iron levels.
506 C→S: Loss of aconitase activity. Leads to constitutive RNA binding, irrespective of iron levels.
536 R→Q: Strongly reduced RNA binding.
541 R→Q: Strongly reduced RNA binding.
699 R→K: No effect on RNA binding.

2b3xA Structure of an orthorhombic crystal form of human cytosolic aconitase (irp1) (see paper)
39% identity, 35% coverage: 61:137/219 of query aligns to 754:833/888 of 2b3xA

query
sites
2b3xA

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active site: S777 (= S83), R779 (= R85)

Sites not aligning to the query:

active site: 124, 125, 204, 535
binding iron/sulfur cluster: 175, 206, 436, 502, 505, 506, 534

Q9SIB9 Aconitate hydratase 3, mitochondrial; Aconitase 3; mACO1; Citrate hydro-lyase 3; EC 4.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
37% identity, 39% coverage: 61:146/219 of query aligns to 852:940/990 of Q9SIB9

query
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Q9SIB9

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Sites not aligning to the query:

91 modified: Phosphoserine

3snpA Crystal structure analysis of iron regulatory protein 1 in complex with ferritin h ire RNA (see paper)
38% identity, 35% coverage: 61:137/219 of query aligns to 716:795/850 of 3snpA

query
sites
3snpA

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active site: S739 (= S83), R741 (= R85)
binding : R741 (= R85)

Sites not aligning to the query:

active site: 124, 125, 186, 505
binding : 125, 126, 188, 243, 250, 279, 283, 352, 357, 360, 419, 420, 421, 504, 505, 520, 642, 643, 644, 645, 646, 649, 665, 669, 671, 674

P09339 Aconitate hydratase A; ACN; Aconitase; Aconitate/2-methylaconitate hydratase; Iron-responsive protein-like; IRP-like; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.- from Bacillus subtilis (strain 168) (see 2 papers)
34% identity, 37% coverage: 66:147/219 of query aligns to 775:854/909 of P09339

query
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P09339

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H

A

R

A

S

N

N

A

L

V

V

N

F

N

M

G

G

L

V

C

L

P

P

V

L

Q

Q

V

F

R

K

P

Q

E

G

D

E

G

N

A

A

A

D

L

T

L

L

R

-

G

G

L

L

E

T

R

-

G

G

A

K

R

E

Q

V

M

I

T

E

V

V

D

D

L

V

E

D

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
450 C→S: Loss of aconitase activity. It is glutamate auxotroph and accumulates citrate. Exhibits overexpression of the citB promoter and accumulates high levels of inactive aconitase.
741 R→E: Same aconitase activity compared to the wild-type. It is glutamate prototroph and accumulates citrate. Exhibits overexpression of the citB promoter and accumulates high levels of active aconitase.

A0QX20 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
34% identity, 28% coverage: 72:133/219 of query aligns to 811:872/943 of A0QX20

query
sites
A0QX20

I

V

L

V

V

L

T

G

G

G

R

K

N

E

F

Y

G

G

S

S

G

G

S

S

R

R

E

D

A

W

A

A

V

A

Y

K

A

G

L

T

M

V

D

L

F

L

G

G

I

V

R

K

A

A

V

V

V

I

A

T

P

E

S

S

F

F

G

E

D

R

I

I

F

H

A

R

A

S

N

N

A

L

V

I

N

G

N

M

G

G

L

V

C

I

P

P

V

L

Q

Q

V

F

R

P

P

A

E

G

D

E

G

S

A

A

L

S

L

L

R

K

Sites not aligning to the query:

394 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Query Sequence

>3607281 FitnessBrowser__Dino:3607281
MSARGWTRHTGRAAALPMEAVDTDQLIPARFMSTPRAEGYGRYLLHDLRFCDGCVPRPEF
ALNRVENAGATILVTGRNFGSGSSREAAVYALMDFGIRAVVAPSFGDIFAANAVNNGLCP
VQVRPEDGAALLRGLERGARQMTVDLEACALRGPLGTVGFTLDPVWRRKLIEGLDDIDLT
RAYGAEIAAFRAADAAARPWAALDMTEKTTGRGRPERSA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory