SitesBLAST

C9 (≠ F9) mutation to S: No effect.
C20 (= C20) mutation to S: Loss of oligomerization; no alteration of water permeability.
T183 (= T182) mutation to C: No effect.
R189 (= R188) mutation R->V,S: Loss of function.

2o9eA Crystal structure of aqpz mutant t183c complexed with mercury (see paper)
64% identity, 91% coverage: 1:208/228 of query aligns to 3:211/232 of 2o9eA

query
sites
2o9eA

M

M

F

F

K

R

R

K

T

L

L

A

A

A

E

E

F

S

I

F

G

G

T

T

Y

F

W

W

L

L

V

V

F

F

G

G

C

S

G

G

A

S

A

A

L

V

L

L

A

A

G

G

V

F

P

P

E

E

V

L

G

G

I

I

G

G

W

F

V

A

G

G

V

V

S

A

L

L

A

A

F

F

G

G

L

L

S

T

V

V

L

L

T

T

M

M

A

A

Y

F

A

A

V

V

G

G

G

H

I

I

S

S

G

G

H

H

F

F

N

N

P

P

A

A

V

V

T

T

L

I

G

G

L

L

T

W

I

A

A

G

G

G

R

R

F

F

E

P

A

A

K

K

D

E

I

V

P

V

P

G

Y

Y

W

V

I

I

A

A

Q

Q

L

V

G

G

A

I

A

V

A

A

G

A

T

L

L

L

F

Y

V

L

I

I

M

A

S

S

G

G

Q

K

A

T

E

G

F

F

A

D

S

A

V

A

G

A

S

S

-

G

F

F

A

A

S

S

N

N

G

G

Y

Y

G

G

V

E

A

H

S

S

P

P

G

G

F

Y

G

S

M

M

M

L

S

S

G

A

M

L

I

V

A

V

E
|
E

V

L

V

V

L

L

T

S

A

A

V

G

F

F

V

L

I

L

V

V

I

I

L

H

G

G

A

A

T

T

S

D

H

K

M

F

V

A

P

P

E

A

G

G

F

F

A

A

P

P

I

I

A

A

I

I

G

G

L

L

C

A

L

L

T

T

L

L

I

I

H

H

L

L

I

I

S
|
S

I

I

P

P

I

V

T

T

N

N

T

C

S

S

V

V

N

N

P

P

A

A

R

R

S

S

T

T

A

A

M

V

A

A

I

I

Y

F

A

Q

D

G

G

G

P

W

A

A

L

L

G

E

Q

Q

L

L

W

W

L

F

F

F

W

W

binding mercury (ii) ion: E140 (= E137), S179 (= S176)

3nkaA Crystal structure of aqpz h174g,t183f (see paper)
64% identity, 91% coverage: 1:208/228 of query aligns to 3:211/230 of 3nkaA

query
sites
3nkaA

M

M

F

F

K

R

R

K

T

L

L

A

A

A

E

E

F

C

I

F

G

G

T

T

Y

F

W

W

L

L

V

V

F

F

G

G

C

C

G

G

A

S

A
|
A

L

V

L

L

A

A

G

G

V

F

P

P

E

E

V

L

G

G

I

I

G

G

W

F

V

A

G

G

V
|
V

S

A

L

L

A

A

F

F

G

G

L

L

S

T

V

V

L

L

T

T

M

M

A

A

Y

F

A

A

V

V

G

G

G

H

I

I

S

S

G

G

H

H

F

F

N

N

P

P

A

A

V

V

T

T

L

I

G

G

L

L

T

W

I

A

A

G

G

G

R

R

F

F

E

P

A

A

K

K

D

E

I

V

P

V

P

G

Y

Y

W

V

I

I

A

A

Q

Q

L

V

G

G

A

I

A

V

A

A

G

A

T

L

L

L

F

Y

V

L

I

I

M

A

S

S

G

G

Q

K

A

T

E

G

F

F

A

D

S

A

V

A

G

A

S

S

-

G

F

F

A

A

S

S

N

N

G

G

Y

Y

G

G

V

E

A

H

S

S

P

P

G

G

F

Y

G

S

M

M

M

L

S

S

G

A

M

L

I

V

A

V

E

E

V

L

V

V

L

L

T

S

A

A

V

G

F

F

V

L

I

L

V

V

I

I

L

H

G

G

A

A

T

T

S

D

H

K

M

F

V

A

P

P

E

A

G

G

F

F

A

A

P

P

I

I

A

A

I

I

G

G

L

L

C

A

L

L

T

T

L

L

I

I

H

G

L

L

I

I

S

S

I
|
I

P

P

I

V

T

T

N
|
N

T

F

S

S

V

V

N

N

P

P

A

A

R

R

S

S

T

T

A

A

M

V

A

A

I

I

Y

F

A

Q

D

G

G

G

P

W

A

A

L

L

G

E

Q

Q

L

L

W

W

L

F

F

F

W

W

binding glycerol: A25 (= A23), V41 (= V39), I180 (= I177), N184 (= N181)

P08995 Nodulin-26; N-26 from Glycine max (Soybean) (Glycine hispida) (see paper)
35% identity, 96% coverage: 3:222/228 of query aligns to 38:244/271 of P08995

query
sites
P08995

K

Q

R

K

T

L

L

V

A

A

E

E

F

A

I

V

G

G

T

T

Y

Y

W

F

L

L

V

I

F

F

G

A

G

G

C

C

G

A

A

S

A

L

L

V

A

N

A

E

G

N

V

Y

P

Y

E

N

V

M

G

-

I

I

G

T

W

F

V

P

G

G

V

I

S

A

L

I

A

V

F

W

G

G

L

L

S

V

V

L

L

T

T

V

M

L

A

V

Y

Y

A

T

V

V

G

G

G

H

I

I

S

S

G

G

H

H

F

F

N

N

P

P

A

A

V

V

T

T

L

I

G

A

L

F

T

A

I

S

A

T

G

R

R

R

F

F

E

P

A

L

K

I

D

Q

I

V

P

P

P

A

Y

Y

W

V

I

V

A

A

Q

Q

L

L

G

G

G

S

A

I

A

L

A

A

A

S

G

G

T

T

L

L

F

R

V

L

I

L

M

F

S

M

G

G

Q

N

A

-

E

-

F

-

A

-

S

-

V

-

G

-

S

-

F

-

A

-

S

H

N

D

G

Q

Y

F

G

S

V

G

A

T

S

V

P

P

G

N

G

G

F

T

G

N

M

L

M

Q

S

A

G

-

M

F

I

V

A

F

E

E

V

F

V

I

L

M

T

T

A

F

V

F

F

L

V

M

I

F

V

V

I

I

L

C

G

G

-

V

A

A

T

T

S

D

H

N

M

R

V

A

P

V

E

G

G

E

F

F

A

A

P

G

I

I

A

A

I

I

G

G

L

S

C

T

L

L

T

L

L

L

I

N

H

V

L

I

I

I

S

G

I

G

P

P

I

V

T

T

N

G

T

A

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

L

A

G

M

P

A

A

I

-

Y

F

A

V

D

H

G

G

P

E

A

Y

L

E

G

G

Q

-

L

I

W

W

L

I

F

Y

W

L

A

L

A

A

P

P

L

V

A

V

G

G

A

A

V

I

I

A

G

G

A

A

L

W

I

V

W

Y

Sites not aligning to the query:

262 modified: Phosphoserine; by CPK

2evuA Crystal structure of aquaporin aqpm at 2.3a resolution (see paper)
36% identity, 99% coverage: 3:227/228 of query aligns to 6:245/245 of 2evuA

query
sites
2evuA

K

K

R

R

T

C

L

I

A

A

E

E

F

F

I

I

G

G

T

T

Y

F

W

I

L

L

V

V

F

F

G

F

G

G

C

A

G

G

A

S

A

A

L

A

L

V

A

T

A

L

G

M

V

I

P

A

E

S

V

G

G

G

I

T

G

S

-

P

-

N

-

P

-

F

-

N

-
x
I

-

G

-

I

-

G

-

L

-

G

-

L

-

G

-

D

W
|
W

V

V

G

A

V

I

S

G

L

L

A

A

F

F

G

G

L

F

S

A

V
x
I

L

A

T

A

M

S

A

I

Y

Y

A

A

V

L

G

G

G

N

I

I

S

S

G

G

G

C

H

H

F
x
I

N
|
N

P

P

A

A

V

V

T

T

L

I

G

G

L

L

T

W

I

S

A

V

G

K

R

K

F

F

E

P

A

G

K

R

D

E

I

V

P

V

P

P

Y

Y

W

I

I

I

A

A

Q

Q

L

L

G

G

G

-

A

-

A

A

A

F

G

G

T

S

L

F

F

I

V

F

I

L

M

-

S

-

G

-

Q

-

A

-

E

Q

F

C

A

A

S

G

V

I

G

G

S

A

F

A

A

T

S

V

N

G

G

G

Y

L

G
|
G

V
x
A

A

T

S
x
A

P
|
P

-
x
F

G

P

G

G

F

I

G

S

M

Y

M

W

S

Q

G

A

M

M

I

L

A

A

E

E

V

V

L

G

T

T

A

F

V

L

F

L

V

M

I

I

V

T

I

I

L

M

G

G

-

I

A

A

T

V

S

D

H

E

M

R

V

A

P

P

E

K

G

G

F

F

A

A

P

G

I

I

A

I

I

I

G

G

L

L

C

T

L

V

T

A

L

G

I

I

H

I

L

T

I

T

S

L

I

G

P

N

I

I

T

S

N

G

T

S

S

S

V

L

N

N

P

P

A

A

R

R

S

T

T

F

A

G

M

P

A

Y

I

L

Y

N

A

D

D

M

G

I

P

F

A

A

L

G

G

T

Q

D

L

L

W

W

L

N

F

Y

W

Y

A

S

-

I

-

Y

-

V

-

I

A

G

P

P

L

I

A

V

G

G

A

A

V

V

I

L

G

A

A

A

L

L

I

T

W

Y

A

Q

A

Y

L

L

D

T

E

S

binding glycerol: I44 (vs. gap), W55 (= W36), I66 (≠ V47), I81 (≠ F62), N82 (= N63), G133 (= G121), A134 (≠ V122), A136 (≠ S124), P137 (= P125), F138 (vs. gap)

8ct2D Local refinement of aqp1 tetramer (c1; refinement mask included d1 of protein 4.2 and ankyrin-1 ar1-5) in class 2 of erythrocyte ankyrin-1 complex (see paper)
36% identity, 97% coverage: 2:222/228 of query aligns to 3:225/247 of 8ct2D

query
sites
8ct2D

F

F

K

K

R

K

T

K

L

L

-

F

-

W

-

R

-

A

-

V

A

A

E

E

F

F

I

L

G

A

T

T

Y

T

W

L

L

F

V

V

F

F

G

I

G

S

C

I

G

G

A

S

A

A

L

L

-

G

-

F

-

K

L

Y

A

P

A

V

G

G

V

N

P

N

E

Q

V

T

G

A

I

V

-

Q

G

D

W

N

V

V

G

K

V

V

S

S

L

L

A

A

F

F

G

G

L

L

S

S

V

I

L

A

T

T

M

L

A

A

Y

Q

A

S

V

V

G

G

G

H

I

I

S

S

G

G

G

A

H

H

F

L

N

N

P

P

A

A

V

V

T

T

L

L

G

G

L

L

T

L

I

L

A

S

G

C

R

Q

F

I

E

S

A

I

K

F

D

R

I

A

P

L

P

M

Y

Y

W

I

I

I

A

A

Q

Q

L

C

L

V

G

G

G

A

A

I

A

V

A

A

A

T

G

A

T

-

L

-

F

-

V

-

I

I

M

L

S

S

G

G

Q

I

A

T

E

S

F

S

A

L

S

T

V

G

G

N

S

S

F

L

A

G

S

R

N

N

G

D

Y

L

-

A

-

D

G

G

V

V

A

N

S

S

P
x
G

G

Q

G

G

F

L

G
|
G

M

I

M

-

S

-

G

-

M

-

I

-

A

-

E

E

V

I

L

G

T

T

A

L

V

Q

F

L

V

V

I

L

V

C

I

V

L

L

G

A

A

T

T

T

S

D

H

R

M

R

V

R

P

R

E

D

-

L

-

G

G

G

F

S

A

A

P

P

I

L

A

A

I

I

G

G

L

L

C

S

L

V

T

A

L

L

I

G

H

H

L

L

I

L

S

A

I

I

P

D

I

Y

T

T

N

G

T

C

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

F

A

G

M

S

A

A

I

V

Y

I

A

T

D

H

G

N

P

-

A

-

L

F

G

S

Q

N

L

H

W

W

L

I

F

F

W

W

A

V

A

G

P

P

L

F

A

I

G

G

A

G

V

A

I

L

G

A

A

V

L

L

I

I

W

Y

binding cholesterol: G134 (≠ P125), G138 (= G129)

P30302 Aquaporin PIP2-3; Plasma membrane intrinsic protein 2-3; AtPIP2;3; Plasma membrane intrinsic protein 2c; PIP2c; RD28-PIP; TMP2C; Water stress-induced tonoplast intrinsic protein; WSI-TIP from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 96% coverage: 1:220/228 of query aligns to 35:262/285 of P30302

query
sites
P30302

M

L

F

Y

K

R

R

A

T

V

L

I

A

A

E

E

F

F

I

V

G

A

T

T

Y

L

W

L

L

F

V

L

F

Y

-

V

-

T

-

V

-

L

-

T

-

V

-

I

-

G

G

Y

G

K

C

I

G

Q

A

S

A

D

L

T

L

K

A

A

A

G

G

G

V

V

P

D

E

C

V

G

G

G

I

V

G

G

W

I

V

L

G

G

V

I

S

A

L

W

A

A

F

F

G

G

L

G

S

M

V

I

L

F

T

I

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

G

G

L

L

T

F

I

L

A

A

G

R

R

K

F

V

E

S

A

L

K

I

D

R

I

A

P

V

P

L

Y

Y

W

M

I

V

A

A

Q

Q

L

C

L

L

G

G

G

-

A

-

A

A

A

I

A

C

G

G

T

V

L

G

F

F

V

V

I

K

M

A

S

F

G

Q

Q

S

A

S

E

H

F

Y

A

V

S

N

V

Y

G

G

-

G

-

A

S

N

F

F

A

L

S

A

N

D

G

G

Y

Y

G

N

V

T

A

G

S

-

P

-

G

-

F

-

G

-

M

-

S

T

G

G

M

L

I

A

A

A

E

E

V

I

L

G

T

T

A

F

V

V

F

L

V

V

I

Y

V

T

I

V

L

F

G

S

A

A

T

T

-

D

-

P

-

K

-

R

-

N

-

A

-

R

-

D

S

S

H

H

M

-

V

V

P

P

E

V

G

-

F

L

A

A

P

P

I

L

A

P

I

I

G

G

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C

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T

F

L

M

I

V

H

H

L

L

I

A

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I

I

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P

I

I

T

T

N

G

T
|
T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

F

-

G

A

A

M

A

A

V

I

I

Y

F

A

N

D

K

G

S

P

K

A

P

L

W

G

D

Q

D

L

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W

W

L

I

F

F

W

W

A

V

A

G

P

P

L

F

A

I

G

G

A

A

V

T

I

I

G

A

A

A

L

F

T223 (= T182) mutation to C: Creates some mercury-sensitivity.

P55064 Aquaporin-5; AQP-5 from Homo sapiens (Human) (see 2 papers)
35% identity, 97% coverage: 2:222/228 of query aligns to 11:221/265 of P55064

query
sites
P55064

F

L

K

K

R

A

T

V

L

F

A

A

E

E

F

F

I

L

G

A

T

T

Y

L

W

I

L

F

V

V

F

F

G

F

G

G

C

L

G

G

A

S

A

A

L

L

L

K

-

W

A

P

A

S

G
x
A

V

L

P

P

E

T

V

I

G

-

I

-

G

-

W

-

V

L

G

Q

V
x
I

S

A

L

L

A

A

F

F

G

G

L

L

S

A

V

I

L

G

T

T

M

L

A

A

Y

Q

A

A

V

L

G

G

G

P

I

V

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

I

T

T

L

L

G

A

L

L

T

L

I

V

A

G

G

N

R

Q

F

I

E

S

A

L

K

L

D

R

I

A

P

F

Y

Y

W

V

I

A

A

A

Q

Q

L

L

L

V

G

G

G

A

A

I

A

A

A

G

A

A

G

G

T

I

L

L

F

Y

V

-

I

-

M

-

S

-

G

G

Q

V

A

A

E

P

F

L

A

N

S

A

V

R

G

G

S

N

F

L

A

A

S

V

N

N

G

A

Y

L

G
x
N

V

N

A

N

S

T

P

T

G

Q

G

G

F

-

G

-

M

-

S

Q

G

A

M

M

I

V

A

V

E

E

V

L

V

I

L

L

T

T

A

F

V

Q

F

L

V

A

I

L

V

C

I

I

L

F

G

A

A

S

T

T

S

D

-

S

-

R

H

R

M

T

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x
S

P

P

E

V

G

G

F

S

A

P

P

A

I

L

A

S

I

I

G

G

L

L

C

S

L

V

T

T

L

L

I

G

H

H

L

L

I

V

S

G

I
|
I

P

Y

I

F

T

T

N

G

T

C

S

S

V

M

N

N

P

P

A

A

R
|
R

S

S

T

F

A

G

M

P

A

A

I

V

Y

V

A

M

D

N

-

R

-

F

G

S

P

P

A

A

L

-

G

-

Q

-

L

H

W

W

L

V

F

F

W

W

A

V

A

G

P

P

L

I

A

V

G

G

A

A

V

V

I

L

G

A

A

A

L

I

I

L

W

Y

A38 (≠ G28) to E: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123054
I45 (≠ V39) to S: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123055
N123 (≠ G121) to D: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123057
S156 (≠ V156) mutation to A: No effect on location at the cell membrane.; mutation to E: Increased location at the cell membrane.
I177 (= I177) to F: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123056
R188 (= R188) to C: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs368292687

P43286 Aquaporin PIP2-1; Plasma membrane intrinsic protein 2-1; AtPIP2;1; Plasma membrane intrinsic protein 2a; PIP2a from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
35% identity, 96% coverage: 1:220/228 of query aligns to 37:264/287 of P43286

query
sites
P43286

M

F

F

Y

K

R

R

A

T

V

L

I

A

A

E

E

F

F

I

V

G

A

T

T

-

L

-

F

-

L

Y

Y

W

I

L

T

V

V

F

L

G

T

G

V

C

I

G

G

A

Y

A

K

L

I

L

Q

-

S

-

D

-

T

-

D

A

A

A

G

G

G

V

V

P

D

E

C

V

G

G

G

I

V

G

G

W

I

V

L

G

G

V

I

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A

L

W

A

A

F

F

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G

L

G

S

M

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I

L

F

T

I

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

G

G

L

L

T

F

I

L

A

A

G

R

R

K

F

V

E

S

A

L

K

P

D

R

I

A

P

L

Y

Y

W

I

I

I

A

A

Q

Q

L

C

L

L

G

G

G

-

A

-

A

A

A

I

A

C

G

G

T

V

L

G

F

F

V

V

I

K

M

A

S

F

G

Q

Q

S

A

S

E

Y

F

Y

A

T

S

R

V

Y

G

G

S

G

F

G

A

A

S

N

N

S

G

-

Y

-

G

-

V

-

A

-

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L

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A

G

D

G

G

F

Y

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T

M

G

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A

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A

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Y

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A

A

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-

K

-

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-

S

-

A

-

R

-

D

S

S

H

H

M

-

V

V

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P

E

V

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-

F

L

A

A

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A

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T

F

L

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H

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T

I

I

P

P

I

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

F

-

G

A

A

M

A

A

V

I

I

Y

Y

A

N

D

K

G

S

P

K

A

P

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W

G

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Q

D

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H

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W

L

I

F

F

W

W

A

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A

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P

L

F

A

I

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G

A

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A

I

I

G

A

A

A

L

F

Sites not aligning to the query:

3 modified: N6,N6-dimethyllysine; partial; K→A: 2-fold decrease in water transport activity.; K→R: No effect.
6 E→A: No effect.
280 modified: Phosphoserine; S→A: Normal subcellular localization.
283 modified: Phosphoserine; S→A: Intracellular reticulation pattern, probably corresponding to the endoplasmic reticulum.; S→D: Normal subcellular localization.

P43287 Aquaporin PIP2-2; Plasma membrane intrinsic protein 2-2; AtPIP2;2; Plasma membrane intrinsic protein 2b; PIP2b; TMP2b from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
35% identity, 96% coverage: 1:220/228 of query aligns to 35:262/285 of P43287

query
sites
P43287

M

L

F

Y

K

R

R

A

T

V

L

I

A

A

E

E

F

F

I

V

G

A

T

T

-

L

-

F

-

L

Y

Y

W

I

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V

F

L

G

T

G

V

C

I

G

G

A

Y

A

K

L

I

L

Q

-

S

-

D

-

T

-

K

A

A

A

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G

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G

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I

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G

G

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I

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A

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Y

Y

A

C

V

T

G

A

G

G

I

I

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S

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G

H

H

F

I

N

N

P

P

A

A

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V

T

T

L

F

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G

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T

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I

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A

A

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R

K

F

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S

A

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P

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Y

Y

W

M

I

V

A

A

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Q

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C

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G

G

-

A

-

A

A

A

I

A

C

G

G

T

V

L

G

F

F

V

V

I

K

M

A

S

F

G

Q

Q

S

A

S

E

Y

F

Y

A

D

S

R

V

Y

G

G

S

G

F

G

A

A

S

N

N

-

G

-

Y

-

G

-

V

-

A

S

S

L

P

A

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D

G

G

F

Y

G

N

M

T

M

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G

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M

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I

A

A

A

E

E

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A

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V

F

L

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V

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Y

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V

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S

A

A

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D

-

P

-

K

-

R

-

N

-

A

-
x
R

-
x
D

S

S

H
|
H

M

-

V

V

P

P

E

V

G

-

F

L

A

A

P

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I

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A

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I

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G

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C

A

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T

F

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H

L

L

I

A

S

T

I

I

P

P

I

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

F

-

G

A

A

M

A

A

V

I

I

Y

Y

A

N

D

K

G

S

P

K

A

P

L

W

G

D

Q

D

L

H

W

W

L

I

F

F

W

W

A

V

A

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I

G

G

A

A

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A

I

I

G

A

A

A

L

F

R194 (vs. gap) mutation to A: Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-197.
D195 (vs. gap) mutation to A: Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-197.
H197 (= H154) mutation to A: Reduced water transport activity. Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-194 or A-195.; mutation to D: Reduced water transport activity. Insensitive to cytosolic acidification.; mutation to K: Very low water transport activity. Insensitive to cytosolic acidification.

Sites not aligning to the query:

3 modified: N6,N6-dimethyllysine; partial
264 H→A: No effect.

5dyeD Crystal structure of the full length s156e mutant of human aquaporin 5 (see paper)
35% identity, 96% coverage: 3:222/228 of query aligns to 11:220/253 of 5dyeD

query
sites
5dyeD

K

K

R

A

T

V

L

F

A

A

E

E

F

F

I

L

G

A

T

T

Y

L

W

I

L

F

V

V

F

F

G

F

G

G

C

L

G

G

A

S

A

A

L

L

L

K

-

W

A

P

A

S

G

A

V

L

P

P

E

T

V

I

G

-

I

-

G

-

W

-

V

L

G

Q

V

I

S

A

L

L

A

A

F

F

G

G

L

L

S

A

V

I

L

G

T

T

M

L

A

A

Y

Q

A

A

V

L

G

G

G

P

I

V

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

I

T

T

L

L

G

A

L

L

T

L

I

V

A

G

G

N

R

Q

F

I

E

S

A

L

K

L

D

R

I

A

P

F

Y

Y

W

V

I

A

A

A

Q

Q

L

L

L

V

G

G

G

A

A

I

A

A

A

G

A

A

G

G

T

I

L

L

F

Y

V

-

I

-

M

-

S

-

G

G

Q

V

A

A

E

P

F

L

A

N

S

A

V

R

G

G

S

N

F

L

A

A

S

V

N

N

G

A

Y

L

G

N

V

N

A

N

S

T

P

T

G

Q

G

G

F

-

G

-

M

-

S

Q

G

A

M

M

I

V

A

V

E

E

V

L

V

I

L

L

T

T

A

F

V

Q

F

L

V

A

I

L

V

C

I

I

L

F

G

A

A

S

T

T

S

D

-

S

-

R

H

R

M

T

V

E

P

P

E

V

G
|
G

F
x
S

A

P

P

A

I

L

A

S

I

I

G

G

L

L

C

S

L

V

T

T

L

L

I

G

H

H

L

L

I

V

S

G

I

I

P

Y

I

F

T

T

N

G

T

C

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

F

A

G

M

P

A

A

I

V

Y

V

A

M

D

N

G

R

P

F

A

S

L

P

G

A

Q

H

L

-

W

W

L

V

F

F

W

W

A

V

A

G

P

P

L

I

A

V

G

G

A

A

V

V

I

L

G

A

A

A

L

I

I

L

W

Y

binding O-[(S)-{[(2S)-2-(hexanoyloxy)-3-(tetradecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-D-serine: G158 (= G159), S159 (≠ F160)

Q08733 Aquaporin PIP1-3; AtPIP1;3; Plasma membrane intrinsic protein 1c; PIP1c; Transmembrane protein B; TMP-B from Arabidopsis thaliana (Mouse-ear cress) (see paper)
34% identity, 96% coverage: 1:220/228 of query aligns to 50:271/286 of Q08733

query
sites
Q08733

M

F

F

Y

K

R

R

A

T

G

L

I

A

A

E

E

F

F

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I

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A

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A

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A

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A

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P

E

N

V

M

-

C

-

A

G

S

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Q

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G

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I

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A

L

W

A

A

F

F

G

G

L

G

S

M

V

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F

T

A

M

L

A

V

Y

Y

A

C

V

T

G

A

G

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I

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S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

G

G

L

L

T

F

I

L

A

A

G

R

R

K

F

L

E

S

A

L

K

T

D

R

I

A

P

V

P

F

Y

Y

W

I

I

V

A

M

Q

Q

L

C

L

L

G

G

G

A

A

I

A

C

A

G

A

A

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G

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V

L

-

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-

V

-

I

-

M

-

S

-

G

-

Q

-

A

-

E

-

F

-

A

-

S

-

V

V

G

K

S

G

F

F

A

Q

S

P

N

N

G

P

Y

Y

G

Q

V

T

A

L

S

G

P

G

G

G

-

A

-

N

-

T

-

V

-

A

-

H

G

G

F

Y

G

T

M

K

M

G

S

S

G

G

M

L

I

G

A

A

E

E

V

I

L

G

T

T

A

F

V

V

F

L

V

V

I

Y

V

T

I

V

L

F

G

S

A

A

T

T

-

D

-

A

-

K

-

R

-

S

-

A

-

R

-

D

S

S

H

H

M

-

V

V

P

P

E

I

G

-

F

L

A

A

P

P

I

L

A

P

I

I

G

G

L

F

C

A

L

V

T

F

L

L

I

V

H

H

L

L

I

A

S

T

I

I

P

P

I

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

L

A

G

M

A

A

A

-

I

I

I

Y

Y

A

N

D

K

G

D

P

H

A

A

L

W

G

D

Q

D

L

H

W

W

L

I

F

F

W

W

A

V

A

G

P

P

L

F

A

I

G

G

A

A

V

A

I

L

G

A

A

A

L

L

Sites not aligning to the query:

1 modified: N-acetylmethionine

Q9XF58 Aquaporin PIP2-5; Plasma membrane intrinsic protein 2-5; ZmPIP2-5; ZmPIP2;5; ZmPIP2a from Zea mays (Maize) (see paper)
33% identity, 96% coverage: 1:219/228 of query aligns to 35:263/285 of Q9XF58

query
sites
Q9XF58

M

L

F

Y

K

R

R

A

T

V

L

I

A

A

E

E

F

F

I

V

G

A

T

T

Y

L

W

L

L

F

V

L

F

Y

-

I

-

T

-

V

-

A

-

T

-

V

-

I

G

G

G

Y

C

K

G

H

A

Q

A

T

L

D

L

A

A

A

G

S

V

G

P

P

E

D

V

A

-

A

-

C

-

G

G

G

I

V

G

G

W

V

V

L

G

G

V

I

S

A

L

W

A

A

F

F

G

G

L

G

S

M

V

I

L

F

T

I

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

V

S

S

G

G

G
|
G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

G

G

L

L

T

F

I

L

A

A

G

R

R

K

F

V

E

S

A

L

K

V

D

R

I

A

P

L

Y

Y

W

I

I

V

A

A

Q

Q

L

C

L

L

G

G

G

A

A

I

A

C

A

G

A

V

G

G

T

-

L

-

F

-

V

L

I

V

M

K

S

G

G

F

Q

Q

A

S

E

A

F

F

A

-

S

-

V

-

G

-

S

-

F

Y

A

V

S

R

N

Y

G

G

Y

G

G

G

V

A

A

N

S

E

-

L

P

S

G

A

G

G

F

Y

G

S

M

K

M

G

S

T

G

G

M

L

I

A

A

A

E

E

V

I

L

G

T

T

A

F

V

V

F

L

V

V

I

Y

V

T

I

V

L

F

G

S

A

A

T

T

-

D

-

P

-

K

-

R

-

N

-

A

-

R

-

D

S

S

H

H

M

-

V

V

P

P

E

V

G

-

F

L

A

A

P

P

I

L

A

P

I

I

G

G

L

F

C

A

L

V

T

F

L

M

I

V

H

H

L

L

I

A

S

T

I

I

P

P

I

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

L

-

G

A

A

M

A

A

V

I

I

Y

Y

A

N

D

N

G

D

P

K

A

A

L

W

G

D

Q

D

L

H

W

W

L

I

F

F

W

W

A

V

A

G

P

P

L

F

A

I

G

G

A

A

V

A

I

I

G

A

A

A

G104 (= G60) mutation to W: Loss of water transport.

Q39196 Probable aquaporin PIP1-4; Plasma membrane intrinsic protein 1-4; AtPIP1;4; Transmembrane protein C; TMP-C from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 96% coverage: 1:220/228 of query aligns to 51:272/287 of Q39196

query
sites
Q39196

M

F

F

Y

K

R

R

A

T

G

L

I

A

A

E

E

F

F

I

I

G

A

T

T

Y

F

W

L

L

F

V

L

F

Y

G

I

G

T

C

V

G

L

A

T

A

V

L

M

L

G

A

V

A

K

G

R

V

A

P

P

E

N

V

M

-

C

-

A

G

S

I

V

G

G

W

I

V

Q

G

G

V

I

S

A

L

W

A

A

F

F

G

G

L

G

S

M

V

I

L

F

T

A

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

G

G

L

L

T

F

I

L

A

A

G

R

R

K

F

L

E

S

A

L

K

T

D

R

I

A

P

V

P

F

Y

Y

W

M

I

I

A

M

Q

Q

L

C

L

L

G

G

G

A

A

I

A

C

A

G

A

A

G

G

T

-

L

-

F

-

V

V

I

V

M

K

S

G

G

F

Q

Q

-

P

A

T

E

P

F

Y

A

Q

S

T

V

L

G

G

S

G

F

G

A

A

S

N

N

T

G

-

Y

-

G

-

V

V

A

A

S

H

P

-

G

-

G

G

F

Y

G

T

M

K

M

G

S

S

G

G

M

L

I

G

A

A

E

E

V

I

L

G

T

T

A

F

V

V

F

L

V

V

I

Y

V

T

I

V

L

F

G

S

A

A

T

T

-

D

-

A

-

K

-

R

-

S

-

A

-

R

-

D

S

S

H

H

M

-

V

V

P

P

E

I

G

-

F

L

A

A

P

P

I

L

A

P

I

I

G

G

L

F

C

A

L

V

T

F

L

L

I

V

H

H

L

L

I

A

S

T

I

I

P

P

I

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

L

A

G

M

A

A

A

-

I

I

I

Y

Y

A

N

D

K

G

D

P

H

A

S

L

W

G

D

Q

D

L

H

W

W

L

I

F

F

W

W

A

V

A

G

P

P

L

F

A

I

G

G

A

A

V

A

I

L

G

A

A

A

L

L

Sites not aligning to the query:

1 modified: N-acetylmethionine

P61837 Aquaporin PIP1-1; AtPIP1;1; Plasma membrane aquaporin-1; Plasma membrane intrinsic protein 1a; PIP1a from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 94% coverage: 6:220/228 of query aligns to 55:271/286 of P61837

query
sites
P61837

L

I

A

A

E

E

F

F

I

I

G

A

T

T

Y

F

W

L

L

F

V

L

F

Y

G

I

G

T

C

V

G

L

A

T

A

V

L

M

L

G

A

V

A

K

G

R

V

S

P

P

E

N

V

M

-

C

-

A

G

S

I

V

G

G

W

I

V

Q

G

G

V

I

S

A

L

W

A

A

F

F

G

G

L

G

S

M

V

I

L

F

T

A

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

G

G

L

L

T

F

I

L

A

A

G

R

R

K

F

L

E

S

A

L

K

T

D

R

I

A

P

L

P

Y

Y

Y

W

I

I

V

A

M

Q

Q

L

C

L

L

G

G

G

A

A

I

A

C

A

G

A

A

G

G

T

V

L

V

F

K

V

G

I

F

M

Q

S

P

G

K

Q

Q

A

-

E

-

F

Y

A

Q

S

A

V

L

G

G

S

G

F

G

A

A

S

N

N

T

G

-

Y

-

G

-

V

V

A

A

S

H

P

-

G

-

G

G

F

Y

G

T

M

K

M

G

S

S

G

G

M

L

I

G

A

A

E

E

V

I

L

G

T

T

A

F

V

V

F

L

V

V

I

Y

V

T

I

V

L

F

G

S

A

A

T

T

-

D

-

A

-

K

-

R

-

N

-

A

-

R

-

D

S

S

H

H

M

-

V

V

P

P

E

I

G

-

F

L

A

A

P

P

I

L

A

P

I

I

G

G

L

F

C

A

L

V

T

F

L

L

I

V

H

H

L

L

I

A

S

T

I

I

P

P

I

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

L

A

G

M

A

A

A

-

I

I

I

Y

Y

A

N

D

K

G

D

P

H

A

S

L

W

G

D

Q

D

L

H

W

W

L

V

F

F

W

W

A

V

A

G

P

P

L

F

A

I

G

G

A

A

V

A

I

L

G

A

A

A

L

L

Sites not aligning to the query:

1 modified: N-acetylmethionine

P56402 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Mus musculus (Mouse) (see 2 papers)
34% identity, 98% coverage: 3:225/228 of query aligns to 11:222/271 of P56402

query
sites
P56402

K

R

R

A

T

V

L

L

A

A

E

E

F

F

I

L

G

A

T

T

Y

L

W

L

L

F

V

V

F

F

G

F

G

G

C

L

G

G

A

S

A

A

L

L

L

Q

A

W

A

A

G

S

V

S

P

P

E

P

V

-

G

-

I

-

G

S

W

V

V

L

G

Q

V

I

S

A

L

V

A

A

F

F

G

G

L

L

S

G

V

I

L

G

T

T

M

L

A

V

Y

Q

A

A

V

L

G

G

G

H

I

V

S

S

G

G

G

A

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

V

G

A

L

C

T

L

I

V

A

G

G

C

R

H

F

V

E

S

A

F

K

L

D

R

I

A

P

A

P

F

Y

Y

W

V

I

A

A

A

Q

Q

L

L

G

G

G

A

A

V

A

A

A

G

A

A

G

A

T

I

L

L

F

H

V

E

I

I

M

T

S

P

G

V

Q

E

A

I

E

R

F

-

A

-

S

-

V

-

G

G

S

D

F

L

A

A

S

V

N

N

G

A

Y

L

G

H

V

N

A

N

S

A

P
x
T

G

A

G

G

F

-

G

-

M

-

S

Q

G

A

M

V

I

T

A

V

E

E

V

L

V

F

L

L

T

T

A

M

V

Q

F

L

V

V

I

L

V

C

I

I

L

F

G

A

A

S

T

T

S

D

H

E

M

R

V

R

P

S

E

D

-

N

-

L

G

G

F

S

A

P

P

A

I

L

A

S

I

I

G

G

L

F

C

S

L

V

T

T

L

L

I

G

H

H

L

L

I

L

S

G

I

I

P

Y

I

F

T

T

N

G

T

C

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

L

A

A

M

P

A

A

I

V

Y

V

A

T

D

G

G

-

P

-

A

K

L

F

G

D

Q

D

L

H

W

W

L

V

F

F

W

W

A

I

A

G

P

P

L

L

A

V

G

G

A

A

V

V

I

I

G

G

A

S

L

L

I

L

W

Y

A

N

A

Y

L

L

T126 (≠ P125) mutation to M: Does not cause loss of water channel activity, but impairs trafficking from cytoplasmic vesicles to the cell membrane.

Sites not aligning to the query:

256 modified: Phosphoserine; S → L: in cph; loss of a phosphorylation site and loss of trafficking to the apical cell membrane; causes aberrant location at the basolateral cell membrane

Q06611 Aquaporin PIP1-2; AtPIP1;2; Plasma membrane intrinsic protein 1b; PIP1b; Transmembrane protein A; AthH2; TMP-A from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 94% coverage: 6:220/228 of query aligns to 55:271/286 of Q06611

query
sites
Q06611

L

I

A

A

E

E

F

F

I

I

G

A

T

T

Y

F

W

L

L

F

V

L

F

Y

G

I

G

T

C

V

G

L

A

T

A

V

L

M

L

G

A

V

A

K

G

R

V

S

P

P

E

N

V

M

-

C

-

A

G

S

I

V

G

G

W

I

V

Q

G

G

V

I

S

A

L

W

A

A

F

F

G

G

L

G

S

M

V

I

L

F

T

A

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

G

G

L

L

T

F

I

L

A

A

G

R

R

K

F

L

E

S

A

L

K

T

D

R

I

A

P

V

P

Y

Y

Y

W

I

I

V

A

M

Q

Q

L

C

L

L

G

G

G

A

A

I

A

C

A

G

A

A

G

G

T

V

L

V

F

K

V

G

I

F

M

Q

S

P

G

K

Q

Q

A

-

E

-

F

Y

A

Q

S

A

V

L

G

G

S

G

F

G

A

A

S

N

N

T

G

-

Y

-

G

-

V

I

A

A

S

H

P

-

G

-

G

G

F

Y

G

T

M

K

M

G

S

S

G

G

M

L

I

G

A

A

E

E

V

I

L

G

T

T

A

F

V

V

F

L

V

V

I

Y

V

T

I

V

L

F

G

S

A

A

T

T

-

D

-

A

-

K

-

R

-

N

-

A

-

R

-

D

S

S

H

H

M

-

V

V

P

P

E

I

G

-

F

L

A

A

P

P

I

L

A

P

I

I

G

G

L

F

C

A

L

V

T

F

L

L

I

V

H

H

L

L

I

A

S

T

I

I

P

P

I

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

L

A

G

M

A

A

A

-

I

I

I

Y

F

A

N

D

K

G

D

P

N

A

A

L

W

G

D

Q

D

L

H

W

W

L

V

F

F

W

W

A

V

A

G

P

P

L

F

A

I

G

G

A

A

V

A

I

L

G

A

A

A

L

L

Sites not aligning to the query:

1 modified: N-acetylmethionine

4nefA X-ray structure of human aquaporin 2 (see paper)
33% identity, 96% coverage: 3:222/228 of query aligns to 10:218/239 of 4nefA

query
sites
4nefA

K

R

R

A

T

V

L

F

A

A

E

E

F

F

I

L

G

A

T

T

Y

L

W

L

L

F

V

V

F

F

G

F

G

G

C

L

G

G

A

S

A

A

L

L

L

-

A

-

G

N

V

W

P

P

E

Q

V

A

G

L

I

P

G

S

W

V

V

L

G

Q

V

I

S

A

L

M

A

A

F

F

G

G

L

L

S

G

V

I

L

G

T

T

M

L

A

V

Y

Q

A

A

V

L

G

G

G

H

I

I

S

S

G

G

G

A

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

V

G

A

L
x
C

T

L

I

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A

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G

C

R
x
H

F

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A

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L

D

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A

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Y

W

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A

A

A

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Q

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L

G

G

G

A

A

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A

A

A

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A

A

G

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L

L

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H

V

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I

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P

G

A

Q

D

A

I

E

R

F

-

A

-

S

-

V

-

G

G

S

D

F

L

A

A

S

V

N

N

G

A

Y

L

G

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A

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S

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T

G

A

G

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-

G

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G

A

A

S

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S

D

H

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M

R

V

R

P

G

E

E

-

N

-

P

G

G

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A

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A

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I

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L

L

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H

L

L

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L

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G

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Y

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N

N

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P

A

A

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S

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A

A

M

P

A

A

I

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Y

V

A

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D

G

G

-

P

-

A

K

L

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G

D

Q

D

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W

W

L

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F

F

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W

A

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A

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P

L

L

A

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G

G

A

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L

G

G

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L

L

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L

W

Y

binding zinc ion: C74 (≠ L70), H79 (≠ R75)

P41181 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Homo sapiens (Human) (see 12 papers)
33% identity, 96% coverage: 3:222/228 of query aligns to 11:219/271 of P41181

query
sites
P41181

K

R

R

A

T

V

L

F

A

A

E

E

F

F

I

L

G

A

T

T

Y

L

W

L

L

F

V

V

F

F

G

F

G

G

C

L

G

G

A

S

A

A

L

L

L

-

A

-

G

N

V

W

P

P

E

Q

V

A

G

L

I

P

G

S

W

V

V

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G

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A

L

M

A

A

F

F

G

G

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L

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G

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T

M

L

A

V

Y

Q

A

A

V

L

G

G

G

H

I

I

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S

G
|
G

G

A

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

V

G

A

L

C

T

L

I

V

A
x
G

G
x
C

R

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F

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S

A

V

K

L

D

R

I

A

P

A

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Y

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A

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Q

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L

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G

G

A

A

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A

A

A

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A

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G

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L

L

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I

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G

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A

I

E

R

F

-

A

-

S

-

V

-

G

G

S

D

F

L

A

A

S

V

N

N

G

A

Y

L

G

S

V

N

A

S

S

T

P

T

G

A

G

G

F

-

G

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M

-

S

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M

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A

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E

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V

F

L

L

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T

A

L

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F

L

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C

I

I

L

F

G

A

A
x
S

T

T

S

D

H

E

M

R

V

R

P

G

E

E

-

N

-

P

G

G

F

T

A

P

P

A

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A

S

I

I

G

G

L

F

C

S

L

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A

L

L

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H

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L

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G

I

I

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H

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G

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R
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R

S

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A
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A

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A

A

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Y
x
V

A

T

D

G

G

-

P

-

A

K

L

F

G

D

Q

D

L

H

W

W

L

V

F

F

W

W

A

I

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P

L

L

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V

G

G

A

A

V

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I

L

G

G

A
x
S

L
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L

I

L

W

Y

G64 (= G59) to R: in NDI2; loss of water channel activity; dbSNP:rs104894326
G78 (≠ A73) mutation to A: Does not affect interaction with MIAC; when associated with A-79.
C79 (≠ G74) mutation to A: Does not affect interaction with MIAC; when associated with A-78.
S148 (≠ A151) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to D: Retained in the endoplasmic reticulum.
R187 (= R188) to C: in NDI2; loss of water channel activity; mutant protein does not fold properly; dbSNP:rs104894328
A190 (= A191) to T: in NDI2; mutant protein does not fold properly and is not functional; dbSNP:rs104894341
V194 (≠ Y195) to I: in dbSNP:rs772051028
S216 (≠ A219) to P: in NDI2; loss of water channel activity; dbSNP:rs104894329
L217 (= L220) mutation to A: Abolishes interaction with MIAC; when associated with A-221.

Sites not aligning to the query:

221 Y→A: Abolishes interaction with MIAC; when associated with A-217.
229 S→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; S→D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
231 S→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; S→D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
232 E→A: Reduces interaction with MIAC.
244 T→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; T→E: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
254 R → L: in NDI2; results in the loss of arginine vasopressin-mediated phosphorylation at S-256; R → Q: in NDI2; exerts a dominant-negative effect on wild-type-AQP2 in that it interferes with its trafficking to the apical membrane; is a loss of function instead of a gain of function mutation on dominant nephrogenic diabetes insipidus
256 modified: Phosphoserine; by PKA; S→A: Retained in vesicles.; S→D: Expressed in the apical membrane.
258 E → K: in NDI2; retained in the Golgi compartment; dbSNP:rs104894332
262 P → L: in NDI2; mutant protein folds properly and is functional but is retained in intracellular vesicles; able to assemble into tetramers with wild-type AQP2 that properly localize to the apical membrane; dbSNP:rs104894339; P→A: No effect on expression at the apical cell membrane.

P06624 Lens fiber major intrinsic protein; Aquaporin-0; MIP26; MP26 from Bos taurus (Bovine) (see 4 papers)
35% identity, 95% coverage: 7:222/228 of query aligns to 15:219/263 of P06624

query
sites
P06624

A

A

E

E

F

F

I

F

G

A

T

S

Y

L

W

F

L

Y

V

V

F

F

G

F

G

G

C

L

G

G

A

A

A

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L

L

L

R

A

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A

A

G

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G

P

P

E

-

V

-

G

-

I

L

G

H

W

V

V

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V

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A

L

L

A

A

F

F

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L

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A

A

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V

G

G

G

H

I

I

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S

G

G

G

A

H

H

F

V

N

N

P

P

A

A

V

V

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T

L

F

G

A

L

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Y

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A

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Q

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L

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G

G

A

A

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A

A

A

G

A

A

G

A

T

V

L

L

F

Y

V

S

I

V

M

T

S

P

G

P

Q

-

A

-

E

-

F

-

A

A

S

V

V

R

G

G

S

N

F

L

A

A

S

L

N

N

G

-

Y

-

G

-

V

T

A

L

S

H

P

P

G

-

G

G

F

V

G

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M

V

M

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G

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M

T

I

I

A

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E

E

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L

T

T

A

L

V

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F

F

V

V

I

L

V

C

I

I

L

F

G

A

A

T

T
x
Y

S

D

H

E

M

R

V

R

-

N

-

G

P

R

E

L

G

G

F

S

A

V

P

A

I

L

A

A

I

V

G

G

L

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C

S

L

L

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T

L

L

I

G

H

H

L

L

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F

S

G

I

M

P

Y

I

Y

T

T

N

G

T

A

S

G

V

M

N

N

P

P

A

A

R

R

S

S

T

F

A

A

M

P

A

A

I

I

Y

L

A

T

D

R

G

N

P

-

A

-

L

F

G

T

Q

N

L

H

W

W

L

V

F

Y

W

W

A

V

A

G

P

P

L

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A

I

G

G

A

A

V

G

I

L

G

G

A

S

L

L

I

L

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Y

Y149 (≠ T152) mutation to G: Increases constitutive water permeability. Abolishes regulation by cytoplasmic calcium levels.; mutation to L: Strongly decreases water permeability. Abolishes regulation by cytoplasmic calcium levels.; mutation to S: Slightly decreases water permeability, but has a minor effect on the regulation by cytoplasmic calcium levels.

Sites not aligning to the query:

227 L→A: Strongly reduced CALM binding.
227:237 Interaction with CALM
230 V→A: Strongly reduced CALM binding.
235 modified: Phosphoserine
243 modified: Phosphoserine; by PKA
245 modified: Phosphoserine
246 Interaction with BFSP1; modified: Deamidated asparagine
250 interaction with BFSP1

Query Sequence

>3607331 FitnessBrowser__Dino:3607331
MFKRTLAEFIGTYWLVFGGCGAALLAAGVPEVGIGWVGVSLAFGLSVLTMAYAVGGISGG
HFNPAVTLGLTIAGRFEAKDIPPYWIAQLLGGAAAAGTLFVIMSGQAEFASVGSFASNGY
GVASPGGFGMMSGMIAEVVLTAVFVIVILGATSHMVPEGFAPIAIGLCLTLIHLISIPIT
NTSVNPARSTAMAIYADGPALGQLWLFWAAPLAGAVIGALIWAALDED

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory