SitesBLAST
Comparing 3607390 FitnessBrowser__Dino:3607390 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
37% identity, 98% coverage: 2:532/540 of query aligns to 4:455/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G9), G13 (= G11), S14 (= S12), A15 (= A13), E35 (= E33), A36 (= A34), W47 (= W60), P65 (= P78), G67 (= G80), V180 (= V217), A214 (≠ G252), G215 (= G253), A218 (≠ N256), T270 (≠ M334), Y391 (= Y468), A424 (≠ C501), I435 (≠ T512), N436 (= N513)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
35% identity, 97% coverage: 4:527/540 of query aligns to 25:569/578 of 5nccA
- active site: R347 (= R319), L420 (≠ V380), I421 (≠ C381), S507 (≠ I467), A509 (≠ H469), G552 (= G510), Q553 (≠ N511)
- binding flavin-adenine dinucleotide: G30 (= G9), G32 (= G11), T33 (≠ S12), A34 (= A13), L53 (= L32), E54 (= E33), A55 (= A34), F74 (≠ M53), W80 (= W60), A98 (≠ P78), G100 (= G80), G105 (= G85), S106 (= S86), N110 (= N90), A111 (≠ G91), T112 (≠ L92), L113 (= L93), V238 (= V217), A278 (≠ G252), H282 (≠ N256), L286 (≠ I260), N508 (≠ Y468), Q553 (≠ N511), T554 (= T512), G555 (≠ N513), V558 (≠ A516)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
34% identity, 98% coverage: 2:530/540 of query aligns to 1:564/565 of 5oc1A
- active site: V339 (≠ I320), N413 (≠ V380), A414 (≠ C381), I499 (= I467), H501 (= H469), A544 (≠ G510), H545 (≠ N511)
- binding 4-methoxybenzoic acid: Y91 (≠ G91), I356 (≠ M334), I390 (≠ D364), F396 (≠ V370), T412 (≠ S379), I499 (= I467), H501 (= H469), H545 (≠ N511)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), N11 (≠ S12), A12 (= A13), E32 (= E33), A33 (= A34), W60 (= W60), P78 (= P78), G80 (= G80), G85 (= G85), S86 (= S86), H90 (≠ N90), Y91 (≠ G91), V93 (≠ L93), V230 (= V217), S270 (= S251), A271 (≠ G252), G272 (= G253), F500 (≠ Y468), H545 (≠ N511), T546 (= T512), Q547 (≠ N513), I550 (≠ A516)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
34% identity, 98% coverage: 2:530/540 of query aligns to 1:564/565 of 3fimB
- active site: V339 (≠ I320), N413 (≠ V380), A414 (≠ C381), I499 (= I467), H501 (= H469), A544 (≠ G510), H545 (≠ N511)
- binding flavin-adenine dinucleotide: G8 (= G9), N11 (≠ S12), A12 (= A13), E32 (= E33), A33 (= A34), W60 (= W60), P78 (= P78), G80 (= G80), G85 (= G85), S86 (= S86), H90 (≠ N90), Y91 (≠ G91), V93 (≠ L93), V230 (= V217), S270 (= S251), A271 (≠ G252), F500 (≠ Y468), H501 (= H469), H545 (≠ N511), T546 (= T512), Q547 (≠ N513), I550 (≠ A516)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
35% identity, 97% coverage: 4:527/540 of query aligns to 85:636/654 of A0A248QE08
- TA 93:94 (≠ SA 12:13) binding
- E114 (= E33) binding
- L162 (≠ V82) binding
- S166 (= S86) binding
- NATL 170:173 (≠ NGLL 90:93) binding
- V298 (= V217) binding
- C432 (≠ S337) binding
- R451 (≠ H357) binding
- Y466 (≠ F373) binding
- Q486 (≠ S379) binding
- G622 (≠ N513) binding
6yrvAAA structure of fap after illumination at 100k (see paper)
35% identity, 99% coverage: 4:537/540 of query aligns to 9:570/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H357), N499 (≠ Y468)
- binding flavin-adenine dinucleotide: G14 (= G9), G16 (= G11), T17 (≠ S12), A18 (= A13), L37 (= L32), E38 (= E33), A39 (= A34), F58 (≠ M53), W64 (= W60), A82 (≠ P78), G89 (= G85), S90 (= S86), N94 (= N90), A95 (≠ G91), T96 (≠ L92), L97 (= L93), M191 (≠ T186), V222 (= V217), C264 (≠ S251), A265 (≠ G252), G266 (= G253), H269 (≠ N256), N499 (≠ Y468), A534 (≠ C501), Q544 (≠ N511), T545 (= T512), G546 (≠ N513)
- binding heptadecane: V377 (≠ Q359), G379 (≠ W361), M380 (≠ S362), G386 (= G369), T389 (≠ P372), Y390 (≠ F373), F393 (vs. gap), T408 (= T377), Q410 (≠ S379)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
34% identity, 96% coverage: 4:524/540 of query aligns to 3:501/508 of 4ha6A
- active site: F360 (≠ V380), G361 (≠ C381), H444 (≠ I467), H446 (= H469), G487 (= G510), P488 (≠ N511)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), S11 (= S12), A12 (= A13), E32 (= E33), A33 (= A34), W58 (= W60), R77 (= R79), G78 (= G80), G83 (= G85), S84 (= S86), L87 (= L89), H88 (≠ N90), A89 (≠ G91), M90 (≠ L92), G91 (≠ L93), V218 (= V217), A251 (≠ G252), G252 (= G253), E255 (≠ N256), H445 (≠ Y468), A478 (≠ C501), P488 (≠ N511), I489 (≠ T512), H490 (≠ N513)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (≠ G91), S314 (≠ A321), H444 (≠ I467), H446 (= H469)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
34% identity, 96% coverage: 4:524/540 of query aligns to 3:501/509 of 3t37A
- active site: F360 (≠ V380), G361 (≠ C381), H444 (≠ I467), H446 (= H469), G487 (= G510), P488 (≠ N511)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), S11 (= S12), A12 (= A13), E32 (= E33), A33 (= A34), W58 (= W60), R77 (= R79), G78 (= G80), R79 (≠ K81), G83 (= G85), S84 (= S86), H88 (≠ N90), A89 (≠ G91), G91 (≠ L93), R217 (≠ L216), V218 (= V217), A251 (≠ G252), E255 (≠ N256), H445 (≠ Y468), A478 (≠ C501), P488 (≠ N511), I489 (≠ T512), H490 (≠ N513)
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
32% identity, 98% coverage: 1:527/540 of query aligns to 14:572/577 of 4h7uA
- active site: A343 (≠ S313), V426 (≠ C381), Y510 (≠ I467), H512 (= H469), A555 (≠ G510), H556 (≠ N511)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G9), G24 (= G11), T25 (≠ S12), A26 (= A13), E46 (= E33), A47 (= A34), W74 (= W60), G99 (= G85), C100 (≠ S86), H103 (≠ L89), N104 (= N90), G105 (= G91), V107 (≠ L93), L242 (= L216), V243 (= V217), G282 (= G252), G283 (= G253), A286 (≠ N256), H512 (= H469), A546 (≠ C501), H556 (≠ N511), T557 (= T512), Q558 (≠ N513), V561 (≠ A516)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
32% identity, 98% coverage: 1:527/540 of query aligns to 39:597/602 of Q3L245
- N100 (≠ C61) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ L89) modified: Tele-8alpha-FAD histidine
- N344 (= N289) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H469) active site, Proton acceptor
- H581 (≠ N511) active site
Sites not aligning to the query:
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
34% identity, 98% coverage: 4:531/540 of query aligns to 7:531/531 of E4QP00
- V101 (≠ L89) mutation to H: Abolishes activity.
- M103 (≠ G91) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (= V380) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ Q382) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ I467) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ Y468) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H469) mutation to A: Abolishes activity.
- N511 (= N511) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
34% identity, 97% coverage: 4:528/540 of query aligns to 3:524/525 of 4udqA
- active site: L331 (= L326), F364 (≠ C381), W365 (≠ Q382), V461 (≠ I467), H463 (= H469), A506 (≠ G510), N507 (= N511)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), T11 (≠ S12), A12 (= A13), E32 (= E33), A33 (= A34), W64 (≠ M53), G88 (= G80), G93 (= G85), G94 (≠ S86), N98 (= N90), M99 (≠ G91), V101 (≠ L93), V229 (= V217), T261 (≠ S251), A262 (≠ G252), W462 (≠ Y468), H463 (= H469), A497 (≠ C501), N507 (= N511), T508 (= T512), N509 (= N513), T512 (≠ A516)
4ynuA Crystal structure of aspergillus flavus fadgdh in complex with d- glucono-1,5-lactone (see paper)
32% identity, 98% coverage: 4:532/540 of query aligns to 5:567/569 of 4ynuA
- active site: V341 (≠ E310), F412 (≠ V380), W413 (≠ C381), N501 (≠ I467), H503 (= H469), G545 (= G510), H546 (≠ N511)
- binding flavin-adenine dinucleotide: G12 (= G11), T13 (≠ S12), S14 (≠ A13), E34 (= E33), A35 (= A34), Y51 (= Y49), F55 (≠ H54), W61 (= W60), R79 (≠ P78), G81 (= G80), G86 (= G85), T87 (≠ S86), N91 (= N90), G92 (= G91), T232 (≠ L216), A233 (≠ V217), A273 (≠ G252), G274 (= G253), R277 (≠ N256), F502 (≠ Y468), A536 (≠ C501), H546 (≠ N511), L547 (≠ T512), V548 (≠ N513), L551 (≠ A516)
- binding D-glucono-1,5-lactone: Y51 (= Y49), E411 (≠ S379), A496 (≠ S462), N497 (= N463), R499 (≠ V465), R499 (≠ V465), N501 (≠ I467), H503 (= H469), H546 (≠ N511)
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
32% identity, 98% coverage: 4:532/540 of query aligns to 6:568/570 of 4yntA
- active site: V342 (≠ E310), F413 (≠ V380), W414 (≠ C381), N502 (≠ I467), H504 (= H469), G546 (= G510), H547 (≠ N511)
- binding dihydroflavine-adenine dinucleotide: G13 (= G11), T14 (≠ S12), S15 (≠ A13), E35 (= E33), A36 (= A34), F56 (≠ H54), W62 (= W60), R80 (≠ P78), G82 (= G80), G87 (= G85), T88 (≠ S86), N92 (= N90), G93 (= G91), M94 (≠ L92), A95 (≠ L93), A234 (≠ V217), A274 (≠ G252), R278 (≠ N256), F503 (≠ Y468), A537 (≠ C501), H547 (≠ N511), L548 (≠ T512), V549 (≠ N513), L552 (≠ A516)
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
32% identity, 98% coverage: 4:531/540 of query aligns to 5:566/566 of 7vzsA
- binding D-glucal: Y6 (= Y5), L22 (= L21), N25 (≠ D24), Y51 (= Y49), I349 (≠ A318), Q356 (≠ A325), E411 (≠ S379), E444 (= E412), W445 (≠ T413), K448 (≠ A416), R499 (≠ V465), N501 (≠ I467), H546 (≠ N511), K563 (≠ L528), A566 (= A531)
- binding flavin-adenine dinucleotide: G10 (= G9), G12 (= G11), T13 (≠ S12), S14 (≠ A13), E34 (= E33), A35 (= A34), Y51 (= Y49), F55 (≠ H54), W61 (= W60), R79 (≠ P78), G81 (= G80), G86 (= G85), T87 (≠ S86), N91 (= N90), G92 (= G91), M93 (≠ L92), A94 (≠ L93), T232 (≠ L216), A233 (≠ V217), A273 (≠ G252), G274 (= G253), R277 (≠ N256), F502 (≠ Y468), A536 (≠ C501), H546 (≠ N511), L547 (≠ T512), V548 (≠ N513), L551 (≠ A516)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
33% identity, 97% coverage: 2:527/540 of query aligns to 13:526/527 of 2jbvA
- active site: I333 (≠ A329), P377 (≠ V380), N378 (≠ C381), V464 (≠ I467), H466 (= H469), V509 (≠ G510), N510 (= N511)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G11), S23 (= S12), E44 (= E33), A45 (= A34), W71 (= W60), A90 (≠ G80), G95 (= G85), C96 (≠ S86), H99 (≠ L89), N100 (= N90), S101 (≠ G91), I103 (≠ L93), R231 (≠ L216), A232 (≠ V217), T269 (≠ G252), G270 (= G253), D273 (≠ N256), V464 (≠ I467), Y465 (= Y468), H466 (= H469), D499 (= D500), A500 (≠ C501), N510 (= N511), P511 (≠ T512), N512 (= N513), V515 (≠ A516)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
33% identity, 97% coverage: 2:527/540 of query aligns to 13:526/532 of 4mjwA
- active site: I333 (≠ A329), P377 (≠ V380), N378 (≠ C381), V464 (≠ I467), H466 (= H469), V509 (≠ G510), N510 (= N511)
- binding flavin-adenine dinucleotide: G20 (= G9), G22 (= G11), S23 (= S12), E44 (= E33), A45 (= A34), W71 (= W60), R89 (= R79), A90 (≠ G80), G95 (= G85), C96 (≠ S86), H99 (≠ L89), N100 (= N90), S101 (≠ G91), I103 (≠ L93), R231 (≠ L216), A232 (≠ V217), T269 (≠ G252), G270 (= G253), D273 (≠ N256), Y465 (= Y468), H466 (= H469), A500 (≠ C501), N510 (= N511), P511 (≠ T512), N512 (= N513), V515 (≠ A516)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
33% identity, 97% coverage: 2:527/540 of query aligns to 13:526/530 of 3ljpA
- active site: I333 (≠ A329), P377 (≠ V380), N378 (≠ C381), A464 (≠ I467), H466 (= H469), V509 (≠ G510), N510 (= N511)
- binding dihydroflavine-adenine dinucleotide: G22 (= G11), S23 (= S12), E44 (= E33), A45 (= A34), W71 (= W60), R89 (= R79), A90 (≠ G80), G95 (= G85), C96 (≠ S86), H99 (≠ L89), N100 (= N90), S101 (≠ G91), I103 (≠ L93), A232 (≠ V217), T269 (≠ G252), D273 (≠ N256), Y465 (= Y468), H466 (= H469), D499 (= D500), A500 (≠ C501), N510 (= N511), P511 (≠ T512), N512 (= N513), V515 (≠ A516)
5i68A Alcohol oxidase from pichia pastoris (see paper)
28% identity, 99% coverage: 2:538/540 of query aligns to 5:642/662 of 5i68A
- active site: H566 (= H469)
- binding flavin-adenine dinucleotide: G13 (≠ A10), G14 (= G11), S15 (= S12), S16 (≠ A13), E37 (= E33), A38 (= A34), M58 (= M53), A86 (≠ G80), G91 (= G85), G92 (≠ S86), N96 (= N90), F97 (≠ G91), M99 (≠ L93), K225 (≠ L216), V226 (= V217), C267 (≠ G252), G268 (= G253), S271 (≠ N256), W565 (≠ Y468), L605 (≠ C501), N615 (= N511), T616 (= T512), Y617 (≠ N513)
5hsaA Alcohol oxidase aox1 from pichia pastoris (see paper)
28% identity, 99% coverage: 2:538/540 of query aligns to 5:642/662 of 5hsaA
- active site: H566 (= H469)
- binding arabino-flavin-adenine dinucleotide: G14 (= G11), S15 (= S12), S16 (≠ A13), E37 (= E33), A38 (= A34), A86 (≠ G80), G92 (≠ S86), N96 (= N90), F97 (≠ G91), M99 (≠ L93), K225 (≠ L216), V226 (= V217), C267 (≠ G252), S271 (≠ N256), W565 (≠ Y468), L605 (≠ C501), N615 (= N511), T616 (= T512), Y617 (≠ N513)
Query Sequence
>3607390 FitnessBrowser__Dino:3607390
MEADYVVIGAGSAGCVVANRLSTDARNKVVLLEAGPPDTNPWIHIPVGYFKTMHNPTVDW
CYKTQADPGLNGRSIDWPRGKVLGGSSSLNGLLYVRGQPEDYDRWRQMGNAGWGWDDVLP
LFRRAEANERGADPWHGDDGPLAVSNMRIQRPICDAWVAAAQAMGYPFNPDYNGASQEGV
GYFQLTTRNGRRCSAAVAYLKPARKRPNLSIITRALVTRIEMEGKRVTGVTYTDAGGRAH
TVSARREVILSGGAINSPHILMLSGIGDPDQLQAHGITPRHALPGVGKNLQDHLQARLVF
KCNEPTLNDEVRSLVNQARIALKYALFRAGPMTMAASLATGFLKTRPDIATPDIQFHVQP
WSADSPGEGVHPFSAFTMSVCQLRPESRGEIRLAGPDPRTYPTIHPNYLSTETDCATLTE
GVKIARRIARADPLAGKIAEEFRPPANLALDDDAATLDWARSNSVSIYHPTGTCKMGTGP
GAVVDARLRVHGLSGLRVADCSIMPEIVSGNTNAPAIMIGEKLSDMVLEDARDTAQAVPA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory