SitesBLAST
Comparing 3607477 FitnessBrowser__Dino:3607477 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6rqaB Crystal structure of the iminosuccinate reductase of paracoccus denitrificans in complex with NAD+ (see paper)
61% identity, 97% coverage: 10:330/330 of query aligns to 3:322/322 of 6rqaB
- binding Tb-Xo4: E49 (= E56), D50 (= D57), N76 (= N83)
- binding nicotinamide-adenine-dinucleotide: T113 (= T120), G138 (= G145), Q140 (= Q147), P162 (= P169), H163 (= H170), I199 (= I206), T200 (= T207), S201 (= S208), S202 (≠ A209), M221 (= M228), G222 (= G229), D224 (= D231), K228 (= K235), G293 (= G301), T294 (= T302), G295 (= G303)
6rqaA Crystal structure of the iminosuccinate reductase of paracoccus denitrificans in complex with NAD+ (see paper)
61% identity, 97% coverage: 10:330/330 of query aligns to 3:322/322 of 6rqaA
- binding nicotinamide-adenine-dinucleotide: H85 (= H92), T113 (= T120), G138 (= G145), H139 (= H146), Q140 (= Q147), N161 (= N168), P162 (= P169), H163 (= H170), M166 (= M173), I199 (= I206), T200 (= T207), S201 (= S208), S202 (≠ A209), M221 (= M228), G222 (= G229), D224 (= D231), K228 (= K235), G293 (= G301)
A1B8Z0 Iminosuccinate reductase; EC 1.4.1.- from Paracoccus denitrificans (strain Pd 1222) (see paper)
61% identity, 97% coverage: 10:330/330 of query aligns to 1:320/320 of A1B8Z0
1omoA Alanine dehydrogenase dimer w/bound NAD (archaeal) (see paper)
32% identity, 97% coverage: 11:330/330 of query aligns to 4:319/320 of 1omoA
- active site: R52 (vs. gap), D219 (= D231)
- binding nicotinamide-adenine-dinucleotide: T109 (= T120), G134 (= G145), T135 (≠ H146), Q136 (= Q147), Y156 (≠ W167), D157 (≠ N168), V158 (≠ P169), R159 (≠ H170), T195 (= T207), P196 (≠ S208), G217 (= G229), D219 (= D231), K223 (= K235), S290 (≠ G301), T291 (= T302), G292 (= G303)
O28608 Alanine dehydrogenase; AlaDH; EC 1.4.1.1 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
32% identity, 97% coverage: 11:330/330 of query aligns to 4:319/322 of O28608
6t3eB Structure of thermococcus litoralis delta(1)-pyrroline-2-carboxylate reductase in complex with nadh and l-proline (see paper)
34% identity, 78% coverage: 73:330/330 of query aligns to 64:325/325 of 6t3eB
- binding 1,4-dihydronicotinamide adenine dinucleotide: S82 (≠ N91), T111 (= T120), G136 (= G145), V137 (≠ H146), Q138 (= Q147), D159 (≠ N168), I160 (≠ P169), A199 (≠ I206), T200 (= T207), T201 (≠ S208), A202 (= A209), V206 (≠ L213), V221 (≠ M228), G222 (= G229), W223 (≠ T230), S296 (≠ G301), V297 (≠ T302), G298 (= G303)
- binding proline: K67 (= K76), R110 (= R119)
Sites not aligning to the query:
4bvaA Crystal structure of the NADPH-t3 form of mouse mu-crystallin. (see paper)
34% identity, 67% coverage: 91:312/330 of query aligns to 81:293/303 of 4bvaA
- active site: S219 (≠ D231)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T106 (= T116), R109 (= R119), T110 (= T120), G135 (= G145), V136 (≠ H146), Q137 (= Q147), N158 (= N168), R159 (≠ P169), T160 (≠ H170), N163 (= N176), V194 (≠ I206), T195 (= T207), M196 (≠ S208), A197 (= A209), V216 (≠ M228), S282 (≠ G301), L283 (≠ T302), G284 (= G303)
- binding 3,5,3'triiodothyronine: S219 (≠ D231), R220 (≠ T232), W223 (≠ K235), E247 (= E261)
Sites not aligning to the query:
4bv9A Crystal structure of the NADPH form of mouse mu-crystallin. (see paper)
33% identity, 68% coverage: 89:312/330 of query aligns to 80:294/303 of 4bv9A
- active site: S220 (≠ D231)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: S82 (≠ N91), H83 (= H92), T111 (= T120), G134 (= G143), G136 (= G145), V137 (≠ H146), Q138 (= Q147), N159 (= N168), R160 (≠ P169), T161 (≠ H170), V195 (≠ I206), T196 (= T207), M197 (≠ S208), A198 (= A209), V217 (≠ M228), G218 (= G229), S283 (≠ G301), L284 (≠ T302), G285 (= G303)
- binding pyruvic acid: R110 (= R119)
Sites not aligning to the query:
Q14894 Ketimine reductase mu-crystallin; NADP-regulated thyroid-hormone-binding protein; EC 1.5.1.25 from Homo sapiens (Human) (see paper)
33% identity, 67% coverage: 91:312/330 of query aligns to 91:303/314 of Q14894
2i99A Crystal structure of human mu_crystallin at 2.6 angstrom (see paper)
33% identity, 67% coverage: 91:312/330 of query aligns to 90:302/312 of 2i99A
- active site: S228 (≠ D231)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: S90 (≠ N91), H91 (= H92), R118 (= R119), T119 (= T120), G142 (= G143), A143 (= A144), G144 (= G145), V145 (≠ H146), Q146 (= Q147), N167 (= N168), R168 (≠ P169), T169 (≠ H170), V203 (≠ I206), T204 (= T207), L205 (≠ S208), A206 (= A209), V225 (≠ M228), G226 (= G229), S291 (≠ G301), L292 (≠ T302), G293 (= G303)
Sites not aligning to the query:
Q9FLY0 Protein SAR DEFICIENT 4; Ornithine cyclodeaminase-like protein; AtOCD from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 75% coverage: 73:321/330 of query aligns to 68:323/325 of Q9FLY0
- G89 (≠ S94) mutation to E: In sard4-3; compromises systemic acquired resistance (SAR).
- G138 (= G143) mutation to D: In sard4-4; compromises systemic acquired resistance (SAR).
- S205 (≠ I204) mutation to N: In sard4-1; compromises systemic acquired resistance (SAR).
1u7hA Structure and a proposed mechanism for ornithine cyclodeaminase from pseudomonas putida (see paper)
30% identity, 75% coverage: 82:330/330 of query aligns to 74:321/341 of 1u7hA
- active site: D227 (= D231)
- binding nicotinamide-adenine-dinucleotide: T83 (≠ N91), R111 (= R119), T112 (= T120), G137 (= G145), A138 (≠ H146), Q139 (= Q147), D160 (≠ N168), T161 (≠ P169), V200 (≠ I206), T201 (= T207), A202 (≠ S208), I209 (≠ L213), V224 (≠ M228), G225 (= G229), D227 (= D231), K231 (= K235), S292 (≠ G301), V293 (≠ T302), G294 (= G303)
Sites not aligning to the query:
Q88H32 Ornithine cyclodeaminase; OCD; EC 4.3.1.12 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
30% identity, 75% coverage: 82:330/330 of query aligns to 75:322/350 of Q88H32
Sites not aligning to the query:
- 45 binding
- 69 binding
- 331 binding
1x7dA Crystal structure analysis of ornithine cyclodeaminase complexed with NAD and ornithine to 1.6 angstroms (see paper)
30% identity, 75% coverage: 82:330/330 of query aligns to 74:321/340 of 1x7dA
- active site: D227 (= D231)
- binding nicotinamide-adenine-dinucleotide: T83 (≠ N91), R111 (= R119), T112 (= T120), G137 (= G145), A138 (≠ H146), Q139 (= Q147), D160 (≠ N168), T161 (≠ P169), V200 (≠ I206), T201 (= T207), A202 (≠ S208), I209 (≠ L213), V224 (≠ M228), G225 (= G229), D227 (= D231), K231 (= K235), S292 (≠ G301), V293 (≠ T302), G294 (= G303)
- binding L-ornithine: R111 (= R119), D227 (= D231), V293 (≠ T302)
Sites not aligning to the query:
5gziA Cyclodeaminase_pa
27% identity, 96% coverage: 15:330/330 of query aligns to 25:334/354 of 5gziA
- binding nicotinamide-adenine-dinucleotide: Y85 (= Y80), T97 (≠ N91), R125 (= R119), T126 (= T120), G151 (= G145), A152 (≠ H146), Q153 (= Q147), D174 (≠ N168), T175 (≠ P169), H179 (≠ M173), A212 (≠ I206), T213 (= T207), S214 (= S208), V215 (≠ A209), V237 (≠ M228), G238 (= G229), A239 (≠ T230), S305 (≠ G301), T306 (= T302), G307 (= G303)
- binding (2S)-piperidine-2-carboxylic acid: R53 (= R50), K81 (= K76), R125 (= R119), A239 (≠ T230), T306 (= T302), G307 (= G303)
5gzlA Cyclodeaminase_pa
27% identity, 96% coverage: 15:330/330 of query aligns to 25:334/357 of 5gzlA
- binding lysine: G55 (≠ A52), E57 (≠ G54), I65 (≠ F62), E67 (≠ S64), D240 (= D231), R267 (≠ T258), E268 (≠ I259)
- binding nicotinamide-adenine-dinucleotide: Y85 (= Y80), T97 (≠ N91), I98 (≠ H92), T126 (= T120), G151 (= G145), A152 (≠ H146), Q153 (= Q147), D174 (≠ N168), T175 (≠ P169), H179 (≠ M173), A212 (≠ I206), T213 (= T207), S214 (= S208), V222 (≠ L213), V237 (≠ M228), G238 (= G229), A239 (≠ T230), D240 (= D231), K244 (= K235), S305 (≠ G301), T306 (= T302), G307 (= G303)
5yu4A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
27% identity, 96% coverage: 15:330/330 of query aligns to 21:330/344 of 5yu4A
- binding 2,4-diaminobutyric acid: E63 (≠ S64), K77 (= K76), R121 (= R119), T302 (= T302), G303 (= G303)
- binding nicotinamide-adenine-dinucleotide: Y81 (= Y80), T93 (≠ N91), I94 (≠ H92), R121 (= R119), T122 (= T120), G147 (= G145), A148 (≠ H146), Q149 (= Q147), D170 (≠ N168), T171 (≠ P169), H175 (≠ M173), A208 (≠ I206), T209 (= T207), S210 (= S208), V211 (≠ A209), V218 (≠ L213), V233 (≠ M228), A235 (≠ T230), S301 (≠ G301), T302 (= T302), G303 (= G303)
5yu3A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
27% identity, 96% coverage: 15:330/330 of query aligns to 21:330/344 of 5yu3A
- binding nicotinamide-adenine-dinucleotide: Y81 (= Y80), T93 (≠ N91), I94 (≠ H92), T122 (= T120), G147 (= G145), A148 (≠ H146), Q149 (= Q147), D170 (≠ N168), T171 (≠ P169), A208 (≠ I206), T209 (= T207), S210 (= S208), V211 (≠ A209), V233 (≠ M228), A235 (≠ T230), S301 (≠ G301), T302 (= T302), G303 (= G303)
- binding proline: M65 (≠ F66), K77 (= K76), R121 (= R119)
4mpdA Staphyloferrin b precursor biosynthetic enzyme sbnb bound a- ketoglutarate and NAD+ (see paper)
25% identity, 79% coverage: 69:330/330 of query aligns to 57:317/318 of 4mpdA
- active site: M224 (≠ Q236)
- binding 2-oxoglutaric acid: K64 (= K76), I66 (≠ G78), R80 (≠ H92), M289 (≠ T302)
- binding nicotinamide-adenine-dinucleotide (acidic form): R80 (≠ H92), R108 (= R119), T109 (= T120), G134 (= G145), I136 (≠ Q147), D158 (≠ N168), Q159 (≠ P169), F160 (≠ H170), T200 (= T207), V201 (≠ S208), I221 (≠ M228)
Sites not aligning to the query:
4mp6A Staphyloferrin b precursor biosynthetic enzyme sbnb bound to citrate and NAD+ (see paper)
25% identity, 78% coverage: 72:330/330 of query aligns to 72:333/334 of 4mp6A
- active site: M236 (≠ Q236)
- binding nicotinamide-adenine-dinucleotide: R92 (≠ H92), R120 (= R119), T121 (= T120), G146 (= G145), L147 (≠ H146), I148 (≠ Q147), D170 (≠ N168), Q171 (≠ P169), C211 (≠ I206), T212 (= T207), V213 (≠ S208), I233 (≠ M228), G306 (= G303)
Sites not aligning to the query:
Query Sequence
>3607477 FitnessBrowser__Dino:3607477
MLQKPDTDGLVIVTEEICQKVIDRDLAFAAVRDVFGAMARGDAYNFPVIREAIGHEDALY
GFKSGFDKAGMVLGVKAGGYWPNNMAKGIINHQSTVFLFDPDTGKLKALVGGNYLTAIRT
AASSAVSIDQLARKDAKVLGMVGAGHQSTFQLRAAVEQRDFEKVVCWNPHPDMIPNLGKV
AAEIGIPFEAVSREELGAQSDVIITITSAFEPLIEAGYIKPGTHLACMGTDTKGKQEVAA
ELVAKATLFTDEVAQSTTIGETQHAIASGAITQDDVTPIGKVINGDHPGRTSDDEITLFD
GTGVGLQDLAVAAVAAEQAVATGAATRVSL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory