SitesBLAST
Comparing 3607536 Dshi_0948 L-lactate dehydrogenase (cytochrome) (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P05414 Glycolate oxidase; GAO; GOX; Short chain alpha-hydroxy acid oxidase; EC 1.1.3.15 from Spinacia oleracea (Spinach) (see 4 papers)
36% identity, 96% coverage: 4:376/390 of query aligns to 3:354/369 of P05414
- Y24 (= Y25) mutation to F: 10-fold decrease in affinity for glycolate.
- PTA 77:79 (≠ PVG 78:80) binding
- S106 (= S107) binding
- W108 (≠ M109) mutation to S: 100-fold decrease in affinity for glycolate and 500-fold decrease in activity.
- QLY 127:129 (≠ QVY 128:130) binding
- T155 (= T156) binding
- K230 (= K252) binding
- S252 (= S274) binding
- DGGVR 285:289 (≠ DSGIR 307:311) binding
- GR 308:309 (= GR 330:331) binding
Sites not aligning to the query:
- 1 modified: N-acetylmethionine
6gmcA 1.2 a resolution structure of human hydroxyacid oxidase 1 bound with fmn and 4-carboxy-5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole
35% identity, 96% coverage: 1:374/390 of query aligns to 2:356/360 of 6gmcA
- active site: Y132 (= Y130), D160 (= D158), H258 (= H276)
- binding 5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole-4-carboxylate: Y26 (= Y25), M82 (≠ L81), W110 (≠ M109), Y132 (= Y130), R167 (= R165), F191 (≠ T189), L203 (= L221), Y206 (≠ W224), H258 (= H276), R261 (= R279)
- binding flavin mononucleotide: Y26 (= Y25), Y27 (≠ A26), A79 (≠ P78), T80 (≠ V79), A81 (≠ G80), S108 (= S107), Q130 (= Q128), Y132 (= Y130), T158 (= T156), K234 (= K252), H258 (= H276), G259 (= G277), R261 (= R279), D289 (= D307), G290 (≠ S308), R293 (= R311), G312 (= G330), R313 (= R331)
6gmbA Structure of human hydroxyacid oxidase 1 bound with fmn and glycolate
36% identity, 96% coverage: 1:374/390 of query aligns to 2:358/362 of 6gmbA
- active site: Y132 (= Y130), D160 (= D158), H260 (= H276)
- binding flavin mononucleotide: Y26 (= Y25), Y27 (≠ A26), A79 (≠ P78), T80 (≠ V79), A81 (≠ G80), S108 (= S107), Q130 (= Q128), Y132 (= Y130), K236 (= K252), H260 (= H276), G261 (= G277), R263 (= R279), D291 (= D307), G292 (≠ S308), G293 (= G309), R295 (= R311), G314 (= G330), R315 (= R331)
- binding glycolic acid: Y26 (= Y25), W110 (≠ M109), Y132 (= Y130), R167 (= R165), H260 (= H276), R263 (= R279)
Q9UJM8 2-Hydroxyacid oxidase 1; HAOX1; Glycolate oxidase; GO; GOX; Glyoxylate oxidase; EC 1.1.3.15; EC 1.2.3.5 from Homo sapiens (Human) (see 2 papers)
36% identity, 96% coverage: 1:374/390 of query aligns to 2:358/370 of Q9UJM8
2rduA Crystal structure of human glycolate oxidase in complex with glyoxylate (see paper)
36% identity, 96% coverage: 2:374/390 of query aligns to 1:356/360 of 2rduA
- active site: S106 (= S107), Y130 (= Y130), T156 (= T156), D158 (= D158), K234 (= K252), H258 (= H276)
- binding flavin mononucleotide: Y24 (= Y25), Y25 (≠ A26), A77 (≠ P78), T78 (≠ V79), A79 (≠ G80), S106 (= S107), Q128 (= Q128), Y130 (= Y130), T156 (= T156), K234 (= K252), H258 (= H276), G259 (= G277), R261 (= R279), D289 (= D307), G290 (≠ S308), G291 (= G309), R293 (= R311), G312 (= G330), R313 (= R331)
- binding glyoxylic acid: Y24 (= Y25), W108 (≠ M109), Y130 (= Y130), R165 (= R165), H258 (= H276), R261 (= R279)
5zzqA The crystal structure of mandelate oxidase with (s)-4-hydroxymandelic acid (see paper)
38% identity, 93% coverage: 7:368/390 of query aligns to 4:342/355 of 5zzqA
- active site: Y126 (= Y130), D154 (= D158), H250 (= H276)
- binding flavin mononucleotide: P75 (= P78), V76 (= V79), A77 (≠ G80), Q124 (= Q128), Y126 (= Y130), T152 (= T156), K226 (= K252), H250 (= H276), R253 (= R279), D281 (= D307), G283 (= G309), R285 (= R311), G304 (= G330), R305 (= R331)
- binding (2S)-hydroxy(4-hydroxyphenyl)ethanoic acid: L106 (≠ M109), Y126 (= Y130), M158 (≠ L162), R161 (= R165), T200 (= T225), F204 (= F229), H250 (= H276), R253 (= R279)
6a24A The crystal structure of mandelate oxidase with 3-fluoropyruvate (see paper)
38% identity, 93% coverage: 7:368/390 of query aligns to 4:342/354 of 6a24A
- active site: Y126 (= Y130), D154 (= D158), H250 (= H276)
- binding flavin mononucleotide: P75 (= P78), V76 (= V79), A77 (≠ G80), Q124 (= Q128), Y126 (= Y130), T152 (= T156), K226 (= K252), H250 (= H276), G251 (= G277), R253 (= R279), D281 (= D307), G282 (≠ S308), G283 (= G309), R285 (= R311), G304 (= G330), R305 (= R331)
- binding pyruvic acid: R161 (= R165), H250 (= H276), R253 (= R279)
5qigA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z1407672867
37% identity, 94% coverage: 7:374/390 of query aligns to 5:355/359 of 5qigA
- active site: Y129 (= Y130), D157 (= D158), H257 (= H276)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (≠ A26), A76 (≠ P78), T77 (≠ V79), A78 (≠ G80), S105 (= S107), Q127 (= Q128), Y129 (= Y130), T155 (= T156), K233 (= K252), H257 (= H276), G258 (= G277), R260 (= R279), D288 (= D307), G289 (≠ S308), G290 (= G309), R292 (= R311), G311 (= G330), R312 (= R331)
- binding 2-cyclopropyl-1~{H}-imidazole-4-carboxamide: R223 (= R242), G248 (= G267), F320 (≠ A339), K324 (≠ A343), D328 (≠ R347)
Sites not aligning to the query:
5qifA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z31792168
37% identity, 94% coverage: 7:374/390 of query aligns to 5:355/359 of 5qifA
- active site: Y129 (= Y130), D157 (= D158), H257 (= H276)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (≠ A26), A76 (≠ P78), T77 (≠ V79), A78 (≠ G80), S105 (= S107), Q127 (= Q128), Y129 (= Y130), K233 (= K252), H257 (= H276), G258 (= G277), R260 (= R279), D288 (= D307), G289 (≠ S308), R292 (= R311), G311 (= G330), R312 (= R331)
- binding 2-cyclohexyl-~{N}-pyridin-3-yl-ethanamide: R223 (= R242), R224 (≠ S243), S227 (≠ D246)
2rdwA Crystal structure of human glycolate oxidase in complex with sulfate (see paper)
37% identity, 94% coverage: 7:374/390 of query aligns to 5:355/359 of 2rdwA
- active site: S105 (= S107), Y129 (= Y130), T155 (= T156), D157 (= D158), K233 (= K252), H257 (= H276)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (≠ A26), A76 (≠ P78), T77 (≠ V79), A78 (≠ G80), Q127 (= Q128), Y129 (= Y130), T155 (= T156), K233 (= K252), H257 (= H276), G258 (= G277), R260 (= R279), D288 (= D307), G289 (≠ S308), R292 (= R311), G311 (= G330), R312 (= R331)
- binding sulfate ion: Y23 (= Y25), W107 (≠ M109), R164 (= R165), H257 (= H276), R260 (= R279)
1al7A Three-dimensional structures of glycolate oxidase with bound active- site inhibitors (see paper)
36% identity, 96% coverage: 4:376/390 of query aligns to 3:345/350 of 1al7A
- active site: S106 (= S107), Y129 (= Y130), T155 (= T156), D157 (= D158), K221 (= K252), H245 (= H276)
- binding flavin mononucleotide: Y24 (= Y25), Y25 (≠ A26), P77 (= P78), T78 (≠ V79), A79 (≠ G80), S106 (= S107), Q127 (= Q128), T155 (= T156), K221 (= K252), H245 (= H276), R248 (= R279), D276 (= D307), G277 (≠ S308), R280 (= R311), G299 (= G330), R300 (= R331)
- binding 4-carboxy-5-(1-pentyl)hexylsulfanyl-1,2,3-triazole: Y24 (= Y25), W108 (≠ M109), Y129 (= Y130), R164 (= R165), F172 (≠ L173), I198 (≠ F229), H245 (= H276), R248 (= R279)
7r4nA Structure of human hydroxyacid oxidase 1 bound with 5-bromo-n-methyl- 1h-indazole-3-carboxamide
35% identity, 96% coverage: 1:374/390 of query aligns to 1:348/352 of 7r4nA
- binding 5-bromanyl-N-methyl-1H-indazole-3-carboxamide: Y25 (= Y25), A80 (≠ G80), M81 (≠ L81), W109 (≠ M109), Y131 (= Y130), R166 (= R165), M182 (≠ P181), H250 (= H276), R253 (= R279)
- binding flavin mononucleotide: Y25 (= Y25), Y26 (≠ A26), A78 (≠ P78), T79 (≠ V79), A80 (≠ G80), S107 (= S107), W109 (≠ M109), Q129 (= Q128), Y131 (= Y130), T157 (= T156), K226 (= K252), H250 (= H276), G251 (= G277), R253 (= R279), D281 (= D307), G282 (≠ S308), R285 (= R311), G304 (= G330), R305 (= R331)
5qieA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z2856434894
36% identity, 94% coverage: 7:374/390 of query aligns to 5:349/353 of 5qieA
- active site: Y129 (= Y130), D157 (= D158), H251 (= H276)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (≠ A26), A76 (≠ P78), T77 (≠ V79), A78 (≠ G80), S105 (= S107), Q127 (= Q128), Y129 (= Y130), K227 (= K252), H251 (= H276), G252 (= G277), R254 (= R279), D282 (= D307), G283 (≠ S308), R286 (= R311), G305 (= G330), R306 (= R331)
- binding ~{N},~{N}-dimethyl-4-[(propan-2-ylamino)methyl]aniline: D322 (≠ R347)
Sites not aligning to the query:
5zbmB Structure of glycolate oxidase containing fmn from nicotiana benthamiana (see paper)
35% identity, 96% coverage: 4:377/390 of query aligns to 2:349/353 of 5zbmB
- active site: Y128 (= Y130), D156 (= D158), H248 (= H276)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (≠ A26), P76 (= P78), T77 (≠ V79), W107 (≠ M109), Q126 (= Q128), Y128 (= Y130), T154 (= T156), K224 (= K252), H248 (= H276), G249 (= G277), R251 (= R279), D279 (= D307), G280 (≠ S308), R283 (= R311), G302 (= G330), R303 (= R331)
P0DUR7 FMN-dependent alpha-hydroxy acid dehydrogenase qulF; Quinolactacin A2 biosynthesis cluster protein F; EC 1.13.12.- from Penicillium citrinum (see paper)
36% identity, 95% coverage: 1:372/390 of query aligns to 23:385/402 of P0DUR7
- H289 (= H276) mutation to A: Blocks the formatin of 2-aminobenzoylacetamide.
5qihA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z2697514548
35% identity, 94% coverage: 7:374/390 of query aligns to 5:340/344 of 5qihA
- active site: Y129 (= Y130), D157 (= D158), H242 (= H276)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (≠ A26), A76 (≠ P78), T77 (≠ V79), A78 (≠ G80), S105 (= S107), Q127 (= Q128), Y129 (= Y130), K218 (= K252), H242 (= H276), G243 (= G277), R245 (= R279), D273 (= D307), G274 (≠ S308), R277 (= R311), G296 (= G330), R297 (= R331)
- binding 1-methylindazole-3-carboxamide: Y23 (= Y25), A78 (≠ G80), M79 (≠ L81), W107 (≠ M109), Y129 (= Y130), M180 (≠ P181), L187 (= L221), H242 (= H276)
5qidA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z1787627869
35% identity, 94% coverage: 7:374/390 of query aligns to 5:340/344 of 5qidA
- active site: Y129 (= Y130), D157 (= D158), H242 (= H276)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (≠ A26), A76 (≠ P78), T77 (≠ V79), A78 (≠ G80), S105 (= S107), Q127 (= Q128), Y129 (= Y130), K218 (= K252), H242 (= H276), G243 (= G277), R245 (= R279), D273 (= D307), G274 (≠ S308), R277 (= R311), G296 (= G330), R297 (= R331)
- binding 5-chloranyl-~{N}-methyl-~{N}-[[(3~{S})-oxolan-3-yl]methyl]pyrimidin-4-amine: T57 (≠ R59), E320 (≠ K354), R323 (≠ D357)
5qicA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z30620520
35% identity, 94% coverage: 7:374/390 of query aligns to 5:340/344 of 5qicA
- active site: Y129 (= Y130), D157 (= D158), H242 (= H276)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (≠ A26), A76 (≠ P78), T77 (≠ V79), A78 (≠ G80), S105 (= S107), Q127 (= Q128), Y129 (= Y130), K218 (= K252), H242 (= H276), G243 (= G277), R245 (= R279), D273 (= D307), G274 (≠ S308), R277 (= R311), G296 (= G330), R297 (= R331)
- binding cyclopropyl-[4-(4-fluorophenyl)piperazin-1-yl]methanone: Y131 (≠ L132), K132 (= K133), V136 (≠ F137), M180 (≠ P181), F183 (≠ I184), Y190 (≠ W224), K193 (≠ E227), A194 (= A228)
5qibA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with fmopl000388a
35% identity, 94% coverage: 7:374/390 of query aligns to 5:340/344 of 5qibA
- active site: Y129 (= Y130), D157 (= D158), H242 (= H276)
- binding 1-deoxy-1-(7,8-dimethyl-2,4-dioxo-3,4-dihydro-2h-benzo[g]pteridin-1-id-10(5h)-yl)-5-o-phosphonato-d-ribitol: Y23 (= Y25), Y24 (≠ A26), A76 (≠ P78), T77 (≠ V79), A78 (≠ G80), S105 (= S107), Q127 (= Q128), Y129 (= Y130), K218 (= K252), H242 (= H276), G243 (= G277), R245 (= R279), D273 (= D307), G274 (≠ S308), G275 (= G309), R277 (= R311), G296 (= G330), R297 (= R331)
- binding 5-(cyclohexylamino)pyrimidine-2,4(1H,3H)-dione: Y23 (= Y25), M79 (≠ L81), W107 (≠ M109), Y129 (= Y130), R164 (= R165), M180 (≠ P181), L187 (= L221), H242 (= H276), R245 (= R279)
7r4oA Structure of human hydroxyacid oxidase 1 bound with 2-((4h-1,2,4- triazol-3-yl)thio)-1-(4-(3-chlorophenyl)piperazin-1-yl)ethan-1-one
35% identity, 96% coverage: 2:374/390 of query aligns to 1:337/341 of 7r4oA
- binding flavin mononucleotide: Y25 (≠ A26), A77 (≠ P78), T78 (≠ V79), A79 (≠ G80), S106 (= S107), Q128 (= Q128), Y130 (= Y130), K215 (= K252), H239 (= H276), G240 (= G277), R242 (= R279), D270 (= D307), G271 (≠ S308), R274 (= R311), G293 (= G330), R294 (= R331)
- binding 2-((4H-1,2,4-triazol-3-yl)thio)-1-(4-(3-chlorophenyl)piperazin-1-yl)ethan-1-one: Y132 (≠ L132), K133 (= K133), D134 (= D134), N183 (≠ S220), K190 (≠ E227)
Query Sequence
>3607536 Dshi_0948 L-lactate dehydrogenase (cytochrome) (RefSeq)
MPVITEIEDLKRIYKRRVPKMFYDYAESGSWTEQTFRENSSDFDLLKLRQRIAMDMDNRS
TKTTMVGQEVAMPVALAPVGLTGMQHADGEIKAARAAEKFGVPFTLSTMSICSIEDVAAH
TETPFWFQVYTLKDDDFMKRLFDRAKEAKCSALVITVDLQLLGQRHRDLKNGLSAPPKLT
PASIANMMTKVQWGLGMLGTKRRFFGNIVGHAKGVTDPSSLSSWTAEAFDQSLDWERIKQ
FRSWWDGPVILKGILDPEDAKMALNVGADAIVCSNHGGRQLDGALSSIRMLPQIMDAVGD
KIEVHLDSGIRSGQDVLKAVALGARGTMIGRAWTYGLGAMGEAGVTRALEVIHKELDLSM
GLCGRRSVEDLDASNLLIPEDFATRWKASA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory