SitesBLAST
Comparing 3607802 FitnessBrowser__Dino:3607802 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P19920 Carbon monoxide dehydrogenase medium chain; CO dehydrogenase subunit M; CO-DH M; EC 1.2.5.3 from Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) (Oligotropha carboxidovorans) (see 2 papers)
56% identity, 100% coverage: 1:284/284 of query aligns to 1:286/288 of P19920
1n5wC Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
56% identity, 100% coverage: 1:284/284 of query aligns to 1:286/287 of 1n5wC
1ffuC Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava which lacks the mo-pyranopterin moiety of the molybdenum cofactor (see paper)
49% identity, 100% coverage: 1:284/284 of query aligns to 1:286/287 of 1ffuC
- binding flavin-adenine dinucleotide: K29 (≠ R29), L30 (≠ C30), A32 (= A32), G33 (= G33), G34 (= G34), H35 (= H35), S36 (= S36), L37 (= L37), I54 (≠ L54), I101 (= I101), A102 (= A102), G110 (= G110), T111 (= T111), G114 (= G114), D115 (≠ N115), A117 (= A117), N123 (= N123), D124 (= D124), L161 (≠ R161), V166 (≠ I166), M167 (≠ L167), W193 (≠ Y191)
4zohB Crystal structure of glyceraldehyde oxidoreductase (see paper)
34% identity, 99% coverage: 1:281/284 of query aligns to 1:273/274 of 4zohB
- active site: Y256 (≠ F264)
- binding flavin-adenine dinucleotide: R28 (= R29), P29 (≠ C30), A31 (= A32), G32 (= G33), G33 (= G34), H34 (= H35), S35 (= S36), L36 (= L37), H77 (≠ Q78), I95 (= I101), G96 (≠ A102), V100 (= V106), T105 (= T111), G108 (= G114), S109 (≠ N115), S111 (≠ A117), A117 (≠ N123), D118 (= D124), L160 (≠ I166), V161 (≠ L167), D185 (= D190), F186 (≠ Y191)
7dqxE Crystal structure of xanthine dehydrogenase family protein
29% identity, 100% coverage: 1:284/284 of query aligns to 1:287/293 of 7dqxE
- binding flavin-adenine dinucleotide: K29 (≠ R29), I30 (≠ C30), I31 (≠ L31), G33 (= G33), G34 (= G34), Q35 (≠ H35), S36 (= S36), L37 (= L37), H78 (≠ Q78), I101 (= I101), A102 (= A102), I106 (≠ V106), G110 (= G110), T111 (= T111), G113 (= G113), G114 (= G114), S115 (≠ N115), A117 (= A117), A123 (≠ N123), E124 (≠ D124), I167 (≠ L167), Y193 (= Y191)
1t3qC Crystal structure of quinoline 2-oxidoreductase from pseudomonas putida 86 (see paper)
32% identity, 98% coverage: 4:280/284 of query aligns to 4:279/285 of 1t3qC
- binding flavin-adenine dinucleotide: I29 (≠ C30), A31 (= A32), G32 (= G33), G33 (= G34), Q34 (≠ H35), S35 (= S36), L36 (= L37), V100 (≠ I101), A101 (= A102), V105 (= V106), T110 (= T111), G113 (= G114), S114 (≠ N115), A116 (= A117), A122 (≠ N123), E123 (≠ D124), L166 (= L167), Y190 (= Y191)
Q0QLF4 Nicotinate dehydrogenase FAD-subunit; NDH; Nicotinic acid hydroxylase FAD-subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
28% identity, 94% coverage: 6:272/284 of query aligns to 4:279/296 of Q0QLF4
- 29:36 (vs. 30:37, 50% identical) binding
- G101 (≠ A102) binding
- TIGGN 110:114 (≠ TVGGN 111:115) binding
- D123 (= D124) binding
- R160 (= R161) binding
- M169 (≠ L167) binding
- K187 (≠ E182) binding
3hrdG Crystal structure of nicotinate dehydrogenase (see paper)
28% identity, 94% coverage: 6:272/284 of query aligns to 4:279/292 of 3hrdG
- binding flavin-adenine dinucleotide: I29 (≠ C30), A31 (= A32), G32 (= G33), G33 (= G34), T34 (≠ H35), D35 (≠ S36), F77 (≠ Q78), V100 (≠ I101), G101 (≠ A102), I105 (≠ V106), T110 (= T111), G113 (= G114), N114 (= N115), S116 (≠ A117), T117 (≠ N118), G122 (≠ N123), D123 (= D124), I168 (= I166), M169 (≠ L167)
2w54A Crystal structure of xanthine dehydrogenase from rhodobacter capsulatus in complex with bound inhibitor pterin-6-aldehyde (see paper)
30% identity, 86% coverage: 27:269/284 of query aligns to 186:427/450 of 2w54A
- binding flavin-adenine dinucleotide: L189 (≠ C30), A191 (= A32), G192 (= G33), G193 (= G34), T194 (≠ H35), D195 (vs. gap), V196 (vs. gap), W199 (≠ I38), L213 (= L51), F258 (≠ I101), A259 (= A102), A267 (≠ G110), T268 (= T111), G271 (= G114), N272 (= N115), A274 (= A117), N275 (= N118), G280 (≠ N123), D281 (= D124), R318 (= R161), V324 (≠ L167), Q347 (≠ G189)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 42
- binding fe2/s2 (inorganic) cluster: 38, 39, 40, 42, 44, 45, 47, 63, 103, 104, 106, 134, 135, 136
- binding {[(5aR,8R,9aR)-2-amino-4-oxo-6,7-di(sulfanyl-kappaS)-3,5,5a,8,9a,10-hexahydro-4H-pyrano[3,2-g]pteridin-8-yl]methyl dihydrogenato(2-) phosphate}(hydroxy)oxo(thioxo)molybdenum: 102, 136
1jroA Crystal structure of xanthine dehydrogenase from rhodobacter capsulatus (see paper)
30% identity, 86% coverage: 27:269/284 of query aligns to 186:427/450 of 1jroA
- binding flavin-adenine dinucleotide: L189 (≠ C30), A191 (= A32), G192 (= G33), G193 (= G34), T194 (≠ H35), D195 (vs. gap), V196 (vs. gap), F258 (≠ I101), A259 (= A102), T268 (= T111), G271 (= G114), N272 (= N115), A274 (= A117), G280 (≠ N123), D281 (= D124), R318 (= R161), V324 (≠ L167)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 42, 43
- binding fe2/s2 (inorganic) cluster: 37, 38, 39, 40, 42, 44, 45, 47, 63, 103, 106, 134, 136
O33820 4-hydroxybenzoyl-CoA reductase subunit beta; 4-HBCR subunit beta; EC 1.1.7.1 from Thauera aromatica (see paper)
26% identity, 97% coverage: 6:281/284 of query aligns to 7:321/324 of O33820
- 29:36 (vs. 30:37, 38% identical) binding
- T111 (= T111) binding
- N115 (= N115) binding
- Q118 (vs. gap) binding
- D162 (≠ N123) binding
- K224 (= K183) binding
1rm6B Structure of 4-hydroxybenzoyl-coa reductase from thauera aromatica (see paper)
26% identity, 97% coverage: 6:281/284 of query aligns to 7:321/323 of 1rm6B
- binding flavin-adenine dinucleotide: P29 (≠ C30), G31 (≠ A32), A32 (≠ G33), G33 (= G34), T34 (≠ H35), D35 (≠ S36), L36 (= L37), L53 (= L54), V101 (≠ I101), A102 (= A102), A110 (≠ G110), T111 (= T111), G114 (= G114), N115 (= N115), C117 (≠ A117), Q118 (vs. gap), D162 (≠ N123), L207 (= L167), V231 (≠ G189), D232 (= D190), F233 (≠ Y191)
- binding iron/sulfur cluster: C122 (vs. gap), F124 (vs. gap), C138 (vs. gap), C146 (vs. gap), H147 (vs. gap), V148 (vs. gap), C155 (vs. gap), Y156 (vs. gap), A157 (vs. gap)
5y6qB Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
22% identity, 96% coverage: 6:277/284 of query aligns to 4:323/330 of 5y6qB
- binding flavin-adenine dinucleotide: I26 (≠ C30), A28 (= A32), G29 (= G33), G30 (= G34), T31 (≠ H35), T32 (≠ S36), Q33 (≠ L37), A97 (≠ I101), A98 (= A102), I102 (≠ V106), A106 (≠ G110), T107 (= T111), G110 (= G114), N111 (= N115), R113 (vs. gap), Q114 (vs. gap), G164 (vs. gap), D165 (= D124), I213 (= I166), I214 (≠ L167), K232 (≠ E182), F241 (≠ Y191)
- binding iron/sulfur cluster: C118 (vs. gap), Y121 (vs. gap), C130 (vs. gap), N131 (≠ A117), C139 (vs. gap), A141 (vs. gap), C158 (vs. gap), I159 (vs. gap), A160 (vs. gap)
6a7xA Rat xanthine oxidoreductase, d428a variant, NAD bound form
25% identity, 61% coverage: 93:266/284 of query aligns to 298:481/1295 of 6a7xA
- binding flavin-adenine dinucleotide: F309 (vs. gap), V314 (= V106), S319 (≠ T111), G322 (= G114), N323 (= N115), I325 (≠ A117), T326 (≠ N118), D332 (= D124), L376 (= L167)
- binding nicotinamide-adenine-dinucleotide: P329 (= P121), Y365 (≠ A156), R366 (≠ Y157), A432 (≠ S218), D433 (= D219), P473 (≠ R259), R480 (≠ K265)
Sites not aligning to the query:
- active site: 740, 775, 853, 857, 885, 1233, 1234
- binding bicarbonate ion: 812, 813, 850, 883, 884, 887, 888, 891
- binding flavin-adenine dinucleotide: 44, 228, 229, 230, 231, 232, 233, 234, 235, 236
- binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 69, 71, 109, 110, 111, 113, 145, 147
- binding uric acid: 775, 853, 887, 982, 983, 1052, 1234
6a7xB Rat xanthine oxidoreductase, d428a variant, NAD bound form
25% identity, 61% coverage: 93:266/284 of query aligns to 296:479/1291 of 6a7xB
- binding flavin-adenine dinucleotide: F307 (vs. gap), V312 (= V106), S317 (≠ T111), G320 (= G114), N321 (= N115), I323 (≠ A117), T324 (≠ N118), D330 (= D124), I373 (= I166), L374 (= L167)
- binding nicotinamide-adenine-dinucleotide: P327 (= P121), Y363 (≠ A156), R364 (≠ Y157), G428 (≠ N216), A430 (≠ S218), D431 (= D219), P471 (≠ R259), R478 (≠ K265)
Sites not aligning to the query:
- active site: 738, 773, 851, 855, 883, 1231, 1232
- binding bicarbonate ion: 810, 811, 880, 881, 885, 886, 889
- binding flavin-adenine dinucleotide: 44, 227, 228, 229, 230, 231, 232, 233, 234
- binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 69, 71, 109, 110, 111, 113, 145, 147, 715
- binding uric acid: 773, 851, 885, 980, 981, 1049, 1050, 1232
P22985 Xanthine dehydrogenase/oxidase; EC 1.17.1.4; EC 1.17.3.2 from Rattus norvegicus (Rat) (see 2 papers)
25% identity, 61% coverage: 93:266/284 of query aligns to 325:508/1331 of P22985
- WF 335:336 (vs. gap) mutation to AL: Converts the enzyme to the oxidase form that utilizes molecular oxygen as electron acceptor. Interferes with normal conversion to the dehydrogenase form by reducing agents.
- SIGGN 346:350 (≠ TVGGN 111:115) binding
- D359 (= D124) binding
- L403 (= L167) binding
Sites not aligning to the query:
- 43 binding
- 48 binding
- 51 binding
- 73 binding
- 112 binding
- 115 binding
- 147 binding
- 149 binding
- 256:263 binding
- 535 C→A: Slows the conversion from the dehydrogenase form to the oxidase form; when associated with R-992. Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with R-992 and S-1316.
- 992 C→R: Slows the conversion from the dehydrogenase form to the oxidase form; when associated with A-535. Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with A-535 and S-1316.
- 1316 C→S: Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with A-535 and R-992.
2e3tA Crystal structure of rat xanthine oxidoreductase mutant (w335a and f336l) (see paper)
25% identity, 60% coverage: 96:266/284 of query aligns to 304:481/1291 of 2e3tA
- binding flavin-adenine dinucleotide: L309 (≠ I101), A318 (≠ G110), S319 (≠ T111), G322 (= G114), N323 (= N115), I325 (≠ A117), T326 (≠ N118), S331 (≠ N123), D332 (= D124), I375 (= I166), L376 (= L167)
Sites not aligning to the query:
- active site: 740, 775, 853, 857, 885, 1233, 1234
- binding bicarbonate ion: 812, 813, 850, 882, 883, 887, 888, 891
- binding calcium ion: 713, 714, 716, 809, 810, 840, 843, 844, 847, 880, 881
- binding flavin-adenine dinucleotide: 44, 228, 229, 230, 231, 232, 233, 234, 235, 236
- binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 69, 71, 109, 110, 113, 145, 147, 717
- binding uric acid: 775, 853, 887, 982, 983, 1051, 1052, 1234
4yswA Structure of rat xanthine oxidoreductase, c-terminal deletion protein variant, nadh bound form (see paper)
25% identity, 61% coverage: 93:266/284 of query aligns to 296:479/1286 of 4yswA
- binding flavin-adenine dinucleotide: F307 (vs. gap), A308 (= A102), V312 (= V106), A316 (≠ G110), S317 (≠ T111), G320 (= G114), N321 (= N115), I323 (≠ A117), T324 (≠ N118), S329 (≠ N123), D330 (= D124), I373 (= I166), L374 (= L167), D399 (≠ G189)
- binding 1,4-dihydronicotinamide adenine dinucleotide: S326 (≠ D120), P327 (= P121), I328 (≠ G122), Y363 (≠ A156), R364 (≠ Y157), D400 (= D190), I401 (≠ Y191), G428 (≠ N216), A430 (≠ S218), D431 (= D219), P471 (≠ R259), R478 (≠ K265)
Sites not aligning to the query:
- active site: 738, 773, 851, 855, 883, 1231, 1232
- binding bicarbonate ion: 810, 811, 848, 880, 881, 882, 885, 886, 889
- binding calcium ion: 838, 841, 842, 845, 878, 879
- binding flavin-adenine dinucleotide: 44, 226, 227, 228, 229, 230, 231, 232, 233, 234
- binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 71, 110, 111, 113, 145, 147, 715
- binding 1,4-dihydronicotinamide adenine dinucleotide: 233, 1196
- binding uric acid: 773, 851, 885, 980, 981, 1050, 1232
Q8GUQ8 Xanthine dehydrogenase 1; AtXDH1; EC 1.17.1.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
22% identity, 91% coverage: 8:266/284 of query aligns to 264:539/1361 of Q8GUQ8
- W364 (vs. gap) mutation to A: Decreases activity 8-fold.
- Y421 (≠ A156) mutation to A: Decreases activity 4-fold.
Sites not aligning to the query:
- 831 E→A: Loss of activity.
- 909 R→A: Decreases activity 12-fold.
- 1297 E→A: Decreases activity 40-fold.
P47989 Xanthine dehydrogenase/oxidase; EC 1.17.1.4; EC 1.17.3.2 from Homo sapiens (Human) (see 4 papers)
23% identity, 86% coverage: 21:265/284 of query aligns to 248:508/1333 of P47989
- 257:264 (vs. 30:37, 13% identical) binding
- F337 (vs. gap) binding
- SVGGN 347:351 (≠ TVGGN 111:115) binding
- D360 (= D124) binding
- K395 (≠ T158) to M: in dbSNP:rs34929837
- K422 (≠ E182) binding
Sites not aligning to the query:
- 133 E → K: in dbSNP:rs45447191
- 172 G → R: in dbSNP:rs45523133
- 235 T → M: in dbSNP:rs45469499
- 509 modified: Disulfide link with 1318, In oxidase form
- 536 modified: Disulfide link with 993, In oxidase form
- 555 P → S: in dbSNP:rs45577338
- 584 D → A: in dbSNP:rs45491693
- 607 R → Q: in dbSNP:rs45442092
- 617 K → N: in dbSNP:rs45442398
- 623 T → I: in dbSNP:rs45448694
- 646 I → V: in dbSNP:rs17323225
- 703 I → V: in dbSNP:rs17011368
- 763 L → F: in a breast cancer sample; somatic mutation
- 791 R → G: in a breast cancer sample; somatic mutation; dbSNP:rs775646772
- 910 T → M: in dbSNP:rs669884
- 993 modified: Disulfide link with 536, In oxidase form
- 1091 V → L: in dbSNP:rs45619033
- 1109 N → T: in dbSNP:rs45547640
- 1150 P → R: in dbSNP:rs1042036
- 1176 R → C: in dbSNP:rs45624433
- 1296 R → W: in dbSNP:rs45564939
- 1318 modified: Disulfide link with 509, In oxidase form
Query Sequence
>3607802 FitnessBrowser__Dino:3607802
MIPPAFEYHRPTDLASAVSVLQEHGDEARCLAGGHSLIPMMKLRMAAVEHLVDLQDIAEL
REIRVEGGRVTLGAMVTQHALITSDALASAAPLLREAAEQIADPQVRYMGTVGGNVANGD
PGNDMPGLMQALDASFTLMGPDGTREVAARDFFEGAYTTTREDEEILTQIAFDAHAKGYA
YEKQKRKIGDYATAAAAVLIEKDGTVAGKVDIAMTNLSDTPVYSAAAGAALVGTSCDAEA
VKAAVTAMLEDIDPMPDNRGPVEFKRHVAGVILGRAIARAWSRA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory