SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3607803 FitnessBrowser__Dino:3607803 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P19921 Carbon monoxide dehydrogenase small chain; CO dehydrogenase subunit S; CO-DH S; EC 1.2.5.3 from Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) (Oligotropha carboxidovorans) (see 2 papers)
66% identity, 100% coverage: 1:163/163 of query aligns to 1:166/166 of P19921

query
sites
P19921

M

M

S

A

K

K

M

A

H

H

I

I

K

E

M

L

T

T

V

I

N

N

G

G

K

H

P

P

E

V

E

E

M

A

L

L

A

V

E

E

P

P

R

R

E

T

L

L

I

I

Y

H

T

F

L

I

R

R

E

E

R

Q

L

Q

N

N

I

L

T

T

G

G

P

A

H

H

V

I

G

G

C
|
C

E

D

T

T

S

S

H

H

C
|
C

G

G

A

A

C
|
C

T

T

V

V

E

D

M

L

D

D

G

G

K

M

S

S

V

V

K

K

A

S

C
|
C

T

T

V

M

F

F

A

A

A

V

Q

Q

A

A

H

N

G

G

A

A

E

S

I

I

T

T

V

I

E

E

G

G

L

M

G

A

D

A

P

P

D

D

-

G

N

T

L

L

H

S

V

A

L

L

Q

Q

R

E

M

G

F

F

K

R

E

M

H

M

H

H

G

G

L

L

Q

Q

C
|
C

G

G

Y

Y

C
|
C

T

T

P

P

G

G

M

M

I

I

T

M

R

R

A

S

H

H

R

R

L

L

Q

Q

E

E

N

N

P

P

N

S

P

P

T

T

E

E

E

A

E

E

V

I

R

R

F

F

G

G

M

I

A

G

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

Q

N

N

I

I

V

V

K

K

S

A

I

I

L

Q

A

Y

A

A

E

K

M

I

N

N

S

G

A

V

-

P

-

F

K

E

E

E

A

A

E

E

C42 (= C42) binding
C47 (= C47) binding
C50 (= C50) binding
C62 (= C62) binding
C102 (= C101) binding
C105 (= C104) binding
C137 (= C136) binding
C139 (= C138) binding

1n5wA Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
67% identity, 94% coverage: 3:156/163 of query aligns to 1:155/161 of 1n5wA

query
sites
1n5wA

K

K

M

A

H

H

I

I

K

E

M

L

T

T

V

I

N

N

G

G

K

H

P

P

E

V

E

E

M

A

L

L

A

V

E

E

P

P

R

R

E

T

L

L

I

I

Y

H

T

F

L

I

R

R

E

E

R

Q

L

Q

N

N

I

L

T

T

G

G

P

A

H

H

V
x
I

G
|
G

C
|
C

E

D

T

T

S
|
S

H
|
H

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

E

D

M

L

D

D

G

G

K

M

S

S

V

V

K

K

A

S

C
|
C

T

T

V

M

F

F

A

A

A

V

Q

Q

A

A

H

N

G

G

A

A

E

S

I

I

T

T

V

I

E

E

G

G

L

M

G

A

D

A

P

P

D

D

-

G

N

T

L

L

H

S

V

A

L

L

Q

Q

R

E

M

G

F

F

K

R

E

M

H

M

H

H

G

G

L

L

Q

Q

C
|
C

G
|
G

Y

Y

C
|
C

T

T

P

P

G

G

M

M

I

I

T

M

R

R

A

S

H

H

R

R

L

L

Q

Q

E

E

N

N

P

P

N

S

P

P

T

T

E

E

E

A

E

E

V

I

R

R

F

F

G

G

M

I

A

G

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

Q

N

N

I

I

V

V

K

K

S

A

I

I

L

Q

A

Y

A

A

E

K

M

I

N

N

binding flavin-adenine dinucleotide: S43 (= S45), H44 (= H46)
binding fe2/s2 (inorganic) cluster: I38 (≠ V40), G39 (= G41), C40 (= C42), S43 (= S45), C45 (= C47), G46 (= G48), C48 (= C50), C60 (= C62), C100 (= C101), G101 (= G102), C103 (= C104), C135 (= C136), C137 (= C138)

1n5wD Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
67% identity, 94% coverage: 3:156/163 of query aligns to 1:155/158 of 1n5wD

query
sites
1n5wD

K

K

M

A

H

H

I

I

K

E

M

L

T

T

V

I

N

N

G

G

K

H

P

P

E

V

E

E

M

A

L

L

A

V

E

E

P

P

R

R

E

T

L

L

I

I

Y

H

T

F

L

I

R

R

E

E

R

Q

L

Q

N

N

I

L

T

T

G

G

P

A

H

H

V

I

G

G

C
|
C

E

D

T

T

S
|
S

H
|
H

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

E

D

M

L

D

D

G

G

K

M

S

S

V

V

K

K

A

S

C
|
C

T

T

V

M

F

F

A

A

A

V

Q

Q

A

A

H

N

G

G

A

A

E

S

I

I

T

T

V

I

E

E

G

G

L

M

G

A

D

A

P

P

D

D

-

G

N

T

L

L

H

S

V

A

L

L

Q

Q

R

E

M

G

F

F

K

R

E

M

H

M

H

H

G

G

L

L

Q
|
Q

C
|
C

G
|
G

Y

Y

C
|
C

T

T

P

P

G

G

M

M

I

I

T

M

R

R

A

S

H

H

R

R

L

L

Q

Q

E

E

N

N

P

P

N

S

P

P

T

T

E

E

E

A

E

E

V

I

R

R

F

F

G

G

M

I

A

G

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

Q

N

N

I

I

V

V

K

K

S

A

I

I

L

Q

A

Y

A

A

E

K

M

I

N

N

binding flavin-adenine dinucleotide: S43 (= S45), H44 (= H46)
binding fe2/s2 (inorganic) cluster: C40 (= C42), S43 (= S45), C45 (= C47), G46 (= G48), C48 (= C50), C60 (= C62), C100 (= C101), G101 (= G102), C103 (= C104), C135 (= C136), C137 (= C138)
binding pterin cytosine dinucleotide: Q99 (= Q100), C137 (= C138)

1ffuD Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava which lacks the mo-pyranopterin moiety of the molybdenum cofactor (see paper)
62% identity, 94% coverage: 2:155/163 of query aligns to 1:154/156 of 1ffuD

query
sites
1ffuD

S

A

K

K

M

K

H

I

I

I

K

T

M

V

T

N

V

V

N

N

G

G

K

K

P

A

E

Q

E

E

M

K

L

A

A

V

E

E

P

P

R

R

E

T

L

L

I

I

Y

H

T

F

L

L

R

R

E

E

R

E

L

L

N

N

I

L

T

T

G

G

P

A

H

H

V

I

G

G

C
|
C

E

E

T

T

S
|
S

H
|
H

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

E

D

M

I

D

D

G

G

K

R

S

S

V

V

K

K

A

S

C
|
C

T

T

V

H

F

L

A

A

A

V

Q

Q

A

C

H

D

G

G

A

S

E

E

I

V

T

L

T

T

V

V

E

E

G

G

L

L

G

A

D

N

P

K

D

G

N

V

L

L

H

H

V

A

L

V

Q

Q

R

E

M

G

F

F

K

Y

E

K

H

E

H

H

G

G

L

L

Q

Q

C
|
C

G
|
G

Y

F

C
|
C

T

T

P

P

G

G

M

M

I

L

T

M

R

R

A

A

H

Y

R

R

L

F

L

L

Q

Q

E

E

N

N

P

P

N

N

P

P

T

T

E

E

E

A

E

E

V

I

R

R

F

M

G

G

M

M

A

T

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

Q

N

N

I

I

V

V

K

K

S

A

I

V

L

Q

A

Y

A

A

A

R

E

K

M

L

binding fe2/s2 (inorganic) cluster: C41 (= C42), S44 (= S45), H45 (= H46), C46 (= C47), G47 (= G48), C49 (= C50), C61 (= C62), C100 (= C101), G101 (= G102), C103 (= C104), C135 (= C136), C137 (= C138)

1ffvA Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava (see paper)
63% identity, 94% coverage: 3:155/163 of query aligns to 1:153/155 of 1ffvA

query
sites
1ffvA

K

K

M

K

H

I

I

I

K

T

M

V

T

N

V

V

N

N

G

G

K

K

P

A

E

Q

E

E

M

K

L

A

A

V

E

E

P

P

R

R

E

T

L

L

I

I

Y

H

T

F

L

L

R

R

E

E

R

E

L

L

N

N

I

L

T

T

G

G

P

A

H

H

V
x
I

G

G

C
|
C

E

E

T

T

S
|
S

H

H

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

E

D

M

I

D

D

G

G

K

R

S

S

V

V

K

K

A

S

C
|
C

T

T

V

H

F

L

A

A

A

V

Q

Q

A

C

H

D

G

G

A

S

E

E

I

V

T

L

T

T

V

V

E

E

G

G

L

L

G

A

D

N

P

K

D

G

N

V

L

L

H

H

V

A

L

V

Q

Q

R

E

M

G

F

F

K

Y

E

K

H

E

H

H

G

G

L

L

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

T

T

P

P

G

G

M

M

I

L

T

M

R

R

A

A

H

Y

R

R

L

F

L

L

Q

Q

E

E

N

N

P

P

N

N

P

P

T

T

E

E

E

A

E

E

V

I

R

R

F

M

G

G

M

M

A

T

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

Q

N

N

I

I

V

V

K

K

S

A

I

V

L

Q

A

Y

A

A

A

R

E

K

M

L

binding fe2/s2 (inorganic) cluster: I38 (≠ V40), C40 (= C42), S43 (= S45), C45 (= C47), G46 (= G48), C48 (= C50), C60 (= C62), C99 (= C101), G100 (= G102), C102 (= C104), C134 (= C136), C136 (= C138)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q98 (= Q100), C136 (= C138)

4zohC Crystal structure of glyceraldehyde oxidoreductase (see paper)
62% identity, 94% coverage: 3:156/163 of query aligns to 9:161/161 of 4zohC

query
sites
4zohC

K

K

M

V

H

K

I

I

K

T

M

L

T

K

V

I

N

N

G

G

K

E

P

K

E

Y

E

E

M

T

L

E

A

V

E

E

P

P

R

R

E

R

L

L

I

V

Y

H

T

V

L

L

R

R

E

E

R

-

L

L

N

G

I

F

T

T

G

G

P

V

H

H

V

I

G

G

C
|
C

E

D

T

T

S
|
S

H

N

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

E

I

M

M

D

N

G

G

K

K

S

S

V

V

K

K

A

S

C
|
C

T

T

V

V

F

L

A

A

A

V

Q

E

A

A

H

D

G

G

A

A

E

E

I

I

T

L

T

T

V

V

E

E

G

G

L

L

G

A

D

K

P

D

D

G

N

K

L

L

H

H

V

P

L

I

Q

Q

R

E

M

A

F

F

K

W

E

E

H

N

H

H

G

A

L

L

Q
|
Q

C
|
C

G
|
G

Y

Y

C
|
C

T

T

P

P

G

G

M

M

I

I

T

M

R

E

A

A

H

Y

R

W

L

L

Q

R

E

E

N

K

P

P

N

N

P

P

T

T

E

E

V

I

R

R

F

E

G

G

M

I

A

S

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

Q

N

N

I

I

V

V

K

K

S

A

I

I

L

K

A

A

A

E

E

K

M

L

N

S

binding fe2/s2 (inorganic) cluster: C47 (= C42), S50 (= S45), C52 (= C47), G53 (= G48), C55 (= C50), C67 (= C62), C106 (= C101), G107 (= G102), C109 (= C104), C141 (= C136), C143 (= C138)
binding pterin cytosine dinucleotide: Q105 (= Q100), C143 (= C138)

1t3qA Crystal structure of quinoline 2-oxidoreductase from pseudomonas putida 86 (see paper)
49% identity, 89% coverage: 4:148/163 of query aligns to 4:148/162 of 1t3qA

query
sites
1t3qA

M

M

H

R

I

I

K

S

M

A

T

T

V

I

N

N

G

G

K

K

P

P

E

R

E

V

M

F

L

Y

A

V

E

E

P

P

R

R

E

M

L

H

L

L

I

A

Y

D

T

A

L

L

R

R

E

E

R

V

L

V

N

G

I

L

T

T

G

G

P

T

H

K

V
x
I

G

G

C
|
C

E
|
E

T

Q

S
x
G

H

V

C
|
C

G
|
G

A

S

C
|
C

T

T

V

I

E

L

M

I

D

D

G

G

K

A

S

P

V

M

K
x
R

A

S

C
|
C

T

L

V

T

F

L

A

A

A

V

Q

Q

A

A

H

E

G

G

A

C

E

S

I

I

T

E

T

T

V

V

E

E

G

G

L

L

G

S

D

Q

P

G

D

E

N

K

L

L

H

N

V

A

L

L

Q

Q

R

D

M

S

F

F

K

R

E

R

H

H

G

A

L

L

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

T

T

P

A

G

G

M

M

I

L

T

A

R

T

A

A

H

R

R

S

L

I

L

L

Q

A

E

E

N

N

P

P

N

A

P

P

T

S

E

R

E

D

E

E

V

V

R

R

F

E

G

V

M

M

A

S

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

E

N

T

I

I

V

I

K

D

S

A

I

I

binding fe2/s2 (inorganic) cluster: I40 (≠ V40), C42 (= C42), E43 (= E43), G45 (≠ S45), C47 (= C47), G48 (= G48), C50 (= C50), R60 (≠ K60), C62 (= C62), C101 (= C101), G102 (= G102), C104 (= C104), C136 (= C136), C138 (= C138)
binding pterin cytosine dinucleotide: Q100 (= Q100), C138 (= C138)

4usaA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with trans-cinnamaldehyde (see paper)
47% identity, 93% coverage: 8:159/163 of query aligns to 6:160/907 of 4usaA

query
sites
4usaA

M

I

T

T

V

V

N

N

G

G

K

I

P

E

E

Q

E

N

M

L

L

F

A

V

E

D

P

A

R

E

E

A

L

L

I

S

Y

D

T

V

L

L

R

R

E

Q

R

Q

L

L

N

G

I

L

T

T

G

G

P

V

H

K

V
|
V

G

G

C
|
C

E
|
E

T

Q

S
x
G

H

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

V

V

E

I

M

L

D

D

G

G

K

K

S

V

V

V

K
x
R

A

A

C
|
C

-

V

T

T

V

K

F

M

A

K

A

R

Q

V

A

A

H

D

G

G

A

A

E

Q

I

I

T

T

V

I

E

E

G

G

L

V

G

G

D

Q

P

P

D

E

N

N

L

L

H

H

V

P

L

L

Q

Q

R

K

M

A

F

W

K

V

E

L

H

H

H

G

G

G

L

A

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

T

S

P

P

G

G

M

F

I

I

T

V

R

S

A

A

H

K

R

G

L

L

Q

D

E

T

N

N

P

A

N

D

P

P

T

S

E

R

E

E

E

D

V

V

R

R

-

D

-

W

F

F

G

Q

M

K

A

H

G

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

K

N

P

I

L

V

V

K

D

S

A

I

V

L

M

A

D

A

A

E

V

M

I

N

N

S

G

A

K

K

K

binding fe2/s2 (inorganic) cluster: V38 (= V40), C40 (= C42), E41 (= E43), G43 (≠ S45), C45 (= C47), G46 (= G48), C48 (= C50), R58 (≠ K60), C60 (= C62), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C139 (= C138)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding bicarbonate ion: 460, 531, 532, 535, 539
binding hydrocinnamic acid: 255, 425, 494, 497, 535, 626
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

4us9A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with 3- phenylpropionaldehyde (see paper)
47% identity, 93% coverage: 8:159/163 of query aligns to 6:160/907 of 4us9A

query
sites
4us9A

M

I

T

T

V

V

N

N

G

G

K

I

P

E

E

Q

E

N

M

L

L

F

A

V

E

D

P

A

R

E

E

A

L

L

I

S

Y

D

T

V

L

L

R

R

E

Q

R

Q

L

L

N

G

I

L

T

T

G

G

P

V

H

K

V
|
V

G

G

C
|
C

E
|
E

T

Q

S
x
G

H

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

V

V

E

I

M

L

D

D

G

G

K

K

S

V

V

V

K
x
R

A

A

C
|
C

-

V

T

T

V

K

F

M

A

K

A

R

Q

V

A

A

H

D

G

G

A

A

E

Q

I

I

T

T

V

I

E

E

G

G

L

V

G

G

D

Q

P

P

D

E

N

N

L

L

H

H

V

P

L

L

Q

Q

R

K

M

A

F

W

K

V

E

L

H

H

H

G

G

G

L

A

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

T

S

P

P

G

G

M

F

I

I

T

V

R

S

A

A

H

K

R

G

L

L

Q

D

E

T

N

N

P

A

N

D

P

P

T

S

E

R

E

E

E

D

V

V

R

R

-

D

-

W

F

F

G

Q

M

K

A

H

G

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

K

N

P

I

L

V

V

K

D

S

A

I

V

L

M

A

D

A

A

E

V

M

I

N

N

S

G

A

K

K

K

binding fe2/s2 (inorganic) cluster: V38 (= V40), C40 (= C42), E41 (= E43), G43 (≠ S45), C45 (= C47), G46 (= G48), C48 (= C50), R58 (≠ K60), C60 (= C62), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C139 (= C138)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding 3-phenylpropanal: 255, 257, 258, 752
binding bicarbonate ion: 460, 498, 531, 532, 535, 539, 890, 892
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

4us8A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with benzaldehyde (see paper)
47% identity, 93% coverage: 8:159/163 of query aligns to 6:160/907 of 4us8A

query
sites
4us8A

M

I

T

T

V

V

N

N

G

G

K

I

P

E

E

Q

E

N

M

L

L

F

A

V

E

D

P

A

R

E

E

A

L

L

I

S

Y

D

T

V

L

L

R

R

E

Q

R

Q

L

L

N

G

I

L

T

T

G

G

P

V

H

K

V
|
V

G

G

C
|
C

E
|
E

T

Q

S
x
G

H

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

V

V

E

I

M

L

D

D

G

G

K

K

S

V

V

V

K
x
R

A

A

C
|
C

-

V

T

T

V

K

F

M

A

K

A

R

Q

V

A

A

H

D

G

G

A

A

E

Q

I

I

T

T

V

I

E

E

G

G

L

V

G

G

D

Q

P

P

D

E

N

N

L

L

H

H

V

P

L

L

Q

Q

R

K

M

A

F

W

K

V

E

L

H

H

H

G

G

G

L

A

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

T

S

P

P

G

G

M

F

I

I

T

V

R

S

A

A

H

K

R

G

L

L

Q

D

E

T

N

N

P

A

N

D

P

P

T

S

E

R

E

E

E

D

V

V

R

R

-

D

-

W

F

F

G

Q

M

K

A

H

G

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

K

N

P

I

L

V

V

K

D

S

A

I

V

L

M

A

D

A

A

E

V

M

I

N

N

S

G

A

K

K

K

binding fe2/s2 (inorganic) cluster: V38 (= V40), C40 (= C42), E41 (= E43), G43 (≠ S45), C45 (= C47), G46 (= G48), C48 (= C50), R58 (≠ K60), C60 (= C62), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C139 (= C138)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding bicarbonate ion: 460, 498, 531, 532, 535, 539
binding benzaldehyde: 255, 255, 394, 425, 425, 425, 425, 497, 497, 501, 531, 535, 535, 626, 626, 626, 694, 696, 697
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

4c7yA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with sodium dithionite and sodium sulfide (see paper)
47% identity, 93% coverage: 8:159/163 of query aligns to 6:160/907 of 4c7yA

query
sites
4c7yA

M

I

T

T

V

V

N

N

G

G

K

I

P

E

E

Q

E

N

M

L

L

F

A

V

E

D

P

A

R

E

E

A

L

L

I

S

Y

D

T

V

L

L

R

R

E

Q

R

Q

L

L

N

G

I

L

T

T

G

G

P

V

H

K

V

V

G

G

C
|
C

E
|
E

T

Q

S
x
G

H

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

V

V

E

I

M

L

D

D

G

G

K

K

S

V

V

V

K
x
R

A

A

C
|
C

-

V

T

T

V

K

F

M

A

K

A

R

Q

V

A

A

H

D

G

G

A

A

E

Q

I

I

T

T

V

I

E

E

G

G

L

V

G

G

D

Q

P

P

D

E

N

N

L

L

H

H

V

P

L

L

Q

Q

R

K

M

A

F

W

K

V

E

L

H

H

H

G

G

G

L

A

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

T

S

P

P

G

G

M

F

I

I

T

V

R

S

A

A

H

K

R

G

L

L

Q

D

E

T

N

N

P

A

N

D

P

P

T

S

E

R

E

E

E

D

V

V

R

R

-

D

-

W

F

F

G

Q

M

K

A

H

G

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

K

N

P

I

L

V

V

K

D

S

A

I

V

L

M

A

D

A

A

E

V

M

I

N

N

S

G

A

K

K

K

binding fe2/s2 (inorganic) cluster: C40 (= C42), E41 (= E43), G43 (≠ S45), C45 (= C47), G46 (= G48), C48 (= C50), R58 (≠ K60), C60 (= C62), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C139 (= C138)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding bicarbonate ion: 460, 498, 531, 535, 539
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
binding hydrogen peroxide: 696, 697, 869

3fc4A Ethylene glycol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
47% identity, 93% coverage: 8:159/163 of query aligns to 6:160/907 of 3fc4A

query
sites
3fc4A

M

I

T

T

V

V

N

N

G

G

K

I

P

E

E

Q

E

N

M

L

L

F

A

V

E

D

P

A

R

E

E

A

L

L

I

S

Y

D

T

V

L

L

R

R

E

Q

R

Q

L

L

N

G

I

L

T

T

G

G

P

V

H

K

V
|
V

G

G

C
|
C

E
|
E

T

Q

S
x
G

H

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

V

V

E

I

M

L

D

D

G

G

K

K

S

V

V

V

K
x
R

A

A

C
|
C

-

V

T

T

V

K

F

M

A

K

A

R

Q

V

A

A

H

D

G

G

A

A

E

Q

I

I

T

T

V

I

E

E

G

G

L

V

G

G

D

Q

P

P

D

E

N

N

L

L

H

H

V

P

L

L

Q

Q

R

K

M

A

F

W

K

V

E

L

H

H

H

G

G

G

L

A

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

T

S

P

P

G

G

M

F

I

I

T

V

R

S

A

A

H

K

R

G

L

L

Q

D

E

T

N

N

P

A

N

D

P

P

T

S

E

R

E

E

E

D

V

V

R

R

-

D

-

W

F

F

G

Q

M

K

A

H

G

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

K

N

P

I

L

V

V

K

D

S

A

I

V

L

M

A

D

A

A

E

V

M

I

N

N

S

G

A

K

K

K

binding fe2/s2 (inorganic) cluster: V38 (= V40), C40 (= C42), E41 (= E43), G43 (≠ S45), C45 (= C47), G46 (= G48), C48 (= C50), R58 (≠ K60), C60 (= C62), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C139 (= C138)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding 1,2-ethanediol: 535, 622, 696, 697, 869
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

3fahA Glycerol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
47% identity, 93% coverage: 8:159/163 of query aligns to 6:160/907 of 3fahA

query
sites
3fahA

M

I

T

T

V

V

N

N

G

G

K

I

P

E

E

Q

E

N

M

L

L

F

A

V

E

D

P

A

R

E

E

A

L

L

I

S

Y

D

T

V

L

L

R

R

E

Q

R

Q

L

L

N

G

I

L

T

T

G

G

P

V

H

K

V
|
V

G

G

C
|
C

E
|
E

T

Q

S
x
G

H

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

V

V

E

I

M

L

D

D

G

G

K

K

S

V

V

V

K
x
R

A

A

C
|
C

-

V

T

T

V

K

F

M

A

K

A

R

Q

V

A

A

H

D

G

G

A

A

E

Q

I

I

T

T

V

I

E

E

G

G

L

V

G

G

D

Q

P

P

D

E

N

N

L

L

H

H

V

P

L

L

Q

Q

R

K

M

A

F

W

K

V

E

L

H

H

H

G

G

G

L

A

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

T

S

P

P

G

G

M

F

I

I

T

V

R

S

A

A

H

K

R

G

L

L

Q

D

E

T

N

N

P

A

N

D

P

P

T

S

E

R

E

E

E

D

V

V

R

R

-

D

-

W

F

F

G

Q

M

K

A

H

G

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

K

N

P

I

L

V

V

K

D

S

A

I

V

L

M

A

D

A

A

E

V

M

I

N

N

S

G

A

K

K

K

binding fe2/s2 (inorganic) cluster: V38 (= V40), C40 (= C42), E41 (= E43), G43 (≠ S45), C45 (= C47), G46 (= G48), C48 (= C50), R58 (≠ K60), C60 (= C62), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C139 (= C138)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding glycerol: 416, 535, 622, 683, 696, 697, 869, 884, 889, 890, 892
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

1sijA Crystal structure of the aldehyde dehydrogenase (a.K.A. Aor or mop) of desulfovibrio gigas covalently bound to [aso3]- (see paper)
47% identity, 93% coverage: 8:159/163 of query aligns to 6:160/907 of 1sijA

query
sites
1sijA

M

I

T

T

V

V

N

N

G

G

K

I

P

E

E

Q

E

N

M

L

L

F

A

V

E

D

P

A

R

E

E

A

L

L

I

S

Y

D

T

V

L

L

R

R

E

Q

R

Q

L

L

N

G

I

L

T

T

G

G

P

V

H

K

V
|
V

G

G

C
|
C

E
|
E

T

Q

S
x
G

H

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

V

V

E

I

M

L

D

D

G

G

K

K

S

V

V

V

K
x
R

A

A

C
|
C

-

V

T

T

V

K

F

M

A

K

A

R

Q

V

A

A

H

D

G

G

A

A

E

Q

I

I

T

T

V

I

E

E

G

G

L

V

G

G

D

Q

P

P

D

E

N

N

L

L

H

H

V

P

L

L

Q

Q

R

K

M

A

F

W

K

V

E

L

H

H

H

G

G

G

L

A

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

T

S

P

P

G

G

M

F

I

I

T

V

R

S

A

A

H

K

R

G

L

L

Q

D

E

T

N

N

P

A

N

D

P

P

T

S

E

R

E

E

E

D

V

V

R

R

-

D

-

W

F

F

G

Q

M

K

A

H

G

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

K

N

P

I

L

V

V

K

D

S

A

I

V

L

M

A

D

A

A

E

V

M

I

N

N

S

G

A

K

K

K

binding fe2/s2 (inorganic) cluster: V38 (= V40), C40 (= C42), E41 (= E43), G43 (≠ S45), C45 (= C47), G46 (= G48), C48 (= C50), R58 (≠ K60), C60 (= C62), Q99 (= Q100), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C139 (= C138)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding arsenite: 535, 696, 697, 869
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 698, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

Q46509 Aldehyde oxidoreductase; Molybdenum iron sulfur protein; EC 1.2.99.7 from Megalodesulfovibrio gigas (Desulfovibrio gigas) (see paper)
47% identity, 93% coverage: 8:159/163 of query aligns to 6:160/907 of Q46509

query
sites
Q46509

M

I

T

T

V

V

N

N

G

G

K

I

P

E

E

Q

E

N

M

L

L

F

A

V

E

D

P

A

R

E

E

A

L

L

I

S

Y

D

T

V

L

L

R

R

E

Q

R

Q

L

L

N

G

I

L

T

T

G

G

P

V

H

K

V

V

G

G

C
|
C

E

E

T

Q

S

G

H

Q

C
|
C

G

G

A

A

C
|
C

T

S

V

V

E

I

M

L

D

D

G

G

K

K

S

V

V

V

K

R

A

A

C
|
C

-

V

T

T

V

K

F

M

A

K

A

R

Q

V

A

A

H

D

G

G

A

A

E

Q

I

I

T

T

V

I

E

E

G

G

L

V

G

G

D

Q

P

P

D

E

N

N

L

L

H

H

V

P

L

L

Q

Q

R

K

M

A

F

W

K

V

E

L

H

H

H

G

G

G

L

A

Q

Q

C
|
C

G

G

Y

F

C
|
C

T

S

P

P

G

G

M

F

I

I

T

V

R

S

A

A

H

K

R

G

L

L

Q

D

E

T

N

N

P

A

N

D

P

P

T

S

E

R

E

E

E

D

V

V

R

R

-

D

-

W

F

F

G

Q

M

K

A

H

G

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

K

N

P

I

L

V

V

K

D

S

A

I

V

L

M

A

D

A

A

E

V

M

I

N

N

S

G

A

K

K

K

C40 (= C42) binding
C45 (= C47) binding
C48 (= C50) binding
C60 (= C62) binding
C100 (= C101) binding
C103 (= C104) binding
C137 (= C136) binding
C139 (= C138) binding

5y6qA Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
47% identity, 94% coverage: 3:155/163 of query aligns to 2:155/157 of 5y6qA

query
sites
5y6qA

K

R

M

I

H

S

I

L

K

T

M

M

T

Q

V

V

N

N

G

G

K

Q

P

P

E

Y

E

P

M

M

L

E

A

I

E

D

P

P

R

R

E

V

L

T

L

L

I

L

Y

D

T

A

L

L

R

R

E

E

R

H

L

L

N

N

I

L

T

T

G

G

P

T

H

K

V

K

G
|
G

C
|
C

E
x
D

T

Q

S
x
G

H

Q

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

E

L

M

V

D

N

G

G

K

Q

S

R

V

V

K

L

A

S

C
|
C

T

L

V

T

F

L

A

A

Q

Q

A

A

H

D

G

G

A

A

E

E

I

V

T

T

T

S

V

I

E

E

G

G

L

L

G

Q

D

S

P

A

D

S

N

G

-

E

L

L

H

H

V

P

L

V

Q

Q

R

R

M

C

F

F

K

V

E

E

H

N

H

D

G

A

L

F

Q
|
Q

C
|
C

G
|
G

Y

Y

C
|
C

T

T

P

P

G

G

M

Q

I

I

T

M

R

S

A

A

H

V

R

A

L

C

L

I

Q

K

E

E

N

G

P

H

N

A

P

G

T

S

E

D

E

E

V

I

R

R

F

E

G

Y

M

M

A

S

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

G

G

A

Y

Y

Q

P

N

H

I

I

V

I

K

D

S

A

I

I

L

K

A

Q

A

A

E

E

M

M

binding fe2/s2 (inorganic) cluster: G40 (= G41), C41 (= C42), D42 (≠ E43), G44 (≠ S45), C46 (= C47), G47 (= G48), C49 (= C50), C61 (= C62), C101 (= C101), G102 (= G102), C104 (= C104), C136 (= C136), C138 (= C138)
binding pterin cytosine dinucleotide: Q100 (= Q100), C138 (= C138)

P77165 Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA; EC 1.2.99.6 from Escherichia coli (strain K12) (see paper)
43% identity, 94% coverage: 4:157/163 of query aligns to 61:229/229 of P77165

query
sites
P77165

M

M

H

P

I

L

K

T

M

L

T

K

V

V

N

N

G

G

K

K

P

T

E

E

E

Q

M

L

L

E

A

V

E

D

P

T

R

R

E

T

L

T

L

L

I

L

Y

D

T

T

L

L

R

R

E

E

R

N

L

L

N

H

I

L

T

I

G

G

P

T

H

K

V

K

G

G

C
|
C

E

D

T

H

S

G

H

Q

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

E

L

M

V

D

N

G

G

K

R

S

R

V

L

K

N

A

A

C
|
C

T

L

V

T

F

L

A

A

A

V

Q

M

A

H

H

Q

G

G

A

A

E

E

I

I

T

T

V

I

E

E

G

G

L

L

G

G

D

S

P

P

D

D

N

N

L

L

H

H

V

P

L

M

Q

Q

R

A

M

A

F

F

K

I

E

K

H

H

H

D

G

G

L

F

Q

Q

C
|
C

G

G

Y

Y

C
|
C

T

T

P

S

G

G

M

Q

I

I

T

C

R

S

A

S

H

V

R

A

L

V

L

L

Q

K

E

E

-

I

-

Q

-

D

-

G

-

I

-

P

-

S

-

H

-

V

-

T

-

V

-

D

-

L

-

V

-

S

N

A

P

P

N

E

P

T

T

T

E

A

E

D

E

E

V

I

R

R

F

E

G

R

M

M

A

S

G

G

N

N

L

I

C
|
C

R

R

C
|
C

T

G

G

A

Y

Y

Q

A

N

N

I

I

V

L

K

A

S

A

I

I

L

E

A

D

A

A

A

G

E

E

M

I

N

K

S

S

C99 (= C42) binding
C104 (= C47) binding
G105 (= G48) binding
C107 (= C50) binding
C119 (= C62) binding
C158 (= C101) binding
C161 (= C104) binding
C208 (= C136) binding
C210 (= C138) binding

1dgjA Crystal structure of the aldehyde oxidoreductase from desulfovibrio desulfuricans atcc 27774 (see paper)
45% identity, 96% coverage: 8:163/163 of query aligns to 6:164/906 of 1dgjA

query
sites
1dgjA

M

L

T

I

V

V

N

N

G

G

K

M

P

A

E

R

M

L

L

L

A

V

E

S

P

P

R

N

E

D

L

L

I

V

Y

D

T

V

L

L

R

R

E

S

R

Q

L

L

N

Q

I

L

T

T

G

S

P

V

H

K

V
|
V

G
|
G

C
|
C

E
x
G

T

K

S
x
G

H
x
Q

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

E

I

M

L

D

D

G

G

K

K

S

V

V

V

K
x
R

A

A

C
|
C

T

I

V

I

F

K

A

M

A

S

Q

R

-

V

A

A

H

E

G

N

A

A

E

S

I

V

T

T

V

L

E

E

G

G

L

I

G

G

D

A

P

P

D

D

N

C

L

L

H

H

V

P

L

L

Q

Q

R

H

M

A

F

W

K

I

E

Q

H

H

H

G

G

A

L

A

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

T

T

P

P

G

G

M

F

I

I

T

V

R

S

A

A

H

K

R

A

L

L

Q

D

E

E

N

N

P

V

N

A

P

P

T

S

E

R

E

E

E

D

V

V

R

R

-

D

-

W

F

F

G

Q

M

K

A

H

G

H

N

N

L

I

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

K

N

P

I

L

V

V

K

D

S

A

I

V

L

M

A

D

A

A

E

I

M

L

N

R

S

G

A

E

K

K

E

T

A

V

A

E

E

E

binding fe2/s2 (inorganic) cluster: V38 (= V40), G39 (= G41), C40 (= C42), G41 (≠ E43), G43 (≠ S45), Q44 (≠ H46), C45 (= C47), G46 (= G48), C48 (= C50), R58 (≠ K60), C60 (= C62), C100 (= C101), G101 (= G102), C103 (= C104), C137 (= C136), C139 (= C138)
binding pterin cytosine dinucleotide: Q99 (= Q100), C139 (= C138)

Sites not aligning to the query:

active site: 391, 427, 503, 507, 535, 869, 870
binding molybdenum (iv)oxide: 424, 535, 698, 869
binding pterin cytosine dinucleotide: 423, 424, 535, 652, 655, 656, 657, 658, 697, 698, 700, 702, 703, 799, 800, 803, 804, 807, 865, 866, 867, 868, 869

1sb3C Structure of 4-hydroxybenzoyl-coa reductase from thauera aromatica (see paper)
45% identity, 93% coverage: 3:153/163 of query aligns to 2:152/161 of 1sb3C

query
sites
1sb3C

K

K

M

N

H

I

I

L

K

R

M

L

T

T

V

L

N

N

G

G

K

R

P

A

E

R

E

E

M

D

L

L

A

V

E

P

P

D

R

N

E

M

L

L

I

L

Y

D

T

Y

L

L

R

R

E

E

R

T

L

V

N

G

I

L

T

T

G

G

P

T

H

K

V
x
Q

G

G

C
|
C

E

D

T

G

S
x
G

H

E

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

E

L

M

V

D

D

G

D

K

R

S

P

V

R

K

L

A

A

C
|
C

T

S

V

T

F

L

A

A

A

H

Q

Q

A

V

H

A

G

G

A

K

E

K

I

V

T

E

T

T

V

V

E

E

G

S

L

L

G

A

D

T

P

Q

D

G

N

T

L

L

H

S

V

K

L

L

Q

Q

R

A

M

A

F

F

K

H

E

E

H

K

H

L

G

G

L

T

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

T

T

P

P

G

G

M

M

I

I

T

M

R

A

A

S

H

E

R

A

L

L

Q

R

E

K

N

N

P

P

N

S

P

P

T

S

E

R

E

D

E

E

V

I

R

K

F

A

G

A

M

L

A

A

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

V

N

K

I

I

V

I

K

K

S

S

I

V

L

E

A

T

A

A

binding fe2/s2 (inorganic) cluster: Q39 (≠ V40), C41 (= C42), G44 (≠ S45), C46 (= C47), G47 (= G48), C49 (= C50), C61 (= C62), C100 (= C101), G101 (= G102), C103 (= C104), C135 (= C136), C137 (= C138)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q100), C137 (= C138)

5g5gA Escherichia coli periplasmic aldehyde oxidase (see paper)
43% identity, 93% coverage: 4:154/163 of query aligns to 10:175/175 of 5g5gA

query
sites
5g5gA

M

M

H

P

I

L

K

T

M

L

T

K

V

V

N

N

G

G

K

K

P

T

E

E

E

Q

M

L

L

E

A

V

E

D

P

T

R

R

E

T

L

T

L

L

I

L

Y

D

T

T

L

L

R

R

E

E

R

N

L

L

N

H

I

L

T

I

G

G

P

T

H

K

V

K

G
|
G

C
|
C

E
x
D

T

H

S
x
G

H

Q

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

E

L

M

V

D

N

G

G

K

R

S

R

V

L

K

N

A

A

C
|
C

T

L

V

T

F

L

A

A

A

V

Q

M

A

H

H

Q

G

G

A

A

E

E

I

I

T

T

V

I

E

E

G

G

L

L

G

G

D

S

P

P

D

D

N

N

L

L

H

H

V

P

L

M

Q

Q

R

A

M

A

F

F

K

I

E

K

H

H

H

D

G

G

L

F

Q

Q

C
|
C

G
|
G

Y

Y

C
|
C

T

T

P

S

G

G

M

Q

I

I

T

C

R

S

A

S

H

V

R

A

L

V

L

L

Q

K

E

E

-

I

-

Q

-

D

-

G

-

I

-

P

-

S

-

H

-

V

-

T

-

V

-

D

-

L

-

V

-

S

N

A

P

P

N

E

P

T

T

T

E

A

E

D

E

E

V

I

R

R

F

E

G

R

M

M

A

S

G

G

N

N

L

I

C
|
C

R

R

C
|
C

T

G

G

A

Y

Y

Q

A

N

N

I

I

V

L

K

A

S

A

I

I

L

E

A

D

A

A

A

G

E

E

binding fe2/s2 (inorganic) cluster: G47 (= G41), C48 (= C42), D49 (≠ E43), G51 (≠ S45), C53 (= C47), G54 (= G48), C56 (= C50), C68 (= C62), C107 (= C101), G108 (= G102), C110 (= C104), C157 (= C136), C159 (= C138)

Query Sequence

>3607803 FitnessBrowser__Dino:3607803
MSKMHIKMTVNGKPEEMLAEPRELLIYTLRERLNITGPHVGCETSHCGACTVEMDGKSVK
ACTVFAAQAHGAEITTVEGLGDPDNLHVLQRMFKEHHGLQCGYCTPGMITRAHRLLQENP
NPTEEEVRFGMAGNLCRCTGYQNIVKSILAAAAEMNSAKEAAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory