SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3607850 Dshi_1258 short-chain dehydrogenase/reductase SDR (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
40% identity, 94% coverage: 8:239/247 of query aligns to 11:251/255 of 5itvA

query
sites
5itvA

L

L

I

I

T

T

G
|
G

G

G

A
|
A

Q
x
S

G
|
G

I
|
I

G

G

Y

Y

A

A

C

A

A

V

E

Q

A

A

L

F

A

L

A

G

E

Q

G

Q

A

A

R

N

I

V

I

V

L

V

A

A

D
|
D

I
|
I

Q

D

D

E

S

A

V

Q

Q

G

E

E

A

A

A

M

A

V

R

R

L

K

G

E

G

N

A

N

G

D

Y

R

L

L

-

H

-

F

-

V

-

Q

C
x
T

D
|
D

M
x
I

G

T

D

D

A

E

A

A

A

A

V

C

G

Q

A

H

L

A

F

V

D

E

R

S

I

A

E

V

A

H

E

T

H

F

G

G

A

G

V

L

T

D

Y

V

L

L

V

I

N

N

N
|
N

A
|
A

G
|
G

I
|
I

A

E

A

I

P

V

G

A

D

P

F

I

L

H

D

E

Y

M

D

E

L

L

A

S

T

D

F

W

D

N

R

K

V

V

L

L

D

Q

I
x
V

N

N

L

L

R

T

G

G

V

M

F

F

V

L

A

M

T

S

Q

K

R

H

A

A

G

L

R

K

S

H

M

M

V

L

A

A

H

A

G

G

I

-

K

K

G

G

A

N

V

I

V

I

N

N

M
x
T

S
x
C

S
|
S

I

V

N

G

A

G

Q

L

V

V

A

A

I

W

P

P

S

D

I

I

P

P

A

A

Y
|
Y

C

N

A

A

S

S

K
|
K

G

G

G

G

L

V

M

L

Q

Q

L

L

T

T

K

K

V

S

A

M

A

A

L

V

A

D

L

Y

A

A

P

K

H

H

G

Q

I

I

R

R

V

V

N

N

A

C

V

V

G

C

P
|
P

G
|
G

S
x
I

I
|
I

D

D

T
|
T

E

P

M
x
L

-
x
N

-

E

M

K

A

S

G

F

V

L

N

E

A

N

N

N

P

E

E

G

A

T

M

L

N

E

M

E

V

I

L

K

S

K

R

E

-

K

-

A

-

K

-

V

T

N

P

P

L

L

K

L

R

R

V

L

G

G

T

K

A

P

Q

E

E

E

I

I

A

A

N

N

V

V

V

M

A

L

F

F

L

L

C

A

S

S

D

D

K

L

A

S

S

S

Y

Y

V

M

T

T

G

G

E

S

T

A

I

I

Y

T

V

A

D

D

G

G

G

G

active site: G18 (= G15), S141 (= S135), Y154 (= Y148), K158 (= K152)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G11), A16 (= A13), S17 (≠ Q14), G18 (= G15), I19 (= I16), D38 (= D35), I39 (= I36), T61 (≠ C54), D62 (= D55), I63 (≠ M56), N89 (= N82), A90 (= A83), G91 (= G84), I92 (= I85), V112 (≠ I105), T139 (≠ M133), C140 (≠ S134), S141 (= S135), Y154 (= Y148), K158 (= K152), P184 (= P178), G185 (= G179), I186 (≠ S180), I187 (= I181), T189 (= T183), L191 (≠ M185), N192 (vs. gap)

1g6kA Crystal structure of glucose dehydrogenase mutant e96a complexed with NAD+
37% identity, 95% coverage: 6:239/247 of query aligns to 9:249/261 of 1g6kA

query
sites
1g6kA

L

V

A

V

L

V

I

I

T

T

G
|
G

G

S

A

S

Q
x
T

G
|
G

I
x
L

G

G

Y

K

A

S

C

M

A

A

E

I

A

R

L

F

A

A

A

T

E

E

G

K

A

A

R

K

I

V

I

V

L

V

-
x
N

-

Y

-
x
R

-

S

-

K

A

E

D

D

I

E

Q

A

D

N

S

S

V

V

Q

L

E

E

A

E

A

I

A

K

R

K

L

V

G

G

G

G

A

E

G

A

Y

-

L

I

C

A

D

V

M

K

G
|
G

D
|
D

A
x
V

A

T

A

V

V

E

G

S

A

D

L

V

F

I

D

N

R

L

I

V

E

Q

A

S

-

A

-

I

-

K

E

E

H

F

G

G

A

K

V

L

T

D

Y

V

L

M

V

I

N

N

N
|
N

A
|
A

G
|
G

I

L

A

A

A

N

P

P

G

V

D

S

F

S

L

H

D

E

Y

M

D

S

L

L

A

S

T

D

F

W

D

N

R

K

V

V

L

I

D

D

I
x
T

N

N

L

L

R

T

G

G

V

A

F

F

V

L

A

G

T

S

Q

R

R

E

A

A

G

I

R

K

S

Y

M

F

V

V

A

E

H

N

G

D

I

I

K

K

G

G

A

T

V

V

V

I

N

N

M
|
M

S
|
S

S
|
S

I

V

N

H

A

E

Q

K

V

I

A

P

I

W

P

P

S

L

I

F

P

V

A

H

Y
|
Y

C

A

A

A

S

S

K
|
K

G

G

G

G

L

M

M

K

Q

L

L

M

T

T

K

E

V

T

A

L

A

A

L

L

A

E

L

Y

A

A

P

P

H

K

G

G

I

I

R

R

V

V

N

N

A

N

V

I

G

G

P
|
P

G
|
G

S
x
A

I
|
I

D

N

T
|
T

E

P

M
x
I

M
x
N

A

A

G

E

V

K

N

F

A

A

N

D

P

P

E

E

A

Q

M

R

N

A

M

D

V

V

L

E

S

S

R

M

T

I

P

P

L

M

K

G

R

Y

V

I

G

G

T

E

A

P

Q

E

E

E

I

I

A

A

N

A

V

V

V

A

A

A

F

W

L

L

C

A

S

S

D

S

K

E

A

A

S

S

Y

Y

V

V

T

T

G

G

E

I

T

T

I

L

Y

F

V

A

D

D

G

G

G

G

active site: G18 (= G15), S145 (= S135), Y158 (= Y148), K162 (= K152)
binding nicotinamide-adenine-dinucleotide: G14 (= G11), T17 (≠ Q14), G18 (= G15), L19 (≠ I16), N37 (vs. gap), R39 (vs. gap), G64 (= G57), D65 (= D58), V66 (≠ A59), N92 (= N82), A93 (= A83), G94 (= G84), T115 (≠ I105), M143 (= M133), S144 (= S134), S145 (= S135), Y158 (= Y148), K162 (= K152), P188 (= P178), G189 (= G179), A190 (≠ S180), I191 (= I181), T193 (= T183), I195 (≠ M185), N196 (≠ M186)

P40288 Glucose 1-dehydrogenase; EC 1.1.1.47 from Priestia megaterium (Bacillus megaterium) (see 2 papers)
36% identity, 95% coverage: 6:239/247 of query aligns to 9:249/261 of P40288

query
sites
P40288

L

V

A

V

L
x
V

I
|
I

T
|
T

G
|
G

G
x
S

A
x
S

Q
x
T

G
|
G

I
x
L

G
|
G

Y
x
K

A
x
S

C
x
M

A
|
A

E
x
I

A
x
R

L
x
F

A
|
A

A
x
T

E
|
E

G
x
K

A
|
A

R
x
K

I
x
V

I
x
V

L

V

-

N

-

Y

-

R

-

S

-

K

A

E

D

D

I

E

Q

A

D

N

S

S

V

V

Q

L

E

E

A

E

A

I

A

K

R

K

L

V

G

G

G

G

A

E

G

A

Y

-

L

I

C

A

D

V

M

K

G

G

D

D

A

V

A

T

A

V

V

E

G

S

A

D

L

V

F

I

D

N

R

L

I

V

E

Q

A

S

-

A

-

I

-

K

E

E

H

F

G

G

A

K

V

L

T

D

Y

V

L

M

V

I

N

N

N

N

A

A

G

G

I

L

A
x
E

A

N

P

P

G

V

D

S

F

S

L

H

D

E

Y

M

D

S

L

L

A

S

T
x
D

F

W

D

N

R

K

V
|
V

L

I

D

D

I

T

N

N

L

L

R

T

G

G

V

A

F

F

V

L

A

G

T

S

Q

R

R

E

A

A

G

I

R

K

S

Y

M

F

V

V

A
x
E

H

N

G

D

I

I

K

K

G

G

A

T

V

V

V

I

N

N

M

M

S

S

S

S

I

V

N

H

A

E

Q

K

V

I

A

P

I

W

P

P

S

L

I

F

P

V

A

H

Y

Y

C

A

A

A

S

S

K

K

G

G

G

G

L

M

M

K

Q

L

L

M

T

T

K

E

V

T

A

L

A

A

L

L

A

E

L

Y

A

A

P

P

H

K

G

G

I

I

R

R

V
|
V

N

N

A

N

V

I

G

G

P

P

G

G

S

A

I

I

D

N

T

T

E
x
P

M

I

M

N

A

A

G

E

V

K

N

F

A

A

N

D

P

P

E

E

A

Q

M

R

N

A

M

D

V

V

L
x
E

S

S

R

M

T

I

P

P

L

M

K

G

R
x
Y

V

I

G

G

T

E

A

P

Q

E

E

E

I

I

A

A

N

A

V

V

V

A

A

A

F

W

L

L

C

A

S

S

D

S

K

E

A

A

S

S

Y

Y

V

V

T

T

G

G

E

I

T

T

I

L

Y

F

V

A

D

D

G

G

G

G

11:35 (vs. 8:32, 36% identical) binding
E96 (≠ A86) mutation E->A,G,K: Heat stable.
D108 (≠ T98) mutation to N: Heat stable.
V112 (= V102) mutation to A: Heat stable.
E133 (≠ A123) mutation to K: Heat stable.
V183 (= V173) mutation to I: Heat stable.
P194 (≠ E184) mutation to Q: Heat stable.
E210 (≠ L200) mutation to K: Heat stable.
Y217 (≠ R207) mutation to H: Heat stable.

Sites not aligning to the query:

252 Q→L: Heat stable.
253 Y→C: Heat stable.
258 A→G: Heat stable.

3d3wA Structure of l-xylulose reductase with bound coenzyme, phosphate and hydroxide. (see paper)
38% identity, 94% coverage: 8:239/247 of query aligns to 11:240/244 of 3d3wA

query
sites
3d3wA

L

L

I

V

T

T

G
|
G

G

A

A
x
G

Q
x
K

G
|
G

I
|
I

G

G

Y

R

A

G

C

T

A

V

E

Q

A

A

L

L

A

H

A

A

E

T

G

G

A

A

R

R

I

V

I

V

-

A

-

V

-
x
S

-
x
R

-
x
T

L

Q

A

A

D

D

I

L

Q

D

D

S

S

L

V

V

Q

R

E

E

A

C

A

P

A

-

R

-

L

-

G

-

G

G

A

I

G

E

Y

P

L

V

C

C

-
x
V

D
|
D

M
x
L

G

G

D

D

A

W

A

E

A

A

V

T

G

-

A

-

L

-

F

-

D

E

R

R

I

A

E

L

A

G

E

S

H

V

G

G

A

P

V

V

T

D

Y

L

L

L

V

V

N

N

N
|
N

A
|
A

G
x
A

I
x
V

A

A

A

L

P

L

G

Q

D

P

F

F

L

L

D

E

Y

V

D

T

L

K

A

E

T

A

F

F

D

D

R
|
R

V

S

L

F

D

E

I
x
V

N

N

L

L

R

R

G

A

V

V

F

I

V

Q

A

V

T

S

Q

Q

R

I

A

V

G

A

R

R

S

G

M

L

V

I

A

A

H

R

G

G

I

V

K

P

G

G

A

A

V

I

V

V

N

N

M
x
V

S
|
S

S
|
S

I

Q

N

C

A

S

Q

Q

V

R

A

A

I

V

P

T

S

N

I
x
H

P

S

A

V

Y
|
Y

C

C

A

S

S

T

K
|
K

G

G

G

A

L

L

M

D

Q

M

L

L

T

T

K

K

V

V

A

M

A

A

L

L

A

E

L

L

A

G

P

P

H

H

G

K

I

I

R

R

V

V

N

N

A

A

V

V

G

N

P
|
P

G
x
T

S
x
V

I
x
V

D

M

T
|
T

E
x
S

M
|
M

M
x
G

A

Q

G

A

V

T

N

W

A

S

N

D

P

P

E

H

A

K

M

A

N

K

M

T

V

M

L

L

S

N

R

R

T

I

P

P

L

L

K

G

R

K

V

F

G

A

T

E

A

V

Q

E

E

H

I

V

A

V

N

N

V

A

V

I

A

L

F

F

L

L

C

L

S

S

D

D

K

R

A

S

S

G

Y

M

V

T

T

T

G

G

E

S

T

T

I

L

Y

P

V

V

D

E

G

G

G

G

active site: G18 (= G15), S136 (= S135), H146 (≠ I145), Y149 (= Y148), K153 (= K152)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G11), G16 (≠ A13), K17 (≠ Q14), G18 (= G15), I19 (= I16), S38 (vs. gap), R39 (vs. gap), T40 (vs. gap), V59 (vs. gap), D60 (= D55), L61 (≠ M56), N83 (= N82), A84 (= A83), A85 (≠ G84), V86 (≠ I85), R102 (= R101), V106 (≠ I105), V134 (≠ M133), S135 (= S134), S136 (= S135), Y149 (= Y148), K153 (= K152), P179 (= P178), T180 (≠ G179), V181 (≠ S180), V182 (≠ I181), T184 (= T183), S185 (≠ E184), M186 (= M185), G187 (≠ M186)

1pr9A Human l-xylulose reductase holoenzyme (see paper)
38% identity, 94% coverage: 8:239/247 of query aligns to 11:240/244 of 1pr9A

query
sites
1pr9A

L

L

I

V

T

T

G
|
G

G

A

A
x
G

Q
x
K

G
|
G

I
|
I

G

G

Y

R

A

G

C

T

A

V

E

Q

A

A

L

L

A

H

A

A

E

T

G

G

A

A

R

R

I

V

I

V

-

A

-

V

-
x
S

-
x
R

-
x
T

L

Q

A

A

D

D

I

L

Q

D

D

S

S

L

V

V

Q

R

E

E

A

C

A

P

A

-

R

-

L

-

G

-

G

G

A

I

G

E

Y

P

L

V

C

C

-
x
V

D
|
D

M
x
L

G

G

D

D

A

W

A

E

A

A

V

T

G

-

A

-

L

-

F

-

D

E

R

R

I

A

E

L

A

G

E

S

H

V

G

G

A

P

V

V

T

D

Y

L

L

L

V

V

N

N

N
|
N

A
|
A

G
x
A

I

V

A

A

A

L

P

L

G

Q

D

P

F

F

L

L

D

E

Y

V

D

T

L

K

A

E

T

A

F

F

D

D

R
|
R

V

S

L

F

D

E

I
x
V

N

N

L

L

R

R

G

A

V

V

F

I

V

Q

A

V

T

S

Q

Q

R

I

A

V

G

A

R

R

S

G

M

L

V

I

A

A

H

R

G

G

I

V

K

P

G

G

A

A

V

I

V

V

N

N

M
x
V

S
|
S

S
|
S

I
x
Q

N
x
C

A

S

Q

Q

V

R

A

A

I

V

P

T

S

N

I
x
H

P

S

A

V

Y
|
Y

C

C

A

S

S

T

K
|
K

G

G

G

A

L

L

M

D

Q

M

L

L

T

T

K

K

V

V

A

M

A

A

L

L

A

E

L

L

A

G

P

P

H

H

G

K

I

I

R

R

V

V

N

N

A

A

V

V

G

N

P
|
P

G
x
T

S
x
V

I
x
V

D

M

T
|
T

E
x
S

M
|
M

M
x
G

A

Q

G

A

V

T

N

W

A

S

N

D

P

P

E

H

A

K

M

A

N

K

M

T

V

M

L

L

S

N

R

R

T

I

P

P

L

L

K

G

R

K

V

F

G

A

T

E

A

V

Q

E

E

H

I

V

A

V

N

N

V

A

V

I

A

L

F

F

L

L

C

L

S

S

D

D

K

R

A

S

S

G

Y

M

V

T

T

T

G

G

E

S

T

T

I

L

Y

P

V

V

D

E

G

G

G

G

active site: G18 (= G15), S136 (= S135), H146 (≠ I145), Y149 (= Y148), K153 (= K152)
binding dihydrogenphosphate ion: S136 (= S135), Q137 (≠ I136), C138 (≠ N137), H146 (≠ I145), Y149 (= Y148), V181 (≠ S180)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G11), G16 (≠ A13), K17 (≠ Q14), G18 (= G15), I19 (= I16), S38 (vs. gap), R39 (vs. gap), T40 (vs. gap), V59 (vs. gap), D60 (= D55), L61 (≠ M56), N83 (= N82), A84 (= A83), A85 (≠ G84), R102 (= R101), V106 (≠ I105), V134 (≠ M133), S135 (= S134), S136 (= S135), Y149 (= Y148), K153 (= K152), P179 (= P178), T180 (≠ G179), V181 (≠ S180), V182 (≠ I181), T184 (= T183), S185 (≠ E184), M186 (= M185), G187 (≠ M186)

Q7Z4W1 L-xylulose reductase; XR; Carbonyl reductase II; Dicarbonyl/L-xylulose reductase; Kidney dicarbonyl reductase; kiDCR; Short chain dehydrogenase/reductase family 20C member 1; Sperm surface protein P34H; EC 1.1.1.10 from Homo sapiens (Human) (see 2 papers)
38% identity, 94% coverage: 8:239/247 of query aligns to 11:240/244 of Q7Z4W1

query
sites
Q7Z4W1

L
|
L

I
x
V

T
|
T

G
|
G

G
x
A

A
x
G

Q
x
K

G
|
G

I
|
I

G
|
G

Y
x
R

A
x
G

C
x
T

A
x
V

E
x
Q

A
|
A

L
|
L

A
x
H

A
|
A

E
x
T

G
|
G

A
|
A

R
|
R

I
x
V

I
x
V

-
x
A

-
x
V

-
x
S

-
x
R

-
x
T

L

Q

A

A

D

D

I

L

Q

D

D

S

S

L

V

V

Q

R

E

E

A

C

A

P

A

-

R

-

L

-

G

-

G

G

A

I

G

E

Y

P

L

V

C

C

-

V

D

D

M

L

G

G

D

D

A

W

A

E

A

A

V

T

G

-

A

-

L

-

F

-

D

E

R

R

I

A

E

L

A

G

E

S

H

V

G

G

A

P

V

V

T

D

Y

L

L

L

V

V

N

N

N

N

A

A

G

A

I

V

A

A

A

L

P

L

G

Q

D

P

F

F

L

L

D

E

Y

V

D

T

L

K

A

E

T

A

F

F

D

D

R

R

V

S

L

F

D

E

I

V

N
|
N

L

L

R

R

G

A

V

V

F

I

V

Q

A

V

T

S

Q

Q

R

I

A

V

G

A

R

R

S

G

M

L

V

I

A

A

H

R

G

G

I

V

K

P

G

G

A

A

V

I

V

V

N

N

M

V

S

S

S
|
S

I

Q

N

C

A

S

Q

Q

V

R

A

A

I

V

P

T

S

N

I

H

P

S

A

V

Y

Y

C

C

A

S

S

T

K
|
K

G

G

G

A

L

L

M

D

Q

M

L

L

T

T

K

K

V

V

A

M

A

A

L

L

A

E

L

L

A

G

P

P

H

H

G

K

I

I

R

R

V

V

N

N

A

A

V

V

G

N

P

P

G

T

S

V

I

V

D

M

T

T

E

S

M

M

M

G

A

Q

G

A

V

T

N

W

A

S

N

D

P

P

E

H

A

K

M

A

N

K

M

T

V

M

L

L

S

N

R

R

T

I

P

P

L

L

K

G

R

K

V

F

G

A

T

E

A

V

Q

E

E

H

I

V

A

V

N

N

V

A

V

I

A

L

F

F

L

L

C

L

S

S

D

D

K

R

A

S

S

G

Y

M

V

T

T

T

G

G

E

S

T

T

I

L

Y

P

V

V

D

E

G

G

G

G

11:40 (vs. 8:32, 40% identical) binding
N107 (= N106) mutation N->L,D: Loss of function. Probably due to defects in formation of the active site and binding of coenzyme.
S136 (= S135) binding
K153 (= K152) binding

Sites not aligning to the query:

1 modified: N-acetylmethionine

2d1yA Crystal structure of tt0321 from thermus thermophilus hb8 (see paper)
42% identity, 94% coverage: 8:239/247 of query aligns to 9:235/240 of 2d1yA

query
sites
2d1yA

L

L

I

V

T

T

G
|
G

G

G

A
|
A

Q
x
R

G
|
G

I
|
I

G

G

Y

R

A

A

C

I

A

A

E

Q

A

A

L

F

A

A

A

R

E

E

G

G

A

A

R

L

I

V

I

A

L

L

A

C

D
|
D

I
x
L

Q
x
R

D

P

S

E

V

G

Q

K

E

E

A

V

A

A

A

E

R

A

L

I

G

G

G

G

A

A

G

F

Y

F

L

Q

C
x
V

D
|
D

M
x
L

G

E

D

D

A

E

A

R

A

E

V

R

G

V

A

R

L

F

F

V

D

E

R

E

I

A

E

A

A

Y

E

A

H

L

G

G

A

R

V

V

T

D

Y

V

L

L

V

V

N

N

N
|
N

A
|
A

G
x
A

I
|
I

A

A

A

A

P

P

G

G

D

S

F

A

L

L

D

T

Y

V

D

R

L

L

A

P

T

E

F

W

D

R

R

R

V

V

L

L

D

E

I
x
V

N

N

L

L

R

T

G

A

V

P

F

M

V

H

A

L

T

S

Q

A

R

L

A

A

G

A

R

R

S

E

M

M

V

R

A

K

H

V

G

G

I

-

K

G

G

G

A

A

V

I

V

V

N

N

M
x
V

S
x
A

S
|
S

I

V

N

Q

A

G

Q

L

V

F

A

A

I

E

P

Q

S

E

I
x
N

P

A

A

A

Y
|
Y

C

N

A

A

S

S

K
|
K

G

G

G

G

L

L

M

V

Q

N

L

L

T

T

K

R

V

S

A

L

A

A

L

L

A

D

L

L

A

A

P

P

H

L

G

R

I

I

R

R

V

V

N

N

A

A

V

V

G

A

P
|
P

G
|
G

S
x
A

I
|
I

D

A

T
|
T

E

E

M
x
A

M
x
V

A

L

G

E

V

A

N

I

A

A

N

R

P

R

E

D

A

W

M

E

N

D

M

L

V

-

L

-

S

-

R

-

T

H

P

A

L

L

K

R

R

R

V

L

G

G

T

K

A

P

Q

E

E

E

I

V

A

A

N

E

V

A

V

V

A

L

F

F

L

L

C

A

S

S

D

E

K

K

A

A

S

S

Y

F

V

I

T

T

G

G

E

A

T

I

I

L

Y

P

V

V

D

D

G

G

G

G

active site: G16 (= G15), S135 (= S135), N145 (≠ I145), Y148 (= Y148), K152 (= K152)
binding nicotinamide-adenine-dinucleotide: G12 (= G11), A14 (= A13), R15 (≠ Q14), G16 (= G15), I17 (= I16), D36 (= D35), L37 (≠ I36), R38 (≠ Q37), V55 (≠ C54), D56 (= D55), L57 (≠ M56), N83 (= N82), A84 (= A83), A85 (≠ G84), I86 (= I85), V106 (≠ I105), V133 (≠ M133), A134 (≠ S134), S135 (= S135), Y148 (= Y148), K152 (= K152), P178 (= P178), G179 (= G179), A180 (≠ S180), I181 (= I181), T183 (= T183), A185 (≠ M185), V186 (≠ M186)

5itvD Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
40% identity, 94% coverage: 8:239/247 of query aligns to 11:223/227 of 5itvD

query
sites
5itvD

L

L

I

I

T

T

G
|
G

G

G

A

A

Q
x
S

G
|
G

I
|
I

G

G

Y

Y

A

A

C

A

A

V

E

Q

A

A

L

F

A

L

A

G

E

Q

G

Q

A

A

R

N

I

V

I

V

L

V

A

A

D
|
D

I
|
I

Q

D

D

E

S

A

V

Q

Q

G

E

E

A

A

A

M

A

V

R

R

L

K

G

E

G

N

A

N

G

D

Y

R

L

L

-

H

-

F

-

V

-

Q

C
x
T

D
|
D

M
x
I

G

T

D

D

A

E

A

A

A

A

V

C

G

Q

A

H

L

A

F

V

D

E

R

S

I

A

E

V

A

H

E

T

H

F

G

G

A

G

V

L

T

D

Y

V

L

L

V

I

N

N

N
|
N

A
|
A

G
|
G

I
|
I

A

E

A

I

P

V

G

A

D

P

F

I

L

H

D

E

Y

M

D

E

L

L

A

S

T

D

F

W

D

N

R

K

V

V

L

L

D

Q

I
x
V

N

N

L

L

R

T

G

G

V

M

F

F

V

L

A

M

T

S

Q

K

R

H

A

A

G

L

R

K

S

H

M

M

V

L

A

A

H

A

G

G

I

-

K

K

G

G

A

N

V

I

V

I

N

N

M
x
T

S
x
C

S
|
S

I

V

N

G

A

G

Q

L

V

V

A

A

I

W

P

P

S

D

I

I

P

P

A

A

Y
|
Y

C

N

A

A

S

S

K
|
K

G

G

G

G

L

V

M

L

Q

Q

L

L

T

T

K

K

V

S

A

M

A

A

L

V

A

D

L

Y

A

A

P

K

H

H

G

Q

I

I

R

R

V

V

N

N

A

C

V

V

G

C

P
|
P

G
|
G

S
x
I

I
|
I

D

D

T
|
T

E

-

M

-

M

-

A

-

G

-

V

-

N

-

A

-

N

-

P

-

E

-

A

-

M

-

N

-

M

-

V

-

L

-

S

-

R

-

T

-

P

-

L

-

K

L

R

R

V

L

G

G

T

K

A

P

Q

E

E

E

I

I

A

A

N

N

V

V

V

M

A

L

F

F

L

L

C

A

S

S

D

D

K

L

A

S

S

S

Y

Y

V

M

T

T

G

G

E

S

T

A

I

I

Y

T

V

A

D

D

G

G

G

G

active site: G18 (= G15), S141 (= S135), Y154 (= Y148), K158 (= K152)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G11), S17 (≠ Q14), G18 (= G15), I19 (= I16), D38 (= D35), I39 (= I36), T61 (≠ C54), D62 (= D55), I63 (≠ M56), N89 (= N82), A90 (= A83), G91 (= G84), I92 (= I85), V112 (≠ I105), T139 (≠ M133), C140 (≠ S134), S141 (= S135), Y154 (= Y148), K158 (= K152), P184 (= P178), G185 (= G179), I186 (≠ S180), I187 (= I181), T189 (= T183)

4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
41% identity, 95% coverage: 6:239/247 of query aligns to 12:249/258 of 4wecA

query
sites
4wecA

L

V

A

A

L

V

I

I

T

T

G
|
G

G

G

A
|
A

Q
x
S

G
|
G

I
|
I

G

G

Y

L

A

A

C

T

A

G

E

R

A

R

L

L

A

R

A

A

E

E

G

G

A

A

R

T

I

V

I

V

L

V

A

G

D
|
D

I
|
I

Q
x
D

D

P

S

T

V

T

Q

G

E

K

A

A

A

A

A

A

-

D

R

E

L

L

G

E

G

G

A

L

G

F

Y

V

L

P

C
x
V

D
|
D

M
x
V

G

S

D

E

A

Q

A

E

A

A

V

V

G

D

A

N

L

L

F

F

D

D

R

T

I

A

E

A

A

S

E

T

H

F

G

G

A

R

V

V

T

D

Y

I

L

A

V

F

N

N

N
|
N

A
|
A

G
|
G

I
|
I

A

S

A

P

P

P

G

E

D

D

F

D

L

L

-

I

-

E

D

N

Y

T

D

D

L

L

A

P

T

A

F

W

D

Q

R

R

V

V

L

Q

D

D

I

I

N

N

L

L

R

K

G

S

V

V

F

Y

V

L

A

S

T

C

Q

R

R

A

A

A

G

L

R

R

S

H

M

M

V

V

A

P

H

A

G

G

I

-

K

K

G

G

A

S

V

I

V

I

N

N

M
x
T

S
x
A

S
|
S

I

F

N

V

A

A

Q

V

V

M

-

G

A

S

I

A

P

T

S

S

I
x
Q

P

I

A

S

Y
|
Y

C

T

A

A

S

S

K
|
K

G

G

G

G

L

V

M

L

Q

A

L

M

T

S

K

R

V

E

A

L

A

G

L

V

A

Q

L

Y

A

A

P

R

H

Q

G

G

I

I

R

R

V

V

N

N

A

A

V

L

G

C

P
|
P

G
|
G

S
x
P

I
x
V

D

N

T

T

E

P

M

L

M

L

A

Q

G

E

V

L

N

F

A

A

-

K

N

D

P

P

E

E

A

R

M

A

N

A

M

R

V

R

L

L

S

V

R

H

T

I

P

P

L

L

K

G

R

R

V

F

G

A

T

E

A

P

Q

E

E

E

I

L

A

A

N

A

V

A

V

V

A

A

F

F

L

L

C

A

S

S

D

D

K

D

A

A

S

S

Y

F

V

I

T

T

G

G

E

S

T

T

I

F

Y

L

V

V

D

D

G

G

G

G

active site: G21 (= G15), S143 (= S135), Q154 (≠ I145), Y157 (= Y148), K161 (= K152)
binding nicotinamide-adenine-dinucleotide: G17 (= G11), A19 (= A13), S20 (≠ Q14), G21 (= G15), I22 (= I16), D41 (= D35), I42 (= I36), D43 (≠ Q37), V61 (≠ C54), D62 (= D55), V63 (≠ M56), N89 (= N82), A90 (= A83), G91 (= G84), I92 (= I85), T141 (≠ M133), A142 (≠ S134), S143 (= S135), Y157 (= Y148), K161 (= K152), P187 (= P178), G188 (= G179), P189 (≠ S180), V190 (≠ I181)

1cydA Carbonyl reductase complexed with NADPH and 2-propanol (see paper)
36% identity, 95% coverage: 7:241/247 of query aligns to 8:240/242 of 1cydA

query
sites
1cydA

A

A

L

L

I

V

T

T

G
|
G

G

A

A
x
G

Q
x
K

G
|
G

I
|
I

G

G

Y

R

A

D

C

T

A

V

E

K

A

A

L

L

A

H

A

A

E

S

G

G

A

A

R

K

I

V

I

V

L

A

A

V

D
x
T

I
x
R

Q
x
T

D

N

S

S

-

D

V

L

Q

V

E

S

A

L

A

A

A

K

R

E

L

C

G

P

G

G

A

I

G

E

Y

P

L

V

C

C

-
x
V

D
|
D

M
x
L

G

G

D

D

A

W

A

D

A

A

V

T

G

E

A

K

L

A

F

L

D

G

R

G

I

I

E

-

A

-

E

-

H

-

G

G

A

P

V

V

T

D

Y

L

L

L

V

V

N

N

N
|
N

A
|
A

G
x
A

I

L

A

V

A

I

P

M

G

Q

D

P

F

F

L

L

D

E

Y

V

D

T

L

K

A

E

T

A

F

F

D

D

R

R

V

S

L

F

D

S

I
x
V

N

N

L

L

R

R

G

S

V

V

F

F

V

Q

A

V

T

S

Q

Q

R

M

A

V

G

A

R

R

S

D

M

M

V

I

A

N

H

R

G

G

I

V

K

P

G

G

A

S

V

I

V

V

N

N

M
x
V

S
|
S

S
|
S

I

M

N

V

A

A

Q

H

V

V

A

T

I

F

P

P

S

N

I
x
L

P

I

A

T

Y
|
Y

C

S

A

S

S

T

K
|
K

G

G

G

A

L

M

M

T

Q

M

L

L

T

T

K

K

V

A

A

M

A

A

L

M

A

E

L

L

A

G

P

P

H

H

G

K

I

I

R

R

V

V

N

N

A

S

V

V

G

N

P
|
P

G
x
T

S
x
V

I
x
V

D

L

T
|
T

E
x
D

M
|
M

M
x
G

A

K

G

K

V
|
V

N

S

A

A

N

D

P

P

E

E

A

F

M

A

N

R

M

K

V

L

L

K

S

E

R

R

T

H

P

P

L

L

K

R

R

K

V

F

G

A

T

E

A

V

Q

E

E

D

I

V

A

V

N

N

V

S

V

I

A

L

F

F

L

L

C

L

S

S

D

D

K

R

A

S

S

A

Y

S

V

T

T

S

G

G

E

G

T

G

I

I

Y

L

V

V

D

D

G

A

G

G

R

Y

L

L

active site: G16 (= G15), S134 (= S135), L144 (≠ I145), Y147 (= Y148), K151 (= K152)
binding isopropyl alcohol: S134 (= S135), Y147 (= Y148), V188 (= V189)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G11), G14 (≠ A13), K15 (≠ Q14), G16 (= G15), I17 (= I16), T36 (≠ D35), R37 (≠ I36), T38 (≠ Q37), V57 (vs. gap), D58 (= D55), L59 (≠ M56), N81 (= N82), A82 (= A83), A83 (≠ G84), V104 (≠ I105), V132 (≠ M133), S133 (= S134), S134 (= S135), Y147 (= Y148), K151 (= K152), P177 (= P178), T178 (≠ G179), V179 (≠ S180), V180 (≠ I181), T182 (= T183), D183 (≠ E184), M184 (= M185), G185 (≠ M186)

P08074 Carbonyl reductase [NADPH] 2; Adipocyte protein P27; AP27; Lung carbonyl reductase; LCR; NADPH-dependent carbonyl reductase 2; EC 1.1.1.184 from Mus musculus (Mouse) (see 2 papers)
36% identity, 95% coverage: 7:241/247 of query aligns to 10:242/244 of P08074

query
sites
P08074

A

A

L
|
L

I
x
V

T
|
T

G
|
G

G
x
A

A
x
G

Q
x
K

G
|
G

I
|
I

G
|
G

Y
x
R

A
x
D

C
x
T

A
x
V

E
x
K

A
|
A

L
|
L

A
x
H

A
|
A

E
x
S

G
|
G

A
|
A

R
x
K

I
x
V

I
x
V

L
x
A

A
x
V

D
x
T

I
x
R

Q

T

D

N

S

S

-

D

V

L

Q

V

E

S

A

L

A

A

A

K

R

E

L

C

G

P

G

G

A

I

G

E

Y

P

L

V

C

C

-

V

D

D

M

L

G

G

D

D

A

W

A

D

A

A

V

T

G

E

A

K

L

A

F

L

D

G

R

G

I

I

E

-

A

-

E

-

H

-

G

G

A

P

V

V

T

D

Y

L

L

L

V

V

N

N

N

N

A

A

G

A

I

L

A

V

A

I

P

M

G

Q

D

P

F

F

L

L

D

E

Y

V

D

T

L

K

A

E

T

A

F

F

D

D

R

R

V

S

L

F

D

S

I

V

N

N

L

L

R

R

G

S

V

V

F

F

V

Q

A

V

T

S

Q

Q

R

M

A

V

G

A

R

R

S

D

M

M

V

I

A

N

H

R

G

G

I

V

K

P

G

G

A

S

V

I

V

V

N

N

M

V

S

S

S

S

I

M

N

V

A

A

Q

H

V

V

A

T

I

F

P

P

S

N

I

L

P

I

A

T

Y

Y

C

S

A

S

S

T

K

K

G

G

G

A

L

M

M

T

Q

M

L

L

T

T

K

K

V

A

A

M

A

A

L

M

A

E

L

L

A

G

P

P

H

H

G

K

I

I

R

R

V

V

N

N

A

S

V

V

G

N

P

P

G

T

S

V

I

V

D

L

T

T

E

D

M

M

M

G

A

K

G

K

V

V

N

S

A

A

N

D

P

P

E

E

A

F

M

A

N

R

M

K

V

L

L

K

S

E

R

R

T

H

P

P

L

L

K

R

R

K

V

F

G

A

T

E

A

V

Q

E

E

D

I

V

A

V

N

N

V

S

V

I

A

L

F

F

L

L

C

L

S

S

D

D

K

R

A

S

S

A

Y

S

V

T

T

S

G

G

E

G

T

G

I

I

Y

L

V

V

D

D

G

A

G

G

R

Y

L

L

11:39 (vs. 8:36, 38% identical) binding
T38 (≠ D35) mutation to R: Converts the coenzyme specificity from NADP to NAD.

1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
34% identity, 95% coverage: 6:239/247 of query aligns to 8:248/252 of 1vl8B

query
sites
1vl8B

L

V

A

A

L

L

I

V

T

T

G
|
G

G

G

A
x
S

Q
x
R

G
|
G

I
x
L

G

G

Y

F

A

G

C

I

A

A

E

Q

A

G

L

L

A

A

A

E

E

A

G

G

A

C

R

S

I

V

I

V

L

V

A

A

D
x
S

I
x
R

Q
x
N

-

L

D

E

S

E

V

A

Q

S

E

E

A

A

A

A

A

Q

R

K

L

L

G

T

G

E

A

K

-

Y

-

G

-

V

-

E

-

T

-

M

G

A

Y

F

L

R

C
|
C

D
|
D

M
x
V

G

S

D

N

A

Y

A

E

A

E

V

V

G

K

A

K

L

L

F

L

D

E

R

A

I

V

E

K

A

E

E

K

H

F

G

G

A

K

V

L

T

D

Y

T

L

V

V

V

N

N

N
x
A

A
|
A

G
|
G

I
|
I

A

N

A

R

P

R

G

H

D

P

F

A

L

E

D

E

Y

F

D

P

L

L

A

D

T

E

F

F

D

R

R

Q

V

V

L

I

D

E

I
x
V

N

N

L

L

R

F

G

G

V

T

F

Y

V

Y

A

V

T

C

Q

R

R

E

A

A

G

F

R

-

S

S

M

L

V

L

A

R

H

E

G

S

I

D

K

N

G

P

A

S

V

I

V

I

N

N

M
x
I

S
x
G

S
|
S

I

L

N

T

A

V

Q

E

-

E

V

V

A

T

I

M

P

P

S

N

I
|
I

P

S

A

A

Y
|
Y

C

A

A

A

S

S

K
|
K

G

G

G

G

L

V

M

A

Q

S

L

L

T

T

K

K

V

A

A

L

A

A

L

K

A

E

L

W

A

G

P

R

H

Y

G

G

I

I

R

R

V

V

N

N

A

V

V

I

G

A

P
|
P

G
|
G

S
x
W

I
x
Y

D

R

T
|
T

E
x
K

M
|
M

M
x
T

A

E

G

A

V

V

N

F

A

S

N

D

P

P

E

E

A

K

M

L

N

D

M

Y

V

M

L

L

S

K

R

R

T

I

P

P

L

L

K

G

R

R

V

T

G

G

T

V

A

P

Q

E

E

D

I

L

A

K

N

G

V

V

V

A

A

V

F

F

L

L

C

A

S

S

D

E

K

E

A

A

S

K

Y

Y

V

V

T

T

G

G

E

Q

T

I

I

I

Y

F

V

V

D

D

G

G

G

G

active site: G17 (= G15), S143 (= S135), I154 (= I145), Y157 (= Y148), K161 (= K152)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G11), S15 (≠ A13), R16 (≠ Q14), G17 (= G15), L18 (≠ I16), S37 (≠ D35), R38 (≠ I36), N39 (≠ Q37), C63 (= C54), D64 (= D55), V65 (≠ M56), A91 (≠ N82), A92 (= A83), G93 (= G84), I94 (= I85), V114 (≠ I105), I141 (≠ M133), G142 (≠ S134), S143 (= S135), Y157 (= Y148), K161 (= K152), P187 (= P178), G188 (= G179), W189 (≠ S180), Y190 (≠ I181), T192 (= T183), K193 (≠ E184), M194 (= M185), T195 (≠ M186)

7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh
40% identity, 96% coverage: 4:239/247 of query aligns to 8:244/247 of 7tzpG

query
sites
7tzpG

T

S

P

K

L

T

A

A

L

I

I

V

T

T

G
|
G

G

A

A
|
A

Q
x
R

G
|
G

I
|
I

G

G

Y

F

A

G

C

I

A

A

E

Q

A

V

L

L

A

A

A

R

E

E

G

G

A

A

R

R

I

V

I

I

L

I

A

A

D
|
D

I

-

Q
x
R

D

D

S

A

V

H

Q

G

E

E

A

A

A

A

A

A

-

A

-

S

-

L

R

R

L

E

G

S

G

G

A

A

G

Q

Y

A

L

L

-

F

-

I

-

S

C
|
C

D
x
N

M
x
I

G

A

D

E

A

K

A

T

A

Q

V

V

G

E

A

A

L

L

F

F

D

S

R

Q

I

A

E

E

A

E

E

A

H

F

G

G

A

P

V

V

T

D

Y

I

L

L

V

V

N

N

N
|
N

A
|
A

G
|
G

I
|
I

A

N

A

R

P

D

G

A

D

M

F

L

L

H

D

K

Y

L

D

T

L

E

A

A

T

D

F

W

D

D

R

T

V

V

L

I

D

D

I
x
V

N

N

L

L

R

K

G

G

V

T

F

F

V

L

A

C

T

M

Q

Q

R

Q

A

A

G

A

R

I

S

R

M

M

V

R

A

E

H

R

G

G

I

-

K

A

G

G

A

R

V

I

V

I

N

N

M
x
I

S

A

S
|
S

I

A

N

S

A

W

Q

L

V

G

A

N

I

V

P

G

S

Q

I

T

P

N

A

-

Y
|
Y

C

S

A

A

S

S

K
|
K

G

A

G

G

L

V

M

V

Q

G

L

M

T

T

K

K

V

T

A

A

A

C

L

R

A

E

L

L

A

A

P

K

H

K

G

G

I

V

R

T

V

V

N

N

A

A

V

I

G

C

P
|
P

G
|
G

S
x
F

I
|
I

D

D

T
|
T

E

D

M
|
M

M
x
T

A

R

G

G

V

V

N

P

A

E

N

N

P

-

E

-

A

V

M

W

N

Q

M

I

V

M

L

V

S

S

R

K

T

I

P

P

L

A

K

G

R

Y

V

A

G

G

T

E

A

A

Q

K

E

D

I

V

A

G

N

E

V

C

V

V

A

A

F

F

L

L

C

A

S

S

D

D

K

G

A

A

S

R

Y

Y

V

I

T

N

G

G

E

E

T

V

I

I

Y

N

V

V

D

G

G

G

G

G

binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G11), A17 (= A13), R18 (≠ Q14), G19 (= G15), I20 (= I16), D39 (= D35), R40 (≠ Q37), C63 (= C54), N64 (≠ D55), I65 (≠ M56), N91 (= N82), A92 (= A83), G93 (= G84), I94 (= I85), V114 (≠ I105), I141 (≠ M133), S143 (= S135), Y155 (= Y148), K159 (= K152), P185 (= P178), G186 (= G179), F187 (≠ S180), I188 (= I181), T190 (= T183), M192 (= M185), T193 (≠ M186)

3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
38% identity, 97% coverage: 1:239/247 of query aligns to 1:243/246 of 3osuA

query
sites
3osuA

M

M

S

K

Q

M

T

T

P

K

L

S

A

A

L

L

I

V

T

T

G

G

G

A

A

S

Q

R

G
|
G

I

I

G

G

Y

R

A

S

C

I

A

A

E

L

A

Q

L

L

A

A

A

E

E

E

G

G

A

Y

R

N

I

V

I

A

L

V

-

N

-

Y

-

A

-

G

-

S

-

K

A

E

D

K

I

A

Q

E

D

A

S

V

V

V

Q

E

E

E

A

I

A

K

A

A

R

K

-

G

L

V

G

D

G

S

A

F

G

A

Y

I

L

Q

C

A

D

N

M

V

G

A

D

D

A

A

A

D

A

E

V

V

G

K

A

A

L

M

F

I

D

K

R

E

I

V

E

V

A

S

E

Q

H

F

G

G

A

S

V

L

T

D

Y

V

L

L

V

V

N

N

N
|
N

A

A

G

G

I

I

A

T

A

R

P

D

G

N

D

L

F

L

L

M

D

R

Y

M

D

K

L

E

A

Q

T

E

F

W

D

D

R

D

V

V

L

I

D

D

I

T

N

N

L

L

R

K

G

G

V

V

F

F

V

N

A

C

T

I

Q

Q

R

K

A

A

G

T

R

P

S

Q

M

M

V

L

A

R

H

Q

G

R

I

-

K

S

G

G

A

A

V

I

V

I

N

N

M

L

S

S

S
|
S

I

V

N

V

A

G

Q

A

V

V

A

G

I

N

P

P

S

G

I
x
Q

P

A

A

N

Y
|
Y

C

V

A

A

S

T

K
|
K

G

A

G

G

L

V

M

I

Q

G

L

L

T

T

K

K

V

S

A

A

A

A

L

R

A

E

L

L

A

A

P

S

H

R

G

G

I

I

R

T

V

V

N

N

A

A

V

V

G

A

P

P

G

G

S

F

I

I

D

V

T

S

E

D

M

M

M

T

A

D

G

A

V

L

N

S

A

-

N

-

P

D

E

E

A

L

M

K

N

E

M

Q

V

M

L

L

S

T

R

Q

T

I

P

P

L

L

K

A

R

R

V

F

G

G

T

Q

A

D

Q

T

E

D

I

I

A

A

N

N

V

T

V

V

A

A

F

F

L

L

C

A

S

S

D

D

K

K

A

A

S

K

Y

Y

V

I

T

T

G

G

E

Q

T

T

I

I

Y

H

V

V

D

N

G

G

G

G

active site: G15 (= G15), S141 (= S135), Q151 (≠ I145), Y154 (= Y148), K158 (= K152)
binding magnesium ion: N89 (= N82), K158 (= K152)

3ay6B Crystal structure of bacillus megaterium glucose dehydrogenase 4 a258f mutant in complex with nadh and d-glucose (see paper)
36% identity, 95% coverage: 6:239/247 of query aligns to 15:255/267 of 3ay6B

query
sites
3ay6B

L

V

A

V

L

V

I

I

T

T

G
|
G

G

G

A

S

Q
x
T

G
|
G

I
x
L

G

G

Y

R

A

A

C

M

A

A

E

V

A

R

L

F

A

G

A

Q

E

E

G

E

A

A

R

K

I

V

I

V

L

I

A

N

-

Y

-
x
Y

-

N

-

N

-

E

-

E

-

E

-

A

-

L

D

D

I

A

Q

K

D

K

S

E

V

V

Q

E

E

E

A

A

A

-

A

-

R

-

L

-

G

G

G

G

A

Q

G

A

Y

I

L

I

C

V

-

Q

-
x
G

D
|
D

M
x
V

G

T

D

K

A

E

A

E

A

D

V

V

G

V

A

N

L

L

F

V

D

Q

R

T

I

A

E

I

A

K

E

E

H

F

G

G

A

T

V

L

T

D

Y

V

L

M

V

I

N

N

N
|
N

A
|
A

G
|
G

I
x
V

A
x
E

A

N

P

P

G

V

D

P

F

S

L

H

D

E

Y

L

D

S

L

L

A

D

T

N

F

W

D

N

R

K

V

V

L

I

D

D

I
x
T

N

N

L

L

R

T

G

G

V

A

F

F

V

L

A

G

T

S

Q

R

R

E

A

A

G

I

R

K

S

Y

M

F

V

V

A

E

H

N

G

D

I

I

K

K

G

G

A

N

V

V

V

I

N

N

M
|
M

S
|
S

S
|
S

I
x
V

N
x
H

A

E

Q

M

V

I

A

P

I
x
W

P

P

S

L

I

F

P

V

A

H

Y
|
Y

C

A

A

A

S

S

K
|
K

G

G

G

G

L

M

M

K

Q

L

L

M

T

T

K

E

V

T

A

L

A

A

L

L

A

E

L

Y

A

A

P

P

H

K

G

G

I

I

R

R

V

V

N

N

A

N

V

I

G

G

P
|
P

G
|
G

S
x
A

I
x
M

D

N

T
|
T

E
x
P

M
x
I

M
x
N

A

A

G

E

V
x
K

N

F

A

A

N

D

P

P

E

V

A

Q

M

R

N

A

M

D

V

V

L

E

S

S

R

M

T

I

P

P

L

M

K

G

R

Y

V

I

G

G

T

K

A

P

Q

E

E

E

I

V

A

A

N

A

V

V

V

A

A

A

F

F

L

L

C

A

S

S

D

S

K

Q

A

A

S

S

Y

Y

V

V

T

T

G

G

E

I

T

T

I

L

Y

F

V

A

D

D

G

G

G

G

active site: G24 (= G15), S151 (= S135), Y164 (= Y148), K168 (= K152)
binding beta-D-glucopyranose: E102 (≠ A86), S151 (= S135), V152 (≠ I136), H153 (≠ N137), W158 (≠ I142), Y164 (= Y148), G195 (= G179), A196 (≠ S180), I201 (≠ M185), N202 (≠ M186), K205 (≠ V189)
binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G11), T23 (≠ Q14), G24 (= G15), L25 (≠ I16), Y45 (vs. gap), G70 (vs. gap), D71 (= D55), V72 (≠ M56), N98 (= N82), A99 (= A83), G100 (= G84), V101 (≠ I85), T121 (≠ I105), M149 (= M133), S150 (= S134), S151 (= S135), Y164 (= Y148), K168 (= K152), P194 (= P178), G195 (= G179), A196 (≠ S180), M197 (≠ I181), T199 (= T183), P200 (≠ E184), I201 (≠ M185), N202 (≠ M186)

Sites not aligning to the query:

binding beta-D-glucopyranose: 256

Q6WVP7 NADP-dependent (R)-specific alcohol dehydrogenase; (R)-specific ADH; Ketoreductase; KRED; EC 1.1.1.- from Lentilactobacillus kefiri (Lactobacillus kefiri) (see paper)
37% identity, 95% coverage: 6:239/247 of query aligns to 9:248/252 of Q6WVP7

query
sites
Q6WVP7

L

V

A

A

L

I

I

V

T

T

G

G

G

G

A
x
T

Q
x
L

G
|
G

I
|
I

G

G

Y

L

A

A

C

I

A

A

E

D

A

K

L

F

A

V

A

E

E

E

G

G

A

A

R

K

I

V

I

V

L

I

A

T

D

G

I
x
R

Q
x
H

D

A

S

D

V

V

Q

G

E

E

A

K

A

A

A

A

R

K

-

S

L

I

G

G

G

G

A

T

G

D

Y

V

L

I

-

R

-

F

-

V

-

Q

C

H

D
|
D

M
x
A

G

S

D

D

A

E

A

A

A

G

V

W

G

T

A

K

L

L

F

F

D

D

R

T

I

T

E

E

A

E

E

A

H

F

G

G

A

P

V

V

T

T

Y

T

L

V

V

V

N

N

N
|
N

A

A

G

G

I

I

A

A

A

V

P

S

G

K

D

S

F

V

L

E

D

D

Y

T

D

T

L

T

A

E

T

E

F

W

D

R

R

K

V

L

L

L

D

S

I

V

N

N

L

L

R

D

G

G

V

V

F

F

V

F

A

G

T

T

Q

R

R

L

A

G

G

I

R

Q

S

R

M

M

V

K

A

N

H

K

G

G

I

L

K

G

G

A

A

S

V

I

V

I

N

N

M

M

S

S

S

S

I

I

N

E

A

G

Q

F

V

V

A

G

I

D

P

P

S

T

I

L

P

G

A

A

Y
|
Y

C

N

A

A

S

S

K
|
K

G

G

G

A

L

V

M

R

Q

I

L

M

T

S

K

K

V

S

A

A

A

A

L

L

-

D

-

C

A

A

L

L

A

K

P

D

H

Y

G

D

I

V

R

R

V

V

N

N

A

T

V

V

G

H

P

P

G

G

S

Y

I
|
I

D
x
K

T
|
T

E
x
P

M
x
L

M

V

A

D

G

D

V

L

N

E

A

G

N

A

P

E

E

E

A

M

M

M

N

S

M

Q

V

-

L

R

S

T

R

K

T

T

P

P

L

M

K

G

R

H

V

I

G

G

T

E

A

P

Q

N

E

D

I

I

A

A

N

W

V

I

V

C

A

V

F

Y

L

L

C

A

S

S

D

D

K

E

A

S

S

K

Y

F

V

A

T

T

G

G

E

A

T

E

I

F

Y

V

V

V

D

D

G