SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3607863 FitnessBrowser__Dino:3607863 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

Q81RQ4 3-dehydroshikimate dehydratase; 3-DHS dehydratase; DHSase; Petrobactin biosynthesis protein AsbF; EC 4.2.1.118 from Bacillus anthracis (see paper)
30% identity, 47% coverage: 117:255/298 of query aligns to 105:241/280 of Q81RQ4

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Q81RQ4

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E142 (≠ H156) binding ; binding
H144 (= H158) mutation to A: No activity.
D172 (= D186) binding ; binding
H175 (= H189) binding ; mutation to A: Only trace activity remaining.
H198 (= H212) binding
K200 (= K214) mutation K->A,E: No activity.; mutation to R: Less than 5% activity remaining.
Y217 (≠ F231) binding ; mutation to A: Binds substrate with 10-fold lower affinity; when associated with A-70.

Sites not aligning to the query:

70 binding ; Y→A: Binds substrate with 10-fold lower affinity; when associated with A-217.
102 binding ; R→A: No activity.
253 binding ; binding

3dx5A Crystal structure of the probable 3-dhs dehydratase asbf involved in the petrobactin synthesis from bacillus anthracis (see paper)
27% identity, 57% coverage: 85:255/298 of query aligns to 79:241/274 of 3dx5A

query
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3dx5A

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binding 3,4-dihydroxybenzoic acid: R102 (≠ P115), E142 (≠ H156), H144 (= H158), H175 (= H189), K200 (= K214), F211 (≠ I225), Y217 (≠ F231)
binding manganese (ii) ion: E142 (≠ H156), D172 (= D186), H198 (= H212)

Sites not aligning to the query:

Query Sequence

>3607863 FitnessBrowser__Dino:3607863
MTIRIGNAPCSWGVEFAGDPRNPDWRQVLRETAAAGYTGIELGPIGFMPEDPPQVADALA
EHELELIGGVVFRPFHDRAAWEEVLDASVRTCKALVAHGAQHLVLIDSISPRRAPTAGRA
GAAEQMDAVEWAAFRDRIAHVARMGADEYGLTVGIHAHAAGFMDFEPELERLLDEVDDSI
LKICFDTGHHSYAGFDPVAFMQRHLPRISYMHFKDIDPAVKANVIARGTGFYDACGQGIF
CNLGEGDVNFPRVRQLLIDHGFDGWCTVEQDCDPTLPDTDPFGDAMTNRAYLRAIGFE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory