SitesBLAST

L334 (≠ F246) mutation to W: Ability to transport lactose in a saturable manner.
L372 (≠ V286) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ E290) mutation N->K,H: No growth on maltose.
G380 (≠ Y294) mutation G->C,S: No growth on maltose.
E401 (= E315) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A317) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G321) mutation G->A,P: No effect.
P420 (= P334) mutation to A: No effect.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
30% identity, 52% coverage: 179:396/416 of query aligns to 247:480/490 of 4ki0F

query
sites
4ki0F

E

K

N

N

F

F

A

T

R

R

I

V

F

F

D

-

A

T

D

D

E

E

-

G

-

I

-

Q

-

K

-

P

F

F

W

L

G

A

V

I

L

F

G

V

V

W

T

T

M

V

F

V

Y

F

T

S

V

L

F

I

G

T

T

V

L

F

G

L

A

T

L

V

L

A

F

V

G

G

L

M

F

V

A

L

A

A

L

C

L

L

L

V

N

Q

-

W

K

E

S

A

F

L

R

R

G

G

Q

K

G

A

I

V

L

Y

R

R

G

V

L

L

Y

L

L

I

F

L

P

P

Y
|
Y

V

A

A

V

P

P

V

S

I

F

A

I

V

S

A

I

F

L

T

I

W

F

V

K

T

G

L

L

F

F

D

N

P

Q

F

S

S

F

G

G

S

E

A

I

N

N

A

M

L

M

L

L

I

S

Q

A

M

L

G

F

V

G

T

V

N

K

E

P

A

A

I

-

N

-

F

W

F

F

G

S

Q

D

R

P

P

T

L

T

A

A

L

R

I

T

M

M

V

L

T

I

V

I

F

V

E
x
N

I

T

W

W

R

L

Y
x
G

F

Y

P

P

L
x
Y

S

M

F

M

L

I

F

L

I

C

L

M

A

G

R

L

M

L

Q

K

S

A

I

I

D

P

T

D

D

D

M

L

Y

Y

E

E

A

A

A

S

D

A

M

M

D

D

G

G

A

A

S

G

P

P

F

F

Q

Q

K

N

F

F

W

F

Y

K

L

I

S

T

L

L

P

P

M

L

L

L

V

I

G

K

I

P

L

L

S

T

V

P

L

L

F

M

L

I

L

A

R
x
S

F

F

I

A

W

F

T
x
N

F

F

N

N

K

N

F

F

D

V

D

L

I

I

F

Q

L

L

T

T

G

N

G

G

N

G

-

P

-

D

-

R

-

L

-

G

-

T

-

P

A

A

G

G

T

Y

R

T

T

D

L

L

T

L

V

V

N

N

-

Y

V

T

Y

Y

E

R

Q

I

A

A

F

F

-

E

-

G

A

G

V

G

S

Q

N

D

I

F

G

G

A

L

G

A

A

A

V

I

A

A

V

T

V

L

I

I

F

F

binding alpha-D-glucopyranose: Y310 (= Y237), N361 (≠ E290), G365 (≠ Y294), Y368 (≠ L297), S418 (≠ R347), N422 (≠ T351)

P94529 Arabinooligosaccharides transport system permease protein AraP from Bacillus subtilis (strain 168) (see paper)
26% identity, 51% coverage: 178:391/416 of query aligns to 72:287/313 of P94529

query
sites
P94529

L

L

E

S

N

N

F

Y

A

T

R

A

I

L

F

-

D

N

A

N

D

P

E

T

F

F

W

Y

G

T

V

A

L

L

G

W

V

N

T

T

M

L

F

E

Y

Y

T

T

V

F

F

W

G

T

T

L

L

I

G

V

A

L

L

I

L

P

F

V

G

P

L

L

F

L

A

L

A

A

L

I

L

F

L

L

N

N

-

S

K

K

S

L

F

V

R

K

G

F

Q

R

G

N

I

I

L

F

R

K

G

S

L

A

Y

L

L

F

F

I

P

P

Y

A

V

L

A

T

P

S

V

T

I

I

A

V

V

A

A

G

F

I

T

I

W

F

V

R

T

L

L

I

F

F

D

G

P

E

F

M

S

E

G

T

S

S

-

L

A

A

N

N

A

S

L

I

L

L

I

L

Q

K

M

L

G

G

V

F

T

S

N

P

E

Q

A

-

I

-

N

N

F

W

F

M

G

N

Q

N

R

E

P

H

L

T

A

G

L

M

I

F

M

L

V

M

T

V

V

L

F

L

E

A

I

S

W

W

R

R

Y

W

F

M

P

G

L

I

S

N

F

I

L

L

F

Y

I

F

L

L

A

A

R

G

M

L

Q

Q

S

N

I

V

D

P

T

K

D
x
E

M

L

Y

Y

E
|
E

A

A

D

D

M

I

D
|
D

G

G

A

A

S

N

P

T

F

M

Q

K

K

K

F

F

W

L

Y

H

L

I

S

T

L

L

P

P

M

F

L

L

V

K

G

P

I

V

L

T

S

V

V

Y

L

V

F

L

L

T

L

I

R

S

F

I

I

I

W

G

T

G

F

F

N

R

K

M

F

F

D

E

I

S

F

Y

L

V

L

L

-

W

-

Q

-

N

T

S

G

P

G

G

N

N

A

I

G

G

T

L

R

-

T

T

L

L

T

V

V

G

N

Y

V

L

Y

Y

E

Q

Q

Q

A

G

F

L

A

A

V

Y

S

N

N

E

I

M

G

G

A

Y

G

G

A

A

V

I

E205 (≠ D312) mutation to A: Has no effect on the growth rate.
E208 (= E315) mutation to A: Small negative impact in the uptake of arabinotriose. Shows an increase of about 10% in the doubling time.
D213 (= D320) mutation to A: Severely impairs the uptake of arabinotriose. Shows a 3.6-fold increase of the doubling time.

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
49% identity, 10% coverage: 307:349/416 of query aligns to 182:224/296 of P68183

query
sites
P68183

Q

E

S

T

I

I

D

D

T

S

D

S

M

L

Y

E

E
|
E

A

A

A
|
A

D

A

M

L

D

D

G
|
G

A

A

S

T

P

P

F

W

Q

Q

K

A

F

F

W

R

Y

L

L

V

S

L

L

L

P
|
P

M

L

L

S

V

V

G

P

I

I

L

L

S

A

V

V

L

V

F

F

L

I

L

L

R

S

F

F

I

I

E190 (= E315) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A317) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G321) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P334) mutation to A: No effect.

Query Sequence

>3607946 FitnessBrowser__Dino:3607946
MTDTPTPTPPTGRGALHRREARLAWGLLLPTVISVSLVVILPLLAIFWISFKPVGLADLR
PTAPVVRESLRGSGEDLRIEYRLRNSSQDKEIRGVTLTDTLPDTVVVGEGLPEFCTLSGR
ALRCDYGDISGGFNERFALPITLAPGADEDAVEDVVEGSLPRITGEGDSILTNAEFTLEN
FARIFDADEFWGVLGVTMFYTVFGTLGALLFGLFAALLLNKSFRGQGILRGLYLFPYVAP
VIAVAFTWVTLFDPFSGSANALLIQMGVTNEAINFFGQRPLALIMVTVFEIWRYFPLSFL
FILARMQSIDTDMYEAADMDGASPFQKFWYLSLPMLVGILSVLFLLRFIWTFNKFDDIFL
LTGGNAGTRTLTVNVYEQAFAVSNIGAGAAVAVVIFGCLLLFSVLFFRFISREEGL

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory