SitesBLAST
Comparing 3607953 Dshi_1361 D-lactate dehydrogenase (cytochrome) (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
51% identity, 94% coverage: 28:466/468 of query aligns to 17:456/456 of 8jdsA
- binding flavin-adenine dinucleotide: E32 (≠ T43), P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), W323 (≠ Y333), E414 (= E424), H415 (= H425), N451 (= N461)
- binding manganese (ii) ion: H370 (= H380), H377 (= H387), E414 (= E424)
- binding pyruvic acid: R319 (= R329), H370 (= H380), H377 (= H387), H415 (= H425)
8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
51% identity, 94% coverage: 28:466/468 of query aligns to 17:455/455 of 8jdtA
- binding 2-ketobutyric acid: R318 (= R329), H369 (= H380), H376 (= H387), H414 (= H425)
- binding flavin-adenine dinucleotide: P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), W322 (≠ Y333), E413 (= E424), H414 (= H425), N450 (= N461)
- binding manganese (ii) ion: H369 (= H380), H376 (= H387), E413 (= E424)
8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
50% identity, 94% coverage: 28:466/468 of query aligns to 17:455/455 of 8jdeA
- binding flavin-adenine dinucleotide: P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), W322 (≠ Y333), E413 (= E424), H414 (= H425), N450 (= N461)
- binding lactic acid: R318 (= R329), H369 (= H380), H376 (= H387), H414 (= H425)
- binding manganese (ii) ion: H369 (= H380), H376 (= H387), E413 (= E424)
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
51% identity, 94% coverage: 28:466/468 of query aligns to 17:456/456 of 8jdrA
- binding flavin-adenine dinucleotide: P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), Y324 (≠ W334), H370 (= H380), E414 (= E424), N451 (= N461)
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: R319 (= R329), W323 (≠ Y333), H415 (= H425)
8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid (see paper)
51% identity, 94% coverage: 28:466/468 of query aligns to 17:456/456 of 8jdqA
- binding (2R)-2-hydroxy-4-methylpentanoic acid: R319 (= R329), W323 (≠ Y333), H370 (= H380), H415 (= H425)
- binding flavin-adenine dinucleotide: P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), H370 (= H380), E414 (= E424), N451 (= N461)
8jdoA Crystal structure of h405a mldhd in complex with d-2-hydroxyhexanoic acid (see paper)
51% identity, 94% coverage: 28:466/468 of query aligns to 17:456/456 of 8jdoA
- binding (2R)-2-hydroxyhexanoic acid: R319 (= R329), W323 (≠ Y333), H415 (= H425)
- binding flavin-adenine dinucleotide: P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), Y324 (≠ W334), H370 (= H380), E414 (= E424), N451 (= N461)
8jdnA Crystal structure of h405a mldhd in complex with d-2-hydroxyvaleric acid (see paper)
51% identity, 94% coverage: 28:466/468 of query aligns to 17:456/456 of 8jdnA
- binding flavin-adenine dinucleotide: P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), H370 (= H380), E414 (= E424), N451 (= N461)
- binding (2R)-2-oxidanylpentanoic acid: R319 (= R329), W323 (≠ Y333), H415 (= H425)
8jdgA Crystal structure of h405a mldhd in complex with d-2-hydroxybutanoic acid (see paper)
51% identity, 94% coverage: 28:466/468 of query aligns to 17:456/456 of 8jdgA
- binding flavin-adenine dinucleotide: P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), H370 (= H380), E414 (= E424), N451 (= N461)
- binding (2R)-2-oxidanylbutanoic acid: R319 (= R329), H415 (= H425)
8jdbA Crystal structure of h405a mldhd in complex with d-2-hydroxyoctanoic acid (see paper)
51% identity, 94% coverage: 28:466/468 of query aligns to 17:456/456 of 8jdbA
- binding flavin-adenine dinucleotide: P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), Y324 (≠ W334), H370 (= H380), E414 (= E424), N451 (= N461)
- binding (2R)-2-oxidanyloctanoic acid: V75 (≠ L86), R319 (= R329), W323 (≠ Y333), H415 (= H425)
8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
51% identity, 94% coverage: 28:466/468 of query aligns to 17:454/454 of 8jdvA
- binding 2-Ketohexanoic acid: V75 (≠ L86), R317 (= R329), W321 (≠ Y333), H368 (= H380), H375 (= H387), H413 (= H425)
- binding flavin-adenine dinucleotide: P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), W321 (≠ Y333), Y322 (≠ W334), E412 (= E424), H413 (= H425), N449 (= N461)
- binding manganese (ii) ion: H368 (= H380), H375 (= H387), E412 (= E424)
8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
51% identity, 94% coverage: 28:466/468 of query aligns to 17:455/455 of 8jduA
- binding 2-oxopentanoic acid: R318 (= R329), W322 (≠ Y333), H369 (= H380), H376 (= H387), H414 (= H425)
- binding flavin-adenine dinucleotide: P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), W322 (≠ Y333), E413 (= E424), N450 (= N461)
- binding manganese (ii) ion: H369 (= H380), H376 (= H387), E413 (= E424)
8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
51% identity, 94% coverage: 28:466/468 of query aligns to 17:455/455 of 8jdxA
- binding flavin-adenine dinucleotide: E32 (≠ T43), P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), W322 (≠ Y333), E413 (= E424), H414 (= H425), N450 (= N461)
- binding 3-methyl-2-oxobutanoic acid: R318 (= R329), H369 (= H380), H376 (= H387), H414 (= H425)
- binding manganese (ii) ion: H369 (= H380), H376 (= H387), E413 (= E424)
8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
50% identity, 94% coverage: 28:466/468 of query aligns to 17:455/455 of 8jdpA
- binding flavin-adenine dinucleotide: P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), H369 (= H380), E413 (= E424), N450 (= N461)
- binding deaminohydroxyvaline: R319 (= R329), H414 (= H425)
8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
51% identity, 94% coverage: 28:466/468 of query aligns to 17:454/454 of 8jdzA
- binding (3S)-3-methyl-2-oxopentanoic acid: R318 (= R329), W322 (≠ Y333), H369 (= H380), H376 (= H387), H413 (= H425)
- binding flavin-adenine dinucleotide: E32 (≠ T43), P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), W322 (≠ Y333), E412 (= E424), H413 (= H425), N449 (= N461)
- binding manganese (ii) ion: H369 (= H380), H376 (= H387), E412 (= E424)
8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
51% identity, 94% coverage: 28:466/468 of query aligns to 17:454/454 of 8jdyA
- binding 2-oxo-4-methylpentanoic acid: R318 (= R329), W322 (≠ Y333), S336 (≠ V347), H369 (= H380), H376 (= H387), H413 (= H425)
- binding flavin-adenine dinucleotide: P68 (= P79), G70 (= G81), T71 (= T82), G72 (= G83), T73 (= T84), G74 (≠ S85), G78 (≠ H89), V79 (= V90), L90 (≠ F101), P132 (= P143), G133 (= G144), A134 (= A145), G140 (= G151), M141 (= M152), A143 (= A154), T144 (= T155), A146 (= A157), S147 (= S158), E200 (= E208), G201 (= G209), I206 (= I214), E412 (= E424), N449 (= N461)
- binding manganese (ii) ion: H369 (= H380), H376 (= H387), E412 (= E424)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
30% identity, 94% coverage: 28:467/468 of query aligns to 22:465/467 of 7qh2C
- binding flavin-adenine dinucleotide: V73 (≠ P79), G75 (= G81), S76 (≠ T82), G77 (= G83), T78 (= T84), G79 (≠ S85), L80 (= L86), A83 (≠ H89), C84 (≠ V90), P137 (= P143), G138 (= G144), E139 (vs. gap), A142 (= A147), T143 (≠ S148), G146 (= G151), N147 (≠ M152), S149 (≠ A154), T150 (= T155), A152 (= A157), G153 (≠ S158), E203 (= E208), G204 (= G209), I209 (= I214), E422 (= E424), H423 (= H425)
- binding fe (iii) ion: H377 (= H380), H384 (= H387), E422 (= E424)
P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
34% identity, 94% coverage: 27:466/468 of query aligns to 15:456/459 of P9WIT1
- K354 (≠ E359) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
31% identity, 87% coverage: 57:464/468 of query aligns to 53:462/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R329), T337 (≠ Y333), K348 (≠ V347), Y379 (≠ L378), H381 (= H380), H388 (= H387), H423 (= H425)
- binding flavin-adenine dinucleotide: P75 (= P79), Q76 (≠ F80), G77 (= G81), G78 (≠ T82), N79 (≠ G83), T80 (= T84), G81 (≠ S85), M82 (≠ L86), G85 (≠ H89), S86 (≠ V90), L139 (≠ P143), G140 (= G144), A141 (= A145), C145 (vs. gap), G149 (= G151), N150 (≠ M152), A152 (= A154), T153 (= T155), G157 (= G159), G207 (= G209), I212 (= I214), E422 (= E424), N459 (= N461)
- binding zinc ion: H381 (= H380), H388 (= H387), E422 (= E424)
Sites not aligning to the query:
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
31% identity, 87% coverage: 57:464/468 of query aligns to 53:462/466 of 6lpuA
- binding flavin-adenine dinucleotide: P75 (= P79), G77 (= G81), G78 (≠ T82), N79 (≠ G83), T80 (= T84), G81 (≠ S85), G85 (≠ H89), S86 (≠ V90), L139 (≠ P143), G140 (= G144), A141 (= A145), C145 (vs. gap), H146 (vs. gap), G148 (= G150), G149 (= G151), N150 (≠ M152), A152 (= A154), T153 (= T155), A155 (= A157), E206 (= E208), G207 (= G209), I211 (≠ V213), I212 (= I214), E422 (= E424), N459 (= N461)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R329), T337 (≠ Y333), K348 (≠ V347), Y379 (≠ L378), H381 (= H380), H388 (= H387), H423 (= H425)
- binding zinc ion: H381 (= H380), H388 (= H387), E422 (= E424)
Sites not aligning to the query:
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
31% identity, 87% coverage: 57:464/468 of query aligns to 53:462/466 of 6lpqA
- binding flavin-adenine dinucleotide: P75 (= P79), G77 (= G81), G78 (≠ T82), N79 (≠ G83), T80 (= T84), G81 (≠ S85), G85 (≠ H89), S86 (≠ V90), L139 (≠ P143), G140 (= G144), A141 (= A145), C145 (vs. gap), H146 (vs. gap), G149 (= G151), N150 (≠ M152), A152 (= A154), T153 (= T155), A155 (= A157), G157 (= G159), E206 (= E208), G207 (= G209), I211 (≠ V213), I212 (= I214), E422 (= E424), N459 (= N461)
- binding d-malate: M82 (≠ L86), R333 (= R329), T337 (≠ Y333), K348 (≠ V347), Y379 (≠ L378), H381 (= H380), H388 (= H387), E422 (= E424), H423 (= H425)
- binding zinc ion: H381 (= H380), H388 (= H387), E422 (= E424)
Sites not aligning to the query:
Query Sequence
>3607953 Dshi_1361 D-lactate dehydrogenase (cytochrome) (RefSeq)
MTDQTPLRRNAAGIATALDVLRQKFGARLQTGAALREQHGHTTTWLPNQPPDAVFLPRET
AEVQDVLRICTAYGMPLVPFGTGTSLEGHVNAPLGGLSLDFAEMNQVLAIHPEDMDCVVQ
PGITRKALNEELRATGLFFPVDPGADASLGGMAATRASGTNAVRYGTMRDVVLALEAVTA
DGRVIRTAKRARKTSAGYDLTRLLVGSEGTLGVITELTLKLSGIPEAVAGATCSFASVEA
ACAAVIATIQYGVPVARIEFLDALSVRSINAYSKLSLPEAPLLLLEFHGSEAGVAEQSET
FGEIAADHGALAFDWTSDAEARAKMWQARHDAYWAMRALRPGFDAFVTDVCVPVSRLAEA
VSAAAERAEADGIIAPTLGHVGDGNFHVALLIDPDDPGGRARAESYIGWLNDLAISLDGT
CTGEHGIGQGKVKYLARELGANTVDVMAALKSAMDPDNILNPGKLFQG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory