SitesBLAST

269:273 binding
294 active site, 5-glutamyl coenzyme A thioester intermediate; E→A: Abolishes enzyme activity.
357 C→Y: Strongly impaired protein solubility.

4eu6A Succinyl-coa:acetate coa-transferase (aarch6) in complex with coa, acetate, and covalent acetylglutamyl anhydride and glutamyl-coa thioester adducts (see paper)
28% identity, 25% coverage: 377:503/515 of query aligns to 357:492/513 of 4eu6A

query
sites
4eu6A

V

I

M

A

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M

L

N

G

G

A
x
M

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x
I

E

E

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D

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Y

F

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N

N

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Y

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-
x
A

-

K

G

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I

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H

binding coenzyme a: M362 (≠ A382), I363 (≠ V383), N383 (≠ G403), G384 (= G404), I385 (≠ P405), S388 (≠ H408), A406 (vs. gap), I411 (≠ F429)

Sites not aligning to the query:

binding acetate ion: 91, 113, 293
binding coenzyme a: 268, 269, 271, 293

4eu5A Succinyl-coa:acetate coa-transferase (aarch6) in complex with coa (see paper)
28% identity, 25% coverage: 377:503/515 of query aligns to 357:492/513 of 4eu5A

query
sites
4eu5A

V

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binding coenzyme a: M362 (≠ A382), I363 (≠ V383), M382 (≠ L402), N383 (≠ G403), G384 (= G404), S388 (≠ H408), A406 (vs. gap), K407 (vs. gap), I411 (≠ F429)

Sites not aligning to the query:

binding coenzyme a: 293

4eu3B Succinyl-coa:acetate coa-transferase (aarch6) in complex with citrate (subunit b) or unliganded (subunit a) (see paper)
28% identity, 25% coverage: 377:503/515 of query aligns to 357:492/504 of 4eu3B

query
sites
4eu3B

V

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G

G

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H

Sites not aligning to the query:

binding citric acid: 35, 70, 93, 227, 230

5dw5A Succinyl-coa:acetate coa-transferase (aarch6) bound to the coa analogue 3'-phosphoadenosine 5'-(o-(n-propylpantothenamide)) pyrophosphate (mx) (see paper)
28% identity, 25% coverage: 377:503/515 of query aligns to 356:491/512 of 5dw5A

query
sites
5dw5A

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M

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x
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x
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S

V

W

H

H

binding [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl][(3R)-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-4-[[3-oxidanylidene-3-(propylamino)propyl]amino]butyl] hydrogen phosphate: N359 (≠ L380), M361 (≠ A382), I362 (≠ V383), G383 (= G404), G386 (= G407), S387 (≠ H408), A405 (vs. gap), I410 (≠ F429)

Sites not aligning to the query:

4eucA Succinyl-coa:acetate coa-transferase (aarch6-e294a) in complex with dethiaacetyl-coa (see paper)
28% identity, 25% coverage: 377:503/515 of query aligns to 357:492/504 of 4eucA

query
sites
4eucA

V

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M

A

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M

L
x
N

G

G

A
x
M

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x
I

E

E

V

A

D

D

F

I

G

Y

F

G

N

N

V

V

N

N

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S

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D

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F

A

A

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R

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x
A

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x
I

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E

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G

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A

A

-

D

-

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R

-

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A

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L

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H

H

binding [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3R)-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-4-[[3-oxidanylidene-3-(4-oxidanylidenepentylamino)propyl]amino]butyl] hydrogen phosphate: N360 (≠ L380), M362 (≠ A382), I363 (≠ V383), M382 (≠ L402), N383 (≠ G403), G384 (= G404), G386 (= G406), G387 (= G407), S388 (≠ H408), A406 (vs. gap), K407 (vs. gap), I411 (≠ F429)

Sites not aligning to the query:

binding [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3R)-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-4-[[3-oxidanylidene-3-(4-oxidanylidenepentylamino)propyl]amino]butyl] hydrogen phosphate: 268, 269, 270, 271, 272

Query Sequence

>3608020 FitnessBrowser__Dino:3608020
MRGVSVTPQGSILDTARWPAGAAPVTTPLGLLLPVGNPAPALSVLPPRADAGDGRKLRPG
LEVVFDELGIADGRTLSFHHHYRDGDRVVVEVMRLARERGLKGLTICPSSIFPTHSSLVP
LLEDGTITSIVTDYMRGPVADWITAHPGRVTVLLQSHGGRARAISSGQLKIDVAFVGASL
ADRRGNLTGRAGALACGPLGYPAVDAQYAKATVGMAHDVTDAALPRVDIPARFVDLVVPF
ERPGDANRIRSGSTVPSETPLSRGIAERSSDIIAAAGLLTEEFALQSGAGGYSLAAVPHV
GSLLAAQGMRGAFMSGGITSAHVELLRAGVFRAIRDVQCFDLEAVRSAIENPDHQMMTAA
EYASPLHPNPCVDDLSVMLLGAVEVDFGFNVNVVSGTDGRILGGPGGHPDAARGADLSIV
LTSLTGGGFAKIVPAVRCVSTPGVDVDVVVTEAGFAINPARADLVARLTRAGLKPVEIED
LARIAGAQAAHSPAPLAERPSVHIERRDGLILDWV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory