SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3608039 Dshi_1446 aminotransferase class-III (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5lh9D Amine transaminase crystal structure from an uncultivated pseudomonas species in the plp-bound (internal aldimine) form
38% identity, 97% coverage: 4:435/444 of query aligns to 14:445/449 of 5lh9D

query
sites
5lh9D

H

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active site: Y148 (= Y132), D255 (= D240), K284 (= K269), T321 (= T303)
binding pyridoxal-5'-phosphate: G114 (= G98), G115 (= G99), S116 (= S100), Y148 (= Y132), H149 (= H133), G150 (= G134), E222 (= E206), D255 (= D240), V257 (= V242), V258 (≠ M243), K284 (= K269), Y320 (≠ H302), T321 (= T303)

5lhaA Amine transaminase crystal structure from an uncultivated pseudomonas species in the pmp-bound form
38% identity, 97% coverage: 4:435/444 of query aligns to 12:443/447 of 5lhaA

query
sites
5lhaA

H

H

V

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active site: Y146 (= Y132), D253 (= D240), K282 (= K269), T319 (= T303)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G112 (= G98), G113 (= G99), S114 (= S100), Y146 (= Y132), H147 (= H133), G148 (= G134), E220 (= E206), D253 (= D240), V255 (= V242), V256 (≠ M243), K282 (= K269), Y318 (≠ H302), T319 (= T303)

6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
38% identity, 95% coverage: 17:439/444 of query aligns to 35:447/448 of 6io1B

query
sites
6io1B

V

V

A

R

G

G

A

E

G

G

C

V

Y

W

I

V

I

W

D

D

A

A

D

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G

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K

K

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Y

Y

L

L

D

D

G

G

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A

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W

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V

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A

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F

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F

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G

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A

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N

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K

D

A

T

S

K

K

R

E

P

K

L

F

D

P

P

P

A

E

R

F

K

K

V

L

S

G

A

P

R

K

I

L

K

E

A

A

A

A

A

T

M

R

A

R

R

R

G

G

L

I

I

I

-

V

-

R

C

C

Y

T

P

P

G

-

S

-

G

-

T

-

I

-

D

-

G

-

K

-

H

-

G

-

D

D

H

G

I

I

L

V

L

M

A

A

P

P

P

P

F

L

I

T

I

I

E

S

D

R

K

A

Q

E

I

C

D

D

A

V

L

L

V

I

H

E

M

G

L

V

A

A

E

A

A

A

I

L

D

S

D

D

A

V

M

L

active site: Y151 (= Y132), D257 (= D240), K286 (= K269)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G98), G118 (= G99), A119 (≠ S100), N122 (≠ V103), Y151 (= Y132), H152 (= H133), G153 (= G134), E224 (= E206), D257 (= D240), V259 (= V242), I260 (≠ M243), K286 (= K269)

Sites not aligning to the query:

active site: 20

5kr6B Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
34% identity, 98% coverage: 4:436/444 of query aligns to 23:454/460 of 5kr6B

query
sites
5kr6B

H

H

V

L

F

R

G

D

R

H

S

A

A

S

A

G

Q

E

M

L

P

P

T

G

A

R

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I

-

I

-

T

A

G

G

G

A

K

G

G

C

I

Y

R

I

I

I

Q

D

D

A

S

D

E

G

G

K

R

R

E

Y

Y

L

I

D

D

G

A

S

F

G

A

G

G

A

L

A

Y

V

C

S

V

C

N

L

V

G

G

H

Y

D

G

H

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P

T

A

E

V

I

R

A

A

D

A

A

L

I

H

Y

A

E

Q

Q

I

A

D

K

K

E

I

L

A

A

F

Y

A

Y

H

H

T

T

G

Y

F

V

-

G

F

H

T

S

S

N

E

E

P

P

A

I

E

I

V

E

L

L

C

S

D

E

A

R

L

I

I

I

-

R

A

E

A

W

A

A

P

P

K

A

G

G

I

M

E

S

K

K

V

V

Y

Y

L

Y

L

G

S

M

G
x
S

G
|
G

S
|
S

E

D

S

A

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x
N

E

E

A

T

A

Q

L

I

K

K

L

L

A

V

R

W

Q

Y

F

Y

F

N

L

N

E

V

T

L

G

G

E

R

P

P

R

Q

R

K

H

K

R

K

V

I

I

I

A

S

R

R

R

Q

Q

R

S

G

Y
|
Y

H
|
H

G
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G

N

S

T

G

L

I

-

M

-

T

G

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L

L

A

T

A

-

G

G

G

L

N

P

A

A

W

F

R

H

R

N

E

H

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F

Y

D

A

L

P

P

L

L

L

-

V

-

E

E

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P

Y

I

H

R

I

H

D

T

P

T

C

C

Y

P

A

H

Y

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Y

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Q

A

A

P

V

A

G

G

E

M

S

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E

A

A

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E

Y

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R

R

H

A

C

A

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D

D

A

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E

M

I

I

E

L

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A

L

E

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G

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D

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T

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M

A

A

A

F

F

I

I

A

G

E
|
E

P

P

V

V

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M

G

G

A

-

T

T

A

G

G

G

A

I

V

V

P

P

P

P

A

P

P

E

G

G

Y

Y

F

W

E

E

R

A

I

I

R

Q

E

A

I

V

C

L

D

N

E

K

Y

Y

G

D

V

I

L

L

L

L

I

I

L

A

D
|
D

E

E

V
|
V

M
x
V

C

C

G

G

M

F

G

G

R

R

T

T

G

G

T

S

L

M

F

F

A

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E

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Q

H

D

Y

G

G

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P

P

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D

I

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A

A

K
|
K

G

G

L

L

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T

A

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G

A

Y

Y

A

A

P

P

I

L

G

S

A

G

M

V

L

I

A

V

S

G

G

E

R

K

I

V

Y

W

D

K

A

V

I

L

A

E

Q

Q

G

G

S

S

-

D

-

Q

-

Y

G

G

S

P

F

I

Q

G

H

H

G

G

H
x
W

T
|
T

Y

Y

H

S

G

G

H

H

P

P

L

I

A

C

A

A

A

A

A

A

A

A

G

L

A

A

V

N

V

L

E

D

T

I

L

I

R

E

A

R

P

E

G

N

T

L

M

T

A

G

Q

N

V

A

R

A

A

D

K

T

G

G

A

A

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Y

L

F

Q

Q

S

Q

R

R

L

M

E

R

A

E

A

T

L

F

G

G

Q

D

H

H

S

P

H

L

V

V

G

G

D

E

I

V

R

R

G

G

R

V

G

G

L

L

F

M

R

A

G

A

I

L

E

E

L

F

V

V

E

A

D

D

R

K

D

D

T

K

K

R

R

T

P

R

L

F

D

D

P

P

A

S

R

L

K

K

V

V

S

G

A

P

R

R

I

V

K

S

A

A

A

A

A

C

M

L

A

E

R

D

G

G

L

M

I

I

C

A

Y

R

P

A

G

M

S

P

G

-

T

-

I

-

D

-

G

-

K

-

H

H

G

G

D

D

H

I

I

L

L

G

L

F

A

A

P

P

P

P

F

L

I

V

I

I

E

T

D

R

K

A

Q

E

I

V

D

D

A

E

L

I

V

V

H

D

M

I

L

A

A

K

E

Q

A

A

I

V

D

D

binding pyridoxal-5'-phosphate: S121 (≠ G98), G122 (= G99), S123 (= S100), N126 (≠ V103), Y155 (= Y132), H156 (= H133), G157 (= G134), E228 (= E206), D261 (= D240), V263 (= V242), V264 (≠ M243), K290 (= K269), W326 (≠ H302), T327 (= T303)

6fyqA The crystal structure of a new transaminase from the marine bacterium virgibacillus (see paper)
35% identity, 94% coverage: 19:435/444 of query aligns to 33:435/443 of 6fyqA

query
sites
6fyqA

G

G

A

D

G

G

C

I

Y

Y

I

L

I

T

D

D

A

V

D

T

G

G

K

K

R

T

Y

Y

L

I

D

D

G

G

S

V

G

S

G

S

A

L

A

W

V

N

S

V

C

N

L

V

G

G

H

H

D

G

H

R

P

V

A

E

V

L

R

A

A

E

A

A

L

A

H

A

A

Q

Q

Q

I

M

D

K

K

K

I

M

A

A

F

F

A

S

H

S

T

A

-

F

G

S

F

T

F

F

T

S

S

H

E

E

P

P

A

A

E

I

V

R

L

L

C

A

D

E

A

K

L

I

I

A

A

S

A

I

A

T

P

P

K

E

G

G

I

L

E

N

K

A

V

V

Y

F

L

F

L

T

S

S

G
|
G

G
|
G

S
|
S

E

E

S

S

V
x
N

E

D

A

S

A

A

L

V

K

K

L

L

A

V

R

R

Q

H

F

Y

F

W

L

K

E

I

T

Q

G

G

E

K

P

P

R

N

R

K

H

R

R

K

V

I

I

I

A

S

R

L

R

K

Q

R

S

S

Y
|
Y

H
|
H

G
|
G

N

V

T

A

L

A

G

A

A

S

L

T

A

S

A

V

G

T

G

G

N

I

A

P

W

E

R

F

R

W

E

G

K

M

Y

A

A

G

P

H

L

M

L

M

V

T

E

D

T

F

Y

L

H

H

I

V

D

D

P

T

C

H

Y

Y

A

N

Y

N

R

T

H

T

Q

E

A

-

V

-

G

-

E

-

S

-

D

-

A

-

A

-

Y

-

G

-

R

-

R

Q

A

A

A

V

D

Q

A

S

L

L

R

C

T

Q

E

A

I

I

E

E

R

E

L

A

G

G

P

P

E

E

T

T

V

I

M

A

A

A

F

F

I

F

A

A

E
|
E

P

P

V

V

V

Q

G

G

A

A

T

-

A

G

G

G

A

V

V

I

P

I

P

P

A

P

P

E

G

D

Y

Y

F

F

E

L

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E

E

I

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C

C

D

N

E

A

Y

Y

G

G

V

I

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L

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F

I

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A

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|
D

E

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M
x
I

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G

G

M

F

G

G

R

R

T

T

G

G

T

K

L

M

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I

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M

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I

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|
K

G

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T

A

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G

G

Y

Y

A

F

P

P

I

L

G

G

A

G

M

V

L

V

A

V

S

S

G

D

R

P

I

I

Y

H

D

E

A

V

I

L

A

K

Q

E

G

K

S

S

-

V

G

G

S

T

F

L

Q

F

H

H

G

G

H

F

T

T

Y

Y

H

S

G

G

H

H

P

P

L

T

A

A

A

A

A

A

A

V

A

A

G

L

A

K

V

N

V

I

E

A

T

I

L

I

R

K

A

E

P

E

G

R

T

L

M

V

A

E

Q

N

V

S

R

K

A

R

K

M

G

G

A

D

T

A

L

L

Q

L

S

H

R

G

L

L

E

K

A

K

A

V

L

K

G

N

Q

R

H

L

S

E

H

I

V

V

G

G

D

D

I

V

R

R

G

F

R

V

G

G

L

L

F

L

R

G

G

A

I

V

E

E

L

L

V

M

E

Q

D

N

R

P

D

A

T

T

K

N

R

K

P

P

L

F

D

S

P

S

A

N

R

L

K

Q

V

V

S

A

A

P

R

K

I

V

K

I

A

E

A

A

A

L

M

H

A

E

R

L

G

G

L

V

I

I

C

C

Y

R

P

-

G

-

S

S

G

V

T

T

I

Y

D

D

G

-

K

-

H

H

G

T

D

N

H

I

I

I

L

C

L

L

A

A

P

P

P

P

F

L

I

I

I

I

E

N

D

Q

K

K

Q

Q

I

V

D

D

A

K

L

L

V

V

H

E

M

V

L

I

A

Y

E

E

A

A

I

I

active site: Y147 (= Y132), D243 (= D240), K272 (= K269)
binding pyridoxal-5'-phosphate: G113 (= G98), G114 (= G99), S115 (= S100), N118 (≠ V103), Y147 (= Y132), H148 (= H133), G149 (= G134), E210 (= E206), D243 (= D240), V245 (= V242), I246 (≠ M243), K272 (= K269)

Sites not aligning to the query:

active site: 15

5kr5A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
34% identity, 98% coverage: 4:437/444 of query aligns to 19:451/455 of 5kr5A

query
sites
5kr5A

H

H

V

A

F

Y

G

D

R

H

S

A

A

S

A

G

Q

A

M

L

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G

A

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-

I

-

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A

G

G

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A

K

G

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R

I

I

I

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D

D

A

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G

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A

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G

A

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I

G

G

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Y

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G

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P
x
E

A

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A

A
x
D

A

A

L

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H

Y

A

E

Q

Q

I

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D

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K

Q

I

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A

A

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Y

A

Y

H

H

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G

Y

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S

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x
N

E

E

A

T

A

Q

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K

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F

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|
Y

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H

G
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A

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A

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A

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L

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K

E

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I

I

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A

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I

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A

A

A

F

F

I

I

A

A

E
|
E

P

P

V

V

V

M

G

G

A

-

T

T

A

G

G

G

A

I

V

V

P

P

P

P

A

P

P

Q

G

G

Y

Y

F

W

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I

I

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A

I

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C

L

D

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Y

H

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D

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I

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L

L

L

I

I

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A

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D

E

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V

M

V

C

C

G

G

M

F

G

G

R

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G

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L

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F

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A

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E

E

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Y

G

G

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P

P

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D

I

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V

I

T

T

I

V

A

A

K
|
K

G

G

L

L

G

T

A

S

G

A

Y

Y

A

A

P

P

I

L

G

S

A

A

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L

I

A

V

S

S

G

E

R

K

I

V

Y

W

D

D

A

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I

I

A

E

Q

K

G

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S

-

R

-

E

-

H

G

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Q

G

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H

G

G

H
x
W

T
|
T

Y

Y

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P

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A

C

A

A

A

A

A

A

A

A

G

L

A

A

V

N

V

L

E

D

T

I

L

L

R

E

A

R

P

E

G

N

T

L

M

T

A

G

Q

N

V

A

R

A

A

D

K

V

G

G

A

A

T

Y

L

L

Q

Q

S

Q

R

R

L

L

E

H

A

E

A

A

L

F

G

G

Q

A

H

H

S

P

H

L

V

V

G

G

D

E

I

V

R

R

G

G

R

V

G

G

L

M

F

L

R

A

G

A

I

L

E

E

L

F

V

M

E

A

D

D

R

K

D

G

T

A

K

R

R

T

P

P

L

F

D

D

P

P

A

A

R

L

K

K

V

V

S

S

A

Q

R

K

I

V

K

A

A

A

A

A

A

A

M

L

A

E

R
x
D

G

G

L

L

I

I

C

V

Y

R

P

A

G

L

S

P

G

-

T

-

I

-

D

-

G

-

K

-

H

H

G

G

D

D

H

I

I

L

L

G

L

F

A

A

P

P

P

P

F

L

I

V

I

T

E

T

D

R

K

A

Q

E

I

V

D

D

A

E

L

I

V

V

H

A

M

I

L

A

A

K

E
|
E

A

A

I

F

D

D

D

E

binding calcium ion: E66 (≠ P49), D70 (≠ A53), D412 (≠ R392), E447 (= E433)
binding pyridoxal-5'-phosphate: S117 (≠ G98), G118 (= G99), S119 (= S100), N122 (≠ V103), Y151 (= Y132), H152 (= H133), G153 (= G134), E224 (= E206), D257 (= D240), V259 (= V242), K286 (= K269), W322 (≠ H302), T323 (= T303)

6s54A Transaminase from pseudomonas fluorescens (see paper)
34% identity, 95% coverage: 12:435/444 of query aligns to 29:448/453 of 6s54A

query
sites
6s54A

Q

Q

M

R

P

P

T

L

A

I

V

I

-

Q

A

S

G

G

A

K

G

G

C

V

Y

H

I

I

I

T

D

D

A

V

D

D

G

G

K

R

R

R

Y

F

L

I

D

D

G

C

S

Q

G

G

G

G

A

L

A

W

V

C

S

V

C

N

L

A

G

G

H

Y

D

G

H

R

P

R

A

E

V

I

R

I

A

D

A

A

L

V

H

T

A

R

Q

Q

I

M

D

E

K

E

I

L

A

A

F

Y

A

-

H

Y

T

S

G

L

F

F

-

P

-

G

F

S

T

T

S

N

E

A

P

P

A

A

E

I

V

A

L

L

C

S

D

Q

A

K

L

L

I

T

-

E

A

V

A

A

A

A

P

E

K

E

G

G

I

M

E

V

K

K

V

A

Y

S

L

F

L

G

S

L

G
|
G

G
|
G

S
|
S

E

D

S

A

V

V

E

E

A

T

A

A

L

L

K

K

L

I

A

A

R

R

Q

Q

F

Y

F

W

L

K

E

L

T

E

G

G

E

Q

P

P

R

D

R

K

H

V

R

K

V

F

I

V

A

S

R

L

R

Y

Q

N

S

G

Y
|
Y

H
|
H

G
|
G

N

L

T

N

L

F

G

G

A

G

L

M

A

S

A

A

-

C

G

G

G

G

N

N

A

A

W

W

R

K

R

-

E

S

K

S

Y

Y

A

E

P

P

L

L

L

M

V

P

E

G

T

F

Y

F

H

Q

I

V

D

E

P

S

C

P

Y

H

A

L

Y

Y

R

R

H

N

Q

P

A

F

V

T

G

N

E

D

S

P

D

E

A

E

A

-

Y

L

G

A

R

E

R

I

A

C

A

A

D

Q

A

I

L

L

R

E

T

R

E

Q

I

I

E

E

R

M

L

Q

G

A

P

P

E

G

T

T

V

V

M

A

A

A

F

L

I

I

A

A

E
|
E

P

P

V

I

V

Q

G

G

A

A

T

G

A

G

G

V

A

I

V

V

P

P

P

P

A

A

P

-

G

S

Y

Y

F

W

E

P

R

R

I

L

R

R

E

Q

I

I

C

C

D

D

E

K

Y

Y

G

D

V

I

L

L

L

L

I

I

L

A

D
|
D

E

E

V
|
V

M
x
I

C

T

G

G

M

L

G

G

R

R

T

S

G

G

T

S

L

L

F

F

A

G

C

S

E

R

Q

G

D

W

G

G

I

V

S

K

P

P

D

D

I

I

V

M

T

C

I

L

A

A

K
|
K

G

G

L

I

G

S

A

S

G

G

Y

Y

A

V

P

P

I

L

G

S

A

A

M

T

L

L

A

V

S

N

G

S

R

R

I

V

Y

A

D

R

A

A

I

W

A

E

Q

R

G

D

S

A

G

G

-

F

-

T

-

S

S

V

F

Y

Q

M

H

H

G

G

H
x
Y

T
|
T

Y

Y

H

S

G

G

H

H

P

P

L

V

A

S

A

C

A

A

A

A

A

A

G

L

A

A

V

A

V

I

E

D

T

I

L

V

R

L

A

Q

P

E

G

N

T

L

M

A

A

E

Q

N

V

A

R

R

A

V

K

V

G

G

A

D

T

Y

L

F

Q

L

S

E

R

K

L

L

E

L

A

I

A

L

L

K

G

D

Q

K

H

H

S

R

H

A

V

I

G

G

D

D

I

V

R

R

G

G

R

K

G

G

L

L

F

M

R

L

G

A

I

V

E

E

L

L

V

V

E

K

D

E

R

R

D

A

T

T

K

K

R

E

P

P

L

F

D

G

P

P

A

A

R

D

K

A

V

Y

S

P

A

L

R

A

I

I

K

S

A

E

A

A

A

C

M

V

A

N

R

N

G

G

L

V

I

M

C

I

Y

R

P

-

G

-

S

-

G

-

T

T

I

I

D

V

G

N

K

K

H

-

G

-

D

-

H

-

I

L

L

I

L

I

A

S

P

P

P

P

F

L

I

T

I

F

E

T

D

T

K

E

Q

H

I

V

D

D

A

E

L

V

V

I

H

E

M

V

L

L

A

D

E

R

A

A

I

F

active site: Y152 (= Y132), D258 (= D240), K287 (= K269)
binding pyridoxal-5'-phosphate: G118 (= G98), G119 (= G99), S120 (= S100), Y152 (= Y132), H153 (= H133), G154 (= G134), E225 (= E206), D258 (= D240), V260 (= V242), I261 (≠ M243), K287 (= K269), Y323 (≠ H302), T324 (= T303)

Sites not aligning to the query:

active site: 20

6gwiB The crystal structure of halomonas elongata amino-transferase (see paper)
35% identity, 94% coverage: 21:438/444 of query aligns to 37:445/450 of 6gwiB

query
sites
6gwiB

G

G

C

V

Y

Y

I

I

I

H

D

D

A

S

D

E

G

G

K

N

R

R

Y

I

L

L

D

D

G

G

S

M

G

A

G

G

A

L

A

W

V

C

S

V

C

N

L

L

G

G

H

Y

D

G

H

R

P

R

A

E

V

L

R

V

A

E

A

A

L

A

H

T

A

A

Q

Q

I

L

D

E

K

Q

I

L

A

P

F

Y

A

Y

H

N

T

T

G

F

F

F

F

K

T

T

S

T

E

H

P

P

A

P

E

A

V

V

-

R

L

L

C

A

D

E

A

K

L

L

I

C

A

D

A

L

A

A

P

P

K

A

G

H

I

I

E

N

K

R

V

V

Y

F

L

F

L

T

S

G

G
x
S

G
|
G

S
|
S

E

E

S

A

V
x
N

E

D

A

T

A

V

L

L

K

R

L

M

A

V

R

R

Q

R

F

Y

F

W

L

A

E

L

T

K

G

G

E

Q

P

P

R

D

R

K

H

Q

R

W

V

I

I

I

A

G

R

R

R

E

Q

N

S

A

Y
|
Y

H
|
H

G
|
G

N

S

T

T

L

L

G

A

A

G

L

M

A

S

A

L

G

G

G

G

N

M

A

A

W

P

R

M

R

H

E

A

K

Q

Y

G

A

G

P

P

L

C

L

V

V

P

E

G

T

I

Y

A

H

H

I

I

D

R

P

Q

C

P

Y

Y

A

W

Y

F

R

-

H

G

Q

E

A

G

V

R

G

D

E

M

S

S

D

P

A

E

A

A

Y

F

G

G

R

Q

R

T

A

C

A

A

D

E

A

A

L

L

R

E

T

E

E

K

I

I

E

L

R

E

L

L

G

G

P

E

E

E

T

K

V

V

M

A

A

A

F

F

I

I

A

A

E
|
E

P

P

V

V

V

Q

G

G

A

A

T

-

A

G

G

G

A

A

V

I

P

M

P

P

A

P

P

E

G

S

Y

Y

F

W

E

P

R

A

I

V

R

K

E

K

I

V

C

L

D

A

E

K

Y

Y

G

D

V

I

L

L

L

L

I

V

L

A

D
|
D

E

E

V
|
V

M
x
I

C

C

G

G

M

F

G

G

R

R

T

L

G

G

T

E

L

W

F

F

A

G

C

S

E

Q

Q

H

D

Y

G

G

I

L

S

E

P

P

D

D

I

L

V

M

T

P

I

I

A

A

K
|
K

G

G

L

L

G

S

A

S

G

G

Y

Y

A

L

P

P

I

I

G

G

A

G

M

V

L

L

A

V

S

G

G

D

R

R

I

V

Y

A

D

E

A

T

I

L

A

I

Q

E

G

E

S

G

G

G

S

E

F

F

Q

F

H

H

G

G

H
x
F

T
|
T

Y

Y

H

S

G

G

H

H

P

P

L

T

A

C

A

A

A

A

A

V

A

A

G

L

A

K

V

N

V

L

E

E

T

L

L

L

R

E

A

A

P

E

G

G

T

V

M

V

A

D

Q

R

V

V

R

R

A

D

K

D

G

L

A

G

T

P

L

Y

Q

L

S

A

R

E

L

R

E

W

A

A

A

S

L

L

G

V

Q

D

H

H

S

P

H

I

V

V

G

G

D

E

I

A

R

R

G

S

R

L

G

G

L

L

F

M

R

G

G

A

I

L

E

E

L

L

V

V

E

A

D

D

R

K

D

T

T

T

K

G

R

Q

P

R

L

F

D

D

P

K

A

S

R

-

K

-

V

-

S

-

A

-

R

-

I

L

K

G

A

A

A

G

A

N

M

L

A

C

R

R

G

D

L

L

I

-

C

C

Y

F

P

-

G

A

S

N

G

G

T

L

I

V

D

M

G

R

K

S

H

V

G

G

D

D

H

T

I

M

L

I

L

I

A

S

P

P

P

P

F

L

I

V

I

I

E

R

D

R

K

E

Q

E

I

I

D

D

A

E

L

L

V

V

H

E

M

L

L

A

A

R

E

R

A

A

I

L

D

D

D

E

A

T

active site: Y149 (= Y132), D255 (= D240), K284 (= K269)
binding pyridoxal-5'-phosphate: S115 (≠ G98), G116 (= G99), S117 (= S100), N120 (≠ V103), Y149 (= Y132), H150 (= H133), G151 (= G134), E222 (= E206), D255 (= D240), V257 (= V242), I258 (≠ M243), K284 (= K269), F317 (≠ H302), T318 (= T303)

Sites not aligning to the query:

active site: 18

Q94CE5 Gamma-aminobutyrate transaminase POP2, mitochondrial; AtGABA-T; Gamma-aminobutyric acid aminotransferase 1; Protein HEXENAL RESPONSE 1; Protein POLLEN-PISTIL INCOMPATIBILITY 2; AtPOP2; EC 2.6.1.96 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
33% identity, 96% coverage: 8:435/444 of query aligns to 64:488/504 of Q94CE5

query
sites
Q94CE5

R

Q

S

S

A

A

A

D

Q

L

M

D

P

P

T

L

A

V

V

I

A

A

G

K

A

S

-

E

G

G

C

S

Y

Y

I

V

I

Y

D

D

A

D

D

T

G

G

K

K

R

K

Y

Y

L

L

D

D

G

S

S

L

G

A

G

G

A

L

A

W

V
x
C

S

T

C

A

L

L

G

G

H
x
G

D

N

H

E

P

P

A

R

V

L

R

V

A

S

A

A

L

A

H

V

A

E

Q

Q

I

L

D

N

K

T

I

L

A

P

F

F

A

Y

H

H

T

S

G

F

F

W

F

N

-

R

T

T

S

T

E

K

P

P

-

S

-

L

-

D

-

L

A

A

E

K

V

V

L

L

C

L

D

E

A

M

L

F

I

T

A

A

A

-

A

-

P

-

K

N

G

K

I

M

E

A

K

K

V

A

Y

F

L

F

L

T

S

S

G

G

G

G

S

S

E

D

S

A

V

N

E

D

A

T

A

Q

L

V

K

K

L

L

A

V

R

W

Q

Y

F

Y

F

N

L

N

E

A

T

L

G

G

E

R

P

P

R

E

R

K

H

K

R

K

V

F

I

I

A

A

R

R

R

K

Q

K

S

S

Y

Y

H

H

G

G

N

S

T

T

L

L

G

I

A

S

L

A

A

S

A

L

G

S

G

G

N

L

A

P

W

P

R

L

R

H

E

Q

K

N

Y

F

-

D

-

L

-

P

A

A

P

P

L

F

L

V

V

L

E

-

T

-

Y

-

H

H

I

T

D

D

P

C

C

P

Y

H

A

Y

Y

W

R

R

H

F

Q

H

A

L

V

P

G

G

E

E

S

T

D

E

A

E

A

E

Y

F

G

S

R

T

R

R

A

L

A

A

D

K

A

N

L

L

R

E

T

D

E

L

I

I

E

I

R

K

L

E

G

G

P

P

E

E

T

T

V

I

M

G

A

A

F

F

I

I

A

A

E

E

P

P

V

V

V

M

G

G

A

A

T

-

A
x
G

G

G

A

V

V

I

P

P

P

P

A

P

P

A

G

T

Y

Y

F

F

E

E

R

K

I

V

R

Q

E

A

I

V

C

V

D

K

E

K

Y

Y

G

D

V

I

L

L

L

F

I

I

L

A

D

D

E

E

V

V

M

I

C

C

G

A

M

F

G

G

R

R

T

L

G

G

T

T

L

M

F

F

A
x
G

C

C

E

D

Q

K

D

Y

G

N

I

I

S

K

P

P

D

D

I

L

V

V

T

T

I

L

A

A

K

K

G

A

L

L

G

S

A

S

G

A

Y

Y

A

M

P

P

I

I

G

G

A

A

M

I

L

L

A

M

S

S

G

Q

R

E

I

V

Y

A

D

D

A

V

I

I

A

N

Q

S

G

H

S

S

-

S

-

K

-

L

G

G

S

V

F

F

Q

S

H

H

G

G

H

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T

Y

Y

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G

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H

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P

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V

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L

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A

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I

I

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K

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L

L

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A

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C98 (≠ V41) mutation to Y: In her1-1; loss of E2-hexenal responses.
G103 (≠ H46) mutation to N: In her1-2; loss of E2-hexenal responses.
G271 (≠ A213) mutation to E: In pop2-6; Loss of activity and complete sterility.
G312 (≠ A254) mutation to D: In pop2-7; loss of activity.

5kr3A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
33% identity, 94% coverage: 19:437/444 of query aligns to 39:452/458 of 5kr3A

query
sites
5kr3A

G

G

A

K

G

G

C

I

Y

R

I

I

I

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D

D

A

H

D

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G

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K

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Y

Y

L

Y

L

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G
x
S

G
|
G

S
|
S

E

D

S

A

V
x
N

E

E

A

T

A

Q

L

I

K

K

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Y

F

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N

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K

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Y
|
Y

H
|
H

G
|
G

N

S

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G

L

I

G

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A

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L

G

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|
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I

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binding pyridoxal-5'-phosphate: S120 (≠ G98), G121 (= G99), S122 (= S100), N125 (≠ V103), Y154 (= Y132), H155 (= H133), G156 (= G134), E225 (= E206), D258 (= D240), V260 (= V242), K287 (= K269), W323 (≠ H302), T324 (= T303)

5ghgB Transaminase w58l with smba
34% identity, 98% coverage: 8:441/444 of query aligns to 21:430/433 of 5ghgB

query
sites
5ghgB

R

R

S

L

A

E

A

A

Q

E

M

G

P

P

T

L

A

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V

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L

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G
x
S

G
|
G

S
|
S

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E

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A

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x
N

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D

A

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A

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L

V

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K

L

L

A

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G

Y
|
Y

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|
H

G
|
G

N

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T

T

L

I

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A

A

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A

A

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