SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3608131 FitnessBrowser__Dino:3608131 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0AAH0 Phosphate import ATP-binding protein PstB; ABC phosphate transporter; Phosphate-transporting ATPase; EC 7.3.2.1 from Escherichia coli (strain K12) (see paper)
58% identity, 96% coverage: 12:264/264 of query aligns to 6:257/257 of P0AAH0

query
sites
P0AAH0

T

T

E

A

Q

P

T

S

K

K

I

I

A

Q

A

V

K

R

N

N

V

L

Q

N

V

F

Y

Y

G

G

T

K

T

F

H

H

A

A

I

L

K

K

D

N

V

I

N

N

V

L

D

D

I

I

L

A

D

K

K

N

T

Q

V

V

T

T

A

A

F

F

I

I

G

G

P

P

S

S

G

G

C

C

G
|
G

K
|
K

S

S

T

T

F

L

L

L

R

R

C

T

L

F

N

N

R

K

M

M

N

F

D

E

T

L

I

Y

D

P

T

E

A

Q

R

R

I

A

E

E

G

G

D

E

I

I

R

L

I

L

D

D

G

G

E

D

D

N

I

I

Y

L

D

T

R

N

R

S

V

Q

D

D

P

I

V

A

Q

L

L

L

R

R

A

A

K

K

V

V

G

G

M

M

V

V

F

F

Q

Q

K

K

P

P

N

T

P

P

F

F

P

P

K

M

S

S

I

I

Y

Y

D

D

N

N

V

I

A

A

Y

F

G

G

P

V

R

R

I

L

H

F

G

E

L

-

A

K

R

L

T

S

R

R

A

A

E

D

L

M

D

D

E

E

I

R

V

V

E

Q

K

W

S

A

L

L

R

T

R

K

A

A

L

L

W

W

D

N

E

E

A

T

K

K

D

D

R

K

L

L

D

H

Q

Q

P

S

G

G

T

Y

G

S

L

L

S

S

G

G

Q

Q

R

R

L

L

C

C

I

I

A

A

R

R

A

G

V

I

A

A

T

I

S

R

P

P

E

E

V

V

L

L

M

L

D

D

E

E

P

P

C

C

S

S

A

A

L

L

D

D

P

P

I

I

A

S

T

T

A

G

Q

R

V

I

E

E

L

L

I

I

D

T

E

E

L

L

R

K

E

Q

R

D

Y

Y

S

T

V

V

I

I

V

V

T

T

H

H

S

N

M

M

Q

Q

A

A

R

R

V

C

S

S

Q

D

R

H

T

T

A

A

F

F

F

M

H

Y

L

L

G

G

H

E

L

L

V

I

E

E

Y

F

G

S

E

N

T

T

G

D

H

D

I

L

F

F

T

T

N

K

P

P

E

A

D

K

P

K

R

Q

T

T

E

E

S

D

Y

Y

I

I

T

T

G

G

R

R

I

Y

G

G

G48 (= G54) mutation to I: Loss of phosphate transport.
K49 (= K55) mutation to Q: Loss of phosphate transport.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
37% identity, 92% coverage: 17:259/264 of query aligns to 3:236/241 of 4u00A

query
sites
4u00A

I

I

A

R

A

I

K

R

N

N

V

L

Q

H

V

K

Y

W

Y
x
F

G

G

T

P

T

L

H

H

A
x
V

I

L

K

K

D

G

V

I

N

H

V

L

D

E

I

V

L

A

D

P

K

G

T

E

V

K

T

L

A

V

F

I

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

T

L

I

N

N

R

R

M

L

N

E

D

D

T

F

I

Q

D

-

T

-

A

-

R

-

I

-

E

E

G

G

D

E

I

V

R

V

I

V

D

D

G

G

E

L

D

S

I

V

Y

K

D

D

R

D

R

R

V

A

D

L

P

R

V

-

Q

E

L

I

R

R

A

R

K

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

Q

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

Y

L

D

E

N

N

V

V

A

T

Y

L

G

A

P

P

-

M

R

R

I

V

H

R

G

R

L

W

A

P

R

R

T

E

R

K

A

A

E

E

L

K

D

K

E

A

I

L

V

-

E

-

K

E

S

L

L

L

R

E

R

R

A

V

A

G

L

I

W

L

D

D

E

Q

A

A

K

R

D

K

R

Y

L

P

D

A

Q

Q

P

-

G

-

T

-

G

-

L

L

S

S

G

G

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

V

L

A

A

T

M

S

E

P

P

E

K

V

I

L

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

Q

E

V

V

E

L

E

D

L

V

I

M

D

R

E

D

L

L

R

A

E

Q

-

G

R

G

Y

M

S

T

V

M

V

V

I

V

V

V

T

T

H

H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

A

Q

D

R

R

T

V

A

V

F

F

F

M

H

D

L

G

G

G

H

Q

L

I

V

V

E

E

Y

E

G

G

E

R

T

P

G

E

H

E

I

I

F

F

T

T

N

R

P

P

E

K

D

E

P

E

R

R

T

T

E

R

S

S

Y

F

I

L

binding adenosine-5'-diphosphate: F12 (≠ Y26), V17 (≠ A31), S37 (= S51), G38 (= G52), S39 (≠ C53), G40 (= G54), K41 (= K55), S42 (= S56), T43 (= T57)

3c4jA Abc protein artp in complex with atp-gamma-s
38% identity, 89% coverage: 26:260/264 of query aligns to 13:239/242 of 3c4jA

query
sites
3c4jA

Y
x
F

G

G

T

S

T

L

H

E

A
x
V

I

L

K

K

D

G

V

I

N

N

V

V

D

H

I

I

L

R

D

E

K

G

T

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

C

C

L

L

N

N

R

L

M

L

N

E

D

D

T

-

I

F

D

D

T

-

A

-

R

-

I

-

E

E

G

G

D

E

I

I

R

I

I

I

D

D

G

G

E

I

D

N

I

L

Y

K

D

A

R

K

R

D

V

T

D

N

P

L

V

N

Q

K

L

V

R

R

A

E

K

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

Y

L

D

N

N

N

V

I

A

T

Y

L

G

A

P

P

-

M

R

K

I

V

H

R

G

K

L

W

A

P

R

R

T

E

R

K

A

A

E

E

L

A

D

K

E

A

I

M

V

-

E

-

K

E

S

L

L

L

R

D

R

K

A

V

A

G

L

L

W

K

D

D

E

K

A

A

K

H

D

A

R

Y

L

P

D

D

Q

S

P

-

G

-

T

-

G

-

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

V

L

A

A

T

M

S

E

P

P

E

K

V

I

L

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

Q

E

V

V

E

L

E

S

L

V

I

M

D

K

E

Q

L

L

-

A

R

N

E

E

R

G

Y

M

S

T

V

M

V

V

I

V

V

V

T

T

H

H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

L

F

F

F

M

H

D

L

G

G

G

H

Y

L

I

V

I

E

E

Y

E

G

G

E

K

T

P

G

E

H

D

I

L

F

F

T

D

N

R

P

P

E

Q

D

H

P

E

R

R

T

T

E

K

S

A

Y

F

I

L

T

S

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y26), V18 (≠ A31), S38 (= S51), G39 (= G52), S40 (≠ C53), G41 (= G54), K42 (= K55), S43 (= S56), T44 (= T57)

3c41J Abc protein artp in complex with amp-pnp/mg2+
38% identity, 89% coverage: 26:260/264 of query aligns to 13:239/242 of 3c41J

query
sites
3c41J

Y
x
F

G

G

T

S

T

L

H

E

A
x
V

I

L

K

K

D

G

V

I

N

N

V

V

D

H

I

I

L

R

D

E

K

G

T

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

C

C

L

L

N

N

R

L

M

L

N

E

D

D

T

-

I

F

D

D

T

-

A

-

R

-

I

-

E

E

G

G

D

E

I

I

R

I

I

I

D

D

G

G

E

I

D

N

I

L

Y

K

D

A

R

K

R

D

V

T

D

N

P

L

V

N

Q

K

L

V

R

R

A

E

K

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

Y

L

D

N

N

N

V

I

A

T

Y

L

G

A

P

P

-

M

R

K

I

V

H

R

G

K

L

W

A

P

R

R

T

E

R

K

A

A

E

E

L

A

D

K

E

A

I

M

V

-

E

-

K

E

S

L

L

L

R

D

R

K

A

V

A

G

L

L

W

K

D

D

E

K

A

A

K

H

D

A

R

Y

L

P

D

D

Q

S

P

-

G

-

T

-

G

-

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

V

L

A

A

T

M

S

E

P

P

E

K

V

I

L

M

L

L

M

F

D
|
D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

Q

E

V

V

E

L

E

S

L

V

I

M

D

K

E

Q

L

L

-

A

R

N

E

E

R

G

Y

M

S

T

V

M

V

V

I

V

V

V

T

T

H

H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

L

F

F

F

M

H

D

L

G

G

G

H

Y

L

I

V

I

E

E

Y

E

G

G

E

K

T

P

G

E

H

D

I

L

F

F

T

D

N

R

P

P

E

Q

D

H

P

E

R

R

T

T

E

K

S

A

Y

F

I

L

T

S

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y26), V18 (≠ A31), S38 (= S51), G39 (= G52), G41 (= G54), K42 (= K55), S43 (= S56), T44 (= T57)
binding magnesium ion: S43 (= S56), D163 (= D185)

2olkA Abc protein artp in complex with adp-beta-s
38% identity, 89% coverage: 26:260/264 of query aligns to 13:239/242 of 2olkA

query
sites
2olkA

Y
x
F

G

G

T

S

T

L

H

E

A
x
V

I

L

K

K

D

G

V

I

N

N

V

V

D

H

I

I

L

R

D

E

K

G

T

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

C

C

L

L

N

N

R

L

M

L

N

E

D

D

T

-

I

F

D

D

T

-

A

-

R

-

I

-

E

E

G

G

D

E

I

I

R

I

I

I

D

D

G

G

E

I

D

N

I

L

Y

K

D

A

R

K

R

D

V

T

D

N

P

L

V

N

Q

K

L

V

R

R

A

E

K

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

Y

L

D

N

N

N

V

I

A

T

Y

L

G

A

P

P

-

M

R

K

I

V

H

R

G

K

L

W

A

P

R

R

T

E

R

K

A

A

E

E

L

A

D

K

E

A

I

M

V

-

E

-

K

E

S

L

L

L

R

D

R

K

A

V

A

G

L

L

W

K

D

D

E

K

A

A

K

H

D

A

R

Y

L

P

D

D

Q

S

P

-

G

-

T

-

G

-

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

V

L

A

A

T

M

S

E

P

P

E

K

V

I

L

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

Q

E

V

V

E

L

E

S

L

V

I

M

D

K

E

Q

L

L

-

A

R

N

E

E

R

G

Y

M

S

T

V

M

V

V

I

V

V

V

T

T

H

H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

L

F

F

F

M

H

D

L

G

G

G

H

Y

L

I

V

I

E

E

Y

E

G

G

E

K

T

P

G

E

H

D

I

L

F

F

T

D

N

R

P

P

E

Q

D

H

P

E

R

R

T

T

E

K

S

A

Y

F

I

L

T

S

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y26), V18 (≠ A31), S38 (= S51), G39 (= G52), S40 (≠ C53), G41 (= G54), K42 (= K55), S43 (= S56), T44 (= T57)

2oljA Abc protein artp in complex with adp/mg2+
38% identity, 89% coverage: 26:260/264 of query aligns to 13:239/242 of 2oljA

query
sites
2oljA

Y
x
F

G

G

T

S

T

L

H

E

A
x
V

I

L

K

K

D

G

V

I

N

N

V

V

D

H

I

I

L

R

D

E

K

G

T

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

C

C

L

L

N

N

R

L

M

L

N

E

D

D

T

-

I

F

D

D

T

-

A

-

R

-

I

-

E

E

G

G

D

E

I

I

R

I

I

I

D

D

G

G

E

I

D

N

I

L

Y

K

D

A

R

K

R

D

V

T

D

N

P

L

V

N

Q

K

L

V

R

R

A

E

K

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

Y

L

D

N

N

N

V

I

A

T

Y

L

G

A

P

P

-

M

R

K

I

V

H

R

G

K

L

W

A

P

R

R

T

E

R

K

A

A

E

E

L

A

D

K

E

A

I

M

V

-

E

-

K

E

S

L

L

L

R

D

R

K

A

V

A

G

L

L

W

K

D

D

E

K

A

A

K

H

D

A

R

Y

L

P

D

D

Q

S

P

-

G

-

T

-

G

-

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

V

L

A

A

T

M

S

E

P

P

E

K

V

I

L

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

Q

E

V

V

E

L

E

S

L

V

I

M

D

K

E

Q

L

L

-

A

R

N

E

E

R

G

Y

M

S

T

V

M

V

V

I

V

V

V

T

T

H

H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

L

F

F

F

M

H

D

L

G

G

G

H

Y

L

I

V

I

E

E

Y

E

G

G

E

K

T

P

G

E

H

D

I

L

F

F

T

D

N

R

P

P

E

Q

D

H

P

E

R

R

T

T

E

K

S

A

Y

F

I

L

T

S

binding adenosine-5'-diphosphate: F13 (≠ Y26), V18 (≠ A31), S38 (= S51), G39 (= G52), S40 (≠ C53), G41 (= G54), K42 (= K55), S43 (= S56), T44 (= T57)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
37% identity, 92% coverage: 17:260/264 of query aligns to 2:237/240 of 4ymuJ

query
sites
4ymuJ

I

I

A

F

A

V

K

N

N

D

V

V

Q

Y

V

K

Y

N

Y
x
F

G

G

T

S

T

L

H

E

A
x
V

I

L

K

K

D

G

V

V

N

T

V

L

D

K

I

V

L

N

D

K

K

G

T

E

V

V

T

V

A

V

F

I

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

C

C

L

I

N

N

R

L

M

L

N

E

D

E

T

P

I

T

D

-

T

-

A

-

R

-

I

-

E

K

G

G

D

E

I

V

R

F

I

I

D

D

G

G

E

V

D

K

I

I

Y

N

D

N

R

G

R

K

V

V

D

N

P

I

V

N

Q

K

L

V

R

R

A

Q

K

K

V

V

G

G

M

M

V

V

F

F

Q

Q

K

H

P

F

N

N

P

L

F

F

P

P

K

H

-

L

S

T

I

A

Y

I

D

E

N

N

V

I

A

T

Y

L

G

A

P

P

-

V

R

K

I

V

H

K

G

K

L

M

A

N

R

K

T

K

R

E

A

A

E

E

L

-

D

-

E

E

I

L

V

A

E

V

K

D

S

L

L

L

R

A

R

K

A

V

A

G

L

L

W

L

D

D

E

K

A

K

K

-

D

-

R

-

L

-

D

D

Q

Q

P

Y

G

P

T

I

G

K

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

L

C

A

I

I

A

A

R

R

A

A

V

L

A

A

T

M

S

Q

P

P

E

E

V

V

L

M

L

L

M

F

D

D

E
|
E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

K

Q

E

V

V

E

L

E

N

L

V

I

M

D

K

E

Q

L

L

-

A

R

N

E

E

R

G

Y

M

S

T

V

M

V

V

I

V

V

V

T

T

H
|
H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

I

F

F

F

M

H

D

L

D

G

G

H

V

L

I

V

V

E

E

Y

E

G

G

E

T

T

P

G

E

H

E

I

I

F

F

T

Y

N

R

P

A

E

K

D

N

P

E

R

R

T

T

E

R

S

E

Y

F

I

L

T

S

binding adenosine-5'-triphosphate: F11 (≠ Y26), V16 (≠ A31), S36 (= S51), G37 (= G52), S38 (≠ C53), G39 (= G54), K40 (= K55), S41 (= S56), T42 (= T57), E162 (= E186), H194 (= H217)
binding magnesium ion: S41 (= S56), E162 (= E186)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
34% identity, 88% coverage: 27:259/264 of query aligns to 37:263/382 of 7ahhC

query
sites
7ahhC

G

G

T

A

T
|
T

H
x
V

A
x
G

I

V

K

Y

D

D

V

T

N

N

V

F

D

E

I

I

L

N

D

E

K

G

T

E

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S

S

G
|
G

C

S

G
|
G

K
|
K

S

S

T

T

F

L

L

L

R

R

C

L

L

L

N

N

R

R

M

L

N

I

D

E

T

P

I

T

D

-

T

-

A

-

R

-

I

-

E

S

G

G

D

K

I

I

R

F

I

I

D

D

G

N

E

Q

D

D

I

V

Y

A

D

T

-

L

R

N

R

K

V

E

D

D

P

L

V

L

Q

Q

L

V

R

R

A

R

K

K

-

T

V

M

G

S

M

M

V

V

F

F

Q

Q

K

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

Y

L

D

E

N

N

V

T

A

E

Y

Y

G

G

P

L

R

E

I

V

H

Q

G

N

L

V

A

P

R

K

T

-

R

-

A

E

E

E

L

R

D

R

E

K

I

R

V

A

E

E

K

K

S

A

L

L

R

D

R

N

A

A

A

N

L

L

W

L

D

D

E

F

A

K

K

-

D

-

R

-

L

-

D

D

Q

Q

P

Y

G

P

T

K

G

Q

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

V

L

A

A

T

N

S

D

P

P

E

E

V

I

L

L

M

M

D
|
D

E
|
E

P

A

C

F

S

S

A

A

L

L

D

D

P

P

I

L

A

I

T

R

A

R

Q

E

V

M

E

Q

E

D

L

E

I

L

D

L

E

E

L

L

R

Q

E

A

R

K

Y

F

-

Q

-

K

S

T

V

I

I

F

V

V

T

S

H

H

S

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

Q

D

R

R

T

I

A

A

F

I

F

M

H

K

L

D

G

G

H

K

L

I

V

M

E

Q

Y

I

G

G

E

T

T

G

G

E

H

E

I

I

F

L

T

T

N

N

P

P

E

A

D

N

P

D

R

Y

T

V

E

K

S

T

Y

F

I

V

binding phosphoaminophosphonic acid-adenylate ester: T39 (= T29), V40 (≠ H30), G41 (≠ A31), G62 (= G52), G64 (= G54), K65 (= K55), D187 (= D185), E188 (= E186)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
34% identity, 88% coverage: 27:259/264 of query aligns to 37:263/382 of 7aheC

query
sites
7aheC

G

G

T

A

T

T

H

V

A

G

I

V

K

Y

D

D

V

T

N

N

V

F

D

E

I

I

L

N

D

E

K

G

T

E

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S

S

G

G

C

S

G

G

K

K

S

S

T

T

F

L

L

L

R

R

C

L

L

L

N

N

R

R

M

L

N

I

D

E

T

P

I

T

D

-

T

-

A

-

R

-

I

-

E

S

G

G

D

K

I

I

R

F

I

I

D

D

G

N

E

Q

D

D

I

V

Y

A

D

T

-

L

R

N

R

K

V

E

D

D

P

L

V

L

Q

Q

L

V

R

R

A

R

K

K

-

T

V

M

G

S

M

M

V

V

F

F

Q

Q

K

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

Y

L

D

E

N

N

V

T

A

E

Y

Y

G

G

P

L

R

E

I

V

H

Q

G

N

L

V

A

P

R

K

T

-

R

-

A

E

E

E

L

R

D

R

E

K

I

R

V

A

E

E

K

K

S

A

L

L

R

D

R

N

A

A

A

N

L

L

W

L

D

D

E

F

A

K

K

-

D

-

R

-

L

-

D

D

Q

Q

P

Y

G

P

T

K

G

Q

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

V

L

A

A

T

N

S

D

P

P

E

E

V

I

L

L

M

M

D

D

E

E

P

A

C

F

S

S

A

A

L

L

D

D

P

P

I

L

A

I

T

R

A

R

Q

E

V

M

E

Q

E

D

L

E

I

L

D

L

E

E

L

L

R

Q

E

A

R

K

Y

F

-

Q

-

K

S

T

V

I

I

F

V

V

T

S

H

H

S

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

Q

D

R

R

T

I

A

A

F

I

F

M

H

K

L

D

G

G

H

K

L

I

V

M

E

Q

Y

I

G

G

E

T

T

G

G

E

H

E

I

I

F

L

T

T

N

N

P

P

E

A

D

N

P

D

R

Y

T

V

E

K

S

T

Y

F

I

V

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

7ahdC Opua (e190q) occluded (see paper)
35% identity, 85% coverage: 27:250/264 of query aligns to 37:254/260 of 7ahdC

query
sites
7ahdC

G

G

T

A

T
|
T

H

V

A

G

I

V

K

Y

D

D

V

T

N

N

V

F

D

E

I

I

L

N

D

E

K

G

T

E

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

C

L

L

L

N

N

R

R

M

L

N

I

D

E

T

P

I

T

D

S

T

-

A

-

R

-

I

-

E

-

G

G

D

K

I

I

R

F

I

I

D

D

G

N

E

Q

D

D

I

V

Y

A

D

T

-

L

R

N

R

K

V

E

D

D

P

L

V

L

Q

Q

L

V

R

R

A

R

K

K

-

T

V

M

G

S

M

M

V

V

F

F

Q
|
Q

K

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

Y

L

D

E

N

N

V

T

A

E

Y

Y

G

G

P

L

R

E

I

V

H

Q

G

N

L

V

A

P

R

K

T

-

R

-

A

E

E

E

L

R

D

R

E

K

I

R

V

A

E

E

K

K

S

A

L

L

R

D

R

N

A

A

A

N

L

L

W

L

D

D

E

F

A

K

K

-

D

-

R

-

L

-

D

D

Q

Q

P

Y

G

P

T
x
K

G
x
Q

L

L

S
|
S

G

G

G
|
G

Q
x
M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

V

L

A

A

T

N

S

D

P

P

E

E

V

I

L

L

M

M

D

D

E
x
Q

P

A

C

F

S

S

A

A

L

L

D

D

P

P

I

L

A

I

T

R

A

R

Q

E

V

M

E

Q

E

D

L

E

I

L

D

L

E

E

L

L

R

Q

E

A

R

K

Y

F

-

Q

-

K

S

T

V

I

I

F

V

V

T

S

H
|
H

S

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

Q

D

R

R

T

I

A

A

F

I

F

M

H

K

L

D

G

G

H

K

L

I

V

M

E

Q

Y

I

G

G

E

T

T

G

G

E

H

E

I

I

F

L

T

T

N

N

P

P

binding adenosine-5'-triphosphate: T39 (= T29), S61 (= S51), G62 (= G52), G64 (= G54), K65 (= K55), S66 (= S56), T67 (= T57), Q111 (= Q104), K161 (≠ T159), Q162 (≠ G160), S164 (= S162), G166 (= G164), M167 (≠ Q165), Q188 (≠ E186), H221 (= H217)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

7y48B Cryo-em structure of biliverdin-bound mitochondrial abc transporter abcb10 from biortus
34% identity, 90% coverage: 14:250/264 of query aligns to 335:565/567 of 7y48B

query
sites
7y48B

Q

Q

T

G

K

A

I

L

A

E

A

F

K

K

N

N

V

V

Q

H

V

F

Y

A

Y

Y

G

P

T

A

T

R

H

P

A

E

I

V

-

P

-

I

-

F

K

Q

D

D

V

F

N

S

V

L

D

S

I

I

L

P

D

S

K

G

T

S

V

V

T

T

A

A

F

L

I

V

G

G

P

P

S

S

G

G

C

S

G

G

K

K

S

S

T

T

F

V

L

L

R

S

C

L

L

L

N

L

R

R

M

L

N

Y

D

D

T

P

I

A

D

S

T

-

A

-

R

-

I

-

E

-

G

G

D

T

I

I

R

S

I

L

D

D

G

G

E

H

D

D

I

I

Y

-

D

-

R

R

R

Q

V

L

D

N

P

P

V

V

Q

W

L

L

R

R

A

S

K

K

V

I

G

G

M

T

V

V

F

S

Q

Q

K

E

P

P

N

I

P

L

F

F

P

S

K

C

S

S

I

I

Y

A

D

E

N

N

V

I

A

A

Y

Y

G

G

-

A

-

D

P

P

R

S

I

-

H

-

G

-

L

-

A

S

R

V

T

T

R

A

A

E

E

E

L

I

D

Q

E

R

I

V

V

A

E

E

K

V

S

A

L

N

R

A

R

V

A

A

A

F

L

I

W

R

D

N

E

F

A

P

K

Q

D

G

R

F

L

N

D

T

Q

V

P

V

G

G

T

V

G

L

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

V

L

A

L

T

K

S

N

P

P

E

K

V

I

L

L

M

L

D

D

E

Q

P

A

C

T

S

S

A

A

L

L

D

D

P

A

I

E

A

N

T

E

A

Y

Q

L

V

V

E

Q

E

E

L

A

I

L

D

D

E

R

L

L

R

M

E

D

R

G

Y

R

S

T

V

V

V

L

I

V

V

I

T

A

H

H

S

R

M

L

Q

-

Q

S

A

T

A

I

R

K

V

N

S

A

Q

N

R

M

T

V

A

A

F

V

F

L

H

D

L

Q

G

G

H

K

L

I

V

T

E

E

Y

Y

G

G

E

K

T

H

G

E

H

E

I

L

F

L

T

S

N

K

P

P

Sites not aligning to the query:

binding Biliverdine IX Alpha: 171, 248, 252, 255

4ayxA Structure of the human mitochondrial abc transporter, abcb10 (rod form b) (see paper)
34% identity, 90% coverage: 14:250/264 of query aligns to 336:569/571 of 4ayxA

query
sites
4ayxA

Q

Q

T

G

K

A

I

L

A

E

A

F

K

K

N

N

V

V

Q

H

V

F

Y

A

Y
|
Y

G

P

T

A

T

R

H

P

A

E

I

V

-

P

-

I

-

F

K

Q

D

D

V

F

N

S

V

L

D

S

I

I

L

P

D

S

K

G

T

S

V

V

T

T

A

A

F

L

I

V

G

G

P

P

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

V

L

L

R

S

C

L

L

L

N

L

R

R

M

L

N

Y

D

D

T

P

I

A

D

S

T

-

A

-

R

-

I

-

E

-

G

G

D

T

I

I

R

S

I

L

D

D

G

G

E

H

D

D

I

I

Y

-

D

-

R

R

R

Q

V

L

D

N

P

P

V

V

Q

W

L

L

R

R

A

S

K

K

V

I

G

G

M

T

V

V

F

S

Q
|
Q

K

E

P

P

N

I

P

L

F

F

P

S

K

C

S

S

I

I

Y

A

D

E

N

N

V

I

A

A

Y

Y

G

G

-

A

-

D

P

P

R

S

I

-

H

-

G

-

L

-

A

S

R

V

T

T

R

A

A

E

E

E

L

I

D

Q

E

R

I

V

V

A

E

E

K

V

S

A

L

N

R

A

R

V

A

A

A

F

L

I

W

R

D

N

E

F

A

P

K

Q

D

G

R

F

-

N

-

T

-

V

L

V

D

G

Q

E

P

K

G

G

T

V

G

L

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

V

L

A

L

T

K

S

N

P

P

E

K

V

I

L

L

M

L

D

D

E

E

P

A

C

T

S

S

A

A

L

L

D

D

P

A

I

E

A

N

T

E

A

Y

Q

L

V

V

E

Q

E

E

L

A

I

L

D

D

E

R

L

L

R

M

E

D

R

G

Y

R

S

T

V

V

V

L

I

V

V

I

T

A

H

H

S

R

M

L

Q

-

Q

S

A

T

A

I

R

K

V

N

S

A

Q

N

R

M

T

V

A

A

F

V

F

L

H

D

L

Q

G

G

H

K

L

I

V

T

E

E

Y

Y

G

G

E

K

T

H

G

E

H

E

I

L

F

L

T

S

N

K

P

P

binding phosphomethylphosphonic acid adenylate ester: Y348 (= Y26), S376 (= S51), G377 (= G52), G379 (= G54), K380 (= K55), S381 (= S56), T382 (= T57)
binding magnesium ion: S381 (= S56), Q422 (= Q104)

Sites not aligning to the query:

binding glycine: 17, 81

P02915 Histidine/lysine/arginine/ornithine transport ATP-binding protein HisP; EC 7.4.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
35% identity, 96% coverage: 11:263/264 of query aligns to 1:257/258 of P02915

query
sites
P02915

V

M

T

M

E

S

Q

E

T

N

K

K

I

L

A

H

A

V

K

I

N

D

V

L

Q

H

V

K

Y

R

Y

Y

G

G

T

G

T

H

H

E

A

V

I

L

K

K

D

G

V

V

N

S

V

L

D

Q

I

A

L

R

D

A

K

G

T

D

V

V

T

I

A

S

F

I

I

I

G

G

P

S

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

C

C

L

I

N

N

R

F

M

L

N

E

D

K

T

P

I

S

D

-

T

-

A

-

R

-

I

-

E

E

G

G

D

A

I

I

R

I

I

V

D

N

G

G

E

Q

D

N

I

I

Y

N

D

L

R

V

R

R

-

D

-

K

-

D

-

G

-

Q

-

L

-

K

-

V

V

A

D

D

P

K

V

N

Q

Q

L

L

R

R

-

L

-

R

A

T

K

R

V

L

G

T

M

M

V

V

F

F

Q

Q

K

H

P

F

N

N

P

L

F

W

P

S

K

H

-

M

S

T

I

V

Y

L

D

E

N

N

V

V

A

M

Y

E

G

A

P

P

-

I

R

Q

I

V

H

L

G

G

L

L

A

S

R

K

T

H

R

D

A

A

E

-

L

-

D

-

E

-

I

-

V

-

E

-

K

-

S

-

L

-

R

R

R

E

A

R

A

A

L

L

W

K

D

Y

E

L

A

A

K

K

D

V

R

G

L

I

D

D

Q

E

P

R

G

A

T

Q

G

G

-

K

-

Y

-

P

-

V

-

H

L

L

S

S

G

G

Q

Q

R

R

L

V

C

S

I

I

A

A

R

R

A

A

V

L

A

A

T

M

S

E

P

P

E

D

V

V

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

L

T

V

A

G

Q

E

V

V

E

L

E

R

L

I

I

M

D

Q

E

Q

L

L

R

A

E

E

R

E

-

G

Y

K

S

T

V

M

V

V

I

V

V

V

T

T

H

H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

H

V

V

S

S

Q

S

R

H

T

V

A

I

F

F

F

L

H

H

L

Q

G

G

H

K

L

I

V

E

E

E

Y

E

G

G

E

D

T

P

G

E

H

Q

I

V

F

F

T

G

N

N

P

P

E

Q

D

S

P

P

R

R

T

L

E

Q

S

Q

Y

F

I

L

T

K

G

G

R

S

I

L

S41 (= S51) binding
G42 (= G52) binding
G44 (= G54) binding
K45 (= K55) binding
S46 (= S56) binding
T47 (= T57) binding

Q9NRK6 ATP-binding cassette sub-family B member 10, mitochondrial; ABC-mitochondrial erythroid protein; ABC-me protein; ATP-binding cassette transporter 10; ABC transporter 10 protein; Mitochondrial ATP-binding cassette 2; M-ABC2 from Homo sapiens (Human) (see 5 papers)
34% identity, 90% coverage: 14:251/264 of query aligns to 489:723/738 of Q9NRK6

query
sites
Q9NRK6

Q

Q

T

G

K

A

I

L

A

E

A

F

K

K

N

N

V

V

Q

H

V

F

Y

A

Y

Y

G

P

T

A

T

R

H

P

A

E

I

V

-

P

-

I

-

F

K

Q

D

D

V

F

N

S

V

L

D

S

I

I

L

P

D

S

K

G

T

S

V

V

T

T

A

A

F

L

I

V

G

G

P

P

S

S

G

G

C

S

G

G

K
|
K

S

S

T

T

F

V

L

L

R

S

C

L

L

L

N

L

R

R

M

L

N

Y

D
|
D

T

P

I

A

D

S

T

-

A

-

R

-

I

-

E

-

G

G

D

T

I

I

R

S

I

L

D

D

G

G

E

H

D

D

I

I

Y

-

D

-

R

R

R

Q

V

L

D

N

P

P

V

V

Q

W

L

L

R

R

A

S

K

K

V

I

G

G

M

T

V

V

F

S

Q

Q

K

E

P

P

N

I

P

L

F

F

P

S

K
x
C

S

S

I

I

Y

A

D

E

N

N

V

I

A

A

Y

Y

G

G

-

A

-

D

P

P

R

S

I

-

H

-

G

-

L

-

A

S

R

V

T

T

R

A

A

E

E

E

L

I

D

Q

E

R

I

V

V

A

E

E

K

V

S

A

L

N

R

A

R

V

A

A

A

F

L

I

W

R

D

N

E

F

A

P

K

Q

D

G

R

F

-

N

-

T

-

V

L

V

D

G

Q

E

P

K

G

G

T

V

G

L

L

L

S
|
S

G

G

Q
|
Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

V

L

A

L

T

K

S

N

P

P

E

K

V

I

L

L

M

L

D
|
D

E
|
E

P

A

C

T

S

S

A

A

L

L

D

D

P

A

I

E

A

N

T

E

A

Y

Q

L

V

V

E

Q

E

E

L

A

I

L

D

D

E

R

L

L

R

M

E

D

R

G

Y

R

S

T

V

V

V

L

I

V

V

I

T

A

H

H

S

R

M

L

Q

S

Q

T

A

-

A

I

R

K

V

N

S

A

Q

N

R

M

T

V

A

A

F

V

F

L

H

D

L

Q

G

G

H

K

L

I

V

T

E

E

Y

Y

G

G

E

K

T

H

G

E

H

E

I

L

F

L

T

S

N

K

P

P

E

N

K533 (= K55) mutation to E: Increases hemoglobin biosynthetic process.
D545 (= D67) to N: in dbSNP:rs35698797
C582 (≠ K111) mutation to G: Does not affect ATPase activity; when associated with S-215 and L-224. Activated by Zn (II) mesoporphyrin; when associated with S-215 and L-224.
S635 (= S162) mutation to R: Does not rescue hemoglobin and heme biosynthetic process.
Q638 (= Q165) mutation to H: Does not rescue hemoglobin and heme biosynthetic process.
D658 (= D185) mutation to A: Does not rescue hemoglobin and heme biosynthetic process.
E659 (= E186) mutation to A: Does not rescue hemoglobin and heme biosynthetic process.

Sites not aligning to the query:

150 A → S: in dbSNP:rs4148756
215 C→S: Does not affect ATPase activity; when associated with L-224 and G-582. Activated by Zn (II) mesoporphyrin; when associated with L-224 and G-582.
224 C→L: Does not affect ATPase activity; when associated withS-215 and G-582. Activated by Zn (II) mesoporphyrin; when associated with S-215 and G-582.
471 R → T: in a breast cancer sample; somatic mutation

1b0uA Atp-binding subunit of the histidine permease from salmonella typhimurium (see paper)
35% identity, 94% coverage: 15:263/264 of query aligns to 1:253/258 of 1b0uA

query
sites
1b0uA

T

N

K

K

I

L

A

H

A

V

K

I

N

D

V

L

Q

H

V

K

Y

R

Y
|
Y

G

G

T

G

T
x
H

H

E

A

V

I

L

K

K

D

G

V

V

N

S

V

L

D

Q

I

A

L

R

D

A

K

G

T

D

V

V

T

I

A

S

F

I

I

I

G

G

P

S

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

C

C

L

I

N

N

R

F

M

L

N

E

D

K

T

P

I

S

D

-

T

-

A

-

R

-

I

-

E

E

G

G

D

A

I

I

R

I

I

V

D

N

G

G

E

Q

D

N

I

I

Y

N

D

L

R

V

R

R

-

D

-

K

-

D

-

G

-

Q

-

L

-

K

-

V

V

A

D

D

P

K

V

N

Q

Q

L

L

R

R

-

L

-

R

A

T

K

R

V

L

G

T

M

M

V

V

F

F

Q

Q

K

H

P

F

N

N

P

L

F

W

P

S

K

H

-

M

S

T

I

V

Y

L

D

E

N

N

V

V

A

M

Y

E

G

A

P

P

-

I

R

Q

I

V

H

L

G

G

L

L

A

S

R

K

T

H

R

D

A

A

E

-

L

-

D

-

E

-

I

-

V

-

E

-

K

-

S

-

L

-

R

R

R

E

A

R

A

A

L

L

W

K

D

Y

E

L

A

A

K

K

D

V

R

G

L

I

D

D

Q

E

P

R

G

A

T

Q

G

G

-

K

-

Y

-

P

-

V

-

H

L

L

S

S

G

G

Q

Q

R

R

L

V

C

S

I

I

A

A

R

R

A

A

V

L

A

A

T

M

S

E

P

P

E

D

V

V

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

L

T

V

A

G

Q

E

V

V

E

L

E

R

L

I

I

M

D

Q

E

Q

L

L

R

A

E

E

R

E

-

G

Y

K

S

T

V

M

V

V

I

V

V

V

T

T

H

H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

H

V

V

S

S

Q

S

R

H

T

V

A

I

F

F

F

L

H

H

L

Q

G

G

H

K

L

I

V

E

E

E

Y

E

G

G

E

D

T

P

G

E

H

Q

I

V

F

F

T

G

N

N

P

P

E

Q

D

S

P

P

R

R

T

L

E

Q

S

Q

Y

F

I

L

T

K

G

G

R

S

I

L

binding adenosine-5'-triphosphate: Y12 (= Y26), H15 (≠ T29), S37 (= S51), G38 (= G52), G40 (= G54), K41 (= K55), S42 (= S56), T43 (= T57)

Q9JI39 ATP-binding cassette sub-family B member 10, mitochondrial; ABC-mitochondrial erythroid protein; ABC-me protein; ATP-binding cassette transporter 10; ABC transporter 10 protein from Mus musculus (Mouse) (see 2 papers)
32% identity, 91% coverage: 12:250/264 of query aligns to 452:687/715 of Q9JI39

query
sites
Q9JI39

T

T

E

F

Q

Q

T

G

K

A

I

L

A

E

A

F

K

R

N

N

V

V

Q

H

V

F

Y

T

Y

Y

G

P

T

A

-

R

-

P

-

E

T

V

H

S

A

V

I

F

K

Q

D

D

V

F

N

S

V

L

D

S

I

I

L

P

D

S

K

G

T

S

V

V

T

T

A

A

F

L

I

V

G

G

P

P

S

S

G

G

C

S

G
|
G

K
|
K

S

S

T

T

F

V

L

V

R

S

C

L

L

L

N

L

R

R

M

L

N

Y

D

D

T

P

I

N

D

S

T

-

A

-

R

-

I

-

E

-

G

G

D

T

I

V

R

S

I

L

D

D

G

G

E

H

D

D

I

I

Y

-

D

-

R

R

R

Q

V

L

D

N

P

P

V

V

Q

W

L

L

R

R

A

S

K

K

V

I

G

G

M

T

V

V

F

S

Q

Q

K

E

P

P

N

V

P

L

F

F

P

S

K
x
C

S

S

I

V

Y

A

D

E

N

N

V

I

A

A

Y

Y

G

G

P

A

R

D

I

-

H

-

G

-

L

-

A

-

R

-

T

-

R

-

A

-

E

N

L

L

D

S

E

S

I

V

V

T

E

A

K

Q

S

Q

L

V

R

E

R

R

A

A

L

E

W

V

D

A

E

N

A

A

K

A

D

E

R

F

L

I

-

R

-

S

-

F

-

P

-

Q

-

G

-

F

-

D

-

T

-

V

D

G

Q

E

P

K

G

G

T

I

G

L

L

L

S

S

G

G

G
|
G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

V

L

A

L

T

K

S

N

P

P

E

K

V

I

L

L

M

L

D

D

E
|
E

P

A

C

T

S

S

A

A

L

L

D

D

P

A

I

E

A

N

T

E

A

H

Q

L

V

V

E

Q

E

E

L

A

I

L

D

D

E

R

L

L

R

M

E

E

R

G

Y

R

S

T

V

V

V

L

I

I

V

I

T

A

H

H

S

R

M

L

Q

S

Q

T

A

I

A

K

R

N

V

A

S

N

Q

F

R

V

T

A

A

V

F

L

F

D

H

H

L

-

G

G

H

K

L

I

V
x
C

E

E

Y

H

G

G

E

T

T

H

G

E

H

E

I

L

F

L

T

L

N

K

P

P

G497 (= G54) mutation to A: Decreases ATP binding about 50%.
K498 (= K55) mutation to R: Decreases ATP binding about 50%.
C547 (≠ K111) modified: S-glutathionyl cysteine; mutation to A: Does not affect ABCB10 glutathionylation.
G602 (= G164) mutation to D: Affects ATP hydrolysis but not binding.; mutation to V: Affects ATP hydrolysis but not binding.
E624 (= E186) mutation to Q: Affects ATP hydrolysis but not binding.
C675 (≠ V238) mutation to A: Prevents ABCB10 glutathionylation.

Sites not aligning to the query:

1:82 modified: transit peptide, Mitochondrion

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
30% identity, 88% coverage: 28:259/264 of query aligns to 17:241/343 of P30750

query
sites
P30750

T

T

T

I

H

Q

A

A

I

L

K

N

D

N

V

V

N

S

V

L

D

H

I

V

L

P

D

A

K

G

T

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

C

L

V

N

N

R

L

M

L

N

E

D

R

T

P

I

T

D

-

T

-

A

-

R

-

I

-

E

E

G

G

D

S

I

V

R

L

I

V

D

D

G

G

E

Q

D

E

I

L

Y

T

D

T

-

L

R

S

R

E

V

S

D

E

P

L

V

T

Q

K

L

A

R

R

A

R

K

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

Y

F

D

G

N

N

V

V

A

A

Y

L

G

P

P

L

R

E

I

L

H

D

G

N

L

T

A

P

R

K

T

-

R

-

A

-

E

-

L

-

D

D

E

E

I

V

V

K

E

R

K

R

S

V

L

T

R

E

R

L

A

L

A

S

L

L

W

V

D

G

E

L

A

G

K

-

D

D

R

K

L

H

D

D

Q

S

P

Y

G

P

T

S

G

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

V

L

A

A

T

S

S

N

P

P

E

K

V

V

L

L

M

C

D

D

E
|
E

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

A

R

Q

S

V

I

E

L

E

E

L

L

I

L

D

K

E

D

L

I

R

N

E

R

R

R

-

L

-

G

Y

L

S

T

V

I

V

L

I

L

V

I

T

T

H

H

S

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

Q

D

R

C

T

V

A

A

F

V

F

I

H

S

L

N

G

G

H

E

L

L

V

I

E

E

Y

Q

G

D

E

T

T

V

G

S

H

E

I

V

F

F

T

S

N

H

P

P

E

K

D

T

P

P

R

L

T

A

E

Q

S

K

Y

F

I

I

40:46 (vs. 51:57, 86% identical) binding
E166 (= E186) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

4q9iA P-glycoprotein cocrystallised with qz-ala (see paper)
33% identity, 86% coverage: 14:241/264 of query aligns to 356:577/1184 of 4q9iA

query
sites
4q9iA

Q

Q

T

G

K

N

I

L

A

E

A

F

K

K

N

N

V

I

Q

H

V

F

Y

S

Y

Y

G

P

T

S

-

R

-

K

-

E

T

V

H

Q

A

I

I

L

K

K

D

G

V

L

N

N

V

L

D

K

I

V

L

K

D

S

K

G

T

Q

V

T

T

V

A

A

F

L

I

V

G

G

P

N

S

S

G

G

C

C

G

G

K

K

S

S

T

T

F

T

L

V

R

Q

C

L

L

M

N

Q

R

R

M

L

N

Y

D

D

T

P

I

L

D

D

T

-

A

-

R

-

I

-

E

-

G

G

D

M

I

V

R

S

I

I

D

D

G

G

E

Q

D

D

I

I

Y

-

D

-

R

R

R

T

V

I

D

N

P

V

V

R

Q

Y

L

L

R

R

A

E

K

I

V

I

G

G

M

V

V

V

F

S

Q

Q

K

E

P

P

N

V

P

L

F

F

P

A

K

T

S

T

I

I

Y

A

D

E

N

N

V

I

A

R

Y

Y

G

G

P

-

R

-

I

-

H

-

G

-

L

-

A

-

R

R

T

E

R

D

A

V

E

T

L

M

D

D

E

E

I

I

V

-

E

E

K

K

S

A

L

V

R

K

R

E

A

A

A

N

L

A

W

Y

D

D

-

F

-

I

-

M

-

K

-

L

-

P

-

H

E

Q

A

F

K

D

D

T

R

L

L

V

D

G

Q

E

P

R

G

G

T

A

G

Q

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

V

L

A

V

T

R

S

N

P

P

E

K

V

I

L

L

M

L

D

D

E

E

P

A

C

T

S

S

A

A

L

L

D

D

P

T

I

E

A

S

T

E

A

A

Q

V

V

V

E

Q

E

A

L

A

I

L

D

D

E

K

L

A

R

R

E

E

R

G

Y

R

S

T

V

T

V

I

I

V

V

I

T

A

H

H

S

R

M

L

Q

-

Q

S

A

T

A

V

R

R

V

N

S

A

Q

D

R

V

T

I

A

A

F

G

F

F

H

D

L

G

G

G

H

V

L

I

V

V

E

E

Y

Q

G

G

Sites not aligning to the query:

binding : 39, 199, 270, 274, 303, 310, 634, 885

6q81A Structure of p-glycoprotein(abcb1) in the post-hydrolytic state (see paper)
33% identity, 86% coverage: 14:241/264 of query aligns to 353:574/1182 of 6q81A

query
sites
6q81A

Q

Q

T

G

K

N

I

L

A

E

A

F

K

K

N

N

V

I

Q

H

V

F

Y

S

Y
|
Y

G

P

T

S

-

R

-

K

-
x
E

T

V

H

Q

A

I

I

L

K

K

D

G

V

L

N

N

V

L

D

K

I

V

L

K

D

S

K

G

T

Q

V

T

T

V

A

A

F

L

I

V

G

G

P

N

S

S

G
|
G

C

C

G
|
G

K
|
K

S
|
S

T

T

F

T

L

V

R

Q

C

L

L

M

N

Q

R

R

M

L

N

Y

D

D

T

P

I

L

D

D

T

-

A

-

R

-

I

-

E

-

G

G

D

M

I

V

R

S

I

I

D

D

G

G

E

Q

D

D

I

I

Y

-

D

-

R

R

R

T

V

I

D

N

P

V

V

R

Q

Y

L

L

R

R

A

E

K

I

V

I

G

G

M

V

V

V

F

S

Q

Q

K

E

P

P

N

V

P

L

F

F

P

A

K

T

S

T

I

I

Y

A

D

E

N

N

V

I

A

R

Y

Y

G

G

P

-

R

-

I

-

H

-

G

-

L

-

A

-

R

R

T

E

R

D

A

V

E

T

L

M

D

D

E

E

I

I

V

-

E

E

K

K

S

A

L

V

R

K

R

E

A

A

A

N

L

A

W

Y

D

D

-

F

-

I

-

M

-

K

-

L

-

P

-

H

E

Q

A

F

K

D

D

T

R

L

L

V

D

G

Q

E

P

R

G

G

T

A

G

Q

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

V

L

A

V

T

R

S

N

P

P

E

K

V

I

L

L

M

L

D

D

E

E

P

A

C

T

S

S

A

A

L

L

D

D

P

T

I

E

A

S

T

E

A

A

Q

V

V

V

E

Q

E

A

L

A

I

L

D

D

E

K

L

A

R

R

E

E

R

G

Y

R

S

T

V

T

V

I

I

V

V

I

T

A

H

H

S

R

M

L

Q

-

Q

S

A

T

A

V

R

R

V

N

S

A

Q

D

R

V

T

I

A

A

F

G

F

F

H

D

L

G

G

G

H

V

L

I

V

V

E

E

Y

Q

G

G

binding adenosine-5'-diphosphate: Y365 (= Y26), E370 (vs. gap), G394 (= G52), G396 (= G54), K397 (= K55), S398 (= S56)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 712, 951, 979, 980, 982, 983, 984

3g61A Structure of p-glycoprotein reveals a molecular basis for poly- specific drug binding (see paper)
33% identity, 86% coverage: 14:241/264 of query aligns to 353:574/1182 of 3g61A

query
sites
3g61A

Q

Q

T

G

K

N

I

L

A

E

A

F

K

K

N

N

V

I

Q

H

V

F

Y

S

Y

Y

G

P

T

S

-

R

-

K

-

E

T

V

H

Q

A

I

I

L

K

K

D

G

V

L

N

N

V

L

D

K

I

V

L

K

D

S

K

G

T

Q

V

T

T

V

A

A

F

L

I

V

G

G

P

N

S

S

G

G

C

C

G

G

K

K

S

S

T

T

F

T

L

V

R

Q

C

L

L

M

N

Q

R

R

M

L

N

Y

D

D

T

P

I

L

D

D

T

-

A

-

R

-

I

-

E

-

G

G

D

M

I

V

R

S

I

I

D

D

G

G

E

Q

D

D

I

I

Y

-

D

-

R

R

R

T

V

I

D

N

P

V

V

R

Q

Y

L

L

R

R

A

E

K

I

V

I

G

G

M

V

V

V

F

S

Q

Q

K

E

P

P

N

V

P

L

F

F

P

A

K

T

S

T

I

I

Y

A

D

E

N

N

V

I

A

R

Y

Y

G

G

P

-

R

-

I

-

H

-

G

-

L

-

A

-

R

R

T

E

R

D

A

V

E

T

L

M

D

D

E

E

I

I

V

-

E

E

K

K

S

A

L

V

R

K

R

E

A

A

A

N

L

A

W

Y

D

D

-

F

-

I

-

M

-

K

-

L

-

P

-

H

E

Q

A

F

K

D

D

T

R

L

L

V

D

G

Q

E

P

R

G

G

T

A

G

Q

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

V

L

A

V

T

R

S

N

P

P

E

K

V

I

L

L

M

L

D

D

E

E

P

A

C

T

S

S

A

A

L

L

D

D

P

T

I

E

A

S

T

E

A

A

Q

V

V

V

E

Q

E

A

L

A

I

L

D

D

E

K

L

A

R

R

E

E

R

G

Y

R

S

T

V

T

V

I

I

V

V

I

T

A

H

H

S

R

M

L

Q

-

Q

S

A

T

A

V

R

R

V

N

S

A

Q

D

R

V

T

I

A

A

F

G

F

F

H

D

L

G

G

G

H

V

L

I

V

V

E

E

Y

Q

G

G

Sites not aligning to the query:

binding (4S,11S,18S)-4,11,18-tri(propan-2-yl)-6,13,20-triselena-3,10,17,22,23,24-hexaazatetracyclo[17.2.1.1~5,8~.1~12,15~]tetracosa-1(21),5(24),7,12(23),14,19(22)-hexaene-2,9,16-trione: 39, 268, 271, 300, 307, 635, 639, 860, 885, 889, 889

Query Sequence

>3608131 FitnessBrowser__Dino:3608131
MNDMRLTERAVTEQTKIAAKNVQVYYGTTHAIKDVNVDILDKTVTAFIGPSGCGKSTFLR
CLNRMNDTIDTARIEGDIRIDGEDIYDRRVDPVQLRAKVGMVFQKPNPFPKSIYDNVAYG
PRIHGLARTRAELDEIVEKSLRRAALWDEAKDRLDQPGTGLSGGQQQRLCIARAVATSPE
VLLMDEPCSALDPIATAQVEELIDELRERYSVVIVTHSMQQAARVSQRTAFFHLGHLVEY
GETGHIFTNPEDPRTESYITGRIG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory