SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3608365 FitnessBrowser__Dino:3608365 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

P48636 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; AMP nucleosidase; PaLOG; EC 3.2.2.n1; EC 3.2.2.4 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
49% identity, 98% coverage: 1:176/179 of query aligns to 3:178/195 of P48636

query
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P48636

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E74 (= E72) binding
M89 (= M87) mutation to A: Almost complete loss of activity.
R92 (= R90) mutation to A: Almost complete loss of activity.
RK 92:93 (= RK 90:91) binding
109:115 (vs. 107:113, 57% identical) binding
T112 (≠ S110) mutation to A: Almost complete loss of activity.
E114 (≠ D112) mutation to A: Almost complete loss of activity.
E115 (= E113) mutation to A: Almost complete loss of activity.
E118 (= E116) mutation to A: Retains 8% of wild-type activity.

5zbkA Crystal structure of type-i log from pseudomonas aeruginosa pao1 in complex with amp (see paper)
49% identity, 98% coverage: 1:176/179 of query aligns to 2:177/182 of 5zbkA

query
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5zbkA

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binding adenosine monophosphate: A11 (= A10), E73 (= E72), M88 (= M87), R91 (= R90), K92 (= K91), G108 (= G107), G110 (= G109), T111 (≠ S110)

Q5ZC82 Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG; Protein LONELY GUY; EC 3.2.2.n1 from Oryza sativa subsp. japonica (Rice) (see paper)
46% identity, 89% coverage: 4:163/179 of query aligns to 38:197/242 of Q5ZC82

query
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Q5ZC82

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G73 (= G39) mutation to D: In LOG-4; decreased activity.

5zblD Crystal structure of type-i log from corynebacterium glutamicum in complex with amp (see paper)
39% identity, 98% coverage: 1:176/179 of query aligns to 2:176/181 of 5zblD

query
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5zblD

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binding adenosine monophosphate: S11 (≠ A10), M47 (= M46), R90 (= R90), K91 (= K91), G107 (= G107), G109 (= G109), T110 (≠ S110), E112 (≠ D112), E113 (= E113)

5ajuA Crystal structure of ligand-free phosphoribohydroxylase lonely guy from claviceps purpurea in complex with phosphoribose (see paper)
39% identity, 98% coverage: 2:176/179 of query aligns to 3:200/217 of 5ajuA

query
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5ajuA

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binding 5-O-phosphono-beta-D-ribofuranose: S11 (≠ A10), R108 (= R90), G131 (= G107), Y132 (≠ A108), G133 (= G109), T134 (≠ S110), E137 (= E113)

O05306 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; Protein LONELY GUY homolog; LOG homolog; EC 3.2.2.n1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
36% identity, 99% coverage: 3:179/179 of query aligns to 12:187/187 of O05306

query
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O05306

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M54 (= M46) mutation to A: Large decrease in catalytic activity.
K74 (≠ A66) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup); mutation to A: Abrogates pupylation. This mutant accumulates in M.tuberculosis with a functional proteasome system and sensitizes M.tuberculosis to NO. Decrease in catalytic activity.
E80 (= E72) mutation to A: Large decrease in catalytic activity.
R98 (= R90) mutation to A: Loss of catalytic activity.
T118 (≠ S110) mutation to A: Loss of catalytic activity.
DE 120:121 (= DE 112:113) mutation to AA: Loss of catalytic activity.

7w2iA Crystal structure of log (rv1205) from mycobacterium tuberculosis (see paper)
36% identity, 99% coverage: 3:179/179 of query aligns to 8:183/183 of 7w2iA

query
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7w2iA

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K

Q

V

I

M

M

F

E

A

D

N

R

S

S

D

D

A

A

I

F

V

I

V

V

L

L

P

P

G

G

A

V

G

G

S

T

L

L

D

D

E

E

F

L

F

F

E
x
D

V

A

L

W

T

T

W
x
D

R

G

Q

Y

L

L

G

G

L

T

H

H

A

D

K

K

P

P

I

I

L

V

L

M

L

V

N

D

V

P

D

W

G

G

Y

H

W

F

T

D

P

G

L

L

L

R

A

A

L

W

I

L

D

N

H

G

V

L

I

L

A

D

Q

T

G

G

F

Y

A

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A

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E

P

S

T

L

A

R

M

G

E

F

R

I

L

T

V

V

V

C

V

A

D

D

N

L

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K

A

D

V

A

Q

L

A

R

A

A

L

C

G

A

P

P

A

S

binding magnesium ion: D120 (≠ E116), D124 (≠ W120)

3sbxA Crystal structure of a putative uncharacterized protein from mycobacterium marinum bound to adenosine 5'-monophosphate amp (see paper)
39% identity, 80% coverage: 36:178/179 of query aligns to 35:177/177 of 3sbxA

query
sites
3sbxA

L

L

V

V

Y

W

G

G

A

G

G

G

D

H

V

V

G

S

L

A

M

M

G

G

S

A

V

V

A

S

R

S

A

A

Q

R

Q

A

A

H

G

G

D

W

T

T

F

V

G

G

V

V

I

I

P

P

A

K

H

M

L

L

L

V

D

H

L

R

E
|
E

V

L

G

A

K

D

T

H

D

D

L

A

T

D

R

E

F

L

V

V

T

T

E

E

T

T

M
|
M

H

W

E

E

R
|
R

K
|
K

K

Q

V

V

M

M

F

E

A

D

N

R

S

A

D

N

A

A

I

F

V

I

V

T

L

L

P

P

G

G

G
|
G

A

V

G
|
G

S
x
T

L

L

D
|
D

E
|
E

F

L

E

D

V

V

L

W

T

T

W

E

R

G

Q

Y

L

L

G

G

L

M

H

H

A

D

K

K

P

S

I

I

L

V

L

L

N

D

V

P

D

W

G

G

Y

H

W

F

T

D

P

G

L

L

L

R

A

A

L

W

I

L

D

S

H

E

V

L

I

A

A

D

Q

T

G

G

F

Y

A

V

A

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E

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S

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L

A

R

M

G

E

F

R

I

L

T

I

V

V

C

V

A

D

D

N

L

L

Q

D

A

D

V

A

Q

L

A

Q

A

A

L

C

G

A

P

P

binding adenosine monophosphate: E71 (= E72), M86 (= M87), R89 (= R90), K90 (= K91), G106 (= G107), G108 (= G109), T109 (≠ S110), D111 (= D112), E112 (= E113)

6y01DDD p450 cytochrome, putative (see paper)
29% identity, 68% coverage: 15:136/179 of query aligns to 17:130/166 of 6y01DDD

query
sites
6y01DDD

T

T

P

P

S

T

H

D

T

L

D

K

T

N

A

A

E

Y

A

Q

L

L

G

G

R

Q

W

L

L

I

A

A

E

L

A

E

G

G

H

W

R

V

L

L

V

L

Y

T

G

G

A

G

G

R

D

N

V

V

G

G

L

V

M

M

G

E

S

H

V

A

A

S

R

Q

A

G

A

A

Q

K

A

A

G

E

G

G

D

L

T

T

F

I

G

G

V

I

I

L

P

P

A

S

H

K

L

N

L

T

D

H

L

N

E

V

V

S

G

D

K

A

T

V

D

D

L

I

T

A

R

-

F

-

V

I

V

V

T

T

E

G

T

L

M

G

H

N

E

A

R

R

K

N

V

I

M

N

F

V

A

L

N

S

S

S

D

D

A

V

I

V

V

I

V

A

L

C

P

G

G

I

G
|
G

A

L

G

G

S
x
T

L

L

D

S

E

E

F

V

F

A

E

L

V

A

L

L

T

K

W

-

R

-

Q

-

L

-

G

-

L

-

H

N

A

Q

K

K

P

P

I

V

L

I

L

L

N

N

V

D

D

D

binding chloride ion: G107 (= G107), T110 (≠ S110)

Query Sequence

>3608365 FitnessBrowser__Dino:3608365
MSSICVFCGARAGLTPSHTDTAEALGRWLAEAGHRLVYGAGDVGLMGSVARAAQQAGGDT
FGVIPAHLLDLEVGKTDLTRFVVTETMHERKKVMFANSDAIVVLPGGAGSLDEFFEVLTW
RQLGLHAKPILLLNVDGYWTPLLALIDHVIAQGFAAESLRGFITVCADLQAVQAALGPA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory