SitesBLAST
Comparing 3608582 Dshi_1976 PfkB domain protein (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8cqxA Ribokinase from t.Sp mutant a92g
46% identity, 98% coverage: 5:304/305 of query aligns to 2:298/300 of 8cqxA
- binding adenosine-5'-diphosphate: N179 (= N185), T217 (= T221), G219 (= G223), A220 (= A224), G222 (= G226), F250 (= F256), N272 (≠ C278), G275 (≠ A281), A276 (≠ S282), T279 (≠ V285)
- binding magnesium ion: D242 (≠ E248), T244 (= T250), A278 (≠ S284), S287 (= S293)
4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
41% identity, 100% coverage: 1:305/305 of query aligns to 1:303/306 of 4xckA
- active site: A249 (≠ G251), A250 (= A252), G251 (= G253), D252 (= D254)
- binding adenosine-5'-diphosphate: T220 (= T221), G222 (= G223), S223 (≠ A224), V242 (≠ P243), T247 (= T249), A250 (= A252), F254 (= F256), H276 (≠ C278), A279 (= A281), V283 (= V285)
- binding alpha-D-ribofuranose: N11 (≠ V12), D13 (= D14), G39 (= G40), K40 (= K41), N43 (= N44), A95 (= A95), I107 (= I108), I109 (≠ V110), E140 (= E141), T248 (= T250), D252 (= D254)
P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 3 papers)
42% identity, 98% coverage: 5:304/305 of query aligns to 7:305/309 of P0A9J6
1gqtB Activation of ribokinase by monovalent cations (see paper)
42% identity, 98% coverage: 5:304/305 of query aligns to 6:304/307 of 1gqtB
- active site: A251 (≠ G251), A252 (= A252), G253 (= G253), D254 (= D254)
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N185), T222 (= T221), G224 (= G223), S225 (≠ A224), A252 (= A252), G253 (= G253), H278 (≠ C278), A281 (= A281)
- binding cesium ion: D248 (≠ E248), I250 (≠ T250), A284 (≠ S284), R287 (= R287), S293 (= S293)
- binding alpha-D-ribofuranose: N13 (≠ V12), D15 (= D14), G41 (= G40), N45 (= N44), E142 (= E141), D254 (= D254)
1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121 (see paper)
42% identity, 98% coverage: 5:304/305 of query aligns to 4:302/305 of 1rk2A
- active site: A249 (≠ G251), A250 (= A252), G251 (= G253), D252 (= D254)
- binding adenosine-5'-diphosphate: T220 (= T221), G222 (= G223), S223 (≠ A224), A250 (= A252), G251 (= G253), H276 (≠ C278), A279 (= A281)
- binding tetrafluoroaluminate ion: G213 (= G214), R215 (≠ G216)
- binding magnesium ion: D246 (≠ E248), A282 (≠ S284), R285 (= R287), S291 (= S293)
- binding alpha-D-ribofuranose: N11 (≠ V12), D13 (= D14), G38 (= G39), G39 (= G40), K40 (= K41), N43 (= N44), E140 (= E141), D252 (= D254)
6znxC Ribokinase from thermus species
43% identity, 91% coverage: 27:304/305 of query aligns to 11:263/265 of 6znxC
6wjzA Crystal structure of human ribokinase in complex with ampcp
36% identity, 97% coverage: 5:300/305 of query aligns to 5:304/315 of 6wjzA
- binding phosphomethylphosphonic acid adenosyl ester: N186 (= N185), T222 (= T221), G224 (= G223), A225 (= A224), G227 (= G226), T243 (≠ G244), V246 (vs. gap), A254 (= A252), G255 (= G253), N282 (≠ R275), A285 (= A281), A286 (≠ S282), V289 (= V285)
2fv7A Crystal structure of human ribokinase
37% identity, 97% coverage: 5:300/305 of query aligns to 4:303/308 of 2fv7A
- active site: G252 (= G251), A253 (= A252), G254 (= G253), D255 (= D254)
- binding adenosine-5'-diphosphate: N185 (= N185), T221 (= T221), G223 (= G223), G226 (= G226), T242 (≠ G244), V245 (vs. gap), A253 (= A252), G254 (= G253), N281 (≠ R275), A284 (= A281), A285 (≠ S282), V288 (= V285)
6wk0B Crystal structure of human ribokinase in complex with amppcp and ribose
37% identity, 97% coverage: 5:300/305 of query aligns to 5:304/311 of 6wk0B
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N185), T222 (= T221), G224 (= G223), A225 (= A224), G227 (= G226), T243 (≠ G244), V246 (vs. gap), A254 (= A252), G255 (= G253), N282 (≠ R275), A285 (= A281), A286 (≠ S282), V289 (= V285)
- binding alpha-D-ribofuranose: D14 (= D14), G40 (= G40), K41 (= K41), N44 (= N44), A96 (= A95), E141 (= E141), D256 (= D254)
5c41A Crystal structure of human ribokinase in complex with amppcp in p21 spacegroup and with 4 protomers
37% identity, 97% coverage: 5:300/305 of query aligns to 5:304/317 of 5c41A
- active site: G253 (= G251), A254 (= A252), G255 (= G253), D256 (= D254)
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N185), T222 (= T221), G224 (= G223), A225 (= A224), G227 (= G226), V246 (vs. gap), G255 (= G253), N282 (≠ R275), A285 (= A281), A286 (≠ S282)
5c3yA Structure of human ribokinase crystallized with amppnp
37% identity, 97% coverage: 5:300/305 of query aligns to 4:303/306 of 5c3yA
- active site: G252 (= G251), A253 (= A252), G254 (= G253), D255 (= D254)
- binding amp phosphoramidate: T221 (= T221), G223 (= G223), V245 (vs. gap), T250 (= T249), G254 (= G253), N281 (≠ R275), A284 (= A281), A285 (≠ S282)
5byfA Crystal structure of human ribokinase in complex with amp
37% identity, 96% coverage: 9:300/305 of query aligns to 10:305/313 of 5byfA
Q9H477 Ribokinase; RK; EC 2.7.1.15 from Homo sapiens (Human)
37% identity, 96% coverage: 9:300/305 of query aligns to 22:317/322 of Q9H477
6ilsB Structure of arabidopsis thaliana ribokinase complexed with ribose and atp (see paper)
35% identity, 100% coverage: 1:304/305 of query aligns to 1:309/313 of 6ilsB
- binding adenosine-5'-triphosphate: N189 (= N185), K225 (≠ T221), G227 (= G223), I246 (≠ F241), A248 (≠ P243), A257 (= A252), G258 (= G253), F261 (= F256), A286 (= A281), S287 (= S282)
- binding alpha-D-ribofuranose: N12 (≠ V12), D14 (= D14), G40 (= G40), K41 (= K41), N44 (= N44), E144 (= E141), D259 (= D254)
A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 99% coverage: 4:304/305 of query aligns to 70:375/379 of A1A6H3
Sites not aligning to the query:
- 1:74 mutation Missing: Loss of ribokinase activity.
6a8cA Ribokinase from leishmania donovani with adp (see paper)
32% identity, 98% coverage: 5:302/305 of query aligns to 16:324/327 of 6a8cA
- binding adenosine-5'-diphosphate: G245 (= G223), A246 (= A224), T271 (= T249), A274 (= A252), G275 (= G253), N300 (≠ C278), A303 (= A281)
- binding glycerol: D25 (= D14), S42 (≠ G31), S44 (= S33), G50 (= G39), G51 (= G40), N55 (= N44)
6a8bA Ribokinase from leishmania donovani with amppcp (see paper)
32% identity, 98% coverage: 5:302/305 of query aligns to 16:324/327 of 6a8bA
- binding phosphomethylphosphonic acid adenylate ester: G245 (= G223), A246 (= A224), T271 (= T249), A274 (= A252), G275 (= G253), N300 (≠ C278), A303 (= A281), V307 (= V285)
- binding glycerol: D25 (= D14), G50 (= G39), G51 (= G40), N55 (= N44), N157 (≠ L140), I159 (≠ Q142), E190 (≠ P170)
6a8aA Ribokinase from leishmania donovani with atp (see paper)
32% identity, 98% coverage: 5:302/305 of query aligns to 16:324/327 of 6a8aA
- binding adenosine-5'-triphosphate: N207 (= N185), T243 (= T221), G245 (= G223), A246 (= A224), G248 (= G226), T271 (= T249), G273 (= G251), A274 (= A252), G275 (= G253), N300 (≠ C278), A303 (= A281), V307 (= V285)
- binding glycerol: D25 (= D14), G50 (= G39), G51 (= G40), N55 (= N44)
3ikhA Crystal structure of ribokinase in complex with atp and glycerol in the active site from klebsiella pneumoniae
27% identity, 98% coverage: 5:304/305 of query aligns to 4:285/286 of 3ikhA
3i3yD Crystal structure of ribokinase in complex with d-ribose from klebsiella pneumoniae
27% identity, 98% coverage: 5:304/305 of query aligns to 3:284/285 of 3i3yD
Query Sequence
>3608582 Dshi_1976 PfkB domain protein (RefSeq)
MGKPIVILGIFVADCAFRAARPPRMGETVLGDSFALNPGGKGSNQAIAAAKSGGDARFLT
RLGEDAFADMAAAIWAEAGVRATAPRDPASYTGAAYIYVDPATGDNAIIVAPGAAALLGP
ADLDAQAEMIGGAGVFLTQLEQPMPAARRGLEIARAAGVTTILNPAPAAPLDDALLALCD
IVTPNETEAEALTGHPVTGPDDAVRAADALLARGPGAVIVTLGAQGVLYRSATQTIHQPA
FTPGPVVETTGAGDAFNGALATALAEGQDTASALRFGCATASISVTRPGAGPSMPSRAEI
DALLA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory