SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3608586 Dshi_1980 ABC transporter related (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1ji0A Crystal structure analysis of the abc transporter from thermotoga maritima
38% identity, 92% coverage: 14:234/240 of query aligns to 15:238/240 of 1ji0A

query
sites
1ji0A

Y

Y

R
x
Y

P

G

G

A

L

I

P

H

I

A

L

I

H

K

G

G

V

I

S

D

L

L

R

K

A

V

R

P

A

R

A

G

R

Q

V

I

T

V

V

T

I

L

I

I

G

G

P

A

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

L

K

S

A

A

I

I

A

A

G

G

L

L

V

V

P

R

V

A

T

Q

S

K

G

G

T

K

I

I

A

I

A

F

E

N

-

G

T

Q

E

D

I

I

T

T

G

N

L

K

R

P

P

A

D

H

L

V

L

I

A

N

R

R

H

M

G

G

I

I

A

A

Y

L

V

V

P

P

Q
x
E

S

G

D

R

N

R

I

I

F

F

R

P

T

E

L

L

T

T

V

V

R

Y

E

E

N

N

L

L

D

M

L

M

V

G

L

A

R

Y

H

N

V

R

R

K

A

D

D

K

A

E

P

G

A

I

R

K

R

R

-

D

-

L

A

E

G

W

L

I

L

F

D

S

Q

L

F

F

P

P

V

R

L

L

A

K

D

E

K

R

L

L

G

K

D

Q

K

L

A

G

G

G

A

T

L

L

S

S

G

G

G

G

Q

E

R

Q

Q

Q

F

M

L

L

A

A

V

I

A

G

M

R

A

A

L

L

A

M

T

S

A

R

P

P

S

K

V

L

I

L

L

M

M

M

D

D

E
|
E

P

P

S

S

A

L

G

G

L

L

A

A

P

P

K

I

A

L

A

V

Q

S

E

E

V

V

L

F

E

E

H

V

A

I

R

Q

A

K

L

I

T

N

E

Q

T

E

G

G

V

T

T

T

I

I

L

L

L

L

V

V

E

E

Q

Q

N

N

V

A

K

L

Q

G

A

A

L

L

R

K

L

V

S

A

D

H

H

Y

C

G

Y

Y

I

V

L

L

A

E

D

T

G

G

R

Q

N

I

Q

V

I

L

D

E

G

G

P

K

A

A

A

S

D

E

L

L

L

L

D

D

D

N

P

E

V

M

V

V

G

R

Q

K

I

A

Y

Y

L

L

G

G

binding adenosine-5'-triphosphate: Y16 (≠ R15), G42 (= G41), G44 (= G43), K45 (= K44), T46 (≠ S45), T47 (= T46), E88 (≠ Q86), E165 (= E161)

1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
33% identity, 95% coverage: 8:234/240 of query aligns to 7:253/254 of 1g6hA

query
sites
1g6hA

T

T

D

E

L

N

V

I

A

V

G

K

Y

Y

R
x
F

P

G

G

E

L
x
F

P

K

I

A

L

L

H

D

G

G

V

V

S

S

L

I

R

S

A

V

R

N

A

K

A

G

R

D

V

V

T

T

V

L

I

I

I

I

G

G

P

P

N
|
N

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

N

A

V

I

I

A

T

G

G

L

F

V

L

P

K

V

A

T

D

S

E

G

G

T

R

I

V

A

Y

A

F

E

E

T

N

-

K

E

D

I

I

T

T

G

N

L

K

R

E

P

P

D

A

L

E

L

L

A

Y

R

H

H

Y

G

G

I

I

A

V

Y

R

V

T

P

F

Q

Q

S

T

D

P

N

Q

I

P

F

L

R

K

T

E

L

M

T

T

V

V

R

L

E

E

N

N

L

L

-

L

-

I

-

G

-

E

-

I

-

C

-

P

-

G

-

E

-

S

-

P

-

L

-

N

D

S

L

L

V

F

L

Y

R

K

H

K

V

W

-

I

-

P

R

K

A

E

D

E

A

E

P

M

A

V

R

E

R

K

A

A

G

F

L

K

L

I

D

L

Q

E

F

F

P

L

V

K

L

L

A

S

D

H

K

L

L

Y

G

D

D

R

K

K

A

A

G

G

A

E

L

L

S

S

G

G

G

G

Q

Q

R

M

Q

K

F

L

L

V

A

E

V

I

A

G

M

R

A

A

L

L

A

M

T

T

A

N

P

P

S

K

V

M

I

I

L

V

M

M

D

D

E

E

P

P

S

I

A

A

G

G

L

V

A

A

P

P

K

G

A

L

A

A

Q

H

E

D

V

I

L

F

E

N

H

H

A

V

R

L

A

E

L

L

T

K

E

A

T

K

G

G

V

I

T

T

I

F

L

L

L

I

V

I

E

E

Q

H

N

R

V

L

K

D

Q

I

A

V

L

L

R

N

L

Y

S

I

D

D

H

H

C

L

Y

Y

I

V

L

M

A

F

D

N

G

G

R

Q

N

I

Q

I

I

A

D

E

G

G

-

R

-

G

-

E

-

E

P

E

A

I

A

K

D

N

L

V

L

L

D

S

D

D

P

P

V

K

V

V

G

V

Q

E

I

I

Y

Y

L

I

G

G

binding adenosine-5'-diphosphate: F14 (≠ R15), F17 (≠ L18), N39 (= N40), G40 (= G41), G42 (= G43), K43 (= K44), S44 (= S45), T45 (= T46)

1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
33% identity, 95% coverage: 8:234/240 of query aligns to 7:253/253 of 1g9xB

query
sites
1g9xB

T

T

D

E

L

N

V

I

A

V

G

K

Y

Y

R
x
F

P

G

G

E

L
x
F

P

K

I

A

L

L

H

D

G

G

V

V

S

S

L

I

R

S

A

V

R

C

A

K

A

G

R

D

V

V

T

T

V

L

I

I

I

I

G

G

P

P

N

N

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

N

A

V

I

I

A

T

G

G

L

F

V

L

P

K

V

A

T

D

S

E

G

G

T

R

I

V

A

Y

A

F

E

E

T

N

-

K

E

D

I

I

T

T

G

N

L

K

R

E

P

P

D

A

L

E

L

L

A

Y

R

H

H

Y

G

G

I

I

A

V

Y

R

V

T

P

F

Q

Q

S

T

D

P

N

Q

I

P

F

L

R

K

T

E

L

M

T

T

V

V

R

L

E

E

N

N

L

L

-

L

-

I

-

G

-

E

-

I

-

N

-

P

-

G

-

E

-

S

-

P

-

L

-

N

D

S

L

L

V

F

L

Y

R

K

H

K

V

W

-

I

-

P

R

K

A

E

D

E

A

E

P

M

A

V

R

E

R

K

A

A

G

F

L

K

L

I

D

L

Q

E

F

F

P

L

V

K

L

L

A

S

D

H

K

L

L

Y

G

D

D

R

K

K

A

A

G

G

A

E

L

L

S

S

G

G

G

G

Q

Q

R

M

Q

K

F

L

L

V

A

E

V

I

A

G

M

R

A

A

L

L

A

M

T

T

A

N

P

P

S

K

V

M

I

I

L

V

M

M

D

D

E
|
E

P

P

S

I

A

A

G

G

L

V

A

A

P

P

K

G

A

L

A

A

Q

H

E

D

V

I

L

F

E

N

H

H

A

V

R

L

A

E

L

L

T

K

E

A

T

K

G

G

V

I

T

T

I

F

L

L

L

I

V

I

E

E

Q

H

N

R

V

L

K

D

Q

I

A

V

L

L

R

N

L

Y

S

I

D

D

H

H

C

L

Y

Y

I

V

L

M

A

F

D

N

G

G

R

Q

N

I

Q

I

I

A

D

E

G

G

-

R

-

G

-

E

-

E

P

E

A

I

A

K

D

N

L

V

L

L

D

S

D

D

P

P

V

K

V

V

G

V

Q

E

I

I

Y

Y

L

I

G

G

binding adenosine-5'-diphosphate: F14 (≠ R15), F17 (≠ L18), G40 (= G41), S41 (≠ A42), G42 (= G43), K43 (= K44), S44 (= S45), T45 (= T46)
binding magnesium ion: S44 (= S45), E176 (= E161)

6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
35% identity, 96% coverage: 5:234/240 of query aligns to 3:235/235 of 6mhzA

query
sites
6mhzA

L

L

T

T

I

A

T

K

D

N

L

L

V

A

A

K

G

A

Y
|
Y

R

K

P

-

G

G

L

R

P

R

I

V

L

V

H

E

G

D

V

V

S

S

L

L

R

T

A

V

R

N

A

S

A

G

R

E

V

I

T

V

V

G

I

L

I

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

A

M

I

V

A

V

G

G

L

I

V

V

P

P

V

R

T

D

S

A

G

G

T

N

I

I

A

I

A

I

E

D

T

D

E

D

I

D

T

I

G

S

L

L

R

L

P

P

-

L

D

H

L

A

L

R

A

A

R

R

H

R

G

G

I

I

A

G

Y

Y

V

L

P

P

Q
|
Q

S

E

D

A

N

S

I

I

F

F

R

R

T

R

L

L

T

S

V

V

R

Y

E

D

N

N

L

L

D

M

L

A

V

V

L

L

R

Q

H

-

V

I

R

R

A

D

D

D

A

L

P

S

A

A

-

E

-

Q

-

R

-

E

-

D

R

R

R

A

A

N

G

E

L

L

L

M

D

E

Q

E

F

F

P

H

V

I

L

-

A

-

D

E

K

H

L

L

G

R

D

D

K

S

A

M

G

G

-

Q

A
x
S

L

L

S
|
S

G
|
G

G
|
G

Q

E

R

R

Q

R

F

R

L

V

A

E

V

I

A

A

M

R

A

A

L

L

A

A

T

A

A

N

P

P

S

K

V

F

I

I

L

L

M

L

D

D

E
|
E

P

P

S

F

A

A

G
|
G

L

V

A

D

P

P

K

I

A

S

A

V

-

I

-

D

-

I

Q

K

E

R

V

I

L

I

E

E

H

H

A

L

R

R

A

-

L

-

T

-

E

D

T

S

G

G

V

L

T

G

I

V

L

L

L

I

V

T

E

D

Q
x
H

N

N

V

V

K

R

Q

E

A

T

L

L

R

A

L

V

S

C

D

E

H

R

C

A

Y

Y

I

I

L

V

A

S

D

Q

G

G

R

H

N

L

Q

I

I

A

D

H

G

G

P

T

A

P

A

T

D

E

L

I

L

L

D

Q

D

D

P

E

V

H

V

V

G

K

Q

R

I

V

Y

Y

L

L

G

G

binding adp orthovanadate: Y12 (= Y14), N37 (= N40), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46), Q84 (= Q86), S136 (≠ A135), S138 (= S137), G139 (= G138), G140 (= G139), E162 (= E161), G166 (= G165), H194 (≠ Q193)

6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
35% identity, 96% coverage: 5:234/240 of query aligns to 3:235/238 of 6s8nB

query
sites
6s8nB

L

L

T

T

I

A

T

K

D

N

L

L

V

A

A

K

G

A

Y

Y

R

K

P

-

G

G

L

R

P

R

I

V

L

V

H

E

G

D

V

V

S

S

L

L

R

T

A

V

R

N

A

S

A

G

R

E

V

I

T

V

V

G

I

L

I

L

G

G

P

P

N

N

G

G

A

A

G

G

K

K

S

T

T

T

L

T

I

F

K

Y

A

M

I

V

A

V

G

G

L

I

V

V

P

P

V

R

T

D

S

A

G

G

T

N

I

I

A

I

A

I

E

D

T

D

E

D

I

D

T

I

G

S

L

L

R

L

P

P

-

L

D

H

L

A

L

R

A

A

R

R

H

R

G

G

I

I

A

G

Y

Y

V

L

P

P

Q

Q

S

E

D

A

N

S

I

I

F

F

R
|
R

T

R

L

L

T

S

V

V

R

Y

E

D

N

N

L

L

D

M

L

A

V

V

L

L

R

Q

H

-

V

I

R

R

A

D

D

D

A

L

P

S

A

A

-

E

-

Q

-

R

-

E

-

D

R

R

R

A

A

N

G

E

L

L

L

M

D

E

Q

E

F

F

P

H

V

I

L

-

A

-

D

E

K

H

L

L

G

R

D

D

K

S

A
x
M

G
|
G

-
x
Q

A

S

L

L

S

S

G

G

G

G

Q

E

R

R

Q

R

F

R

L

V

A

E

V

I

A

A

M

R

A

A

L

L

A

A

T

A

A

N

P

P

S

K

V

F

I

I

L

L

M

L

D

D

E

E

P

P

S

F

A

A

G

G

L

V

A

D

P

P

K

I

A

S

A

V

-

I

-

D

-

I

Q

K

E

R

V

I

L

I

E

E

H

H

A

L

R

R

A

-

L

-

T

-

E

D

T

S

G

G

V

L

T

G

I

V

L

L

L

I

V

T

E

D

Q

H

N

N

V

V

K

R

Q

E

A

T

L

L

R

A

L

V

S

C

D

E

H

R

C

A

Y

Y

I

I

L

V

A

S

D

Q

G

G

R

H

N

L

Q

I

I

A

D

H

G

G

P

T

A

P

A

T

D

E

L

I

L

L

D

Q

D

D

P

E

V

H

V

V

G

K

Q

R

I

V

Y

Y

L

L

G

G

binding 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione: R90 (= R92), M133 (≠ A133), G134 (= G134), Q135 (vs. gap)

6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
35% identity, 96% coverage: 5:234/240 of query aligns to 3:235/238 of 6s8gA

query
sites
6s8gA

L

L

T

T

I

A

T

K

D

N

L

L

V

A

A

K

G

A

Y
|
Y

R

K

P

-

G

G

L
x
R

P

R

I

V

L

V

H

E

G

D

V

V

S

S

L

L

R

T

A

V

R

N

A

S

A

G

R

E

V

I

T

V

V

G

I

L

I

L

G

G

P

P

N
|
N

G

G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

A

M

I

V

A

V

G

G

L

I

V

V

P

P

V

R

T

D

S

A

G

G

T

N

I

I

A

I

A

I

E

D

T

D

E

D

I

D

T

I

G

S

L

L

R

L

P

P

-

L

D

H

L

A

L

R

A

A

R

R

H

R

G

G

I

I

A

G

Y

Y

V

L

P

P

Q
|
Q

S

E

D

A

N

S

I

I

F

F

R

R

T

R

L

L

T

S

V

V

R

Y

E

D

N

N

L

L

D

M

L

A

V

V

L

L

R

Q

H

-

V

I

R

R

A

D

D

D

A

L

P

S

A

A

-

E

-

Q

-

R

-

E

-

D

R

R

R

A

A

N

G

E

L

L

L

M

D

E

Q

E

F

F

P

H

V

I

L

-

A

-

D

E

K

H

L

L

G

R

D

D

K

S

A

M

G

G

-

Q

A
x
S

L

L

S
|
S

G

G

G

G

Q
x
E

R

R

Q

R

F

R

L

V

A

E

V

I

A

A

M

R

A

A

L

L

A

A

T

A

A

N

P

P

S

K

V

F

I

I

L

L

M

L

D

D

E

E

P

P

S

F

A

A

G

G

L

V

A

D

P

P

K

I

A

S

A

V

-

I

-

D

-

I

Q

K

E

R

V

I

L

I

E

E

H

H

A

L

R

R

A

-

L

-

T

-

E

D

T

S

G

G

V

L

T

G

I

V

L

L

L

I

V

T

E

D

Q

H

N

N

V

V

K

R

Q

E

A

T

L

L

R

A

L

V

S

C

D

E

H

R

C

A

Y

Y

I

I

L

V

A

S

D

Q

G

G

R

H

N

L

Q

I

I

A

D

H

G

G

P

T

A

P

A

T

D

E

L

I

L

L

D

Q

D

D

P

E

V

H

V

V

G

K

Q

R

I

V

Y

Y

L

L

G

G

binding phosphoaminophosphonic acid-adenylate ester: Y12 (= Y14), R15 (≠ L18), N37 (= N40), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46), Q84 (= Q86), S136 (≠ A135), S138 (= S137), E141 (≠ Q140)

6mbnA Lptb e163q in complex with atp (see paper)
35% identity, 96% coverage: 5:234/240 of query aligns to 4:236/241 of 6mbnA

query
sites
6mbnA

L

L

T

T

I

A

T

K

D

N

L

L

V

A

A

K

G

A

Y
|
Y

R

K

P

-

G

G

L
x
R

P

R

I
x
V

L

V

H

E

G

D

V

V

S

S

L

L

R

T

A

V

R

N

A

S

A

G

R

E

V

I

T

V

V

G

I

L

I

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

A

M

I

V

A

V

G

G

L

I

V

V

P

P

V

R

T

D

S

A

G

G

T

N

I

I

A

I

A

I

E

D

T

D

E

D

I

D

T

I

G

S

L

L

R

L

P

P

-

L

D

H

L

A

L

R

A

A

R

R

H

R

G

G

I

I

A

G

Y

Y

V

L

P

P

Q

Q

S

E

D

A

N

S

I

I

F

F

R

R

T

R

L

L

T

S

V

V

R

Y

E

D

N

N

L

L

D

M

L

A

V

V

L

L

R

Q

H

-

V

I

R

R

A

D

D

D

A

L

P

S

A

A

-

E

-

Q

-

R

-

E

-

D

R

R

R

A

A

N

G

E

L

L

L

M

D

E

Q

E

F

F

P

H

V

I

L

-

A

-

D

E

K

H

L

L

G

R

D

D

K

S

A

M

G

G

-

Q

A

S

L

L

S

S

G

G

G

G

Q

E

R

R

Q

R

F

R

L

V

A

E

V

I

A

A

M

R

A

A

L

L

A

A

T

A

A

N

P

P

S

K

V

F

I

I

L

L

M

L

D

D

E

Q

P

P

S

F

A

A

G

G

L

V

A

D

P

P

K

I

A

S

A

V

-

I

-

D

-

I

Q

K

E

R

V

I

L

I

E

E

H

H

A

L

R

R

A

-

L

-

T

-

E

D

T

S

G

G

V

L

T

G

I

V

L

L

L

I

V

T

E

D

Q
x
H

N

N

V

V

K

R

Q

E

A

T

L

L

R

A

L

V

S

C

D

E

H

R

C

A

Y

Y

I

I

L

V

A

S

D

Q

G

G

R

H

N

L

Q

I

I

A

D

H

G

G

P

T

A

P

A

T

D

E

L

I

L

L

D

Q

D

D

P

E

V

H

V

V

G

K

Q

R

I

V

Y

Y

L

L

G

G

binding adenosine-5'-triphosphate: Y13 (= Y14), R16 (≠ L18), V18 (≠ I20), N38 (= N40), G39 (= G41), G41 (= G43), K42 (= K44), T43 (≠ S45), T44 (= T46), H195 (≠ Q193)

6b89A E. Coli lptb in complex with adp and novobiocin (see paper)
35% identity, 95% coverage: 5:233/240 of query aligns to 3:234/234 of 6b89A

query
sites
6b89A

L

L

T

T

I

A

T

K

D

N

L

L

V

A

A

K

G

A

Y
|
Y

R

K

P

-

G

G

L
x
R

P

R

I
x
V

L

V

H

E

G

D

V

V

S

S

L

L

R

T

A

V

R

N

A

S

A

G

R

E

V

I

T

V

V

G

I

L

I

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

A

M

I

V

A

V

G

G

L

I

V

V

P

P

V

R

T

D

S

A

G

G

T

N

I

I

A

I

A

I

E

D

T

D

E

D

I

D

T

I

G

S

L

L

R

L

P

P

-
x
L

D
x
H

L

A

L

R

A

A

R

R

H

R

G

G

I

I

A

G

Y

Y

V

L

P
|
P

Q
|
Q

S

E

D
x
A

N
x
S

I

I

F
|
F

R
|
R

T
x
R

L
|
L

T

S

V

V

R

Y

E

D

N

N

L

L

D

M

L

A

V
|
V

L

L

R

Q

H

-

V

I

R

R

A

D

D

D

A

L

P

S

A

A

-

E

-

Q

-

R

-

E

-

D

R

R

R

A

A

N

G

E

L

L

L

M

D

E

Q

E

F

F

P

H

V

I

L

-

A

-

D

E

K

H

L

L

G

R

D

D

K

S

A

M

G

G

-
x
Q

A

S

L

L

S

S

G

G

G

G

Q

E

R

R

Q

R

F

R

L

V

A

E

V

I

A

A

M
x
R

A

A

L

L

A

A

T

A

A

N

P

P

S

K

V

F

I

I

L

L

M

L

D

D

E

E

P

P

S

F

A

A

G

G

L

V

A

D

P

P

K

I

A

S

A

V

-

I

-

D

-

I

Q

K

E

R

V

I

L

I

E

E

H

H

A

L

R

R

A

-

L

-

T

-

E

D

T

S

G

G

V

L

T

G

I

V

L

L

L

I

V

T

E

D

Q

H

N

N

V

V

K

R

Q

E

A

T

L

L

R

A

L

V

S

C

D

E

H

R

C

A

Y

Y

I

I

L

V

A

S

D

Q

G

G

R

H

N

L

Q

I

I

A

D

H

G

G

P

T

A

P

A

T

D

E

L

I

L

L

D

Q

D

D

P

E

V

H

V

V

G

K

Q

R

I

V

Y

Y

L

L

binding adenosine-5'-diphosphate: Y12 (= Y14), R15 (≠ L18), V17 (≠ I20), N37 (= N40), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46)
binding magnesium ion: T42 (≠ S45), Q84 (= Q86)
binding novobiocin: L71 (vs. gap), H72 (≠ D74), P83 (= P85), A86 (≠ D88), S87 (≠ N89), F89 (= F91), R90 (= R92), R91 (≠ T93), L92 (= L94), V101 (= V103), Q135 (vs. gap), R149 (≠ M148)

4p31A Crystal structure of a selenomethionine derivative of e. Coli lptb in complex with adp-magensium (see paper)
35% identity, 95% coverage: 5:233/240 of query aligns to 3:234/234 of 4p31A

query
sites
4p31A

L

L

T

T

I

A

T

K

D

N

L

L

V

A

A

K

G

A

Y
|
Y

R

K

P

-

G

G

L
x
R

P

R

I
x
V

L

V

H

E

G

D

V

V

S

S

L

L

R

T

A

V

R

N

A

S

A

G

R

E

V

I

T

V

V

G

I

L

I

L

G

G

P

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

A

M

I

V

A

V

G

G

L

I

V

V

P

P

V

R

T

D

S

A

G

G

T

N

I

I

A

I

A

I

E

D

T

D

E

D

I

D

T

I

G

S

L

L

R

L

P

P

-

L

D

H

L

A

L

R

A

A

R

R

H

R

G

G

I

I

A

G

Y

Y

V

L

P

P

Q
|
Q

S

E

D

A

N

S

I

I

F

F

R

R

T

R

L

L

T

S

V

V

R

Y

E

D

N

N

L

L

D

M

L

A

V

V

L

L

R

Q

H

-

V

I

R

R

A

D

D

D

A

L

P

S

A

A

-

E

-

Q

-

R

-

E

-

D

R

R

R

A

A

N

G

E

L

L

L

M

D

E

Q

E

F

F

P

H

V

I

L

-

A

-

D

E

K

H

L

L

G

R

D

D

K

S

A

M

G

G

-

Q

A

S

L

L

S

S

G

G

G

G

Q

E

R

R

Q

R

F

R

L

V

A

E

V

I

A

A

M

R

A

A

L

L

A

A

T

A

A

N

P

P

S

K

V

F

I

I

L

L

M

L

D

D

E

E

P

P

S

F

A

A

G

G

L

V

A

D

P

P

K

I

A

S

A

V

-

I

-

D

-

I

Q

K

E

R

V

I

L

I

E

E

H

H

A

L

R

R

A

-

L

-

T

-

E

D

T

S

G

G

V

L

T

G

I

V

L

L

L

I

V

T

E

D

Q

H

N

N

V

V

K

R

Q

E

A

T

L

L

R

A

L

V

S

C

D

E

H

R

C

A

Y

Y

I

I

L

V

A

S

D

Q

G

G

R

H

N

L

Q

I

I

A

D

H

G

G

P

T

A

P

A

T

D

E

L

I

L

L

D

Q

D

D

P

E

V

H

V

V

G

K

Q

R

I

V

Y

Y

L

L

binding adenosine-5'-diphosphate: Y12 (= Y14), R15 (≠ L18), V17 (≠ I20), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46)
binding magnesium ion: T42 (≠ S45), Q84 (= Q86)

6b8bA E. Coli lptb in complex with adp and a novobiocin derivative (see paper)
34% identity, 95% coverage: 5:232/240 of query aligns to 3:233/233 of 6b8bA

query
sites
6b8bA

L

L

T

T

I

A

T

K

D

N

L

L

V

A

A

K

G

A

Y
|
Y

R

K

P

-

G

G

L
x
R

P

R

I
x
V

L

V

H

E

G

D

V

V

S

S

L

L

R

T

A

V

R

N

A

S

A

G

R

E

V

I

T

V

V

G

I

L

I

L

G

G

P

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

A

M

I

V

A

V

G

G

L

I

V

V

P

P

V

R

T

D

S

A

G

G

T

N

I

I

A

I

A

I

E

D

T

D

E

D

I

D

T

I

G

S

L

L

R

L

P

P

-

L

D

H

L

A

L

R

A

A

R

R

H

R

G

G

I

I

A

G

Y

Y

V

L

P

P

Q

Q

S

E

D

A

N

S

I

I

F
|
F

R
|
R

T
x
R

L

L

T

S

V

V

R

Y

E

D

N

N

L

L

D

M

L

A

V

V

L

L

R

Q

H

-

V

I

R

R

A

D

D

D

A

L

P

S

A

A

-

E

-

Q

-

R

-

E

-

D

R

R

R

A

A

N

G

E

L

L

L

M

D

E

Q

E

F

F

P

H

V

I

L

-

A

-

D

E

K

H

L

L

G

R

D

D

K

S

A

M

G

G

-

Q

A

S

L

L

S

S

G

G

G

G

Q

E

R

R

Q

R

F

R

L

V

A

E

V

I

A

A

M

R

A

A

L

L

A

A

T

A

A

N

P

P

S

K

V

F

I

I

L

L

M

L

D

D

E

E

P

P

S

F

A

A

G

G

L

V

A

D

P

P

K

I

A

S

A

V

-

I

-

D

-

I

Q

K

E

R

V

I

L

I

E

E

H

H

A

L

R

R

A

-

L

-

T

-

E

D

T

S

G

G

V

L

T

G

I

V

L

L

L

I

V

T

E

D

Q

H

N

N

V

V

K

R

Q

E

A

T

L

L

R

A

L

V

S

C

D

E

H

R

C

A

Y

Y

I

I

L

V

A

S

D

Q

G

G

R

H

N

L

Q

I

I

A

D

H

G

G

P

T

A

P

A

T

D

E

L

I

L

L

D

Q

D

D

P

E

V

H

V

V

G

K

Q

R

I

V

Y

Y

binding adenosine-5'-diphosphate: Y12 (= Y14), R15 (≠ L18), V17 (≠ I20), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46)
binding (3s,5s,7s)-N-{7-[(3-O-carbamoyl-6-deoxy-5-methyl-4-O-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl}tricyclo[3.3.1.1~3,7~]decane-1-carboxamide: F89 (= F91), R90 (= R92), R91 (≠ T93)

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
34% identity, 90% coverage: 20:234/240 of query aligns to 17:235/240 of 6mjpA

query
sites
6mjpA

I

V

L

V

H

S

G

D

V

V

S

S

L

L

R

Q

A

V

R

E

A

S

A

G

R

Q

V

I

T

V

V

G

I

L

I

L

G

G

P

P

N

N

G

G

A

A

G

G

K

K

S

T

T

T

L

S

I

F

K

Y

A

M

I

I

A

V

G

G

L

L

V

V

P

A

V

R

T

D

S

E

G

G

T

T

I

I

A

T

A

I

E

D

-

D

T

N

E

D

I

I

T

S

G

I

L

L

R

P

P

M

D

H

L

S

L

R

A

S

R

R

H

M

G

G

I

I

A

G

Y

Y

V

L

P

P

Q

Q

S

E

D

A

N

S

I

I

F

F

R

R

T

K

L

L

T

S

V

V

R

E

E

D

N

N

L

I

D

M

L

A

V

V

L

L

R

Q

H

T

-

R

-

E

-

E

-

L

V

T

R

H

A
x
E

D

E

A

R

P

Q

A
x
D

R

K

R

L

A

E

G

D

L

L

L

L

D

E

Q

E

F

F

P

H

V

I

L

-

A

-

D

Q

K

H

L

I

G

R

D

K

K

S

A

A

G

G

-

M

A

A

L

L

S

S

G

G

G

G

Q

E

R

R

Q

R

F

R

L

V

A

E

V

I

A

A

M

R

A

A

L

L

A

A

T

A

A

N

P

P

S

Q

V

F

I

I

L

L

M

L

D

D

E

Q

P

P

S

F

A

A

G

G

L

V

A

D

P

P

K

I

A

S

A

V

-

I

-

D

-

I

Q

K

E

K

V

I

L

I

E

E

H

H

A

L

R

R

A

-

L

-

T

-

E

D

T

R

G

G

V

L

T

G

I

V

L

L

L

I

V

T

E

D

Q

H

N

N

V

V

K

R

Q

E

A

T

L

L

R

D

L

V

S

C

D

E

H

K

C

A

Y

Y

I

I

L

V

A

S

D

Q

G

G

R

R

N

L

Q

I

I

A

D

E

G

G

P

T

A

P

A

Q

D

D

L

V

L

L

D

N

D

N

P

E

V

Q

V

V

G

K

Q

Q

I

V

Y

Y

L

L

G

G

binding calcium ion: E111 (≠ A109), D115 (≠ A113)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
34% identity, 81% coverage: 18:211/240 of query aligns to 14:212/240 of 4ymuJ

query
sites
4ymuJ

L

L

P

E

I
x
V

L

L

H

K

G

G

V

V

S

T

L

L

R

K

A

V

R

N

A

K

A

G

R

E

V

V

T

V

V

V

I

I

I

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

A

C

I

I

A

N

G

L

L

L

V

E

P

E

V

P

T

T

S

K

G

G

T

E

I

V

A

F

A

I

E

D

-

G

T

V

E

K

I

I

T

N

G

N

L

G

R

K

P

V

D

N

L

I

-

N

L

K

A

V

R

R

H

Q

G

K

I

V

A

G

Y

M

V

V

P

F

Q

Q

S

H

D

F

N

N

I

L

F

F

R

P

T

H

L

L

T

T

V

A

R

I

E

E

N

N

L

I

D

T

L

L

-

A

-

P

-

V

-

K

V

V

L

K

R

K

H

M

V

N

R

K

A

K

D

E

A

A

P

E

A

E

-

L

-

A

-

V

-

D

-

L

-

L

R

A

R

K

A

V

G

G

L

L

L

L

D

D

-

K

-

K

-

D

Q

Q

F

Y

P

P

V

I

L

-

A

-

D

-

K

-

L

-

G

-

D

-

K

-

A

-

G

-

A

K

L

L

S

S

G

G

G

G

Q

Q

R

K

Q

Q

F

R

L

L

A

A

V

I

A

A

M

R

A

A

L

L

A

A

T

M

A

Q

P

P

S

E

V

V

I

M

L

L

M

F

D

D

E
|
E

P

P

S

T

A

S

G

A

L

L

A

D

P

P

K

E

A

M

A

V

Q

K

E

E

V

V

L

L

E

N

H

V

A

M

R

K

A

Q

L

L

T

A

E

N

T

E

G

G

V

M

T

T

I

M

L

V

L

V

V

V

E

T

Q
x
H

N

E

V

M

K

G

Q

F

A

A

L

R

R

E

L

V

S

G

D

D

H

R

C

V

Y

I

I

F

L

M

A

D

D

D

G

G

binding adenosine-5'-triphosphate: V16 (≠ I20), S36 (≠ N40), G37 (= G41), S38 (≠ A42), G39 (= G43), K40 (= K44), S41 (= S45), T42 (= T46), E162 (= E161), H194 (≠ Q193)
binding magnesium ion: S41 (= S45), E162 (= E161)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

3c4jA Abc protein artp in complex with atp-gamma-s
32% identity, 87% coverage: 18:226/240 of query aligns to 16:229/242 of 3c4jA

query
sites
3c4jA

L

L

P

E

I
x
V

L

L

H

K

G

G

V

I

S

N

L

V

R

H

A

I

R

R

A

E

A

G

R

E

V

V

T

V

V

V

I

V

I

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

A

C

I

L

A

N

G

L

L

L

V

E

P

D

V

F

T

D

S

E

G

G

T

E

I

I

-

I

-

I

-

D

-

G

-

I

-

N

-

L

-

K

A

A

A

K

E

D

T

T

E

N

I

L

T

N

G

K

L

V

R

R

P

E

D

E

L

-

L

-

A

-

R

-

H

-

G

-

I

V

A

G

Y

M

V

V

P

F

Q

Q

S

R

D

F

N

N

I

L

F

F

R

P

T

H

L

M

T

T

V

V

R

L

E

N

N

N

L

I

D

T

L

L

-

A

-

P

-

M

-

K

V

V

L

R

R

K

H

W

V

P

R

R

A

E

D

K

A

A

P

E

A

A

R

K

R

A

A

M

G

E

L

L

L

L

D

D

Q

K

F

V

P

G

V

-

L

L

A

K

D

D

K

K

L

A

G

H

D

A

K

Y

A

P

G

D

A

S

L

L

S

S

G

G

G

G

Q

Q

R

A

Q

Q

F

R

L

V

A

A

V

I

A

A

M

R

A

A

L

L

A

A

T

M

A

E

P

P

S

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

S

T

A

S

G

A

L

L

A

D

P

P

K

E

A

M

A

V

Q

G

E

E

V

V

L

L

E

S

H

V

A

M

R

K

A

Q

L

L

T

A

E

N

T

E

G

G

V

M

T

T

I

M

L

V

L

V

V

V

E

T

Q

H

N

E

V

M

K

G

Q

F

A

A

L

R

R

E

L

V

S

G

D

D

H

R

C

V

Y

L

I

F

L

M

A

D

D

G

G

G

R

Y

N

I

Q

I

I

E

D

E

G

G

P

K

A

P

A

E

D

D

L

L

L

F

D

D

D

R

P

P

binding phosphothiophosphoric acid-adenylate ester: V18 (≠ I20), S38 (≠ N40), G39 (= G41), S40 (≠ A42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 13

3c41J Abc protein artp in complex with amp-pnp/mg2+
32% identity, 87% coverage: 18:226/240 of query aligns to 16:229/242 of 3c41J

query
sites
3c41J

L

L

P

E

I
x
V

L

L

H

K

G

G

V

I

S

N

L

V

R

H

A

I

R

R

A

E

A

G

R

E

V

V

T

V

V

V

I

V

I

I

G

G

P

P

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

A

C

I

L

A

N

G

L

L

L

V

E

P

D

V

F

T

D

S

E

G

G

T

E

I

I

-

I

-

I

-

D

-

G

-

I

-

N

-

L

-

K

A

A

A

K

E

D

T

T

E

N

I

L

T

N

G

K

L

V

R

R

P

E

D

E

L

-

L

-

A

-

R

-

H

-

G

-

I

V

A

G

Y

M

V

V

P

F

Q

Q

S

R

D

F

N

N

I

L

F

F

R

P

T

H

L

M

T

T

V

V

R

L

E

N

N

N

L

I

D

T

L

L

-

A

-

P

-

M

-

K

V

V

L

R

R

K

H

W

V

P

R

R

A

E

D

K

A

A

P

E

A

A

R

K

R

A

A

M

G

E

L

L

L

L

D

D

Q

K

F

V

P

G

V

-

L

L

A

K

D

D

K

K

L

A

G

H

D

A

K

Y

A

P

G

D

A

S

L

L

S

S

G

G

G

G

Q

Q

R

A

Q

Q

F

R

L

V

A

A

V

I

A

A

M

R

A

A

L

L

A

A

T

M

A

E

P

P

S

K

V

I

I

M

L

L

M

F

D
|
D

E

E

P

P

S

T

A

S

G

A

L

L

A

D

P

P

K

E

A

M

A

V

Q

G

E

E

V

V

L

L

E

S

H

V

A

M

R

K

A

Q

L

L

T

A

E

N

T

E

G

G

V

M

T

T

I

M

L

V

L

V

V

V

E

T

Q

H

N

E

V

M

K

G

Q

F

A

A

L

R

R

E

L

V

S

G

D

D

H

R

C

V

Y

L

I

F

L

M

A

D

D

G

G

G

R

Y

N

I

Q

I

I

E

D

E

G

G

P

K

A

P

A

E

D

D

L

L

L

F

D

D

D

R

P

P

binding phosphoaminophosphonic acid-adenylate ester: V18 (≠ I20), S38 (≠ N40), G39 (= G41), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)
binding magnesium ion: S43 (= S45), D163 (= D160)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

2olkA Abc protein artp in complex with adp-beta-s
32% identity, 87% coverage: 18:226/240 of query aligns to 16:229/242 of 2olkA

query
sites
2olkA

L

L

P

E

I
x
V

L

L

H

K

G

G

V

I

S

N

L

V

R

H

A

I

R

R

A

E

A

G

R

E

V

V

T

V

V

V

I

V

I

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

A

C

I

L

A

N

G

L

L

L

V

E

P

D

V

F

T

D

S

E

G

G

T

E

I

I

-

I

-

I

-

D

-

G

-

I

-

N

-

L

-

K

A

A

A

K

E

D

T

T

E

N

I

L

T

N

G

K

L

V

R

R

P

E

D

E

L

-

L

-

A

-

R

-

H

-

G

-

I

V

A

G

Y

M

V

V

P

F

Q

Q

S

R

D

F

N

N

I

L

F

F

R

P

T

H

L

M

T

T

V

V

R

L

E

N

N

N

L

I

D

T

L

L

-

A

-

P

-

M

-

K

V

V

L

R

R

K

H

W

V

P

R

R

A

E

D

K

A

A

P

E

A

A

R

K

R

A

A

M

G

E

L

L

L

L

D

D

Q

K

F

V

P

G

V

-

L

L

A

K

D

D

K

K

L

A

G

H

D

A

K

Y

A

P

G

D

A

S

L

L

S

S

G

G

G

G

Q

Q

R

A

Q

Q

F

R

L

V

A

A

V

I

A

A

M

R

A

A

L

L

A

A

T

M

A

E

P

P

S

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

S

T

A

S

G

A

L

L

A

D

P

P

K

E

A

M

A

V

Q

G

E

E

V

V

L

L

E

S

H

V

A

M

R

K

A

Q

L

L

T

A

E

N

T

E

G

G

V

M

T

T

I

M

L

V

L

V

V

V

E

T

Q

H

N

E

V

M

K

G

Q

F

A

A

L

R

R

E

L

V

S

G

D

D

H

R

C

V

Y

L

I

F

L

M

A

D

D

G

G

G

R

Y

N

I

Q

I

I

E

D

E

G

G

P

K

A

P

A

E

D

D

L

L

L

F

D

D

D

R

P

P

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: V18 (≠ I20), S38 (≠ N40), G39 (= G41), S40 (≠ A42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

Sites not aligning to the query:

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: 13

2oljA Abc protein artp in complex with adp/mg2+
32% identity, 87% coverage: 18:226/240 of query aligns to 16:229/242 of 2oljA

query
sites
2oljA

L

L

P

E

I
x
V

L

L

H

K

G

G

V

I

S

N

L

V

R

H

A

I

R

R

A

E

A

G

R

E

V

V

T

V

V

V

I

V

I

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

A

C

I

L

A

N

G

L

L

L

V

E

P

D

V

F

T

D

S

E

G

G

T

E

I

I

-

I

-

I

-

D

-

G

-

I

-

N

-

L

-

K

A

A

A

K

E

D

T

T

E

N

I

L

T

N

G

K

L

V

R

R

P

E

D

E

L

-

L

-

A

-

R

-

H

-

G

-

I

V

A

G

Y

M

V

V

P

F

Q

Q

S

R

D

F

N

N

I

L

F

F

R

P

T

H

L

M

T

T

V

V

R

L

E

N

N

N

L

I

D

T

L

L

-

A

-

P

-

M

-

K

V

V

L

R

R

K

H

W

V

P

R

R

A

E

D

K

A

A

P

E

A

A

R

K

R

A

A

M

G

E

L

L

L

L

D

D

Q

K

F

V

P

G

V

-

L

L

A

K

D

D

K

K

L

A

G

H

D

A

K

Y

A

P

G

D

A

S

L

L

S

S

G

G

G

G

Q

Q

R

A

Q

Q

F

R

L

V

A

A

V

I

A

A

M

R

A

A

L

L

A

A

T

M

A

E

P

P

S

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

S

T

A

S

G

A

L

L

A

D

P

P

K

E

A

M

A

V

Q

G

E

E

V

V

L

L

E

S

H

V

A

M

R

K

A

Q

L

L

T

A

E

N

T

E

G

G

V

M

T

T

I

M

L

V

L

V

V

V

E

T

Q

H

N

E

V

M

K

G

Q

F

A

A

L

R

R

E

L

V

S

G

D

D

H

R

C

V

Y

L

I

F

L

M

A

D

D

G

G

G

R

Y

N

I

Q

I

I

E

D

E

G

G

P

K

A

P

A

E

D

D

L

L

L

F

D

D

D

R

P

P

binding adenosine-5'-diphosphate: V18 (≠ I20), S38 (≠ N40), G39 (= G41), S40 (≠ A42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13

P34358 ABC transporter ced-7; Cell death protein 7 from Caenorhabditis elegans (see 2 papers)
37% identity, 79% coverage: 23:212/240 of query aligns to 565:754/1704 of P34358

query
sites
P34358

G

G

V

L

S

S

L

L

R

R

A

A

R

V

A

R

A

G

R

Q

V

C

T

S

V

I

I

L

I

L

G

G

P

H

N

N

G

G

A

A

G

G

K
|
K

S

S

T

T

L

T

I

F

K

S

A

S

I

I

A

A

G

G

L

I

V

I

P

R

V

P

T

T

S

N

G

G

T

R

I

I

A

T

A

I

E

C

T

G

E

Y

I

D

T

V

G

G

L

N

R

E

P

P

D

G

L

E

L

T

A

R

R

R

H

H

G

-

I

I

A

G

Y

M

V

C

P

P

Q

Q

S

Y

D

N

N

P

I

L

F

Y

R

D

T

Q

L

L

T

T

V

V

R

S

E
|
E

N

H

L

L

D

K

L

L

V

V

-

Y

-

G

L

L

R

K

H

G

V

A

R

R

A

E

D

K

A

D

P

F

A

K

R

Q

R

D

A

M

G

K

L

R

L

L

D

L

Q

S

F

D

P

V

V

K

L

L

A

D

D

F

K

K

L

E

G

N

D

E

K

K

A

A

G

V

A

N

L

L

S

S

G

G

G

G

Q

M

R

K

Q

R

F

K

L

L

A

C

V

V

A

C

M

M

A

A

L

L

A

I

T

G

A

D

P

S

S

E

V

V

I

V

L

L

M

L

D

D

E

E

P

P

S

T

A

A

G

G

L

M

A

D

P

P

K

G

A

A

A

R

Q

Q

E

D

V

V

-

Q

-

K

-

L

L

V

E

E

H

R

A

E

R

K

A

A

L

-

T

-

E

-

T

-

G

N

V

R

T

T

I

I

L

L

L

L

V

T

E

T

Q

H

N

Y

V

M

K

D

Q

E

A

A

L

E

R

R

L

L

S

G

D

D

H

W

C

V

Y

F

I

I

L

M

A

S

D

H

G

G

R

K

K586 (= K44) mutation to R: Cell corpses not engulfed.
E639 (= E98) mutation to G: Cell corpses not engulfed.

Sites not aligning to the query:

126 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1012 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1045 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1417 K→R: Some cell corpses not engulfed.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
32% identity, 93% coverage: 5:226/240 of query aligns to 3:227/241 of 4u00A

query
sites
4u00A

L

I

T

R

I

I

T

R

D

N

L

L

V

H

A

K

G

W

Y
x
F

R

G

P

P

G

-

L

L

P

H

I
x
V

L

L

H

K

G

G

V

I

S

H

L

L

R

E

A

V

R

A

A

P

A

G

R

E

V

K

T

L

V

V

I

I

I

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

R

A

T

I

I

A

N

G

R

L

L

V

E

P

D

V

F

T

Q

S

E

G

G

T

E

I

V

A

V

A

V

E

D

-

G

T

L

E

S

I

V

T

K

G

D

L

D

R

R

P

A

D

L

L

R

L

E

A

I

R

R

H

R

G

E

I

V

A

G

Y

M

V

V

P

F

Q

Q

S

Q

D

F

N

N

I

L

F

F

R

P

T

H

L

M

T

T

V

V

R

L

E

E

N

N

L

V

D

T

L

L

-

A

-

P

-

M

-

R

V

V

L

R

R

R

H

W

V

P

R

R

A

E

D

K

A

A

P

E

A

K

R

K

R

A

A

L

G

E

L

L

L

L

D

E

Q

R

F

V

P

G

V

I

L

L

A

-

D

D

K

Q

L

A

G

R

D

K

K

Y

A

P

G

A

A

Q

L

L

S

S

G

G

G

G

Q

Q

R

Q

Q

Q

F

R

L

V

A

A

V

I

A

A

M

R

A

A

L

L

A

A

T

M

A

E

P

P

S

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

S

T

A

S

G

A

L

L

A

D

P

P

K

E

A

M

A

V

Q

G

E

E

V

V

L

L

E

D

H

V

A

M

R

R

A

D

L

L

T

A

E

Q

T

G

G

G

V

M

T

T

I

M

L

V

L

V

V

V

E

T

Q

H

N

E

V

M

K

G

Q

F

A

A

L

R

R

E

L

V

S

A

D

D

H

R

C

V

Y

V

I

F

L

M

A

D

D

G

G

G

R

Q

N

I

Q

V

I

E

D

E

G

G

P

R

A

P

A

E

D

E

L

I

L

F

D

T

D

R

P

P

binding adenosine-5'-diphosphate: F12 (≠ Y14), V17 (≠ I20), S37 (≠ N40), G38 (= G41), S39 (≠ A42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46)

3d31A Modbc from methanosarcina acetivorans (see paper)
29% identity, 91% coverage: 21:238/240 of query aligns to 16:233/348 of 3d31A

query
sites
3d31A

L

L

H

D

G

N

V

L

S

S

L

L

R

K

A

V

R

E

A

S

A

G

R

E

V

Y

T

F

V

V

I

I

I

L

G

G

P

P

N

T

G

G

A

A

G

G

K

K

S

T

T

L

L

F

I

L

K

E

A

L

I

I

A

A

G

G

L

F

V

H

P

V

V

P

T

D

S

S

G

G

T

R

I

I

A

L

A

L

E

D

-

G

T

K

E

D

I

V

T

T

G

D

L

L

R

S

P

P

D

E

L

-

L

-

A

-

R

K

H

H

G

D

I

I

A

A

Y

F

V

V

P

Y

Q

Q

S

N

D

Y

N

S

I

L

F

F

R

P

T

H

L

M

T

N

V

V

R

K

E

K

N

N

L

L

D

E

L

F

V

G

L

M

R

R

H

M

V

K

R

K

A

I

D

K

A

D

P

P

A

K

R

R

R

V

-

L

-

D

-

T

-

A

-

R

-

D

-

L

-

K

-

I

A

E

G

H

L

L

L

L

D

D

Q

R

F

N

P

P

V

L

L

-

A

-

D

-

K

-

L

-

G

-

D

-

K

-

A

-

G

-

A

T

L

L

S

S

G

G

G

G

Q

E

R

Q

Q

Q

F

R

L

V

A

A

V

L

A

A

M

R

A

A

L

L

A

V

T

T

A

N

P

P

S

K

V

I

I

L

L

L

M

L

D

D

E

E

P

P

S

L

A

S

G

A

L

L

A

D

P

P

K

R

A

T

A

Q

Q

E

E

N

V

A

L

R

E

E

H

M

A

L

R

S

A

V

L

L

-

H

T

K

E

K

T

N

G

K

V

L

T

T

I

V

L

L

L

H

V

I

E

T

Q

H

N

D

V

Q

K

T

Q

E

A

A

L

R

R

I

L

M

S

A

D

D

H

R

C

I

Y

A

I

V

L

V

A

M

D

D

G

G

R

K

N

L

Q

I

I

Q

D

V

G

G

P

K

A

P

A

E

D

E

L

I

L

F

D

E

D

K

P

P

V

V

V

E

G

G

Q

R

I

V

-

A

-

S

Y

F

L

V

G

G

G

F

K

E

R

N

V

V

Sites not aligning to the query:

binding tungstate(vi)ion: 286, 320, 323, 340, 341, 342

Q5SSE9 ATP-binding cassette sub-family A member 13; EC 7.6.2.- from Mus musculus (Mouse) (see paper)
32% identity, 80% coverage: 32:222/240 of query aligns to 3837:4029/5034 of Q5SSE9

query
sites
Q5SSE9

R

Q

V

I

T

T

V

A

I

L

I

L

G

G

P

T

N

N

G

G

A

A

G

G

K
|
K

S

T

T

T

L

I

I

I

K

S

A

M

I

L

A

M

G

G

L

L

V

F

P

P

V

P

T

T

S

S

G

G

T

T

I

I

A

T

A

I

E

N

T

G

E

K

I

-

T

-

G

N

L

L

R

Q

P

T

D

D

L

L

-

S

L

K

A

V

R

R

H

E

G

E

I

L

A

G

Y

V

V

C

P

P

Q

Q

S

Q

D

D

N

V

I

L

F

L

R

D

T

N

L

L

T

T

V

V

R

R

E

E

N

H

L

L

D

-

L

M

V

L

L

F

R

A

H

S

V

I

R

K

A

A

-

P

-

W

-

W

-

T

-

T

-

K

D

E

A

L

P

Q

A

Q

R

Q

R

V

A

N

G

K

L

T

L

L

D

D

Q

E

F

V

P

E

V

-

L

L

A

T

D

Q

K

H

L

Q

G

H

D

K

K

P

A

A

G

G

A

V

L

L

S

S

G

G

G

G

Q

M

R

K

Q

R

F

K

L

L

A

S

V

I

A

G

M

I

A

A

L

F

A

M

T

G

A

M

P

S

S

K

V

T

I

V

L

V

M

L

D

D

E

E

P

P

S

S

A

S

G

G

L

V

A

D

P

P

K

C

A

S

A

R

Q

R

E

S

V

L

L

W

E

D

H

I

A

L

R

L

A

K

L

Y

T

R

E

E

T

-

G

G

V

R

T

T

I

I

L

I

L

F

V

T

E
x
T

Q

H

N

H

V

L

K

D

Q

E

A

A

L

E

R

M

L

L

S

S

D

D

H

H

C

V

Y

A

I

V

L

L

A

Q

D

Q

G

G

R

R

N

L

Q

R

I

C

D

Y

G

A

P

P

A

P

A

A

D

D

L

L

K3849 (= K44) mutation to M: Does not affect intracellular vesicle localization. Affects cholesterol internalization.
T3999 (≠ E192) mutation to A: Does not affect protein expression. Affects intracellular vesicles localization. Impairs intracellular cholesterol accumulation in the vesicle.

Sites not aligning to the query:

3577 H→P: Does not affect protein expression. Decreases intracellular cholesterol accumulation in the vesicle.
4735 K→M: Does not affect intracellular vesicle localization.Affects cholesterol internalization.
4818 R→C: Does not affect protein expression. Decreases intracellular cholesterol accumulation in the vesicle.

Query Sequence

>3608586 Dshi_1980 ABC transporter related (RefSeq)
MADALTITDLVAGYRPGLPILHGVSLRARAARVTVIIGPNGAGKSTLIKAIAGLVPVTSG
TIAAETEITGLRPDLLARHGIAYVPQSDNIFRTLTVRENLDLVLRHVRADAPARRAGLLD
QFPVLADKLGDKAGALSGGQRQFLAVAMALATAPSVILMDEPSAGLAPKAAQEVLEHARA
LTETGVTILLVEQNVKQALRLSDHCYILADGRNQIDGPAADLLDDPVVGQIYLGGKRVAS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory