SitesBLAST
Comparing 3608643 FitnessBrowser__Dino:3608643 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2ejvA Crystal structure of threonine 3-dehydrogenase complexed with NAD+
30% identity, 97% coverage: 12:327/327 of query aligns to 13:341/343 of 2ejvA
- active site: C38 (= C37), G39 (= G38), T40 (≠ S39), H43 (= H42), H63 (= H59), E64 (= E60), C93 (= C83), C96 (= C86), C99 (= C89), C107 (= C97), Q111 (= Q101), P149 (= P140), A153 (≠ S144), K336 (= K322)
- binding nicotinamide-adenine-dinucleotide: G172 (= G167), G174 (= G169), P175 (≠ A170), I176 (= I171), S195 (≠ V190), D196 (≠ E191), P197 (= P192), R201 (= R196), F238 (≠ G225), S239 (≠ V226), N241 (≠ Y228), A244 (≠ T231), L261 (≠ I248), G262 (= G249), I263 (≠ L250)
- binding zinc ion: C38 (= C37), H63 (= H59), E64 (= E60), C96 (= C86), C99 (= C89), C107 (= C97)
2dq4A Crystal structure of threonine 3-dehydrogenase
30% identity, 97% coverage: 12:327/327 of query aligns to 13:341/343 of 2dq4A
- active site: C38 (= C37), G39 (= G38), T40 (≠ S39), H43 (= H42), H63 (= H59), E64 (= E60), C93 (= C83), C96 (= C86), C99 (= C89), C107 (= C97), Q111 (= Q101), P149 (= P140), A153 (≠ S144), K336 (= K322)
- binding zinc ion: C38 (= C37), H63 (= H59), E64 (= E60), C93 (= C83), C96 (= C86), C107 (= C97)
Q5SKS4 L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
30% identity, 97% coverage: 12:327/327 of query aligns to 13:341/343 of Q5SKS4
- C38 (= C37) binding
- H63 (= H59) binding
- E64 (= E60) binding
- C93 (= C83) binding
- C96 (= C86) binding
- C99 (= C89) binding
- C107 (= C97) binding
- I176 (= I171) binding
- D196 (≠ E191) binding
- R201 (= R196) binding
- LGI 261:263 (≠ IGL 248:250) binding
- IAG 286:288 (≠ TY- 272:273) binding
P07846 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Ovis aries (Sheep) (see paper)
29% identity, 83% coverage: 3:275/327 of query aligns to 9:297/354 of P07846
- C43 (= C37) binding
- Y49 (≠ A43) binding
- H67 (= H59) binding
- E68 (= E60) binding
- E153 (= E139) binding
- R296 (≠ T274) binding
- Y297 (= Y275) binding
1pl6A Human sdh/nadh/inhibitor complex (see paper)
28% identity, 99% coverage: 3:326/327 of query aligns to 10:348/356 of 1pl6A
- active site: C44 (= C37), G45 (= G38), S46 (= S39), H49 (= H42), H69 (= H59), E70 (= E60), R99 (≠ C83), D102 (≠ C86), C105 (= C89), S113 (≠ C97), F117 (≠ Q101), P156 (= P140), G160 (≠ S144), K344 (= K322)
- binding 4-[2-(hydroxymethyl)pyrimidin-4-yl]-n,n-dimethylpiperazine-1-sulfonamide: C44 (= C37), S46 (= S39), I56 (≠ L45), F59 (≠ D48), H69 (= H59), E155 (= E139), L274 (= L250), F297 (≠ Y273)
- binding nicotinamide-adenine-dinucleotide: G181 (= G169), P182 (≠ A170), I183 (= I171), D203 (≠ E191), L204 (≠ P192), R208 (= R196), C249 (≠ G225), T250 (≠ V226), V272 (≠ I248), G273 (= G249), L274 (= L250), F297 (≠ Y273), R298 (≠ T274)
- binding zinc ion: C44 (= C37), H69 (= H59)
3qe3A Sheep liver sorbitol dehydrogenase (see paper)
29% identity, 83% coverage: 3:275/327 of query aligns to 5:294/351 of 3qe3A
- active site: C39 (= C37), G40 (= G38), S41 (= S39), H44 (= H42), H64 (= H59), E65 (= E60), R94 (≠ C83), D97 (≠ C86), C100 (= C89), S108 (≠ C97), F112 (≠ Q101), P151 (= P140), G155 (≠ S144)
- binding glycerol: Y45 (= Y44), F54 (≠ D49), T116 (≠ M105), R293 (≠ T274)
- binding zinc ion: C39 (= C37), H64 (= H59), E65 (= E60)
Sites not aligning to the query:
2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
31% identity, 97% coverage: 10:326/327 of query aligns to 15:346/346 of 2dfvA
- active site: C40 (= C37), G41 (= G38), T42 (≠ S39), H45 (= H42), H65 (= H59), E66 (= E60), C95 (= C83), C98 (= C86), C101 (= C89), C109 (= C97), K113 (≠ Q101), P151 (= P140), A155 (= A147), K340 (≠ A320)
- binding nicotinamide-adenine-dinucleotide: G175 (= G169), P176 (≠ A170), L177 (≠ I171), E197 (= E191), P198 (= P192), R202 (= R196), F241 (≠ A234), S242 (≠ A235), A244 (= A237), L264 (≠ I248), G265 (= G249), L266 (= L250), I289 (vs. gap), T290 (= T272)
- binding zinc ion: C95 (= C83), C101 (= C89), C109 (= C97)
Q00796 Sorbitol dehydrogenase; SDH; (R,R)-butanediol dehydrogenase; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Ribitol dehydrogenase; RDH; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.4; EC 1.1.1.14; EC 1.1.1.56; EC 1.1.1.9 from Homo sapiens (Human) (see 10 papers)
28% identity, 99% coverage: 3:326/327 of query aligns to 11:349/357 of Q00796
- C45 (= C37) binding
- H70 (= H59) binding
- E71 (= E60) binding
- R110 (= R93) to P: in SORDD; results in protein aggregation
- H135 (≠ M118) to R: in SORDD; results in protein aggregation
- A153 (= A136) to D: in SORDD; uncertain significance; results in protein aggregation; dbSNP:rs145813597
- I184 (= I171) binding
- D204 (≠ E191) binding
- R209 (= R196) binding
- Q239 (vs. gap) to L: in dbSNP:rs1042079
- N269 (≠ L244) to T: in dbSNP:rs930337
- VGL 273:275 (≠ IGL 248:250) binding
- VFR 297:299 (≠ TYT 272:274) binding
- V322 (≠ W297) to I: in SORDD; uncertain significance; dbSNP:rs149975952
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
31% identity, 97% coverage: 10:326/327 of query aligns to 17:348/348 of O58389
- C42 (= C37) binding
- T44 (≠ S39) mutation to A: Total loss of enzymatic activity.
- H67 (= H59) binding
- E68 (= E60) binding
- C97 (= C83) binding
- C100 (= C86) binding
- C103 (= C89) binding
- C111 (= C97) binding
- E152 (= E139) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
- L179 (≠ I171) binding
- E199 (= E191) binding ; mutation to A: Large decrease in affinity for NAD(+).
- R204 (= R196) binding ; mutation to A: Large decrease in affinity for NAD(+).
- LGL 266:268 (≠ IGL 248:250) binding
- IT 291:292 (≠ -T 272) binding
- R294 (≠ T274) mutation to A: 4000-fold decrease in catalytic efficiency.
7y9pA Xylitol dehydrogenase s96c/s99c/y102c mutant(thermostabilized form) from pichia stipitis (see paper)
28% identity, 88% coverage: 3:289/327 of query aligns to 5:309/357 of 7y9pA
P27867 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Rattus norvegicus (Rat) (see paper)
28% identity, 96% coverage: 3:316/327 of query aligns to 11:341/357 of P27867
1e3jA Ketose reductase (sorbitol dehydrogenase) from silverleaf whitefly (see paper)
29% identity, 93% coverage: 12:314/327 of query aligns to 13:333/348 of 1e3jA
- active site: C38 (= C37), G39 (= G38), S40 (= S39), H43 (= H42), H63 (= H59), E64 (= E60), C93 (= C83), C96 (= C86), C99 (= C89), C107 (= C97), T111 (≠ Q101), P150 (= P140), G154 (≠ S144)
- binding phosphate ion: A174 (≠ G168), A196 (vs. gap), R197 (vs. gap), S198 (vs. gap), R201 (vs. gap)
- binding zinc ion: C38 (= C37), H63 (= H59), E64 (= E60), C93 (= C83), C96 (= C86), C99 (= C89), C107 (= C97)
Sites not aligning to the query:
4ej6A Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021
28% identity, 83% coverage: 1:273/327 of query aligns to 4:290/343 of 4ej6A
- active site: C40 (= C37), G41 (= G38), T42 (≠ S39), H45 (= H42), H61 (= H59), E62 (= E60), C91 (= C83), C94 (= C86), C97 (= C89), C105 (= C97), R109 (≠ Q101), P147 (= P140), C151 (≠ S144)
- binding zinc ion: C91 (= C83), C94 (= C86), C97 (= C89), C105 (= C97)
Sites not aligning to the query:
4ejmA Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021 bound to NADP
28% identity, 83% coverage: 1:273/327 of query aligns to 4:290/342 of 4ejmA
- active site: C40 (= C37), G41 (= G38), T42 (≠ S39), H45 (= H42), H61 (= H59), E62 (= E60), C91 (= C83), C94 (= C86), C97 (= C89), C105 (= C97), R109 (≠ Q101), P147 (= P140), C151 (≠ S144)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G170 (= G167), G172 (= G169), V173 (≠ A170), I174 (= I171), T194 (≠ E191), R195 (≠ P192), Q196 (≠ N193), K199 (≠ R196), C240 (≠ G225), E245 (≠ A230), T246 (= T231), L263 (≠ I248), V265 (≠ L250)
- binding zinc ion: C91 (= C83), C94 (= C86), C97 (= C89), C105 (= C97)
Sites not aligning to the query:
4ilkA Crystal structure of short chain alcohol dehydrogenase (rspb) from e. Coli cft073 (efi target efi-506413) complexed with cofactor nadh
27% identity, 83% coverage: 1:272/327 of query aligns to 4:282/337 of 4ilkA
- active site: C40 (= C37), G41 (= G38), S42 (= S39), H45 (= H42), H59 (= H59), E60 (= E60), C89 (= C83), C92 (= C86), C95 (= C89), C103 (= C97), A107 (≠ Q101), P145 (= P140), A149 (≠ S144)
- binding manganese (ii) ion: C40 (= C37), H59 (= H59), E60 (= E60), E144 (= E139)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G168 (= G167), G170 (= G169), P171 (≠ A170), I172 (= I171), D193 (≠ E191), R194 (≠ P192), R198 (= R196), N213 (≠ T210), A235 (≠ G225), A236 (≠ V226), C237 (≠ G227), I241 (≠ T231), M258 (≠ I248), F260 (≠ L250)
- binding zinc ion: C89 (= C83), C92 (= C86), C95 (= C89), C103 (= C97)
Sites not aligning to the query:
3gfbA L-threonine dehydrogenase (tktdh) from the hyperthermophilic archaeon thermococcus kodakaraensis (see paper)
31% identity, 94% coverage: 10:317/327 of query aligns to 15:337/347 of 3gfbA
- active site: C40 (= C37), G41 (= G38), T42 (≠ S39), H45 (= H42), H65 (= H59), E66 (= E60), C95 (= C83), C98 (= C86), C101 (= C89), C109 (= C97), K113 (≠ Q101), P151 (= P140), A155 (= A147)
- binding nicotinamide-adenine-dinucleotide: G173 (= G167), G175 (= G169), P176 (≠ A170), L177 (≠ I171), S196 (≠ V190), E197 (= E191), P198 (= P192), R202 (= R196), F241 (≠ A234), S242 (≠ A235), A244 (= A237), L264 (≠ I248), G265 (= G249), L266 (= L250), I289 (vs. gap), T290 (= T272)
Sites not aligning to the query:
Q5JI69 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
31% identity, 94% coverage: 10:317/327 of query aligns to 17:339/350 of Q5JI69
P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
28% identity, 83% coverage: 1:273/327 of query aligns to 1:287/341 of P07913
- C38 (= C37) mutation to D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+).; mutation to S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+).
5vm2A Crystal structure of eck1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from escherichia coli
27% identity, 83% coverage: 20:292/327 of query aligns to 22:309/347 of 5vm2A
- active site: C39 (= C37), G40 (= G38), S41 (= S39), H44 (= H42), H65 (= H59), E66 (= E60), C95 (= C83), C98 (= C86), C101 (= C89), C109 (= C97), D113 (≠ Q101), P153 (= P140), G157 (≠ S144)
- binding magnesium ion: H65 (= H59), E66 (= E60), E152 (= E139)
- binding zinc ion: C95 (= C83), C98 (= C86), C101 (= C89), C109 (= C97)
Sites not aligning to the query:
A2QAC0 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Aspergillus niger (strain ATCC MYA-4892 / CBS 513.88 / FGSC A1513) (see 2 papers)
29% identity, 77% coverage: 25:275/327 of query aligns to 43:320/386 of A2QAC0
- M70 (≠ L55) mutation to F: Abolishes enzyme activity.
- DI 213:214 (≠ EP 191:192) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-359.
- Y318 (= Y273) mutation to F: Increases affinity for D-sorbitol.
Sites not aligning to the query:
- 359 A→T: Alters cofactor specificity from NAD to NADP; when associated with 213-SR-214.
Query Sequence
>3608643 FitnessBrowser__Dino:3608643
MKALVYTGVAQLAFRDVPEPVPAAGDHLIRIDSVGICGSDMHAYLGHDDRRPAPLILGHE
GAGVIIGGPRDGERVTINPLVTCGTCPACVSGRDNLCATRQIISMPPRDGAFAQYVAMPA
RNLVTVPDDVPLEKAALAEPVAVSWHAVRLGLASMADARRDSALVIGGGAIGVAAAISLQ
AQGVADVTLVEPNAMRREYLARDANYTIATPEQVAGRVFDITVDGVGYDATRAAASAATR
PGGLLLHIGLGGGSAGLDIRRITLQEITVIGTYTYTAQDFRDTCAAMFDGRLGGLDWTES
RPLSAGADAFADIRAGRVPAPKIILKP
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory