SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3608769 FitnessBrowser__Dino:3608769 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
55% identity, 92% coverage: 8:254/269 of query aligns to 3:249/250 of 4hzdA

query
sites
4hzdA

L

L

K

D

A

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V

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P

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x
I

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G

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C

binding coenzyme a: H158 (= H163), G203 (= G208), A204 (= A209), A221 (= A226), A222 (= A227), T235 (= T240), A237 (= A242), I244 (≠ V249)
binding magnesium ion: S224 (= S229), A237 (= A242), G238 (= G243)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
55% identity, 95% coverage: 8:263/269 of query aligns to 4:258/261 of 6wyeA

query
sites
6wyeA

L

L

K

N

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W

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-

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D

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Q

binding (2S)-2-hydroxybutanedioic acid: D93 (= D97), P94 (= P98), D158 (= D162), H159 (= H163), G185 (= G189), R193 (= R197), H194 (= H198)

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
54% identity, 96% coverage: 7:263/269 of query aligns to 2:258/262 of 1t3dA

query
sites
1t3dA

S

S

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H

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C

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Q

binding cysteine: D92 (= D97), P93 (= P98), D157 (= D162), G184 (= G189), R192 (= R197), H193 (= H198)

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
56% identity, 93% coverage: 14:263/269 of query aligns to 5:254/258 of 8i04A

query
sites
8i04A

V

V

W

W

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E

E

A

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L

-

A

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D

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C

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H

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H

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A

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P

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L

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V

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D

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P

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A

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E

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G

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V

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M

I

I

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G

A

A

G

G

A

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V

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L

G

G

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I

A

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A

A

A

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Q

binding serine: D88 (= D97), P89 (= P98), D153 (= D162), H154 (= H163), G180 (= G189), R188 (= R197), H189 (= H198)

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
57% identity, 88% coverage: 14:249/269 of query aligns to 8:243/243 of 7ra4A

query
sites
7ra4A

V

L

W

W

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H

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A

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F

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D

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I

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I

A

G

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N

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T

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L

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D

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H
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H

P

P

K

K

I

I

G

G

D

D

G

G

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V

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M

I

I

G

G

A

A

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N

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I

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L

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G

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N

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I

A

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A

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L

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D

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V

P

P

P

P

C

S

K

I

T

T

V

V

A

V

G

G

V

V

P

P

A

A

K

K

I

P

V

V

binding serine: D91 (= D97), P92 (= P98), D156 (= D162), H157 (= H163), R191 (= R197), H192 (= H198)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
53% identity, 98% coverage: 7:269/269 of query aligns to 5:267/272 of 3gvdI

query
sites
3gvdI

S

S

L

S

K

E

A

E

V

L

D

E

P

Q

V

V

W

W

H

S

R

N

I

I

G

K

E

S

E

E

A

A

R

R

L

A

A

L

V

A

A

E

D

C

E

E

Q

P

L

M

L

L

G

A

G

S

L

F

V

F

H

H

S

A

S

T

I

L

L

L

H

K

H

H

A

E

T

N

F

L

E

G

A

S

A

A

L

L

A

S

Y

Y

R

I

I

L

T

A

L

N

K

K

L

L

S

A

S

N

P

P

E

I

M

M

S

P

A

A

Q

I

T

A

L

I

R

R

Q

E

L

V

V

V

D

E

D

E

A

A

F

Y

A

R

S

S

D

D

A

A

T

H

L

M

G

I

V

V

A

S

A

A

R

A

A

R

D

D

L

I

V

L

A

A

V

V

Y

R

E

L

R

R

D
|
D

P
|
P

A

A

C

V

H

D

R

K

L

Y

M

S

Q

T

P

P

L

L

L

L

Y

Y

F

L

K

K

G

G

F

F

Q

H

A

A

V

L

Q

Q

A

A

Y

Y

R

R

V

I

G

G

H

H

W

W

L

L

Y

W

S

A

Q

Q

G

D

R

R

H

K

D

A

L

L

A

A

Y

I

F

Y

V

L

Q

Q

M

N

R

Q

V

V

S

S

E

V

V

A

F

F

G

G

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

T

I

I

G

G

Q

C

G

G

I

I

M

M

I

L

D
|
D

H

H

A

A

H

T

S

G

I

I

V

V

I

I

G

G

E

E

T

T

A

A

V

V

V

V

G

E

D

N

N

D

V

V

S

S

M

I

L

L

H

Q

S

S

V

V

T

T

L

L

G
|
G

G
|
G

T

T

G

G

K

K

E

T

D

S

D

G

D

D

R
|
R

H

H

P

P

K

K

I

I

G

R

D

E

G

G

V

V

L

M

I

I

G

G

A

A

G

G

A

A

H

K

V

I

L

L

G

G

N

N

I

I

T

E

I

V

G

G

H

R

C

G

S

A

R

K

I

I

A

G

A

A

G

G

S

S

V

V

V

V

L

L

S

Q

D

S

V

V

P

P

P

A

C

H

K

T

T

T

V

A

A

A

G

G

V

V

P

P

A

A

K

R

I

I

V

V

G

G

E

K

A

P

G

E

C

S

A

D

Q

K

P

P

S

S

V

L

T

D

M

M

D

D

Q

Q

L

H

L

F

S

N

E

G

S

S

I

I

binding cysteine: D95 (= D97), P96 (= P98), D160 (= D162), G186 (= G188), G187 (= G189), R195 (= R197)

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
56% identity, 88% coverage: 14:251/269 of query aligns to 8:245/246 of 8i09A

query
sites
8i09A

V

V

W

W

H

K

R

N

I

I

G

K

E

A

E

E

A

A

R

R

L

-

A

A

V

L

A

A

D

D

-

C

E

E

Q

P

L

M

L

L

G

A

G

S

L

F

V

Y

H

H

S

A

S

T

I

L

L

L

H

K

H

H

A

E

T

N

F

L

E

G

A

S

A

A

L

L

A

S

Y

Y

R

M

I

L

T

A

L

N

K

K

L

L

S

A

S

S

P

P

E

I

M

M

S

P

A

A

Q

I

T

A

L

I

R

R

Q

E

L

V

V

V

D

E

D

E

A

A

F

Y

A

A

S

A

D

D

A

P

T

E

L

M

G

I

V

A

A

S

A

A

R

A

A

C

D

D

L

I

V

Q

A

A

V

V

Y

R

E

T

R

R

D
|
D

P

P

A

A

C

V

H

D

R

K

L

Y

M

S

Q

T

P

P

L

L

L

L

Y

Y

F

L

K

K

G

G

F

F

Q

H

A

A

V

L

Q

Q

A

A

Y

Y

R

R

V

I

G

G

H

H

W

W

L

L

Y

W

S

N

Q

K

G

G

R

R

H

R

D

A

L

L

A

A

Y

I

F

F

V

L

Q

Q

M

N

R

Q

V

V

S

S

E

V

V

S

F

F

G

Q

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

K

I

I

G

G

Q

R

G

G

I

I

M

M

I

L

D
|
D

H
|
H

A

A

H

T

S

G

I

I

V

V

I

V

G
|
G

E

E

T

T

A

A

V

V

V

I

G

E

D

D

N

D

V

V

S

S

M

I

L

L

H

Q

S

S

V

V

T

T

L

L

G

G

G
|
G

T
|
T

G

G

K

K

E

T

D

S

D

G

D

D

R
|
R

H
|
H

P

P

K

K

I

I

G

R

D

E

G

G

V

V

L

M

I

I

G
|
G

A

A

G

G

A

A

H

K

V

I

L

L

G

G

N

N

I

I

T

E

I

V

G

G

H

R

C

G

S

A

R
x
K

I
|
I

A
x
G

A

A

G

G

S

S

V

V

V

V

L
|
L

S

Q

D

P

V

V

P

P

P

P

C

H

K

T

T

T

V

A

A
|
A

G

G

V

V

P

P

A

A

K

R

I

I

V

V

G

G

E

K

binding cysteine: D91 (= D97), D156 (= D162), H157 (= H163), G164 (= G170), R191 (= R197), H192 (= H198)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (= G189), T184 (= T190), G202 (= G208), K218 (≠ R224), I219 (= I225), G220 (≠ A226), L226 (= L232), A236 (= A242)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
51% identity, 94% coverage: 14:266/269 of query aligns to 5:257/258 of 4h7oA

query
sites
4h7oA

V

V

W

W

H

Q

R

T

I

I

G

V

E

A

E

E

A

A

R

R

L

E

A

Q

V

A

A

E

D

Q

E

E

Q

P

L

M

L

L

G

A

G

S

L

F

V

Y

H

H

S

A

S

T

I

I

L

I

H

K

H

H

A

D

T

S

F

L

E

K

A

A

A

A

L

L

A

S

Y

Y

R

I

I

L

T

A

L

N

K

R

L

L

S

N

S
x
T

P

A

E
x
S

M

M

S

P

A

A

Q

M

T

A

L

V

R

R

Q

E

L

V

V

I

D

E

D

E

A

A

F

F

A

A

S

A

D

D

A

P

T

S

L

I

G

S

V

E

A

A

A

A

R

A

A

C

D

D

L

I

V

C

A

A

V

T

Y

V

E

N

R

R

D
|
D

P
|
P

A

A

C

V

H

S

R

M

L

Y

M

S

Q

M

P

P

L

L

L

L

Y

Y

F

L

K

K

G

G

F

Y

Q

H

A

A

V

L

Q

Q

A

G

Y

Y

R

R

V

V

G

A

H

N

W

W

L

L

Y

W

S

R

Q

Q

G

G

R

R

H

K

D

A

L

L

A

A

Y

T

F
x
Y

V

F

Q

Q

M

N

R
x
Q

V

I

S

S

E

V

V

A

F

C

G

Q

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

R

I

I

G

G

Q

R

G

G

I

I

M

M

I

L

D
|
D

H
|
H

A

A

H

T

S

G

I

I

V

V

I

I

G

G

E

E

T

T

A

A

V

V

V

V

G

E

D

D

N

D

V

V

S

S

M

I

L

L

H

Q

S

D

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

E

D

C

D

G

D

D

R
|
R

H
|
H

P

P

K

K

I

I

G

R

D

E

G

G

V

V

L

M

I

I

G

G

A

A

G

G

A

A

H

K

V

I

L

L

G

G

N

N

I

I

T

E

I

V

G

G

H

E

C

G

S

A

R

K

I

I

A

G

A

S

G

G

S

S

V

V

V

V

L

L

S

Q

D

A

V

V

P

P

P

P

C

H

K

T

T

T

V

V

A

A

G

G

V

V

P

P

A

A

K

R

I

I

V

V

G

G

E

R

A

P

G

Q

C

S

A

D

Q

K

P

P

S

S

V

L

T

D

M

M

D

D

Q

Q

L

Q

L

F

S

N

binding arginine: T51 (≠ S60), S53 (≠ E62), Y127 (≠ F136), Q131 (≠ R140)
binding cysteine: D88 (= D97), P89 (= P98), D153 (= D162), H154 (= H163), G180 (= G189), R188 (= R197), H189 (= H198)

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
56% identity, 88% coverage: 14:249/269 of query aligns to 9:244/244 of 8i06A

query
sites
8i06A

V

V

W

W

H

K

R

N

I

I

G

K

E

A

E

E

A

A

R

R

L

-

A

A

V

L

A

A

D

D

-

C

E

E

Q

P

L

M

L

L

G

A

G

S

L

F

V

Y

H

H

S

A

S

T

I

L

L

L

H

K

H

H

A

E

T

N

F

L

E

G

A

S

A

A

L

L

A

S

Y

Y

R

M

I

L

T

A

L

N

K

K

L

L

S

A

S

S

P

P

E

I

M

M

S

P

A

A

Q

I

T

A

L

I

R

R

Q

E

L

V

V

V

D

E

D

E

A

A

F

Y

A

A

S

A

D

D

A

P

T

E

L

M

G

I

V

A

A

S

A

A

R

A

A

C

D

D

L

I

V

Q

A

A

V

V

Y

R

E

T

R

R

D

D

P
|
P

A

A

C

V

H

D

R

K

L

Y

M

S

Q

T

P

P

L

L

L

L

Y

Y

F

L

K

K

G

G

F

F

Q

H

A

A

V

L

Q

Q

A

A

Y

Y

R

R

V

I

G

G

H

H

W

W

L

L

Y

W

S

N

Q

K

G

G

R

R

H

R

D

A

L

L

A

A

Y

I

F

F

V

L

Q

Q

M

N

R

Q

V

V

S

S

E

V

V

S

F

F

G

Q

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

K

I

I

G

G

Q

R

G

G

I

I

M

M

I

L

D

D

H
|
H

A

A

H

T

S

G

I

I

V

V

I

V

G

G

E

E

T

T

A

A

V

V

V

I

G

E

D

D

N

D

V

V

S

S

M

I

L
|
L

H
x
Q

S

S

V

V

T

T

L

L

G

G

G

G

T

T

G

G

K

K

E

T

D

S

D

G

D

D

R

R

H

H

P

P

K

K

I

I

G

R

D

E

G

G

V

V

L

M

I

I

G
|
G

A
|
A

G

G

A

A

H

K

V

I

L

L

G

G

N

N

I

I

T

E

I

V

G

G

H

R

C

G

S

A

R

K

I

I

A
x
G

A
|
A

G

G

S

S

V

V

V

V

L

L

S

Q

D

P

V

V

P

P

P

P

C

H

K

T

T
|
T

V

A

A
|
A

G

G

V

V

P

P

A

A

K

R

I

I

V

V

binding coenzyme a: H158 (= H163), L177 (= L182), Q178 (≠ H183), G203 (= G208), A204 (= A209), G221 (≠ A226), A222 (= A227), T235 (= T240), A237 (= A242)
binding cysteine: P93 (= P98), H158 (= H163)

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
49% identity, 94% coverage: 14:265/269 of query aligns to 5:256/257 of 1ssqD

query
sites
1ssqD

V

V

W

W

H

Q

R

H

I

I

G

R

E

Q

E

E

A

A

R

K

L

E

A

L

V

A

A

E

D

N

E

E

Q

P

L

M

L

L

G

A

G

S

L

F

V

F

H

H

S

S

S

T

I

I

L

L

H

K

H

H

A

Q

T

N

F

L

E

G

A

G

A

A

L

L

A

S

Y

Y

R

L

I

L

T

A

L

N

K

K

L

L

S

A

S

N

P

P

E

I

M

M

S

P

A

A

Q

I

T

S

L

L

R

R

Q

E

L

I

V

I

D

E

D

E

A

A

F

Y

A

Q

S

S

D

N

A

P

T

S

L

I

G

I

V

D

A

C

A

A

R

A

A

C

D

D

L

I

V

Q

A

A

V

V

Y

R

E

H

R

R

D
|
D

P

P

A

A

C

V

H

E

R

L

L

W

M

S

Q

T

P

P

L

L

L

L

Y

Y

F

L

K

K

G

G

F

F

Q

H

A

A

V

I

Q

Q

A

S

Y

Y

R

R

V

I

G

T

H

H

W

Y

L

L

Y

W

S

N

Q

Q

G

N

R

R

H

K

D

S

L

L

A

A

Y

L

F

Y

V

L

Q

Q

M

N

R

Q

V

I

S

S

E

V

V

A

F

F

G

D

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

K

I

I

G

G

Q

H

G

G

I

I

M

M

I

F

D
|
D

H
|
H

A

A

H

T

S

G

I

I

V

V

I

V

G

G

E

E

T

T

A

S

V

V

V

I

G

E

D

N

N

D

V

V

S

S

M

I

L

L

H

Q

S

G

V

V

T

T

L

L

G

G

G

G

T

T

G

G

K

K

E

E

D

S

D

G

D

D

R

R

H

H

P

P

K

K

I

V

G

R

D

E

G

G

V

V

L

M

I

I

G

G

A

A

G

G

A

A

H

K

V

I

L

L

G

G

N

N

I

I

T

E

I

V

G

G

H

K

C

Y

S

A

R

K

I

I

A

G

A

A

G

N

S

S

V

V

V

V

L

L

S

N

D

P

V

V

P

P

P

E

C

Y

K

A

T

T

V

A

A

A

G

G

V

V

P

P

A

A

K

R

I

I

V

V

G

S

E

Q

A

D

G

K

C

A

A

A

Q

K

P

P

S

A

V

F

T

D

M

M

D

N

Q

Q

L

Y

L

F

binding cysteine: D88 (= D97), D153 (= D162), H154 (= H163)

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
53% identity, 88% coverage: 14:249/269 of query aligns to 8:243/243 of 4n69A

query
sites
4n69A

V

V

W

W

H

G

R

Q

I

I

G

K

E

A

E

E

A

A

R

R

L

R

A

D

V

A

A

E

D

S

E

E

Q

P

L

A

L

L

G

A

G

S

L

Y

V

L

H

Y

S

S

S

T

I

I

L

L

H

S

H

H

A

S

T

S

F

L

E

E

A

R

A

S

L

L

A

S

Y

F

R

H

I

L

T

G

L

N

K

K

L

L

S

C

S

S

P

S

E

T

M

L

S

L

A

S

Q

T

T

L

L

L

R

Y

Q

D

L

L

V

F

D

L

D

N

A

A

F

F

A

S

S

S

D

D

A

P

T

S

L

L

G

R

V

S

A

A

A

A

R

V

A

A

D

D

L

L

V

R

A

A

V

A

Y

R

E

E

R

R

D

D

P

P

A

A

C

C

H

V

R

S

L

Y

M

S

Q

H

P

C

L

L

L

L

Y

N

F

Y

K

K

G

G

F

F

Q

L

A

A

V

C

Q

Q

A

A

Y

H

R

R

V

V

G

A

H

H

W

L

L

L

Y

W

S

R

Q

Q

G

S

R

R

H

R

D

P

L

L

A

A

Y

L

F

A

V

L

Q

H

M

S

R

R

V

I

S

A

E

N

V

V

F

F

G

A

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

R

I

I

G

G

Q

K

G

G

I

I

M

L

I

F

D

D

H

H

A

A

H

T

S

G

I

V

V

V

I

V

G

G

E

E

T

T

A

A

V

V

V

I

G

G

D

N

N

N

V

V

S

S

M

I

L

L

H

H

S

H

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

V

D

G

D

G

D

D

R
|
R

H
|
H

P

P

K

K

I

I

G

G

D

D

G

G

V

V

L

L

I

I

G

G

A

A

G

G

A

A

H

T

V

I

L

L

G

G

N

N

I

I

T

K

I

I

G

G

H

E

C

G

S

A

R

K

I

V

A

G

A

A

G

G

S

S

V

V

V

V

L

L

S

I

D

D

V

V

P

P

P

P

C

R

K

T

T

T

V

A

A

V

G

G

V

N

P

P

A

A

K

R

I

L

V

V

binding serine: G183 (= G189), R191 (= R197), H192 (= H198)

1sstA Serine acetyltransferase- complex with coa (see paper)
48% identity, 88% coverage: 14:249/269 of query aligns to 5:233/233 of 1sstA

query
sites
1sstA

V

V

W

W

H

Q

R

H

I

I

G

R

E

Q

E

E

A

A

R

K

L

E

A

L

V

A

A

E

D

N

E

E

Q

P

L

M

L

L

G

A

G

S

L

F

V

F

H

H

S

S

S

T

I

I

L

L

H

K

H

H

A

Q

T

N

F

L

E

G

A

G

A

A

L

L

A

S

Y

Y

R

L

I

L

T

A

L

N

K

K

L

L

S

A

S

N

P

P

E

I

M

M

S

P

A

A

Q

I

T

S

L

L

R

R

Q

E

L

I

V

I

D

E

D

E

A

A

F

Y

A

Q

S

S

D

N

A

P

T

S

L

I

G

I

V

D

A

C

A

A

R

A

A

C

D

D

L

I

V

Q

A

A

V

V

Y

R

E

H

R

R

D

D

P

P

A

A

C

V

H

E

R

L

L

W

M

S

Q

T

P

P

L

L

L

L

Y

Y

F

L

K

K

G

G

F

F

Q

H

A

A

V

I

Q

Q

A

S

Y

Y

R

R

V

I

G

T

H

H

W

Y

L

L

Y

W

S

N

Q

Q

G

N

R

R

H

K

D

S

L

L

A

A

Y

L

F

Y

V

L

Q

Q

M

N

R

Q

V

I

S

S

E

V

V

A

F

F

G

D

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

K

I

I

G

G

Q

H

G

G

I

I

M

M

I

F

D

D

H
|
H

A

A

H

T

S

G

I

I

V

V

I

V

G

G

E

E

T

T

A

S

V

V

V

I

G

E

D

N

N

D

V

V

S

S

M

I

L
|
L

H

Q

S

G

V

V

T

T

L

L

G

G

G
|
G

T
|
T

G

-

K

-

E

-

D

-

D

-

D

-

R

-

H

H

P

P

K

K

I

V

G

R

D

E

G

G

V

V

L

M

I

I

G

G

A
|
A

G

G

A

A

H

K

V

I

L
|
L

G

G

N

N

I

I

T

E

I

V

G

G

H

K

C

Y

S

A

R
x
K

I

I

A
x
G

A
|
A

G

N

S

S

V
|
V

V

V

L
|
L

S

N

D

P

V

V

P

P

P

E

C

Y

K

A

T
|
T

V

A

A
|
A

G

G

V
|
V

P
|
P

A

A

K

R

I

I

V
|
V

binding coenzyme a: H154 (= H163), L173 (= L182), G180 (= G189), T181 (= T190), A193 (= A209), L198 (= L214), K208 (≠ R224), G210 (≠ A226), A211 (= A227), V214 (= V230), L216 (= L232), T224 (= T240), A226 (= A242), V228 (= V244), P229 (= P245), V233 (= V249)

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
52% identity, 88% coverage: 14:249/269 of query aligns to 4:233/233 of 4n6bA

query
sites
4n6bA

V

V

W

W

H

G

R

Q

I

I

G

K

E

A

E

E

A

A

R

R

L

R

A

D

V

A

A

E

D

S

E

E

Q

P

L

A

L

L

G

A

G

S

L

Y

V

L

H

Y

S

S

S

T

I

I

L

L

H

S

H

H

A

S

T

S

F

L

E

E

A

R

A

S

L

L

A

S

Y

F

R

H

I

L

T

G

L

N

K

K

L

L

S

C

S

S

P

S

E

T

M

L

S

L

A

S

Q

T

T

L

L

L

R

Y

Q

D

L

L

V

F

D

L

D

N

A

A

F

F

A

S

S

S

D

D

A

P

T

S

L

L

G

R

V

S

A

A

A

A

R

V

A

A

D

D

L

L

V

R

A

A

V

A

Y

R

E

E

R

R

D

D

P

V

A

S

C

-

H

-

R

-

L

Y

M

S

Q

H

P

C

L

L

L

L

Y

N

F

Y

K

K

G

G

F

F

Q

L

A

A

V

C

Q

Q

A

A

Y

H

R

R

V

V

G

A

H

H

W

L

L

L

Y

W

S

R

Q

Q

G

S

R

R

H

R

D

P

L

L

A

A

Y

L

F

A

V

L

Q

H

M

S

R

R

V

I

S

A

E

N

V

V

F

F

G

A

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

R

I

I

G

G

Q

K

G

G

I

I

M

L

I

F

D

D

H
|
H

A

A

H

T

S

G

I

V

V

V

I

V

G

G

E

E

T

T

A

A

V

V

V

I

G

G

D

N

N

N

V

V

S

S

M

I

L
|
L

H

H

S

H

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

-

E

-

D

-

D

G

D

D

R

R

H

H

P

P

K

K

I

I

G

G

D

D

G

G

V

V

L

L

I

I

G

G

A

A

G

G

A

A

H

T

V

I

L

L

G

G

N

N

I

I

T

K

I

I

G

G

H

E

C

G

S

A

R
x
K

I

V

A
x
G

A
|
A

G

G

S

S

V

V

V

V

L
|
L

S

I

D

D

V

V

P

P

P

P

C

R

K

T

T

T

V

A

A
x
V

G

G

V

N

P

P

A

A

K

R

I

L

V

V

binding coenzyme a: H150 (= H163), L169 (= L182), G176 (= G189), K208 (≠ R224), G210 (≠ A226), A211 (= A227), L216 (= L232), V226 (≠ A242)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
38% identity, 64% coverage: 81:251/269 of query aligns to 98:270/280 of 7bw9A

query
sites
7bw9A

T

T

L

I

G

R

V

E

A

L

A

V

R

K

A

Q

D

D

L

I

V

I

A

A

V

A

Y

Y

E

T

R

G

D
|
D

P
|
P

A

A

C

A

H

S

R

S

L

L

M

A

Q

M

P

I

L

I

L

R

Y

S

F

Y

K

P

G

G

F

I

Q

H

A

V

V

M

Q

M

A

I

Y

Q

R

R

V

V

G

A

H

H

W

I

L

L

Y

Y

S

M

Q

N

G

G

R

D

H

I

D

E

L

Y

A

S

Y

R

F

E

V

L

Q

M

M

E

R

N

V

I

S

H

E

S

V

V

F

T

G

G

V

I

D

D

I

I

H

H

P

P

A

G

A

T

K

S

I

I

G

G

Q

N

G

H

I

F

M

F

I

I

D
|
D

H
|
H

A

G

H

V

S

G

I

V

V

V

I

I

G

G

E

E

T

T

A

A

V

V

V

I

G

G

D

N

N

W

V

C

S

R

M

V

L

Y

H

Q

S

S

V

V

T

T

L

L

G

G

G
x
A

T

M

G

S

K

F

E

K

D

G

D

N

D

K

R
|
R

H
|
H

P

P

K

T

I

I

G

G

D

D

G

F

V

V

L

V

I

I

G

G

A

A

G

G

A

A

H

K

V

V

L

L

G

G

N

N

I

I

T

T

I

I

G

G

H

S

C

N

S

V

R

K

I

I

A

G

A

A

G

N

S

C

V

W

V

I

L

T

S

Q

D

N

V

I

P

D

P

Q

C

D

K

Q

T

I

V

V

-

F

-

I

A

S

G

E

V

H

P

P

A

S

K

Q

I

I

V

T

G

K

E

E

binding cysteine: D114 (= D97), P115 (= P98), D179 (= D162), H180 (= H163), A206 (≠ G189), R214 (= R197), H215 (= H198)

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
40% identity, 58% coverage: 87:241/269 of query aligns to 106:268/270 of 3p47A

query
sites
3p47A

R

K

A

T

D

D

L

L

V

I

A

A

V

A

Y

Y

E

A

R

G

D

D

P

P

A

A

C

A

H

P

R

G

L

L

M

S

Q

L

P

I

L

I

L

R

Y

C

F

Y

K

P

G

G

F

F

Q

Q

A

A

V

V

Q

I

A

V

Y

Y

R

R

V

I

G

A

H

H

W

V

L

L

Y

Y

S

E

Q

C

G

G

R

E

H

R

D

Y

L

Y

A

C

Y

R

F

E

V

M

Q

M

M

E

R

S

V

V

S

H

E

S

V

Y

F

T

G

S

V

I

D

D

I

I

H

H

P

P

A

G

A

A

K

S

I

I

G

K

Q

G

G

H

I

F

M

F

I

I

D

D

H

H

A

G

H

V

S

G

I

V

V

V

I

I

G

G

E

E

T

T

A

A

V

I

V

I

G

G

D

E

N

W

V

C

S

R

M

I

L

Y

H

Q

S

S

V

V

T

T

L

L

-

G

-
x
A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

G

G

T

V

G

I

K

K

E

R

D

G

D

T

D

K

R
|
R

H
|
H

P

P

K

T

I

V

G

G

D

D

G

Y

V

V

L

T

I

I

G

G

A

T

G

G

A

A

H

K

V

V

L

L

G

G

N

N

I

I

T

I

I

V

G

G

H

S

C

H

S

V

R

R

I

I

A

G

A

A

G

N

S

C

V

W

V

I

L

D

S

R

D

D

V

V

P

D

P

S

C

N

K

Q

T

T

V

V

binding cysteine: A208 (vs. gap), H210 (vs. gap), R224 (= R197), H225 (= H198)

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
40% identity, 58% coverage: 87:241/269 of query aligns to 104:266/267 of 3q1xA

query
sites
3q1xA

R

K

A

T

D

D

L

L

V

I

A

A

V

A

Y

Y

E

A

R

G

D

D

P

P

A

A

C

A

H

P

R

G

L

L

M

S

Q

L

P

I

L

I

L

R

Y

C

F

Y

K

P

G

G

F

F

Q

Q

A

A

V

V

Q

I

A

V

Y

Y

R

R

V

I

G

A

H

H

W

V

L

L

Y

Y

S

E

Q

C

G

G

R

E

H

R

D

Y

L

Y

A

C

Y

R

F

E

V

M

Q

M

M

E

R

S

V

V

S

H

E

S

V

Y

F

T

G

S

V

I

D

D

I

I

H

H

P

P

A

G

A

A

K

S

I

I

G

K

Q

G

G

H

I

F

M

F

I

I

D

D

H

H

A

G

H

V

S

G

I

V

V

V

I

I

G

G

E

E

T

T

A

A

V

I

V

I

G

G

D

E

N

W

V

C

S

R

M

I

L

Y

H

Q

S

S

V

V

T

T

L

L

-

G

-

A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

G

G

T

V

G

I

K

K

E

R

D

G

D

T

D

K

R
|
R

H
|
H

P

P

K

T

I

V

G

G

D

D

G

Y

V

V

L

T

I

I

G

G

A

T

G

G

A

A

H

K

V

V

L

L

G

G

N

N

I

I

T

I

I

V

G

G

H

S

C

H

S

V

R

R

I

I

A

G

A

A

G

N

S

C

V

W

V

I

L

D

S

R

D

D

V

V

P

D

P

S

C

N

K

Q

T

T

V

V

binding serine: H208 (vs. gap), R222 (= R197), H223 (= H198)

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
33% identity, 43% coverage: 136:251/269 of query aligns to 62:183/201 of 1krvA

query
sites
1krvA

F

W

V

V

Q

E

M

P

R

P

V

V

S

Y

E

F

V
x
S

F

Y

G

G

V

S

D

N

I

I

H

H

P

-

A

-

A

-

K

-

I

I

G

G

Q

R

G

N

I

F

M
x
Y

I

A

D
x
N

H

F

A

N

H

L

S

T

I

I

V
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V

I

D

G
x
D

E

Y

T

T

A

V

V

T

V

I

G

G

D

D

N

N

V

V

S

L

M

I

L
x
A

H

P

S

N

V

V

T

T

L

L

G
x
S

G

V

T
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T

G

G

-

H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

D

N

D

G

D

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

K

T

I

I

G

G

D

N

G

N

V

V

L
x
W

I

I

G
|
G

A
x
S

G

H

A

V

H

V

V

I

L
x
N

G

P

N

G

I

V

T

T

I

I

G

G

H

D

C

N

S

S

R

V

I

I

A

G

A
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A

G

G

S

S

V

I

V

V

L

T

S

K

D

D

V

I

P

P

P

P

C

N

K

V

T

V

V

A

A

A

G

G

V

V

P
|
P

A

C

K
x
R

I

V

V

I

G
x
R

E

E

binding 4-nitrophenyl beta-D-galactopyranoside: S70 (≠ V144), Y82 (≠ M160), N84 (≠ D162), V90 (= V168), D92 (≠ G170), M126 (≠ R197)
binding coenzyme a: A104 (≠ L182), S110 (≠ G188), T112 (= T190), W138 (≠ L206), G140 (= G208), S141 (≠ A209), N146 (≠ L214), A159 (= A227), P177 (= P245), R179 (≠ K247), R182 (≠ G250)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
33% identity, 43% coverage: 136:251/269 of query aligns to 62:183/201 of 1kruA

query
sites
1kruA

F
x
W

V

V

Q

E

M

P

R

P

V

V

S

Y

E

F

V
x
S

F

Y

G

G

V

S

D

N

I

I

H

H

P

-

A

-

A

-

K

-

I

I

G

G

Q

R

G

N

I

F

M
x
Y

I

A

D
x
N

H

F

A

N

H

L

S

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I

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

V

T

V

I

G

G

D

D

N

N

V

V

S
x
L

M

I

L

A

H

P

S

N

V

V

T

T

L

L

G

S

G

V

T

T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

D

N

D

G

D

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

K

T

I

I

G

G

D

N

G

N

V

V

L
x
W

I

I

G
|
G

A
x
S

G

H

A

V

H

V

V

I

L

N

G

P

N

G

I

V

T

T

I

I

G

G

H

D

C

N

S

S

R

V

I

I

A
x
G

A
|
A

G

G

S

S

V

I

V

V

L

T

S

K

D

D

V

I

P

P

P

P

C

N

K

V

T

V

V

A

A

A

G

G

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P
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P

A

C

K

R

I

V

V

I

G
x
R

E

E

binding coenzyme a: H114 (vs. gap), W138 (≠ L206), G140 (= G208), S141 (≠ A209), G158 (≠ A226), A159 (= A227), P177 (= P245), R182 (≠ G250)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: W62 (≠ F136), S70 (≠ V144), Y82 (≠ M160), Y82 (≠ M160), N84 (≠ D162), V90 (= V168), D92 (≠ G170), L102 (≠ S180), H114 (vs. gap), M126 (≠ R197)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
33% identity, 43% coverage: 136:251/269 of query aligns to 62:183/200 of 1krrA

query
sites
1krrA

F

W

V

V

Q

E

M

P

R

P

V

V

S

Y

E

F

V

S

F

Y

G

G

V

S

D

N

I

I

H

H

P

-

A

-

A

-

K

-

I

I

G

G

Q

R

G

N

I

F

M

Y

I

A

D
x
N

H

F

A

N

H

L

S

T

I

I

V

V

I

D

G

D

E

Y

T

T

A

V

V

T

V

I

G

G

D

D

N

N

V

V

S

L

M
x
I

L
x
A

H
x
P

S

N

V

V

T

T

L

L

G

S

G

V

T
|
T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

D

N

D

G

D

E

R

M

H

Y

P

S

-

F

-

P

-

I

K

T

I

I

G

G

D

N

G

N

V

V

L

W

I

I

G
|
G

A
x
S

G

H

A

V

H

V

V

I

L
x
N

G

P

N

G

I

V

T

T

I

I

G

G

H

D

C

N

S

S

R

V

I

I

A
x
G

A
|
A

G

G

S

S

V

I

V

V

L

T

S

K

D

D

V

I

P

P

P

P

C

N

K

V

T

V

V

A

A
|
A

G

G

V

V

P
|
P

A

C

K

R

I

V

V

I

G
x
R

E

E

binding acetyl coenzyme *a: N84 (≠ D162), I103 (≠ M181), A104 (≠ L182), P105 (≠ H183), T112 (= T190), H114 (vs. gap), G140 (= G208), S141 (≠ A209), N146 (≠ L214), G158 (≠ A226), A159 (= A227), A174 (= A242), P177 (= P245), R182 (≠ G250)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
33% identity, 43% coverage: 136:251/269 of query aligns to 63:184/203 of P07464

query
sites
P07464

F

W

V

V

Q

E

M

P

R

P

V

V

S

Y

E

F

V
x
S

F

Y

G

G

V

S

D

N

I

I

H

H

P

-

A

-

A

-

K

-

I

I

G

G

Q

R

G

N

I

F

M

Y

I

A

D
x
N

H

F

A

N

H

L

S

T

I

I

V

V

I

D

G
x
D

E

Y

T

T

A

V

V

T

V

I

G

G

D

D

N

N

V

V

S

L

M

I

L

A

H

P

S

N

V

V

T

T

L

L

G

S

G

V

T

T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

D

N

D

G

D

E

R

M

H

Y

P

S

-

F

-

P

-

I

K

T

I

I

G

G

D

N

G

N

V

V

L

W

I

I

G

G

A
x
S

G

H

A

V

H

V

V

I

L

N

G

P

N

G

I

V

T

T

I

I

G

G

H

D

C

N

S

S

R

V

I

I

A

G

A
|
A

G

G

S

S

V

I

V

V

L
x
T

S
x
K

D

D

V

I

P

P

P

P

C

N

K

V

T

V

V

A

A

A

G

G

V

V

P

P

A

C

K
x
R

I

V

V

I

G
x
R

E

E

S71 (≠ V144) binding
N85 (≠ D162) binding in other chain; binding in other chain
D93 (≠ G170) binding
H115 (vs. gap) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A209) binding in other chain
A160 (= A227) binding in other chain
TK 165:166 (≠ LS 232:233) binding
R180 (≠ K247) binding
R183 (≠ G250) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding

Query Sequence

>3608769 FitnessBrowser__Dino:3608769
MGKASKSLKAVDPVWHRIGEEARLAVADEQLLGGLVHSSILHHATFEAALAYRITLKLSS
PEMSAQTLRQLVDDAFASDATLGVAARADLVAVYERDPACHRLMQPLLYFKGFQAVQAYR
VGHWLYSQGRHDLAYFVQMRVSEVFGVDIHPAAKIGQGIMIDHAHSIVIGETAVVGDNVS
MLHSVTLGGTGKEDDDRHPKIGDGVLIGAGAHVLGNITIGHCSRIAAGSVVLSDVPPCKT
VAGVPAKIVGEAGCAQPSVTMDQLLSESI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory