SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3608830 FitnessBrowser__Dino:3608830 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3i6vA Crystal structure of a periplasmic his/glu/gln/arg/opine family- binding protein from silicibacter pomeroyi in complex with lysine
75% identity, 87% coverage: 26:236/242 of query aligns to 1:211/218 of 3i6vA

query
sites
3i6vA

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binding lysine: E7 (= E32), Y10 (= Y35), W48 (= W73), G66 (= G91), M67 (= M92), S68 (= S93), R73 (= R98), Q107 (= Q133), T110 (= T136), I111 (= I137), Q112 (= Q138)

5itoA Structure of the periplasmic binding protein m117n-noct from a. Tumefaciens in complex with octopine (see paper)
31% identity, 88% coverage: 23:236/242 of query aligns to 1:249/255 of 5itoA

query
sites
5itoA

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binding octopine: E10 (= E32), Y13 (= Y35), W51 (= W73), A68 (= A90), A69 (≠ G91), G71 (≠ S93), R76 (= R98), Q139 (= Q133), T142 (= T136), S143 (≠ I137), H144 (≠ Q138), S181 (≠ D173)

5ovzA High resolution structure of the pbp noct in complex with nopaline (see paper)
31% identity, 88% coverage: 23:236/242 of query aligns to 1:249/259 of 5ovzA

query
sites
5ovzA

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binding N-[(1S)-4-carbamimidamido-1-carboxybutyl]-D-glutamic acid: E10 (= E32), Y13 (= Y35), W51 (= W73), A68 (= A90), A69 (≠ G91), G71 (≠ S93), R76 (= R98), M91 (≠ S114), Q139 (= Q133), T142 (= T136), S143 (≠ I137), H144 (≠ Q138), S181 (≠ D173), V213 (≠ I200)

P02911 Lysine/arginine/ornithine-binding periplasmic protein; LAO-binding protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
31% identity, 97% coverage: 2:236/242 of query aligns to 3:253/260 of P02911

query
sites
P02911

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D33 (≠ E32) binding ; binding ; binding ; mutation to A: Decreases arginine and histidine binding affinity.
Y36 (= Y35) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
D52 (≠ E51) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
C60 (= C59) modified: Disulfide link with 67
C67 (= C66) modified: Disulfide link with 60
F74 (≠ W73) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
S91 (≠ A90) binding ; binding ; mutation to A: Has no effect on the arginine and histidine binding affinity.
S92 (≠ G91) binding ; binding ; binding ; mutation to A: Decreases arginine and histidine binding affinity.
S94 (= S93) binding ; binding ; binding ; mutation to A: Decreases arginine and histidine binding affinity.
R99 (= R98) binding ; binding ; binding ; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
L139 (≠ Q133) mutation to K: Changes ligand specificity. Can bind glutamine. Still able to bind basic amino acids, however, with 1000-fold less affinity for arginine.; mutation to Q: Does not affect ligand preference.
T143 (≠ I137) binding ; binding ; binding
D183 (= D173) binding ; binding ; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.

5otcA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with noroctopinic acid. (see paper)
31% identity, 87% coverage: 26:236/242 of query aligns to 3:248/256 of 5otcA

query
sites
5otcA

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P

D

R

V

M

-

T

-

G

L

L

L

F

G

G

G

K

G

G

I

V

G

G

M

V

G

G

L

I

R

R

E

K

S

E

D

D

P

A

E

D

L

L

R

K

A

A

K

L

F

F

D

D

A

K

A

A

I

I

Q

D

S

A

M

A

K

I

D

A

D

D

G

G

S

T

L

V

N

Q

A

K

L

L

I

S

A

Q

K

Q

W

W

L

F

binding (2~{S})-5-azanyl-2-(2-hydroxy-2-oxoethylamino)pentanoic acid: E9 (= E32), Y12 (= Y35), W50 (= W73), A68 (≠ G91), G70 (≠ S93), R75 (= R98), Q138 (= Q133), T141 (= T136), S142 (≠ I137), H143 (≠ Q138), S180 (≠ D173)

5otaA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with octopinic acid (see paper)
31% identity, 87% coverage: 26:236/242 of query aligns to 3:248/254 of 5otaA

query
sites
5otaA

T

S

V

I

R

T

M

I

G

A

T

T

E
|
E

G

G

A

S

Y
|
Y

P

A

P

P

Y

Y

N

N

F

F

L

K

N

D

D

A

A

G

G

G

E

K

V

L

D

I

G

G

F

F

E

D

R

I

E

D

L

L

G

G

D

N

E

D

L

L

C

C

A

K

R

R

A

M

E

N

L

I

T

E

C

C

E

K

W

F

V

V

T

E

N

Q

D

A

W
|
W

D

D

S

G

I

I

P

P

N

S

L

L

V

T

S

A

G

G

N

R

Y

Y

D

D

T

A

I

I

I

M

A

A

G
x
A

M

M

S
x
G

I

I

T

Q

D

P

E

A

R
|
R

D

E

E

K

V

V

I

I

D

A

F

F

T

S

Q

R

N

P

Y

Y

T

L

Q

L

P

-

D

T

P

P

S
x
M

A

T

F

F

M

L

G

T

L

T

S

A

-

D

-

S

-

P

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

E

E

D

N

V

L

D

P

L

L

-

D

-

N

-

I

-

T

-

P

-

E

-

Q

-

K

-

A

-

E

-

L

-

D

-

K

-

F

-

T

-

K

-

I

-

F

E

E

G

G

G

V

V

K

I

F

A

G

A

V

Q
|
Q

T

A

G

G

T
|
T

I
x
S

Q
x
H

A

E

S

A

H

F

I

M

A

K

S

Q

T

M

-

M

-

P

G

S

A

V

T

Q

L

I

I

S

E

T

F

Y

A

D

S

T

P

I

D

D

E

N

T

V

V

V

A

M

A

D

V

L

R

K

N

A

G

G

E

R

A

I

D

D

A

A

V

S

L

L

A

A

D
x
S

K

V

A

S

F

F

L

L

E

K

P

P

F

L

V

T

-

D

-

K

T

P

E

D

N

N

A

K

D

D

L

L

V

K

F

M

V

F

G

G

E

P

D

R

V

M

-

T

-

G

L

L

L

F

G

G

G

K

G

G

I

V

G

G

M

V

G

G

L

I

R

R

E

K

S

E

D

D

P

A

E

D

L

L

R

K

A

A

K

L

F

F

D

D

A

K

A

A

I

I

Q

D

S

A

M

A

K

I

D

A

D

D

G

G

S

T

L

V

N

Q

A

K

L

L

I

S

A

Q

K

Q

W

W

L

F

binding (2~{S})-5-azanyl-2-[[(2~{R})-1-oxidanyl-1-oxidanylidene-propan-2-yl]amino]pentanoic acid: E9 (= E32), Y12 (= Y35), W50 (= W73), A68 (≠ G91), G70 (≠ S93), R75 (= R98), M90 (≠ S114), Q138 (= Q133), T141 (= T136), S142 (≠ I137), H143 (≠ Q138), S180 (≠ D173)

5ot9A Structure of the periplasmic binding protein (pbp) noct from a.Tumefaciens c58 in complex with histopine. (see paper)
31% identity, 87% coverage: 26:236/242 of query aligns to 3:248/254 of 5ot9A

query
sites
5ot9A

T

S

V

I

R

T

M

I

G

A

T

T

E
|
E

G

G

A

S

Y
|
Y

P

A

P

P

Y

Y

N

N

F

F

L

K

N

D

D

A

A

G

G

G

E

K

V

L

D

I

G

G

F

F

E

D

R

I

E

D

L

L

G

G

D

N

E

D

L

L

C

C

A

K

R

R

A

M

E

N

L

I

T

E

C

C

E

K

W

F

V

V

T

E

N

Q

D

A

W
|
W

D

D

S

G

I

I

P

P

N

S

L

L

V

T

S

A

G

G

N

R

Y

Y

D

D

T

A

I

I

I

M

A
|
A

G
x
A

M

M

S

G

I

I

T

Q

D

P

E

A

R
|
R

D

E

E

K

V

V

I

I

D

A

F

F

T

S

Q

R

N

P

Y

Y

T

L

Q

L

P

-

D

T

P

P

S
x
M

A

T

F

F

M

L

G

T

L

T

S

A

-

D

-

S

-

P

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

E

E

D

N

V

L

D

P

L

L

-

D

-

N

-

I

-

T

-

P

-

E

-

Q

-

K

-

A

-

E

-

L

-

D

-

K

-

F

-

T

-

K

-

I

-

F

E

E

G

G

G

V

V

K

I

F

A

G

A

V

Q
|
Q

T

A

G

G

T
|
T

I
x
S

Q
x
H

A

E

S

A

H

F

I

M

A

K

S

Q

T

M

-

M

-

P

G

S

A

V

T

Q

L

I

I

S

E

T

F

Y

A

D

S

T

P

I

D

D

E

N

T

V

V

V

A

M

A

D

V

L

R

K

N

A

G

G

E

R

A

I

D

D

A

A

V

S

L

L

A

A

D
x
S

K

V

A

S

F

F

L

L

E

K

P

P

F

L

V

T

-

D

-

K

T

P

E

D

N

N

A

K

D

D

L

L

V

K

F

M

V

F

G

G

E

P

D

R

V

M

-

T

-

G

L

L

L

F

G

G

G

K

G

G

I

V

G

G

M

V

G

G

L

I

R

R

E

K

S

E

D

D

P

A

E

D

L

L

R

K

A

A

K

L

F

F

D

D

A

K

A

A

I

I

Q

D

S

A

M

A

K

I

D

A

D

D

G

G

S

T

L

V

N

Q

A

K

L

L

I

S

A

Q

K

Q

W

W

L

F

binding Histopine: E9 (= E32), Y12 (= Y35), W50 (= W73), A67 (= A90), A68 (≠ G91), R75 (= R98), M90 (≠ S114), Q138 (= Q133), T141 (= T136), S142 (≠ I137), H143 (≠ Q138), S180 (≠ D173)

4powA Structure of the pbp noct in complex with pyronopaline (see paper)
31% identity, 87% coverage: 26:236/242 of query aligns to 3:248/254 of 4powA

query
sites
4powA

T

S

V

I

R

T

M

I

G

A

T

T

E
|
E

G

G

A

S

Y
|
Y

P

A

P

P

Y

Y

N

N

F

F

L

K

N

D

D

A

A

G

G

G

E

K

V

L

D

I

G

G

F

F

E

D

R

I

E

D

L

L

G

G

D

N

E

D

L

L

C

C

A

K

R

R

A

M

E

N

L

I

T

E

C

C

E

K

W

F

V

V

T

E

N

Q

D

A

W
|
W

D

D

S

G

I

I

P

P

N

S

L

L

V

T

S

A

G

G

N

R

Y

Y

D

D

T

A

I

I

I

M

A
|
A

G
x
A

M

M

S
x
G

I

I

T

Q

D

P

E

A

R
|
R

D

E

E

K

V

V

I

I

D

A

F

F

T

S

Q

R

N

P

Y

Y

T

L

Q

L

P

-

D

T

P

P

S
x
M

A

T

F

F

M

L

G

T

L

T

S

A

-

D

-

S

-

P

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

E

E

D

N

V

L

D

P

L

L

-

D

-

N

-

I

-

T

-

P

-

E

-

Q

-

K

-

A

-

E

-

L

-

D

-

K

-

F

-

T

-

K

-

I

-

F

E

E

G

G

G

V

V

K

I

F

A

G

A

V

Q
|
Q

T

A

G

G

T
|
T

I
x
S

Q
x
H

A

E

S

A

H

F

I

M

A

K

S

Q

T

M

-

M

-

P

G

S

A

V

T

Q

L

I

I

S

E

T

F

Y

A

D

S

T

P

I

D

D

E

N

T

V

V

V

A

M

A

D

V

L

R

K

N

A

G

G

E

R

A

I

D

D

A

A

V

S

L

L

A

A

D
x
S

K

V

A

S

F

F

L

L

E

K

P

P

F

L

V

T

-

D

-

K

T

P

E

D

N

N

A

K

D

D

L

L

V

K

F

M

V

F

G

G

E

P

D

R

V

M

-

T

-

G

L

L

L

F

G

G

G

K

G

G

I

V

G

G

M

V

G

G

L

I

R

R

E

K

S

E

D

D

P

A

E

D

L

L

R

K

A

A

K

L

F

F

D

D

A

K

A

A

I

I

Q

D

S

A

M

A

K

I

D

A

D

D

G

G

S

T

L

V

N

Q

A

K

L

L

I

S

A

Q

K

Q

W

W

L

F

binding 1-[(1S)-4-carbamimidamido-1-carboxybutyl]-5-oxo-D-proline: E9 (= E32), Y12 (= Y35), W50 (= W73), A67 (= A90), A68 (≠ G91), G70 (≠ S93), R75 (= R98), M90 (≠ S114), Q138 (= Q133), T141 (= T136), S142 (≠ I137), H143 (≠ Q138), S180 (≠ D173)

4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
31% identity, 87% coverage: 26:236/242 of query aligns to 5:223/225 of 4zv2A

query
sites
4zv2A

T

T

V

L

R

R

M

V

G

G

T

T

E
|
E

G

A

A

T

Y
x
F

P

P

Y

F

N

G

F

F

L

K

N

D

D

E

A

N

G

G

E

K

V

L

D

V

G

G

F

F

E

D

R

I

E

D

L

L

G

A

D

K

E

A

L

I

C

A

A

K

R

K

A

L

E

G

L

V

T

K

C

V

E

E

W

F

V

K

T

P

N

M

D

D

W
x
F

D

D

S

G

I

I

P

P

N

A

L

L

V

Q

S

S

G

G

N

K

Y

I

D

D

T

V

I

V

I

I

A

A

G
|
G

M
|
M

S
x
T

I

I

T

T

D

E

E

E

R
|
R

D

K

E

K

V

Q

I

V

D

D

F

F

T

S

Q

D

N

P

Y

Y

T

F

Q

E

P

A

D

G

P

Q

S

A

A

I

F

V

M

V

G

K

L

K

S

G

E

N

D

D

V

S

-

I

-

K

-

S

-

L

-

E

D

D

L

L

E

K

G

G

G

K

V

K

I

V

A

G

A

V

Q

Q

T

L

G

G

T
x
S

I
x
T

Q

S

A

E

S

Q

H

H

I

V

A

K

S

K

T

V

-

A

G

G

A

V

T

K

L

V

I

K

E

K

F

F

A

D

S

N

P

F

D

S

E

E

T

A

V

F

A

Q

A

E

V

L

R

K

N

S

G

G

E

R

A

V

D

D

A

A

V

V

L

V

A

T

D
|
D

K

N

A

A

F

V

L

A

E

L

P

A

F

Y

V

V

T

K

E

Q

-

N

-

P

N

N

A

A

D

G

L

V

V

K

F

I

V

V

G

G

E

E

D

-

V

T

L

F

L

S

G

G

G

E

G

P

I

Y

G

G

M

I

G

A

L

V

R

R

E

K

S

G

D

N

P

S

E

E

L

L

R

L

A

E

K

K

F

I

D

N

A

K

A

A

I

L

Q

E

S

E

M

M

K

K

D

K

D

D

G

G

S

T

L

Y

N

D

A

K

L

I

I

Y

A

E

K

K

W

W

L

F

binding glutamine: E11 (= E32), F14 (≠ Y35), F52 (≠ W73), G70 (= G91), M71 (= M92), T72 (≠ S93), R77 (= R98), S120 (≠ T136), T121 (≠ I137), D159 (= D173)

5owfA Structure of a lao-binding protein mutant with glutamine (see paper)
31% identity, 87% coverage: 26:236/242 of query aligns to 2:228/235 of 5owfA

query
sites
5owfA

T

T

V

V

R

R

M

I

G

G

T

T

E

D

G

T

A

T

Y
|
Y

P

A

P

P

Y

F

N

S

F

S

L

K

N

D

D

A

A

K

G

G

E

E

V

F

D

I

G

G

F

F

E

D

R

I

E

D

L

L

G

G

D

N

E

E

L

M

C

C

A

K

R

R

A

M

E

Q

L

V

T

K

C

C

E

T

W

W

V

V

T

A

N

S

D

D

W
x
F

D

D

S

A

I

L

I

I

P

P

N

S

L

L

V

K

S

A

G

K

N

K

Y

I

D

D

T

A

I

I

A
x
S

G
x
S

M

L

S
|
S

I

I

T

T

D

D

E

K

R
|
R

D

Q

E

Q

V

E

I

I

D

A

F

F

T

S

Q

D

N

K

Y

L

T

Y

Q

A

P

A

D

D

P

S

S

R

A

L

F

I

M

A

G

A

L

K

S

G

E

S

D

P

V

I

D

Q

-

P

-

T

-

L

-

E

-

S

L

L

E

K

G

G

G

K

V

H

I

V

A

G

A

V

Q
x
K

T

Q

G

G

T
x
S

I
x
T

Q

Q

A

E

S

A

H

Y

I

A

-

N

-

D

-

N

-

W

A

R

S

T

T

K

G

G

A

V

T

D

L

V

I

V

E

A

F

Y

A

A

S

N

P

Q

D

D

E

L

T

I

V

Y

A

S

A

D

V

L

R

T

N

A

G

G

E

R

A

L

D

D

A

A

V

A

L

L

A

Q

D
|
D

K

E

-

V

A

A

F

A

L

S

E

E

P

G

F

F

V

L

T

K

E

Q

N

P

A

A

-

G

-

K

D

E

L

Y

V

A

F

F

V

A

G

G

E

P

D

S

V

V

-

K

-

D

-

K

L

Y

L

F

G

G

G

D

G

G

I

T

G

G

M

V

G

G

L

L

R

R

E

K

S

D

D

D

P

T

E

E

L

L

R

K

A

A

K

A

F

F

D

D

A

K

A

A

I

L

Q

T

S

E

M

L

K

R

D

Q

D

D

G

G

S

T

L

Y

N

D

A

K

L

M

I

A

A

K

K

K

W

Y

L

F

binding glutamine: Y11 (= Y35), F49 (≠ W73), S66 (≠ A90), S67 (≠ G91), S69 (= S93), R74 (= R98), K114 (≠ Q133), S117 (≠ T136), T118 (≠ I137), D158 (= D173)

4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
31% identity, 87% coverage: 26:236/242 of query aligns to 5:225/226 of 4zv1A

query
sites
4zv1A

T

T

V

L

R

R

M

V

G

G

T

T

E
|
E

G

A

A

T

Y
x
F

P

P

Y

F

N

G

F

F

L

K

N

D

D

E

A

N

G

G

E

K

V

L

D

V

G

G

F

F

E

D

R

I

E

D

L

L

G

A

D

K

E

A

L

I

C

A

A

K

R

K

A

L

E

G

L

V

T

K

C

V

E

E

W

F

V

K

T

P

N

M

D

D

W
x
F

D

D

S

G

I

I

P

P

N

A

L

L

V

Q

S

S

G

G

N

K

Y

I

D

D

T

V

I

V

I

I

A
|
A

G
|
G

M
|
M

S
x
T

I

I

T

T

D

E

E

E

R
|
R

D

K

E

K

V

Q

I

V

D

D

F

F

T

S

Q

D

N

P

Y

Y

T

F

Q

E

P

A

D

G

P

Q

S

A

A

I

F

V

M

V

G

K

L

K

S

G

E

N

D

D

V

S

-

I

-

K

-

S

-

L

-

E

D

D

L

L

E

K

G

G

G

K

V

K

I

V

A

G

A

V

Q
|
Q

T

L

G

G

T
x
S

I
x
T

Q

S

A

E

S

Q

H

H

I

V

A

K

S

K

T

V

-

A

-

K

-

D

-

A

G

G

A

V

T

K

L

V

I

K

E

K

F

F

A

D

S

N

P

F

D

S

E

E

T

A

V

F

A

Q

A

E

V

L

R

K

N

S

G

G

E

R

A

V

D

D

A

A

V

V

L

V

A

T

D
|
D

K

N

A

A

F

V

L

A

E

L

P

A

F

Y

V

V

T

K

E

Q

-

N

-

P

N

N

A

A

D

G

L

V

V

K

F

I

V

V

G

G

E

E

D

-

V

T

L

F

L

S

G

G

G

E

G

P

I

Y

G

G

M

I

G

A

L

V

R

R

E

K

S

G

D

N

P

S

E

E

L

L

R

L

A

E

K

K

F

I

D

N

A

K

A

A

I

L

Q

E

S

E

M

M

K

K

D

K

D

D

G

G

S

T

L

Y

N

D

A

K

L

I

I

Y

A

E

K

K

W

W

L

F

binding arginine: E11 (= E32), F14 (≠ Y35), F52 (≠ W73), A69 (= A90), G70 (= G91), M71 (= M92), T72 (≠ S93), R77 (= R98), Q117 (= Q133), S120 (≠ T136), T121 (≠ I137), D161 (= D173)

1lstA Three-dimensional structures of the periplasmic lysine-, arginine-, ornithine-binding protein with and without a ligand (see paper)
31% identity, 87% coverage: 26:236/242 of query aligns to 5:231/238 of 1lstA

query
sites
1lstA

T

T

V

V

R

R

M

I

G

G

T

T

E

D

G

T

A

T

Y
|
Y

P

A

P

P

Y

F

N

S

F

S

L

K

N

D

D

A

A

K

G

G

E

E

V

F

D

I

G

G

F

F

E

D

R

I

E

D

L

L

G

G

D

N

E

E

L

M

C

C

A

K

R

R

A

M

E

Q

L

V

T

K

C

C

E

T

W

W

V

V

T

A

N

S

D

D

W
x
F

D

D

S

A

I

L

I

I

P

P

N

S

L

L

V

K

S

A

G

K

N

K

Y

I

D

D

T

A

I

I

A

S

G
x
S

M

L

S
|
S

I

I

T

T

D

D

E

K

R
|
R

D

Q

E

Q

V

E

I

I

D

A

F

F

T

S

Q

D

N

K

Y

L

T

Y

Q

A

P

A

D

D

P

S

S

R

A

L

F

I

M

A

G

A

L

K

S

G

E

S

D

P

V

I

D

Q

-

P

-

T

-

L

-

E

-

S

L

L

E

K

G

G

G

K

V

H

I

V

A

G

A

V

Q
x
L

T

Q

G

G

T
x
S

I
x
T

Q
|
Q

A

E

S

A

H

Y

I

A

-

N

-

D

-

N

-

W

A

R

S

T

T

K

G

G

A

V

T

D

L

V

I

V

E

A

F

Y

A

A

S

N

P

Q

D

D

E

L

T

I

V

Y

A

S

A

D

V

L

R

T

N

A

G

G

E

R

A

L

D

D

A

A

V

A

L

L

A

Q

D
|
D

K

E

-

V

A

A

F

A

L

S

E

E

P

G

F

F

V

L

T

K

E

Q

N

P

A

A

-

G

-

K

D

E

L

Y

V

A

F

F

V

A

G

G

E

P

D

S

V

V

-

K

-

D

-

K

L

Y

L

F

G

G

G

D

G

G

I

T

G

G

M

V

G

G

L

L

R

R

E

K

S

D

D

D

P

T

E

E

L

L

R

K

A

A

K

A

F

F

D

D

A

K

A

A

I

L

Q

T

S

E

M

L

K

R

D

Q

D

D

G

G

S

T

L

Y

N

D

A

K

L

M

I

A

A

K

K

K

W

Y

L

F

binding lysine: Y14 (= Y35), F52 (≠ W73), S70 (≠ G91), S72 (= S93), R77 (= R98), L117 (≠ Q133), S120 (≠ T136), T121 (≠ I137), Q122 (= Q138), D161 (= D173)

1lahE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
31% identity, 87% coverage: 26:236/242 of query aligns to 5:231/238 of 1lahE

query
sites
1lahE

T

T

V

V

R

R

M

I

G

G

T

T

E

D

G

T

A

T

Y
|
Y

P

A

P

P

Y

F

N

S

F

S

L

K

N

D

D

A

A

K

G

G

E

E

V

F

D

I

G

G

F

F

E

D

R

I

E

D

L

L

G

G

D

N

E

E

L

M

C

C

A

K

R

R

A

M

E

Q

L

V

T

K

C

C

E

T

W

W

V

V

T

A

N

S

D

D

W
x
F

D

D

S

A

I

L

I

I

P

P

N

S

L

L

V

K

S

A

G

K

N

K

Y

I

D

D

T

A

I

I

A

S

G
x
S

M

L

S
|
S

I

I

T

T

D

D

E

K

R
|
R

D

Q

E

Q

V

E

I

I

D

A

F

F

T

S

Q

D

N

K

Y

L

T

Y

Q

A

P

A

D

D

P

S

S

R

A

L

F

I

M

A

G

A

L

K

S

G

E

S

D

P

V

I

D

Q

-

P

-

T

-

L

-

E

-

S

L

L

E

K

G

G

G

K

V

H

I

V

A

G

A

V

Q

L

T

Q

G

G

T
x
S

I

T

Q
|
Q

A

E

S

A

H

Y

I

A

-

N

-

D

-

N

-

W

A

R

S

T

T

K

G

G

A

V

T

D

L

V

I

V

E

A

F

Y

A

A

S

N

P

Q

D

D

E

L

T

I

V

Y

A

S

A

D

V

L

R

T

N

A

G

G

E

R

A

L

D

D

A

A

V

A

L

L

A

Q

D
|
D

K

E

-

V

A

A

F

A

L

S

E

E

P

G

F

F

V

L

T

K

E

Q

N

P

A

A

-

G

-

K

D

E

L

Y

V

A

F

F

V

A

G

G

E

P

D

S

V

V

-

K

-

D

-

K

L

Y

L

F

G

G

G

D

G

G

I

T

G

G

M

V

G

G

L

L

R

R

E

K

S

D

D

D

P

T

E

E

L

L

R

K

A

A

K

A

F

F

D

D

A

K

A

A

I

L

Q

T

S

E

M

L

K

R

D

Q

D

D

G

G

S

T

L

Y

N

D

A

K

L

M

I

A

A

K

K

K

W

Y

L

F

binding L-ornithine: Y14 (= Y35), F52 (≠ W73), S70 (≠ G91), S72 (= S93), R77 (= R98), S120 (≠ T136), Q122 (= Q138), D161 (= D173)

1lagE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
31% identity, 87% coverage: 26:236/242 of query aligns to 5:231/238 of 1lagE

query
sites
1lagE

T

T

V

V

R

R

M

I

G

G

T

T

E

D

G

T

A

T

Y
|
Y

P

A

P

P

Y

F

N

S

F

S

L

K

N

D

D

A

A

K

G

G

E

E

V

F

D

I

G

G

F

F

E

D

R

I

E

D

L

L

G

G

D

N

E

E

L

M

C

C

A

K

R

R

A

M

E

Q

L

V

T

K

C

C

E

T

W

W

V

V

T

A

N

S

D

D

W
x
F

D

D

S

A

I

L

I

I

P

P

N

S

L

L

V

K

S

A

G

K

N

K

Y

I

D

D

T

A

I

I

A
x
S

G
x
S

M
x
L

S
|
S

I

I

T

T

D

D

E

K

R
|
R

D

Q

E

Q

V

E

I

I

D

A

F

F

T

S

Q

D

N

K

Y

L

T

Y

Q

A

P

A

D

D

P

S

S

R

A

L

F

I

M

A

G

A

L

K

S

G

E

S

D

P

V

I

D

Q

-

P

-

T

-

L

-

E

-

S

L

L

E

K

G

G

G

K

V

H

I

V

A

G

A

V

Q
x
L

T

Q

G

G

T
x
S

I

T

Q
|
Q

A

E

S

A

H

Y

I

A

-

N

-

D

-

N

-

W

A

R

S

T

T

K

G

G

A

V

T

D

L

V

I

V

E

A

F

Y

A

A

S

N

P

Q

D

D

E

L

T

I

V

Y

A

S

A

D

V

L

R

T

N

A

G

G

E

R

A

L

D

D

A

A

V

A

L

L

A

Q

D
|
D

K

E

-

V

A

A

F

A

L

S

E

E

P

G

F

F

V

L

T

K

E

Q

N

P

A

A

-

G

-

K

D

E

L

Y

V

A

F

F

V

A

G

G

E

P

D

S

V

V

-

K

-

D

-

K

L

Y

L

F

G

G

G

D

G

G

I

T

G

G

M

V

G

G

L

L

R

R

E

K

S

D

D

D

P

T

E

E

L

L

R

K

A

A

K

A

F

F

D

D

A

K

A

A

I

L

Q

T

S

E

M

L

K

R

D

Q

D

D

G

G

S

T

L

Y

N

D

A

K

L

M

I

A

A

K

K

K

W

Y

L

F

binding histidine: Y14 (= Y35), F52 (≠ W73), S69 (≠ A90), S70 (≠ G91), L71 (≠ M92), S72 (= S93), R77 (= R98), L117 (≠ Q133), S120 (≠ T136), Q122 (= Q138), D161 (= D173)

1lafE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
31% identity, 87% coverage: 26:236/242 of query aligns to 5:231/238 of 1lafE

query
sites
1lafE

T

T

V

V

R

R

M

I

G

G

T

T

E
x
D

G

T

A

T

Y
|
Y

P

A

P

P

Y

F

N

S

F

S

L

K

N

D

D

A

A

K

G

G

E

E

V

F

D

I

G

G

F

F

E

D

R

I

E

D

L

L

G

G

D

N

E

E

L

M

C

C

A

K

R

R

A

M

E

Q

L

V

T

K

C

C

E

T

W

W

V

V

T

A

N

S

D

D

W
x
F

D

D

S

A

I

L

I

I

P

P

N

S

L

L

V

K

S

A

G

K

N

K

Y

I

D

D

T

A

I

I

A
x
S

G
x
S

M

L

S
|
S

I

I

T

T

D

D

E

K

R
|
R

D

Q

E

Q

V

E

I

I

D

A

F

F

T

S

Q

D

N

K

Y

L

T

Y

Q

A

P

A

D

D

P

S

S

R

A

L

F

I

M

A

G

A

L

K

S

G

E

S

D

P

V

I

D

Q

-

P

-

T

-

L

-

E

-

S

L

L

E

K

G

G

G

K

V

H

I

V

A

G

A

V

Q
x
L

T

Q

G

G

T
x
S

I
x
T

Q
|
Q

A

E

S

A

H

Y

I

A

-

N

-

D

-

N

-

W

A

R

S

T

T

K

G

G

A

V

T

D

L

V

I

V

E

A

F

Y

A

A

S

N

P

Q

D

D

E

L

T

I

V

Y

A

S

A

D

V

L

R

T

N

A

G

G

E

R

A

L

D

D

A

A

V

A

L

L

A

Q

D
|
D

K

E

-

V

A

A

F

A

L

S

E

E

P

G

F

F

V

L

T

K

E

Q

N

P

A

A

-

G

-

K

D

E

L

Y

V

A

F

F

V

A

G

G

E

P

D

S

V

V

-

K

-

D

-

K

L

Y

L

F

G

G

G

D

G

G

I

T

G

G

M

V

G

G

L

L

R

R

E

K

S

D

D

D

P

T

E

E

L

L

R

K

A

A

K

A

F

F

D

D

A

K

A

A

I

L

Q

T

S

E

M

L

K

R

D

Q

D

D

G

G

S

T

L

Y

N

D

A

K

L

M

I

A

A

K

K

K

W

Y

L

F

binding arginine: D11 (≠ E32), Y14 (= Y35), F52 (≠ W73), S69 (≠ A90), S70 (≠ G91), S72 (= S93), R77 (= R98), L117 (≠ Q133), S120 (≠ T136), T121 (≠ I137), Q122 (= Q138), D161 (= D173)

5orgA Structure of the periplasmic binding protein (pbp) occj from a. Tumefaciens b6 in complex with octopine. (see paper)
32% identity, 87% coverage: 26:236/242 of query aligns to 5:250/257 of 5orgA

query
sites
5orgA

T

S

V

I

R

T

M

I

G

A

T

T

E
|
E

G

G

A

G

Y
|
Y

P

A

P

P

Y

W

N

N

F

F

L

S

N

G

D

P

A

G

G

G

E

K

V

L

D

D

G

G

F

F

E

E

R

I

E

D

L

L

G

A

D

N

E

A

L

L

C

C

A

E

R

K

A

M

E

K

L

A

T

K

C

C

E

Q

W

I

V

V

T

A

N

Q

D

N

W
|
W

D

D

S

G

I

I

I

M

P

P

N

S

L

L

V

T

S

G

G

K

N

K

Y

Y

D

D

T

A

I

I

I

M

A
|
A

G
x
A

M
|
M

S
|
S

I

V

T

T

D

P

E

K

R
|
R

D

Q

E

E

V

V

I

I

D

G

F

F

T

S

Q

I

N

P

Y

Y

T

A

Q

A

P

G

-

I

D
x
N

P

G

S

F

A

A

F

V

M

M

G

G

L

D

S

S

E

K

D

L

V

A

D

E

L

M

E

P

G

G

-

L

-

G

-

E

-

T

-

Y

-

S

-

L

-

D

-

S

-

Q

-

A

-

D

-

A

-

K

-

A

-

I

-

A

-

D

-

I

-

S

G

S

V

F

I

L

A

N

A

G

Q

T

T

T

G

V

T

G

I

V

Q
|
Q

A

G

S

S

H
x
T

I
x
T

A

A

S

S

T

T

-

F

-

L

-

D

-

K

-

Y

-

F

-

K

-

G

G

S

A

V

T

D

L

I

I

K

E

E

F

Y

A

K

S

S

P

V

D

E

E

E

T

H

V

N

A

L

A

D

V

L

R

T

N

S

G

G

E

R

A

L

D

D

A

A

V

V

L

L

A

A

D
x
N

K

A

A

T

F

V

L

L

-

A

-

I

E

E

P

K

F

P

V

E

T

M

E

K

N

G

A

A

D

K

L

L

V

V

-

G

-

P

-

L

F

F

V

S

G

G

E

G

D

E

V

F

L

-

G

-

G

G

G

V

I

V

G

A

M

V

G

G

L

L

R

R

E

K

S

E

D

D

P

T

E

A

L

L

R

K

A

A

K

D

F

F

D

D

A

A

I

I

Q

K

S

A

M

A

K

S

D

E

D

D

G

G

S

T

L

I

N

K

A

T

L

L

I

S

A

L

K

K

W

W

L

F

binding octopine: E11 (= E32), Y14 (= Y35), W52 (= W73), A69 (= A90), A70 (≠ G91), M71 (= M92), S72 (= S93), R77 (= R98), N92 (≠ D112), Q140 (= Q138), T143 (≠ H141), T144 (≠ I142), N183 (≠ D173)

P0AEU0 Histidine-binding periplasmic protein; HBP from Escherichia coli (strain K12) (see 3 papers)
31% identity, 87% coverage: 26:236/242 of query aligns to 27:253/260 of P0AEU0

query
sites
P0AEU0

T

N

V

I

R

R

M

I

G

G

T

T

E

D

G

P

A

T

Y

Y

P

A

P

P

Y

F

N

E

F

S

L

K

N

N

D

S

A

Q

G

G

E

E

V

L

D

V

G

G

F

F

E

D

R

I

E

D

L

L

G

A

D

K

E

E

L

L

C
|
C

A

K

R

R

A

I

E

N

L

T

T

Q

C
|
C

E

T

W

F

V

V

T

E

N

N

D

P

W

L

D

D

S

A

I

L

I

I

P

P

N

S

L

L

V

K

S

A

G

K

N

K

Y

I

D

D

T

A

I

I

I

M

A
x
S

G
x
S

M

L

S
|
S

I

I

T

T

D

E

E

K

R
|
R

D

Q

E

Q

V

E

I

I

D

A

F

F

T

T

Q

D

N

K

Y

L

T

Y

Q

A

P

A

D

D

P

S

S

R

A

L

F

V

M

V

G

A

L

K

S

N

E

S

D

D

V

I

D

Q

-

P

-

T

-

V

-

E

-

S

L

L

E

K

G

G

G

K

V

R

I

V

A

G

A

V

Q

L

T

Q

G

G

T

T

I
x
T

Q

Q

A

E

S

T

-

F

-

G

-

N

-

E

H

H

I

W

A

A

S

P

T

K

G

G

A

I

T

E

L

I

I

V

E

S

F

Y

A

Q

S

G

P

Q

D

D

E

N

T

I

V

Y

A

S

A

D

V

L

R

T

N

A

G

G

E

R

A

I

D

D

A

A

V

A

L

F

A

Q

D
|
D

K

E

-

V

A

A

F

A

L

S

E

E

P

G

F

F

V

L

T

K

E

Q

-

P

-

V

N

G

A

K

D

D

L

Y

V

K

F

F

V

G

G

G

E

P

D

S

V

V

-

K

-

D

-

E

-

K

L

L

L

F

G

G

G

V

G

G

I

T

G

G

M

M

G

G

L

L

R

R

E

K

S

E

D

D

P

N

E

E

L

L

R

R

A

E

K

A

F

L

D

N

A

K

A

A

I

F

Q

A

S

E

M

M

K

R

D

A

D

D

G

G

S

T

L

Y

N

E

A

K

L

L

I

A

A

K

K

K

W

Y

L

F

C60 (= C59) modified: Disulfide link with 67
C67 (= C66) modified: Disulfide link with 60
S91 (≠ A90) binding
S92 (≠ G91) binding
S94 (= S93) binding
R99 (= R98) binding
T143 (≠ I137) binding
D183 (= D173) binding

Sites not aligning to the query:

1:22 signal peptide

P02910 Histidine-binding periplasmic protein; HBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
31% identity, 87% coverage: 27:236/242 of query aligns to 28:253/260 of P02910

query
sites
P02910

V

I

R

R

M

I

G

G

T

T

E

D

G

P

A

T

Y

Y

P

A

P

P

Y

F

N
x
E

F

S

L
x
K

N

N

D

A

A

Q

G

G

E
|
E

V

L

D

V

G

G

F

F

E

D

R

I

E

D

L

L

G

A

D

K

E

E

L

L

C

C

A

K

R

R

A

I

E

N

L

T

T

Q

C

C

E

T

W

F

V

V

T

E

N

N

D

P

W

L

D

D

S

A

I

L

I

I

P

P

N

S

L

L

V

K

S

A

G

K

N

K

Y

I

D

D

T

A

I

I

I

M

A

S

G

S

M

L

S

S

I

I

T

T

D

E

E

K

R

R

D

Q

E

Q

V

E

I

I

D

A

F

F

T

T

Q

D

N

K

Y

L

T

Y

Q

A

P

A

D

D

P

S

S

R

A

L

F

V

M

V

G

A

L

K

S

N

E

S

D

D

V

I

D

Q

-

P

-

T

-

V

-

A

-

S

L

L

E

K

G

G

G

K

V

R

I

V

A

G

A

V

Q

L

T

Q

G

G

T

T

I

T

Q

Q

A

E

S

T

-

F

-

G

-

N

-

E

H

H

I

W

A

A

S

P

T

K

G

G

A

I

T

E

L

I

I

V

E

S

F

Y

A

Q

S

G

P

Q

D

D

E

N

T

I

V

Y

A

S

A
x
D

V

L

R

T

N

A

G

G

E
x
R

A

I

D
|
D

A

A

V

A

L

F

A

Q

D

D

K

E

-

V

A

A

F

A

L

S

E

E

P

G

F

F

V

L

T

K

E

Q

-

P

-

V

N

G

A

K

D

D

L

Y

V

K

F

F

-

G

-

P

-

A

V

V

G

K

E

D

D

E

V

K

L

L

L

F

G

G

G

V

G

G

I

T

G

G

M

M

G

G

L

L

R

R

E

K

S

E

D

D

P

N

E

E

L

L

R

R

A

E

K

A

F

L

D

N

A

K

A

A

I

F

Q

A

S

E

M

M

K

R

D

A

D

D

G

G

S

T

L

Y

N

E

A

K

L

L

I

A

A

K

K

K

W

Y

L

F

E40 (≠ N39) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
K42 (≠ L41) mutation to E: Increases ATPase-inducing activity and histidine transport; when associated with K-47.
E47 (= E46) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
D171 (≠ A161) mutation D->A,N: Strong decrease in ATPase-inducing activity and histidine transport.
R176 (≠ E166) mutation R->D,S: Strong decrease in ATPase-inducing activity and histidine transport.
D178 (= D168) mutation to A: Slight decrease in ATPase-inducing activity and histidine transport.

Sites not aligning to the query:

1:22 signal peptide

1hslA Refined 1.89 angstroms structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport(slash)chemosensory receptors (see paper)
31% identity, 87% coverage: 27:236/242 of query aligns to 6:231/238 of 1hslA

query
sites
1hslA

V

I

R

R

M

I

G

G

T

T

E

D

G

P

A

T

Y
|
Y

P

A

P

P

Y

F

N

E

F

S

L

K

N

N

D

A

A

Q

G

G

E

E

V

L

D

V

G

G

F

F

E

D

R

I

E

D

L

L

G

A

D

K

E

E

L

L

C

C

A

K

R

R

A

I

E

N

L

T

T

Q

C

C

E

T

W

F

V

V

T

E

N

N

D

P

W
x
L

D

D

S

A

I

L

I

I

P

P

N

S

L

L

V

K

S

A

G

K

N

K

Y

I

D

D

T

A

I

I

I

M

A
x
S

G
x
S

M
x
L

S
|
S

I

I

T

T

D

E

E

K

R
|
R

D

Q

E

Q

V

E

I

I

D

A

F

F

T

T

Q

D

N

K

Y

L

T

Y

Q

A

P

A

D

D

P

S

S

R

A

L

F

V

M

V

G

A

L

K

S

N

E

S

D

D

V

I

D

Q

-

P

-

T

-

V

-

A

-

S

L

L

E

K

G

G

G

K

V

R

I

V

A

G

A

V

Q

L

T

Q

G

G

T
|
T

I
x
T

Q

Q

A

E

S

T

-

F

-

G

-

N

-

E

H

H

I

W

A

A

S

P

T

K

G

G

A

I

T

E

L

I

I

V

E

S

F

Y

A

Q

S

G

P

Q

D

D

E

N

T

I

V

Y

A

S

A

D

V

L

R

T

N

A

G

G

E

R

A

I

D

D

A

A

V

A

L

F

A

Q

D
|
D

K

E

-

V

A

A

F

A

L

S

E

E

P

G

F

F

V

L

T

K

E

Q

-

P

-

V

N

G

A

K

D

D

L

Y

V

K

F

F

-

G

-

P

-

A

V

V

G

K

E

D