SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3608834 Dshi_2226 Glutamate--putrescine ligase (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
36% identity, 98% coverage: 7:455/456 of query aligns to 17:471/472 of P78061

query
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P78061

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H282 (= H266) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R342) mutation to Q: Activity is impaired to 3% of wild-type.

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
32% identity, 84% coverage: 70:453/456 of query aligns to 50:444/446 of A0R083

query
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A0R083

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K363 (≠ A381) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
31% identity, 84% coverage: 70:454/456 of query aligns to 50:446/446 of P9WN37

query
sites
P9WN37

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K363 (≠ A381) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

5dm3C Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
28% identity, 95% coverage: 18:448/456 of query aligns to 6:390/396 of 5dm3C

query
sites
5dm3C

R

K

A

T

Y

R

L

V

E

E

G

S

R

G

R

E

L

I

D

D

E

T

V

V

E

L

C

V

I

C

V

I

A

V

D

D

L

M

A

Q

G

G

V

R

A

L

R

M

G

G

K

K

A

R

M

L

P

H

A

A

A

R

K

H

F

F

A

-

R

-

Q

-

M

-

S

-

F

-

Y

-

L

-

P

-

N

-

S

-

I

-

F

-

Q

-

T

-

I

V

T

D

G

H

G

G

W

W

A

E

E

E

A
x
T

A
x
H

L

C

D

C

G

N

F

Y

T

L

E

L

P

-

D

-

M

Y

V

I

L

M

K

K

P

P

D

D

F

L

S

A

T

T

A

L

T

R

P

C

A

V

P

P

W

W

T

L

A

E

D

G

W

T

T

A

L

M

Q

V

I

L

I

C

H

D

D

L

I

L

E

D

D

H

Q

R

S

T

G

H

A

A

P

E

M

V

P

P

V

H

A

A

P

P

R

R

N

A

V

I

L

L

K

K

R

R

V

Q

I

L

A

A

L

R

Y

L

K

E

A

A

R

M

G

G

W

L

K

E

P

A

V
x
I

V

M

A
|
A

P

T

E
|
E

M

L

E
|
E

F

F

Y

F

L

L

T

F

A

E

R

K

N

S

I

L

D

D

P

E

A

I

I

-

A

-

I

-

Q

-

P

-

M

-

G

-

R

R

T

K

G

G

R

R

R

F

A

R

A

T

R

K

Q

E

A

E

Y

H

S

-

M

-

S

-

A

-

V

-

D

-

E

-

Y

-

G

-

P

-

V

V

I

L

D

R

D

P

I

L

Y

R

D

N

F

H

A

L

E

H

A

A

Q

A

G

G

L

I

E

P

I

V

D
x
E

G

G

I

T

T

K

Q

G

E
|
E

G

A

G

G

A

A

G

G

Q

Q

I

E

E
|
E

I

L

N

N

L

I

R
|
R

H
x
C

G

A

D

K

P

A

L

L

K

D

L

T

A

A

D

D

E

Y

V

H

F

T

Y

I

F

A

K

K

R

H

L

A

I

T

R

K

E

E

A

I

A

A

L

W

R

Q

H

Q

D

G

C

R

F

A

A

V

T

T

F

F

M

L

A

S

K

K

P

W

I

H

Q

H

D

A

E

H

P

A

G

G

S

S

A

S

M

S

H
|
H

I

I

H
|
H

H
x
Q

S
|
S

V

L

T
x
W

D

K

I

Q

A

G

T

L

G

-

E

P

N

A

I

F

F

H

T

D

A

E

P

R

D

D

G

A

T

L

E

G

T

M

P

S

A

A

F

L

-

M

Q

K

H

H

F

Y

I

L

A

A

G

G

L

L

Q

L

S

K

H

Y

L

A

G

P

D

D

V

Y

V

T

P

Y

L

F

Y

L

A

A

P

P

Y

Y

V

L

N

N

S

S

Y

Y

R

K

R

R

Y

F

V

-

P

-

E

-

N

Q

A

F

A

A

P

P

I

T

N

R

L

T

E

V

W

W

A

S

R

V

D

D

N

N

R
|
R

T

T

A

A

G

G

L

F

R

R

V

L

P

C

V

A

A

E

N

G

P

T

A

R

A
|
A

R

V

R
|
R

V

I

E
|
E

N

C

R
|
R

L

I

A

G

G

G

M

S

D

D

C

L

N

N

P

P

Y

Y

L

L

G

A

I

M

A

A

A

G

S

Q

L

L

A

A

C

A

G

G

Y

I

L

K

G

G

L

I

-

E

-

C

L

L

A

A

E

L

K

P

P

P

K

-

P

-

Q

-

Y

-

V

-

G

-

D

-

A

-

Y

-

S

-

G

-

S

A

E

S

D

G

L

L

A

I

P

P

E

Q

-

N

L

L

G

R

A

D

A

A

L

M

D

E

L

A

F

L

S

R

E

G

A

S

T

T

A

M

V

L

H

R

D

E

I

A

L

M

G

G

P

E

E

D

F

V

C

V

R

D

V

H

Y

Y

E

V

I

R

V

A

K

A

R

E

H

V

E

E

Y

L

T

E

E

D

F

F

L

Q

Q

R

V

V

I

V

S

S

P

D

W

Y

E

E

active site: E115 (= E145), E117 (= E147), E162 (= E210), E169 (= E217), H218 (= H266), R286 (= R337), E303 (= E354), R305 (= R356)
binding adenosine-5'-diphosphate: T46 (≠ A74), H47 (≠ A75), I111 (≠ V141), A113 (= A143), E157 (≠ D205), R173 (= R221), C174 (≠ H222), H220 (= H268), Q221 (≠ H269), S222 (= S270), W224 (≠ T272), A299 (= A350), R301 (= R352)

7tfaB Glutamine synthetase (see paper)
28% identity, 92% coverage: 34:453/456 of query aligns to 24:438/441 of 7tfaB

query
sites
7tfaB

A

T

D

D

L

L

A

L

G

G

V

T

A

I

R

K

G

N

K

V

A

E

M

I

P

P

A

V

A

S

K

Q

F

L

A

E

R

K

Q

A

M

-

S

-

F

-

Y

-

L

L

P

D

N

N

S

K

I

M

F

M

F

F

Q

D

T

G

I

-

T

-

G

-

W

-

A

-

E

-

A

S

L

I

D

E

G

G

F
x
Y

T

V

-
x
R

-

I

-

E

E

E

P

S

D

D

M

M

V

Y

L

L

K

Y

P

P

D

D

F

L

S

D

T

T

A

W

T

V

P

V

A

F

P

P

W

W

-

V

T

T

A

S

D

D

W

R

T

V

L

A

Q

R

I

L

I

I

H

C

D

D

I

I

E

Y

D

K

Q

P

S

D

G

G

A

S

P

P

M

F

P

A

V

G

A

D

P

P

R

R

N

G

V

I

L

L

K

K

R

R

V

V

I

L

A

K

L

E

Y

A

K

E

A

E

R

L

G

G

W

Y

K

T

P

S

V

M

-

N

V

V

A

G

P

P

E
|
E

M

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

T

T

A

D

R

E

N

K

I

G

D

D

P

P

A

T

I

T

A

E

I

L

Q

N

P

D

P

Q

M

G

G

G

R

-

T

-

G

-

R

-

A

-

R

-

Q

-

A
x
Y

Y

F

S

D

M

L

S

A

A

P

V

M

D

D

E

L

Y

G

G

E

P

N

V

C

I

R

D

R

D

E

I

I

Y

V

D

L

F

K

A

L

E

E

A

E

Q

M

G

G

L

F

E

E

I

I

D

E

G

A

I

S

T

H

Q

H

E
|
E

G
x
V

G

A

A

P

G

G

Q

Q

I

H

E
|
E

I

I

N

D

L

F

R

K

H

Y

G

A

D

D

P

A

L

V

K

K

L

A

A

A

D

D

E

Q

V

I

F

Q

Y

T

F

F

K

K

R

L

L

V

I

V

R

K

E

T

A

I

A

A

L

R

R

Q

H

H

D

G

C

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

Q

F

D
x
G

E

V

P
x
N

G
|
G

S
|
S

A
x
G

M

M

H
|
H

I

C

H

N

H

Q

S

S

V

L

T

-

D

-

I

F

A

K

T

D

G

N

E

E

N

N

I

V

F

F

-

Y

-

D

-

E

T

T

A

D

P

E

D

L

G

G

T

L

E

S

T

Q

P

T

A

A

F

-

Q

R

H

H

F

Y

I

M

A

A

G

G

L

I

Q

L

S

K

H

H

L

A

G

R

D

A

V

M

V

A

P

A

L

I

Y

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

P

P

E
x
G

N
x
Y

A
x
E

A

A

P

P

I

C

N

Y

L

V

E

A

W

W

A

S

R

A

D

S

N

N

R
|
R

T

S

A

P

G

M

L

I

R

R

V

I

P

P

V

A

A

S

N

R

P

G

A

L

A

S

R

T

R

R

V

V

E
|
E

N

V

R

R

L

N

A

P

G

D

M

P

D

A

C

A

N

N

P

P

Y

Y

L

L

G

A

I

L

A

A

A

V

S

M

L

L

A

R

C

A

G

G

Y

L

L

D

G

G

L

I

L

K

A

R

E

Q

K

M

P

A

P

L

K

P

P

A

Q

P

Y

I

V

D

G

R

D

N

A

I

Y

Y

S

V

G

M

S

S

E

E

D

E

-

E

-

R

-

I

-

E

-

G

-

I

-

P

-

S

L

L

A

P

P

A

E

D

L

L

G

K

A

E

A

A

L

L

D

S

L

E

F

L

S

I

E

R

A

S

T

E

A

V

V

I

H

S

D

D

I

A

L

L

G

G

P

D

E

H

F

A

C

L

R

A

V

Y

Y

F

E

Y

I

E

V

L

K

K

R

E

H
x
I

E

E

Y

W

T

D

E
x
M

F

Y

L

R

Q

T

V

Q

I

V

S

H

P

Q

W
|
W

E

E

R

R

E

D

H
x
Q

L

Y

L

L

binding glutamine: E131 (= E147), Y153 (≠ A177), E186 (= E210), N237 (≠ P261), G238 (= G262), S239 (= S263), G240 (≠ A264), H242 (= H266), R295 (= R319), E301 (≠ A325)
binding magnesium ion: E129 (= E145), E131 (= E147), E186 (= E210), E193 (= E217), H242 (= H266), E330 (= E354)
binding : Y58 (≠ F79), R60 (vs. gap), V187 (≠ G211), G235 (≠ D259), N237 (≠ P261), G299 (≠ E323), Y300 (≠ N324), R313 (= R337), I420 (≠ H435), M424 (≠ E439), W432 (= W447), Q436 (≠ H451)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
28% identity, 92% coverage: 34:453/456 of query aligns to 25:440/443 of 4lnkA

query
sites
4lnkA

A

T

D

D

L

I

A

L

G

G

V

T

A

I

R

K

G

N

K

V

A

E

M

I

P

P

A

V

A

S

K

Q

F

L

A

G

R
x
K

Q

A

M

-

S

-

F

-

Y

-

L

L

P

D

N

N

S

K

I

V

F
x
M

F

F

Q
x
D

T

G

I

-

T

-

G

-

W

-

A

-

E

-

A

S

L

I

D

E

G

G

F

F

T

V

-

R

-

I

-

E

E

E

P

S

D

D

M

M

V

Y

L

L

K

Y

P

P

D

D

F

L

S

N

T

T

A

F

T

V

P

I

A

F

P

P

W

W

T

T

A

A

D

E

W

K

-

G

-

K

T

V

L

A

Q

R

I

F

I

I

H

C

D

D

I

I

E

Y

D

N

Q

P

S

D

G

G

A

T

P

P

M

F

P

E

V

G

A

D

P

P

R

R

N

N

V

N

L

L

K

K

R

R

V

I

I

L

A

K

L

E

Y

M

K

E

A

D

R

L

G

G

W

F

K

S

P

D

V

F

-
x
N

V

L

A
x
G

P

P

E
|
E

M

P

E
|
E

F

F

Y

F

L

L

T

F

A

K

-

L

-

D

R

E

N

K

I

G

D

E

P

P

A

T

I

L

A

E

I

L

Q

N

P

D

P

K

M

G

G

G

R

-

T

-

G

-

R

-

A

-

R

-

Q

-

A
x
Y

Y

F

S

D

M

L

S

A

A

P

V

T

D

D

E

L

Y

G

G

E

P

N

V

C

I

R

D

R

D

D

I

I

Y

V

D

L

F

E

A

L

E

E

A

E

Q

M

G

G

L

F

E

E

I

I

D

E

G

A

I

S

T

H

Q

H

E
|
E

G
x
V

G

A

A

P

G

G

Q

Q

I

H

E
|
E

I

I

N

D

L
x
F

R
x
K

H
x
Y

G

A

D

G

P

A

L

V

K

R

L

S

A

C

D

D

E

D

V

I

F

Q

Y

T

F

F

K

K

R

L

L

V

I

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

D

G

C

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

Q

F

D

G

E

V

P
x
N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

C

H
x
N

H
x
L

S
|
S

V

L

T

-

D

-

I
x
F

A

K

T

N

G

G

E

V

N

N

I

A

F

F

-

F

-

D

T

E

A

N

P

A

D

D

G

L

T

Q

E

L

T

S

P

E

A

T

F

A

Q

K

H

H

F

F

I

I

A

A

G

G

L

I

Q

V

S

K

H

H

L

A

G

T

D

S

V

F

V

T

P

A

L

V

Y

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

P

P

E

G

N

Y

A
x
E

A

A

P

P

I

C

N

Y

L

V

E

A

W

W

A

S

R

A

D

Q

N

N

R
|
R

T

S

A

P

G

L

L

I

R

R

V

I

P

P

V

A

A
x
S

N

R

P

G

A
x
I

A
x
S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

L

S

A

V

G

D

M

P

D

A

C

A

N

N

P

P

Y

Y

L

L

G

A

I

L

A

S

A

V

S

L

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

L

I

L

K

A

N

E

K

-

L

K

E

P

A

P

P

K

A

P

P

-

I

-

D

-

R

-

N

Q

I

Y

Y

V

V

G

M

D

S

A

K

-

E

-

R

-

M

Y

E

S

N

G

G

S

I

E

V

D

D

L

L

A

P

P

A

E

T

L

L

G

A

A

E

A

A

L

L

D

E

L

E

F

F

S

K

E

S

A

N

T

E

A

V

V

M

H

V

D

K

I

A

L

L

G

G

P

E

E

H

F

L

C

F

R

E

V

H

Y

F

E

I

I

E

V

A

K

K

R

E

H

I

E

E

Y

W

T

D

E

M

F

F

L

R

Q

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

M

active site: D52 (≠ Q65), E131 (= E145), E133 (= E147), E188 (= E210), E195 (= E217), H244 (= H266), R315 (= R337), E332 (= E354), R334 (= R356)
binding adenosine-5'-diphosphate: K43 (≠ R52), M50 (≠ F63), N127 (vs. gap), G129 (≠ A143), E131 (= E145), F198 (≠ L220), K199 (≠ R221), Y200 (≠ H222), N246 (≠ H268), L247 (≠ H269), S248 (= S270), F250 (≠ I274), S324 (≠ A346), I327 (≠ A349), S328 (≠ A350), R330 (= R352)
binding glutamic acid: E133 (= E147), Y155 (≠ A177), E188 (= E210), V189 (≠ G211), N239 (≠ P261), G240 (= G262), G242 (≠ A264), H244 (= H266), R297 (= R319), E303 (≠ A325), R334 (= R356)
binding magnesium ion: E131 (= E145), E188 (= E210), E195 (= E217), H244 (= H266), E332 (= E354)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
28% identity, 92% coverage: 34:453/456 of query aligns to 25:440/443 of 4lniA

query
sites
4lniA

A

T

D

D

L

I

A

L

G

G

V

T

A

I

R

K

G

N

K

V

A

E

M

I

P

P

A

V

A

S

K

Q

F

L

A

G

R

K

Q

A

M

-

S

-

F

-

Y

-

L

L

P

D

N

N

S

K

I

V

F

M

F

F

Q
x
D

T

G

I

-

T

-

G

-

W

-

A

-

E

-

A

S

L

I

D

E

G

G

F

F

T

V

-

R

-

I

-

E

E

E

P

S

D

D

M

M

V

Y

L

L

K

Y

P

P

D

D

F

L

S

N

T

T

A

F

T

V

P

I

A

F

P

P

W

W

T

T

A

A

D

E

W

K

-

G

-

K

T

V

L

A

Q

R

I

F

I

I

H

C

D

D

I

I

E

Y

D

N

Q

P

S

D

G

G

A

T

P

P

M

F

P

E

V

G

A

D

P

P

R

R

N

N

V

N

L

L

K

K

R

R

V

I

I

L

A

K

L

E

Y

M

K

E

A

D

R

L

G

G

W

F

K

S

P

D

V

F

-
x
N

V

L

A
x
G

P

P

E
|
E

M

P

E
|
E

F

F

Y

F

L

L

T

F

A

K

-

L

-

D

R

E

N

K

I

G

D

E

P

P

A

T

I

L

A

E

I

L

Q

N

P

D

P

K

M

G

G

G

R

-

T

-

G

-

R

-

A

-

R

-

Q

-

A
x
Y

Y

F

S

D

M

L

S

A

A

P

V

T

D

D

E

L

Y

G

G

E

P

N

V

C

I

R

D

R

D

D

I

I

Y

V

D

L

F

E

A

L

E

E

A

E

Q

M

G

G

L

F

E

E

I

I

D
x
E

G

A

I

S

T

H

Q

H

E
|
E

G

V

G

A

A

P

G

G

Q

Q

I

H

E
|
E

I

I

N
x
D

L
x
F

R
x
K

H
x
Y

G

A

D

G

P

A

L

V

K

R

L

S

A

C

D

D

E

D

V

I

F

Q

Y

T

F

F

K

K

R

L

L

V

I

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

D

G

C

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

Q

F

D

G

E

V

P

N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

C

H
x
N

H

L

S
|
S

V

L

T

-

D

-

I

F

A

K

T

N

G

G

E

V

N

N

I

A

F

F

-

F

-

D

T

E

A

N

P

A

D

D

G

L

T

Q

E

L

T

S

P

E

A

T

F

A

Q

K

H

H

F

F

I

I

A

A

G

G

L

I

Q

V

S

K

H

H

L

A

G

T

D

S

V

F

V

T

P

A

L

V

Y

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

P

P

E

G

N
x
Y

A
x
E

A
|
A

P

P

I

C

N

Y

L

V

E

A

W

W

A

S

R

A

D

Q

N

N

R
|
R

T

S

A

P

G

L

L

I

R
|
R

V

I

P

P

V

A

A

S

N

R

P

G

A
x
I

A
x
S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

L

S

A

V

G

D

M

P

D

A

C

A

N

N

P

P

Y

Y

L

L

G

A

I

L

A

S

A

V

S

L

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

L

I

L

K

A

N

E

K

-

L

K

E

P

A

P

P

K

A

P

P

-

I

-

D

-

R

-

N

Q

I

Y

Y

V

V

G

M

D

S

A

K

-

E

-

R

-

M

Y

E

S

N

G

G

S

I

E

V

D

D

L

L

A

P

P

A

E

T

L

L

G

A

A

E

A

A

L

L

D

E

L

E

F

F

S

K

E

S

A

N

T

E

A

V

V

M

H

V

D

K

I

A

L

L

G

G

P

E

E

H

F

L

C

F

R

E

V

H

Y

F

E

I

I

E

V

A

K

K

R

E

H

I

E

E

Y

W

T

D

E

M

F

F

L

R

Q

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

M

active site: D52 (≠ Q65), E131 (= E145), E133 (= E147), E188 (= E210), E195 (= E217), H244 (= H266), R315 (= R337), E332 (= E354), R334 (= R356)
binding adenosine-5'-diphosphate: N127 (vs. gap), G129 (≠ A143), E131 (= E145), E183 (≠ D205), D197 (≠ N219), F198 (≠ L220), K199 (≠ R221), Y200 (≠ H222), N246 (≠ H268), S248 (= S270), R315 (= R337), R320 (= R342), I327 (≠ A349), S328 (≠ A350), R330 (= R352), E332 (= E354)
binding magnesium ion: E131 (= E145), E131 (= E145), E133 (= E147), E188 (= E210), E195 (= E217), E195 (= E217), H244 (= H266), E332 (= E354)
binding l-methionine-s-sulfoximine phosphate: E131 (= E145), E133 (= E147), Y155 (≠ A177), E188 (= E210), E195 (= E217), G240 (= G262), G242 (≠ A264), H244 (= H266), R297 (= R319), Y302 (≠ N324), E303 (≠ A325), A304 (= A326), R315 (= R337), R334 (= R356)

4s0rD Structure of gs-tnra complex (see paper)
28% identity, 92% coverage: 34:453/456 of query aligns to 29:444/447 of 4s0rD

query
sites
4s0rD

A

T

D

D

L

I

A

L

G

G

V

T

A

I

R

K

G

N

K

V

A

E

M

I

P

P

A

V

A

S

K

Q

F

L

A

G

R

K

Q

A

M

-

S

-

F

-

Y

-

L

L

P

D

N

N

S

K

I

V

F

M

F

F

Q
x
D

T

G

I

-

T

-

G

-

W

-

A

-

E

-

A

S

L

I

D

E

G

G

F
|
F

T
x
V

-
x
R

-
x
I

-

E

E

E

P

S

D

D

M

M

V

Y

L

L

K

Y

P

P

D

D

F

L

S

N

T

T

A

F

T

V

P

I

A

F

P

P

W

W

T

T

A

A

D

E

W

K

-

G

-

K

T

V

L

A

Q

R

I

F

I

I

H

C

D

D

I

I

E

Y

D

N

Q

P

S

D

G

G

A

T

P

P

M

F

P

E

V

G

A

D

P

P

R

R

N

N

V

N

L

L

K

K

R

R

V

I

I

L

A

K

L

E

Y

M

K

E

A

D

R

L

G

G

W

F

K

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

M

P

E
|
E

F

F

Y

F

L

L

T

F

A

K

-

L

-

D

R

E

N

K

I

G

D

E

P

P

A

T

I

L

A

E

I

L

Q

N

P

D

P

K

M

G

G

G

R

-

T

-

G

-

R

-

A

-

R

-

Q

-

A

Y

Y

F

S
x
D

M

L

S

A

A

P

V

T

D

D

E

L

Y

G

G

E

P

N

V

C

I

R

D

R

D

D

I

I

Y

V

D

L

F

E

A

L

E

E

A

E

Q

M

G

G

L

F

E

E

I

I

D

E

G

A

I

S

T

H

Q

H

E
|
E

G
x
V

G
x
A

A
x
P

G

G

Q

Q

I

H

E
|
E

I

I

N

D

L

F

R

K

H

Y

G

A

D

G

P

A

L

V

K

R

L

S

A

C

D

D

E

D

V

I

F

Q

Y

T

F

F

K

K

R

L

L

V

I

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

D

G

C

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

Q

F

D
x
G

E
x
V

P
x
N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

C

H

N

H

L

S

S

V

L

T

-

D

-

I

F

A

K

T

N

G

G

E

V

N

N

I

A

F

F

-

F

-

D

T

E

A

N

P

A

D

D

G

L

T

Q

E

L

T

S

P

E

A

T

F

A

Q

K

H

H

F

F

I

I

A

A

G

G

L

I

Q

V

S

K

H

H

L

A

G

T

D

S

V

F

V

T

P

A

L

V

Y

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

P

P

E
x
G

N
x
Y

A
x
E

A
|
A

P

P

I

C

N

Y

L

V

E

A

W

W

A

S

R

A

D

Q

N

N

R
|
R

T

S

A

P

G

L

L

I

R

R

V

I

P

P

V

A

A

S

N

R

P

G

A

I

A

S

R

T

R

R

V

V

E
|
E

N

V

R
|
R

L

S

A

V

G

D

M

P

D

A

C

A

N

N

P

P

Y

Y

L

L

G

A

I

L

A

S

A

V

S

L

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

L

I

L

K

A

N

E

K

-

L

K

E

P

A

P

P

K

A

P

P

-

I

-

D

-

R

-
x
N

Q

I

Y
|
Y

V

V

G
x
M

D

S

A

K

-

E

-

R

-

M

Y

E

S

N

G

G

S

I

E

V

D

D

L

L

A

P

P

A

E

T

L

L

G

A

A

E

A

A

L

L

D

E

L

E

F

F

S
x
K

E
x
S

A

N

T

E

A

V

V

M

H

V

D

K

I

A

L

L

G

G

P

E

E

H

F

L

C

F

R

E

V
x
H

Y

F

E

I

I

E

V

A

K

K

R

E

H
x
I

E

E

Y

W

T

D

E
x
M

F

F

L

R

Q

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H
x
Q

L

Y

L

M

active site: D56 (≠ Q65), E135 (= E145), E137 (= E147), E192 (= E210), E199 (= E217), H248 (= H266), R319 (= R337), E336 (= E354), R338 (= R356)
binding glutamine: E137 (= E147), E192 (= E210), E199 (= E217), G244 (= G262), G246 (≠ A264), R301 (= R319), Y306 (≠ N324), E307 (≠ A325), A308 (= A326)
binding magnesium ion: I66 (vs. gap), E135 (= E145), E135 (= E145), E199 (= E217), H248 (= H266), H248 (= H266), E336 (= E354), K403 (≠ S412), S404 (≠ E413), H419 (≠ V428)
binding : F63 (= F79), V64 (≠ T80), R65 (vs. gap), I66 (vs. gap), D161 (≠ S179), V193 (≠ G211), A194 (≠ G212), P195 (≠ A213), G241 (≠ D259), V242 (≠ E260), N243 (≠ P261), G305 (≠ E323), Y306 (≠ N324), R319 (= R337), N374 (vs. gap), Y376 (= Y389), M378 (≠ G391), I426 (≠ H435), M430 (≠ E439), Q442 (≠ H451)

Sites not aligning to the query:

binding : 446

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
28% identity, 92% coverage: 34:453/456 of query aligns to 26:441/444 of P12425

query
sites
P12425

A

T

D

D

L

I

A

L

G

G

V

T

A

I

R

K

G

N

K

V

A

E

M

I

P

P

A

V

A

S

K

Q

F

L

A

G

R

K

Q

A

M

-

S

-

F

-

Y

-

L

L

P

D

N

N

S

K

I

V

F

M

F

F

Q

D

T

G

I

-

T

-

G

-

W

-

A

-

E

-

A

S

L

I

D

E

G
|
G

F

F

T

V

-
x
R

-

I

-

E

E

E

P

S

D

D

M

M

V

Y

L

L

K

Y

P

P

D

D

F

L

S

N

T

T

A

F

T

V

P

I

A

F

P

P

W

W

T

T

A

A

D

E

W

K

-

G

-

K

T

V

L

A

Q

R

I

F

I

I

H

C

D

D

I

I

E

Y

D

N

Q

P

S

D

G

G

A

T

P

P

M

F

P

E

V

G

A

D

P

P

R

R

N

N

V

N

L

L

K

K

R

R

V

I

I

L

A

K

L

E

Y

M

K

E

A

D

R

L

G

G

W

F

K

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

M

P

E
|
E

F

F

Y

F

L

L

T

F

A

K

-

L

-

D

R

E

N

K

I

G

D

E

P

P

A

T

I

L

A

E

I

L

Q

N

P

D

P

K

M

G

G

G

R

-

T

-

G

-

R

-

A

-

R

-

Q

-

A

Y

Y

F

S

D

M

L

S

A

A

P

V

T

D

D

E

L

Y

G

G

E

P

N

V

C

I

R

D

R

D

D

I

I

Y

V

D

L

F

E

A

L

E

E

A

E

Q

M

G

G

L

F

E

E

I

I

D

E

G

A

I

S

T

H

Q

H

E
|
E

G
x
V

G

A

A

P

G

G

Q

Q

I

H

E
|
E

I

I

N

D

L

F

R

K

H

Y

G

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A

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M

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G59 (= G78) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (vs. gap) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E145) binding
E134 (= E147) binding
E189 (= E210) binding
V190 (≠ G211) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E217) binding
G241 (= G262) binding
H245 (= H266) binding
G302 (≠ E323) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ A325) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P327) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E354) binding
E424 (= E436) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
28% identity, 92% coverage: 34:453/456 of query aligns to 24:436/439 of 7tdpA

query
sites
7tdpA

A

T

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D

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binding adenosine-5'-diphosphate: N123 (vs. gap), V124 (= V142), G125 (≠ A143), E127 (= E145), E179 (≠ D205), E191 (= E217), D193 (≠ N219), F194 (≠ L220), K195 (≠ R221), Y196 (≠ H222), N242 (≠ H268), Q243 (≠ H269), S244 (= S270), R311 (= R337), R316 (= R342), L323 (≠ A349), S324 (≠ A350), T325 (≠ R351), R326 (= R352), E328 (= E354)
binding magnesium ion: E127 (= E145), E127 (= E145), E129 (= E147), E184 (= E210), E191 (= E217), E191 (= E217), D193 (≠ N219), H240 (= H266), E328 (= E354)
binding l-methionine-s-sulfoximine phosphate: E127 (= E145), E129 (= E147), E184 (= E210), E191 (= E217), N235 (≠ P261), G236 (= G262), G238 (≠ A264), H240 (= H266), R293 (= R319), E299 (≠ A325), A300 (= A326), R311 (= R337), R330 (= R356)

7tenA Glutamine synthetase (see paper)
28% identity, 92% coverage: 34:453/456 of query aligns to 24:439/442 of 7tenA

query
sites
7tenA

A

T

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D