SitesBLAST

Q46444 Quinohemoprotein alcohol dehydrogenase; QH-ADH; Alcohol dehydrogenase (azurin); PQQ-containing alcohol dehydrogenase; PQQ-dependent ADH; Quinohaemoprotein ethanol dehydrogenase type I; QH-EDHI; EC 1.1.9.1 from Comamonas testosteroni (Pseudomonas testosteroni) (see 3 papers)
27% identity, 79% coverage: 143:749/769 of query aligns to 51:571/708 of Q46444

query
sites
Q46444

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E101 (≠ Q197) binding
C147 (= C242) modified: Disulfide link with 148
C148 (vs. gap) modified: Disulfide link with 147
R153 (= R243) binding
T198 (= T312) binding
GA 214:215 (≠ MA 332:333) binding
E216 (≠ V334) binding
T274 (≠ N378) binding
N294 (= N398) binding
D339 (= D442) binding
K366 (= K470) binding
NW 425:426 (= NW 570:571) binding

Sites not aligning to the query:

1:31 signal peptide
575 binding
635 binding covalent
638 binding covalent
639 binding axial binding residue
678 binding axial binding residue

Q4W6G0 Quinohemoprotein alcohol dehydrogenase ADH-IIG; ADH IIG; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
26% identity, 84% coverage: 106:749/769 of query aligns to 2:567/718 of Q4W6G0

query
sites
Q4W6G0

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F
x
W

G
|
G

G
x
A

M
x
H

N
x
D

W
|
W

G
x
Q

T
x
P

G
x
M

T
x
S

I
x
Y

D
x
N

E
x
P

A
x
D

R
x
T

G
|
G

L
|
L

L
x
V

I
x
Y

V
x
I

N
x
P

D
x
A

T
x
H

R
x
I

V
x
M

A
x
S

H
x
A

L
x
Y

Q
x
E

L
x
H

I
|
I

P
|
P

R
x
E

Q

-

E

-

A

-

D

-

A

-

L

-

G

-

V

-

E

-

V

-

G

-

P

-

H

-

D

-

G

-

L

-

A
|
A

P
|
P

Q
x
K

D
x
R

G
x
N

A

-

P
|
P

F
|
F

G
x
K

A
x
S

M
|
M

R
x
Y

S
x
Q

N

-

F

-

S

-

F

-

V
x
L

G
|
G

V
x
L

P
x
R

C
x
T

Q
x
G

E
x
M

P
x
M

P
|
P

Y
x
E

G
|
G

T
x
A

M
x
E

T
x
G

A
x
L

I
x
L

D
x
E

L
x
M

A
|
A

D
x
K

R
x
S

S

-

I

-

L

-

W
|
W

Q
x
S

R

-

P

-

V

-

G
|
G

T
x
K

I
x
L

E
x
I

E
x
A

T
x
W

G
x
D

P
|
P

F
x
V

G
x
K

I
x
Q

R
x
Q

T
x
A

G
x
A

W
|
W

R
x
E

M
x
V

P
|
P

V
x
Y

G
x
V

L
x
T

P
x
I

L
x
F

V
x
N

G
|
G

P
x
T

I
x
L

S
|
S

T
|
T

A
|
A

S
x
G

G
x
N

L
|
L

T
x
V

F
|
F

Y

-

S
x
E

G
|
G

T
x
S

Q
x
A

D
|
D

Y
x
G

Y
x
R

L
x
V

R
x
I

A
|
A

F
x
Y

S
x
A

E
x
D

T
|
T

G
|
G

E
|
E

E
x
K

L
|
L

W
|
W

R
x
E

G
x
Q

A
x
P

L
x
A

P
x
A

L
x
S

G
|
G

S
x
V

Q
x
M

S
x
A

T
x
A

P
|
P

M
x
V

S
x
T

Y
|
Y

I

-

G
x
S

P
x
V

D
|
D

G
|
G

R
x
E

Q
|
Q

Y
|
Y

V
|
V

V
x
T

V
x
F

T
x
M

V
x
A

G
|
G

C138 (≠ Y247) modified: Disulfide link with 139
C139 (≠ Y248) modified: Disulfide link with 138
R144 (= R253) binding
T189 (= T312) binding
GA 205:206 (≠ MA 332:333) binding
E207 (≠ V334) binding
T264 (≠ N378) binding
N284 (= N398) binding
D329 (= D442) binding
K356 (= K470) binding
W415 (≠ F566) binding
DW 419:420 (≠ NW 570:571) binding

Sites not aligning to the query:

1:29 signal peptide
30:718 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH-IIG
635 binding covalent
638 binding covalent
639 binding axial binding residue
676 binding axial binding residue

1kv9A Structure at 1.9 a resolution of a quinohemoprotein alcohol dehydrogenase from pseudomonas putida hk5 (see paper)
26% identity, 80% coverage: 135:749/769 of query aligns to 1:521/664 of 1kv9A

query
sites
1kv9A

A

A

N

G

T

V

D

D

P

E

V

A

P

A

T

I

R

R

A

A

I

T

A

E

A

Q

E

A

S

G

P

G

N

E

W

W

T

L

H

S

Y

H

G

G

R

R

T

T

P

Y

T

A

G

E

T

Q

R

R

F

F

A

S

P

P

F

L

D

K

Q

Q

I

I

T

D

P

A

E

S

N

N

V

V

G

R

E

S

L

L

E

G

V

L

A

A

W

W

T

Y

F

M

N

D

H

L

G

D

D

N

A

-

P

-

S

-

G

-

T

T

G

R

Q

G

D

L

Q
x
E

N

A

T

T

P

P

L

L

Y

F

V

H

D

D

G

G

L

V

V

I

Y

Y

H

T

C

S

S

M

P

S

N

W

N

S

I

R

V

V

S

I

A

A

I

V

D

D

A

A

V

A

T

S

G

G

A

K

Q

E

V

L

W

W

Q

R

Y

Y

D

D

P

P

E

E

A

V

S

A

S

-

P

-

L

-

W

-

Q

-

R

K

C

V

R

K

G
x
A

V
x
R

T

T

Y

S

Y
x
C

E
x
C

P

D

T

A

P

V

R

N

E
x
R

G

G

A

V

P

A

A

L

D

W

G

G

T

D

C

-

A

-

R

K

I

V

V

Y

L

V

S

G

S

T

V

L

D

D

A

G

R

R

L

L

I

I

Q

A

L

L

D

D

A

A

R

K

T

T

G

G

A

K

L

-

C

-

T

-

F

-

G

-

D

-

G

-

A

A

V

I

D

W

L

S

T

Q

R

Q

N

T

M

T

G

D

E

P

V

A

R

K

P

P

G

-

F

-

Y

Y

F

S

P

I

T
|
T

S

G

A

A

P

P

T

R

V

V

M

V

G

K

D

G

R

K

I

V

I

I

L

I

G

G

G

-

W

-

V

-

W

-

D

N

G
|
G

M
x
G

A
|
A

V
x
E

D

Y

E

G

P

V

S

R

G

G

V

F

V

V

R

S

A

A

F

Y

D

D

G

A

R

D

T

T

G

G

E

K

I

L

A

A

W

W

A

R

W

F

D

Y

I

T

-

V

-

P

G

G

N

D

P

P

A

A

I

L

D

P

T

Y

V

E

P

H

P

P

E

E

-

L

-

R

-

E

-

A

-

K

-

T

-

W

-

Q

G

G

E

D

T

Q

Y

Y

T

W

R

K

-

L

-

G

G

G

T

G

P

G

N
x
T

V

V

W
|
W

S

D

T

S

P

M

A

A

F

Y

D

D

A

P

A

E

L

L

G

D

L

L

V

L

Y

Y

L

V

P

G

T

T

G

G

N
|
N

A

G

Q

S

P

P

-

W

D

N

F

R

Y

E

G

V

S

R

S

S

R

P

I

G

E

G

A

G

A

D

N

N

D

L

W

Y

N

L

S

S

I

I

V

L

A

A

L

I

D

R

V

P

E

D

T

T

G

G

K

K

V

L

V

A

W

W

S

H

F

Y

Q

Q

T

V

T

T

Y

P

R

G

D

D

I

S

W

W

D
|
D

Y

F

D

T

V

A

P

T

A

Q

Q

Q

P

I

M

T

L

L

Y

A

D

E

I

L

T

N

T

I

E

D

T

-

G

G

E

K

V

P

V

R

P

K

A

V

L

L

I

M

Q

Q

V

A

T

P

K
|
K

R

N

S

G

Q

F

T

F

F

Y

V

V

L

L

D

D

R

R

R

T

D

N

G

G

T

K

P

L

I

I

A

S

E

A

V

E

R

K

D

F

L

G

P

K

V

V

P

T

T

W

T

A

G

E

G

K

V

V

E

D

P

L

D

A

Y

T

L

G

S

R

P

P

V

V

Q

E

P

A

Y

P

S

G

V

V

D

R

M

Y

P

E

V

-

I

-

G

-

A

K

E

E

P

P

L

I

T

-

E

-

A

-

R

-

M

-

W

-

G

-

A

-

T

-

P

-

L

-

D

-

H

-

L

-

Y

-

C

-

R

-

I

-

R

-

F

-

R

-

Q

-

A

-

Y

-

V

-

G

-

D

-

F

-

T

-

P

-

M

-

S

-

E

-

Q

-

G

-

T

-

L

V

I

M

W

W

P

P

G

S

Y
x
P

F
|
F

G

G

G

A

M

H

N
|
N

W
|
W

G

H

T

S

G

M

T

S

I

F

D

N

E

P

A

G

R

T

G

G

L

L

L

V

I

-

V

-

N

-

D

-

T

-

R

-

V

-

A

-

H

-

L

-

Q

-

L

Y

I

I

P

P

R

Y

Q

Q

E

E

A

V

D

P

A

-

L

-

G

-

V

-

E

-

V

-

G

-

P

-

H

-

D

-

G

-

L

-

A

-

P

-

Q

-

D

-

G

-

A

-

P

-

F

-

G

G

A

V

M

Y

R

R

S

N

N

E

F

G

F

K

S

D

F

F

V

V

G

T

V

R

P

K

C

A

Q

F

E

N

P

T

P

A

Y

A

G

G

T

F

M

A

T

D

A

A

I

T

D

D

L

V

A

P

D

A

R

A

S

V

I

V

L

-

W

-

Q

-

R

-

P

-

V

S

G

G

T

A

I

L

E

L

E

A

T

W

G

D

P

P

F

V

G

K

I

Q

R

K

T

A

G

A

W

W

R

K

M

V

P

P

V

Y

G

P

L

T

P

H

L
x
W

V

N

G

G

P

T

I

L

S

S

T

T

A

A

S

G

G

N

L

L

T

V

F

F

Y

-

S

Q

G

G

T

T

Q

A

D

A

Y

G

Y

Q

L

M

R

H

A

A

F

Y

S

S

S

A

E

D

T

K

G

G

E

E

E

A

L

L

W

W

R

Q

G

F

A

E

L

A

P

Q

L

S

G

G

S

I

Q

V

S

A

T

A

P

P

M

M

S

T

Y

F

I

-

G

E

P

L

D

A

G

G

R

R

Q

Q

Y

Y

V

V

V

A

V

I

T

M

V

A

G

G

active site: E173 (≠ V334), N250 (= N398), D295 (= D442)
binding acetone: E173 (≠ V334), D295 (= D442), P377 (≠ Y565), F378 (= F566)
binding calcium ion: E173 (≠ V334), N250 (= N398), D295 (= D442)
binding heme c: A101 (≠ G244), R102 (≠ V245)
binding pyrroloquinoline quinone: E59 (≠ Q197), C105 (≠ Y248), C106 (≠ E249), R111 (≠ E254), T155 (= T312), G170 (= G331), G171 (≠ M332), A172 (= A333), E173 (≠ V334), T230 (≠ N378), W232 (= W380), N250 (= N398), D295 (= D442), K322 (= K470), N382 (= N570), W383 (= W571), W460 (≠ L686)

Sites not aligning to the query:

binding heme c: 590, 591, 594, 595, 605, 606, 608, 611, 615, 619, 622, 623, 631, 633, 634, 636
binding pyrroloquinoline quinone: 524, 525

1yiqA Molecular cloning and structural analysis of quinohemoprotein alcohol dehydrogenase adhiig from pseudomonas putida hk5. Compariison to the other quinohemoprotein alcohol dehydrogenase adhiib found in the same microorganism. (see paper)
26% identity, 80% coverage: 132:749/769 of query aligns to 2:538/684 of 1yiqA

query
sites
1yiqA

N

D

V

I

I

P

A

A

N

N

T

V

D

D

P

G

V

A

P

R

T

I

R

I

A

A

I

A

A

D

A

K

E

E

S

P

P

G

N

N

W

W

T

M

H

S

Y

T

G

G

R

R

T

T

P

Y

T

D

G

E

T

Q

R

R

F

Y

A

S

P

P

F

L

D

K

Q

Q

I

I

T

S

P

D

E

Q

N

N

V

V

G

G

E

Q

L

L

E

G

V

L

A

A

W

W

T

S

F

Y

N

K

H

L

G

-

D

D

A

L

P

D

S
x
R

G

G

T

V

G

-

Q

-

D

-

Q
x
E

N

A

T

T

P

P

L

I

Y

V

V

V

D

D

G

G

L

V

V

M

Y

Y

H

T

C

T

S

G

P

P

N

F

N

S

I

V

V

V

S

Y

A

A

I

L

D

D

A

A

V

R

T

D

G

G

A

R

Q

L

V

I

W

W

Q

K

Y

Y

D

D

P

P

E

Q

A

S

S

D

S

-

P

-

L

-

W

-

Q

R

R

H

C

R

R

A

G
|
G

V

E

T

A

Y
x
C

E

D

P

A

T

V

P

N

R
|
R

E

G

G

V

A

A

P

V

A

W

D

K

G

G

T

-

C

-

A

-

R

K

I

V

V

Y

L

V

S

G

S

V

V

L

D

D

A

G

R

R

L

L

I

E

Q

A

L

I

D

D

A

A

R

K

T

T

G

G

A

Q

L

R

C

A

T

W

T

S

F

V

G

D

D

T

G

-

A

R

V

A

D

D

L

H

T

K

R

R

N

S

M

-

G

-

E

-

V

-

R

-

P

-

G

-

F

-

Y

Y

F

T

P

I

T
|
T

S

G

A

A

P

P

T

R

V

V

M

V

G

N

D

G

R

K

I

V

L

I

G

G

G

-

W

-

V

-

W

-

D

N

G
|
G

M
x
G

A
|
A

V
x
E

D

F

E

G

P

V

S

R

G

G

V

Y

V

V

R

T

A

A

F

Y

D

D

G

A

R

E

T

T

G

G

E

K

I

E

A

A

W

W

A

R

W

F

D

-

I

Y

G

T

N

V

P

P

A

G

I

D

D

P

T

K

V

L

P

P

E

E

-

G

-

K

-

G

-

M

-

E

-

I

-

A

-

K

-

T

-

W

-

F

G

G

E

D

T

A

Y

Y

T

V

R

E

-

Q

-

G

G

G

T

G

P

G

N
x
T

V

A

W
|
W

S

D

T

S

P

F

A

A

F

Y

D

D

A

P

A

E

L

L

G

N

L

L

V

L

Y

Y

L

I

P

G

T

V

G

G

N
|
N

A

G

Q

S

P

L

-

W

D

D

F

P

Y

K

G

W

S

R

S

S

R

Q

I

A

E

K

A

G

A

D

N

N

D

L

W

F

N

L

S

S

I

I

V

V

A

A

L

V

D

N

V

A

E

D

T

T

G

G

K

E

V

Y

V

V

W

W

S

H

F

Y

Q

Q

T

T

Y

P

R

G

D

D

I

A

W

W

D
|
D

Y

Y

D

T

V

A

P

T

A

Q

Q

H

P

M

M

I

L

L

Y

A

D

E

I

L

T

P

T

I

E

D

T

-

G

G

E

K

V

P

V

R

P

K

A

V

L

L

I

M

Q

Q

V

A

T

P

K
|
K

R

N

S

G

Q

F

T

F

F

Y

V

V

L

I

D

D

R

R

R

A

D

T

G

G

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L

I

L

A

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A

V

K

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D

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L

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P

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W

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G

G

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M

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-

D

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Y

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L

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Y

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A

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A

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P

P

M

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S

T

Y

Y

I

-

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P

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G

active site: E178 (≠ V334), N255 (= N398), D300 (= D442)
binding calcium ion: E178 (≠ V334), N255 (= N398), D300 (= D442)
binding heme c: R60 (≠ S191), G106 (= G244)
binding pyrroloquinoline quinone: E63 (≠ Q197), C109 (≠ Y247), C110 (≠ Y248), R115 (= R253), T160 (= T312), G175 (= G331), G176 (≠ M332), A177 (= A333), E178 (≠ V334), T235 (≠ N378), W237 (= W380), N255 (= N398), D300 (= D442), K327 (= K470), W386 (≠ F566), D390 (≠ N570), W391 (= W571), F477 (≠ L686)

Sites not aligning to the query:

binding heme c: 605, 606, 608, 609, 610, 621, 623, 626, 630, 634, 637, 638, 642, 645, 646, 647, 648, 650, 654, 662
binding pyrroloquinoline quinone: 541, 542

Q8GR64 Quinohemoprotein alcohol dehydrogenase ADH IIB; ADH IIB; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
26% identity, 80% coverage: 138:749/769 of query aligns to 26:543/690 of Q8GR64

query
sites
Q8GR64

D
|
D

P
x
E

V
x
A

P
x
A

T
x
I

R
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R

A
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A

I
x
T

A
x
E

A
x
Q

E
x
A

S
x
G

P
x
G

N
x
E

W
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W

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x
L

H
x
S

Y
x
H

G
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G

R
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R

T
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T

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Y

T
x
A

G
x
E

T
x
Q

R
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R

F
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F

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x
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P

F
x
L

D
x
K

Q
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Q

I
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I

T
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D

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S

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N

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V

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L

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L

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A

W
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W

T
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Y

F
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M

N
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G
x
D

D
x
N

A

-

P

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S

-

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-

T
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T

G
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x
G

D
x
L

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x
E

N
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A

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T

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L

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H

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Y

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A

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I
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I

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x
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N

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L
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L

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L

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T

G
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G

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A
x
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P

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N

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