SitesBLAST

Q9HDV9 Putative glycine cleavage system H protein, mitochondrial; Glycine decarboxylase complex subunit H from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
50% identity, 98% coverage: 3:121/122 of query aligns to 44:162/169 of Q9HDV9

query
sites
Q9HDV9

T

T

T

K

Y

H

Y

F

T

T

D

K

D

E

H

H

E

E

W

W

I

V

E

K

V

V

E

D

D

G

D

D

T

V

A

G

T

T

I

V

G

G

I

I

T

T

K

S

H

Y

A

A

E

N

Q

A

L

L

G

G

E

E

V

V

F

F

I

V

E

E

L

L

Q

-

P

P

E

E

G

P

E

E

T

T

F

T

V

V

K

S

-

V

G

G

D

D

E

G

I

I

G

G

V

A

V

V

E

E

S

S

V

V

K

K

A

S

A

A

S

S

D

D

I

V

F

Y

A

S

P

P

V

V

T

S

G

G

E

T

I

V

L

T

E

S

A

I

N

N

A

E

A

S

L

L

V

G

E

D

T

S

P

P

A

D

E

K

L

V

N

S

E

S

D
x
S

P

P

E

E

G

E

N

E

S

G

W

W

L

I

Y

C

K

K

I

I

K

K

L

L

S

S

D

S

P

P

G

D

E

E

L

L

S

K

E

S

L

L

D

N

A

D

E

E

G

S

Y

Y

A

A

A

Q

L

F

I

C

S131 (≠ D90) modified: Phosphoserine

3ab9A Crystal structure of lipoylated e. Coli h-protein (reduced form) (see paper)
51% identity, 95% coverage: 6:121/122 of query aligns to 8:124/127 of 3ab9A

query
sites
3ab9A

Y

Y

T

S

D

K

D

E

H

H

E

E

W

W

I

L

E

R

V

K

E

E

-

A

D

D

D

G

T

T

A

Y

T

T

I

V

G

G

I

I

T

T

K

E

H

H

A

A

A

Q

E

E

Q

L

L

L

G

G

E

D

V

M

V

V

F

F

I

V

E
x
D

L
|
L

Q

P

P

E

E

V

G

G

E

A

T

T

F

V

V

S

K

A

G

G

D

D

E

D

I

C

G

A

V

V

V

A

E

E

S

S

V

V

K

K

A

A

S

S

D

D

I

I

F

Y

A

A

P

P

V

V

T

S

G

G

E

E

I

I

L

V

E

A

A

V

N

N

A

D

A

A

L

L

V

S

E

D

T

S

P

P

A

E

E

L

L

V

N

N

E

S

D

E

P

P

E

Y

G

A

N

G

S

G

W

W

L

I

Y

F

K

K

I

I

K

K

L

A

S

S

D

D

P

E

G

S

E

E

L

L

S

E

E

S

L

L

D

D

A

A

E

T

G

A

Y

Y

A

E

A

A

L

L

I

L

binding calcium ion: D42 (≠ E39), L43 (= L40)

P16048 Glycine cleavage system H protein, mitochondrial from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see paper)
46% identity, 95% coverage: 6:121/122 of query aligns to 43:158/165 of P16048

query
sites
P16048

Y

Y

T

A

D

P

D

S

H

H

E

E

W

W

I

V

E

K

V

H

E

E

D

G

D

S

T

V

A

A

T

T

I

I

G

G

I

I

T

T

K

D

H

H

A

A

A

Q

E

D

Q

H

L

L

G

G

E

E

V

V

F

F

I

V

E

E

L

L

Q

P

P

E

E

P

G

G

E

V

T

S

F

V

V

T

K

K

G

G

D

K

E

G

I

F

G

G

V

A

V

V

E

E

S

S

V

V

K
|
K

A

A

A

T

S

S

D

D

I

V

F

N

A

S

P

P

V

I

T

S

G

G

E

E

I

V

L

I

E

E

A

V

N

N

A

T

A

G

L

L

V

T

E

G

T

K

P

P

A

G

E

L

L

I

N

N

E

S

D

S

P

P

E

Y

G

E

N

D

S

G

W

W

L

M

Y

I

K

K

I

I

K

K

L

P

S

T

D

S

P

P

G

D

E

E

L

L

S

E

E

S

L

L

D

G

A

A

E

K

G

E

Y

Y

A

T

A

K

L

F

I

C

K97 (= K60) modified: N6-lipoyllysine

1htpA Refined structures at 2 angstroms and 2.2 angstroms of the two forms of the h-protein, a lipoamide-containing protein of the glycine decarboxylase complex (see paper)
46% identity, 95% coverage: 6:121/122 of query aligns to 9:124/131 of 1htpA

query
sites
1htpA

Y

Y

T

A

D

P

D
x
S

H
|
H

E
|
E

W

W

I

V

E

K

V

H

E

E

D

G

D

S

T

V

A

A

T

T

I

I

G

G

I

I

T

T

K

D

H

H

A

A

A

Q

E

D

Q
x
H

L

L

G

G

E

E

V

V

F

F

I

V

E

E

L

L

Q

P

P

E

E

P

G

G

E

V

T

S

F

V

V

T

K

K

G

G

D

K

E

G

I

F

G

G

V

A

V

V

E

E

S

S

V

V

K
|
K

A

A

A

T

S

S

D

D

I

V

F

N

A

S

P

P

V

I

T

S

G

G

E

E

I

V

L

I

E

E

A

V

N

N

A

T

A

G

L

L

V

T

E

G

T

K

P

P

A

G

E

L

L

I

N

N

E

S

D

S

P

P

E

Y

G

E

N

D

S

G

W

W

L

M

Y

I

K

K

I

I

K

K

L

P

S

T

D

S

P

P

G

D

E

E

L

L

S

E

E

S

L

L

D

G

A

A

E

K

G

E

Y

Y

A

T

A

K

L

F

I

C

binding 6-(hydroxyethyldithio)-8-(aminomethylthio)octanoic acid: S12 (≠ D9), H13 (= H10), E14 (= E11), H34 (≠ Q31), K63 (= K60)

Sites not aligning to the query:

binding 6-(hydroxyethyldithio)-8-(aminomethylthio)octanoic acid: 127

1hpcB Refined structures at 2 angstroms and 2.2 angstroms of the two forms of the h-protein, a lipoamide-containing protein of the glycine decarboxylase (see paper)
46% identity, 95% coverage: 6:121/122 of query aligns to 9:124/131 of 1hpcB

query
sites
1hpcB

Y

Y

T

A

D

P

D

S

H

H

E

E

W

W

I

V

E

K

V

H

E

E

D

G

D

S

T

V

A

A

T

T

I

I

G

G

I

I

T

T

K

D

H

H

A

A

A

Q

E

D

Q
x
H

L

L

G

G

E

E

V

V

F

F

I

V

E

E

L

L

Q

P

P

E

E

P

G

G

E

V

T

S

F

V

V

T

K

K

G

G

D

K

E

G

I

F

G

G

V

A

V

V

E

E

S

S

V

V

K
|
K

A

A

A

T

S

S

D

D

I

V

F

N

A

S

P

P

V

I

T

S

G

G

E

E

I

V

L

I

E

E

A

V

N

N

A

T

A

G

L

L

V

T

E

G

T

K

P

P

A

G

E

L

L

I

N

N

E

S

D

S

P

P

E

Y

G

E

N

D

S

G

W

W

L

M

Y

I

K

K

I

I

K

K

L

P

S

T

D

S

P

P

G

D

E

E

L

L

S

E

E

S

L

L

D

G

A

A

E

K

G

E

Y

Y

A

T

A

K

L

F

I

C

binding lipoic acid: H34 (≠ Q31), K63 (= K60)

1hpcA Refined structures at 2 angstroms and 2.2 angstroms of the two forms of the h-protein, a lipoamide-containing protein of the glycine decarboxylase (see paper)
46% identity, 95% coverage: 6:121/122 of query aligns to 9:124/131 of 1hpcA

query
sites
1hpcA

Y

Y

T

A

D

P

D

S

H

H

E

E

W

W

I

V

E

K

V

H

E

E

D

G

D

S

T

V

A

A

T

T

I

I

G

G

I

I

T

T

K

D

H

H

A

A

A

Q

E

D

Q
x
H

L

L

G

G

E

E

V

V

F

F

I

V

E

E

L

L

Q

P

P

E

E

P

G

G

E

V

T

S

F

V

V

T

K

K

G

G

D

K

E

G

I

F

G

G

V

A

V

V

E

E

S

S

V

V

K
|
K

A

A

A

T

S

S

D

D

I

V

F

N

A

S

P

P

V

I

T

S

G

G

E

E

I

V

L

I

E

E

A

V

N

N

A

T

A

G

L

L

V

T

E

G

T

K

P

P

A

G

E

L

L

I

N

N

E

S

D

S

P

P

E

Y

G

E

N

D

S

G

W

W

L

M

Y

I

K

K

I

I

K

K

L

P

S

T

D

S

P

P

G

D

E

E

L

L

S

E

E

S

L

L

D

G

A

A

E

K

G

E

Y

Y

A

T

A

K

L

F

I

C

binding 5-[(3S)-1,2-dithiolan-3-yl]pentanoic acid: H34 (≠ Q31), K63 (= K60)

1dxmA Reduced form of the h protein from glycine decarboxylase complex (see paper)
46% identity, 95% coverage: 6:121/122 of query aligns to 9:124/131 of 1dxmA

query
sites
1dxmA

Y

Y

T

A

D

P

D

S

H

H

E

E

W

W

I

V

E

K

V

H

E

E

D

G

D

S

T

V

A

A

T

T

I

I

G

G

I

I

T

T

K

D

H

H

A

A

A

Q

E

D

Q
x
H

L
|
L

G

G

E

E

V

V

F

F

I

V

E

E

L

L

Q

P

P

E

E

P

G

G

E

V

T

S

F

V

V

T

K

K

G

G

D

K

E

G

I

F

G

G

V

A

V

V

E

E

S

S

V

V

K
|
K

A

A

A

T

S

S

D

D

I

V

F

N

A

S

P

P

V

I

T

S

G

G

E

E

I

V

L

I

E

E

A

V

N

N

A

T

A

G

L

L

V

T

E

G

T

K

P

P

A

G

E

L

L

I

N

N

E

S

D

S

P

P

E

Y

G

E

N

D

S

G

W

W

L

M

Y

I

K

K

I

I

K

K

L

P

S

T

D

S

P

P

G

D

E

E

L

L

S

E

E

S

L

L

D

G

A

A

E

K

G

E

Y

Y

A

T

A

K

L

F

I

C

binding dihydrolipoic acid: H34 (≠ Q31), L35 (= L32), K63 (= K60)

Sites not aligning to the query:

binding dihydrolipoic acid: 127

P23434 Glycine cleavage system H protein, mitochondrial; Lipoic acid-containing protein from Homo sapiens (Human) (see 4 papers)
43% identity, 95% coverage: 6:121/122 of query aligns to 53:168/173 of P23434

query
sites
P23434

Y

F

T

T

D

E

D

K

H
|
H

E

E

W

W

I

V

E

T

V

T

E

E

D

N

D

G

T

I

A

G

T

T

I

V

G

G

I

I

T

S

K
x
N

H

F

A

A

A
x
Q

E
|
E

Q
x
A

L
|
L

G
|
G

E
x
D

V
|
V

F
x
Y

I
x
C

E
x
S

L
|
L

Q
x
P

P
x
E

E
x
V

G
|
G

E
x
T

T
x
K

F
x
L

V
x
N

K
|
K

G
x
Q

D
|
D

E
|
E

I
x
F

G
|
G

V
x
A

V
x
L

E
|
E

S
|
S

V
|
V

K
|
K

A
|
A

S
|
S

D
x
E

I
x
L

F
x
Y

A
x
S

P
|
P

V
x
L

T
x
S

G
|
G

E
|
E

I
x
V

L
x
T

E
|
E

A
x
I

N
|
N

A
x
E

A
|
A

L
|
L

V
x
A

E
|
E

T
x
N

P
|
P

A
x
G

E
x
L

L
x
V

N
|
N

E
x
K

D
x
S

P
x
C

E
x
Y

G
x
E

N
x
D

S
x
G

W
|
W

L
|
L

Y
x
I

K
|
K

I
x
M

K
x
T

L
|
L

S
|
S

D
x
N

P
|
P

G
x
S

E
|
E

L
|
L

S
x
D

E
|
E

L
|
L

L
x
M

D
x
S

A
x
E

E
|
E

G
x
A

Y
|
Y

A
x
E

A
x
K

L
x
Y

I
|
I

H57 (= H10) to R: in MMDS7; uncertain significance
N73 (≠ K26) to S: in dbSNP:rs8177877
K107 (= K60) modified: N6-lipoyllysine
P115 (= P68) to L: in MMDS7; uncertain significance; reduced lipoylation of DLAT and DLST in homozygous patient cells; no effect on localization to mitochondrion
T148 (≠ K101) to P: in MMDS7; uncertain significance; reduced function in glycine decarboxylation via glycine cleavage system; no effect on localization to mitochondrion

Sites not aligning to the query:

21 S → L: in dbSNP:rs8052579
76:173 natural variant: Missing (in MMDS7; likely pathogenic)

P11183 Glycine cleavage system H protein, mitochondrial; Lipoic acid-containing protein from Gallus gallus (Chicken) (see paper)
43% identity, 95% coverage: 6:121/122 of query aligns to 44:159/164 of P11183

query
sites
P11183

Y

F

T

T

D

D

D

K

H

H

E

E

W

W

I

I

E

S

V

V

E

E

D

N

D

G

T

I

A

G

T

T

I

V

G

G

I

I

T

S

K

N

H

F

A

A

A

Q

E

E

Q

A

L

L

G

G

E

D

V

V

F

Y

I

C

E

S

L

L

Q

P

P

E

E

I

G

G

E

T

T

K

F

L

V

N

K

K

G

D

D

D

E

E

I

F

G

G

V

A

V

L

E

E

S

S

V

V

K
|
K

A

A

S

S

D

E

I

L

F

Y

A

S

P

P

V

L

T

T

G

G

E

E

I

V

L

T

E

D

A

I

N

N

A

A

L

L

V

A

E

D

T

N

P

P

A

G

E

L

L

V

N

N

E

K

D

S

P

C

E

Y

G

Q

N

D

S

G

W

W

L

L

Y

I

K

K

I

M

K

T

L

V

S

E

D

K

P

P

G

A

E

E

L

L

S

D

E

E

L

L

L

M

D

S

A

E

E

D

G

A

Y

Y

A

E

A

K

L

Y

I

I

K98 (= K60) modified: N6-lipoyllysine

Sites not aligning to the query:

1:39 modified: transit peptide, Mitochondrion

P20821 Glycine cleavage system H protein, mitochondrial; Lipoic acid-containing protein from Bos taurus (Bovine) (see 3 papers)
41% identity, 95% coverage: 6:121/122 of query aligns to 53:168/173 of P20821

query
sites
P20821

Y

F

T

T

D

E

D

K

H

H

E

E

W

W

I

V

E

T

V

T

E

E

D

N

D

G

T

V

A

G

T

T

I

V

G

G

I

I

T

S

K

N

H

F

A

A

A

Q

E

E

Q

A

L

L

G

G

E

D

V

V

F

Y

I

C

E

S

L

L

Q

P

P

E

E

V

G

G

E

T

T

K

F

L

V

N

K

K

G

Q

D

E

E

E

I

F

G

G

V

A

V

L

E

E

S

S

V

V

K
|
K

A

A

S

S

D

E

I

L

F

Y

A

S

P

P

V

L

T

S

G

G

E

E

I

V

L

T

E

E

A

I

N

N

A

K

A

A

L

L

V

A

E

E

T

N

P

P

A

G

E

L

L

V

N

N

E

K

D

S

P

C

E

Y

G

E

N

D

S

G

W

W

L

L

Y

I

K

K

I

M

K

T

L

F

S

S

D

N

P

P

G

S

E

E

L

L

S

D

E

E

L

L

L

M

D

S

A

E

E

E

G

A

Y

Y

A

E

A

K

L

Y

I

I

K107 (= K60) modified: N6-lipoyllysine

Sites not aligning to the query:

1:48 modified: transit peptide, Mitochondrion

A0A0H3JT43 Glycine cleavage system H-like protein; GcvH-L from Staphylococcus aureus (strain Mu50 / ATCC 700699) (see paper)
33% identity, 75% coverage: 9:100/122 of query aligns to 6:96/110 of A0A0H3JT43

query
sites
A0A0H3JT43

D

N

H

Y

E

L

W

W

I

V

E

E

V

K

E

V

D

G

D

D

T

L

A

Y

T

V

I

F

G

S

I

M

T

T

K

P

H

E

A

L

A

Q

E

D

Q

D

L

I

G

G

E

T

V

V

V

G

F

Y

I

V

E

E

L

F

Q

V

P

S

E

P

G

D

E

E

T

V

F

K

V

V

K

-

G

D

D

D

E

E

I

I

G

V

V

S

V

I

E
|
E

S

A

V

S

K
|
K

A

T

A

V

S

I

D

D

I

V

F

Q

A

T

P

P

V

L

T

S

G

G

E

T

I

I

L

I

E

E

A

R

N

N

A

T

A

K

L

A

V

E

E

E

T

E

P

P

A

T

E

I

L

L

N

N

-

S

E

E

D

K

P

P

E

E

G

E

N

N

S

-

W

W

L

L

Y

F

K

K

I

L

E53 (= E57) mutation to Q: Decrease in lipoylation.
K56 (= K60) modified: N6-lipoyllysine; mutation to R: Loss of lipoylation.

Query Sequence

>3609295 FitnessBrowser__Dino:3609295
MPTTYYTDDHEWIEVEDDTATIGITKHAAEQLGEVVFIELQPEGETFVKGDEIGVVESVK
AASDIFAPVTGEILEANAALVETPAELNEDPEGNSWLYKIKLSDPGELSELLDAEGYAAL
IG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory