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Comparing 3609474 Dshi_2858 purine nucleoside phosphorylase (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4daeA Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with 6-chloroguanosine (see paper)
54% identity, 99% coverage: 1:231/234 of query aligns to 1:230/232 of 4daeA
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S202 (= S203), D203 (= D204), V205 (≠ L206), R216 (= R217)
- binding 6-chloro-9-(beta-D-ribofuranosyl)-9H-purin-2-amine: M64 (= M64), S90 (= S90), C91 (≠ T91), G92 (= G92), F159 (= F159), E178 (= E179), M179 (= M180), E180 (= E181), S202 (= S203)
4darA Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with tubercidin (see paper)
54% identity, 98% coverage: 2:231/234 of query aligns to 1:229/231 of 4darA
- active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (= D204), V204 (≠ L206), R215 (= R217)
- binding '2-(4-amino-pyrrolo[2,3-d]pyrimidin-7-yl)-5-hydroxymethyl-tetrahydro-furan-3,4-diol: S89 (= S90), C90 (≠ T91), G91 (= G92), F158 (= F159), E177 (= E179), M178 (= M180), E179 (= E181), S201 (= S203)
4dabA Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with hypoxanthine (see paper)
54% identity, 98% coverage: 2:231/234 of query aligns to 1:229/231 of 4dabA
- active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (= D204), V204 (≠ L206), R215 (= R217)
- binding hypoxanthine: G91 (= G92), F158 (= F159), V176 (= V178), M178 (= M180)
4da8A Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with 8-bromoguanosine (see paper)
54% identity, 98% coverage: 2:231/234 of query aligns to 1:229/231 of 4da8A
- active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (= D204), V204 (≠ L206), R215 (= R217)
- binding 8-bromoguanosine: S89 (= S90), G91 (= G92), F158 (= F159), V176 (= V178), E177 (= E179), M178 (= M180), E179 (= E181), S201 (= S203)
4da7A Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with aciclovir (see paper)
54% identity, 98% coverage: 2:231/234 of query aligns to 1:229/231 of 4da7A
- active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (= D204), V204 (≠ L206), R215 (= R217)
- binding 9-hyroxyethoxymethylguanine: C90 (≠ T91), G91 (= G92), F158 (= F159), V176 (= V178), M178 (= M180), E179 (= E181)
- binding phosphate ion: G19 (= G20), R86 (= R87), S89 (= S90)
4da6A Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with ganciclovir (see paper)
54% identity, 98% coverage: 2:231/234 of query aligns to 1:229/231 of 4da6A
- active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (= D204), V204 (≠ L206), R215 (= R217)
- binding 9-(1,3-dihydroxy-propoxymethane)guanine: S89 (= S90), C90 (≠ T91), G91 (= G92), F158 (= F159), V176 (= V178), M178 (= M180), E179 (= E181), S201 (= S203)
4d9hA Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with adenosine (see paper)
54% identity, 98% coverage: 2:231/234 of query aligns to 1:229/231 of 4d9hA
- active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (= D204), V204 (≠ L206), R215 (= R217)
- binding adenosine: M63 (= M64), S89 (= S90), C90 (≠ T91), G91 (= G92), F158 (= F159), V176 (= V178), M178 (= M180), E179 (= E181), S201 (= S203)
4d8vA Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis at ph 4.2 (see paper)
54% identity, 98% coverage: 2:231/234 of query aligns to 1:229/231 of 4d8vA
- active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (= D204), V204 (≠ L206), R215 (= R217)
- binding adenine: G91 (= G92), F158 (= F159), V176 (= V178), E177 (= E179), M178 (= M180)
4danA Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with 2-fluoroadenosine (see paper)
54% identity, 98% coverage: 2:231/234 of query aligns to 1:229/229 of 4danA
- active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (= D204), V204 (≠ L206), R215 (= R217)
- binding 2-(6-amino-2-fluoro-purin-9-yl)-5-hydroxymethyl-tetrahydro-furan-3,4-diol: H3 (= H4), R42 (= R43), S89 (= S90), C90 (≠ T91), G91 (= G92), F158 (= F159), V176 (= V178), E177 (= E179), M178 (= M180), E179 (= E181), S201 (= S203)
4da0A Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with 2'-deoxyguanosine (see paper)
54% identity, 98% coverage: 2:231/234 of query aligns to 1:229/230 of 4da0A
- active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (= D204), V204 (≠ L206), R215 (= R217)
- binding 2'-deoxy-guanosine: S89 (= S90), C90 (≠ T91), G91 (= G92), F158 (= F159), V176 (= V178), E177 (= E179), M178 (= M180), E179 (= E181), S201 (= S203)
3uawA Crystal structure of adenosine phosphorylase from bacillus cereus complexed with adenosine (see paper)
54% identity, 99% coverage: 2:232/234 of query aligns to 1:231/233 of 3uawA
- active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), T89 (≠ S90), S202 (= S203), D203 (= D204), I205 (≠ L206), R216 (= R217)
- binding adenosine: M63 (= M64), T89 (≠ S90), C90 (≠ T91), G91 (= G92), F158 (= F159), E178 (= E179), M179 (= M180), E180 (= E181), D203 (= D204)
- binding sulfate ion: G19 (= G20), R86 (= R87), G88 (= G89), T89 (≠ S90)
3uaxA Crystal structure of adenosine phosphorylase from bacillus cereus complexed with inosine (see paper)
53% identity, 99% coverage: 2:232/234 of query aligns to 1:225/226 of 3uaxA
- active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), T89 (≠ S90), S202 (= S203), D203 (= D204), I205 (≠ L206), R210 (= R217)
- binding inosine: M63 (= M64), T89 (≠ S90), C90 (≠ T91), G91 (= G92), F158 (= F159), E178 (= E179), M179 (= M180), E180 (= E181)
- binding sulfate ion: G19 (= G20), R86 (= R87), G88 (= G89), T89 (≠ S90)
3occF Crystal structure of pnp with dadmeimmh from yersinia pseudotuberculosis
50% identity, 98% coverage: 2:231/234 of query aligns to 2:231/238 of 3occF
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ L206), R217 (= R217)
- binding 7-[[(3R,4R)-3-(hydroxymethyl)-4-oxidanyl-pyrrolidin-1-ium-1-yl]methyl]-3,5-dihydropyrrolo[3,2-d]pyrimidin-4-one: H4 (= H4), R43 (= R43), M64 (= M64), S90 (= S90), C91 (≠ T91), F159 (= F159), V178 (= V178), E179 (= E179), M180 (= M180), E181 (= E181), D204 (= D204), I206 (≠ L206)
P0ABP8 Purine nucleoside phosphorylase DeoD-type; PNP; EC 2.4.2.1 from Escherichia coli (strain K12) (see 5 papers)
50% identity, 99% coverage: 2:233/234 of query aligns to 3:234/239 of P0ABP8
- G21 (= G20) binding in other chain
- R25 (= R24) binding in other chain; mutation to A: Severe loss of catalytic activity. 20-fold decrease in affinity for phosphate.
- K27 (= K26) modified: N6-acetyllysine
- R44 (= R43) binding
- RVGS 88:91 (≠ RIGS 87:90) binding in other chain
- D205 (= D204) active site, Proton donor; mutation D->A,N: Severe loss of catalytic activity.
- R218 (= R217) Important for catalytic activity; mutation to A: Severe loss of catalytic activity.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
P0ABP9 Purine nucleoside phosphorylase DeoD-type; PNP; EC 2.4.2.1 from Escherichia coli O157:H7 (see paper)
50% identity, 99% coverage: 2:233/234 of query aligns to 3:234/239 of P0ABP9
- H5 (= H4) binding
- G21 (= G20) binding in other chain
- R25 (= R24) binding in other chain
- R44 (= R43) binding
- RVGS 88:91 (≠ RIGS 87:90) binding in other chain
- EME 180:182 (= EME 179:181) binding in other chain
- SD 204:205 (= SD 203:204) binding in other chain
5iu6A Crystal structure of e.Coli purine nucleoside phosphorylase with 7- deazahypoxanthine (see paper)
50% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 5iu6A
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ L206), R217 (= R217)
- binding 7H-pyrrolo[2,3-d]pyrimidin-4-ol: C91 (≠ T91), G92 (= G92), F159 (= F159), V178 (= V178), E179 (= E179)
5i3cA Crystal structure of e.Coli purine nucleoside phosphorylase with acycloguanosine (see paper)
50% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 5i3cA
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ L206), R217 (= R217)
- binding 9-hyroxyethoxymethylguanine: S90 (= S90), C91 (≠ T91), G92 (= G92), F159 (= F159), V178 (= V178), E179 (= E179), I206 (≠ L206)
- binding sulfate ion: G20 (= G20), R87 (= R87), G89 (= G89), S90 (= S90)
3ut6A Crystal structure of e. Coli pnp complexed with po4 and formycin a (see paper)
50% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 3ut6A
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ L206), R217 (= R217)
- binding (1S)-1-(7-amino-1H-pyrazolo[4,3-d]pyrimidin-3-yl)-1,4-anhydro-D-ribitol: M64 (= M64), S90 (= S90), C91 (≠ T91), G92 (= G92), F159 (= F159), V178 (= V178), E179 (= E179), M180 (= M180), E181 (= E181), D204 (= D204)
- binding phosphate ion: G20 (= G20), R24 (= R24), R87 (= R87), G89 (= G89), S90 (= S90)
1pw7A Crystal structure of e. Coli purine nucleoside phosphorylase complexed with 9-beta-d-arabinofuranosyladenine and sulfate/phosphate (see paper)
50% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1pw7A
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ L206), R217 (= R217)
- binding phosphate ion: G20 (= G20), R87 (= R87), S90 (= S90)
- binding 2-(6-amino-purin-9-yl)-5-hydroxymethyl-tetrahydro-furan-3,4-diol: S90 (= S90), C91 (≠ T91), F159 (= F159), E179 (= E179), E181 (= E181), S203 (= S203)
1pr0A Escherichia coli purine nucleoside phosphorylase complexed with inosine and phosphate/sulfate (see paper)
50% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1pr0A
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ L206), R217 (= R217)
- binding inosine: R87 (= R87), S90 (= S90), C91 (≠ T91), F159 (= F159), V178 (= V178), E179 (= E179), M180 (= M180), E181 (= E181), D204 (= D204)
- binding phosphate ion: G20 (= G20), R87 (= R87), G89 (= G89), S90 (= S90)
Query Sequence
>3609474 Dshi_2858 purine nucleoside phosphorylase (RefSeq)
MTIHIGAAPGDIAETVLMPGDPLRAKWAAETFLDDPVCVNEVRGMLGFTGTWCGHRVTIH
GSGMGMPSLSIYANELITEYGAQTLIRIGSTGAMQNHVALRDVILAMTCSSISTPSSGIF
CELNFAPCADWGLLKAAEAAAGAKGTKTHVGGIYSSDVFYDERPDLTEQMIRHGVLGVEM
EAAELYTLAARHGRRALAVLTVSDHLLTHDALPSAERQSSFGDMVEIALEAAFA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory