SitesBLAST
Comparing 3609492 Dshi_2876 malate dehydrogenase, NAD-dependent (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3gvhA Crystal structure of lactate/malate dehydrogenase from brucella melitensis
78% identity, 99% coverage: 2:319/320 of query aligns to 1:318/318 of 3gvhA
- active site: R88 (= R89), D148 (= D149), R151 (= R152), H175 (= H176)
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), I13 (= I14), D33 (= D34), I34 (= I35), T77 (= T78), A78 (= A79), I118 (= I119), N120 (= N121), M143 (= M144), L147 (= L148), H175 (= H176)
4rosA Crystal structure of methylobacterium extorquens malate dehydrogenase complexed with oxaloacetate and adenosine-5-diphosphoribose
76% identity, 99% coverage: 2:319/320 of query aligns to 1:318/318 of 4rosA
- active site: R88 (= R89), D148 (= D149), R151 (= R152), H175 (= H176)
- binding adenosine-5-diphosphoribose: G11 (= G12), Q12 (= Q13), I13 (= I14), D33 (= D34), I34 (= I35), T77 (= T78), A78 (= A79), G79 (= G80), V80 (= V81), P81 (= P82), V98 (= V99), T119 (= T120), N120 (= N121)
- binding oxaloacetate ion: R82 (= R83), R88 (= R89), N120 (= N121), L147 (= L148), R151 (= R152), H175 (= H176), G218 (= G219)
5ujkA Malate dehydrogenase from methylobacterium extorquens, complexed with NAD (see paper)
77% identity, 98% coverage: 2:316/320 of query aligns to 1:315/315 of 5ujkA
- active site: R88 (= R89), D148 (= D149), R151 (= R152), H175 (= H176)
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), Q12 (= Q13), I13 (= I14), D33 (= D34), I34 (= I35), T77 (= T78), A78 (= A79), G79 (= G80), V98 (= V99), I118 (= I119), N120 (= N121), M143 (= M144), L147 (= L148), H175 (= H176)
3gviA Crystal structure of lactate/malate dehydrogenase from brucella melitensis in complex with adp
76% identity, 99% coverage: 2:319/320 of query aligns to 1:313/314 of 3gviA
4plyA Crystal structure of ancestral apicomplexan malate dehydrogenase with malate. (see paper)
62% identity, 97% coverage: 3:313/320 of query aligns to 2:317/318 of 4plyA
- active site: D153 (= D149), R156 (= R152), H180 (= H176)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G12), N12 (≠ Q13), I13 (= I14), D33 (= D34), I34 (= I35), Y65 (= Y66), T77 (= T78), A78 (= A79), G79 (= G80), I80 (≠ V81), P81 (= P82), I103 (≠ V99), V123 (≠ I119), N125 (= N121), M148 (= M144), L152 (= L148), H180 (= H176), P239 (= P235)
- binding pyruvic acid: W91 (≠ M87), R93 (= R89), N125 (= N121), R156 (= R152), H180 (= H176), G223 (= G219)
4pltB Crystal structure of ancestral apicomplexan malate dehydrogenase with oxamate. (see paper)
62% identity, 97% coverage: 3:313/320 of query aligns to 3:318/319 of 4pltB
- active site: R94 (= R89), D154 (= D149), R157 (= R152), H181 (= H176)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G12 (= G12), N13 (≠ Q13), I14 (= I14), F33 (= F33), D34 (= D34), I35 (= I35), T78 (= T78), A79 (= A79), G80 (= G80), I81 (≠ V81), P82 (= P82), I104 (≠ V99), V124 (≠ I119), N126 (= N121), M149 (= M144), H181 (= H176), P240 (= P235)
4plfB Crystal structure of ancestral apicomplexan lactate dehydrogenase with lactate. (see paper)
57% identity, 98% coverage: 3:315/320 of query aligns to 4:321/330 of 4plfB
- active site: R95 (= R89), D155 (= D149), R158 (= R152), H182 (= H176)
- binding nicotinamide-adenine-dinucleotide: G13 (= G12), M14 (≠ Q13), I15 (= I14), F34 (= F33), D35 (= D34), V36 (≠ I35), T79 (= T78), A80 (= A79), G81 (= G80), L82 (≠ V81), T83 (≠ P82), I105 (≠ V99), I125 (= I119), N127 (= N121), M150 (= M144), H182 (= H176), P241 (= P235)
- binding pyruvic acid: W93 (≠ M87), R95 (= R89), N127 (= N121), R158 (= R152), H182 (= H176)
4plcA Crystal structure of ancestral apicomplexan lactate dehydrogenase with malate. (see paper)
57% identity, 98% coverage: 3:315/320 of query aligns to 4:321/324 of 4plcA
- active site: R95 (= R89), D155 (= D149), R158 (= R152), H182 (= H176)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G13 (= G12), M14 (≠ Q13), I15 (= I14), D35 (= D34), V36 (≠ I35), T79 (= T78), A80 (= A79), G81 (= G80), L82 (≠ V81), T83 (≠ P82), I125 (= I119), N127 (= N121), M150 (= M144), H182 (= H176), P241 (= P235)
- binding pyruvic acid: W93 (≠ M87), R95 (= R89), N127 (= N121), R158 (= R152), H182 (= H176), G225 (= G219)
6vdjA Crystal structure of ancestral apicomplexan lactate dehydrogenase with sulfate and nadh4.
59% identity, 98% coverage: 3:316/320 of query aligns to 2:308/308 of 6vdjA
- binding 1,4,5,6-tetrahydronicotinamide adenine dinucleotide: G11 (= G12), M12 (≠ Q13), I13 (= I14), D33 (= D34), V34 (≠ I35), A78 (= A79), I91 (≠ V99), I111 (= I119), T112 (= T120), N113 (= N121), M136 (= M144), H168 (= H176)
P49814 Malate dehydrogenase; Vegetative protein 69; VEG69; EC 1.1.1.37 from Bacillus subtilis (strain 168) (see 2 papers)
54% identity, 97% coverage: 2:312/320 of query aligns to 4:311/312 of P49814
- S149 (≠ A145) modified: Phosphoserine
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
7byaA Malate dehydrogenase from geobacillus stearothermophilus (gs-mdh) complexed with oxaloacetic acid (oaa) and adenosine 5'- diphosphoribose (apr) (see paper)
55% identity, 97% coverage: 1:310/320 of query aligns to 2:308/311 of 7byaA
7by9A Malate dehydrogenase from geobacillus stearothermophilus (gs-mdh) complexed with oxaloacetic acid (oaa) and nicotinamide adenine dinucleotide (NAD) (see paper)
55% identity, 97% coverage: 1:310/320 of query aligns to 2:308/311 of 7by9A
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), G13 (= G12), F14 (≠ Q13), T15 (≠ I14), D35 (= D34), I36 (= I35), T81 (= T78), A82 (= A79), I102 (≠ V99), Q105 (≠ S102), V106 (= V103), L122 (≠ I119), N124 (= N121), V126 (≠ L123)
- binding oxaloacetate ion: L151 (= L148), R155 (= R152), H179 (= H176), G217 (= G219)
7f8dA Malate dehydrogenase from geobacillus stearothermophilus (gs-mdh) g218y mutant (see paper)
55% identity, 97% coverage: 1:310/320 of query aligns to 4:310/313 of 7f8dA
- binding nicotinamide-adenine-dinucleotide: G15 (= G12), F16 (≠ Q13), T17 (≠ I14), D37 (= D34), I38 (= I35), L41 (vs. gap), Y71 (= Y66), T83 (= T78), A84 (= A79), G85 (= G80), L124 (≠ I119), T125 (= T120), N126 (= N121), Q149 (≠ M144), L153 (= L148), P235 (= P235)
1uxgA Large improvement in the thermal stability of a tetrameric malate dehydrogenase by single point mutations at the dimer-dimer interface. (see paper)
52% identity, 98% coverage: 3:315/320 of query aligns to 1:308/308 of 1uxgA
- active site: R87 (= R89), D147 (= D149), R150 (= R152), H174 (= H176)
- binding fumaric acid: R81 (= R83), R87 (= R89), L146 (= L148), R150 (= R152), H174 (= H176), G212 (= G219), S223 (= S230)
- binding nicotinamide-adenine-dinucleotide: G10 (= G12), F11 (≠ Q13), V12 (≠ I14), D32 (= D34), T76 (= T78), S77 (≠ A79), G78 (= G80), A79 (≠ V81), P80 (= P82), C101 (≠ V103), V117 (≠ I119), N119 (= N121), Q142 (≠ M144), H174 (= H176), P228 (= P235)
P80040 Malate dehydrogenase; EC 1.1.1.37 from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) (see 2 papers)
51% identity, 98% coverage: 3:315/320 of query aligns to 2:309/309 of P80040
1sowA T. Gondii bradyzoite-specific ldh (ldh2) in complex with NAD and oxalate
51% identity, 95% coverage: 3:305/320 of query aligns to 5:312/322 of 1sowA
- active site: R96 (= R89), D156 (= D149), R159 (= R152), H183 (= H176)
- binding nicotinamide-adenine-dinucleotide: G14 (= G12), M15 (≠ Q13), I16 (= I14), D36 (= D34), V37 (≠ I35), T80 (= T78), A81 (= A79), G82 (= G80), L83 (≠ V81), T84 (≠ P82), I106 (≠ V99), E109 (≠ S102), V126 (≠ I119), T127 (= T120), N128 (= N121), M151 (= M144), L155 (= L148), H183 (= H176), P242 (= P235)
- binding oxalate ion: W94 (≠ M87), R96 (= R89), R159 (= R152), H183 (= H176), G226 (= G219)
3czmB T. Gondii bradyzoite-specific ldh (ldh2) in complex with NAD and oxq
51% identity, 95% coverage: 3:305/320 of query aligns to 6:313/323 of 3czmB
- active site: R97 (= R89), D157 (= D149), R160 (= R152), H184 (= H176)
- binding nicotinamide-adenine-dinucleotide: G15 (= G12), M16 (≠ Q13), I17 (= I14), D37 (= D34), V38 (≠ I35), T81 (= T78), A82 (= A79), G83 (= G80), L84 (≠ V81), T85 (≠ P82), I107 (≠ V99), V127 (≠ I119), N129 (= N121), M152 (= M144), H184 (= H176)
- binding 4-hydroxy-1,2,5-oxadiazole-3-carboxylic acid: W95 (≠ M87), R97 (= R89), N129 (= N121), R160 (= R152), H184 (= H176), G227 (= G219), S238 (= S230)
P80039 Malate dehydrogenase; EC 1.1.1.37 from Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum) (see paper)
51% identity, 98% coverage: 5:318/320 of query aligns to 2:308/310 of P80039
1guzA Structural basis for thermophilic protein stability: structures of thermophilic and mesophilic malate dehydrogenases (see paper)
51% identity, 97% coverage: 5:313/320 of query aligns to 2:303/305 of 1guzA
- active site: D147 (= D149), R150 (= R152), H174 (= H176)
- binding nicotinamide-adenine-dinucleotide: G9 (= G12), N10 (≠ Q13), V11 (≠ I14), D32 (= D34), V33 (≠ I35), T76 (= T78), G78 (= G80), L79 (≠ V81), P80 (= P82), I97 (≠ V99), V117 (≠ I119), N119 (= N121), M142 (= M144), L146 (= L148), H174 (= H176), P228 (= P235)
2hjrB Crystal structure of cryptosporidium parvum malate dehydrogenase (see paper)
52% identity, 98% coverage: 3:315/320 of query aligns to 2:314/314 of 2hjrB
- active site: R88 (= R89), D148 (= D149), R151 (= R152), H175 (= H176)
- binding adenosine-5-diphosphoribose: G11 (= G12), Q12 (= Q13), I13 (= I14), D33 (= D34), I34 (= I35), I35 (≠ A36), Y65 (= Y66), T77 (= T78), A78 (= A79), G79 (= G80), V80 (= V81), P81 (= P82), T119 (= T120), N120 (= N121)
Query Sequence
>3609492 Dshi_2876 malate dehydrogenase, NAD-dependent (RefSeq)
MARPKIALIGAGQIGGTLAHLVALKELGDVVLFDIADGTPQGKALDIAESGPVERFDASL
KGTTDYADIAGADVCIVTAGVPRKPGMSRDDLLGINLKVMKSVGEGIAANAPDAFVICIT
NPLDAMVWALQQFSGLPKEKVVGMAGVLDSARFRHFLAEEFNVSMKDVTAFVLGGHGDTM
VPLTRYSTVAGIPLPDLVEMGWTSQEKLDAIVQRTRDGGAEIVGLLKTGSAFYAPAASAV
EMAEAYLKDQKRLLPCAAYCDGEFGLNDMYVGVPTIIGAGGIEKVVDIKLGKDEQAMFDN
SVNAVKGLMEACKGIDDSLV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory