SitesBLAST
Comparing 3609586 FitnessBrowser__Dino:3609586 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
43% identity, 97% coverage: 7:323/328 of query aligns to 4:321/334 of 5aovA
- active site: L100 (= L104), R241 (= R243), D265 (= D267), E270 (≠ G272), H288 (= H290)
- binding glyoxylic acid: M52 (≠ T55), L53 (≠ V56), L53 (≠ V56), Y74 (= Y78), A75 (≠ G79), V76 (≠ A80), G77 (= G81), R241 (= R243), H288 (= H290)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ A80), T104 (= T108), F158 (≠ M158), G159 (= G159), R160 (= R160), I161 (= I161), S180 (≠ N180), R181 (= R181), A211 (≠ N213), V212 (≠ C214), P213 (= P215), T218 (= T220), I239 (≠ T241), A240 (≠ S242), R241 (= R243), H288 (= H290), G290 (= G292)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
43% identity, 97% coverage: 7:323/328 of query aligns to 3:320/332 of 6biiA
- active site: L99 (= L104), R240 (= R243), D264 (= D267), E269 (≠ G272), H287 (= H290)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ A80), T103 (= T108), G156 (= G157), F157 (≠ M158), G158 (= G159), R159 (= R160), I160 (= I161), A179 (≠ N180), R180 (= R181), S181 (≠ R182), K183 (≠ L184), V211 (≠ C214), P212 (= P215), E216 (≠ S219), T217 (= T220), V238 (≠ T241), A239 (≠ S242), R240 (= R243), D264 (= D267), H287 (= H290), G289 (= G292)
2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
41% identity, 97% coverage: 7:323/328 of query aligns to 4:321/333 of 2dbqA
- active site: L100 (= L104), R241 (= R243), D265 (= D267), E270 (≠ G272), H288 (= H290)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ A80), T104 (= T108), L158 (≠ M158), G159 (= G159), R160 (= R160), I161 (= I161), S180 (≠ N180), R181 (= R181), T182 (≠ R182), A211 (≠ N213), V212 (≠ C214), P213 (= P215), T218 (= T220), I239 (≠ T241), A240 (≠ S242), R241 (= R243), D265 (= D267), H288 (= H290), G290 (= G292)
O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
41% identity, 97% coverage: 7:323/328 of query aligns to 4:321/334 of O58320
Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
38% identity, 92% coverage: 2:302/328 of query aligns to 3:305/328 of Q9UBQ7
2gcgA Ternary crystal structure of human glyoxylate reductase/hydroxypyruvate reductase (see paper)
38% identity, 91% coverage: 6:302/328 of query aligns to 3:301/324 of 2gcgA
- active site: L103 (= L104), R241 (= R243), D265 (= D267), E270 (≠ H271), H289 (= H290)
- binding (2r)-2,3-dihydroxypropanoic acid: L55 (≠ V56), S78 (≠ G79), V79 (≠ A80), G80 (= G81), R241 (= R243), H289 (= H290)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V79 (≠ A80), T107 (= T108), G156 (= G157), G158 (= G159), I160 (= I161), G180 (≠ R181), R181 (= R182), R184 (≠ H185), C212 (= C214), S213 (≠ P215), T218 (= T220), I239 (≠ T241), R241 (= R243), D265 (= D267), H289 (= H290), G291 (= G292)
3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
38% identity, 86% coverage: 36:317/328 of query aligns to 31:304/311 of 3bazA
- active site: L98 (= L104), R230 (= R243), A251 (= A264), D254 (= D267), E259 (≠ G272), H277 (= H290)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (≠ A80), G149 (= G157), L150 (≠ M158), G151 (= G159), R152 (= R160), I153 (= I161), S172 (≠ N180), R173 (= R181), S174 (≠ R182), C201 (= C214), P202 (= P215), T207 (= T220), I228 (≠ T241), G229 (≠ S242), R230 (= R243), D254 (= D267), H277 (= H290), G279 (= G292)
Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
38% identity, 86% coverage: 36:317/328 of query aligns to 33:306/313 of Q65CJ7
5j23A Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with 2'-phospho-adp-ribose (see paper)
38% identity, 94% coverage: 10:317/328 of query aligns to 14:303/318 of 5j23A
- active site: L94 (= L104), R228 (= R243), D252 (= D267), E257 (≠ G272), H275 (= H290)
- binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4r,5r)-3,4,5-trihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: V70 (≠ A80), L148 (≠ M158), G149 (= G159), R150 (= R160), I151 (= I161), T170 (≠ N180), R171 (= R181), P200 (= P215), S204 (= S219), T205 (= T220), R228 (= R243)
5v6qB Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and malonate (see paper)
38% identity, 94% coverage: 10:317/328 of query aligns to 16:305/319 of 5v6qB
- active site: L96 (= L104), R230 (= R243), D254 (= D267), E259 (≠ G272), H277 (= H290)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V72 (≠ A80), V100 (≠ T108), F148 (≠ L156), L150 (≠ M158), G151 (= G159), R152 (= R160), I153 (= I161), T172 (≠ N180), R173 (= R181), V201 (≠ C214), P202 (= P215), S206 (= S219), T207 (= T220), V228 (≠ T241), G229 (≠ S242), R230 (= R243), H277 (= H290), A279 (≠ G292)
5v7nA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and 2-keto-d-gluconic acid (see paper)
38% identity, 94% coverage: 10:317/328 of query aligns to 15:304/319 of 5v7nA
- active site: L95 (= L104), R229 (= R243), D253 (= D267), E258 (≠ G272), H276 (= H290)
- binding 2-keto-D-gluconic acid: G70 (= G79), V71 (≠ A80), G72 (= G81), R229 (= R243), H276 (= H290), S279 (= S293), R285 (= R299)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V71 (≠ A80), V99 (≠ T108), L149 (≠ M158), G150 (= G159), R151 (= R160), I152 (= I161), T171 (≠ N180), R172 (= R181), V200 (≠ C214), P201 (= P215), S205 (= S219), T206 (= T220), V227 (≠ T241), G228 (≠ S242), R229 (= R243), H276 (= H290), A278 (≠ G292)
5v7gA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
37% identity, 97% coverage: 1:317/328 of query aligns to 6:303/317 of 5v7gA
- active site: L94 (= L104), R228 (= R243), D252 (= D267), E257 (≠ G272), H275 (= H290)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V70 (≠ A80), V98 (≠ T108), F146 (≠ L156), L148 (≠ M158), G149 (= G159), R150 (= R160), I151 (= I161), T170 (≠ N180), R171 (= R181), V199 (≠ C214), P200 (= P215), S204 (= S219), T205 (= T220), V226 (≠ T241), G227 (≠ S242), R228 (= R243), H275 (= H290), A277 (≠ G292)
- binding oxalate ion: G69 (= G79), V70 (≠ A80), G71 (= G81), R228 (= R243), H275 (= H290)
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
32% identity, 86% coverage: 28:310/328 of query aligns to 3:289/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (= R160), Y160 (≠ H179), D161 (≠ N180), P162 (≠ R181), I164 (= I188), L179 (= L200), T193 (≠ C214), P194 (= P215), S198 (= S219), L202 (= L223)
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
33% identity, 84% coverage: 37:310/328 of query aligns to 30:297/297 of 6rj3A
7dkmA Phgdh covalently linked to oridonin (see paper)
33% identity, 84% coverage: 37:310/328 of query aligns to 32:299/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ A80), A102 (≠ T108), G148 (= G157), R151 (= R160), I152 (= I161), Y170 (≠ H179), D171 (≠ N180), P172 (≠ R181), I173 (≠ R182), H202 (≠ N213), T203 (≠ C214), P204 (= P215), T209 (= T220), C230 (≠ T241), A231 (≠ S242), R232 (= R243), H279 (= H290), G281 (= G292)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: E293 (= E304)
Sites not aligning to the query:
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
33% identity, 84% coverage: 37:310/328 of query aligns to 31:298/303 of 6plgA
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
33% identity, 84% coverage: 37:310/328 of query aligns to 30:297/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (≠ L104), A100 (≠ T108), R149 (= R160), I150 (= I161), Y168 (≠ H179), D169 (≠ N180), P170 (≠ R181), I171 (≠ R182), H200 (≠ N213), T201 (≠ C214), P202 (= P215), T207 (= T220), C228 (≠ T241), A229 (≠ S242), R230 (= R243), H277 (= H290), G279 (= G292)
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
33% identity, 84% coverage: 37:310/328 of query aligns to 31:298/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (= L156), G147 (= G157), L148 (≠ M158), G149 (= G159), R150 (= R160), I151 (= I161), G152 (= G162), D170 (≠ N180), H201 (≠ N213), T202 (≠ C214), P203 (= P215)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
33% identity, 84% coverage: 37:310/328 of query aligns to 31:298/302 of 6rihA
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
33% identity, 84% coverage: 37:310/328 of query aligns to 31:298/301 of 6rj5A
Query Sequence
>3609586 FitnessBrowser__Dino:3609586
MPGERLSVVVTRRLPEAVETRLKELFDVELNESDTPMSRDALQAAMRRADVLVPTVSDHI
DGAMLAGVGDRLKLIANYGAGVDHIDVATARQRGIHVSNTPGVLTDDTADMTLALILAVT
RRIPEGLALMQTGAWTGWSPTALMGGRIAGRRLGILGMGRIGQAVARRAKAFGMQIHYHN
RRRLHKGIEEELEATWWESLDQMVSRMDVISVNCPHTPSTFHLMNARRLKLMKPSAVIVN
TSRGEVIDENALTRMLRAGDIAGAGLDVFEHGHEVNPRLRELPNVVLLPHMGSATEEGRA
EMGEKVIINIKTFDDGHRPPDLVVPSML
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory