SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3609905 FitnessBrowser__Dino:3609905 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

D7A5Q8 Aromatic amino acid exporter YddG from Starkeya novella (strain ATCC 8093 / DSM 506 / JCM 20403 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIMB 10456) (see paper)
30% identity, 49% coverage: 1:141/288 of query aligns to 1:139/287 of D7A5Q8

query
sites
D7A5Q8

M

M

M

S

D

R

L

S

R

S

A

A

L

T

V

L

M

I

G

G

L

F

G

T

F

A

A

I

L

L

M

L

W

W

S

S

S

T

A

-

F

L

T

A

S

L

A

A

R

T

I

S

V

T

A

G

A

A

A

V

P

P

L

F

T

L

L

T

S

A

L

L

R

T

F

F

L

T

I

I

S

G

G

G

L

A

I

V

A

G

V

I

G

A

I

A

A

G

L

L

A

A

L

R

G

G

Q

V

T

G

W

L

R

S

L

V

S

L

R

R

T

Q

Q

P

W

W

K

P

A

-

T

V

F

W

L

V

F

H

G

G

L

I

C

G

Q

G

N

L

A

F

L

G

Y
|
Y

L
x
H

G

F

L

F

N
x
Y

F

F

V

S

A

A

M

L

Q

K

T

L

V

A

Q

P

A

P

S

A

L

E

A

A

A

G

I

L

I

V

A

A

S

Y

T

L

M
x
W

P

P

L

L

V

L

V

I

A

V

L

L

A

F

S

S

W

A

L

F

V

L

L

P

R

G

E

E

R

R

L

L

R

R

P

P

L

A

A

H

M

V

V

A

G

G

L

A

L

L

A

M

G

G

I

L

A

A

G

G

V

T

V

V

L

V

I

L

M

L

G

G

A

A

R

R

Y78 (= Y80) mutation to A: Shows moderate effects on the transport activities of both threonine and methionine.
H79 (≠ L81) mutation to A: Abolishes the transport activities for both threonine and methionine.
Y82 (≠ N84) mutation to A: Enhances methionine transport and slightly reduces threonine transport.
W101 (≠ M103) mutation to A: Exhibits decreased transport activity for threonine, but not for methionine.

Sites not aligning to the query:

163 W→A: Exhibits decreased transport activity for threonine, but not for methionine.

Query Sequence

>3609905 FitnessBrowser__Dino:3609905
MMDLRALVMGLGFALMWSSAFTSARIIVAAAPPLTTLSLRFLISGLIAVGIALALGQTWR
LSRTQWKATFLFGLCQNALYLGLNFVAMQTVQASLAAIIASTMPLVVALASWLVLRERLR
PLAMVGLLAGIAGVVLIMGARLQAGVDLVGVLYCGIGVLALAFATLALRTASSGGNLLMV
VGLQMLVGAVALALVGLPTETLEVTWSWQLVVAFAYTTLVPGLAATFVWFLLVDRIGAVR
AATFHFLNPVFGVAIAAALLGEALGARDVVGVAIVTLGILAVQLSRAR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory