SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3609919 FitnessBrowser__Dino:3609919 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
39% identity, 93% coverage: 7:274/289 of query aligns to 4:276/306 of 4whxA

query
sites
4whxA

D

D

R

R

D

D

G

G

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K

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I

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W

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M

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D

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A

A

active site: F37 (= F40), K160 (= K158), E194 (= E192), L217 (= L215)
binding alanine: Y97 (= Y95), K160 (= K158), A258 (= A256)

2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
40% identity, 89% coverage: 8:265/289 of query aligns to 3:265/305 of 2ej0B

query
sites
2ej0B

R

K

D

A

G

G

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L

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I

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W

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M

D

N

G

G

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A

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E

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A

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F
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F

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G

E

I

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A

A

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Y

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A
x
E

N

N

I

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F

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K

R

D

D

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G

E

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V

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I

active site: F35 (= F40), G37 (= G42), K158 (= K158), E192 (= E192), L215 (= L215)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R59), Y163 (= Y163), E192 (= E192), G195 (= G195), E196 (≠ A196), L215 (= L215), G217 (= G217), I218 (= I218), T219 (= T219), G254 (= G254), T255 (= T255)

2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
39% identity, 89% coverage: 8:265/289 of query aligns to 3:257/297 of 2ej3A

query
sites
2ej3A

R

K

D

A

G

G

T

L

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I

W

W

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M

D

N

G

G

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A

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H

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F
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E

E

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G

E

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A

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Y

N

E

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A

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K

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Y

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A

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A

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I

active site: F35 (= F40), G37 (= G42), K150 (= K158), E184 (= E192), L207 (= L215)
binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G195), G246 (= G254), T247 (= T255), A248 (= A256)
binding pyridoxal-5'-phosphate: R58 (= R59), K150 (= K158), Y155 (= Y163), E184 (= E192), G187 (= G195), L207 (= L215), G209 (= G217), I210 (= I218), T211 (= T219), G246 (= G254), T247 (= T255)

2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
39% identity, 89% coverage: 8:265/289 of query aligns to 3:257/297 of 2eiyA

query
sites
2eiyA

R

K

D

A

G

G

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L

I

I

W

W

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M

D

N

G

G

K

A

L

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R

E

A

E

A

A

N

K

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H

S

I

V

L

L

T

S

H

H

A

A

M

L

H

H

Y

Y

A

G

S

T

S

S

V

V

F
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F

E

E

G
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G

E

I

R

R

A

A

Y

Y

N

E

G

T

K

A

-

K

-

G

-

P

-

A

I

I

F

F

E

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L

R

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R

E

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L

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A

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E

E

I

L

E

E

A

E

A

A

K

I

Y

K

E

E

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M

V

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R

V

R

N

N

G

G

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Y

S

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D

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A

C

Y
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Y

V

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A

A

W

W

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M

G

G

A

A

G

-

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D

A

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L

G

G

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A

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P

-

L

A

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R

N

N

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A

H

E

L

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A

M

I

V

A

A

C

A

W

W

E

E

W

W

G

-

S

-

Y

-

G

-

D

-

A

-

K

V

T

R

R

K

G

G

A

A

K

R

L

L

D

I

I

T

A

S

K

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W

K

A

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R

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F

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P

A

E

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P

P

V

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H

K

A

A

K
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K

A

V

A

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G

G

L

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M

V

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A

T

L

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A

K

K

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A

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A

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A

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A

A

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R

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G

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V

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A

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E
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E

A

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T

S

G
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G

A
x
E

N

N

I

L

F

F

V

V

K

R

D

D

G

G

E

V

V

I

H

Y

T

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D

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P

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F

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L
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L

N

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G

I
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M

I

I

active site: F35 (= F40), G37 (= G42), K150 (= K158), E184 (= E192), L207 (= L215)
binding 4-methyl valeric acid: F35 (= F40), Y94 (= Y95), T247 (= T255), A248 (= A256)
binding pyridoxal-5'-phosphate: R58 (= R59), K150 (= K158), Y155 (= Y163), E184 (= E192), G187 (= G195), E188 (≠ A196), L207 (= L215), G209 (= G217), I210 (= I218), T211 (= T219), G246 (= G254), T247 (= T255)

1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
39% identity, 89% coverage: 8:265/289 of query aligns to 3:257/297 of 1wrvA

query
sites
1wrvA

R

K

D

A

G

G

T

L

I

I

W

W

M

M

D

N

G

G

K

A

L

F

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A

E

A

A

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L

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H

A

A

M

L

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H

Y

Y

A

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S

S

V

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F
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F

E

E

G
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G

E

I

R

R

A

A

Y

Y

N

E

G

T

K

A

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K

-

G

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P

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A

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I

F

F

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R

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H

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E

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F

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E

E

I

L

E

E

A

E

A

A

K

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Y

K

E

E

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V

M

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K

R

V

R

N

N

G

G

L

Y

S

R

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S

A

C

Y

Y

V

I

R

R

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V

L

A

A

W

W

R

M

G

G

A

A

G

-

P

K

D

A

M

L

G

G

V

V

A

N

S

P

-

L

A

P

R

N

N

N

P

P

V

A

H

E

L

V

A

M

I

V

A

A

C

A

W

W

E

E

W

W

G

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S

-

Y

-

G

-

D

-

A

-

K

V

T

R

R

K

G

G

A

A

K

R

L

L

D

I

I

T

A

S

K

S

W

W

K

A

R

R

P

F

S

P

P

A

E

N

T

V

I

M

P

P

V

G

H

K

A

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

T

L

S

A

K

K

H

M

A

E

A

A

E

V

A

A

K

A

G

G

C

A

S

D

D

E

A

A

L

L

F

L

M

L

D

D

Y

E

R

E

G

G

Y

Y

V

V

A

A

E
|
E

A

G

T

S

G
|
G

A

E

N

N

I

L

F

F

V

V

K

R

D

D

G

G

E

V

V

I

H

Y

T

A

P

L

D

E

P

H

D

S

C

V

F

N

L
|
L

N

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

V

V

I

I

G

R

M

I

L

A

R

K

D

D

K

L

Q

G

V

Y

K

E

V

V

H

Q

E

V

R

V

H

R

I

A

M

T

P

R

E

D

E

Q

L

L

E

Y

G

M

F

A

E

D

Q

E

C

V

W

F

L

M

T

T

G

G

T
|
T

A

A

E

E

V

V

T

T

P

P

V

V

G

S

Q

M

I

I

active site: F35 (= F40), G37 (= G42), K150 (= K158), E184 (= E192), L207 (= L215)
binding pyridoxal-5'-phosphate: R58 (= R59), K150 (= K158), Y155 (= Y163), E184 (= E192), G187 (= G195), L207 (= L215), G209 (= G217), I210 (= I218), T211 (= T219), T247 (= T255)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
39% identity, 89% coverage: 8:265/289 of query aligns to 3:254/294 of 2ej2A

query
sites
2ej2A

R

K

D

A

G

G

T

L

I

I

W

W

M

M

D

N

G

G

K

A

L

F

V

V

D

P

W

Q

R

E

A

E

A

A

N

K

V

T

H

S

I

V

L

L

T

S

H

H

A

A

M

L

H

H

Y

Y

A

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

A

A

Y

Y

N

E

G

T

K

A

-

K

-

G

-

P

-

A

I

I

F

F

E

R

S

L

R

K

R

E

H

H

S

V

E

K

R
|
R

L

F

H

Y

F

N

S

S

A

A

G

K

E

V

L

L

D

R

I

M

E

E

I

I

P

P

F

F

T

A

V

P

D

E

E

E

I

L

E

E

A

E

A

A

K

I

Y

K

E

E

V

V

M

V

K

R

V

R

N

N

G

G

L

Y

S

R

D

S

A

C

Y
|
Y

V

I

R

R

A

P

V

L

A

A

W

W

R

M

G

G

A

A

G

-

P

K

D

A

M

L

G

G

V

V

A

N

S

P

-

L

A

P

R

N

N

N

P

P

V

A

H

E

L

V

A

M

I

V

A

A

C

A

W

W

E

E

W

W

G

G

S

-

Y

-

G

-

D

-

A

-

K

-

T

-

R

-

G

-

A

A

K

R

L

L

D

I

I

T

A

S

K

S

W

W

K

A

R

R

P

F

S

P

P

A

E

N

T

V

I

M

P

P

V

G

H

K

A

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

T

L

S

A

K

K

H

M

A

E

A

A

E

V

A

A

K

A

G

G

C

A

S

D

D

E

A

A

L

L

F

L

M

L

D

D

Y

E

R

E

G

G

Y

Y

V

V

A

A

E
|
E

A

G

T

S

G
|
G

A
x
E

N

N

I

L

F

F

V

V

K

R

D

D

G

G

E

V

V

I

H

Y

T

A

P

L

D

E

P

H

D

S

C

V

F

N

L
|
L

N

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

V

V

I

I

G

R

M

I

L

A

R

K

D

D

K

L

Q

G

V

Y

K

E

V

V

H

Q

E

V

R

V

H

R

I

A

M

T

P

R

E

D

E

Q

L

L

E

Y

G

M

F

A

E

D

Q

E

C

V

W

F

L

M

T

T

G

G

T
|
T

A
|
A

A

A

E

E

V

V

T

T

P

P

V

V

G

S

Q

M

I

I

active site: F35 (= F40), G37 (= G42), K147 (= K158), E181 (= E192), L204 (= L215)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R59), Y94 (= Y95), Y152 (= Y163), E181 (= E192), G184 (= G195), E185 (≠ A196), L204 (= L215), G206 (= G217), I207 (= I218), T208 (= T219), T244 (= T255), A245 (= A256)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
35% identity, 95% coverage: 12:285/289 of query aligns to 5:280/290 of 5e25A

query
sites
5e25A

I

V

W

Y

M

M

D

N

G

G

K

E

L

F

V

V

D

P

W

E

R

S

A

Q

A

A

N

K

V

V

H

S

I

V

L

F

T

D

H

H

A

G

M

F

H

L

Y

Y

A

G

S

D

S

G

V

V

F
|
F

E

E

G
|
G

E

I

R

R

A

A

Y

Y

N

N

G

G

K

K

I

V

F

F

E

K

S

L

R

Y

R

E

H

H

S

I

E

D

R
|
R

L

L

H

Y

F

D

S

C

A

A

G

R

E

V

L

I

D

D

I

L

E

K

I

I

P

P

F

L

T

S

V

K

D

E

E

E

I

F

E

A

A

E

A

A

K

I

Y

L

E

E

V

T

M

L

K

R

V

R

N

N

G

N

L

L

S

R

D

D

A

A

Y
|
Y

V

I

R

R

A

P

V

I

A

V

W

T

R

R

G

G

A

A

G

G

P

-

D

D

M

L

G

G

V

L

A

D

S

P

A

R

R

K

N

C

P

P

V

S

-

P

H

N

L

V

A

I

I

I

A

I

C

T

W

K

E

P

W

W

G

G

S

K

Y

L

Y

Y

G

G

D

D

A

L

K

Y

T

E

R

K

G

G

A

L

K

K

L

A

D

I

I

T

A

V

K

A

W

I

K

R

R

R

P

N

S

A

P

I

E

D

T

S

I

L

P

P

V

P

H

N

A

I

K
|
K

A

S

A

L

G

N

L

-

Y
|
Y

M

L

I

N

C

N

T

I

T

L

S

A

K

K

H

I

A

E

A

A

E

N

A

A

K

K

G

G

C

G

S

D

D

E

A

A

L

I

F

F

M

L

D

D

Y

H

R

N

G

G

Y

Y

V

I

A

S

E
|
E

A

G

T

S

G
|
G

A
x
D

N

N

I

I

F

F

F

I

V

V

K

K

D

N

G

G

E

T

V

I

H

T

T

T

P

P

D

P

P

T

D

L

C

N

F

N

L
|
L

N

K

G
|
G

I
|
I

T
|
T

R

R

Q

Q

T

V

V

V

I

I

G

E

M

L

L

I

R

N

D

E

K

L

Q

E

V

I

K

P

V

F

H

R

E

E

R

A

H

N

I

I

M

G

P

L

E

F

E

D

L

L

E

Y

G

S

F

A

E

D

Q

E

C

I

W

F

L

V

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

A

P

P

V

V

G

T

Q

Y

I

I

G

D

-

G

-

R

-

T

T

V

Y

G

N

N

F

G

E

K

V

P

G

G

A

K

L

V

T

T

R

K

D

M

I

L

A

M

E

E

S

K

Y

F

E

R

Q

E

active site: F33 (= F40), G35 (= G42), K151 (= K158), E184 (= E192), L207 (= L215)
binding 2-oxoglutaric acid: Y88 (= Y95), K151 (= K158), T247 (= T255), A248 (= A256)
binding pyridoxal-5'-phosphate: R52 (= R59), K151 (= K158), Y155 (= Y163), E184 (= E192), G187 (= G195), D188 (≠ A196), L207 (= L215), G209 (= G217), I210 (= I218), T211 (= T219), G246 (= G254), T247 (= T255)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
36% identity, 96% coverage: 12:287/289 of query aligns to 9:286/301 of 6thqB

query
sites
6thqB

I

V

W

W

M

L

D

D

G

G

K

R

L

L

V

V

D

D

W

E

R

E

A

E

A

A

N

K

V

V

H

T

I

V

L

L

T

S

H

P

A

S

M

L

H

N

Y

Y

A

G

S

F

S

G

V

V

F
|
F

E

E

G

G

E

I

R

R

A

A

Y

Y

-

W

N

N

G

G

K

E

-

N

-

L

-

Y

I

V

F

F

E

R

S

L

R

R

R

D

H

H

S

M

E

E

R
|
R

L

L

H

L

F

R

S

S

A

A

G

K

E

I

L

I

D

G

I

L

E

D

I

V

P

P

F

Y

T

T

V

A

D

E

E

E

I

L

E

S

A

K

A

A

K

V

Y

V

E

E

V

T

M

V

K

R

V

A

N

N

G

G

L

F

S

K

-

E

D

D

A

L

Y
|
Y

V

I

R

R

A

P

V

V

A

A

W

Y

R

I

G

-

A

S

G

K

P

P

D

Q

M

I

G

S

V

L

A

D

S

V

A

R

R

G

N

L

P

Q

V

A

H

S

L

V

A

A

I

I

A

A

C

A

W

I

E

P

W

F

G

G

S

K

Y

Y

Y

L

G

-

D

-

A

-

K

K

T

V

R

E

G

G

A

V

K

R

L

A

D

A

I

V

A

V

K

S

W

W

K

R

R

R

P

V

S

H

P

T

E

S

T

M

I

M

P

P

V

V

H

M

A

A

K
|
K

A

A

A

T

G

G

L

I

Y
|
Y

M

L

I

N

C

S

T

I

T

M

S

A

K

A

H

V

A

E

A

A

E

R

A

A

K

R

G

G

C

Y

S

D

D

E

A

A

L

I

F

M

M

L

D

N

Y

A

R

E

G

G

Y

K

V

V

A

V

E
|
E

A

G

T

S

G
|
G

A
x
E

N
|
N

I

I

F

F

F

I

V

V

K

R

D

R

G

G

E

V

V

L

H

M

T

T

P

P

D

P

-

L

P

E

D

D

C

G

F

I

L
|
L

N

E

G
|
G

I
|
I

T
|
T

R

R

Q

E

T

T

V

V

I

I

G

S

M

I

L

A

R

G

D

D

K

L

Q

G

V

I

K

P

V

L

H

L

E

E

R

K

H

S

I

I

M

T

P

R

E

E

L

L

E

Y

G

A

F

A

E

D

Q

E

C

A

W

F

L

F

T

V

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

V

I

G

I

Q

E

I

I

G

D

T

G

Y

R

N

V

F

L

E

Q

V

R

G

G

A

P

L

I

T

T

R

Q

D

K

I

I

A

A

E

E

S

T

Y

Y

E

R

Q

R

L

I

V

V

active site: F37 (= F40), K156 (= K158), E190 (= E192), L214 (= L215)
binding pyridoxal-5'-phosphate: R60 (= R59), K156 (= K158), Y161 (= Y163), E190 (= E192), N195 (= N197), L214 (= L215), G216 (= G217), I217 (= I218), T218 (= T219), T254 (= T255)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R59), Y97 (= Y95), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), E194 (≠ A196), N195 (= N197), G216 (= G217), I217 (= I218), T218 (= T219), G253 (= G254), T254 (= T255), A255 (= A256)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
35% identity, 96% coverage: 12:289/289 of query aligns to 4:283/290 of 5mr0D

query
sites
5mr0D

I

V

W

Y

M

M

D

D

G

G

K

E

L

F

V

V

D

P

W

E

R

N

A

E

A

A

N

K

V

V

H

S

I

I

L

F

T

D

H

H

A

G

M

F

H

L

Y

Y

A

G

S

D

S

G

V

V

F
|
F

E

E

G
|
G

E

I

R

R

A

A

Y

Y

N

N

G

G

K

R

I

V

F

F

E

R

S

L

R

K

R

E

H

H

S

I

E

D

R
|
R

L

L

H

Y

F

D

S

S

A

A

G

K

E

A

L

I

D

D

I

L

E

E

I

I

P

P

F

I

T

T

V

K

D

E

E

E

I

F

E

M

A

E

A

I

K

I

Y

L

E

E

V

T

M

L

K

R

V

K

N

N

G

N

L

L

S

R

D

D

A

A

Y

Y

V

I

R

R

A

P

V

I

A

V

W

T

R

R

G

G

A

I

G

G

P

-

D

D

M

L

G
|
G

V
x
L

-

D

-

P

A

R

S

K

A

C

R

Q

N

N

P

P

V

-

H

S

L

I

A

I

I

V

A

I

C

T

W

K

E

P

W

W

G

G

S

K

Y

L

Y

Y

G

G

D

D

A

L

K

Y

T

E

R

K

G

G

A

L

K

T

L

A

D

I

I

T

A

V

K

A

W

V

K

R

R

R

P

N

S

S

P

F

E

D

T

A

I

L

P

P

V

P

H

N

A

I

K
|
K

A

S

A

L

G

N

L

-

Y
|
Y

M

L

I

N

C

N

T

I

T

L

S

A

K

K

H

I

A

E

A

A

E

N

A

A

K

K

G

G

C

G

S

D

D

E

A

A

L

I

F

F

M

L

D

D

Y

R

R

N

G

G

Y

Y

V

V

A

S

E
|
E

A

G

T

S

G
|
G

A
x
D

N

N

I

I

F

F

F

V

V

V

K

K

D

N

G

G

E

A

V

I

H

T

T

T

P

P

D

P

P

T

D

I

C

N

F

N

L
|
L

N

R

G

G

I
|
I

T
|
T

R

R

Q

E

T

A

V

V

I

I

G

E

M

I

L

I

R

N

D

R

K

L

Q

G

V

I

K

P

V

F

H

K

E

E

R

T

H

N

I

I

M

G

P

L

E

Y

E

D

L

L

E

Y

G

T

F

A

E

D

Q

E

C

V

W

F

L

V

T

T

G
|
G

T
|
T

A

A

E

E

V

I

T

A

P

P

V

I

G

V

Q

V

I

I

G

D

T

G

Y

R

N

K

F

I

-

G

-

D

-

G

E

K

V

P

G

G

A

E

L

I

T

T

R

R

D

K

I

L

A

M

E

E

S

E

Y

F

E

S

Q

K

L

L

V

T

R

E

A

S

active site: F32 (= F40), G34 (= G42), K150 (= K158), E183 (= E192), L206 (= L215)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R59), G100 (= G109), L101 (≠ V110), K150 (= K158), Y154 (= Y163), E183 (= E192), G186 (= G195), D187 (≠ A196), L206 (= L215), I209 (= I218), T210 (= T219), G245 (= G254), T246 (= T255)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
34% identity, 94% coverage: 12:283/289 of query aligns to 5:285/304 of 1iyeA

query
sites
1iyeA

I

I

W

W

M

F

D

N

G

G

K

E

L

M

V

V

D

R

W

W

R

E

A

D

A

A

N

K

V

V

H

H

I

V

L

M

T

S

H

H

A

A

M

L

H

H

Y

Y

A

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

A

C

Y

Y

N

D

G

S

K

H

-

K

-

G

-

P

-

V

I

V

F

F

E

R

S

H

R

R

R

E

H

H

S

M

E

Q

R
|
R

L

L

H

H

F

D

S

S

A

A

G

K

E

I

L

Y

D

R

I

F

E

P

I

V

P

S

F

Q

T

S

V

I

D

D

E

E

I

L

E

M

A

E

A

A

K

C

Y

R

E

D

V

V

M

I

K

R

V

K

N

N

G

N

L

L

S

T

D

S

A

A

Y
|
Y

V

I

R

R

A

P

V

L

A

I

W

F

R

V

G

G

A

-

G

-

P

-

D

D

M

V

G

G

V

M

A

G

S

V

A

-

R

-

N

N

P

P

-

P

-

A

-

G

-

Y

-

S

V

T

H

D

L

V

A

I

I

I

A

A

C

A

W

F

E

P

W

W

G

G

S

A

Y
|
Y

Y

L

G

G

-

A

D

E

A

A

K

L

T

E

R

Q

G

G

A

I

K

D

L

A

D

M

I

V

A

S

K

S

W

W

K

N

R

R

P

A

S

A

P

P

E

N

T

T

I

I

P

P

V

T

H

A

A

A

K
|
K

A
|
A

A

G

G

G

L

N

Y
|
Y

M

L

I

S

C

S

T

L

S

V

K

G

H

S

A

E

A

A

E

R

A

R

K

H

G

G

C

Y

S

Q

D

E

A

G

L

I

F

A

M

L

D

D

Y

V

R

N

G

G

Y

Y

V

I

A

S

E
|
E

A

G

T

A

G
|
G

A
x
E

N
|
N

I

L

F

F

F

E

V

V

K

K

D

D

G

G

E

V

V

L

H

F

T

T

P

P

D

P

-

F

P

T

D

S

C

S

F

A

L
|
L

N

P

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

A

V

I

I

I

G

K

M

L

L

A

R

K

D

E

K

L

Q

G

V

I

K

E

V

V

H

R

E

E

R

Q

H

V

I

L

M

S

P

R

E

E

E

S

L

L

E

Y

G

L

F

A

E

D

Q

E

C

V

W

F

L

M

T

S

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

V

V

G

R

Q

S

I

V

G

D

T

G

Y

I

N

Q

F

V

-

G

-

E

-

G

E

R

V

C

G

G

A

P

L

V

T

T

R

K

D

R

I

I

A

Q

E

Q

S

A

Y

F

active site: F33 (= F40), G35 (= G42), K156 (= K158), A157 (= A159), E190 (= E192), L214 (= L215)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R59), Y92 (= Y95), Y126 (= Y129), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), E194 (≠ A196), N195 (= N197), L214 (= L215), G216 (= G217), I217 (= I218), T218 (= T219), G253 (= G254), T254 (= T255), A255 (= A256)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
34% identity, 94% coverage: 12:283/289 of query aligns to 5:285/304 of 1iydA

query
sites
1iydA

I

I

W

W

M

F

D

N

G

G

K

E

L

M

V

V

D

R

W

W

R

E

A

D

A

A

N

K

V

V

H

H

I

V

L

M

T

S

H

H

A

A

M

L

H

H

Y

Y

A

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

A

C

Y

Y

N

D

G

S

K

H

-

K

-

G

-

P

-

V

I

V

F

F

E

R

S

H

R

R

R

E

H

H

S

M

E

Q

R
|
R

L

L

H

H

F

D

S

S

A

A

G

K

E

I

L

Y

D

R

I

F

E

P

I

V

P

S

F

Q

T

S

V

I

D

D

E

E

I

L

E

M

A

E

A

A

K

C

Y

R

E

D

V

V

M

I

K

R

V

K

N

N

G

N

L

L

S

T

D

S

A

A

Y
|
Y

V

I

R

R

A

P

V

L

A

I

W

F

R

V

G

G

A

-

G

-

P

-

D

D

M

V

G

G

V

M

A

G

S

V

A

-

R

-

N

N

P

P

-

P

-

A

-

G

-

Y

-

S

V

T

H

D

L

V

A

I

I

I

A

A

C

A

W

F

E

P

W

W

G

G

S

A

Y
|
Y

Y

L

G

G

-

A

D

E

A

A

K

L

T

E

R

Q

G

G

A

I

K

D

L

A

D

M

I

V

A

S

K

S

W

W

K

N

R

R

P

A

S

A

P

P

E

N

T

T

I

I

P

P

V

T

H

A

A

A

K
|
K

A
|
A

A

G

G

G

L

N

Y
|
Y

M

L

I

S

C

S

T

L

S

V

K

G

H

S

A

E

A

A

E

R

A

R

K

H

G

G

C

Y

S

Q

D

E

A

G

L

I

F

A

M

L

D

D

Y

V

R

N

G

G

Y

Y

V

I

A

S

E
|
E

A

G

T

A

G
|
G

A
x
E

N

N

I

L

F

F

F

E

V

V

K

K

D

D

G

G

E

V

V

L

H

F

T

T

P

P

D

P

-

F

P

T

D

S

C

S

F

A

L
|
L

N

P

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

A

V

I

I

I

G

K

M

L

L

A

R

K

D

E

K

L

Q

G

V

I

K

E

V

V

H

R

E

E

R

Q

H

V

I

L

M

S

P

R

E

E

E

S

L

L

E

Y

G

L

F

A

E

D

Q

E

C

V

W

F

L

M

T

S

G

G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

V

V

G

R

Q

S

I

V

G

D

T

G

Y

I

N

Q

F

V

-

G

-

E

-

G

E

R

V

C

G

G

A

P

L

V

T

T

R

K

D

R

I

I

A

Q

E

Q

S

A

Y

F

active site: F33 (= F40), G35 (= G42), K156 (= K158), A157 (= A159), E190 (= E192), L214 (= L215)
binding glutaric acid: Y92 (= Y95), Y126 (= Y129), A255 (= A256)
binding pyridoxal-5'-phosphate: R56 (= R59), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), E194 (≠ A196), L214 (= L215), G216 (= G217), I217 (= I218), T218 (= T219), T254 (= T255)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
34% identity, 94% coverage: 12:283/289 of query aligns to 5:285/304 of 1i1mA

query
sites
1i1mA

I

I

W

W

M

F

D

N

G

G

K

E

L

M

V

V

D

R

W

W

R

E

A

D

A

A

N

K

V

V

H

H

I

V

L

M

T

S

H

H

A

A

M

L

H

H

Y

Y

A

G

S

T

S

S

V

V

F

F

E

E

G

G

E

I

R

R

A

C

Y

Y

N

D

G

S

K

H

-

K

-

G

-

P

-

V

I

V

F

F

E

R

S

H

R

R

R

E

H

H

S

M

E

Q

R
|
R

L

L

H

H

F

D

S

S

A

A

G

K

E

I

L

Y

D

R

I

F

E

P

I

V

P

S

F

Q

T

S

V

I

D

D

E

E

I

L

E

M

A

E

A

A

K

C

Y

R

E

D

V

V

M

I

K

R

V

K

N

N

G

N

L

L

S

T

D

S

A

A

Y
|
Y

V

I

R

R

A

P

V

L

A

I

W

F

R

V

G

G

A

-

G

-

P

-

D

D

M

V

G

G

V

M

A

G

S

V

A

-

R

-

N

N

P

P

-

P

-

A

-

G

-

Y

-

S

V

T

H

D

L

V

A

I

I

I

A

A

C

A

W

F

E

P

W

W

G

G

S

A

Y

Y

Y

L

G

G

-

A

D

E

A

A

K

L

T

E

R

Q

G

G

A

I

K

D

L

A

D

M

I

V

A

S

K

S

W

W

K

N

R

R

P

A

S

A

P

P

E

N

T

T

I

I

P

P

V

T

H

A

A

A

K
|
K

A

A

A

G

G

G

L

N

Y
|
Y

M

L

I

S

C

S

T

L

S

V

K

G

H

S

A

E

A

A

E

R

A

R

K

H

G

G

C

Y

S

Q

D

E

A

G

L

I

F

A

M

L

D

D

Y

V

R

N

G

G

Y

Y

V

I

A

S

E
|
E

A

G

T

A

G
|
G

A
x
E

N

N

I

L

F

F

F

E

V

V

K

K

D

D

G

G

E

V

V

L

H

F

T

T

P

P

D

P

-

F

P

T

D

S

C

S

F

A

L
|
L

N

P

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

A

V

I

I

I

G

K

M

L

L

A

R

K

D

E

K

L

Q

G

V

I

K

E

V

V

H

R

E

E

R

Q

H

V

I

L

M

S

P

R

E

E

E

S

L

L

E

Y

G

L

F

A

E

D

Q

E

C

V

W

F

L

M

T

S

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

V

V

G

R

Q

S

I

V

G

D

T

G

Y

I

N

Q

F

V

-

G

-

E

-

G

E

R

V

C

G

G

A

P

L

V

T

T

R

K

D

R

I

I

A

Q

E

Q

S

A

Y

F

active site: K156 (= K158)
binding 4-methyl valeric acid: Y92 (= Y95), K156 (= K158), T254 (= T255), A255 (= A256)
binding pyridoxal-5'-phosphate: R56 (= R59), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), E194 (≠ A196), L214 (= L215), G216 (= G217), I217 (= I218), T218 (= T219), G253 (= G254), T254 (= T255)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
34% identity, 94% coverage: 12:283/289 of query aligns to 5:285/304 of 1i1lA

query
sites
1i1lA

I

I

W

W

M

F

D

N

G

G

K

E

L

M

V

V

D

R

W

W

R

E

A

D

A

A

N

K

V

V

H

H

I

V

L

M

T

S

H

H

A

A

M

L

H

H

Y

Y

A

G

S

T

S

S

V

V

F

F

E

E

G

G

E

I

R

R

A

C

Y

Y

N

D

G

S

K

H

-

K

-

G

-

P

-

V

I

V

F

F

E

R

S

H

R

R

R

E

H

H

S

M

E

Q

R
|
R

L

L

H

H

F

D

S

S

A

A

G

K

E

I

L

Y

D

R

I

F

E

P

I

V

P

S

F

Q

T

S

V

I

D

D

E

E

I

L

E

M

A

E

A

A

K

C

Y

R

E

D

V

V

M

I

K

R

V

K

N

N

G

N

L

L

S

T

D

S

A

A

Y
|
Y

V

I

R

R

A

P

V

L

A

I

W

F

R

V

G

G

A

-

G

-

P

-

D

D

M

V

G

G

V

M

A

G

S

V

A

-

R

-

N

N

P

P

-

P

-

A

-

G

-

Y

-

S

V

T

H

D

L

V

A

I

I

I

A

A

C

A

W

F

E

P

W

W

G

G

S

A

Y

Y

Y

L

G

G

-

A

D

E

A

A

K

L

T

E

R

Q

G

G

A

I

K

D

L

A

D

M

I

V

A

S

K

S

W

W

K

N

R

R

P

A

S

A

P

P

E

N

T

T

I

I

P

P

V

T

H

A

A

A

K
|
K

A

A

A

G

G

G

L

N

Y
|
Y

M

L

I

S

C

S

T

L

S

V

K

G

H

S

A

E

A

A

E

R

A

R

K

H

G

G

C

Y

S

Q

D

E

A

G

L

I

F

A

M

L

D

D

Y

V

R

N

G

G

Y

Y

V

I

A

S

E
|
E

A

G

T

A

G
|
G

A

E

N

N

I

L

F

F

F

E

V

V

K

K

D

D

G

G

E

V

V

L

H

F

T

T

P

P

D

P

-

F

P

T

D

S

C

S

F

A

L

L

N

P

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

A

V

I

I

I

G

K

M

L

L

A

R

K

D

E

K

L

Q

G

V

I

K

E

V

V

H

R

E

E

R

Q

H

V

I

L

M

S

P

R

E

E

E

S

L

L

E

Y

G

L

F

A

E

D

Q

E

C

V

W

F

L

M

T

S

G

G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

V

V

G

R

Q

S

I

V

G

D

T

G

Y

I

N

Q

F

V

-

G

-

E

-

G

E

R

V

C

G

G

A

P

L

V

T

T

R

K

D

R

I

I

A

Q

E

Q

S

A

Y

F

active site: K156 (= K158)
binding 2-methylleucine: Y92 (= Y95), K156 (= K158), T254 (= T255), A255 (= A256)
binding pyridoxal-5'-phosphate: R56 (= R59), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), G216 (= G217), I217 (= I218), T218 (= T219), T254 (= T255)

6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
29% identity, 96% coverage: 12:287/289 of query aligns to 6:290/307 of 6q8eA

query
sites
6q8eA

I

L

W

W

M

W

D

N

G

H

K

R

L

I

V

I

D

P

W

W

R

E

A

E

A

A

N

T

V

V

H

H

I

L

L

T

T

D

H

Y

A

W

M

W

H

A

Y

S

A

V

S

T

S

A

V

V

F
|
F

E

E

G

G

E

I

R

R

A

G

Y

Y

N

W

G

N

K

N

-

A

-

E

-

G

-

E

-

M

-

Y

I

I

F

F

E

R

S

L

R

E

R

D

H

H

S

A

E

R

R
|
R

L

L

H

E

F

Q

S

S

A

M

G

Q

E

L

L

I

D

R

I

M

E

P

I

K

P

E

F

F

T

T

V

V

D

D

E

E

I

I

E

C

A

Q

A

A

K

T

Y

I

E

D

V

L

M

V

K

R

V

A

N

N

G

D

L

Y

S

R

-

G

D

D

A

V

Y

Y

V

I

R

M

A

P

V

L

A

A

W

Y

R

A

G

V

A

G

G

N

P

K

D

A

M

F

G

S

V

V

A

V

S

G

A

D

R

R

N

T

P

T

V

-

H

-

L

-

A

-

I

-

A

-

C

-

W

-

E

E

W

M

G

F

S

I

Y

Y

Y

S

G

R

D

P

A

A

K

V

T

S

R

R

-

L

-

E

-

D

-

F

G

S

A

L

K

H

L

A

D

C

I

Y

A

S

K

S

W

W

K

T

R

R

P

I

S

N

P

E

E

R

T

V

I

L

P

P

V

P

H

R

A

I

K
|
K

A

A

A

L

G

A

L

N

Y
|
Y

M

R

I

N

C

S

T

Q

T

L

S

A

K

S

H

S

A

E

A

A

E

A

A

M

K

N

G

G

C

Y

S

D

D

T

A

A

L

L

F

F

M

L

D

N

Y

P

R

E

G

G

Y

K

V

V

A

A

E
|
E

A

G

T

T

G
|
G

A
x
S

N
x
C

I

V

F

F

V

V

K

R

D

K

G

G

E

K

V

L

H

I

T

T

P

P

D

D

-

I

P

T

D

S

C

G

F

I

L
|
L

N

E

G
x
S

I
|
I

T
|
T

R

R

Q

D

T

T

V

V

I

I

G

H

M

L

L

A

R

R

D

E

K

V

Q

L

-

G

V

L

K

E

V

V

H

E

E

E

R

R

H

V

I

V

M

D

P

R

E

T

E

E

L

T

E

Y

G

L

F

A

E

D

Q

E

C

A

W

F

L

L

T

C

G
|
G

T
|
T

A

H

A

A

E

E

V

I

T

T

P

P

V

I

G

A

Q

S

I

I

G

D

T

R

Y

H

N

E

F

M

E

K

V

H

G

G

A

A

-

P

-

G

-

P

L

I

T

T

R

R

D

Q

I

L

A

R

E

D

S

I

Y

Y

E

R

Q

E

L

V

V

V

active site: F34 (= F40), K156 (= K158), E190 (= E192), L214 (= L215)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R59), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), S194 (≠ A196), C195 (≠ N197), L214 (= L215), S216 (≠ G217), I217 (= I218), T218 (= T219), G254 (= G254), T255 (= T255)

6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
28% identity, 94% coverage: 13:283/289 of query aligns to 8:288/308 of 6h65C

query
sites
6h65C

W

W

M

M

D

N

G

G

K

Q

L

C

V

I

D

P

W

W

R

D

A

Q

A

C

N

S

V

L

H

H

I

V

L

S

T

T

H

Q

A

A

M

A

H

F

Y

F

A

G

S

A

S

S

V

L

F
|
F

E

E

G

G

E

V

R

R

A

A

Y

Y

-

W

N

N

G

A

K

E

-

R

-

E

-

Q

-

L

-

Y

I

V

F

F

E

R

S

L

R

D

R

E

H

H

S

L

E

R

R
|
R

L

L

H

E

F

Q

S

S

A

A

G

K

E

M

L

L

D

R

I

M

E

K

I

L

P

S

F

M

T

P

V

I

D

A

E

D

I

I

E

R

A

Q

A

G

K

V

Y

L

E

E

V

L

M

L

K

R

V

A

N

N

G

E

L

F

-

R

S

S

D

D

A

V

Y

H

V

L

R

Y

A

V

V

A

A

S

W

Y

R

F

G

G

A

I

G

N

-

H

-

D

P

P

D

D

-

P

-

L

-

F

-

P

-

T

-

D

-

T

-

G

-

V

-

Y

-

V

M

T

G

G

V

T

A

A

S

V

A

S

R

R

N

L

P

P

-

L

V

V

H

H

L

T

A

G

I

I

-

S

A

A

C

C

W

-

E

-

W

-

G

-

S

-

Y

-

G

-

D

-

A

-

K

-

T

-

R

-

G

-

A

-

K

-

L

-

D

-

I

M

A

S

K

S

W

W

K

R

R

R

P

I

S

S

P

D

E

D

T

S

I

V

P

P

V

P

H

R

A

I

K
|
K

A

I

A

G

G

A

L

N

Y
|
Y

M

Q

I

N

C

S

T

R

T

L

S

A

K

Q

H

T

A

E

A

A

E

R

A

V

K

N

G

G

C

Y

S

H

D

T

A

S

L

V

F

L

M

L

D

N

Y

S

R

R

G

G

Y

K

V

V

A

S

E
|
E

A

T

T

P

G

G

A
|
A

N

C

I

L

F

L

F

M

V

V

K

R

D

D

G

G

E

R

V

V

H

I

T

S

P

P

D

P

P

V

D

T

C

A

-

D

F

I

L
|
L

N

E

G
x
S

I
x
V

T
|
T

R

R

Q

K

T

T

V

L

I

M

G

S

M

L

L

S

R

E

-

A

D

E

K

L

Q

D

V

S

K

P

V

V

H

I

E

E

R

R

H

D

I

M

M

D

P

R

E

T

E

E

L

L

E

Y

G

I

F

A

E

E

Q

E

C

V

W

F

L

L

T

C

G
|
G

T
|
T

A

I

A

A

E

E

V

I

T

L

P

P

V

V

G

T

Q

T

I

I

G

D

T

R

Y

I

N

Q

F

V

-

G

-

D

-

G

E

E

V

V

G

G

A

P

L

V

T

T

R

R

D

R

I

L

A

Q

E

E

S

L

Y

Y

active site: F35 (= F40), K158 (= K158), E192 (= E192), L216 (= L215)
binding pyridoxal-5'-phosphate: R60 (= R59), K158 (= K158), Y163 (= Y163), E192 (= E192), A196 (= A196), L216 (= L215), S218 (≠ G217), V219 (≠ I218), T220 (= T219), G256 (= G254), T257 (= T255)

7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
28% identity, 96% coverage: 12:287/289 of query aligns to 6:290/309 of 7neaA

query
sites
7neaA

I

L

W

W

M

W

D

N

G

H

K

R

L

I

V

I

D

P

W

W

R

E

A

E

A

A

N

T

V

V

H

H

I

L

L

T

T

D

H

Y

A

W

M

W

H

A

Y

S

A

V

S

T

S

A

V

V

F
|
F

E

E

G

V

E

I

R

S

A

G

Y

Y

N

W

G

N

K

N

-

A

-

E

-

G

-

E

-

M

-

Y

I

I

F

F

E

R

S

L

R

E

R

D

H

H

S

A

E

R

R
|
R

L

L

H

E

F

Q

S

S

A

M

G

Q

E

L

L

I

D

R

I

M

E

P

I

K

P

E

F

F

T

T

V

V

D

D

E

E

I

I

E

C

A

Q

A

A

K

T

Y

I

E

D

V

L

M

V

K

R

V

A

N

N

G

D

L

Y

S

R

-

G

D

D

A

V

Y

F

V

I

R

M

A

P

V

L

A

A

W

Y

R

A

G

V

A

G

G

N

P

K

D

A