SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3610306 FitnessBrowser__Dino:3610306 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

D5ARH0 Biotin transport ATP-binding protein BioM; ECF transporter A component BioM; EC 7.6.2.- from Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) (see paper)
41% identity, 98% coverage: 2:218/221 of query aligns to 4:223/234 of D5ARH0

query
sites
D5ARH0

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K41 (= K39) mutation to N: Diminished biotin uptake, loss of ATPase activity, BioMNY complexes still form.

8bmsA Cryo-em structure of the mutant solitary ecf module 2eq in msp2n2 lipid nanodiscs in the atpase closed and atp-bound conformation (see paper)
35% identity, 96% coverage: 1:212/221 of query aligns to 4:227/278 of 8bmsA

query
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binding adenosine-5'-triphosphate: F12 (≠ V9), N39 (= N35), G40 (= G36), S41 (= S37), G42 (= G38), K43 (= K39), S44 (= S40), T45 (= T41), Q88 (= Q77), Y133 (≠ L122), N140 (≠ Q129), S142 (= S131), G143 (= G132), G144 (= G133), Q145 (≠ E134), Q166 (≠ E155), H199 (= H184)
binding magnesium ion: S44 (= S40), Q88 (= Q77)

Q5M243 Energy-coupling factor transporter ATP-binding protein EcfA1; ECF transporter A component EcfA1; EC 7.-.-.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
39% identity, 82% coverage: 29:209/221 of query aligns to 33:221/276 of Q5M243

query
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Q5M243

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Q90 (= Q82) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-97 in EcfA2.
E163 (= E155) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-171 in EcfA2.

8bmpA Cryo-em structure of the folate-specific ecf transporter complex in msp2n2 lipid nanodiscs bound to atp and adp (see paper)
35% identity, 96% coverage: 1:212/221 of query aligns to 5:227/278 of 8bmpA

query
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8bmpA

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binding adenosine-5'-triphosphate: F13 (≠ V9), N39 (= N35), G42 (= G38), K43 (= K39), S44 (= S40), T45 (= T41)

5x40A Structure of a cbio dimer bound with amppcp (see paper)
36% identity, 92% coverage: 12:215/221 of query aligns to 16:230/280 of 5x40A

query
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5x40A

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binding phosphomethylphosphonic acid adenylate ester: V18 (≠ R14), A20 (≠ I16), N40 (= N35), G41 (= G36), G43 (= G38), K44 (= K39), S45 (= S40), T46 (= T41), Q88 (= Q77), H139 (= H128), M140 (≠ Q129), L141 (= L130), S142 (= S131), G144 (= G133), Q145 (≠ E134), Q166 (≠ E155), H198 (= H184)
binding magnesium ion: S45 (= S40), Q88 (= Q77)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 14

5d3mA Folate ecf transporter: amppnp bound state (see paper)
35% identity, 96% coverage: 1:212/221 of query aligns to 5:230/280 of 5d3mA

query
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5d3mA

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A

A

F

F

G

G

L

L

K

E

N

N

L

R

K

A

L

V

P

P

S

R

A

P

T

E

I

M

K

L

E

K

R

I

I

V

T

A

E

Q

V

A

M

V

A

A

R

D

F

V

Q

G

I

M

E

A

H

D

L

Y

K

A

D

D

R

S

L

E

T

P

H

S

Q

N

L

L

S

S

G

G

E

Q

R

K

Q

Q

M

R

V

V

A

A

L

I

A

A

G

G

V

I

L

L

V

A

M

V

Q

K

P

P

Q

Q

M

V

I

I

V

I

F

L

D

D

E

E

P

S

T

T

T

S

L

M

L

L

D

D

L

P

W

E

N

G

K

K

K

E

R

Q

L

I

M

L

R

D

A

L

I

V

A

R

D

K

L

I

K

K

E

E

H

D

-

N

-

L

-

T

V

V

V

I

L

S

V

I

S

T

H

H

D

D

L

L

D

E

L

E

L

A

R

A

E

G

F

A

D

D

R

Q

V

V

I

L

H

V

I

L

E

D

A

D

G

G

R

Q

I

L

V

L

G

D

D

Q

G

G

E

K

P

P

N

E

A

E

V

I

binding phosphoaminophosphonic acid-adenylate ester: F13 (vs. gap), N42 (= N35), G43 (= G36), K46 (= K39), S47 (= S40)

Q5M244 Energy-coupling factor transporter ATP-binding protein EcfA2; ECF transporter A component EcfA2; EC 3.6.3.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
35% identity, 90% coverage: 14:213/221 of query aligns to 20:233/280 of Q5M244

query
sites
Q5M244

R

R

P

R

I

A

L

L

S

F

D

D

I

M

N

T

I

V

D

T

L

I

N

K

E

D

-

G

K

S

R

Y

I

T

G

A

I

F

I

I

G

G

R

H

N

T

G

G

S

S

G

G

K

K

S

S

T

T

F

I

A

M

R

Q

L

L

F

L

N

N

G

G

L

L

E

Y

K

L

P

P

S

T

S

S

G

G

S

Q

I

V

T

K

L

V

E

D

G

D

S

T

-

I

-

N

-

S

-

Q

-

S

-

K

-

N

A

K

E

E

L

I

K

K

D

P

L

I

R

R

R

K

K

K

V

V

G

G

F

L

V

V

F

F

Q

Q

N

F

P

P

D

E

N

S

Q
|
Q

I

L

V

F

Y

A

P

E

I

T

V

V

D

L

E

E

D

D

L

I

A

A

F

F

G

G

L

P

K

Q

N

N

L

F

K

G

L

V

P

S

S

K

A

E

T

E

I

A

K

E

E

Q

R

R

I

A

T

L

E

E

V

S

M

L

A

R

R

L

F

V

Q

G

I

L

-

S

E

D

H

E

L

L

K

R

D

D

R

Q

L

N

T

P

H

F

Q

D

L

L

S

S

G

G

E

Q

R

M

Q

R

M

R

V

V

A

A

L

I

A

A

G

G

V

I

L

L

V

A

M

M

Q

Q

P

P

Q

D

M

I

I

L

V

V

F

L

D

D

E
|
E

P

P

T

T

T

A

L

G

L

L

D

D

L

P

W

Q

N

G

K

R

K

K

R

E

L

L

M

M

R

S

A

L

I

F

A

K

D

Q

L

L

K

H

E

L

H

S

-

G

-

I

-

T

V

I

V

V

L

L

V

V

S

T

H

H

D

L

L

M

D

D

L

D

L

V

R

A

E

D

F

Y

D

A

R

T

V

A

I

V

H

N

I

V

-

M

E

E

A

K

G

G

R

R

I

L

V

V

G

L

D

S

G

G

E

T

P

P

N

K

A

D

V

V

I

F

Q97 (= Q82) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-90 in EcfA1.
E171 (= E155) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-163 in EcfA1.

4hluC Structure of the ecfa-a' heterodimer bound to adp (see paper)
33% identity, 91% coverage: 2:203/221 of query aligns to 5:210/249 of 4hluC

query
sites
4hluC

L

I

S

E

F

L

S

N

Q

S

V

V

V

S

V

F

E

R

K
x
Y

G

N

G

G

R

D

P

Y

I

V

L

L

S

K

D

D

I

V

N

N

I

A

D

E

L

F

N

E

E

T

K

G

R

K

I

I

G

Y

I

V

-

V

G

G

R

K

N
|
N

G
|
G

S

S

G
|
G

K
|
K

S
x
T

T
|
T

F

L

A

L

R

K

L

I

F

L

N

A

G

G

L

L

E

-

K

L

P

A

S

A

G

G

S

E

I

I

T

F

L

L

E

D

G

G

S

S

-

P

A

A

E

D

L

P

K

F

D

L

L

L

R

R

R

K

K

N

V

V

G

G

F

Y

V

V

F

F

Q

Q

N

N

P

P

D

S

N

S

Q

Q

I

I

V

I

Y

G

P

A

I

T

V

V

D

E

E

E

D

D

L

V

A

A

F

F

G

S

L

L

K

E

N

I

L

M

K

G

L

L

P

D

S

E

A

S

T

E

I

M

K

R

E

K

R

R

I

I

T

K

E

K

V

V

M

L

A

E

R

L

F

V

Q

G

I

L

E

S

H

G

L

L

K

A

D

A

R

A

L

D

T

P

H

L

Q

N

L

L

S

S

G

G

E

Q

R

K

Q

Q

M

R

V

L

A

A

L

I

A

A

G

S

V

M

L

L

V

A

M

R

Q

D

P

T

Q

R

M

F

I

L

V

A

F

L

D

D

E

E

P

P

T

V

T

S

L

M

L

L

D

D

L

P

W

P

N

S

K

Q

K

R

R

E

L

I

M

F

R

Q

A

V

I

L

A

E

D

S

L

L

K

K

E

N

H

E

-

G

-

K

-

G

V

I

L

L

V

V

S

T

H

H

D

E

L

L

D

E

L

Y

L

L

R

D

E

D

F

M

D

D

R

F

V

I

I

L

H

H

I

I

E

S

A

N

G

G

R

T

I

I

binding adenosine-5'-diphosphate: Y14 (≠ K11), N39 (= N35), G40 (= G36), G42 (= G38), K43 (= K39), T44 (≠ S40), T45 (= T41)

4zirB Crystal structure of ecfaa' heterodimer bound to amppnp (see paper)
32% identity, 91% coverage: 2:203/221 of query aligns to 4:206/247 of 4zirB

query
sites
4zirB

L

I

S

E

F

L

S

N

Q

S

V

V

V

S

V

F

E

R

K
x
Y

G

N

G

G

R

D

P

Y

I
x
V

L

L

S

K

D

D

I

V

N

N

I

A

D

E

L

F

N

E

E

T

K

G

R

K

I

I

G

Y

I

V

-

V

G

G

R

K

N
|
N

G
|
G

S

S

G
|
G

K
|
K

S
x
T

T
|
T

F

L

A

L

R

K

L

I

F

L

N

A

G

G

L

L

E

-

K

L

P

A

S

A

G

G

S

E

I

I

T

F

L

L

E

D

G

G

S

S

-

P

A

A

E

D

L

P

K

F

D

L

L

L

R

R

R

K

K

N

V

V

G

G

F

Y

V

V

F

F

Q
|
Q

N

N

P

P

D

S

N

S

Q

Q

I

I

V

I

Y

G

P

A

I

T

V

V

D

E

E

E

D

D

L

V

A

A

F

F

G

-

L

-

K

-

N

S

L

L

K

E

L

I

P

D

S

E

A

S

T

E

I

M

K

R

E

K

R

R

I

I

T

K

E

K

V

V

M

L

A

E

R

L

F

V

Q

G

I

L

E

S

H

G

L

L

K

A

D

A

R

A

L

D

T

P

H

L

Q
x
N

L

L

S
|
S

G
|
G

E

Q

R

K

Q

Q

M

R

V

L

A

A

L

I

A

A

G

S

V

M

L

L

V

A

M

R

Q

D

P

T

Q

R

M

F

I

L

V

A

F

L

D

D

E

E

P

P

T

V

T

S

L

M

L

L

D

D

L

P

W

P

N

S

K

Q

K

R

R

E

L

I

M

F

R

Q

A

V

I

L

A

E

D

S

L

L

K

K

E

N

H

E

-

G

-

K

-

G

V

I

L

L

V

V

S

T

H

H

D

E

L

L

D

E

L

Y

L

L

R

D

E

D

F

M

D

D

R

F

V

I

I

L

H

H

I

I

E

S

A

N

G

G

R

T

I

I

binding phosphoaminophosphonic acid-adenylate ester: Y13 (≠ K11), V18 (≠ I16), N38 (= N35), G39 (= G36), G41 (= G38), K42 (= K39), T43 (≠ S40), T44 (= T41), Q80 (= Q77), N129 (≠ Q129), S131 (= S131), G132 (= G132), G133 (= G133)
binding magnesium ion: T43 (≠ S40), Q80 (= Q77)

4zirA Crystal structure of ecfaa' heterodimer bound to amppnp (see paper)
33% identity, 92% coverage: 17:219/221 of query aligns to 23:230/263 of 4zirA

query
sites
4zirA

L

L

S

E

D

N

I

V

N

S

I

L

D

V

L

I

N

N

E

E

K

G

R

E

I

C

G

L

I

L

I

V

-

A

G

G

R

N

N
x
T

G

G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

A

L

R

Q

L

I

F

V

N

A

G

G

L

L

E

I

K

E

P

P

S

T

S

S

G

G

S

D

I

V

T

L

L

Y

E

D

G

G

S

E

A

K

E

G

L

Y

K

-

D

E

L

I

R

R

K

N

V

I

G

G

F

I

V

A

F

F

Q
|
Q

N

Y

P

P

D

E

N

D

Q

Q

I

F

V

F

Y

A

P

E

I

R

V

V

D

F

E

D

D

E

L

V

A

A

F

F

G

A

L

V

K

K

N

N

L

F

-

Y

-

P

-

D

K

R

L

D

P

P

S

V

A

P

T

L

I

V

K

K

E

K

R

A

I

M

T

E

E

F

V

V

M

G

A

L

R

D

F

F

Q

-

I

-

E

D

H

S

L

F

K

K

D

D

R
|
R

L

V

T

P

H

F

Q
x
F

L

L

S
|
S

G
|
G

E
|
E

R

K

Q

R

M

R

V

V

A

A

L

I

A

A

G

S

V

V

L

I

V

V

M

H

Q

E

P

P

Q

D

M

I

I

L

V

I

F

L

D
|
D

E

E

P

P

T

L

T

V

L

G

L

L

D

D

L

R

W

E

N

G

K

K

K

T

R

D

L

L

M

L

R

R

A

I

I

V

A

E

D

K

L

W

K

K

-

T

-

L

-

G

E

K

H

T

V

V

V

I

L

L

V

I

S

S

H

H

D

D

L

I

D

E

-

T

L

V

L

I

R

N

E

H

F

V

D

D

R

R

V

V

I

V

H

V

I

L

E

E

A

K

G

G

R

K

I

K

V

V

G

F

D

D

G

G

E

T

P

R

N

M

A

E

V

F

I

L

G

E

D

K

Y

Y

I

D

A

P

R

R

binding phosphoaminophosphonic acid-adenylate ester: T42 (≠ N35), G45 (= G38), K46 (= K39), S47 (= S40), T48 (= T41), Q83 (= Q77), R132 (= R125), F136 (≠ Q129), S138 (= S131), G139 (= G132), G140 (= G133), E141 (= E134)
binding magnesium ion: S47 (= S40), Q83 (= Q77), D161 (= D154)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 12

4hluA Structure of the ecfa-a' heterodimer bound to adp (see paper)
33% identity, 92% coverage: 17:219/221 of query aligns to 23:232/265 of 4hluA

query
sites
4hluA

L

L

S

E

D

N

I

V

N

S

I

L

D

V

L

I

N

N

E

E

K

G

R

E

I

C

G

L

I

L

I

V

-

A

G

G

R

N

N

T

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

A

L

R

Q

L

I

F

V

N

A

G

G

L

L

E

I

K

E

P

P

S

T

S

S

G

G

S

D

I

V

T

L

L

Y

E

D

G

G

S

E

A

R

E

K

L

K

-

G

K

Y

D

E

L

I

R

R

K

N

V

I

G

G

F

I

V

A

F

F

Q

Q

N

Y

P

P

D

E

N

D

Q

Q

I

F

V

F

Y

A

P

E

I

R

V

V

D

F

E

D

D

E

L

V

A

A

F

F

G

A

L

V

K

K

N

N

L

F

-

Y

-

P

-

D

K

R

L

D

P

P

S

V

A

P

T

L

I

V

K

K

E

K

R

A

I

M

T

E

E

F

V

V

M

G

A

L

R

D

F

F

Q

-

I

-

E

D

H

S

L

F

K

K

D

D

R

R

L

V

T

P

H

F

Q

F

L

L

S

S

G

G

E

E

R

K

Q

R

M

R

V

V

A

A

L

I

A

A

G

S

V

V

L

I

V

V

M

H

Q

E

P

P

Q

D

M

I

I

L

V

I

F

L

D

D

E

E

P

P

T

L

T

V

L

G

L

L

D

D

L

R

W

E

N

G

K

K

K

T

R

D

L

L

M

L

R

R

A

I

I

V

A

E

D

K

L

W

K

K

-

T

-

L

-

G

E

K

H

T

V

V

V

I

L

L

V

I

S

S

H

H

D

D

L

I

D

E

-

T

L

V

L

I

R

N

E

H

F

V

D

D

R

R

V

V

I

V

H

V

I

L

E

E

A

K

G

G

R

K

I

K

V

V

G

F

D

D

G

G

E

T

P

R

N

M

A

E

V

F

I

L

G

E

D

K

Y

Y

I

D

A

P

R

R

binding adenosine-5'-diphosphate: G43 (= G36), S44 (= S37), G45 (= G38), K46 (= K39), S47 (= S40), T48 (= T41)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 20

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
33% identity, 91% coverage: 8:209/221 of query aligns to 12:225/353 of 1oxvD

query
sites
1oxvD

V

V

V
x
F

E

K

K

K

G

G

G

K

R

V

P

V

I

A

L

L

S

D

D

N

I

V

N

N

I

I

D

N

L

I

-

E

N

N

E

G

K

E

R

R

I

F

G

G

I

I

I

L

G

G

R

P

N
x
S

G
|
G

S

A

G
|
G

K
|
K

S
x
T

T
|
T

F

F

A

M

R

R

L

I

F

I

N

A

G

G

L

L

E

D

K

V

P

P

S

S

S

T

G

G

S

E

I

L

T

Y

L

F

E

D

-

D

-

R

-

L

-

V

-

A

-

S

-

N

G

G

S

K

A

L

E

I

L

V

K

P

D

P

L

E

R

D

R

R

K

K

V

I

G

G

F

M

V

V

F

F

Q
|
Q

N

T

P

-

D

-

N

-

Q

W

I

A

V

L

Y

Y

P

P

I

N

V

L

D

T

-

A

-

F

E

E

D

N

L

I

A

A

F

F

G

P

L

L

K

T

N

N

L

M

K

K

L

M

P

S

S

K

A

E

T

E

I

I

K

R

E

K

R

R

I

V

T

E

E

E

V

V

M

A

A

K

R

I

F

L

Q

D

I

I

E

H

H

H

L

V

K

L

D

N

R

H

L

F

T

P

H

R

Q

E

L

L

S

S

G

G

E

Q

R

Q

Q

Q

M

R

V

V

A

A

L

L

A

A

G

R

V

A

L

L

V

V

M

K

Q

D

P

P

Q

S

M

L

I

L

V

L

F

L

D

D

E
|
E

P

P

T

F

T

S

L

N

L

L

D

D

L

A

W

R

N

M

K

R

K

D

R

S

L

A

M

R

R

A

A

L

I

V

A

K

D

E

L

V

K

Q

E

S

H

R

-

L

-

G

-

V

-

T

V

L

L

V

V

V

S

S

H

H

D

D

-

P

L

A

D

D

L

I

L

F

R

A

E

I

F

A

D

D

R

R

V

V

I

G

H

V

I

L

E

V

A

K

G

G

R

K

I

L

V

V

G

Q

D

V

G

G

E

K

P

P

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ V9), S40 (≠ N35), G41 (= G36), G43 (= G38), K44 (= K39), T45 (≠ S40), T46 (= T41), Q89 (= Q77), E166 (= E155)
binding magnesium ion: T45 (≠ S40), Q89 (= Q77)

1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
33% identity, 91% coverage: 8:209/221 of query aligns to 12:225/353 of 1oxvA

query
sites
1oxvA

V

V

V
x
F

E

K

K

K

G

G

G

K

R

V

P

V

I

A

L

L

S

D

D

N

I

V

N

N

I

I

D

N

L

I

-

E

N

N

E

G

K

E

R

R

I

F

G

G

I

I

I

L

G

G

R

P

N
x
S

G
|
G

S

A

G
|
G

K
|
K

S
x
T

T
|
T

F

F

A

M

R

R

L

I

F

I

N

A

G

G

L

L

E

D

K

V

P

P

S

S

S

T

G

G

S

E

I

L

T

Y

L

F

E

D

-

D

-

R

-

L

-

V

-

A

-

S

-

N

G

G

S

K

A

L

E

I

L

V

K

P

D

P

L

E

R

D

R

R

K

K

V

I

G

G

F

M

V

V

F

F

Q

Q

N

T

P

-

D

-

N

-

Q

W

I

A

V

L

Y

Y

P

P

I

N

V

L

D

T

-

A

-

F

E

E

D

N

L

I

A

A

F

F

G

P

L

L

K

T

N

N

L

M

K

K

L

M

P

S

S

K

A

E

T

E

I

I

K

R

E

K

R

R

I

V

T

E

E

E

V

V

M

A

A

K

R

I

F

L

Q

D

I

I

E

H

H

H

L

V

K

L

D

N

R

H

L

F

T

P

H

R

Q

E

L

L

S

S

G

G

E

Q

R

Q

Q

Q

M

R

V

V

A

A

L

L

A

A

G

R

V

A

L

L

V

V

M

K

Q

D

P

P

Q

S

M

L

I

L

V

L

F

L

D

D

E
|
E

P

P

T

F

T

S

L

N

L

L

D

D

L

A

W

R

N

M

K

R

K

D

R

S

L

A

M

R

R

A

A

L

I

V

A

K

D

E

L

V

K

Q

E

S

H

R

-

L

-

G

-

V

-

T

V

L

L

V

V

V

S

S

H

H

D

D

-

P

L

A

D

D

L

I

L

F

R

A

E

I

F

A

D

D

R

R

V

V

I

G

H

V

I

L

E

V

A

K

G

G

R

K

I

L

V

V

G

Q

D

V

G

G

E

K

P

P

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ V9), S40 (≠ N35), G41 (= G36), G43 (= G38), K44 (= K39), T45 (≠ S40), T46 (= T41), E166 (= E155)

1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
33% identity, 91% coverage: 8:209/221 of query aligns to 12:225/353 of 1oxuA

query
sites
1oxuA

V

V

V
x
F

E

K

K

K

G

G

G

K

R

V

P

V

I
x
A

L

L

S

D

D

N

I

V

N

N

I

I

D

N

L

I

-

E

N

N

E

G

K

E

R

R

I

F

G

G

I

I

I

L

G

G

R

P

N

S

G
|
G

S

A

G
|
G

K
|
K

S
x
T

T
|
T

F

F

A

M

R

R

L

I

F

I

N

A

G

G

L

L

E

D

K

V

P

P

S

S

S

T

G

G

S

E

I

L

T

Y

L

F

E

D

-

D

-

R

-

L

-

V

-

A

-

S

-

N

G

G

S

K

A

L

E

I

L

V

K

P

D

P

L

E

R

D

R

R

K

K

V

I

G

G

F

M

V

V

F

F

Q

Q

N

T

P

-

D

-

N

-

Q

W

I

A

V

L

Y

Y

P

P

I

N

V

L

D

T

-

A

-

F

E

E

D

N

L

I

A

A

F

F

G

P

L

L

K

T

N

N

L

M

K

K

L

M

P

S

S

K

A

E

T

E

I

I

K

R

E

K

R

R

I

V

T

E

E

E

V

V

M

A

A

K

R

I

F

L

Q

D

I

I

E

H

H

H

L

V

K

L

D

N

R

H

L

F

T

P

H

R

Q

E

L

L

S

S

G

G

E

Q

R

Q

Q

Q

M

R

V

V

A

A

L

L

A

A

G

R

V

A

L

L

V

V

M

K

Q

D

P

P

Q

S

M

L

I

L

V

L

F

L

D

D

E

E

P

P

T

F

T

S

L

N

L

L

D

D

L

A

W

R

N

M

K

R

K

D

R

S

L

A

M

R

R

A

A

L

I

V

A

K

D

E

L

V

K

Q

E

S

H

R

-

L

-

G

-

V

-

T

V

L

L

V

V

V

S

S

H

H

D

D

-

P

L

A

D

D

L

I

L

F

R

A

E

I

F

A

D

D

R

R

V

V

I

G

H

V

I

L

E

V

A

K

G

G

R

K

I

L

V

V

G

Q

D

V

G

G

E

K

P

P

binding adenosine-5'-diphosphate: F13 (≠ V9), A20 (≠ I16), G41 (= G36), G43 (= G38), K44 (= K39), T45 (≠ S40), T46 (= T41)

Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
33% identity, 91% coverage: 8:209/221 of query aligns to 12:225/353 of Q97UY8

query
sites
Q97UY8

V

V

V

F

E

K

K

K

G

G

G

K

R

V

P

V

I

A

L

L

S

D

D

N

I

V

N

N

I

I

D

N

L

I

-

E

N

N

E

G

K

E

R

R

I

F

G

G

I

I

I

L

G

G

R

P

N

S

G

G

S

A

G

G

K

K

S

T

T

T

F

F

A

M

R

R

L

I

F

I

N

A

G

G

L

L

E

D

K

V

P

P

S

S

S

T

G

G

S

E

I

L

T

Y

L

F

E

D

-

D

-

R

-

L

-

V

-

A

-

S

-

N

G

G

S

K

A

L

E

I

L

V

K

P

D

P

L

E

R

D

R

R

K

K

V

I

G

G

F

M

V

V

F

F

Q

Q

N

T

P

-

D

-

N

-

Q

W

I

A

V

L

Y

Y

P

P

I

N

V

L

D

T

-

A

-

F

E

E

D

N

L

I

A

A

F

F

G

P

L

L

K

T

N

N

L

M

K

K

L

M

P

S

S

K

A

E

T

E

I

I

K

R

E

K

R

R

I

V

T

E

E

E

V

V

M

A

A

K

R

I

F

L

Q

D

I

I

E

H

H

H

L

V

K

L

D

N

R

H

L

F

T

P

H

R

Q

E

L

L

S
|
S

G

G

G
|
G

E

Q

R

Q

Q

Q

M

R

V

V

A

A

L

L

A

A

G

R

V

A

L

L

V

V

M

K

Q

D

P

P

Q

S

M

L

I

L

V

L

F

L

D

D

E
|
E

P

P

T

F

T

S

L

N

L

L

D

D

L

A

W

R

N

M

K

R

K

D

R

S

L

A

M

R

R

A

A

L

I

V

A

K

D

E

L

V

K

Q

E

S

H

R

-

L

-

G

-

V

-

T

V

L

L

V

V

V

S

S

H

H

D

D

-

P

L

A

D

D

L

I

L

F

R

A

E

I

F

A

D

D

R

R

V

V

I

G

H

V

I

L

E

V

A

K

G

G

R

K

I

L

V

V

G

Q

D

V

G

G

E

K

P

P

S142 (= S131) mutation to A: Decrease in ATPase activity. Can form dimers.
G144 (= G133) mutation to A: Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166.
E166 (= E155) mutation to A: Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144.; mutation to Q: Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+).

8bmpB Cryo-em structure of the folate-specific ecf transporter complex in msp2n2 lipid nanodiscs bound to atp and adp (see paper)
35% identity, 90% coverage: 17:215/221 of query aligns to 22:234/281 of 8bmpB

query
sites
8bmpB

L

L

S

D

D

Q

I

L

N

N

I

F

D

S

L

L

N

E

E

E

-

G

K

K

R

F

I

I

G

A

I

L

I

V

G

G

R

H

N

T

G
|
G

S

S

G
|
G

K

K

S
|
S

T
|
T

F

L

A

M

R

Q

L

H

F

F

N

N

G

A

L

L

E

L

K

K

P

P

S

T

S

S

G

G

S

K

I

I

T

E

L

I

E

A

G

G

-

Y

-

T

-

I

-

T

-

P

-

E

-

T

-

G

S

N

A

K

E

G

L

L

K

K

D

D

L

L

R

R

K

K

V

V

G

S

F

L

V

A

F

F

Q

Q

N

F

P

S

D

E

N

A

Q

Q

I

L

V

F

Y

E

P

N

I

T

V

V

D

L

E

K

D

D

L

V

A

E

F

Y

G

G

L

P

K

R

N

N

L

F

K

G

L

F

P

S

S

E

A

D

T

E

I

A

K

R

E

E

R

A

I

A

T

L

E

K

V

W

M

L

A

K

R

K

F

V

Q

G

I

-

E

-

H

-

L

L

K

K

D

D

R

D

L

L

T

I

H

E

Q

H

-

S

-

P

-

F

-

D

L

L

S

S

G

G

E

Q

R

M

Q

R

M

R

V

V

A

A

L

L

A

A

G

G

V

V

L

L

V

A

M

Y

Q

E

P

P

Q

E

M

I

I

I

V

C

F

L

D

D

E

E

P

P

T

A

L

G

L

L

D

D

L

P

W

M

N

G

K

R

K

L

R

E

L

M

M

M

R

Q

A

L

I

F

A

K

D

D

L

Y

K

Q

-

A

-

G

E

H

H

T

V

V

V

I

L

L

V

V

S

T

H

H

D

N

L

M

-

D

D

D

L

V

L

A

R

D

E

Y

F

A

D

D

R

D

V

V

I

L

H

A

I

L

E

E

A

H

G

G

R

R

I

L

V

I

G

K

D

H

G

A

E

S

P

P

N

K

A

E

V

V

I

F

G

K

D

D

binding adenosine-5'-diphosphate: G42 (= G36), G44 (= G38), S46 (= S40), T47 (= T41)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 11

5d3mB Folate ecf transporter: amppnp bound state (see paper)
35% identity, 90% coverage: 17:215/221 of query aligns to 22:234/281 of 5d3mB

query
sites
5d3mB

L

L

S

D

D

Q

I

L

N

N

I

F

D

S

L

L

N

E

E

E

-

G

K

K

R

F

I

I

G

A

I

L

I

V

G

G

R

H

N
x
T

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T

T

F

L

A

M

R

Q

L

H

F

F

N

N

G

A

L

L

E

L

K

K

P

P

S

T

S

S

G

G

S

K

I

I

T

E

L

I

E

A

G

G

-

Y

-

T

-

I

-

T

-

P

-

E

-

T

-

G

S

N

A

K

E

G

L

L

K

K

D

D

L

L

R

R

K

K

V

V

G

S

F

L

V

A

F

F

Q

Q

N

F

P

S

D

E

N

A

Q

Q

I

L

V

F

Y

E

P

N

I

T

V

V

D

L

E

K

D

D

L

V

A

E

F

Y

G

G

L

P

K

R

N

N

L

F

K

G

L

F

P

S

S

E

A

D

T

E

I

A

K

R

E

E

R

A

I

A

T

L

E

K

V

W

M

L

A

K

R

K

F

V

Q

G

I

-

E

-

H

-

L

L

K

K

D

D

R

D

L

L

T

I

H

E

Q

H

-

S

-

P

-

F

-

D

L

L

S

S

G

G

E

Q

R

M

Q

R

M

R

V

V

A

A

L

L

A

A

G

G

V

V

L

L

V

A

M

Y

Q

E

P

P

Q

E

M

I

I

I

V

C

F

L

D
|
D

E

E

P

P

T

A

L

G

L

L

D

D

L

P

W

M

N

G

K

R

K

L

R

E

L

M

M

M

R

Q

A

L

I

F

A

K

D

D

L

Y

K

Q

-

A

-

G

E

H

H

T

V

V

V

I

L

L

V

V

S

T

H

H

D

N

L

M

-

D

D

D

L

V

L

A

R

D

E

Y

F

A

D

D

R

D

V

V

I

L

H

A

I

L

E

E

A

H

G

G

R

R

I

L

V

I

G

K

D

H

G

A

E

S

P

P

N

K

A

E

V

V

I

F

G

K

D

D

binding phosphoaminophosphonic acid-adenylate ester: T41 (≠ N35), G42 (= G36), S43 (= S37), G44 (= G38), K45 (= K39), S46 (= S40), D169 (= D154)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 11

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
33% identity, 94% coverage: 3:209/221 of query aligns to 12:218/371 of P68187

query
sites
P68187

S

A

F

W

S

G

Q

E

V

V

E

S

K

K

G

-

R

-

P

-

I

-

L

-

S

-

D

D

I

I

N

N

I

L

D

D

L

I

N

H

E

E

K

G

R

E

I

F

G

V

I

V

-

F

I

V

G

G

R

P

N

S

G

G

S

C

G

G

K

K

S

S

T

T

F

L

A

L

R

R

L

M

F

I

N

A

G

G

L

L

E

E

K

T

P

I

S

T

S

S

G

G

S

D

I

L

T

F

L

I

E

-

G

G

S

E

A

K

E

R

L

M

K

N

D

D

L

T

-

P

-

A

R

E

R

R

K

G

V

V

G

G

F

M

V

V

F

F

Q

Q

N

S

P

-

D

-

N

-

Q

Y

I
x
A

V

L

Y

Y

P

P

-

H

-

L

I

S

V

V

D

A

E

E

D

N

L

M

A

S

F

F

G

G

L

L

K

K

N

L

L

A

K

G

L

A

P
x
K

S

K

A

E

T

V

I

I

K

N

E

Q

R

R

I
x
V

T

N

E

Q

V
|
V

M

A

A
x
E

R

V

F

L

Q

Q

I

L

E
x
A

H

H

L

L

K

L

D

D

R

R

L

K

T

P

H

K

Q

A

L

L

S

S

G

G

G
|
G

E

Q

R

R

Q

Q

M

R

V

V

A

A

L

I

A

G

G

R

V

T

L

L

V

V

M

A

Q

E

P

P

Q

S

M

V

I

F

V

L

F

L

D
|
D

E

E

P

P

T

L

T

S

L

N

L

L

D

D

L

A

W

A

N

L

K

R

K

V

R

Q

L

M

M

R

R

I

A

E

I

I

A

S

D

R

L

L

K

H

E

K

H

R

-

L

-

G

-

R

-

T

V

M

V

I

L

Y

V

V

S

T

H

H

D

D

-

Q

L

V

D

E

L

A

L

M

R

T

E

L

F

A

D

D

R

K

V

I

I

V

H

V

I

L

E

D

A

A

G

G

R

R

I

V

V

A

G

Q

D

V

G

G

E

K

P

P

A85 (≠ I83) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ P102) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ I110) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (= V113) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ A115) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ E120) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G133) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D154) mutation to N: Loss of maltose transport but retains ability to repress mal genes.

Sites not aligning to the query:

228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
33% identity, 94% coverage: 3:209/221 of query aligns to 11:217/371 of 3puyA

query
sites
3puyA

S

A

F
x
W

S

G

Q

E

V

V

E

S

K

K

G

-

R

-

P

-

I

-

L

-

S

-

D

D

I

I

N

N

I

L

D

D

L

I

N

H

E

E

K

G

R

E

I

F

G

V

I

V

-

F

I

V

G

G

R

P

N
x
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

F

L

A

L

R

R

L

M

F

I

N

A

G

G

L

L

E

E

K

T

P

I

S

T

S

S

G

G

S

D

I

L

T

F

L

I

E

-

G

G

S

E

A

K

E

R

L

M

K

N

D

D

L

T

-

P

-

A

R

E

R

R

K

G

V

V

G

G

F

M

V

V

F

F

Q
|
Q

N

S

P

-

D

-

N

-

Q

Y

I

A

V

L

Y

Y

P

P

-

H

-

L

I

S

V

V

D

A

E

E

D

N

L

M

A

S

F

F

G

G

L

L

K

K

N

L

L

A

K

G

L

A

P

K

S

K

A

E

T

V

I

I

K

N

E

Q

R

R

I

V

T

N

E

Q

V

V

M

A

A

E

R

V

F

L

Q

Q

I

L

E

A

H

H

L

L

K

L

D

D

R
|
R

L

K

T

P

H

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

E
x
Q

R

R

Q

Q

M

R

V

V

A

A

L

I

A

G

G

R

V

T

L

L

V

V

M

A

Q

E

P

P

Q

S

M

V

I

F

V

L

F

L

D

D

E
|
E

P

P

T

L

T

S

L

N

L

L

D

D

L

A

W

A

N

L

K

R

K

V

R

Q

L

M

M

R

R

I

A

E

I

I

A

S

D

R

L

L

K

H

E

K

H

R

-

L

-

G

-

R

-

T

V

M

V

I

L

Y

V

V

S

T

H
|
H

D

D

-

Q

L

V

D

E

L

A

L

M

R

T

E

L

F

A

D

D

R

K

V

I

I

V

H

V

I

L

E

D

A

A

G

G

R

R

I

V

V

A

G

Q

D

V

G

G

E

K

P

P

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F4), S37 (≠ N35), G38 (= G36), C39 (≠ S37), G40 (= G38), K41 (= K39), S42 (= S40), T43 (= T41), Q81 (= Q77), R128 (= R125), A132 (≠ Q129), S134 (= S131), G136 (= G133), Q137 (≠ E134), E158 (= E155), H191 (= H184)
binding magnesium ion: S42 (= S40), Q81 (= Q77)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
33% identity, 94% coverage: 3:209/221 of query aligns to 11:217/371 of 3puxA

query
sites
3puxA

S

A

F
x
W

S

G

Q

E

V

V

E

S

K

K

G

-

R

-

P

-

I

-

L

-

S

-

D

D

I

I

N

N

I

L

D

D

L

I

N

H

E

E

K

G

R

E

I

F

G

V

I

V

-

F

I

V

G

G

R

P

N
x
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

F

L

A

L

R

R

L

M

F

I

N

A

G

G

L

L

E

E

K

T

P

I

S

T

S

S

G

G

S

D

I

L

T

F

L

I

E

-

G

G

S

E

A

K

E

R

L

M

K

N

D

D

L

T

-

P

-

A

R

E

R

R

K

G

V

V

G

G

F

M

V

V

F

F

Q
|
Q

N

S

P

-

D

-

N

-

Q

Y

I

A

V

L

Y

Y

P

P

-

H

-

L

I

S

V

V

D

A

E

E

D

N

L

M

A

S

F

F

G

G

L

L

K

K

N

L

L

A

K

G

L

A

P

K

S

K

A

E

T

V

I

I

K

N

E

Q

R

R

I

V

T

N

E

Q

V

V

M

A

A

E

R

V

F

L

Q

Q

I

L

E

A

H

H

L

L

K

L

D

D

R
|
R

L

K

T

P

H

K

Q

A

L

L

S
|
S

G

G

G
|
G

E
x
Q

R

R

Q

Q

M

R

V

V

A

A

L

I

A

G

G

R

V

T

L

L

V

V

M

A

Q

E

P

P

Q

S

M

V

I

F

V

L

F

L

D

D

E

E

P

P

T

L

T

S

L

N

L

L

D

D

L

A

W

A

N

L

K

R

K

V

R

Q

L

M

M

R

R

I

A

E

I

I

A

S

D

R

L

L

K

H

E

K

H

R

-

L

-

G

-

R

-

T

V

M

V

I

L

Y

V

V

S

T

H
|
H

D

D

-

Q

L

V

D

E

L

A

L

M

R

T

E

L

F

A

D

D

R

K

V

I

I

V

H

V

I

L

E

D

A

A

G

G

R

R

I

V

V

A

G

Q

D

V

G

G

E

K

P

P

binding adenosine-5'-diphosphate: W12 (≠ F4), G38 (= G36), C39 (≠ S37), G40 (= G38), K41 (= K39), S42 (= S40), T43 (= T41), R128 (= R125), S134 (= S131), Q137 (≠ E134)
binding beryllium trifluoride ion: S37 (≠ N35), G38 (= G36), K41 (= K39), Q81 (= Q77), S134 (= S131), G136 (= G133), H191 (= H184)
binding magnesium ion: S42 (= S40), Q81 (= Q77)

Query Sequence

>3610306 FitnessBrowser__Dino:3610306
MLSFSQVVVEKGGRPILSDINIDLNEKRIGIIGRNGSGKSTFARLFNGLEKPSSGSITLE
GSAELKDLRRKVGFVFQNPDNQIVYPIVDEDLAFGLKNLKLPSATIKERITEVMARFQIE
HLKDRLTHQLSGGERQMVALAGVLVMQPQMIVFDEPTTLLDLWNKKRLMRAIADLKEHVV
LVSHDLDLLREFDRVIHIEAGRIVGDGEPNAVIGDYIARSL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory