SitesBLAST

P24232 Flavohemoprotein; Flavohemoglobin; HMP; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD; EC 1.14.12.17 from Escherichia coli (strain K12) (see 2 papers)
27% identity, 65% coverage: 12:242/356 of query aligns to 158:390/396 of P24232

query
sites
P24232

V

V

R

A

K

K

T

T

I

P

R

R

D

S

A

A

V

L

V

I

V

T

T

S

-

F

-

E

L

L

K

E

P

P

V

V

D

D

G

G

D

A

F

V

G

A

-

E

F

Y

I

R

Q

P

G

G

Q

Q

Y

Y

L

L

T

G

F

V

R

W

-

L

-

K

-

P

R

E

S

G

F

F

D

P

G

H

T

Q

E

E

L

I

R

R

R

Q

S

-

Y

Y

S

S

I

L

C

T

A

R

G

K

R

P

D

D

D

G

G

K

V

G

L

Y

Q

R

V

I

G

A

I

V

K

K

R

R

V

E

E

E

G

G

A

Q

F

V

S

S

T

N

W

W

A

L

N

H

D

N

S

H

L

A

A

N

P

V

G

G

M

D

T

V

L

V

E

K

A

L

M

V

A

A

P

P

M

A

G

G

S

D

F

F

H

F

T

M

P

A

L

V

D

A

P

D

H

D

T

T

P

P

R

V

N

T

Y

L

L

I

A

-

F

-

A

S

G

A

G

G

S

V

G

G

I

Q

T

T

P

P

I

M

L

L

S

A

I

M

L

L

K

D

T

T

V

L

L

A

A

K

R

A

E

G

P

H

G

T

S

A

R

Q

L

V

T

N

L

W

V

F

Y

H

A

A

N

A

R

E

G

N

V

G

N

D

T

V

I

H

M

A

F

F

R

A

E

D

E

E

L

V

E

K

D

E

L

L

K

G

N

Q

L

-

H

S

M

L

G

P

R

R

L

F

T

T

V

A

I

-

H

H

V

T

L

W

E

Y

S

R

D

Q

A

P

Q

S

E

E

I

A

D

D

L

R

F

A

T

K

G

G

R

Q

V

F

D

D

G

S

-

E

-

G

-

L

-

M

-

D

-

L

A

S

K

K

C

L

D

E

A

G

L

A

F

F

A

S

H

-

W

-

I

-

D

-

I

-

D

D

S

P

I

T

D

M

T

Q

A

F

F

Y

I

L

C

C

G

G

P

P

E

V

P

G

M

F

M

M

L

Q

G

F

I

T

A

A

A

K

A

Q

L

L

R

V

A

D

H

L

G

G

M

V

T

K

D

Q

D

E

R

N

I

I

K

H

F

Y

E

E

L

C

F

F

Sites not aligning to the query:

29 mutation Y->E,H: 15 to 35-fold reduction in NO dioxygenase activity.; Y→F: 30-fold reduction in NO dioxygenase activity, and 80-fold increase in the O(2) dissociation rate constant.
95 active site, Charge relay system
135 active site, Charge relay system

1pfdA The solution structure of high plant parsley [2fe-2s] ferredoxin, nmr, 18 structures (see paper)
39% identity, 24% coverage: 271:354/356 of query aligns to 8:90/96 of 1pfdA

query
sites
1pfdA

V

I

T

T

M

P

D

D

G

G

A

E

A

V

R

E

S

-

F

F

A

K

M

C

D

D

K

D

D

D

Q

V

S

Y

I

V

L

L

D

D

A

Q

A

A

L

E

A

E

N

E

A

G

L

I

D

D

A

I

P

P

Y

Y

A
x
S

C
|
C

K

R

A

A

G
|
G

V

S

C
|
C

S

S

T

S

C
|
C

K

A

C

G

K

K

V

V

L

V

E

S

G

G

E

S

V

I

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

E

E

Q

V

M

A

D

R

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

H

S

A

Y

Y

P

P

V

T

T

S

D

D

R

V

V

V

V

I

V

E

T

T

Y

H

binding fe2/s2 (inorganic) cluster: S38 (≠ A302), C39 (= C303), G42 (= G306), C44 (= C308), C47 (= C311), C77 (= C341)

3b2gA Leptolyngbya boryana ferredoxin (see paper)
38% identity, 23% coverage: 274:354/356 of query aligns to 12:92/98 of 3b2gA

query
sites
3b2gA

D

E

G

G

A

L

A

N

R

T

S

T

F

I

A

E

M

V

D

P

K

D

D

D

Q

E

S

Y

I

I

L

L

D

D

A

A

L

E

A

E

N

Q

A

G

L

I

D

D

A

L

P

P

Y

Y

A
x
S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

K

A

C

G

K

K

V

I

L

T

E

A

G

G

E

T

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

A

Q

R

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

S

A

Y

Y

P

P

V

T

T

S

D

D

R

C

V

T

V

I

V

L

T

T

Y

H

binding fe2/s2 (inorganic) cluster: S40 (≠ A302), C41 (= C303), R42 (≠ K304), G44 (= G306), C46 (= C308), C49 (= C311), C79 (= C341)

1ewyC Anabaena pcc7119 ferredoxin:ferredoxin-NADP+-reductase complex (see paper)
38% identity, 23% coverage: 274:354/356 of query aligns to 12:92/98 of 1ewyC

query
sites
1ewyC

D

E

G

G

A

T

A

K

R

H

S

E

F

I

A

E

M

V

D

P

K

D

D

D

Q

E

S

Y

I

I

L

L

D

D

A

A

L

E

A

E

N

Q

A

G

L

Y

D

D

A

L

P

P

Y
x
F

A
x
S

C
|
C

K
x
R

A

A

G
|
G

V
x
A

C
|
C

S

S

T

T

C
|
C

K

A

C

G

K

K

V

L

L

V

E

S

G

G

E

T

V

V

E

D

M

Q

I

S

A

D

N

Q

H
x
S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

A

E

R

A

G

G

Y

Y

V

V

L
|
L

S

T

C
|
C

Q

V

S

A

Y

Y

P

P

V

T

T

S

D

D

R

V

V

V

V

I

V

Q

T

T

Y

H

binding flavin-adenine dinucleotide: A45 (≠ V307), S64 (≠ H326)
binding fe2/s2 (inorganic) cluster: F39 (≠ Y301), S40 (≠ A302), C41 (= C303), R42 (≠ K304), G44 (= G306), A45 (≠ V307), C46 (= C308), C49 (= C311), L77 (= L339), C79 (= C341)

1czpA Anabaena pcc7119 [2fe-2s] ferredoxin in the reduced and oxixized state at 1.17 a (see paper)
38% identity, 23% coverage: 274:354/356 of query aligns to 12:92/98 of 1czpA

query
sites
1czpA

D

E

G

G

A

T

A

K

R

H

S

E

F

I

A

E

M

V

D

P

K

D

D

D

Q

E

S

Y

I

I

L

L

D

D

A

A

L

E

A

E

N

Q

A

G

L

Y

D

D

A

L

P

P

Y
x
F

A

S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

K

A

C

G

K

K

V

L

L

V

E

S

G

G

E

T

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

A

E

R

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

S

A

Y

Y

P

P

V

T

T

S

D

D

R

V

V

V

V

I

V

Q

T

T

Y

H

binding fe2/s2 (inorganic) cluster: F39 (≠ Y301), C41 (= C303), R42 (≠ K304), G44 (= G306), C46 (= C308), C49 (= C311), C79 (= C341)

P0A3C7 Ferredoxin-1; Ferredoxin I from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) (see paper)
38% identity, 23% coverage: 274:354/356 of query aligns to 13:93/99 of P0A3C7

query
sites
P0A3C7

D

E

G

G

A

T

A

K

R

H

S

E

F

I

A

E

M

V

D

P

K

D

D

D

Q

E

S

Y

I

I

L

L

D

D

A

A

L

E

A

E

N

Q

A

G

L

Y

D

D

A

L

P

P

Y

F

A

S

C

C

K
x
R

A

A

G

G

V

A

C

C

S

S

T
|
T

C

C

K

A

C

G

K

K

V

L

L

V

E

S

G

G

E

T

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A
x
F

L

L

E

D

D
|
D

Y
x
D

E

Q

V

I

A

E

R

A

G

G

Y

Y

V

V

L

L

S

T

C

C

Q

V

S

A

Y

Y

P

P

V

T

T

S

D

D

R

V

V

V

V

I

V

Q

T

T

Y

H

R43 (≠ K304) mutation R->A,E,H: No effect on electron transfer.
T49 (= T310) mutation to A: No effect on electron transfer.
F66 (≠ A327) mutation F->A,I: 1000-fold decrease in electron transfer.
D69 (= D330) mutation to K: Small effect on electron transfer.
D70 (≠ Y331) mutation to K: Small effect on electron transfer.

Sites not aligning to the query:

95 E→K: 20000-fold decrease in electron transfer.

1gaqB Crystal structure of the complex between ferredoxin and ferredoxin- NADP+ reductase (see paper)
43% identity, 20% coverage: 285:354/356 of query aligns to 21:90/98 of 1gaqB

query
sites
1gaqB

D

D

Q

V

S

Y

I

I

L

L

D

D

A

Q

A

A

L

E

A

E

N

D

A

G

L

I

D

D

A

L

P

P

Y

Y

A

S

C
|
C

K
x
R

A

A

G
|
G

V

S

C
|
C

S

S

T

S

C
|
C

K

A

C

G

K

K

V

V

L

V

E

S

G

G

E

S

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

Y

L

L

E

D

D

D

Y

G

E

Q

V

I

A

A

R

D

G

G

Y

W

V

V

L
|
L

S

T

C
|
C

Q

H

S

A

Y

Y

P

P

V

T

T

S

D

D

R

V

V

V

V

I

V

E

T

T

Y

H

binding fe2/s2 (inorganic) cluster: C39 (= C303), R40 (≠ K304), G42 (= G306), C44 (= C308), C47 (= C311), L75 (= L339), C77 (= C341)

6yacN Plant psi-ferredoxin supercomplex (see paper)
42% identity, 24% coverage: 271:354/356 of query aligns to 8:90/97 of 6yacN

query
sites
6yacN

V

V

T

T

M

P

D

D

G

G

A

T

A

-

R

Q

S

E

F

F

A

E

M

C

D

P

K

S

D

D

Q

V

S

Y

I

I

L

L

D

D

A

H

A

A

L

E

A

E

N

V

A

G

L

I

D

D

A

L

P

P

Y
|
Y

A
x
S

C
|
C

K
x
R

A

A

G

G

V

S

C
|
C

S

S

T
x
S

C
|
C

K

A

C

G

K

K

V

V

L

V

E

G

G

G

E

E

V

V

E

D

M

Q

I

S

A

D

N

G

H

S

A

F

L

L

E

D

D

D

Y

E

E

Q

V

I

A

E

R

A

G

G

Y

F

V

V

L

L

S

T

C
|
C

Q

V

S

A

Y

Y

P

P

V

T

T

S

D

D

R

V

V

V

V

I

V

E

T

T

Y

H

binding fe2/s2 (inorganic) cluster: Y37 (= Y301), S38 (≠ A302), C39 (= C303), R40 (≠ K304), C44 (= C308), S46 (≠ T310), C47 (= C311), C77 (= C341)

1awdA Ferredoxin [2fe-2s] oxidized form from chlorella fusca (see paper)
42% identity, 21% coverage: 284:356/356 of query aligns to 18:90/94 of 1awdA

query
sites
1awdA

K

E

D

D

Q

T

S

Y

I

I

L

L

D

D

A

A

L

E

A

E

N

A

A

G

L

L

D

D

A

L

P

P

Y
|
Y

A

S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

S

C
|
C

K

A

C

G

K

K

V

V

L

E

E

S

G

G

E

E

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

A

E

Q

V

M

A

G

R

K

G

G

Y

F

V

V

L

L

S

T

C
|
C

Q

V

S

A

Y

Y

P

P

V

T

T

S

D

D

R

V

V

T

V

I

V

L

T

T

Y

H

D

Q

H

E

binding fe2/s2 (inorganic) cluster: Y35 (= Y301), C37 (= C303), R38 (≠ K304), G40 (= G306), C42 (= C308), C45 (= C311), C75 (= C341)

Query Sequence

>3610441 FitnessBrowser__Dino:3610441
MARFHPLSVTDVRKTIRDAVVVTLKPVDGGDFGFIQGQYLTFRRSFDGTELRRSYSICAG
RDDGVLQVGIKRVEGGAFSTWANDSLAPGMTLEAMAPMGSFHTPLDPHTPRNYLAFAGGS
GITPILSILKTVLAREPGSRLTLVYANRGVNTIMFREELEDLKNLHMGRLTVIHVLESDA
QEIDLFTGRVDGAKCDALFAHWIDIDSIDTAFICGPEPMMLGIAAALRAHGMTDDRIKFE
LFASGQPGRLPRKPGAAAGHDPEARATAATVTMDGAARSFAMDKDQSILDAALANALDAP
YACKAGVCSTCKCKVLEGEVEMIANHALEDYEVARGYVLSCQSYPVTDRVVVTYDH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory