SitesBLAST

P13866 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Homo sapiens (Human) (see 6 papers)
33% identity, 92% coverage: 12:500/533 of query aligns to 28:567/664 of P13866

query
sites
P13866

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N51 (≠ E35) to S: in GGM; slightly decreased activity; dbSNP:rs17683011
W67 (= W50) mutation to A: Strong reduction in D-glucose transporter activity.
S77 (= S60) mutation to A: Loss of activity.
H83 (≠ S66) mutation to L: Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with A-287 and C-290.; mutation to Q: Loss of D-glucose transporter activity.
R135 (= R118) to W: in GGM; loss of activity
S159 (≠ A141) to P: in GGM; loss of activity
A166 (≠ G148) to T: in GGM; about 90% reduction in activity
D204 (= D187) mutation to A: Loss of activity.
N248 (≠ H223) modified: carbohydrate, N-linked (GlcNAc...) asparagine; mutation to Q: Loss of N-glycosylation.
C255 (≠ L226) modified: Disulfide link with 511
W276 (= W237) to L: in GGM; about 95% reduction in activity
T287 (≠ G247) mutation to A: Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with L-83 and C-290.; mutation to N: Loss of D-glucose transporter activity. Has strict selectivity for D-galactose.; mutation T->S,A: Has normal D-glucose and D-galactose transporter activity.
Y290 (= Y250) mutation to C: Loss of D-galactose transporter activity. Has strict selectivity for D-glucose. Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with A-287 and L-83.
W291 (= W251) mutation to A: Loss of D-glucose transporter activity.
C292 (≠ T252) to Y: in GGM; loss of activity; mutation to A: Has no effect on water permeability.
Q295 (= Q255) to R: in GGM; loss of activity
R300 (= R260) to S: in GGM; loss of activity
A304 (= A264) to V: in GGM; impairs trafficking to the plasma membrane
K321 (= K281) mutation to Q: Acquires D-mannose and D-allose transporter activity comparable to glucose and galactose.
C345 (vs. gap) modified: Disulfide link with 351
C351 (vs. gap) modified: Disulfide link with 345
C355 (vs. gap) modified: Disulfide link with 361
C361 (≠ T304) modified: Disulfide link with 355
N363 (≠ D306) mutation to A: Loss of water permeation.
L369 (= L312) to S: in GGM; loss of activity
R379 (= R322) to Q: in GGM; loss of activity
A388 (= A331) to V: in GGM; loss of activity
S396 (= S339) mutation to A: Loss of activity.
F405 (≠ V348) to S: in GGM; loss of activity
A411 (≠ E354) to T: in GGM; slightly decreased activity; dbSNP:rs17683430
G426 (= G368) to R: in GGM; loss of activity
Q451 (vs. gap) mutation to A: Strong reduction in water permeation.
L452 (= L392) mutation to A: Loss of water permeation.
D454 (≠ S394) mutation to A: Has no effect on water permeation.
Q457 (= Q397) mutation to A: Loss of D-glucose transporter activity.; mutation to C: Strong reduction in D-glucose transporter activity.
T460 (≠ L400) mutation to A: Loss of D-glucose transporter activity.
V470 (= V410) to N: in GGM; requires 2 nucleotide substitutions; about 90% reduction in activity
R499 (≠ L439) to H: in GGM; impairs trafficking to the plasma membrane; decreases the sugar affinity
C511 (≠ G451) modified: Disulfide link with 255
C517 (≠ L454) modified: Disulfide link with 522
C522 (vs. gap) modified: Disulfide link with 517

Sites not aligning to the query:

191:664 natural variant: Missing (in GGM; loss of activity)
379:664 natural variant: Missing (in GGM; loss of activity)
615 H → Q: in GGM; slightly decreased activity
641 W→A: Slightly reduced D-glucose transporter activity.
660:661 HA→WG: Loss of D-glucose transporter activity.

P11170 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
32% identity, 92% coverage: 12:500/533 of query aligns to 28:567/662 of P11170

query
sites
P11170

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x
T

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S

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Y

F

I

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A

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N

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G

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F

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L

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E

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G

A

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C

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M

-

E

-

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I

-

I

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C255 (≠ L226) modified: Disulfide link with 608
Q457 (= Q397) mutation to W: Drasticly decreased affinity for glucose and phlorizin.
T460 (≠ L400) mutation to W: Decreased affinity for glucose and phlorizin.

Sites not aligning to the query:

608 modified: Disulfide link with 255

Q9NY91 Probable glucose sensor protein SLC5A4; Solute carrier family 5 member 4 from Homo sapiens (Human) (see paper)
31% identity, 91% coverage: 12:494/533 of query aligns to 28:561/659 of Q9NY91

query
sites
Q9NY91

D

D

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-

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Y

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A

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G

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D

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Y

W

L

P

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M

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G

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A

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S

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L

F

F

A

A

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G

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S

N

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F

Y

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A

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T

A

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A

A

A

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A

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Q

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A

A

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G

Y

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L

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I

-

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D

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W

E457 (≠ Q397) mutation to Q: Confers sugar transport activity not found in the wild-type protein. Increased sensitivity to inhibitor phlorizin.

7vsiA Structure of human sglt2-map17 complex bound with empagliflozin (see paper)
31% identity, 97% coverage: 12:526/533 of query aligns to 5:572/586 of 7vsiA

query
sites
7vsiA

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binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[[4-[(3S)-oxolan-3-yl]oxyphenyl]methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (= N61), H60 (≠ S66), G63 (= G69), L64 (= L70), T67 (≠ G73), V75 (≠ I81), F78 (≠ Y84), E79 (= E85), A82 (= A88), V137 (≠ G142), V266 (≠ L246), S267 (≠ G247), Y270 (= Y250), W271 (= W251), K301 (= K281), F433 (= F393), D434 (≠ S394), Q437 (= Q397), Y506 (= Y459)

P31639 Sodium/glucose cotransporter 2; Na(+)/glucose cotransporter 2; Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2 from Homo sapiens (Human) (see paper)
32% identity, 92% coverage: 12:500/533 of query aligns to 25:567/672 of P31639

query
sites
P31639

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H

F

F

V

V

G

G

L

L

M

A

G

G

G

T

A

G

Y

A

A

A

N

S

G

G

I

L

V

A

I
x
V

F

A

N

G

Y
x
F

E

E

W

W

T

N

A

A

A

L

L

F

V

V

L

V

I

L

L

L

F

L

A

G

I

W

F

L

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F

L

A

P

P

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Y

L

L

K

T

A

A

K

G

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T

V

M

P

P

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Q

F

Y

L

L

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R

Q

K

R

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Y

F

D

G

V

G

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R

S

R

R

I

R

R

A

L

F

Y

-

L

S

S

I

V

F

L

T

S

I

L

L

F

A

L

I

Y

L

I

F

F

I

T

D

K

T

I

A

S

G
x
V

A

D

L

M

Y

F

A

S

G

G

G

A

L

V

V

F

I

I

S

Q

N

Q

V

A

T

L

G

G

Y

W

L

-

N

N

L

I

W

Y

T

A

A

S

V

V

A

I

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A

L

L

A

L

L

G

V

I

A

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M

I

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Y

Y

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L

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G

L

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D

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L

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A

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I

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H

Y

D

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Q

L

L

L

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L

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D

T

D

G

D

D

F

-

L

L

P

P

W

W

T

P

G

A

-

L

L

L

W

L

G

G

V
x
L

V

T

L

I

L

V

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S

F

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Y

W

Y

Y

W

W

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N

D

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Q

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V

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Q

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K

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K

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L

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L

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M

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G

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M

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D

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M

M

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L

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M

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S

L

L

D

A

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A

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F

N

N

S

S

A

S

S

S

T

T

L

L

V

F

V

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K

M

D

D

F

I

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Y

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P

R

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L

W

R

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P

E

R

V

A

D

G

E

D

G

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K

E

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L

V

L

W

L

I

V

G

G

R

R

L

L

V

W

T

V

G

V

A

F

V

I

M

V

V

V

F

V

G

S

A

V

I

A

Y

W

A

L

P

P

S

V

I

V

A

Q

G

A

F

A

E

Q

-

G

S

Q

L

L

F
|
F

S

D

Y

Y

F

I

Q

Q

S

A

S

V

L

S

S

S

Y

Y

I

L

I

A

P

P

T

P

I

V

V

S

V

A

V

V

Y

F

I

V

V

L

G

A

L

L

F

F

V

V

P

P

W

R

L

V

N

N

G

E

N

Q

G

G

A

A

F

F

W

W

T

G

I

L

I

I

L

G

G

G

L

L

V

L

V

M

G

G

-

L

-

A

-

R

-

L

I

I

P

P

L

E

F

F

I

S

M

F

K

G

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V

G

T

S

G

C

V

V

W

Q

A

P

G

S

M

A

G

C

-

P

-

A

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F

L

L

P

C

E

G

I

V

H

H

Y

Y

T

L

I

Y

M

F

S

A

-

I

T

V

L

L

M

F

C

C

L

S

G

G

L

L

A

L

T

T

H

-

F

L

G

T

I

V

S

S

A

L

L

C

T

T

R

A

K

P

A

I

D

P

K

R

E

K

N

H

I

L

E

H

D

R

L

L

V

V

W

F

S

S

A

L

A

R

D

H

T

S

K

K

V95 (≠ I81) mutation to A: Strong reduction in D-glucose transporter activity. Confers partial resistance to empagliflozin inhibition.
F98 (≠ Y84) mutation to A: Slightly decreases D-glucose transporter activity. Abolishes the binding to inhibitor, empagliflozin.
V157 (≠ G142) mutation to A: Decreases D-glucose transporter activity.
L283 (≠ V243) mutation to M: Strong reduction in D-glucose transporter activity. Confers partial resistance to empagliflozin inhibition.
F453 (= F393) mutation to A: Slightly decreases D-glucose transporter activity. Greatly reduces the binding to inhibitor, empagliflozin.

Q9ET37 Solute carrier family 5 member 4A; SGLT3-a from Mus musculus (Mouse) (see paper)
32% identity, 92% coverage: 12:500/533 of query aligns to 28:567/656 of Q9ET37

query
sites
Q9ET37

D

D

Y

I

A

S

I

V

V

I

V

V

I

I

Y

Y

F

F

I

V

G

V

V

V

I

M

A

A

H

V

G

G

V

V

Y

W

V

A

S

M

R

L

K

K

-

T

N

N

E

R

E

S

G

T

A

V

D

G

G

G

Y

F

F

F

L

L

A

A

G

G

R

R

S

S

L

M

P

T

W

W

Y

W

L

P

I

M

G

G

F

A

S

S

L

L

F

F

A

A

S

S

N

N

M

I

S

G

G

S

S

G

S

H

F

F

V

V

G

G

L

L

M

A

G

G

G

T

A

G

Y

A

A

A

N

S

G

G

I

I

V

A

I

V

F

T

N

A

Y

F

E

E

W

S

T

H

A

S

-

F

A

A

L

L

V

L

L

L

I

V

L

L

F

G

A

W

I

I

F

F

V

V

L

-

P

P

S

I

F

Y

L

I

K

K

A

A

K

G

I

V

S

M

T

T

V

M

P

P

E

E

F

Y

L

L

E

K

Q

K

R

R

Y

F

D

G

V

G

R

K

S

R

R

L

R

Q

A

I

F

Y

-

L

S

S

I

I

F

L

T

F

I

L

L

F

A

I

I

C

L

V

F

I

I

L

D

T

T

I

A

S

G

A

A

D

L

I

Y

F

A

S

G

G

G

A

L

I

V

F

I

I

S

K

N

L

V

A

T

L

G

G

Y

L

L

N

N

L

L

Y

W

L

T

A

A

I

V

L

A

I

V

L

L

L

A

A

L

I

V

T

A

A

G

-

I

I

Y

F

T

T

I

I

L

T

G

G

L

L

S

A

A

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V

V

V

I

V

Y

T

T

D

D

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V

Q

Q

A

A

I

V

L

I

L

M

I

L

I

V

G

G

A

S

A

F

I

I

L

L

F

M

W

V

L

F

G

A

L

F

D

V

E

E

I

V

G

G

W

Y

E

E

-

S

-

F

-

T

Q

E

L

K

F

F

V

M

D

N

-

A

I

I

P

P

-

S

-

V

E

E

G

G

-

D

-

N

-

L

-

T

-

I

H

N

D

S

Q

R

L

C

I

Y

L

T

P

P

A

Q

D

P

D

D

D

S

F

F

-

H

-

I

-

F

-

R

-

D

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P

-

V

-

T

-

G

-

D

L

I

P

P

W

W

T

P

G

G

L

T

-

A

W

F

G

G

V

M

V

P

L

I

L

T

G

A

F

L

Y

W

Y

Y

W

W

T

C

I

I

N

N

Q

Q

F

V

V

I

V

V

Q

Q

R

R

T

C

L

L

G

C

A

G

K

K

N

N

L

L

K

S

E

H

G

V

Q

K

I

A

G

A

A

C

L

I

F

L

A

C

G

G

F

Y

L

L

K

K

L

L

P

L

N

P

I

L

F

F

L

F

M

M

I

V

I

M

P

P

G

G

V

M

I

I

A

S

L

R

K

I

L

L

Y

Y

P

T

E

D

L

M

-

V

-

A

-

C

-

V

-

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-

S

-

E

-

C

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-

K

-

H

-

C

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G

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D

-

V

E

G

T

C

P

T

D

N

L

Y

A

A

F

Y

P

P

T

M

L

L

A

V

F

L

E

K

L

L

M

M

P

P

I

M

G

G

V

L

R

R

G

G

L

L

I

M

M

L

A

S

A

V

L

M

I

L

A

A

A

S

I

L

M

M

S

S

L

L

D

T

S

S

A

V

M

F

N

N

S

S

A

A

S

S

T

T

L

L

V

F

V

T

K

I

D

D

F

L

V

Y

E

T

P

K

I

I

W

R

E

K

V

K

D

A

E

S

G

E

K

R

Q

E

V

L

W

L

I

I

-

A

G

G

R

R

L

L

V

F

T

V

G

S

A

V

V

L

M

I

V

V

F

T

G

S

A

I

I

L

Y

W

A

V

P

P

-

I

-

V

S

E

I

V

A

S

G

Q

F

G

E

G

S

Q

L

L

F

V

S

H

Y

Y

F

T

Q
x
E

S

A

S

I

L

S

S

S

Y

Y

I

L

I

G

P

P

T

P

I

I

V

A

V

V

Y

F

I

L

V

V

G

A

L

V

F

F

V

C

P

K

W

R

L

A

N

N

G

E

N

Q

G

G

A

A

F

F

W

W

T

G

I

L

I

M

L

V

G

G

L

L

V

V

V

M

G

G

I

L

P

I

L

R

F

M

I

I

M

A

K

E

E

F

V

S

T

Y

G

G

-

T

-

G

-

S

V

C

W

L

A

A

G

P

M

S

G

S

L

C

P

P

E

K

I

I

-

I

-

C

-

G

-

V

H

H

Y

Y

T

L

I

Y

M

F

S

A

T

I

L

I

M

L

M

F

C

F

L

V

G

C

L

I

A

L

T

V

H

I

F

L

G

G

I

V

S

S

A

Y

L

L

T

T

R

K

K

P

A

I

D

P

K

D

E

V

N

H

I

L

E

H

D

R

L

L

V

C

W

W

S

A

A

L

A

R

D

N

T

S

K

K

E457 (≠ Q397) mutation to Q: Confers sodium-dependent sugar transport activity not found in the wild type protein.

7sl8A Cryoem structure of sglt1 at 3.4 a resolution (see paper)
32% identity, 94% coverage: 12:512/533 of query aligns to 9:552/582 of 7sl8A

query
sites
7sl8A

D

D

Y

I

A

S

I

V

V

I

V

V

I

I

Y

Y

F

F

I

L

G

L

V

V

I

M

A

A

H

V

G

G

V

L

Y

W

V

S

S

M

R

F

K

K

N

-

E

-

G

-

A

-

D

-

G

-

Y

-

F

-

L

-

A

-

G

-

R

R

S

S

L

M

P

V

W
|
W

Y

W

L

P

I

I

G

G

F

A

S

S

L

L

F

F

A

A

S

S

N

N

M

I

S

G

G

S

S

G

S

H

F

F

V

I

G

G

L

L

M

A

G

G

G

T

A

G

Y

A

A

A

N

S

G

G

I

L

V

A

I

V

F

G

N

G

Y

F

E

E

W

W

T

N

A

A

A

L

L

V

V

L

L

L

I

L

L

V

F

L

A

G

I

W

F

V

V

F

L

V

P

P

S

I

F

Y

L

I

K

K

A

A

K

G

I

V

S

V

T

T

V

M

P

P

E

E

F

Y

L

L

E

R

Q

K

R

R

Y

F

-

G

D

G

V

Q

R

R

S

I

R

Q

R

V

A

Y

F

L

S

S

I

V

F

L

T

S

I

L

L

F

A

L

I

Y

L

I

F

F

I

T

D

K

T

I

A

S

G

V

A

D

L

I

Y

F