SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3610758 FitnessBrowser__Dino:3610758 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
30% identity, 77% coverage: 38:183/190 of query aligns to 32:182/203 of P07464

query
sites
P07464

L

L

V

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N

Y

Y

E

Y

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N

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Y

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S71 (= S68) binding
N85 (≠ G82) binding in other chain; binding in other chain
D93 (≠ T93) binding
H115 (≠ Y115) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A143) binding in other chain
A160 (= A161) binding in other chain
TK 165:166 (≠ RG 166:167) binding
R180 (= R181) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
183 binding in other chain

2xatA Complex of the hexapeptide xenobiotic acetyltransferase with chloramphenicol and desulfo-coenzyme a (see paper)
35% identity, 66% coverage: 65:189/190 of query aligns to 42:165/208 of 2xatA

query
sites
2xatA

A

A

A

R

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Y

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L

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binding desulfo-coenzyme a: G118 (= G142), T119 (≠ A143), G136 (= G160), S137 (≠ A161), G152 (≠ V176), G153 (= G177), I159 (≠ V183)

Sites not aligning to the query:

binding chloramphenicol: 6, 7, 8, 28

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
30% identity, 77% coverage: 38:183/190 of query aligns to 31:181/200 of 1krrA

query
sites
1krrA

L

L

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binding acetyl coenzyme *a: N84 (≠ G82), I103 (≠ F104), A104 (≠ G105), P105 (= P106), T112 (≠ A113), H114 (≠ Y115), G140 (= G142), S141 (≠ A143), N146 (≠ M148), G158 (= G160), A159 (= A161), A174 (≠ V176), P177 (= P179)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
30% identity, 77% coverage: 38:183/190 of query aligns to 31:181/201 of 1krvA

query
sites
1krvA

L

L

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binding 4-nitrophenyl beta-D-galactopyranoside: S70 (= S68), Y82 (≠ S80), N84 (≠ G82), V90 (≠ W88), D92 (≠ T93), M126 (= M128)
binding coenzyme a: A104 (≠ G105), S110 (≠ T111), T112 (≠ A113), W138 (= W140), G140 (= G142), S141 (≠ A143), N146 (≠ M148), A159 (= A161), P177 (= P179), R179 (= R181)

Sites not aligning to the query:

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
30% identity, 77% coverage: 38:183/190 of query aligns to 31:181/201 of 1kruA

query
sites
1kruA

L

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binding coenzyme a: H114 (≠ Y115), W138 (= W140), G140 (= G142), S141 (≠ A143), G158 (= G160), A159 (= A161), P177 (= P179)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: W62 (≠ R60), S70 (= S68), Y82 (≠ S80), Y82 (≠ S80), N84 (≠ G82), V90 (≠ W88), D92 (≠ T93), L102 (= L103), H114 (≠ Y115), M126 (= M128)

Sites not aligning to the query:

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
32% identity, 69% coverage: 52:183/190 of query aligns to 74:181/190 of 5u2kA

query
sites
5u2kA

S

N

L

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R

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L

L

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W
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Y

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A

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I

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I

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A

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D

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A

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L

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L

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K

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K

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V

binding coenzyme a: M90 (≠ S68), Y110 (≠ W88), A112 (≠ G90), H114 (≠ G92), L146 (≠ M148), T164 (≠ R166), K165 (≠ G167)

P50870 Streptogramin A acetyltransferase; Virginiamycin acetyltransferase D; Vat(D); EC 2.3.1.- from Enterococcus faecium (Streptococcus faecium) (see paper)
35% identity, 59% coverage: 75:186/190 of query aligns to 57:167/209 of P50870

query
sites
P50870

I

L

G

N

D

D

R

K

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S

K

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A

K

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K

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K

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Q

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R

H82 (≠ A102) mutation to A: 105-fold decrease in activity.

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
35% identity, 59% coverage: 75:186/190 of query aligns to 57:167/204 of 1mrlA

query
sites
1mrlA

I

L

G

N

D

D

R

K

V

L

S

K

I

I

G

G

A

K

H

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C

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P

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I

N

G

G

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x
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G

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K

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K

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G

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K

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D

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G

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A

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G

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V

V

R

V

G

K

E

D

I

I

P

A

A

P

G

Y

A

M

I

L

A

A

V

G

G

G

V

N

P

P

A

A

R

N

V

E

V

I

G

K

Q

Q

R

R

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: N81 (≠ D101), H82 (≠ A102), L93 (≠ A112), M102 (≠ S121), L108 (≠ A127)

Sites not aligning to the query:

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: 37, 39, 54, 56

3dhoA Structure of streptogramin acetyltransferase in complex with an inhibitor
35% identity, 59% coverage: 75:186/190 of query aligns to 57:167/203 of 3dhoA

query
sites
3dhoA

I

L

G

N

D

D

R

K

V

L

S

K

I

I

G

G

A

K

H

F

C

C

T

S

L

I

W

-

A

-

G

G

P

P

G

G

T

V

G

T

R

I

I

I

E

M

I

N

G

G

A

A

D

N

A

H

-

R

L
x
M

F

D

G

G

P

S

E

T

V

Y

F

P

V

F

T

N

A

L

A

F

G

G

Y

N

R

G

F

W

N

E

E

K

G

H

S

M

P

P

V

K

T

L

A

D

Q

Q

A

L

M

P

D

I

E

K

A

G

D

D

V

T

V

I

I

I

G

G

R

N

D

D

V

V

W

W

L

I

G

G

A

K

R

D

A

V

M

V

V

I

M

M

P

P

G

G

A

V

R

K

I

I

G

G

D

D

G

G

A

A

I
|
I

I

V

G
x
A

A

A

G

N

A

S

L

V

V

V

R
x
V

G

K

E

D

I

I

P

A

A

P

G

Y

A

M

I
x
L

A

A

V
x
G

G

G

V

N

P
|
P

A

A

R

N

V

E

V
x
I

G

K

Q

Q

R

R

binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: M84 (≠ L103), I139 (= I158), A141 (≠ G160), V147 (≠ R166), L155 (≠ I174), G157 (≠ V176), P160 (= P179), I164 (≠ V183)

1kk4A Crystal structure of vat(d) in complex with acetyl-coa (see paper)
35% identity, 59% coverage: 75:186/190 of query aligns to 57:167/205 of 1kk4A

query
sites
1kk4A

I

L

G

N

D

D

R

K

V

L

S

K

I

I

G

G

A

K

H

F

C

C

T

S

L
x
I

W

-

A

-

G
|
G

P

P

G

G

T

V

G

T

R

I

I

I

E

M

I

N

G

G

A

A

D

N

A

H

-

R

L

M

F

D

G

G

P

S

E

T

V

Y

F

P

V

F

T

N

A

L

A

F

G

G

Y

N

R

G

F

W

N

E

E

K

G

H

S

M

P

P

V

K

T

L

A

D

Q

Q

A

L

M

P

D

I

E
x
K

A

G

D

D

V

T

V

I

I

I

G

G

R

N

D

D

V

V

W
|
W

L

I

G
|
G

A
x
K

R

D

A

V

M

V

V

I

M

M

P

P

G

G

A

V

R

K

I

I

G

G

D

D

G

G

A

A

I

I

I

V

G
x
A

A
|
A

G

N

A

S

L

V

V

V

R
x
V

G
x
K

E

D

I

I

P

A

A

P

G

Y

A

M

I
x
L

A

A

V
x
G

G
|
G

V

N

P
|
P

A

A

R

N

V

E

V
x
I

G

K

Q

Q

R

R

binding acetyl coenzyme *a: I69 (≠ L87), G70 (= G90), K111 (≠ E130), W121 (= W140), G123 (= G142), K124 (≠ A143), A141 (≠ G160), A142 (= A161), V147 (≠ R166), K148 (≠ G167), L155 (≠ I174), G157 (≠ V176), G158 (= G177), P160 (= P179), I164 (≠ V183)

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
35% identity, 59% coverage: 75:186/190 of query aligns to 57:167/206 of 1khrA

query
sites
1khrA

I

L

G

N

D

D

R

K

V

L

S

K

I

I

G

G

A

K

H

F

C

C

T

S

L

I

W

-

A

-

G

G

P

P

G

G

T

V

G

T

R

I

I

I

E

M

I

N

G

G

A
|
A

D
x
N

A
x
H

-

R

L
x
M

F

D

G

G

P

S

E

T

V

Y

F

P

V

F

T

N

A
x
L

A

F

G

G

Y

N

R

G

F

W

N

E

E

K

G

H

S

M

P

P

V

K

T

L

A

D

Q

Q

A

L

M

P

D

I

E

K

A

G

D

D

V

T

V

I

I

I

G

G

R

N

D

D

V

V

W
|
W

L

I

G

G

A

K

R

D

A

V

M

V

V

I

M

M

P

P

G

G

A

V

R

K

I

I

G

G

D

D

G

G

A

A

I

I

I

V

G
x
A

A
|
A

G

N

A

S

L

V

V

V

R
x
V

G

K

E

D

I

I

P

A

A

P

G

Y

A

M

I
x
L

A

A

V
x
G

G

G

V

N

P
|
P

A

A

R

N

V

E

V
x
I

G

K

Q

Q

R

R

binding coenzyme a: H82 (≠ A102), M84 (≠ L103), W121 (= W140), A141 (≠ G160), A142 (= A161), V147 (≠ R166), L155 (≠ I174), G157 (≠ V176), P160 (= P179), I164 (≠ V183)
binding virginiamycin m1: A80 (= A100), N81 (≠ D101), H82 (≠ A102), L93 (≠ A112)

Sites not aligning to the query:

binding virginiamycin m1: 37, 39, 54

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
29% identity, 69% coverage: 55:185/190 of query aligns to 57:183/186 of 4isxA

query
sites
4isxA

L

V

G

G

R

K

D

Q

V

I

R

N

L

V

S

E

P

Q

N

N

A

I

A

R

F

C

S

D

N

Y

P

G

E

Y

R

N

I

I

E

H

I

V

G

G

D

E

R

N

V

F

S

F

I

A

G
x
N

A

Y

H

D

C

C

T

I

L

F

W

L

A

-

G

-

P

-

G

D

T

V

G

C

R

K

I

I

E

E

I

I

G

G

A

D

D

N

A

V

L

M

F

L

G
x
A

P

P

E

N

V

V

F

Q

V

I

T

Y

A

T

A

A

G

Y

Y

H

R

-

F

-

N

-

E

-

G

-

S

-

P

P

V

I

T

D

A

A

Q

Q

A

L

M

R

D

N

E

S

-

G

-

I

-

E

-

Y

-

G

A

S

D

P

V

V

V

K

I

I

G

G

R

D

D

N

V

V

W
|
W

L

I

G
|
G

A
x
G

R

G

A

V

M

I

V

I

M

T

P

P

G

G

A

I

R

T

I

I

G

G

D

D

G

N

A

V

I

V

I

I

G

G

A
|
A

G

G

A

S

L

V

V

V

R

T

G

K

E

D

I

I

P

P

A

P

G

N

A

T

I

V

A

A

V
|
V

G

G

V

N

P

P

A

C

R

R

V

V

V

I

G
x
K

Q

K

binding acetyl coenzyme *a: N84 (≠ G82), A104 (≠ G105), W138 (= W140), G140 (= G142), G141 (≠ A143), A159 (= A161), V174 (= V176), K182 (≠ G184)

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
29% identity, 71% coverage: 55:189/190 of query aligns to 31:146/290 of 4mzuB

query
sites
4mzuB

L

I

G

G

R

R

D

N

V

C

R

N

L

I

S
x
C

P
x
A

N

N

A

S

A

L

F

I

S

E

N

N

P

D

E

-

R

-

I

V

E

V

I

I

G

G

D

D

R

N

V

V

S

T

I

I

G

K

A

S

H
x
G

C

V

T

Q

L

I

W

W

A

D

G

G

P

-

G

-

T

-

G

-

R

-

I

I

E

H

I

I

G

Q

A

D

D

D

A

V

L
x
F

F

I

G

G

P

P

E
x
N

V

V

F

T

V

F

T
|
T

A

-

G

-

Y

-

R

-

F

-

N
|
N

E
x
D

G
x
K

S

Q

P
|
P

V

R

T

S

A

L

Q

K

A

T

M

-

D

-

E

-

A

-

D

-

V

-

V

I

I

V

G

K

R

K

D

G

V

A

W

S

L

I

G
|
G

A
|
A

R

N

A

S

M

T

V

I

M

L

P

P

G

G

A

I

R

L

I

I

G

G

D

E

G

N

A

A

I

M

I

V

G
|
G

A
|
A

G

G

A

A

L

V

V

I

R
x
T

G
x
K

E

N

I

V

P

P

A

D

G

N

A

A

I

I

A

V

V
x
I

G

G

V

N

P

P

A

G

R
|
R

V

I

V

T

G

G

Q

Y

R

V

A

E

A

A

D

N

binding coenzyme a: F72 (≠ L103), T80 (= T111), N81 (= N118), D82 (≠ E119), K83 (≠ G120), P85 (= P122), G99 (= G142), A100 (= A143), G117 (= G160), A118 (= A161), T123 (≠ R166), K124 (≠ G167), I133 (≠ V176), R138 (= R181)
binding magnesium ion: G58 (≠ H84), N76 (≠ E107)
binding thymine: C38 (≠ S62), A39 (≠ P63)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymine: 20, 21
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
29% identity, 68% coverage: 55:184/190 of query aligns to 31:142/294 of 4mzuF

query
sites
4mzuF

L

I

G

G

R

R

D

N

V

C

R

N

L

I

S
x
C

P

A

N

N

A

S

A

L

F

I

S

E

N

N

P

D

E

-

R

-

I

V

E

V

I

I

G

G

D

D

R

N

V

V

S

T

I

I

G

K

A

S

H

G

C

V

T

Q

L

I

W

W

A

D

G

G

P

-

G

-

T

-

G

-

R

-

I

I

E

H

I

I

G

Q

A

D

D

D

A

V

L

F

F

I

G

G

P

P

E

N

V

V

F

T

V

F

T

T

A

-

G

-

Y

-

R

-

F

-

N
|
N

E
x
D

G
x
K

S

Q

P

P

V

R

T

S

A

-

Q

-

A

-

M

-

D

-

E

K

A

I

D

K

V

T

V

I

I

V

G

K

R

K

D

G

V

A

W

S

L

I

G
|
G

A
|
A

R

N

A

S

M

T

V

I

M
x
L

P

P

G

G

A

I

R

L

I

I

G

G

D

E

G

N

A

A

I

M

I

V

G
|
G

A
|
A

G

G

A

A

L

V

V

I

R
x
T

G
x
K

E

N

I

V

P

P

A

D

G

N

A

A

I

I

A

V

V
x
I

G

G

V
x
N

P

P

A

G

R
|
R

V

I

V

T

G

G

binding coenzyme a: N81 (= N118), D82 (≠ E119), K83 (≠ G120), G100 (= G142), A101 (= A143), L106 (≠ M148), G118 (= G160), A119 (= A161), T124 (≠ R166), K125 (≠ G167), I134 (≠ V176), N136 (≠ V178), R139 (= R181)
binding thymine: C38 (≠ S62)

Sites not aligning to the query:

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
34% identity, 61% coverage: 75:189/190 of query aligns to 56:169/212 of 4husA

query
sites
4husA

I

I

G

G

D

D

R

K

V

L

S

I

I

I

G

G

A

R

H

F

C

C

T

S

L

I

W

-

A

-

G

G

P

P

G

G

T

T

G

T

R

F

I

I

E

M

I

N

G

G

A

A

D

N

A

H

L

R

F

M

G

D

P

G

E

S

V

T

F

Y

-

P

-

F

-

H

V

L

T

F

A

R

A

M

G

G

Y

W

R

E

F

-

N

-

E

K

G

Y

S

M

P

P

V

S

T

L

A

K

Q

D

A

L

M

P

D

L

E

K

A

G

D

D

V

I

V

E

I

I

G

G

R

N

D

D

V

V

W

W

L

I

G

G

A

R

R

D

A

V

M

T

V

I

M

M

P

P

G

G

A

V

R

K

I

I

G

G

D

D

G

G

A

A

I

I

G

A

A

A

G

E

A

A

L

V

V

V

R

T

G

K

E

N

I

V

P

A

A

P

G

Y

A

S

I

I

A

V

V

G

G

G

V

N

P

P

A

L

R

K

V

F

V

I

G

R

Q

K

R

R

A

F

A

S

D

D

Sites not aligning to the query:

binding virginiamycin m1: 17, 36, 38

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
34% identity, 61% coverage: 75:189/190 of query aligns to 56:169/211 of 4hurA

query
sites
4hurA

I

I

G

G

D

D

R

K

V

L

S

I

I

I

G

G

A

R

H

F

C

C

T
x
S

L
x
I

W

-

A

-

G
|
G

P

P

G

G

T

T

G

T

R

F

I

I

E

M

I

N

G

G

A
|
A

D
x
N

A

H

L

R

F

M

G

D

P

G

E

S

V

T

F

Y

-

P

-

F

-

H

V

L

T

F

A

R

A

M

G

G

Y

W

R

E

F

-

N

-

E

K

G

Y

S

M

P

P

V

S

T

L

A

K

Q

D

A

L

M

P

D

L

E
x
K

A

G

D

D

V

I

V

E

I

I

G

G

R

N

D

D

V

V

W
|
W

L
x
I

G
|
G

A
x
R

R

D

A

V

M

T

V

I

M
|
M

P

P

G

G

A

V

R

K

I

I

G

G

D

D

G

G

A

A

I

I

G
x
A

A
|
A

G

E

A

A

L

V

V

V

R
x
T

G

K

E

N

I

V

P

A

A

P

G

Y

A

S

I

I

A

V

V
x
G

G

G

V

N

P
|
P

A

L

R

K

V

F

V
x
I

G
x
R

Q

K

R

R

A

F

A

S

D

D

binding acetyl coenzyme *a: S67 (≠ T86), I68 (≠ L87), G69 (= G90), A79 (= A100), N80 (≠ D101), K110 (≠ E130), W120 (= W140), I121 (≠ L141), G122 (= G142), R123 (≠ A143), M128 (= M148), A140 (≠ G160), A141 (= A161), T146 (≠ R166), G156 (≠ V176), P159 (= P179), I163 (≠ V183), R164 (≠ G184)

Sites not aligning to the query:

binding acetyl coenzyme *a: 51

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
34% identity, 61% coverage: 75:189/190 of query aligns to 56:169/206 of 6x3jA

query
sites
6x3jA

I

I

G

G

D

D

R

K

V

L

S

I

I

I

G

G

A

R

H

F

C

C

T

S

L

I

W

-

A

-

G
|
G

P

P

G

G

T

T

G

T

R

F

I

I

E

M

I

N

G

G

A
|
A

D

N

A
x
H

L

R

F

M

G

D

P

G

E

S

V

T

F

Y

-

P

-

F

-
x
H

V
x
L

T

F

A

R

A

M

G

G

Y

W

R

E

F

-

N

-

E

K

G

Y

S
x
M

P
|
P

V

S

T

L

A

K

Q

D

A
x
L

M

P

D

L

E

K

A

G

D

D

V

I

V

E

I

I

G

G

R

N

D

D

V

V

W
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W

L

I

G
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G

A

R

R

D

A

V

M

T

V

I

M

M

P

P

G

G

A

V

R

K

I

I

G

G

D

D

G

G

A

A

I

I

G

A

A

A

G

E

A

A

L

V

V

V

R

T

G

K

E

N

I

V

P

A

A

P

G

Y

A

S

I

I

A

V

V

G

G

G

V

N

P

P

A

L

R

K

V

F

V

I

G

R

Q

K

R

R

A

F

A

S

D

D

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: H91 (vs. gap), L92 (≠ V110), M101 (≠ S121), P102 (= P122), L107 (≠ A127)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (= G90), A79 (= A100), H81 (≠ A102), W120 (= W140), G122 (= G142)

Sites not aligning to the query:

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: 51, 53, 55

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
34% identity, 61% coverage: 75:189/190 of query aligns to 56:169/207 of 6x3cA

query
sites
6x3cA

I

I

G

G

D

D

R

K

V

L

S

I

I

I

G

G

A

R

H

F

C

C

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L

I

W

-

A

-

G
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G

P

P

G

G

T

T

G

T

R

F

I

I

E

M

I

N

G
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G

A

A

D
x
N

A
x
H

L

R

F

M

G

D

P

G

E

S

V

T

F

Y

-

P

-

F

-
x
H

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x
L

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A

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G

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N

-

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x
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P

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x
L

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A

L

M

P

D

L

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K

A

G

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D

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I

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E

I

I

G

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N

D

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W
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W

L

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G

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x
R

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A

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M

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V

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M
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M

P

P

G

G

A

V

R

K

I

I

G

G

D

D

G

G

A

A

I

I

G
x
A

A
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A

G

E

A

A

L

V

V

V

R

T

G

K

E

N

I

V

P

A

A

P

G

Y

A

S

I

I

A

V

V

G

G

G

V

N

P

P

A

L

R

K

V

F

V

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G

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Q

K

R

R

A

F

A

S

D

D

binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: G78 (= G99), N80 (≠ D101), H81 (≠ A102), H91 (vs. gap), L92 (≠ V110), M101 (≠ S121), P102 (= P122), L104 (≠ T124)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (= G90), N80 (≠ D101), H81 (≠ A102), W120 (= W140), G122 (= G142), R123 (≠ A143), M128 (= M148), A140 (≠ G160), A141 (= A161)

Sites not aligning to the query:

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
34% identity, 61% coverage: 75:189/190 of query aligns to 56:169/203 of 6x3cE

query
sites
6x3cE

I

I

G

G

D

D

R

K

V

L

S

I

I

I

G

G

A

R

H

F

C

C

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-

A

-

G
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G

P

P

G

G

T

T

G

T

R

F

I

I

E

M

I

N

G
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G

A

A

D
x
N

A
x
H

L

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F
x
M

G

D

P

G

E

S

V

T

F

Y

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P

-

F

-
x
H

V
x
L

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F

A

R

A

M

G

G

Y

W

R

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F

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N

-

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x
M

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P

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L

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A

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L

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I

I

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N

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W
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W

L

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G

A

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R

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A

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M

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V

I

M

M

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P

G

G

A

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K

I

I

G

G

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D

G

G

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A

I

I

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x
A

A
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A

G

E

A

A

L

V

V

V

R

T

G

K

E

N

I

V

P

A

A

P

G

Y

A

S

I

I

A

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V

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G

G

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x
N

P
|
P

A

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K

V

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V

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R

R

A

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D

D

binding magnesium ion: N158 (≠ V178), P159 (= P179)
binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: G78 (= G99), N80 (≠ D101), H81 (≠ A102), H91 (vs. gap), L92 (≠ V110), M101 (≠ S121), P102 (= P122)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (= G90), H81 (≠ A102), M83 (≠ F104), W120 (= W140), G122 (= G142), A140 (≠ G160), A141 (= A161)

Sites not aligning to the query:

3vbjA Crystal structure of antd, an n-acyltransferase from bacillus cereus in complex with dtdp and 3-hydroxybutyryl-coa (see paper)
26% identity, 71% coverage: 53:186/190 of query aligns to 16:171/186 of 3vbjA

query
sites
3vbjA

L

L

R

S

L

V

G

G

R

K

D

N

V

V

R

L

L

I

S

S

P

K

N

K

A

A

A

S

F

I

S
x
Y

N

N

P

P

E

G

R

V

I

I

E

S

I

I

G

G

D

N

R

N

V

V

S
x
R

I

I

G
x
D

A
x
D

H

F

C

C

T

I

L

L

W

-

A

-

G

-

P

-

G

-

T
x
S

G

G

R

K

I

V

E

T

I

I

G

G

A

S

-

Y

-

S

-
x
H

-

I

-

A

-

Y

D

T

A

A

L

L

F

Y

G

G

P

G

E

E

V

V

F

G

V

I

T

E

A

M

A

Y

G

D

Y

F

R

A

F

N

N
x
I

E
x
S

G

S

S

R

P

T

V

I

T

V

A
x
Y

Q

A

A

A

M

I

D
|
D

E

D

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x
F

-

S

-

G

-

N

-

A

-

L

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M

-

G

-

P

-

T

-

I

-

P

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N

-

Q

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Y

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K

-

N

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V

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K

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T

A

G

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V

V

I

I

L

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D

H

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W

I

L

I

G

G

A

A

R

H

A

S

M

I

V

I

M
x
F

P

P

G

N

A

V

R

V

I

I

G

G

D

E

G

G

A

V

I

A

I

V

G
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G

A
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A

G

M

A

S

L

M

V

V

R
x
K

G

E

E

S

I

L

P

D

A

D

G

W

A

Y

I

I

A

Y

V
|
V

G

G

V
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V

P

P

A

V

R

R

V

K

V

I

G
x
K

Q

A

R

R

binding 3-hydroxybutanoyl-coenzyme a: H61 (vs. gap), I83 (≠ N118), S84 (≠ E119), Y90 (≠ A125), D94 (= D129), F96 (vs. gap), F133 (≠ M148), G145 (= G160), A146 (= A161), K151 (≠ R166), V161 (= V176), V163 (= V178), K169 (≠ G184)
binding thymidine-5'-diphosphate: Y31 (≠ S68), R43 (≠ S80), D45 (≠ G82), D46 (≠ A83), S51 (≠ T93)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 4

Query Sequence

>3610758 FitnessBrowser__Dino:3610758
MSETDPHPRKKSLSYPARVLRGIGAMLDPRAWAHAVRLVNYYNYSHVIPRRSLRLGRDVR
LSPNAAFSNPERIEIGDRVSIGAHCTLWAGPGTGRIEIGADALFGPEVFVTAAGYRFNEG
SPVTAQAMDEADVVIGRDVWLGARAMVMPGARIGDGAIIGAGALVRGEIPAGAIAVGVPA
RVVGQRAADP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory